FvH4_4g29700

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014727
Physical Location & Seq
Reverse (-)
29733821 .. 29737285
3465 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29700.t1

Sequence Viewer

Length: 3465 bp
ATGGAATCCCCAATTCCCGTCGAAAGCCCTAAAACCTCCGGAGATCCGGAACCCTCCGATTCCGCATTGCAAGTCGTCGACGGCGGCAGCGACGTCGCCGGAGCCGACTCGATCCTCGACGTCTCCGGCCAGAGCCTAGACTTTCCGATCGGTGAGAACGCCGGCGACGACGTCGGCGATCTATACCTCTACAAAAATGTGTACAATCTACTGCCGAAATCCATCGCCGGTATAAAAGGCTTGAAGACGCTAAAGTTCTTCGGCAACGAGATCAATCTGTTCTCGTCACCGGAGATCGGCGACTTGGTGAGGCTAGAATGCCTGAGGATCTCGTCGCCGGAGTTCGACGGCTTGCCGCTGCACAAGTTCAAGGGGCTGAAGGAGCTGGAGCTCTCCAAGGTCACGTCTCGGTGCTCGGCGGTTCCGATTCTGAGCCAGGTCGCTGGGCTGAAGCTCCTGACGAAGCTATCGGTATGTTATTTCTCTATAACTTTTCTACCGCCGGAGATTTGCTGCTTGAAGAGCTTGGAGTATCTGGACCTTTCGTTCAACAAGATGAAGACTTTGCCTGCAGAAATTGGGAATTTGAATGCTCTGGTTTCGTTGAGAGTTGCGAATAATAAACTGGAGGCATTGCCTGAGGAGTTGTCCAGATTGGCTGTGCTGGAGAGCCTGGACTTGTCGCATAATAGGCTGACGTCACTTGGGGATGTTGATTTTGGGTTGATGATTAGCCTCAAGAGTTTGAATCTCAAGTACAATAGGCTGAAGAGTGAGTGTGTAGTGGCGCCTTCGTCAATATGCTGTGATTTGGATGGGAATGGAAATGATGTGTGTGGCGATGAGTCTGGCAGTTCTTCAGTTGAGTTGGATTGCTCGTCGAATGTGAGTAGTGTGTCTAATAAATCGAAAAGTAGGTTTCTTGCAAGTTGGAGGTCGGATGGGAGGAAGTGTTATATTCGGTGGAGAGGTCCAATGCCGATGCGTGTGAAGGAAGTTGGAGAAAGCAAAGCAGGAAATGTAGATGTTACTGATTCTGTAGCAGATGAGGATGAGGCTGAAGGATGTCGAGAAGCTGAAAGTTGCTCATTTCTCAACTCTACTGCAGTAGATGAAGGTGATGAAAAAGATTGTGATGATGAAGTATCCTCAAACAGTCAAGAGGTTGCAGGTGAGCAAGATGAAGCGTTATGTTCAGAAATGTTAAAAGCCACTTCTGGATCCAAGAGGAAAGAGCATGTCGGGGATCTGAAGAAGCCGAATTGCAAGAATTTAAAGTCAGCTGGAATTAGTGGTGAATCTAGTCATGCTGTTTCATTAGAGCAAGGTGATAAAAACAAATACGAGAATGAAGCACCCTCAGCTAGTCAAGAGGTTGTTGGAGAGAAAGATGAAGAAGCATCCCCTTTAGAAAAATCAGATCCTACTTCAGGATCCAAGAGGAAGAATTCAAATGGTGGTGAGAATGTTAAGCAGCCAGATTGCGAGAATGGTGATGGCAATGTTAAGCAGCCAGATGGCGAGAATGGCGATGGGGATGTTAAGCACCCAGATTGCAAGGATGGTGTTGGGGAGAATAAGCAGCGCAAGAGATTACGTCTTGATCAACTACATAGTCATGATTGTTCAAGCATATCTCAAAAATTTAGTGATATGTCTTTTTGCAGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCTGGACGTGATAGGCCGTTTATGCCTCTAGAGAGTTATGATGGAAAGTTCCCTCTTGACTCGCGTGAAGTTATTTTCTTGGACAGCGAAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGCGGTTCGCCATTTGAATGAGTTAAATGACAATTGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTATTTGTATCAGATCATTTTGGAGGGAGTGATAGGGTTGCTGGTATTGAAAGGAGACGAAAAGGTAGTCCTGATGCAAATGTCAGGAAGCCTTTTGTATGCACCTGCTCAACTAGAAACGGTGAACCTTGTAATTTATCCACCAAACCATACTTGGAGACAGTTGAAGATATTTCTTTTGCTGATATCTCTGAGAAATCTCTCCGCTCTATTAAAGCAAGACGTAAATCTATCCTAGTTCCTATAGGAACTGTGCAGTATGGTGTTTGTAGACATAGAGCATTGCTATTGAAGTATCTTTGTGATCGGATGGAGCCTCGAGTACCTTGTGAGCTTGTGAGGGGATACCTGGATTTCATGCCCCATGCTTGGAATGTGATACCAGTCAAGAGAGTGGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCAAATGACATCAGAAAGGAAACAGATCCTGAATATTATTGTAGGTATATGCCTATGAGTCGGATCAAAGTTCCTCTTTCTGGAAGCAACTTTGGTCATTCTTTGCTACATAATTTGCCCTTTATTGATGGAACTGTGAACAGATCTGGCAGTTCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTGGCAGCTAAAATTCGTACTTTGGAGGTAGATGGGACTTCAGGGGATGATGTTAGGAAGTTCGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCAACACCCCTGCATAGTAGAGATGTATGGACATCAGATATCGTCCAAGTGGGCTCCTTCAGTGAATGGCACTCCTGGCTCCCGTACTCTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCATGAAGAGTTACATAGAAAAGCTATCAAAAGCTGGTGAGAAGCATGTCCCTGTTCATATAGCTTTGTGCATTGCTAAAGATGTTGCTTCTGCATTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACATTAAAAGTGAGAACATATTGATTGATTTGGATAGCAAGACAGCCAATGGAGCACCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTAAGGTCATACTTGCATTCTTGCCTTGTGGCTCATGTAGGGATACCTCCTCCTAATGTTTGTGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTAAGGACAATGCATGAGCAAAATTTTTATGGACTTGAAGTTGATATCTGGTCGTTTGGATGCCTTCTTTATGAATTATTGACTCTGCAACTTCCCCATTCGGGAGTAGCTGATTTGGAGATACATGACCTTATTGTGATGGGACAACGACCAAAGTTAACAGAAGAGTTGGAGGCGCTTCTGCTATTTATGGACGAGCCAGCAGCTCGATCAGCTGCAGAGCTTGGGGGAACAGAGGCTGACTTAGACACACTAGCATTTCTTGCTGACTTGTTCTGCAAATGTACAGAAGAGAATCCGCAGAGTCGTCCAACAGCTGACAACGTCTACAAATTGTTGCTCGAACACAGCATGAAGCTCTCTAGTGAAACTAGGTTAAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1155

Amino Acids

127.07

Weight (kDa)

5.13

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 118 - 258 5e-13 Leucine-rich repeat region
LRR_8 PF13855 153 - 209 5.9e-07 Leucine rich repeat
LRR_8 PF13855 199 - 256 2e-07 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 697 - 765 3.8e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 863 - 1136 3.3e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 865 - 1082 2.5e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1160, 2015
AatII GACGTC 4 cut(s) 96, 123, 174, 701
Acc36I ACCTGC 2 cut(s) 1160, 2015
AccB1I GGYRCC 2 cut(s) 787, 2977
AccBSI CCGCTC 2 cut(s) 2109, 2975
AccI GTMKAC 3 cut(s) 78, 2173, 3405
AccII CGCG 1 cut(s) 1765
AccIII TCCGGA 2 cut(s) 38, 46
AciI CCGC 9 cut(s) 63, 84, 356, 419, 500, 1835, 2107, 2975, 3377
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 7 cut(s) 583, 1270, 1447, 1643, 1812, 2535, 3100
