Rh4CG381700

Squamosa promoter-binding-like protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005916
Physical Location & Seq
Forward (+)
63546932 .. 63550410
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG381700.1

Sequence Viewer

Length: 1728 bp
ATGAAGTTTTATTGCAAAGGCTCTGTTTGGTTGTTGAGGAAAACAAAAATGGAGTGGCAATCTGCAGCAGGCTATGCATTTTTCTTTGCTTGGTTTTCTTCTTCTTCTTTGTTCTTTACTGGGGGATCAAGAACCAGAGAAATTCAACCAGGCATTTCTGTATTAGTGTCACTGCATAATTGCTGTGACTTTTTGGTTCTGTGTTACATACTCATGGAGCCTTGGAGTTTCGGTTCAGAAGGGAAAGGGTTTTTACTTGCTGATGAGATGGATATACCAATTGATGCATTTGCAAGGAGTACAAAAGCATTCCTAGAATGGGATAACAAAACTTGCTTTAATTTCGATAAAGAATCAGTTGAGAGCATGGAGTTCATGGATTTGGGATTGCCTGATACAGTGAGGGAGCCTTGTCATGGTAACCAAGTAGTGGATGTCTTGAGTGCTGTCGCTGGTGTGGGCTGTAATGATTCTAGTAACAATACCGTAGCTTCTCCTACTTGTTTGATGACTTCAAGTTCATCTTGGGGTGAAGGAGAATCTGGCTCCAAATTTTCGAGTCCAGTCTGTGAGTCTAGTAGCCAGGATTCATCACTTATTGCTTTGAAGCTAGGGAGGTTGGCTGATAGCAAATATGGACAGGATAGTCAACATCTGAAAGAGAGGTCAAAGAGAGCTCGCACAAAGGGTCCACACTATCAGACTCTCACCTGCCAAGTTCATGGTTGTTACATGGATCTTAGCTCTTCAAAGGAGTACCATAAGAGGCATAGAGTTTGTGATGCTCACTCAAAGACTGCAATAGTTATCGTTAATGGCATTGAGCAGAGGTTTTGTCAACAATGTAGCAGGTTTCATCTGCTGGCTGAATTCGATGATGTTAAGCGCAGTTGTCGTAGACGCTTAGCTGGCCACAATCAACGCCGCAGGAAGCCTCAACTAGATACTTTTGATTGTAAATCGCGTAAGTGGCTTCAGTCATATCAAGGCATTGGATATCTTGGAACTTCCTTGCCAAAGAGAACACCGTTTGTCTTTTCAGATATACTTCCAGGTGGCATTCTATATCCAGAAGAATCTAACGAGTATGGACATATTAAAACTGAAGATGAATCAACTTACAGTCAATGTGTGACACCCATAACAAACAGGCTATTACCTCCAAACTCTTTCCTCCAACTGCATGGCCTCGGGAAGCAACATGCTTCAGAAGTTTCTTCATCAGGAAGTGAATTCTCGGTTTTTGATACTGCATCACATGTCCAGAAAGCATCTGGGGCGTCAACCTACCAGGGTGCTCTCTCTCTTCTGTCAGCTCAATCACAGAGCTCAATCCATTCAGCAAGAAATCAAGTGGCTACCCCTCTGCAAATACAGAGAATCCATCATGGTGCTGGTCAAATTAATGAACGGCCTTCCACAGTAAAAGCCTTGGAAAAATATGGACGAAACAGGTCCTTCCCATGCAGGATAAACTCCATGGGAGCTGACCAGATGGACTCAGTAATGATTTCTGATTCTAGTCATGCTGTTGACTTCCAACTTCACACAGATAGGGTTTTCCAGGAGTCAGCTCGCTTCAGTCACAAGTATCATCTTTCTCCTGAAAATGGACCAACTTTTGATTTGCTCCAATTGTCTTCACATTTTCAAAGAGTGGAGCAAGAGAGAAACTTTTTGCAAGTCAAACATGAGCAGGGGGATTTCTACTGTTTCCCGCAATCTTAA

Protein Analysis

575

Amino Acids

64.55

Weight (kDa)

