MD13G1059500.v1.1

Squamosa promoter-binding-like protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005916
Physical Location & Seq
Forward (+)
4177916 .. 4182525
4610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1059500.v1.1.491

Sequence Viewer

Length: 1650 bp
ATGGAGTCTTGGAGTTTTGGTTCAGAAGGGAAAGGGTTTTTGTTTCCGGAAGAAATGGATATACCAACTGATGCATTTTCAAGGAGTACTAGGAAAGCATTGCTAGAATTGGATAACAAAACTTGCTTTAATTTTGGTAAGGAATCAGTTGATAGCGTGGGGTTCATGGATTTGGGATTCCCTGATATTAACCAAGGAGTGGAAATCTTGAATGGTGTGATTGGTAACGATTCTAGTTATAATTCTGTAGCTTCACCTTGTTGTTTGATTAGTTCAAGTTCATCTTTTGGTGATGGGGAATCTGGGTCGAAGTTTTCGAGCCCTGTGACTGAATCTAATAGCATGGATTCATCAATGATTGCTTTGAAGCTAGGGGGGTTTGCTGATAGCAGAGGTGGGCAGAACAGTCAGCATTCGATTTCTAGAGAGAGGTCGAAAAGAGCTGGCAATGGCATGAGGAGTTCATGCTATCATGCTCCAACCTGCCAAGTCCATGGTTGTAACATGGATCTTACCTTTTCAAAGGATTACCACAAGAGGCATAGGGTTTGTGATGCTCACTCCAAGACTGCCGTAGTGATTGTTAATGGAATCAAACAGCGGTTTTGCCAGCAGTGCAGCAGGTTTCATCTTCTTGCTGAATTTGATGATGTGAAGCGCAGTTGTCGTAGACGCCTAGCTGGCCACAACCTTCGCCGAAGGAAGCCTCAACTAGATACCCTCGATTGTAAACCGCATAAGTTGATTCGGTCATGTGAAGGAAACGGGTATATGGGAACTTCCTTTTCGAAGGGAACACCCTTTGTTTTTTCGGATATACTTCCAGATGGCTGCATCCTGTATCCAGAATATGAAGAAGCTAACCTATATGGACATGTCAAATCTGAAGACAGGTCTACCTACGGCCAGTCTGTAACACCTATACCAAACAGGCAGTTTATTCCAAAATCATTCCGCCATCTGCCTGGCCTTGGAGAACAGCATGCTCCGGGAATTCTCTCGTCAGGAAATGAATACTCTGTCTTTGATACAGCATCAACCGCTGAGAAAGCATTTGAGACTCCATACTCGAGCTGTGCTCTCTCTCTTCTGTCAGCTCAATCGCATAACTTATCAAGCAATTCAGCCGGAGTTCAAGTGCCTAGCCCCCTGATAAATCAAAGAGCCCATCGCAGTTTTGGTCAACTTAATGAAAAGACTTTGAGGGTAAACTCCATGGAAAATTGTGGACGAAACAGGTCCTACCCGTTTAGTATGAAATCTATGGGAGCCTATCATATGGAATCAATCAAAATTTCTGATTCTAGTCATGCTGCTGACTTCCAACTTCACACAGACAGGGCTTTGCATGTGTCAGATTGCTTAAGTGACAAGTATTGTGTTTCTCCTGAACGCGGAACAACTTTTGATTTGCGGCAATTGTCATCACACCTTCAAAGAGTGGAGCATGAGAGAAACTTTTTTCAAGTAAAGCAGGAAAATGGAGAATTCTACTGTTTCCCGACGGGCGTGCAAGCAACAATGTTGCTGGAGGCTGGAGGCAGTTTGGATCTTCAGCAGAATTCCAACATTTCCATACAGAGGCTGGTAGAAACTTTAGATACCATTGGCAGTAACACTCTATTCCGAAAGGAAAATATAGCTATCTAG

Protein Analysis

550

Amino Acids

60.86

Weight (kDa)

