Rh4BG367700

Squamosa promoter-binding-like protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005916
Physical Location & Seq
Forward (+)
52645402 .. 52648880
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG367700.1

Sequence Viewer

Length: 1728 bp
ATGAAGTTTTATTGCAAAGGCTCTGTTTGGTTGTTGAGGAAAACAAAAATGGAGTGGCAATCTGCAGCAGGCTATGCATTTTTCTTTGCTTGGTTTTCTTCTTCTTCTTTGTTCTTTACTGGGGGATCAAGAACCAGAGAAATTCAACCAGGCATTTCTGTATTAGTGTCACTGCATAATTGCTGTGACTTTTTGGTTCTGTGTTACATACTCATGGAGCCTTGGAGTTTCGGTTCAGAAGGGAAAGGGTTTTTACTTGCTGATGAGATGGATATACCAATTGATGCATTTGCAAGGAGTACAAAAGCATTCCTAGAATGGGATAACAAAACTTGCTTTAATTTCGATAAAGAATCAGTTGAGAGCATGGAGTTCATGGATTTGGGATTGCCTGATACAGTGAGGGAGCCTTGTCATGGTAACCAAGTAGTGGATGTCTTGAGTGCTGTCGCTGGTGTGGGCTGTAATGATTCTAGTAACAATACCGTAGCTTCTCCTACTTGTTTGATGACTTCAAGTTCATCTTGGGGTGAAGGAGAATCTGGCTCCAAATTTTCGAGTCCAGTCTGTGAGTCTAGTAGCCAGGATTCATCACTTATTGCTTTGAAGCTAGGGAGGTTGGCTGATAGCAAATATGGACAGGATAGTCAACATCTGAAAGAGAGGTCAAAGAGAGCTCGCACAAAGGGTCCACACTATCAGACTCTCACCTGCCAAGTTCATGGTTGTTACATGGATCTTAGCTCTTCAAAGGAGTACCATAAGAGGCATAGAGTTTGTGATGCTCACTCAAAGACTGCAATAGTTATCGTTAATGGCATTGAGCAGAGGTTTTGTCAACAATGTAGCAGGTTTCATCTGCTGGCTGAATTCGATGATGTTAAGCGCAGTTGTCGTAGACGCTTAGCTGGCCACAATCAACGCCGCAGGAAGCCTCAACTAGATACTTTTGATTGTAAATCGCGTAAGTGGCTTCAGTCATATCAAGGCATTGGATATCTTGGAACTTCCTTGCCAAAGAGAACACCGTTTGTCTTTTCAGATATACTTCCAGGTGGCATTCTATATCCAGAAGAATCTAACGAGTATGGACATATTAAAACTGAAGATGAATCAACTTACAGTCAATGTGTGACACCCATAACAAACAGGCTATTACCTCCAAACTCTTTCCTCCAACTGCATGGCCTCGGGAAGCAACATGCTTCAGAAGTTTCTTCATCAGGAAGTGAATTCTCGGTTTTTGATACTGCATCACATGTCCAGAAAGCATCTGGGGCGTCAACCTACCAGGGTGCTCTCTCTCTTCTGTCAGCTCAATCACAGAGCTCAATCCATTCAGCAAGAAATCAAGTGGCTACCCCTCTGCAAATACAGAGAATCCATCATGGTGCTGGTCAAATTAATGAACGGCCTTCCACAGTAAAAGCCTTGGAAAAATATGGACGAAACAGGTCCTTCCCATGCAGGATAAACTCCATGGGAGCTGACCAGATGGACTCAGTAATGATTTCTGATTCTAGTCATGCTGTTGACTTCCAACTTCACACAGATAGGGTTTTCCAGGAGTCAGCTCGCTTCAGTCACAAGTATCATCTTTCTCCTGAAAATGGACCAACTTTTGATTTGCTCCAATTGTCTTCACATTTTCAAAGAGTGGAGCAAGAGAGAAACTTTTTGCAAGTCAAACATGAGCAGGGGGATTTCTACTGTTTCCCGCAATCTTAA

Protein Analysis

575

Amino Acids

64.55

Weight (kDa)