7.82

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 238 - 311 2.9e-31 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 719
AasI GACNNNNNNGTC 1 cut(s) 645
Acc36I ACCTGC 2 cut(s) 719, 840
AccB7I CCANNNNNTGG 1 cut(s) 430
AccI GTMKAC 1 cut(s) 898
AccII CGCG 1 cut(s) 964
AciI CCGC 2 cut(s) 925, 1718
AclWI GGATC 2 cut(s) 133, 744
AcoI YGGCCR 1 cut(s) 910
AcsI RAATTY 4 cut(s) 141, 551, 869, 1232
AcuI CTGAAG 4 cut(s) 959, 1125, 1191, 1564
AcyI GRCGYC 1 cut(s) 1280
AfaI GTAC 2 cut(s) 301, 758
AfiI CCNNNNNNNGG 4 cut(s) 319, 416, 430, 1610
AflIII ACRYGT 1 cut(s) 1258
AgsI TTSAA 5 cut(s) 146, 516, 607, 750, 1652
AjnI CCWGG 5 cut(s) 148, 582, 1051, 1290, 1563
AloI GAACNNNNNNTCC 2 cut(s) 217, 249
AluBI AGCT 9 cut(s) 491, 610, 677, 744, 908, 1316, 1329, 1487, 1574
AluI AGCT 9 cut(s) 491, 610, 677, 744, 908, 1316, 1329, 1487, 1574
Alw21I GWGCWC 3 cut(s) 679, 1300, 1331
AlwI GGATC 2 cut(s) 133, 744
Ama87I CYCGRG 1 cut(s) 1190
AoxI GGCC 3 cut(s) 910, 1186, 1412
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 4 cut(s) 141, 551, 869, 1232
AseI ATTAAT 1 cut(s) 1404
AspLEI GCGC 1 cut(s) 888
AspS9I GGNCC 3 cut(s) 689, 1455, 1613
AsuHPI GGTGA 2 cut(s) 542, 700
AvaI CYCGRG 1 cut(s) 1190
AvaII GGWCC 3 cut(s) 689, 1455, 1613
BalI TGGCCA 1 cut(s) 912
BanII GRGCYC 2 cut(s) 679, 1331
BbsI GAAGAC 1 cut(s) 1632
Bbv12I GWGCWC 3 cut(s) 679, 1300, 1331
BbvI GCAGC 1 cut(s) 77
BccI CCATC 3 cut(s) 262, 1392, 1489
BceAI ACGGC 1 cut(s) 1427
BciT130I CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
BfaI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
BfmI CTRYAG 1 cut(s) 63
BfuAI ACCTGC 2 cut(s) 719, 840
BisI GCNGC 2 cut(s) 66, 925
BlpI GCTNAGC 1 cut(s) 904
BlsI GCNGC 2 cut(s) 67, 926
Bme1390I CCNGG 5 cut(s) 150, 584, 1053, 1292, 1565
Bme18I GGWCC 3 cut(s) 689, 1455, 1613
BmeT110I CYCGRG 1 cut(s) 1190
BmgT120I GGNCC 3 cut(s) 689, 1455, 1613
BmiI GGNNCC 4 cut(s) 219, 408, 547, 690
BmrFI CCNGG 5 cut(s) 150, 584, 1053, 1292, 1565
BmrI ACTGGG 1 cut(s) 129
BmsI GCATC 4 cut(s) 274, 772, 1262, 1280
BmuI ACTGGG 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 1632
Bpu1102I GCTNAGC 1 cut(s) 904
BpuEI CTTGAG 1 cut(s) 460
BsaHI GRCGYC 1 cut(s) 1280
BsaJI CCNNGG 5 cut(s) 221, 1189, 1291, 1431, 1479
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 416, 430, 1610
Bse1I ACTGG 2 cut(s) 124, 563
BseBI CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
BseDI CCNNGG 5 cut(s) 221, 1189, 1291, 1431, 1479
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 4 cut(s) 319, 416, 430, 1610
BseMII CTCAG 1 cut(s) 1515
BseNI ACTGG 2 cut(s) 124, 563
BseXI GCAGC 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 964
BshFI GGCC 3 cut(s) 912, 1188, 1414
BsiHKAI GWGCWC 3 cut(s) 679, 1300, 1331
