Rh4AG356900

Squamosa promoter-binding-like protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005916
Physical Location & Seq
Forward (+)
66728849 .. 66733360
4512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG356900.1

Sequence Viewer

Length: 1740 bp
ATGTTTATGGGTGATGAAGTTTTATTGCAAAGGCTCTGTTTGGTTGTTGAGGAAAACAAAATGGAGTGGGAATCTGCAGCAGGCTATGCATTTTTCTTTGCTTGGTTTTCTTCTTCTTCTTTGTTCTTTACTGGGGGATCAAGAACCAGAGAAATTCAACCAGGCATTTCTGTATTAGTGTCACTGCATAATTCCTGTGACTTTTTGGTTCTGTGTTACATACTCATGGAGCCTTGGAGTTTCGGTTCAGAAGGGAAAGGGTTTTTACTTGCTGATGAGATGGATATACCAATTGATGCATTTGCAAGGAGTACAAAAGCATTCCTAGAATGGGATAACAAAACTTGCTTTAATTTCGATAAAGAATCAGTTGAGAACATGGAGTTCATGGATTTGGGATTGCCTGATACAGTGAGGGAGCCTTGTCATGGTAACCAAGTAGTGGATGTCTTGAGTGGTGTCGCTGGTGTGGGCTGTAATGATTCTAGTAACAATACCGTAGCTTCTCCTACTTGTTTGATGACTTCAAGTTCATCTTGGGGTGAAGGAGAATCTGGCTCCAAATTTTCGAGTCCAGTCTATGAGTCTAGTAGCCAGGATTCATCACTTATTGCTTTGAAGCTAGGGAGGTTGGCTGATAGCAAATATGGACAGGATAGTCAACATCTGAAAGAGAGGTCAAAGAGAGCTCGCACAAAGGGTCCACACTATCAGACTCTCACCTGCCAAGTTCATGGTTGTAACATGGATCTTAGCTCTTCAAAGGAGTACCATAAGAGGCATAGAGTTTGTGATGCTCACTCAAAGACTGCAATAGTTATCGTTAATGGCATTGAACAGAGGTTTTGTCAACAATGTAGCAGGTTTCATCTGCTGGCTGAATTCGATGATGTTAAGCGCAGTTGTCGTAGACGCTTAGCTGGCCACAATCAACGCCGCAGGAAGCCTCAACTAGATACTTTTGATTGTAAATCGCGTAAGTGGCTTCAGTCATATCAAGGCATTGGATATCTTGGAACTTCCTTGCCAAAGAGAACACCGTTTGTCTTTTCAGATATACTTCCAGGTGGCATTCTATATCCAGAAGAATCTAACGAGTATGGACATATTAAAACTGAAGATGAATCAACTTACAGTCAATGTGTGACACCCATAACAAACAGGCCATTACCTCCAAACTCTTTCCTCCAACTGCATGGCCTCGGGAAGCAACATGCTTCAGAAGTTCCTTCGTCAGGAAGTGAATTCTCAGTTTTTGATAGTGCATCACATGTCCAGAAAGCATCTGGGGCGTCAACCTACCAGGGTGCTCTCTCTCTTCTGTCAGCTCAATCACAGAGCTCAATCCATTCAGCAAGAAATCAAGTGGCTACCCCTCTGCAAATACAGAGAACCCATCATGGTGCTGGTCAAATTAATGAACGGCCTTCCACAGTAAAACCCTTGGAAAAATATGGACGAAACAGGTCCTTCCCATGCAGGATAAACTCCATGGGAACTGACCAGATGGACTCAGTAATGATTTCTGATTCTAGTCATGCTGTTGACTTCCAACTTCACACAGATAGGGTTTTCCAGGAGTCAGCTCGCTTCAGTCACAAGTATCATCTTTCTCCTGAAAATGGACCAACTTTTGATTTGCTCCAATTGTCTTCACATTTTCAAAGAGTGGAGCAAGAGAGAAACTTTTTGCAAATCAAACATGAGCAGGGGGATTTCTACTGTTTCCCGCAATCTTAA

Protein Analysis

579

Amino Acids

64.98

Weight (kDa)