7.82

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 238 - 311 2.9e-31 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 719
AasI GACNNNNNNGTC 1 cut(s) 645
Acc36I ACCTGC 2 cut(s) 719, 840
AccB7I CCANNNNNTGG 1 cut(s) 430
AccI GTMKAC 1 cut(s) 898
AccII CGCG 1 cut(s) 964
AciI CCGC 2 cut(s) 925, 1718
AclWI GGATC 2 cut(s) 133, 744
AcoI YGGCCR 1 cut(s) 910
AcsI RAATTY 4 cut(s) 141, 551, 869, 1232
AcuI CTGAAG 4 cut(s) 959, 1125, 1191, 1564
AcyI GRCGYC 1 cut(s) 1280
AfaI GTAC 2 cut(s) 301, 758
AfiI CCNNNNNNNGG 4 cut(s) 319, 416, 430, 1610
AflIII ACRYGT 1 cut(s) 1258
AgsI TTSAA 5 cut(s) 146, 516, 607, 750, 1652
AjnI CCWGG 5 cut(s) 148, 582, 1051, 1290, 1563
AloI GAACNNNNNNTCC 2 cut(s) 217, 249
AluBI AGCT 9 cut(s) 491, 610, 677, 744, 908, 1316, 1329, 1487, 1574
AluI AGCT 9 cut(s) 491, 610, 677, 744, 908, 1316, 1329, 1487, 1574
Alw21I GWGCWC 3 cut(s) 679, 1300, 1331
AlwI GGATC 2 cut(s) 133, 744
Ama87I CYCGRG 1 cut(s) 1190
AoxI GGCC 3 cut(s) 910, 1186, 1412
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 4 cut(s) 141, 551, 869, 1232
AseI ATTAAT 1 cut(s) 1404
AspLEI GCGC 1 cut(s) 888
AspS9I GGNCC 3 cut(s) 689, 1455, 1613
AsuHPI GGTGA 2 cut(s) 542, 700
AvaI CYCGRG 1 cut(s) 1190
AvaII GGWCC 3 cut(s) 689, 1455, 1613
BalI TGGCCA 1 cut(s) 912
BanII GRGCYC 2 cut(s) 679, 1331
BbsI GAAGAC 1 cut(s) 1632
Bbv12I GWGCWC 3 cut(s) 679, 1300, 1331
BbvI GCAGC 1 cut(s) 77
BccI CCATC 3 cut(s) 262, 1392, 1489
BceAI ACGGC 1 cut(s) 1427
BciT130I CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
BfaI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
BfmI CTRYAG 1 cut(s) 63
BfuAI ACCTGC 2 cut(s) 719, 840
BisI GCNGC 2 cut(s) 66, 925
BlpI GCTNAGC 1 cut(s) 904
BlsI GCNGC 2 cut(s) 67, 926
Bme1390I CCNGG 5 cut(s) 150, 584, 1053, 1292, 1565
Bme18I GGWCC 3 cut(s) 689, 1455, 1613
BmeT110I CYCGRG 1 cut(s) 1190
BmgT120I GGNCC 3 cut(s) 689, 1455, 1613
BmiI GGNNCC 4 cut(s) 219, 408, 547, 690
BmrFI CCNGG 5 cut(s) 150, 584, 1053, 1292, 1565
BmrI ACTGGG 1 cut(s) 129
BmsI GCATC 4 cut(s) 274, 772, 1262, 1280
BmuI ACTGGG 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 1632
Bpu1102I GCTNAGC 1 cut(s) 904
BpuEI CTTGAG 1 cut(s) 460
BsaHI GRCGYC 1 cut(s) 1280
BsaJI CCNNGG 5 cut(s) 221, 1189, 1291, 1431, 1479
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 416, 430, 1610
Bse1I ACTGG 2 cut(s) 124, 563
BseBI CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
BseDI CCNNGG 5 cut(s) 221, 1189, 1291, 1431, 1479
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 4 cut(s) 319, 416, 430, 1610
BseMII CTCAG 1 cut(s) 1515
BseNI ACTGG 2 cut(s) 124, 563
BseXI GCAGC 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 964
BshFI GGCC 3 cut(s) 912, 1188, 1414
BsiHKAI GWGCWC 3 cut(s) 679, 1300, 1331
BsiHKCI CYCGRG 1 cut(s) 1190
BslI CCNNNNNNNGG 4 cut(s) 319, 416, 430, 1610
BsmI GAATGC 2 cut(s) 308, 1059
BsnI GGCC 3 cut(s) 912, 1188, 1414
BsoBI CYCGRG 1 cut(s) 1190
Bsp1286I GDGCHC 3 cut(s) 679, 1300, 1331
Bsp143I GATC 2 cut(s) 125, 736
Bsp1720I GCTNAGC 1 cut(s) 904
Bsp19I CCATGG 1 cut(s) 1479
BspACI CCGC 2 cut(s) 925, 1718
BspANI GGCC 3 cut(s) 912, 1188, 1414
BspCNI CTCAG 1 cut(s) 1514
BspFNI CGCG 1 cut(s) 964
BspLI GGNNCC 4 cut(s) 219, 408, 547, 690
BspMAI CTGCAG 1 cut(s) 67
BspMI ACCTGC 2 cut(s) 719, 840
BspPI GGATC 2 cut(s) 133, 744
BspQI GCTCTTC 1 cut(s) 751
BsrI ACTGG 2 cut(s) 124, 563
BssECI CCNNGG 5 cut(s) 221, 1189, 1291, 1431, 1479
BssMI GATC 2 cut(s) 125, 736
BssNI GRCGYC 1 cut(s) 1280
BssT1I CCWWGG 3 cut(s) 221, 1431, 1479
Bst2UI CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
Bst4CI ACNGT 6 cut(s) 400, 487, 1029, 1124, 1423, 1712
Bst6I CTCTTC 2 cut(s) 751, 1311
BstACI GRCGYC 1 cut(s) 1280
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 5 cut(s) 70, 679, 864, 910, 1576
BstDEI CTNAG 3 cut(s) 740, 904, 1501
BstDSI CCRYGG 1 cut(s) 1479
BstEII GGTNACC 1 cut(s) 419
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 1 cut(s) 964
BstHHI GCGC 1 cut(s) 888
BstKTI GATC 2 cut(s) 128, 739
BstMBI GATC 2 cut(s) 125, 736
BstMWI GCNNNNNNNGC 4 cut(s) 74, 909, 970, 1277
BstNI CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
BstNSI RCATGY 2 cut(s) 1205, 1262
BstPI GGTNACC 1 cut(s) 419
BstSCI CCNGG 5 cut(s) 148, 582, 1051, 1290, 1563
BstSFI CTRYAG 1 cut(s) 63
BstUI CGCG 1 cut(s) 964
BstV1I GCAGC 1 cut(s) 77
BstV2I GAAGAC 1 cut(s) 1632
BstX2I RGATCY 1 cut(s) 736
BstXI CCANNNNNNTGG 2 cut(s) 722, 1184
BstYI RGATCY 1 cut(s) 736
BsuRI GGCC 3 cut(s) 912, 1188, 1414
BtgI CCRYGG 1 cut(s) 1479
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 170, 405
BveI ACCTGC 2 cut(s) 719, 840
Cac8I GCNNGC 5 cut(s) 70, 679, 864, 910, 1576
CfoI GCGC 1 cut(s) 888
Cfr13I GGNCC 3 cut(s) 689, 1455, 1613
CseI GACGC 2 cut(s) 909, 1269
Csp6I GTAC 2 cut(s) 300, 757
CviQI GTAC 2 cut(s) 300, 757
DdeI CTNAG 3 cut(s) 740, 904, 1501
DpnI GATC 2 cut(s) 127, 738
DpnII GATC 2 cut(s) 125, 736
DrdI GACNNNNNNGTC 1 cut(s) 645
DseDI GACNNNNNNGTC 1 cut(s) 645
EaeI YGGCCR 1 cut(s) 910
Eam1104I CTCTTC 2 cut(s) 751, 1311
EarI CTCTTC 2 cut(s) 751, 1311
Ecl136II GAGCTC 2 cut(s) 677, 1329
Eco130I CCWWGG 3 cut(s) 221, 1431, 1479
Eco24I GRGCYC 2 cut(s) 679, 1331
Eco32I GATATC 1 cut(s) 998
Eco47I GGWCC 3 cut(s) 689, 1455, 1613
Eco53kI GAGCTC 2 cut(s) 677, 1329
Eco57I CTGAAG 4 cut(s) 959, 1125, 1191, 1564
Eco88I CYCGRG 1 cut(s) 1190
Eco91I GGTNACC 1 cut(s) 419
EcoICRI GAGCTC 2 cut(s) 677, 1329
EcoO109I RGGNCCY 1 cut(s) 1455
EcoO65I GGTNACC 1 cut(s) 419