BsiHKCI CYCGRG 1 cut(s) 1190
BslI CCNNNNNNNGG 4 cut(s) 319, 416, 430, 1610
BsmI GAATGC 2 cut(s) 308, 1059
BsnI GGCC 3 cut(s) 912, 1188, 1414
BsoBI CYCGRG 1 cut(s) 1190
Bsp1286I GDGCHC 3 cut(s) 679, 1300, 1331
Bsp143I GATC 2 cut(s) 125, 736
Bsp1720I GCTNAGC 1 cut(s) 904
Bsp19I CCATGG 1 cut(s) 1479
BspACI CCGC 2 cut(s) 925, 1718
BspANI GGCC 3 cut(s) 912, 1188, 1414
BspCNI CTCAG 1 cut(s) 1514
BspFNI CGCG 1 cut(s) 964
BspLI GGNNCC 4 cut(s) 219, 408, 547, 690
BspMAI CTGCAG 1 cut(s) 67
BspMI ACCTGC 2 cut(s) 719, 840
BspPI GGATC 2 cut(s) 133, 744
BspQI GCTCTTC 1 cut(s) 751
BsrI ACTGG 2 cut(s) 124, 563
BssECI CCNNGG 5 cut(s) 221, 1189, 1291, 1431, 1479
BssMI GATC 2 cut(s) 125, 736
BssNI GRCGYC 1 cut(s) 1280
BssT1I CCWWGG 3 cut(s) 221, 1431, 1479
Bst2UI CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
Bst4CI ACNGT 6 cut(s) 400, 487, 1029, 1124, 1423, 1712
Bst6I CTCTTC 2 cut(s) 751, 1311
BstACI GRCGYC 1 cut(s) 1280
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 5 cut(s) 70, 679, 864, 910, 1576
BstDEI CTNAG 3 cut(s) 740, 904, 1501
BstDSI CCRYGG 1 cut(s) 1479
BstEII GGTNACC 1 cut(s) 419
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 1 cut(s) 964
BstHHI GCGC 1 cut(s) 888
BstKTI GATC 2 cut(s) 128, 739
BstMBI GATC 2 cut(s) 125, 736
BstMWI GCNNNNNNNGC 4 cut(s) 74, 909, 970, 1277
BstNI CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
BstNSI RCATGY 2 cut(s) 1205, 1262
BstPI GGTNACC 1 cut(s) 419
BstSCI CCNGG 5 cut(s) 148, 582, 1051, 1290, 1563
BstSFI CTRYAG 1 cut(s) 63
BstUI CGCG 1 cut(s) 964
BstV1I GCAGC 1 cut(s) 77
BstV2I GAAGAC 1 cut(s) 1632
BstX2I RGATCY 1 cut(s) 736
BstXI CCANNNNNNTGG 2 cut(s) 722, 1184
BstYI RGATCY 1 cut(s) 736
BsuRI GGCC 3 cut(s) 912, 1188, 1414
BtgI CCRYGG 1 cut(s) 1479
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 170, 405
BveI ACCTGC 2 cut(s) 719, 840
Cac8I GCNNGC 5 cut(s) 70, 679, 864, 910, 1576
CfoI GCGC 1 cut(s) 888
Cfr13I GGNCC 3 cut(s) 689, 1455, 1613
CseI GACGC 2 cut(s) 909, 1269
Csp6I GTAC 2 cut(s) 300, 757
CviQI GTAC 2 cut(s) 300, 757
DdeI CTNAG 3 cut(s) 740, 904, 1501
DpnI GATC 2 cut(s) 127, 738
DpnII GATC 2 cut(s) 125, 736
DrdI GACNNNNNNGTC 1 cut(s) 645
DseDI GACNNNNNNGTC 1 cut(s) 645
EaeI YGGCCR 1 cut(s) 910
Eam1104I CTCTTC 2 cut(s) 751, 1311
EarI CTCTTC 2 cut(s) 751, 1311
Ecl136II GAGCTC 2 cut(s) 677, 1329
Eco130I CCWWGG 3 cut(s) 221, 1431, 1479
Eco24I GRGCYC 2 cut(s) 679, 1331
Eco32I GATATC 1 cut(s) 998
Eco47I GGWCC 3 cut(s) 689, 1455, 1613
Eco53kI GAGCTC 2 cut(s) 677, 1329
Eco57I CTGAAG 4 cut(s) 959, 1125, 1191, 1564
Eco88I CYCGRG 1 cut(s) 1190
Eco91I GGTNACC 1 cut(s) 419
EcoICRI GAGCTC 2 cut(s) 677, 1329
EcoO109I RGGNCCY 1 cut(s) 1455
EcoO65I GGTNACC 1 cut(s) 419
EcoRI GAATTC 2 cut(s) 869, 1232