7.03

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 162 - 235 9.1e-31 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
Acc36I ACCTGC 2 cut(s) 491, 612
AccB7I CCANNNNNTGG 1 cut(s) 199
AccI GTMKAC 2 cut(s) 670, 896
AccII CGCG 1 cut(s) 1395
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 6 cut(s) 601, 734, 955, 1041, 1395, 1414
AclWI GGATC 2 cut(s) 516, 1557
AcoI YGGCCR 2 cut(s) 682, 904
AcsI RAATTY 5 cut(s) 641, 993, 1293, 1487, 1561
AcuI CTGAAG 2 cut(s) 906, 1538
AcyI GRCGYC 1 cut(s) 673
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 4 cut(s) 199, 971, 1394, 1581
AflII CTTAAG 1 cut(s) 1363
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 8 cut(s) 81, 211, 276, 367, 522, 1136, 1436, 1466
AjnI CCWGG 1 cut(s) 964
AloI GAACNNNNNNTCC 1 cut(s) 36
AluBI AGCT 8 cut(s) 251, 370, 443, 680, 860, 1074, 1097, 1643
AluI AGCT 8 cut(s) 251, 370, 443, 680, 860, 1074, 1097, 1643
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 1 cut(s) 1052
AlwI GGATC 2 cut(s) 516, 1557
AlwNI CAGNNNCTG 1 cut(s) 1585
Ama87I CYCGRG 1 cut(s) 1069
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 3 cut(s) 682, 904, 967
ApeKI GCWGC 3 cut(s) 618, 831, 1313
ApoI RAATTY 5 cut(s) 641, 993, 1293, 1487, 1561
AspLEI GCGC 1 cut(s) 660
AspS9I GGNCC 1 cut(s) 1239
AsuC2I CCSGG 1 cut(s) 990
AsuHPI GGTGA 2 cut(s) 246, 302
AsuII TTCGAA 1 cut(s) 788
AvaI CYCGRG 1 cut(s) 1069
AvaII GGWCC 1 cut(s) 1239
BalI TGGCCA 1 cut(s) 684
BanII GRGCYC 2 cut(s) 323, 1168
BbsI GAAGAC 1 cut(s) 894
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 3 cut(s) 630, 818, 1300
BccI CCATC 4 cut(s) 287, 821, 966, 1176
BceAI ACGGC 2 cut(s) 557, 919
BcgI CGANNNNNNTGC 2 cut(s) 1492, 1526
BciT130I CCWGG 1 cut(s) 966
BciVI GTATCC 1 cut(s) 852
BcnI CCSGG 1 cut(s) 990
BcoDI GTCTC 1 cut(s) 1052
BfmI CTRYAG 1 cut(s) 246
BfrI CTTAAG 1 cut(s) 1363
BfuAI ACCTGC 2 cut(s) 491, 612
BfuI GTATCC 1 cut(s) 852
BglI GCCNNNNNGGC 1 cut(s) 681
BisI GCNGC 4 cut(s) 619, 832, 1314, 1415
BlsI GCNGC 4 cut(s) 620, 833, 1315, 1416
BmcAI AGTACT 1 cut(s) 88
Bme1390I CCNGG 2 cut(s) 966, 990
Bme18I GGWCC 1 cut(s) 1239
BmeT110I CYCGRG 1 cut(s) 1069
BmgT120I GGNCC 1 cut(s) 1239
BmiI GGNNCC 1 cut(s) 1270
BmrFI CCNGG 2 cut(s) 966, 990
BmsI GCATC 4 cut(s) 61, 544, 843, 1043
BpiI GAAGAC 1 cut(s) 894
BplI GAGNNNNNCTC 2 cut(s) 1063, 1095
BpmI CTGGAG 2 cut(s) 1550, 1557
Bpu14I TTCGAA 1 cut(s) 788
BpuMI CCSGG 1 cut(s) 990
BsaHI GRCGYC 1 cut(s) 673
BsaJI CCNNGG 4 cut(s) 193, 493, 970, 1215
BsaWI WCCGGW 1 cut(s) 46
BsaXI ACNNNNNCTCC 3 cut(s) 34, 1122, 1152
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 971, 1394, 1581
Bse1I ACTGG 1 cut(s) 907
Bse3DI GCAATG 2 cut(s) 98, 454
