RLG00000006400

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014727
Physical Location & Seq
Forward (+)
6756634 .. 6760584
3951 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006400

Sequence Viewer

Length: 3516 bp
ATGGAAGCCCCAATTTCCGCCGAAAACCCTAAAACCTCCGAAGACCCAGAAACCTCCGATTCCACATTGCAAGTCGTCGACGACGGTGCCGTCGTCCTTGCCGGAGCCGGAGCCGACTCGATCCTCGACGTCTCCGGCCAAAGCCTGGACTTCCCGATTGGCGAGAACTCCGGCGACGACGTCGGGGATCTATACCTCTACAAAAATGTGTACAATTTACTGCCTAAATCCATCGCCGGACTAAAAGGCTTGAAGACGCTTAAGTTCTTCAGCAACGAGATCAATCTGTTCTCATCGCCGGAGATCGGCGACATGGCAAGGCTAGAATGCATGCGGATCTCGTCGCCGGAGTTCGACGGTTTGCCGCTGCACAAATTCAAAGCGCTGAAGGAGCTTGAGCTCTCCAAGGTCACATCTCGGTGCTCGGCGGTTCCCATTTTGAGCCAGATCGCCGGACTCAAGCTCCTCACGAAGCTATCGGTATGTTATTTCTCTATAACTTTTCTTCCTCCGGAGATTTGCTGCTTAGAGAGTTTGGAGTATCTGGATCTTTCGTTCAACAAGATGAAGACTTTGCCGGGAGAAATTGGCAATTTGAATGCTCTGGTGTCGTTGAGAGTTGCCAATAATAAATTGGAGGAATTGCCTGAGGGTTTGTCCAGATTAGCTAGGTTGGAGAACTTGGACTTGTCGCATAATAGGCTGACGTCATTGGGGGGTGTTGATTTTGGGTTGATGCTTAGCCTCCAGAATTTGAATCTCAAGTACAATAGGCTTTCGAGTGAGTGTGTAGTGCCTTCGTGGATATGCTGCGATTTGGATGGGAATGGAAATGATGTATCTGGTGATGAGGCCGGCAGTTCTTCAGTCGAGTTGGTTTGCTCATCGAATGTAGTTAGTGTGTCTAATAAATCAAGAAGTAGGTTTCTAGCAAGTTGGAGGTCGGATGGGAGGAAGTGTTATTTTCGGTGGGGAGCTCCAGGATGCGTAAATAAAGGCAGGATGTGTGAGGTTCTGGATTGTCCCATACCGATGCGTGTGAAGGAAGATGGAGAAAGCAAAACAGAAAATGTAGATGTTACTGATCCTGCAGTAGATGAGGATGAGGATGAGGATGAAGGTTTAGTATCTCGAGAAGCTGAAATTTGTTCGTTTGTTAACTCTACTGCAGTAGATGAAGGTGATAAAAAAGATTGTGAGGTTGAAGTATCCTCAAACAGTCAAGAGGTTGCTGGTGAGCAAGATGAAGCGTTATGGTCAGAAATGTTAAAAGCCACTTCCGGATCCAAGAGGAAGGAGCATGTTGGGGATCTCAGGAAGTCGAATTGCAAGAATCCAAAATCTGGCGGGATTAGTGGTGTATCTGGTTATGATATTTCACTAGATCAAGGTGATAAAAACGATTACGAGAATGAAGTATCCTCAGCCAGTCAAGAGGTTGTCAGTGAGAAAGATGAAGAAGCATCATGTTTAGAAAAATCAGAAACTACTTTGGGATCCAAGAGGAAGAATTCGAACGGTGATGAGCATGTTAAGCAGCCAGATTGTGAGAATGGTGATGGGAATGTTAAGCAGCCAGACTGCAAGAATGGCAATGGGGATGTTAAGCAGCCAGATTGCAAGAATGTTGTTGGGGATAACAAGCAGCACAAGAGATTACGTCTTGATCAAGTACATAGTCATGATTGTTCAAATTTATCTAAAAAATTTAGTGATACATCTTTTTGCAGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCGGGACGTGATAGGCCGTTCATGCCACTGGAGAGTTATGATGAAAATTTCCCTCATGACTCTCGTGAAGTTATTTTCTTGGACAGCGGAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGTGGTTCGCCGTTTGAATGAGTTAAATGATAATCGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTCTTTGTATCAGATCATTTTGGAGGGAGTGATAGGGTTGCTGGTGTTGAACGGAGACACAAAGGTGGTCCTGACGCAAATGTCAGGAAGCCTTTTGTATGCACCTGCTCAACTAGAAACGGTGAACCTATTAATTTATCCACCAAACCGTACTTGCAGACGGTTGAAGATAATGCTTTTGTTGATATCTCTGAGAAATCTCTCCGCTCTATTAAAGCAAGACATGAATCTATCCTAGTTCCTATTGGAACTGTGCAGTATGGTGTTTGCAGACATAGAGCATTGCTATTGAAGTATCTATGTGATCGGATGGAGCCTCGAGTACCCTGTGAACTAGTTAGGGGATACCTGGATTTCATGCCCCATGCTTGGAACATTATACCAGTCAAGAGAGTTGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCGAATGACATGAGAAAGGAAACAGATCCTGAATTTTATTGTAGGTATATTCCTATGAGTCGGATCAAAGTTCCTCTTTCTGGAAGCAACTTAGGTCATTCTTTGCTACATTCTTTGTCCTCTTTTGATGGAACTGTGAACAAATCTGGCAGTCCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTAGCAGCAAAAATGCGTACTTTGGAGGTAGATGGGACTTCAGGGGATGATGTTAGGAAGTTCGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCAACACCCCTGCATAGTAGAGATGTATGGACATCAGATATCATCCAAGTGGGCTCCTTCAGTGAATGGCACTCCTGGCTCCCGTACTCTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCTTAAAGAGTTACATAGAAAAGCTATCAAAAGCTGGTGAGAAGCATGTCCCTGTGCATTTAGCTTTTTGCATTGCTAAAAATGTTGCTTCTGCACTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACATCAAAAGTGAGAACATTTTGATTGATTTGAATAGCAAGACAGCTGATGGGACCCCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTTCAATCGTACTTGCATTCCTGCCTTGTTGCTCATGTAGGAATACCTCCTTCTAATGTTTGCGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGAGAACAATGCACGAGCAAAATATTTATGGACTGGCAGTAGACATCTGGTCGTTTGGATGCCTTCTTTCTGAATTGATGACTTTGCAACTTCCTTACTCGGGAGTAGCTGATTTGGAGATACATGACCTTATTGTGACGGGACAACGACCTAAGTTAACAGATGAGTTGGAGGCGCTTCTGCTATTTATGGACGAGCCAGTGGCTCGATCAGCTGGAGAGCTTGGGGGAACAGAGGCTGACTTAGACACACTAACGTTTCTTGCTGACTTGTTCTGCAAATGTACTGAGGAGAATCCGCAGAGTCGTCCAACAGCTGAGAACATCTACAAATTGTTGCTTGAACACGGCATGCAGCTTACGAACTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1172

Amino Acids

129.25

Weight (kDa)

5.02

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 121 - 258 2.8e-13 Leucine-rich repeat region
LRR_8 PF13855 156 - 212 3.5e-07 Leucine rich repeat
LRR_8 PF13855 202 - 259 8.1e-07 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 711 - 782 1.9e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 884 - 1157 1.7e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 886 - 1102 2.5e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2078
AasI GACNNNNNNGTC 2 cut(s) 89, 2045
AatII GACGTC 3 cut(s) 132, 183, 710
Acc36I ACCTGC 1 cut(s) 2078
AccB1I GGYRCC 2 cut(s) 86, 3040
AccB7I CCANNNNNTGG 2 cut(s) 145, 1343
AccBSI CCGCTC 2 cut(s) 2172, 3038
AccI GTMKAC 2 cut(s) 78, 3183
AccIII TCCGGA 2 cut(s) 511, 1280
AclI AACGTT 1 cut(s) 3398
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 9 cut(s) 374, 751, 1143, 1510, 1693, 1706, 1810, 1875, 2432
AcuI CTGAAG 5 cut(s) 253, 407, 849, 2610, 2733
AcyI GRCGYC 3 cut(s) 129, 180, 707