EcoRII CCWGG 5 cut(s) 148, 582, 1051, 1290, 1563
EcoRV GATATC 1 cut(s) 998
EcoT14I CCWWGG 3 cut(s) 221, 1431, 1479
EcoT22I ATGCAT 2 cut(s) 79, 289
EcoT38I GRGCYC 2 cut(s) 679, 1331
ErhI CCWWGG 3 cut(s) 221, 1431, 1479
FalI AAGNNNNNCTT 2 cut(s) 508, 540
FauI CCCGC 1 cut(s) 1725
FblI GTMKAC 1 cut(s) 898
Fnu4HI GCNGC 2 cut(s) 66, 925
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 2 cut(s) 679, 1331
Fsp4HI GCNGC 2 cut(s) 66, 925
FspBI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
GlaI GCGC 1 cut(s) 887
GluI GCNGC 2 cut(s) 66, 925
HaeIII GGCC 3 cut(s) 912, 1188, 1414
HgaI GACGC 2 cut(s) 909, 1269
HhaI GCGC 1 cut(s) 888
Hin1I GRCGYC 1 cut(s) 1280
Hin6I GCGC 1 cut(s) 886
HinP1I GCGC 1 cut(s) 886
HincII GTYRAC 4 cut(s) 650, 839, 1284, 1534
HindII GTYRAC 4 cut(s) 650, 839, 1284, 1534
HphI GGTGA 2 cut(s) 542, 700
Hpy166II GTNNAC 6 cut(s) 650, 692, 839, 899, 1284, 1534
Hpy188I TCNGA 6 cut(s) 238, 657, 702, 1042, 1210, 1516
Hpy188III TCNNGA 7 cut(s) 129, 439, 1070, 1192, 1224, 1264, 1604
Hpy8I GTNNAC 6 cut(s) 650, 692, 839, 899, 1284, 1534
HpyAV CCTTC 4 cut(s) 233, 527, 1425, 1468
HpyCH4III ACNGT 6 cut(s) 400, 487, 1029, 1124, 1423, 1712
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 909, 970, 1277
HpyF3I CTNAG 3 cut(s) 740, 904, 1501
Hsp92I GRCGYC 1 cut(s) 1280
HspAI GCGC 1 cut(s) 886
Kzo9I GATC 2 cut(s) 125, 736
LguI GCTCTTC 1 cut(s) 751
LmnI GCTCC 6 cut(s) 217, 406, 551, 1484, 1635, 1660
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 4 cut(s) 274, 772, 1262, 1280
MaeI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
MaeIII GTNAC 8 cut(s) 168, 185, 203, 419, 476, 728, 1132, 1583
MalI GATC 2 cut(s) 127, 738
MboI GATC 2 cut(s) 125, 736
MboII GAAGA 9 cut(s) 90, 93, 96, 738, 1085, 1118, 1209, 1298, 1632
MfeI CAATTG 2 cut(s) 279, 1634
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 3 cut(s) 679, 1300, 1331
MlsI TGGCCA 1 cut(s) 912
MluCI AATT 9 cut(s) 141, 178, 279, 340, 551, 869, 1232, 1401, 1634
MluNI TGGCCA 1 cut(s) 912
MlyI GAGTC 5 cut(s) 568, 581, 697, 1493, 1577
MmeI TCCRAC 2 cut(s) 1201, 1564
Mox20I TGGCCA 1 cut(s) 912
Mph1103I ATGCAT 2 cut(s) 79, 289
MscI TGGCCA 1 cut(s) 912
MseI TTAA 6 cut(s) 339, 813, 882, 1098, 1404, 1726
MslI CAYNNNNRTG 2 cut(s) 212, 1389
Msp20I TGGCCA 1 cut(s) 912
MspR9I CCNGG 5 cut(s) 150, 584, 1053, 1292, 1565
MunI CAATTG 2 cut(s) 279, 1634
Mva1269I GAATGC 2 cut(s) 308, 1059
MvaI CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
MvnI CGCG 1 cut(s) 964
MwoI GCNNNNNNNGC 4 cut(s) 74, 909, 970, 1277
NcoI CCATGG 1 cut(s) 1479
NdeII GATC 2 cut(s) 125, 736
NlaIV GGNNCC 4 cut(s) 219, 408, 547, 690
NmuCI GTSAC 4 cut(s) 168, 185, 1132, 1583
NsiI ATGCAT 2 cut(s) 79, 289
NspI RCATGY 2 cut(s) 1205, 1262