AcuI CTGAAG 9 cut(s) 398, 470, 788, 843, 1080, 1271, 1413, 2547, 2670
AcyI GRCGYC 5 cut(s) 93, 120, 171, 698, 788
AfaI GTAC 8 cut(s) 203, 758, 2226, 2542, 2591, 2713, 2741, 3364
AfiI CCNNNNNNNGG 5 cut(s) 296, 1430, 2271, 2417, 3179
AjiI CACGTC 2 cut(s) 405, 1709
AjnI CCWGG 4 cut(s) 435, 672, 2250, 2701
AloI GAACNNNNNNTCC 2 cut(s) 530, 562
Alw21I GWGCWC 3 cut(s) 393, 416, 2944
Alw26I GTCTC 6 cut(s) 127, 411, 1951, 2054, 2303, 2880
AlwNI CAGNNNCTG 2 cut(s) 2366, 3317
Ama87I CYCGRG 1 cut(s) 2220
Aor13HI TCCGGA 2 cut(s) 38, 46
AoxI GGCC 2 cut(s) 127, 1715
ApoI RAATTY 7 cut(s) 583, 1270, 1447, 1643, 1812, 2535, 3100
ArsI GACNNNNNNTTYG 2 cut(s) 1954, 1986
AspLEI GCGC 3 cut(s) 790, 1587, 3256
AspS9I GGNCC 2 cut(s) 538, 971
AvaI CYCGRG 1 cut(s) 2220
AvaII GGWCC 2 cut(s) 538, 971
AxyI CCTNAGG 2 cut(s) 323, 639
BamHI GGATCC 2 cut(s) 1220, 1433
BanI GGYRCC 2 cut(s) 787, 2977
BanII GRGCYC 2 cut(s) 393, 2683
BarI GAAGNNNNNNTAC 2 cut(s) 2573, 2605
BbsI GAAGAC 3 cut(s) 251, 566, 1673
Bbv12I GWGCWC 3 cut(s) 393, 416, 2944
BbvCI CCTCAGC 1 cut(s) 1360
BceAI ACGGC 3 cut(s) 97, 364, 1702
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 4 cut(s) 437, 674, 2252, 2703
BciVI GTATCC 3 cut(s) 1156, 2240, 3018
BclI TGATCA 1 cut(s) 1603
BcoDI GTCTC 6 cut(s) 127, 411, 1951, 2054, 2303, 2880
BfmI CTRYAG 5 cut(s) 570, 1038, 1104, 2145, 3294
BfoI RGCGCY 2 cut(s) 791, 3257
BfuAI ACCTGC 2 cut(s) 1160, 2015
BfuI GTATCC 3 cut(s) 1156, 2240, 3018
BglII AGATCT 1 cut(s) 2480
Bme1390I CCNGG 4 cut(s) 437, 674, 2252, 2703
Bme18I GGWCC 2 cut(s) 538, 971
BmeT110I CYCGRG 1 cut(s) 2220
BmgBI CACGTC 2 cut(s) 405, 1709
BmgT120I GGNCC 2 cut(s) 538, 971
BmrFI CCNGG 4 cut(s) 437, 674, 2252, 2703
BmsI GCATC 7 cut(s) 972, 1409, 1690, 1822, 1966, 2317, 3128
BpiI GAAGAC 3 cut(s) 251, 566, 1673
BpmI CTGGAG 4 cut(s) 407, 647, 686, 3054
Bpu10I CCTNAGC 2 cut(s) 1360, 2599
BpuEI CTTGAG 2 cut(s) 722, 737
BsaHI GRCGYC 5 cut(s) 93, 120, 171, 698, 788
BsaJI CCNNGG 1 cut(s) 396
BsaWI WCCGGW 3 cut(s) 38, 46, 289
BsaXI ACNNNNNCTCC 4 cut(s) 937, 967, 2931, 2961
Bsc4I CCNNNNNNNGG 5 cut(s) 296, 1430, 2271, 2417, 3179
Bse118I RCCGGY 2 cut(s) 161, 227
Bse1I ACTGG 2 cut(s) 630, 2285
Bse21I CCTNAGG 2 cut(s) 323, 639
Bse3DI GCAATG 6 cut(s) 65, 632, 1507, 1896, 2183, 2826
BseAI TCCGGA 2 cut(s) 38, 46
BseBI CCWGG 4 cut(s) 437, 674, 2252, 2703
BseDI CCNNGG 1 cut(s) 396