6.45

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 242 - 315 1.1e-31 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 731
AasI GACNNNNNNGTC 1 cut(s) 657
Acc36I ACCTGC 2 cut(s) 731, 852
AccB7I CCANNNNNTGG 1 cut(s) 442
AccI GTMKAC 1 cut(s) 910
AccII CGCG 1 cut(s) 976
AciI CCGC 2 cut(s) 937, 1730
AclWI GGATC 2 cut(s) 145, 756
AcoI YGGCCR 1 cut(s) 922
AcsI RAATTY 4 cut(s) 153, 563, 881, 1244
AcuI CTGAAG 4 cut(s) 971, 1137, 1203, 1576
AcyI GRCGYC 1 cut(s) 1292
AfaI GTAC 2 cut(s) 313, 770
AfiI CCNNNNNNNGG 5 cut(s) 331, 428, 442, 1235, 1622
AflIII ACRYGT 1 cut(s) 1270
AgsI TTSAA 6 cut(s) 158, 528, 619, 762, 836, 1664
AjnI CCWGG 5 cut(s) 160, 594, 1063, 1302, 1575
AloI GAACNNNNNNTCC 2 cut(s) 229, 261
AluBI AGCT 8 cut(s) 503, 622, 689, 756, 920, 1328, 1341, 1586
AluI AGCT 8 cut(s) 503, 622, 689, 756, 920, 1328, 1341, 1586
Alw21I GWGCWC 3 cut(s) 691, 1312, 1343
AlwI GGATC 2 cut(s) 145, 756
Ama87I CYCGRG 1 cut(s) 1202
AoxI GGCC 4 cut(s) 922, 1163, 1198, 1424
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 4 cut(s) 153, 563, 881, 1244
AseI ATTAAT 1 cut(s) 1416
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 3 cut(s) 701, 1467, 1625
AsuHPI GGTGA 3 cut(s) 23, 554, 712
AvaI CYCGRG 1 cut(s) 1202
AvaII GGWCC 3 cut(s) 701, 1467, 1625
BalI TGGCCA 1 cut(s) 924
BanII GRGCYC 2 cut(s) 691, 1343
BbsI GAAGAC 1 cut(s) 1644
Bbv12I GWGCWC 3 cut(s) 691, 1312, 1343
BbvI GCAGC 1 cut(s) 89
BccI CCATC 3 cut(s) 274, 1404, 1501
BceAI ACGGC 1 cut(s) 1439
BciT130I CCWGG 5 cut(s) 162, 596, 1065, 1304, 1577
BfaI CTAG 6 cut(s) 326, 486, 588, 623, 953, 1533
BfmI CTRYAG 1 cut(s) 75
BfuAI ACCTGC 2 cut(s) 731, 852
BisI GCNGC 2 cut(s) 78, 937
BlpI GCTNAGC 1 cut(s) 916
BlsI GCNGC 2 cut(s) 79, 938
Bme1390I CCNGG 5 cut(s) 162, 596, 1065, 1304, 1577
Bme18I GGWCC 3 cut(s) 701, 1467, 1625
BmeT110I CYCGRG 1 cut(s) 1202
BmgT120I GGNCC 3 cut(s) 701, 1467, 1625
BmiI GGNNCC 4 cut(s) 231, 420, 559, 702
BmrFI CCNGG 5 cut(s) 162, 596, 1065, 1304, 1577
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 4 cut(s) 286, 784, 1274, 1292
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 1644
Bpu1102I GCTNAGC 1 cut(s) 916
BpuEI CTTGAG 1 cut(s) 472
BsaHI GRCGYC 1 cut(s) 1292
BsaJI CCNNGG 5 cut(s) 233, 1201, 1303, 1443, 1491
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 5 cut(s) 331, 428, 442, 1235, 1622
Bse1I ACTGG 2 cut(s) 136, 575
BseBI CCWGG 5 cut(s) 162, 596, 1065, 1304, 1577
BseDI CCNNGG 5 cut(s) 233, 1201, 1303, 1443, 1491
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 5 cut(s) 331, 428, 442, 1235, 1622
BseMII CTCAG 2 cut(s) 1263, 1527
BseNI ACTGG 2 cut(s) 136, 575
BseXI GCAGC 1 cut(s) 89
Bsh1236I CGCG 1 cut(s) 976
BshFI GGCC 4 cut(s) 924, 1165, 1200, 1426
BsiHKAI GWGCWC 3 cut(s) 691, 1312, 1343