PaqCI CACCTGC 1 cut(s) 719
PciI ACATGT 1 cut(s) 1258
PciSI GCTCTTC 1 cut(s) 751
PctI GAATGC 2 cut(s) 308, 1059
PfeI GAWTC 8 cut(s) 353, 470, 539, 587, 1076, 1112, 1380, 1517
PflMI CCANNNNNTGG 1 cut(s) 430
PfoI TCCNGGA 1 cut(s) 1563
PkrI GCNGC 2 cut(s) 67, 926
PleI GAGTC 5 cut(s) 567, 580, 697, 1493, 1576
PpsI GAGTC 5 cut(s) 567, 580, 697, 1493, 1576
PpuMI RGGWCCY 1 cut(s) 1455
PscI ACATGT 1 cut(s) 1258
PshBI ATTAAT 1 cut(s) 1404
Psp124BI GAGCTC 2 cut(s) 679, 1331
Psp5II RGGWCCY 1 cut(s) 1455
Psp6I CCWGG 5 cut(s) 148, 582, 1051, 1290, 1563
PspEI GGTNACC 1 cut(s) 419
PspGI CCWGG 5 cut(s) 148, 582, 1051, 1290, 1563
PspN4I GGNNCC 4 cut(s) 219, 408, 547, 690
PspPI GGNCC 3 cut(s) 689, 1455, 1613
PspPPI RGGWCCY 1 cut(s) 1455
PstI CTGCAG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 736
RsaI GTAC 2 cut(s) 301, 758
RsaNI GTAC 2 cut(s) 300, 757
RseI CAYNNNNRTG 2 cut(s) 212, 1389
SacI GAGCTC 2 cut(s) 679, 1331
SapI GCTCTTC 1 cut(s) 751
SaqAI TTAA 6 cut(s) 339, 813, 882, 1098, 1404, 1726
SatI GCNGC 2 cut(s) 66, 925
Sau3AI GATC 2 cut(s) 125, 736
Sau96I GGNCC 3 cut(s) 689, 1455, 1613
SchI GAGTC 5 cut(s) 568, 581, 697, 1493, 1577
ScrFI CCNGG 5 cut(s) 150, 584, 1053, 1292, 1565
SduI GDGCHC 3 cut(s) 679, 1300, 1331
SfaNI GCATC 4 cut(s) 274, 772, 1262, 1280
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 3 cut(s) 689, 1455, 1613
SmiMI CAYNNNNRTG 2 cut(s) 212, 1389
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 9 cut(s) 141, 178, 279, 340, 551, 869, 1232, 1401, 1634
SsiI CCGC 2 cut(s) 925, 1718
SspMI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
SstI GAGCTC 2 cut(s) 679, 1331
StyD4I CCNGG 5 cut(s) 148, 582, 1051, 1290, 1563
StyI CCWWGG 3 cut(s) 221, 1431, 1479
TaaI ACNGT 6 cut(s) 400, 487, 1029, 1124, 1423, 1712
TaqI TCGA 3 cut(s) 345, 557, 873
TasI AATT 9 cut(s) 141, 178, 279, 340, 551, 869, 1232, 1401, 1634
TatI WGTACW 1 cut(s) 299
TauI GCSGC 1 cut(s) 927
TfiI GAWTC 8 cut(s) 353, 470, 539, 587, 1076, 1112, 1380, 1517
Tru1I TTAA 6 cut(s) 339, 813, 882, 1098, 1404, 1726
Tru9I TTAA 6 cut(s) 339, 813, 882, 1098, 1404, 1726
TscAI CASTG 2 cut(s) 177, 405
TseFI GTSAC 4 cut(s) 168, 185, 1132, 1583
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 4 cut(s) 168, 185, 1132, 1583
TspDTI ATGAA 9 cut(s) 17, 364, 510, 579, 710, 845, 1125, 1209, 1422
TspRI CASTG 2 cut(s) 177, 405
Van91I CCANNNNNTGG 1 cut(s) 430
VpaK11BI GGWCC 3 cut(s) 689, 1455, 1613
VspI ATTAAT 1 cut(s) 1404
XapI RAATTY 4 cut(s) 141, 551, 869, 1232
XceI RCATGY 2 cut(s) 1205, 1262
XcmI CCANNNNNNNNNTGG 2 cut(s) 1271, 1391
XmiI GTMKAC 1 cut(s) 898
XspI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
Zsp2I ATGCAT 2 cut(s) 79, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.