BseLI CCNNNNNNNGG 5 cut(s) 296, 1430, 2271, 2417, 3179
BseMI GCAATG 6 cut(s) 65, 632, 1507, 1896, 2183, 2826
BseMII CTCAG 6 cut(s) 314, 422, 630, 1374, 2085, 2298
BseNI ACTGG 2 cut(s) 630, 2285
BseRI GAGGAG 2 cut(s) 656, 3021
BseYI CCCAGC 1 cut(s) 443
BsgI GTGCAG 2 cut(s) 344, 2177
Bsh1236I CGCG 1 cut(s) 1765
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 2 cut(s) 129, 1717
BshNI GGYRCC 2 cut(s) 787, 2977
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 3 cut(s) 393, 416, 2944
BsiHKCI CYCGRG 1 cut(s) 2220
BsiSI CCGG 9 cut(s) 39, 47, 99, 126, 162, 228, 290, 338, 503
BslFI GGGAC 3 cut(s) 2572, 2789, 3234
BslI CCNNNNNNNGG 5 cut(s) 296, 1430, 2271, 2417, 3179
BsmAI GTCTC 6 cut(s) 127, 411, 1951, 2054, 2303, 2880
BsmBI CGTCTC 3 cut(s) 127, 411, 1951
BsmFI GGGAC 3 cut(s) 2572, 2789, 3234
BsmI GAATGC 4 cut(s) 323, 595, 2863, 2998
BsnI GGCC 2 cut(s) 129, 1717
BsoBI CYCGRG 1 cut(s) 2220
Bsp1286I GDGCHC 4 cut(s) 393, 416, 2683, 2944
Bsp13I TCCGGA 2 cut(s) 38, 46
Bsp1407I TGTACA 2 cut(s) 201, 3362
BspACI CCGC 9 cut(s) 63, 84, 356, 419, 500, 1835, 2107, 2975, 3377
BspANI GGCC 2 cut(s) 129, 1717
BspCNI CTCAG 6 cut(s) 315, 423, 631, 1373, 2086, 2299
BspEI TCCGGA 2 cut(s) 38, 46
BspFNI CGCG 1 cut(s) 1765
BspHI TCATGA 1 cut(s) 1618
BspMAI CTGCAG 3 cut(s) 574, 1108, 3298
BspMI ACCTGC 2 cut(s) 1160, 2015
BspQI GCTCTTC 1 cut(s) 515
BspT107I GGYRCC 2 cut(s) 787, 2977
BsrBI CCGCTC 2 cut(s) 2109, 2975
BsrDI GCAATG 6 cut(s) 65, 632, 1507, 1896, 2183, 2826
BsrFI RCCGGY 2 cut(s) 161, 227
BsrGI TGTACA 2 cut(s) 201, 3362
BsrI ACTGG 2 cut(s) 630, 2285
BssAI RCCGGY 2 cut(s) 161, 227
BssECI CCNNGG 1 cut(s) 396
BssNI GRCGYC 5 cut(s) 93, 120, 171, 698, 788
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 4 cut(s) 437, 674, 2252, 2703
Bst4CI ACNGT 6 cut(s) 1157, 1679, 2024, 2065, 2155, 2473
Bst6I CTCTTC 5 cut(s) 515, 764, 2756, 3237, 3363
BstACI GRCGYC 5 cut(s) 93, 120, 171, 698, 788
BstAPI GCANNNNNTGC 1 cut(s) 3341
BstAUI TGTACA 2 cut(s) 201, 3362
BstC8I GCNNGC 4 cut(s) 163, 353, 570, 3279
BstENI CCTNNNNNAGG 1 cut(s) 1428
BstFNI CGCG 1 cut(s) 1765
BstH2I RGCGCY 2 cut(s) 791, 3257
BstHHI GCGC 3 cut(s) 790, 1587, 3256
BstMAI GTCTC 6 cut(s) 127, 411, 1951, 2054, 2303, 2880
BstMCI CGRYCG 1 cut(s) 150
BstNI CCWGG 4 cut(s) 437, 674, 2252, 2703
BstNSI RCATGY 3 cut(s) 1241, 2334, 2804
BstSCI CCNGG 4 cut(s) 435, 672, 2250, 2701