EcoRI GAATTC 2 cut(s) 869, 1232
EcoRII CCWGG 5 cut(s) 148, 582, 1051, 1290, 1563
EcoRV GATATC 1 cut(s) 998
EcoT14I CCWWGG 3 cut(s) 221, 1431, 1479
EcoT22I ATGCAT 2 cut(s) 79, 289
EcoT38I GRGCYC 2 cut(s) 679, 1331
ErhI CCWWGG 3 cut(s) 221, 1431, 1479
FalI AAGNNNNNCTT 2 cut(s) 508, 540
FauI CCCGC 1 cut(s) 1725
FblI GTMKAC 1 cut(s) 898
Fnu4HI GCNGC 2 cut(s) 66, 925
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 2 cut(s) 679, 1331
Fsp4HI GCNGC 2 cut(s) 66, 925
FspBI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
GlaI GCGC 1 cut(s) 887
GluI GCNGC 2 cut(s) 66, 925
HaeIII GGCC 3 cut(s) 912, 1188, 1414
HgaI GACGC 2 cut(s) 909, 1269
HhaI GCGC 1 cut(s) 888
Hin1I GRCGYC 1 cut(s) 1280
Hin6I GCGC 1 cut(s) 886
HinP1I GCGC 1 cut(s) 886
HincII GTYRAC 4 cut(s) 650, 839, 1284, 1534
HindII GTYRAC 4 cut(s) 650, 839, 1284, 1534
HphI GGTGA 2 cut(s) 542, 700
Hpy166II GTNNAC 6 cut(s) 650, 692, 839, 899, 1284, 1534
Hpy188I TCNGA 6 cut(s) 238, 657, 702, 1042, 1210, 1516
Hpy188III TCNNGA 7 cut(s) 129, 439, 1070, 1192, 1224, 1264, 1604
Hpy8I GTNNAC 6 cut(s) 650, 692, 839, 899, 1284, 1534
HpyAV CCTTC 4 cut(s) 233, 527, 1425, 1468
HpyCH4III ACNGT 6 cut(s) 400, 487, 1029, 1124, 1423, 1712
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 909, 970, 1277
HpyF3I CTNAG 3 cut(s) 740, 904, 1501
Hsp92I GRCGYC 1 cut(s) 1280
HspAI GCGC 1 cut(s) 886
Kzo9I GATC 2 cut(s) 125, 736
LguI GCTCTTC 1 cut(s) 751
LmnI GCTCC 6 cut(s) 217, 406, 551, 1484, 1635, 1660
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 4 cut(s) 274, 772, 1262, 1280
MaeI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
MaeIII GTNAC 8 cut(s) 168, 185, 203, 419, 476, 728, 1132, 1583
MalI GATC 2 cut(s) 127, 738
MboI GATC 2 cut(s) 125, 736
MboII GAAGA 9 cut(s) 90, 93, 96, 738, 1085, 1118, 1209, 1298, 1632
MfeI CAATTG 2 cut(s) 279, 1634
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 3 cut(s) 679, 1300, 1331
MlsI TGGCCA 1 cut(s) 912
MluCI AATT 9 cut(s) 141, 178, 279, 340, 551, 869, 1232, 1401, 1634
MluNI TGGCCA 1 cut(s) 912
MlyI GAGTC 5 cut(s) 568, 581, 697, 1493, 1577
MmeI TCCRAC 2 cut(s) 1201, 1564
Mox20I TGGCCA 1 cut(s) 912
Mph1103I ATGCAT 2 cut(s) 79, 289
MscI TGGCCA 1 cut(s) 912
MseI TTAA 6 cut(s) 339, 813, 882, 1098, 1404, 1726
MslI CAYNNNNRTG 2 cut(s) 212, 1389
Msp20I TGGCCA 1 cut(s) 912
MspR9I CCNGG 5 cut(s) 150, 584, 1053, 1292, 1565
MunI CAATTG 2 cut(s) 279, 1634
Mva1269I GAATGC 2 cut(s) 308, 1059
MvaI CCWGG 5 cut(s) 150, 584, 1053, 1292, 1565
MvnI CGCG 1 cut(s) 964
MwoI GCNNNNNNNGC 4 cut(s) 74, 909, 970, 1277
NcoI CCATGG 1 cut(s) 1479
NdeII GATC 2 cut(s) 125, 736
NlaIV GGNNCC 4 cut(s) 219, 408, 547, 690
NmuCI GTSAC 4 cut(s) 168, 185, 1132, 1583
NsiI ATGCAT 2 cut(s) 79, 289
NspI RCATGY 2 cut(s) 1205, 1262