AfeI AGCGCT 1 cut(s) 384
AfiI CCNNNNNNNGG 6 cut(s) 145, 305, 1343, 2334, 2480, 3242
AflII CTTAAG 1 cut(s) 260
AhdI GACNNNNNGTC 1 cut(s) 3190
AhlI ACTAGT 1 cut(s) 2299
AjiI CACGTC 1 cut(s) 1772
AjnI CCWGG 4 cut(s) 144, 979, 2313, 2764
AleI CACNNNNGTG 1 cut(s) 2028
AloI GAACNNNNNNTCC 2 cut(s) 539, 571
Alw21I GWGCWC 3 cut(s) 402, 425, 979
Alw26I GTCTC 4 cut(s) 136, 2014, 2366, 2943
AlwNI CAGNNNCTG 2 cut(s) 2429, 3380
Ama87I CYCGRG 3 cut(s) 1131, 2283, 3241
Aor13HI TCCGGA 2 cut(s) 511, 1280
Aor51HI AGCGCT 1 cut(s) 384
AoxI GGCC 3 cut(s) 136, 852, 1778
ApoI RAATTY 9 cut(s) 374, 751, 1143, 1510, 1693, 1706, 1810, 1875, 2432
AseI ATTAAT 1 cut(s) 2097
AspLEI GCGC 2 cut(s) 385, 3319
AspS9I GGNCC 2 cut(s) 2033, 3003
AsuC2I CCSGG 1 cut(s) 579
AsuHPI GGTGA 8 cut(s) 857, 1193, 1247, 1403, 1532, 1568, 2099, 2867
AsuII TTCGAA 1 cut(s) 1514
AvaI CYCGRG 3 cut(s) 1131, 2283, 3241
AvaII GGWCC 2 cut(s) 2033, 3003
AxyI CCTNAGG 1 cut(s) 648
BamHI GGATCC 2 cut(s) 1283, 1496
BanI GGYRCC 2 cut(s) 86, 3040
BanII GRGCYC 3 cut(s) 402, 979, 2746
BarI GAAGNNNNNNTAC 2 cut(s) 2636, 2668
BauI CACGAG 2 cut(s) 1827, 3155
BbsI GAAGAC 4 cut(s) 48, 260, 575, 1736
Bbv12I GWGCWC 3 cut(s) 402, 425, 979
BbvCI CCTCAGC 1 cut(s) 1423
BceAI ACGGC 4 cut(s) 74, 1765, 1890, 3505
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 4 cut(s) 146, 981, 2315, 2766
BciVI GTATCC 3 cut(s) 1219, 1429, 2303
BclI TGATCA 1 cut(s) 1666
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 4 cut(s) 136, 2014, 2366, 2943
BcuI ACTAGT 1 cut(s) 2299
BfaI CTAG 8 cut(s) 323, 669, 929, 1382, 2079, 2201, 2300, 3510
BfmI CTRYAG 2 cut(s) 1089, 1167
BfoI RGCGCY 2 cut(s) 386, 3320
BfrI CTTAAG 1 cut(s) 260
BfuAI ACCTGC 1 cut(s) 2078
BfuI GTATCC 3 cut(s) 1219, 1429, 2303
BlpI GCTNAGC 1 cut(s) 740
Bme1390I CCNGG 5 cut(s) 146, 579, 981, 2315, 2766
Bme18I GGWCC 2 cut(s) 2033, 3003
BmeRI GACNNNNNGTC 1 cut(s) 3190
BmeT110I CYCGRG 3 cut(s) 1131, 2283, 3241
BmgBI CACGTC 1 cut(s) 1772
BmgT120I GGNCC 2 cut(s) 2033, 3003
BmrFI CCNGG 5 cut(s) 146, 579, 981, 2315, 2766
BmsI GCATC 7 cut(s) 726, 974, 1023, 1472, 1753, 2380, 3191
BpiI GAAGAC 4 cut(s) 48, 260, 575, 1736
BpmI CTGGAG 5 cut(s) 731, 963, 1814, 3117, 3378
Bpu10I CCTNAGC 2 cut(s) 1423, 2662
Bpu1102I GCTNAGC 1 cut(s) 740
Bpu14I TTCGAA 1 cut(s) 1514
BpuEI CTTGAG 3 cut(s) 416, 443, 746
BpuMI CCSGG 1 cut(s) 579
BsaHI GRCGYC 3 cut(s) 129, 180, 707
BsaJI CCNNGG 1 cut(s) 405
BsaWI WCCGGW 2 cut(s) 511, 1280
BsaXI ACNNNNNCTCC 4 cut(s) 447, 477, 943, 973