BstSFI CTRYAG 5 cut(s) 570, 1038, 1104, 2145, 3294
BstUI CGCG 1 cut(s) 1765
BstV2I GAAGAC 3 cut(s) 251, 566, 1673
BstX2I RGATCY 8 cut(s) 43, 327, 1220, 1246, 1420, 1433, 2362, 2480
BstXI CCANNNNNNTGG 1 cut(s) 443
BstYI RGATCY 8 cut(s) 43, 327, 1220, 1246, 1420, 1433, 2362, 2480
Bsu36I CCTNAGG 2 cut(s) 323, 639
BsuI GTATCC 3 cut(s) 1156, 2240, 3018
BsuRI GGCC 2 cut(s) 129, 1717
BtgZI GCGATG 3 cut(s) 208, 855, 1545
BtrI CACGTC 2 cut(s) 405, 1709
BtsIMutI CAGTG 3 cut(s) 1869, 2507, 2694
BveI ACCTGC 2 cut(s) 1160, 2015
Cac8I GCNNGC 4 cut(s) 163, 353, 570, 3279
CaiI CAGNNNCTG 2 cut(s) 2366, 3317
CciI TCATGA 1 cut(s) 1618
CfoI GCGC 3 cut(s) 790, 1587, 3256
Cfr10I RCCGGY 2 cut(s) 161, 227
Cfr13I GGNCC 2 cut(s) 538, 971
CseI GACGC 1 cut(s) 256
Csp6I GTAC 8 cut(s) 202, 757, 2225, 2541, 2590, 2712, 2740, 3363
CviQI GTAC 8 cut(s) 202, 757, 2225, 2541, 2590, 2712, 2740, 3363
DinI GGCGCC 1 cut(s) 789
DraI TTTAAA 1 cut(s) 1275
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 5 cut(s) 515, 764, 2756, 3237, 3363
EarI CTCTTC 5 cut(s) 515, 764, 2756, 3237, 3363
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 396
Eco24I GRGCYC 2 cut(s) 393, 2683
Eco32I GATATC 3 cut(s) 2089, 2667, 3124
Eco47I GGWCC 2 cut(s) 538, 971
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 9 cut(s) 398, 470, 788, 843, 1080, 1271, 1413, 2547, 2670
Eco81I CCTNAGG 2 cut(s) 323, 639
Eco88I CYCGRG 1 cut(s) 2220
EcoICRI GAGCTC 1 cut(s) 391
EcoNI CCTNNNNNAGG 1 cut(s) 1428
EcoRI GAATTC 1 cut(s) 1447
EcoRII CCWGG 4 cut(s) 435, 672, 2250, 2701
EcoRV GATATC 3 cut(s) 2089, 2667, 3124
EcoT14I CCWWGG 1 cut(s) 396
EcoT22I ATGCAT 2 cut(s) 2332, 3093
EcoT38I GRGCYC 2 cut(s) 393, 2683
EgeI GGCGCC 1 cut(s) 789
EheI GGCGCC 1 cut(s) 789
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 3 cut(s) 127, 411, 1951
FalI AAGNNNNNCTT 2 cut(s) 2397, 2429
FaqI GGGAC 3 cut(s) 2572, 2789, 3234
FbaI TGATCA 1 cut(s) 1603
FblI GTMKAC 3 cut(s) 78, 2173, 3405
FriOI GRGCYC 2 cut(s) 393, 2683
GlaI GCGC 3 cut(s) 789, 1586, 3255
GsaI CCCAGC 1 cut(s) 447
GsuI CTGGAG 4 cut(s) 407, 647, 686, 3054
HaeII RGCGCY 2 cut(s) 791, 3257
HaeIII GGCC 2 cut(s) 129, 1717
HapII CCGG 9 cut(s) 39, 47, 99, 126, 162, 228, 290, 338, 503
HgaI GACGC 1 cut(s) 256
HhaI GCGC 3 cut(s) 790, 1587, 3256
Hin1I GRCGYC 5 cut(s) 93, 120, 171, 698, 788
Hin6I GCGC 3 cut(s) 788, 1585, 3254
HinP1I GCGC 3 cut(s) 788, 1585, 3254
HincII GTYRAC 2 cut(s) 79, 3237