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 1 cut(s) 966
BseDI CCNNGG 4 cut(s) 193, 493, 970, 1215
BseGI GGATG 1 cut(s) 834
BseLI CCNNNNNNNGG 4 cut(s) 199, 971, 1394, 1581
BseMI GCAATG 2 cut(s) 98, 454
BseMII CTCAG 1 cut(s) 1035
BseNI ACTGG 1 cut(s) 907
BseRI GAGGAG 1 cut(s) 472
BseXI GCAGC 3 cut(s) 630, 818, 1300
BsgI GTGCAG 1 cut(s) 637
Bsh1236I CGCG 1 cut(s) 1395
BshFI GGCC 3 cut(s) 684, 906, 969
BsiHKAI GWGCWC 1 cut(s) 1081
BsiHKCI CYCGRG 1 cut(s) 1069
BsiSI CCGG 3 cut(s) 47, 989, 1128
BslI CCNNNNNNNGG 4 cut(s) 199, 971, 1394, 1581
BsmAI GTCTC 1 cut(s) 1052
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 3 cut(s) 684, 906, 969
BsoBI CYCGRG 1 cut(s) 1069
Bsp119I TTCGAA 1 cut(s) 788
Bsp1286I GDGCHC 3 cut(s) 323, 1081, 1168
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 2 cut(s) 508, 1549
Bsp19I CCATGG 2 cut(s) 493, 1215
BspACI CCGC 6 cut(s) 601, 734, 955, 1041, 1395, 1414
BspANI GGCC 3 cut(s) 684, 906, 969
BspCNI CTCAG 1 cut(s) 1036
BspEI TCCGGA 1 cut(s) 46
BspFNI CGCG 1 cut(s) 1395
BspLI GGNNCC 1 cut(s) 1270
BspMI ACCTGC 2 cut(s) 491, 612
BspPI GGATC 2 cut(s) 516, 1557
BspT104I TTCGAA 1 cut(s) 788
BspTI CTTAAG 1 cut(s) 1363
BsrDI GCAATG 2 cut(s) 98, 454
BsrI ACTGG 1 cut(s) 907
BssECI CCNNGG 4 cut(s) 193, 493, 970, 1215
BssMI GATC 2 cut(s) 508, 1549
BssNI GRCGYC 1 cut(s) 673
BssT1I CCWWGG 4 cut(s) 193, 493, 970, 1215
Bst2UI CCWGG 1 cut(s) 966
Bst4CI ACNGT 2 cut(s) 407, 1496
Bst6I CTCTTC 1 cut(s) 1092
BstACI GRCGYC 1 cut(s) 673
BstAFI CTTAAG 1 cut(s) 1363
BstBI TTCGAA 1 cut(s) 788
BstC8I GCNNGC 6 cut(s) 445, 611, 682, 984, 1511, 1515
BstDEI CTNAG 1 cut(s) 1044
BstDSI CCRYGG 2 cut(s) 493, 1215
BstF5I GGATG 1 cut(s) 834
BstFNI CGCG 1 cut(s) 1395
BstHHI GCGC 1 cut(s) 660
BstKTI GATC 2 cut(s) 511, 1552
BstMAI GTCTC 1 cut(s) 1052
BstMBI GATC 2 cut(s) 508, 1549
BstMWI GCNNNNNNNGC 4 cut(s) 615, 681, 1040, 1049
BstNI CCWGG 1 cut(s) 966
BstNSI RCATGY 3 cut(s) 878, 986, 1352
BstSCI CCNGG 2 cut(s) 964, 988
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 1 cut(s) 1395
BstV1I GCAGC 3 cut(s) 630, 818, 1300
BstV2I GAAGAC 1 cut(s) 894
BstX2I RGATCY 2 cut(s) 508, 1549
BstXI CCANNNNNNTGG 2 cut(s) 494, 965
BstYI RGATCY 2 cut(s) 508, 1549
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 3 cut(s) 684, 906, 969
BtgI CCRYGG 2 cut(s) 493, 1215
BtgZI GCGATG 1 cut(s) 1154
BtsCI GGATG 1 cut(s) 834
BtsI GCAGTG 1 cut(s) 620
BtsIMutI CAGTG 1 cut(s) 620
BveI ACCTGC 2 cut(s) 491, 612
Cac8I GCNNGC 6 cut(s) 445, 611, 682, 984, 1511, 1515
CaiI CAGNNNCTG 1 cut(s) 1585
CfoI GCGC 1 cut(s) 660
Cfr13I GGNCC 1 cut(s) 1239
CseI GACGC 1 cut(s) 681
Csp6I GTAC 1 cut(s) 87
CviQI GTAC 1 cut(s) 87