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 305, 1343, 2334, 2480, 3242
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 6 cut(s) 1428, 1797, 2348, 2919, 3180, 3341
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 5 cut(s) 65, 1600, 1959, 2246, 2889
BseAI TCCGGA 2 cut(s) 511, 1280
BseBI CCWGG 4 cut(s) 146, 981, 2315, 2766
BseDI CCNNGG 1 cut(s) 405
BseLI CCNNNNNNNGG 6 cut(s) 145, 305, 1343, 2334, 2480, 3242
BseMI GCAATG 5 cut(s) 65, 1600, 1959, 2246, 2889
BseMII CTCAG 8 cut(s) 639, 1327, 1437, 2148, 2361, 3134, 3420, 3450
BseNI ACTGG 6 cut(s) 1428, 1797, 2348, 2919, 3180, 3341
BseRI GAGGAG 2 cut(s) 455, 3446
BsgI GTGCAG 3 cut(s) 353, 2240, 2895
BshFI GGCC 3 cut(s) 138, 854, 1780
BshNI GGYRCC 2 cut(s) 86, 3040
BsiHKAI GWGCWC 3 cut(s) 402, 425, 979
BsiHKCI CYCGRG 3 cut(s) 1131, 2283, 3241
BslFI GGGAC 6 cut(s) 1008, 1782, 2635, 2852, 3016, 3297
BslI CCNNNNNNNGG 6 cut(s) 145, 305, 1343, 2334, 2480, 3242
BsmAI GTCTC 4 cut(s) 136, 2014, 2366, 2943
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 6 cut(s) 1008, 1782, 2635, 2852, 3016, 3297
BsmI GAATGC 4 cut(s) 332, 604, 2926, 3061
BsnI GGCC 3 cut(s) 138, 854, 1780
BsoBI CYCGRG 3 cut(s) 1131, 2283, 3241
Bsp119I TTCGAA 1 cut(s) 1514
Bsp1286I GDGCHC 4 cut(s) 402, 425, 979, 2746
Bsp13I TCCGGA 2 cut(s) 511, 1280
Bsp1407I TGTACA 1 cut(s) 210
Bsp1720I GCTNAGC 1 cut(s) 740
BspANI GGCC 3 cut(s) 138, 854, 1780
BspCNI CTCAG 8 cut(s) 640, 1326, 1436, 2149, 2362, 3135, 3421, 3451
BspEI TCCGGA 2 cut(s) 511, 1280
BspHI TCATGA 2 cut(s) 1681, 1819
BspMAI CTGCAG 2 cut(s) 1093, 1171
BspMI ACCTGC 1 cut(s) 2078
BspT104I TTCGAA 1 cut(s) 1514
BspT107I GGYRCC 2 cut(s) 86, 3040
BspTI CTTAAG 1 cut(s) 260
BsrBI CCGCTC 2 cut(s) 2172, 3038
BsrDI GCAATG 5 cut(s) 65, 1600, 1959, 2246, 2889
BsrFI RCCGGY 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 6 cut(s) 1428, 1797, 2348, 2919, 3180, 3341
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 1 cut(s) 405
BssNI GRCGYC 3 cut(s) 129, 180, 707
BssSI CACGAG 2 cut(s) 1827, 3155
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 2 cut(s) 1827, 3155
Bst2UI CCWGG 4 cut(s) 146, 981, 2315, 2766
Bst6I CTCTTC 1 cut(s) 3051
BstACI GRCGYC 3 cut(s) 129, 180, 707
BstAFI CTTAAG 1 cut(s) 260
BstAUI TGTACA 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 1514
BstC8I GCNNGC 3 cut(s) 332, 856, 3494
BstH2I RGCGCY 2 cut(s) 386, 3320
BstHHI GCGC 2 cut(s) 385, 3319
BstMAI GTCTC 4 cut(s) 136, 2014, 2366, 2943
BstMWI GCNNNNNNNGC 7 cut(s) 391, 700, 1534, 1590, 1786, 2766, 3353