HindII GTYRAC 2 cut(s) 79, 3237
HpaI GTTAAC 1 cut(s) 3237
HpaII CCGG 9 cut(s) 39, 47, 99, 126, 162, 228, 290, 338, 503
Hpy166II GTNNAC 8 cut(s) 79, 202, 2027, 2174, 2299, 2476, 3237, 3406
Hpy8I GTNNAC 8 cut(s) 79, 202, 2027, 2174, 2299, 2476, 3237, 3406
HpyAV CCTTC 7 cut(s) 373, 801, 985, 1055, 1109, 2694, 3152
HpyCH4III ACNGT 6 cut(s) 1157, 1679, 2024, 2065, 2155, 2473
HpyCH4IV ACGT 9 cut(s) 93, 120, 171, 404, 698, 1597, 1708, 2125, 3402
HpySE526I ACGT 9 cut(s) 93, 120, 171, 404, 698, 1597, 1708, 2125, 3402
Hsp92I GRCGYC 5 cut(s) 93, 120, 171, 698, 788
HspAI GCGC 3 cut(s) 788, 1585, 3254
KasI GGCGCC 1 cut(s) 787
Kpn2I TCCGGA 2 cut(s) 38, 46
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 1603
KspAI GTTAAC 1 cut(s) 3237
LguI GCTCTTC 1 cut(s) 515
LweI GCATC 7 cut(s) 972, 1409, 1690, 1822, 1966, 2317, 3128
MaeII ACGT 9 cut(s) 93, 120, 171, 404, 698, 1597, 1708, 2125, 3402
MaeIII GTNAC 5 cut(s) 285, 400, 699, 1027, 2765
MbiI CCGCTC 2 cut(s) 2109, 2975
MfeI CAATTG 1 cut(s) 1864
MflI RGATCY 8 cut(s) 43, 327, 1220, 1246, 1420, 1433, 2362, 2480
MhlI GDGCHC 4 cut(s) 393, 416, 2683, 2944
Mly113I GGCGCC 1 cut(s) 788
MlyI GAGTC 8 cut(s) 101, 854, 1754, 2294, 2305, 2404, 3154, 3391
Mph1103I ATGCAT 2 cut(s) 2332, 3093
MreI CGCCGGCG 1 cut(s) 161
MroI TCCGGA 2 cut(s) 38, 46
MroNI GCCGGC 1 cut(s) 161
MslI CAYNNNNRTG 2 cut(s) 1617, 1848
MspA1I CMGCKG 4 cut(s) 358, 1283, 3293, 3395
MspI CCGG 9 cut(s) 39, 47, 99, 126, 162, 228, 290, 338, 503
MspR9I CCNGG 4 cut(s) 437, 674, 2252, 2703
MunI CAATTG 1 cut(s) 1864
Mva1269I GAATGC 4 cut(s) 323, 595, 2863, 2998
MvaI CCWGG 4 cut(s) 437, 674, 2252, 2703
MvnI CGCG 1 cut(s) 1765
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 1 cut(s) 788
NgoMIV GCCGGC 1 cut(s) 161
NmeAIII GCCGAG 1 cut(s) 395
NmuCI GTSAC 3 cut(s) 285, 400, 699
NsiI ATGCAT 2 cut(s) 2332, 3093
NspI RCATGY 3 cut(s) 1241, 2334, 2804
PaeR7I CTCGAG 1 cut(s) 2220
PagI TCATGA 1 cut(s) 1618
PaqCI CACCTGC 2 cut(s) 1160, 2015
PciSI GCTCTTC 1 cut(s) 515
PcsI WCGNNNNNNNCGW 5 cut(s) 87, 102, 165, 174, 422
PctI GAATGC 4 cut(s) 323, 595, 2863, 2998
PdiI GCCGGC 1 cut(s) 163
PfeI GAWTC 7 cut(s) 5, 59, 427, 748, 1034, 1298, 3373
PflFI GACNNNGTC 2 cut(s) 170, 3401
Ple19I CGATCG 1 cut(s) 150
PleI GAGTC 8 cut(s) 101, 853, 1754, 2294, 2305, 2403, 3154, 3390
PluTI GGCGCC 1 cut(s) 791
PpsI GAGTC 8 cut(s) 101, 853, 1754, 2294, 2305, 2403, 3154, 3390