PaqCI CACCTGC 1 cut(s) 719
PciI ACATGT 1 cut(s) 1258
PciSI GCTCTTC 1 cut(s) 751
PctI GAATGC 2 cut(s) 308, 1059
PfeI GAWTC 8 cut(s) 353, 470, 539, 587, 1076, 1112, 1380, 1517
PflMI CCANNNNNTGG 1 cut(s) 430
PfoI TCCNGGA 1 cut(s) 1563
PkrI GCNGC 2 cut(s) 67, 926
PleI GAGTC 5 cut(s) 567, 580, 697, 1493, 1576
PpsI GAGTC 5 cut(s) 567, 580, 697, 1493, 1576
PpuMI RGGWCCY 1 cut(s) 1455
PscI ACATGT 1 cut(s) 1258
PshBI ATTAAT 1 cut(s) 1404
Psp124BI GAGCTC 2 cut(s) 679, 1331
Psp5II RGGWCCY 1 cut(s) 1455
Psp6I CCWGG 5 cut(s) 148, 582, 1051, 1290, 1563
PspEI GGTNACC 1 cut(s) 419
PspGI CCWGG 5 cut(s) 148, 582, 1051, 1290, 1563
PspN4I GGNNCC 4 cut(s) 219, 408, 547, 690
PspPI GGNCC 3 cut(s) 689, 1455, 1613
PspPPI RGGWCCY 1 cut(s) 1455
PstI CTGCAG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 736
RsaI GTAC 2 cut(s) 301, 758
RsaNI GTAC 2 cut(s) 300, 757
RseI CAYNNNNRTG 2 cut(s) 212, 1389
SacI GAGCTC 2 cut(s) 679, 1331
SapI GCTCTTC 1 cut(s) 751
SaqAI TTAA 6 cut(s) 339, 813, 882, 1098, 1404, 1726
SatI GCNGC 2 cut(s) 66, 925
Sau3AI GATC 2 cut(s) 125, 736
Sau96I GGNCC 3 cut(s) 689, 1455, 1613
SchI GAGTC 5 cut(s) 568, 581, 697, 1493, 1577
ScrFI CCNGG 5 cut(s) 150, 584, 1053, 1292, 1565
SduI GDGCHC 3 cut(s) 679, 1300, 1331
SfaNI GCATC 4 cut(s) 274, 772, 1262, 1280
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 3 cut(s) 689, 1455, 1613
SmiMI CAYNNNNRTG 2 cut(s) 212, 1389
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 9 cut(s) 141, 178, 279, 340, 551, 869, 1232, 1401, 1634
SsiI CCGC 2 cut(s) 925, 1718
SspMI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
SstI GAGCTC 2 cut(s) 679, 1331
StyD4I CCNGG 5 cut(s) 148, 582, 1051, 1290, 1563
StyI CCWWGG 3 cut(s) 221, 1431, 1479
TaaI ACNGT 6 cut(s) 400, 487, 1029, 1124, 1423, 1712
TaqI TCGA 3 cut(s) 345, 557, 873
TasI AATT 9 cut(s) 141, 178, 279, 340, 551, 869, 1232, 1401, 1634
TatI WGTACW 1 cut(s) 299
TauI GCSGC 1 cut(s) 927
TfiI GAWTC 8 cut(s) 353, 470, 539, 587, 1076, 1112, 1380, 1517
Tru1I TTAA 6 cut(s) 339, 813, 882, 1098, 1404, 1726
Tru9I TTAA 6 cut(s) 339, 813, 882, 1098, 1404, 1726
TscAI CASTG 2 cut(s) 177, 405
TseFI GTSAC 4 cut(s) 168, 185, 1132, 1583
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 4 cut(s) 168, 185, 1132, 1583
TspDTI ATGAA 9 cut(s) 17, 364, 510, 579, 710, 845, 1125, 1209, 1422
TspRI CASTG 2 cut(s) 177, 405
Van91I CCANNNNNTGG 1 cut(s) 430
VpaK11BI GGWCC 3 cut(s) 689, 1455, 1613
VspI ATTAAT 1 cut(s) 1404
XapI RAATTY 4 cut(s) 141, 551, 869, 1232
XceI RCATGY 2 cut(s) 1205, 1262
XcmI CCANNNNNNNNNTGG 2 cut(s) 1271, 1391
XmiI GTMKAC 1 cut(s) 898
XspI CTAG 6 cut(s) 314, 474, 576, 611, 941, 1521
Zsp2I ATGCAT 2 cut(s) 79, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.