BstNI CCWGG 4 cut(s) 146, 981, 2315, 2766
BstNSI RCATGY 6 cut(s) 334, 1304, 1532, 2397, 2867, 3496
BstSCI CCNGG 5 cut(s) 144, 577, 979, 2313, 2764
BstSFI CTRYAG 2 cut(s) 1089, 1167
BstV2I GAAGAC 4 cut(s) 48, 260, 575, 1736
BstX2I RGATCY 7 cut(s) 187, 336, 547, 1283, 1309, 1496, 2425
BstXI CCANNNNNNTGG 1 cut(s) 1897
BstYI RGATCY 7 cut(s) 187, 336, 547, 1283, 1309, 1496, 2425
Bsu36I CCTNAGG 1 cut(s) 648
BsuI GTATCC 3 cut(s) 1219, 1429, 2303
BsuRI GGCC 3 cut(s) 138, 854, 1780
BtgZI GCGATG 2 cut(s) 217, 279
BtrI CACGTC 1 cut(s) 1772
BtsIMutI CAGTG 7 cut(s) 1450, 1790, 1932, 2570, 2757, 2912, 3348
BveI ACCTGC 1 cut(s) 2078
Cac8I GCNNGC 3 cut(s) 332, 856, 3494
CaiI CAGNNNCTG 2 cut(s) 2429, 3380
CciI TCATGA 2 cut(s) 1681, 1819
CfoI GCGC 2 cut(s) 385, 3319
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 2 cut(s) 2033, 3003
CseI GACGC 2 cut(s) 265, 2048
DrdI GACNNNNNNGTC 2 cut(s) 89, 2045
DriI GACNNNNNGTC 1 cut(s) 3190
DseDI GACNNNNNNGTC 2 cut(s) 89, 2045
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 3051
Eam1105I GACNNNNNGTC 1 cut(s) 3190
EarI CTCTTC 1 cut(s) 3051
EciI GGCGGA 1 cut(s) 7
Ecl136II GAGCTC 2 cut(s) 400, 977
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 3 cut(s) 402, 979, 2746
Eco32I GATATC 2 cut(s) 2152, 2730
Eco47I GGWCC 2 cut(s) 2033, 3003
Eco47III AGCGCT 1 cut(s) 384
Eco53kI GAGCTC 2 cut(s) 400, 977
Eco57I CTGAAG 5 cut(s) 253, 407, 849, 2610, 2733
Eco81I CCTNAGG 1 cut(s) 648
Eco88I CYCGRG 3 cut(s) 1131, 2283, 3241
EcoICRI GAGCTC 2 cut(s) 400, 977
EcoO109I RGGNCCY 1 cut(s) 3003
EcoRI GAATTC 1 cut(s) 1510
EcoRII CCWGG 4 cut(s) 144, 979, 2313, 2764
EcoRV GATATC 2 cut(s) 2152, 2730
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 2 cut(s) 332, 2395
EcoT38I GRGCYC 3 cut(s) 402, 979, 2746
ErhI CCWWGG 1 cut(s) 405
Esp3I CGTCTC 1 cut(s) 136
FalI AAGNNNNNCTT 2 cut(s) 2460, 2492
FaqI GGGAC 6 cut(s) 1008, 1782, 2635, 2852, 3016, 3297
FauI CCCGC 2 cut(s) 1340, 1759
FbaI TGATCA 1 cut(s) 1666
FblI GTMKAC 2 cut(s) 78, 3183
FriOI GRGCYC 3 cut(s) 402, 979, 2746
FspBI CTAG 8 cut(s) 323, 669, 929, 1382, 2079, 2201, 2300, 3510
GlaI GCGC 2 cut(s) 384, 3318
GsuI CTGGAG 5 cut(s) 731, 963, 1814, 3117, 3378
HaeII RGCGCY 2 cut(s) 386, 3320
HaeIII GGCC 3 cut(s) 138, 854, 1780
HgaI GACGC 2 cut(s) 265, 2048
HhaI GCGC 2 cut(s) 385, 3319
Hin1I GRCGYC 3 cut(s) 129, 180, 707
Hin6I GCGC 2 cut(s) 383, 3317
HinP1I GCGC 2 cut(s) 383, 3317
HincII GTYRAC 4 cut(s) 79, 1159, 2362, 3300