BsiHKCI CYCGRG 1 cut(s) 1202
BslI CCNNNNNNNGG 5 cut(s) 331, 428, 442, 1235, 1622
BsmI GAATGC 2 cut(s) 320, 1071
BsnI GGCC 4 cut(s) 924, 1165, 1200, 1426
BsoBI CYCGRG 1 cut(s) 1202
Bsp1286I GDGCHC 3 cut(s) 691, 1312, 1343
Bsp143I GATC 2 cut(s) 137, 748
Bsp1720I GCTNAGC 1 cut(s) 916
Bsp19I CCATGG 1 cut(s) 1491
BspACI CCGC 2 cut(s) 937, 1730
BspANI GGCC 4 cut(s) 924, 1165, 1200, 1426
BspCNI CTCAG 2 cut(s) 1262, 1526
BspFNI CGCG 1 cut(s) 976
BspLI GGNNCC 4 cut(s) 231, 420, 559, 702
BspMAI CTGCAG 1 cut(s) 79
BspMI ACCTGC 2 cut(s) 731, 852
BspPI GGATC 2 cut(s) 145, 756
BspQI GCTCTTC 1 cut(s) 763
BsrI ACTGG 2 cut(s) 136, 575
BssECI CCNNGG 5 cut(s) 233, 1201, 1303, 1443, 1491
BssMI GATC 2 cut(s) 137, 748
BssNI GRCGYC 1 cut(s) 1292
BssT1I CCWWGG 3 cut(s) 233, 1443, 1491
Bst2UI CCWGG 5 cut(s) 162, 596, 1065, 1304, 1577
Bst4CI ACNGT 6 cut(s) 412, 499, 1041, 1136, 1435, 1724
Bst6I CTCTTC 2 cut(s) 763, 1323
BstACI GRCGYC 1 cut(s) 1292
BstAPI GCANNNNNTGC 1 cut(s) 86
BstC8I GCNNGC 5 cut(s) 82, 691, 876, 922, 1588
BstDEI CTNAG 4 cut(s) 752, 916, 1249, 1513
BstDSI CCRYGG 1 cut(s) 1491
BstEII GGTNACC 1 cut(s) 431
BstENI CCTNNNNNAGG 1 cut(s) 1233
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 976
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 2 cut(s) 140, 751
BstMBI GATC 2 cut(s) 137, 748
BstMWI GCNNNNNNNGC 4 cut(s) 86, 921, 982, 1289
BstNI CCWGG 5 cut(s) 162, 596, 1065, 1304, 1577
BstNSI RCATGY 2 cut(s) 1217, 1274
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 5 cut(s) 160, 594, 1063, 1302, 1575
BstSFI CTRYAG 1 cut(s) 75
BstUI CGCG 1 cut(s) 976
BstV1I GCAGC 1 cut(s) 89
BstV2I GAAGAC 1 cut(s) 1644
BstX2I RGATCY 1 cut(s) 748
BstXI CCANNNNNNTGG 2 cut(s) 734, 1196
BstYI RGATCY 1 cut(s) 748
BsuRI GGCC 4 cut(s) 924, 1165, 1200, 1426
BtgI CCRYGG 1 cut(s) 1491
BtsCI GGATG 1 cut(s) 451
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 2 cut(s) 182, 417
BveI ACCTGC 2 cut(s) 731, 852
Cac8I GCNNGC 5 cut(s) 82, 691, 876, 922, 1588
CfoI GCGC 1 cut(s) 900
Cfr13I GGNCC 3 cut(s) 701, 1467, 1625
CseI GACGC 2 cut(s) 921, 1281
Csp6I GTAC 2 cut(s) 312, 769
CviQI GTAC 2 cut(s) 312, 769
DdeI CTNAG 4 cut(s) 752, 916, 1249, 1513
DpnI GATC 2 cut(s) 139, 750
DpnII GATC 2 cut(s) 137, 748
DrdI GACNNNNNNGTC 1 cut(s) 657
DseDI GACNNNNNNGTC 1 cut(s) 657
EaeI YGGCCR 1 cut(s) 922
Eam1104I CTCTTC 2 cut(s) 763, 1323
EarI CTCTTC 2 cut(s) 763, 1323
Ecl136II GAGCTC 2 cut(s) 689, 1341
Eco130I CCWWGG 3 cut(s) 233, 1443, 1491
Eco24I GRGCYC 2 cut(s) 691, 1343
Eco32I GATATC 1 cut(s) 1010
Eco47I GGWCC 3 cut(s) 701, 1467, 1625
Eco53kI GAGCTC 2 cut(s) 689, 1341
Eco57I CTGAAG 4 cut(s) 971, 1137, 1203, 1576
Eco88I CYCGRG 1 cut(s) 1202
Eco91I GGTNACC 1 cut(s) 431
EcoICRI GAGCTC 2 cut(s) 689, 1341