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 4 cut(s) 435, 672, 2250, 2701
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 4 cut(s) 435, 672, 2250, 2701
PspPI GGNCC 2 cut(s) 538, 971
PspXI VCTCGAGB 1 cut(s) 2220
PstI CTGCAG 3 cut(s) 574, 1108, 3298
PstNI CAGNNNCTG 2 cut(s) 2366, 3317
PsuI RGATCY 8 cut(s) 43, 327, 1220, 1246, 1420, 1433, 2362, 2480
PsyI GACNNNGTC 2 cut(s) 170, 3401
PvuI CGATCG 1 cut(s) 150
PvuII CAGCTG 3 cut(s) 1283, 3293, 3395
RsaI GTAC 8 cut(s) 203, 758, 2226, 2542, 2591, 2713, 2741, 3364
RsaNI GTAC 8 cut(s) 202, 757, 2225, 2541, 2590, 2712, 2740, 3363
RseI CAYNNNNRTG 2 cut(s) 1617, 1848
SacI GAGCTC 1 cut(s) 393
SalI GTCGAC 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 515
Sau96I GGNCC 2 cut(s) 538, 971
SchI GAGTC 8 cut(s) 101, 854, 1754, 2294, 2305, 2404, 3154, 3391
ScrFI CCNGG 4 cut(s) 437, 674, 2252, 2703
SduI GDGCHC 4 cut(s) 393, 416, 2683, 2944
SfaNI GCATC 7 cut(s) 972, 1409, 1690, 1822, 1966, 2317, 3128
SfcI CTRYAG 5 cut(s) 570, 1038, 1104, 2145, 3294
SfoI GGCGCC 1 cut(s) 789
Sfr274I CTCGAG 1 cut(s) 2220
SgrAI CRCCGGYG 1 cut(s) 161
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 2 cut(s) 538, 971
SlaI CTCGAG 1 cut(s) 2220
SmiMI CAYNNNNRTG 2 cut(s) 1617, 1848
SmlI CTYRAG 3 cut(s) 737, 752, 2220
SmoI CTYRAG 3 cut(s) 737, 752, 2220
SsiI CCGC 9 cut(s) 63, 84, 356, 419, 500, 1835, 2107, 2975, 3377
SspDI GGCGCC 1 cut(s) 787
SspI AATATT 1 cut(s) 2372
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 4 cut(s) 435, 672, 2250, 2701
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 6 cut(s) 1157, 1679, 2024, 2065, 2155, 2473
TaiI ACGT 9 cut(s) 96, 123, 174, 407, 701, 1600, 1711, 2128, 3405
TatI WGTACW 5 cut(s) 201, 756, 2589, 2739, 3362
TauI GCSGC 2 cut(s) 87, 358
TfiI GAWTC 7 cut(s) 5, 59, 427, 748, 1034, 1298, 3373
TscAI CASTG 3 cut(s) 1876, 2507, 2694
TseFI GTSAC 3 cut(s) 285, 400, 699
Tsp45I GTSAC 3 cut(s) 285, 400, 699
TspRI CASTG 3 cut(s) 1876, 2507, 2694
Tth111I GACNNNGTC 2 cut(s) 170, 3401
VpaK11BI GGWCC 2 cut(s) 538, 971
XagI CCTNNNNNAGG 1 cut(s) 1428
XapI RAATTY 7 cut(s) 583, 1270, 1447, 1643, 1812, 2535, 3100
XbaI TCTAGA 1 cut(s) 1729
XceI RCATGY 3 cut(s) 1241, 2334, 2804
XcmI CCANNNNNNNNNTGG 1 cut(s) 2053
XhoI CTCGAG 1 cut(s) 2220
XmiI GTMKAC 3 cut(s) 78, 2173, 3405
ZraI GACGTC 4 cut(s) 94, 121, 172, 699
Zsp2I ATGCAT 2 cut(s) 2332, 3093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.