HindII GTYRAC 4 cut(s) 79, 1159, 2362, 3300
HpaI GTTAAC 2 cut(s) 1159, 3300
HphI GGTGA 8 cut(s) 857, 1193, 1247, 1403, 1532, 1568, 2099, 2867
Hpy166II GTNNAC 9 cut(s) 79, 211, 1159, 2090, 2297, 2362, 2539, 3184, 3300
Hpy8I GTNNAC 9 cut(s) 79, 211, 1159, 2090, 2297, 2362, 2539, 3184, 3300
HpyAV CCTTC 9 cut(s) 382, 807, 1036, 1112, 1172, 1288, 2757, 3105, 3215
HpyCH4IV ACGT 6 cut(s) 129, 180, 707, 1660, 1771, 3398
HpyF10VI GCNNNNNNNGC 7 cut(s) 391, 700, 1534, 1590, 1786, 2766, 3353
HpySE526I ACGT 6 cut(s) 129, 180, 707, 1660, 1771, 3398
Hsp92I GRCGYC 3 cut(s) 129, 180, 707
HspAI GCGC 2 cut(s) 383, 3317
KflI GGGWCCC 1 cut(s) 3003
Kpn2I TCCGGA 2 cut(s) 511, 1280
KroI GCCGGC 1 cut(s) 854
KroNI GCCGGC 1 cut(s) 856
Ksp22I TGATCA 1 cut(s) 1666
KspAI GTTAAC 2 cut(s) 1159, 3300
LweI GCATC 7 cut(s) 726, 974, 1023, 1472, 1753, 2380, 3191
MaeI CTAG 8 cut(s) 323, 669, 929, 1382, 2079, 2201, 2300, 3510
MaeII ACGT 6 cut(s) 129, 180, 707, 1660, 1771, 3398
MaeIII GTNAC 4 cut(s) 409, 1078, 2828, 3277
MbiI CCGCTC 2 cut(s) 2172, 3038
MflI RGATCY 7 cut(s) 187, 336, 547, 1283, 1309, 1496, 2425
MhlI GDGCHC 4 cut(s) 402, 425, 979, 2746
MlyI GAGTC 7 cut(s) 110, 450, 1817, 2357, 2368, 2467, 3454
MmeI TCCRAC 9 cut(s) 654, 917, 924, 1848, 2360, 2441, 3091, 3291, 3476
Mph1103I ATGCAT 2 cut(s) 332, 2395
MroI TCCGGA 2 cut(s) 511, 1280
MroNI GCCGGC 1 cut(s) 854
MslI CAYNNNNRTG 5 cut(s) 418, 1031, 1680, 2028, 2737
MspA1I CMGCKG 5 cut(s) 367, 1851, 2996, 3356, 3458
MspCI CTTAAG 1 cut(s) 260
MspR9I CCNGG 5 cut(s) 146, 579, 981, 2315, 2766
Mva1269I GAATGC 4 cut(s) 332, 604, 2926, 3061
MvaI CCWGG 4 cut(s) 146, 981, 2315, 2766
MwoI GCNNNNNNNGC 7 cut(s) 391, 700, 1534, 1590, 1786, 2766, 3353
NaeI GCCGGC 1 cut(s) 856
NciI CCSGG 1 cut(s) 579
NgoMIV GCCGGC 1 cut(s) 854
NmeAIII GCCGAG 1 cut(s) 404
NmuCI GTSAC 2 cut(s) 409, 3277
NsiI ATGCAT 2 cut(s) 332, 2395
NspI RCATGY 6 cut(s) 334, 1304, 1532, 2397, 2867, 3496
NspV TTCGAA 1 cut(s) 1514
OliI CACNNNNGTG 1 cut(s) 2028
PaeI GCATGC 2 cut(s) 334, 3496
PaeR7I CTCGAG 2 cut(s) 1131, 2283
PagI TCATGA 2 cut(s) 1681, 1819
PaqCI CACCTGC 1 cut(s) 2078
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 90
PctI GAATGC 4 cut(s) 332, 604, 2926, 3061
PdiI GCCGGC 1 cut(s) 856
PfeI GAWTC 5 cut(s) 59, 757, 1333, 2192, 3436
PflFI GACNNNGTC 1 cut(s) 179
PflMI CCANNNNNTGG 2 cut(s) 145, 1343
PfoI TCCNGGA 1 cut(s) 979
PleI GAGTC 7 cut(s) 110, 450, 1817, 2357, 2368, 2466, 3453
PpsI GAGTC 7 cut(s) 110, 450, 1817, 2357, 2368, 2466, 3453
PpuMI RGGWCCY 1 cut(s) 3003