EcoNI CCTNNNNNAGG 1 cut(s) 1233
EcoO109I RGGNCCY 1 cut(s) 1467
EcoO65I GGTNACC 1 cut(s) 431
EcoRI GAATTC 2 cut(s) 881, 1244
EcoRII CCWGG 5 cut(s) 160, 594, 1063, 1302, 1575
EcoRV GATATC 1 cut(s) 1010
EcoT14I CCWWGG 3 cut(s) 233, 1443, 1491
EcoT22I ATGCAT 2 cut(s) 91, 301
EcoT38I GRGCYC 2 cut(s) 691, 1343
ErhI CCWWGG 3 cut(s) 233, 1443, 1491
FalI AAGNNNNNCTT 2 cut(s) 520, 552
FauI CCCGC 1 cut(s) 1737
FblI GTMKAC 1 cut(s) 910
Fnu4HI GCNGC 2 cut(s) 78, 937
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 2 cut(s) 691, 1343
Fsp4HI GCNGC 2 cut(s) 78, 937
FspBI CTAG 6 cut(s) 326, 486, 588, 623, 953, 1533
GlaI GCGC 1 cut(s) 899
GluI GCNGC 2 cut(s) 78, 937
HaeIII GGCC 4 cut(s) 924, 1165, 1200, 1426
HgaI GACGC 2 cut(s) 921, 1281
HhaI GCGC 1 cut(s) 900
Hin1I GRCGYC 1 cut(s) 1292
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 4 cut(s) 662, 851, 1296, 1546
HindII GTYRAC 4 cut(s) 662, 851, 1296, 1546
HphI GGTGA 3 cut(s) 23, 554, 712
Hpy166II GTNNAC 6 cut(s) 662, 704, 851, 911, 1296, 1546
Hpy188I TCNGA 6 cut(s) 250, 669, 714, 1054, 1222, 1528
Hpy188III TCNNGA 7 cut(s) 141, 451, 1082, 1204, 1236, 1276, 1616
Hpy8I GTNNAC 6 cut(s) 662, 704, 851, 911, 1296, 1546
HpyAV CCTTC 5 cut(s) 245, 539, 1239, 1437, 1480
HpyCH4III ACNGT 6 cut(s) 412, 499, 1041, 1136, 1435, 1724
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 921, 982, 1289
HpyF3I CTNAG 4 cut(s) 752, 916, 1249, 1513
Hsp92I GRCGYC 1 cut(s) 1292
HspAI GCGC 1 cut(s) 898
Kzo9I GATC 2 cut(s) 137, 748
LguI GCTCTTC 1 cut(s) 763
LmnI GCTCC 5 cut(s) 229, 418, 563, 1647, 1672
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 4 cut(s) 286, 784, 1274, 1292
MaeI CTAG 6 cut(s) 326, 486, 588, 623, 953, 1533
MaeIII GTNAC 8 cut(s) 180, 197, 215, 431, 488, 740, 1144, 1595
MalI GATC 2 cut(s) 139, 750
MboI GATC 2 cut(s) 137, 748
MboII GAAGA 8 cut(s) 102, 105, 108, 750, 1097, 1130, 1310, 1644
MfeI CAATTG 2 cut(s) 291, 1646
MflI RGATCY 1 cut(s) 748
MhlI GDGCHC 3 cut(s) 691, 1312, 1343
MlsI TGGCCA 1 cut(s) 924
MluCI AATT 9 cut(s) 153, 190, 291, 352, 563, 881, 1244, 1413, 1646
MluNI TGGCCA 1 cut(s) 924
MlyI GAGTC 5 cut(s) 580, 593, 709, 1505, 1589
MmeI TCCRAC 2 cut(s) 1213, 1576
Mox20I TGGCCA 1 cut(s) 924
Mph1103I ATGCAT 2 cut(s) 91, 301
MscI TGGCCA 1 cut(s) 924
MseI TTAA 6 cut(s) 351, 825, 894, 1110, 1416, 1738
MslI CAYNNNNRTG 2 cut(s) 224, 1401
Msp20I TGGCCA 1 cut(s) 924
MspR9I CCNGG 5 cut(s) 162, 596, 1065, 1304, 1577
MunI CAATTG 2 cut(s) 291, 1646
Mva1269I GAATGC 2 cut(s) 320, 1071
MvaI CCWGG 5 cut(s) 162, 596, 1065, 1304, 1577
MvnI CGCG 1 cut(s) 976
MwoI GCNNNNNNNGC 4 cut(s) 86, 921, 982, 1289
NcoI CCATGG 1 cut(s) 1491
NdeII GATC 2 cut(s) 137, 748
NlaIV GGNNCC 4 cut(s) 231, 420, 559, 702
NmuCI GTSAC 4 cut(s) 180, 197, 1144, 1595
NsiI ATGCAT 2 cut(s) 91, 301