DdeI CTNAG 1 cut(s) 1044
DpnI GATC 2 cut(s) 510, 1551
DpnII GATC 2 cut(s) 508, 1549
EaeI YGGCCR 2 cut(s) 682, 904
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
EciI GGCGGA 1 cut(s) 944
Eco130I CCWWGG 4 cut(s) 193, 493, 970, 1215
Eco24I GRGCYC 2 cut(s) 323, 1168
Eco47I GGWCC 1 cut(s) 1239
Eco57I CTGAAG 2 cut(s) 906, 1538
Eco88I CYCGRG 1 cut(s) 1069
EcoO109I RGGNCCY 1 cut(s) 1239
EcoRI GAATTC 3 cut(s) 993, 1487, 1561
EcoRII CCWGG 1 cut(s) 964
EcoT14I CCWWGG 4 cut(s) 193, 493, 970, 1215
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 2 cut(s) 323, 1168
ErhI CCWWGG 4 cut(s) 193, 493, 970, 1215
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FauNDI CATATG 1 cut(s) 1278
FblI GTMKAC 2 cut(s) 670, 896
Fnu4HI GCNGC 4 cut(s) 619, 832, 1314, 1415
FokI GGATG 1 cut(s) 821
FriOI GRGCYC 2 cut(s) 323, 1168
Fsp4HI GCNGC 4 cut(s) 619, 832, 1314, 1415
GlaI GCGC 1 cut(s) 659
GluI GCNGC 4 cut(s) 619, 832, 1314, 1415
GsuI CTGGAG 2 cut(s) 1550, 1557
HaeIII GGCC 3 cut(s) 684, 906, 969
HapII CCGG 3 cut(s) 47, 989, 1128
HgaI GACGC 1 cut(s) 681
HhaI GCGC 1 cut(s) 660
Hin1I GRCGYC 1 cut(s) 673
Hin6I GCGC 1 cut(s) 658
HinP1I GCGC 1 cut(s) 658
HincII GTYRAC 1 cut(s) 1184
HindII GTYRAC 1 cut(s) 1184
HpaII CCGG 3 cut(s) 47, 989, 1128
HphI GGTGA 2 cut(s) 246, 302
Hpy166II GTNNAC 6 cut(s) 671, 731, 897, 1184, 1210, 1229
Hpy188I TCNGA 6 cut(s) 25, 814, 886, 1300, 1357, 1628
Hpy188III TCNNGA 8 cut(s) 47, 208, 423, 824, 845, 1005, 1388, 1501
Hpy8I GTNNAC 6 cut(s) 671, 731, 897, 1184, 1210, 1229
Hpy99I CGWCG 1 cut(s) 1507
HpyAV CCTTC 6 cut(s) 20, 693, 701, 752, 784, 1442
HpyCH4III ACNGT 2 cut(s) 407, 1496
HpyCH4V TGCA 5 cut(s) 74, 618, 834, 1348, 1513
HpyF10VI GCNNNNNNNGC 4 cut(s) 615, 681, 1040, 1049
HpyF3I CTNAG 1 cut(s) 1044
Hsp92I GRCGYC 1 cut(s) 673
HspAI GCGC 1 cut(s) 658
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 2 cut(s) 508, 1549
LmnI GCTCC 4 cut(s) 481, 991, 1268, 1444
Lsp1109I GCAGC 3 cut(s) 630, 818, 1300
LweI GCATC 4 cut(s) 61, 544, 843, 1043
MaeIII GTNAC 6 cut(s) 224, 325, 500, 913, 1367, 1613
MalI GATC 2 cut(s) 510, 1551
MboI GATC 2 cut(s) 508, 1549
MboII GAAGA 6 cut(s) 62, 623, 866, 899, 1079, 1544
MfeI CAATTG 1 cut(s) 1418
MflI RGATCY 2 cut(s) 508, 1549
MhlI GDGCHC 3 cut(s) 323, 1081, 1168
MlsI TGGCCA 1 cut(s) 684
MluNI TGGCCA 1 cut(s) 684
MlyI GAGTC 2 cut(s) 14, 1054
MmeI TCCRAC 3 cut(s) 503, 1348, 1590
Mox20I TGGCCA 1 cut(s) 684
Mph1103I ATGCAT 1 cut(s) 76
MroI TCCGGA 1 cut(s) 46
MscI TGGCCA 1 cut(s) 684
MseI TTAA 5 cut(s) 129, 189, 585, 1188, 1364
Msp20I TGGCCA 1 cut(s) 684
MspA1I CMGCKG 2 cut(s) 601, 1043
MspCI CTTAAG 1 cut(s) 1363
MspI CCGG 3 cut(s) 47, 989, 1128
MspR9I CCNGG 2 cut(s) 966, 990