PshBI ATTAAT 1 cut(s) 2097
Psp124BI GAGCTC 2 cut(s) 402, 979
Psp1406I AACGTT 1 cut(s) 3398
Psp5II RGGWCCY 1 cut(s) 3003
Psp6I CCWGG 4 cut(s) 144, 979, 2313, 2764
PspGI CCWGG 4 cut(s) 144, 979, 2313, 2764
PspPI GGNCC 2 cut(s) 2033, 3003
PspPPI RGGWCCY 1 cut(s) 3003
PspXI VCTCGAGB 1 cut(s) 2283
PstI CTGCAG 2 cut(s) 1093, 1171
PstNI CAGNNNCTG 2 cut(s) 2429, 3380
PsuI RGATCY 7 cut(s) 187, 336, 547, 1283, 1309, 1496, 2425
PsyI GACNNNGTC 1 cut(s) 179
PvuII CAGCTG 3 cut(s) 2996, 3356, 3458
RseI CAYNNNNRTG 5 cut(s) 418, 1031, 1680, 2028, 2737
SacI GAGCTC 2 cut(s) 402, 979
SalI GTCGAC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 2033, 3003
SchI GAGTC 7 cut(s) 110, 450, 1817, 2357, 2368, 2467, 3454
ScrFI CCNGG 5 cut(s) 146, 579, 981, 2315, 2766
SduI GDGCHC 4 cut(s) 402, 425, 979, 2746
SfaNI GCATC 7 cut(s) 726, 974, 1023, 1472, 1753, 2380, 3191
SfcI CTRYAG 2 cut(s) 1089, 1167
Sfr274I CTCGAG 2 cut(s) 1131, 2283
SfuI TTCGAA 1 cut(s) 1514
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 2 cut(s) 2033, 3003
SlaI CTCGAG 2 cut(s) 1131, 2283
SmiMI CAYNNNNRTG 5 cut(s) 418, 1031, 1680, 2028, 2737
SmlI CTYRAG 6 cut(s) 260, 395, 458, 761, 1131, 2283
SmoI CTYRAG 6 cut(s) 260, 395, 458, 761, 1131, 2283
SpeI ACTAGT 1 cut(s) 2299
SphI GCATGC 2 cut(s) 334, 3496
SspI AATATT 1 cut(s) 3166
SspMI CTAG 8 cut(s) 323, 669, 929, 1382, 2079, 2201, 2300, 3510
SstI GAGCTC 2 cut(s) 402, 979
StyD4I CCNGG 5 cut(s) 144, 577, 979, 2313, 2764
StyI CCWWGG 1 cut(s) 405
TaiI ACGT 6 cut(s) 132, 183, 710, 1663, 1774, 3401
TatI WGTACW 6 cut(s) 210, 765, 1672, 2652, 2802, 3425
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 5 cut(s) 59, 757, 1333, 2192, 3436
TscAI CASTG 7 cut(s) 1450, 1797, 1939, 2570, 2757, 2919, 3348
TseFI GTSAC 2 cut(s) 409, 3277
Tsp45I GTSAC 2 cut(s) 409, 3277
TspGWI ACGGA 1 cut(s) 2032
TspRI CASTG 7 cut(s) 1450, 1797, 1939, 2570, 2757, 2919, 3348
Tth111I GACNNNGTC 1 cut(s) 179
Van91I CCANNNNNTGG 2 cut(s) 145, 1343
Vha464I CTTAAG 1 cut(s) 260
VpaK11BI GGWCC 2 cut(s) 2033, 3003
VspI ATTAAT 1 cut(s) 2097
XapI RAATTY 9 cut(s) 374, 751, 1143, 1510, 1693, 1706, 1810, 1875, 2432
XceI RCATGY 6 cut(s) 334, 1304, 1532, 2397, 2867, 3496
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XhoI CTCGAG 2 cut(s) 1131, 2283
XmiI GTMKAC 2 cut(s) 78, 3183
XspI CTAG 8 cut(s) 323, 669, 929, 1382, 2079, 2201, 2300, 3510
ZraI GACGTC 3 cut(s) 130, 181, 708
Zsp2I ATGCAT 2 cut(s) 332, 2395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.