NspI RCATGY 2 cut(s) 1217, 1274
PaqCI CACCTGC 1 cut(s) 731
PciI ACATGT 1 cut(s) 1270
PciSI GCTCTTC 1 cut(s) 763
PctI GAATGC 2 cut(s) 320, 1071
PfeI GAWTC 8 cut(s) 71, 365, 482, 551, 599, 1088, 1124, 1529
PflMI CCANNNNNTGG 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 1575
PkrI GCNGC 2 cut(s) 79, 938
PleI GAGTC 5 cut(s) 579, 592, 709, 1505, 1588
PpsI GAGTC 5 cut(s) 579, 592, 709, 1505, 1588
PpuMI RGGWCCY 1 cut(s) 1467
PscI ACATGT 1 cut(s) 1270
PshBI ATTAAT 1 cut(s) 1416
Psp124BI GAGCTC 2 cut(s) 691, 1343
Psp5II RGGWCCY 1 cut(s) 1467
Psp6I CCWGG 5 cut(s) 160, 594, 1063, 1302, 1575
PspEI GGTNACC 1 cut(s) 431
PspGI CCWGG 5 cut(s) 160, 594, 1063, 1302, 1575
PspN4I GGNNCC 4 cut(s) 231, 420, 559, 702
PspPI GGNCC 3 cut(s) 701, 1467, 1625
PspPPI RGGWCCY 1 cut(s) 1467
PstI CTGCAG 1 cut(s) 79
PsuI RGATCY 1 cut(s) 748
RsaI GTAC 2 cut(s) 313, 770
RsaNI GTAC 2 cut(s) 312, 769
RseI CAYNNNNRTG 2 cut(s) 224, 1401
SacI GAGCTC 2 cut(s) 691, 1343
SapI GCTCTTC 1 cut(s) 763
SaqAI TTAA 6 cut(s) 351, 825, 894, 1110, 1416, 1738
SatI GCNGC 2 cut(s) 78, 937
Sau3AI GATC 2 cut(s) 137, 748
Sau96I GGNCC 3 cut(s) 701, 1467, 1625
SchI GAGTC 5 cut(s) 580, 593, 709, 1505, 1589
ScrFI CCNGG 5 cut(s) 162, 596, 1065, 1304, 1577
SduI GDGCHC 3 cut(s) 691, 1312, 1343
SfaNI GCATC 4 cut(s) 286, 784, 1274, 1292
SfcI CTRYAG 1 cut(s) 75
SinI GGWCC 3 cut(s) 701, 1467, 1625
SmiMI CAYNNNNRTG 2 cut(s) 224, 1401
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
Sse9I AATT 9 cut(s) 153, 190, 291, 352, 563, 881, 1244, 1413, 1646
SsiI CCGC 2 cut(s) 937, 1730
SspMI CTAG 6 cut(s) 326, 486, 588, 623, 953, 1533
SstI GAGCTC 2 cut(s) 691, 1343
StyD4I CCNGG 5 cut(s) 160, 594, 1063, 1302, 1575
StyI CCWWGG 3 cut(s) 233, 1443, 1491
TaaI ACNGT 6 cut(s) 412, 499, 1041, 1136, 1435, 1724
TaqI TCGA 3 cut(s) 357, 569, 885
TasI AATT 9 cut(s) 153, 190, 291, 352, 563, 881, 1244, 1413, 1646
TatI WGTACW 1 cut(s) 311
TauI GCSGC 1 cut(s) 939
TfiI GAWTC 8 cut(s) 71, 365, 482, 551, 599, 1088, 1124, 1529
Tru1I TTAA 6 cut(s) 351, 825, 894, 1110, 1416, 1738
Tru9I TTAA 6 cut(s) 351, 825, 894, 1110, 1416, 1738
TscAI CASTG 2 cut(s) 189, 417
TseFI GTSAC 4 cut(s) 180, 197, 1144, 1595
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 4 cut(s) 180, 197, 1144, 1595
TspDTI ATGAA 8 cut(s) 30, 376, 522, 591, 722, 857, 1137, 1434
TspRI CASTG 2 cut(s) 189, 417
Van91I CCANNNNNTGG 1 cut(s) 442
VpaK11BI GGWCC 3 cut(s) 701, 1467, 1625
VspI ATTAAT 1 cut(s) 1416
XagI CCTNNNNNAGG 1 cut(s) 1233
XapI RAATTY 4 cut(s) 153, 563, 881, 1244
XceI RCATGY 2 cut(s) 1217, 1274
XcmI CCANNNNNNNNNTGG 2 cut(s) 1283, 1403
XmiI GTMKAC 1 cut(s) 910
XspI CTAG 6 cut(s) 326, 486, 588, 623, 953, 1533
Zsp2I ATGCAT 2 cut(s) 91, 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.