MunI CAATTG 1 cut(s) 1418
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 1 cut(s) 966
MvnI CGCG 1 cut(s) 1395
MwoI GCNNNNNNNGC 4 cut(s) 615, 681, 1040, 1049
NciI CCSGG 1 cut(s) 990
NcoI CCATGG 2 cut(s) 493, 1215
NdeI CATATG 1 cut(s) 1278
NdeII GATC 2 cut(s) 508, 1549
NlaIV GGNNCC 1 cut(s) 1270
NmuCI GTSAC 2 cut(s) 325, 1367
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 3 cut(s) 878, 986, 1352
NspV TTCGAA 1 cut(s) 788
PaeI GCATGC 1 cut(s) 986
PaeR7I CTCGAG 1 cut(s) 1069
PciI ACATGT 1 cut(s) 874
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PctI GAATGC 1 cut(s) 412
PflMI CCANNNNNTGG 1 cut(s) 199
PfoI TCCNGGA 1 cut(s) 988
PkrI GCNGC 4 cut(s) 620, 833, 1315, 1416
PleI GAGTC 2 cut(s) 13, 1054
PpsI GAGTC 2 cut(s) 13, 1054
PpuMI RGGWCCY 1 cut(s) 1239
PscI ACATGT 1 cut(s) 874
PsiI TTATAA 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 1239
Psp6I CCWGG 1 cut(s) 964
PspGI CCWGG 1 cut(s) 964
PspN4I GGNNCC 1 cut(s) 1270
PspPI GGNCC 1 cut(s) 1239
PspPPI RGGWCCY 1 cut(s) 1239
PspXI VCTCGAGB 1 cut(s) 1069
PstNI CAGNNNCTG 1 cut(s) 1585
PsuI RGATCY 2 cut(s) 508, 1549
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
SaqAI TTAA 5 cut(s) 129, 189, 585, 1188, 1364
SatI GCNGC 4 cut(s) 619, 832, 1314, 1415
Sau3AI GATC 2 cut(s) 508, 1549
Sau96I GGNCC 1 cut(s) 1239
ScaI AGTACT 1 cut(s) 88
SchI GAGTC 2 cut(s) 14, 1054
ScrFI CCNGG 2 cut(s) 966, 990
SduI GDGCHC 3 cut(s) 323, 1081, 1168
SfaNI GCATC 4 cut(s) 61, 544, 843, 1043
SfcI CTRYAG 1 cut(s) 246
Sfr274I CTCGAG 1 cut(s) 1069
SfuI TTCGAA 1 cut(s) 788
SinI GGWCC 1 cut(s) 1239
SlaI CTCGAG 1 cut(s) 1069
SmlI CTYRAG 2 cut(s) 1069, 1363
SmoI CTYRAG 2 cut(s) 1069, 1363
SphI GCATGC 1 cut(s) 986
SsiI CCGC 6 cut(s) 601, 734, 955, 1041, 1395, 1414
StyD4I CCNGG 2 cut(s) 964, 988
StyI CCWWGG 4 cut(s) 193, 493, 970, 1215
TaaI ACNGT 2 cut(s) 407, 1496
TaqI TCGA 7 cut(s) 308, 317, 416, 434, 723, 788, 1070
TaqII GACCGA 1 cut(s) 738
TatI WGTACW 1 cut(s) 86
TauI GCSGC 1 cut(s) 1417
Tru1I TTAA 5 cut(s) 129, 189, 585, 1188, 1364
Tru9I TTAA 5 cut(s) 129, 189, 585, 1188, 1364
TscAI CASTG 1 cut(s) 620
TseFI GTSAC 2 cut(s) 325, 1367
TseI GCWGC 3 cut(s) 618, 831, 1313
Tsp45I GTSAC 2 cut(s) 325, 1367
TspDTI ATGAA 9 cut(s) 154, 270, 339, 453, 617, 867, 1026, 1206, 1271
TspRI CASTG 1 cut(s) 620
Van91I CCANNNNNTGG 1 cut(s) 199
Vha464I CTTAAG 1 cut(s) 1363
VpaK11BI GGWCC 1 cut(s) 1239
XapI RAATTY 5 cut(s) 641, 993, 1293, 1487, 1561
XbaI TCTAGA 1 cut(s) 422
XceI RCATGY 3 cut(s) 878, 986, 1352
XcmI CCANNNNNNNNNTGG 2 cut(s) 1175, 1582
XhoI CTCGAG 1 cut(s) 1069
XmiI GTMKAC 2 cut(s) 670, 896
ZrmI AGTACT 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.