Rw0G011540

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00507
OG0014727
Physical Location & Seq
Forward (+)
64895 .. 68709
3815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G011540.1

Sequence Viewer

Length: 3489 bp
ATGGAAGCCCCAATTTCCGTCGAAAACCCTAAAACCTCCGAAGACCCAAAAACTTCCGATTCCACATTGCAAGTCGCCGTCGCCGTCGCCGGAGCCGGATCCGGAGCCGACTCGATCCTCGACGTCGCCGGAGCCGGAGCCGGAGCCGACTCGATCCTCGACGTCTCCGGCCAAAGCCTGGACTTCCCGATCGGCGAGAACTCCGGCGACGACGTCGGGGATCTATACCTCTACAAAAATGTGTACAATTTACTGCCTAAATCCATCGCCGGACTAAAAGGCTTGAAGACGCTGAAGTTCTTCAGCAACGAGATCAATCTGTTCTCGTCGCCGGAGATCGGCGACATGGCAAGGCTAGAATGCATGAGGATCTCGTCGCCGGAGTTTGACGGTTTGCCGCTGCACAAATTCAAAGCGCTGAAGGAGCTGGAGCTCTCCAAGGTCACATCTCGGTGCTCGGCGGTTCCCATTTTGAGCCAGATCGCCGGACTCACGCTCCTCACGAAGCTATCGGTATGTTATTTCTCTATAACTTTCCTTCCTCCGGAGATTTGCTGCTTAAAGAGTTTGGAGCATCTGGATCTTTCGTTCAACAAGATGAAGACTTTGCCGGGAGAAATTGGCAATTTGAATGCTCTGGTGTCGTTGAGAGTTGCCAATAATAAGTTGGAGGAATTGCCTGAGGGTCTGTCCAGATTAGCTAGGTTGGAGAACCTGGACTTGTCGCATAATAGGCTGACGTCATTGGGGGTTGTTGATTTTGGGTTGATGCTTAGCCTTCGGAATTTGAATCTCAAGTACAATAGGCTTTCGAGTGAGTGTGTAGTGCCTTCGTGGATATGCTGCGATTTGGATGGGAATGGAAATGATGAGTCTGGCAGTTCTTCCGTCGAGTTGGATTGCTCAGCCAATGTAATTAGTGTGTCTAGTAAATCGAGAAGTAGGAAGTGTTATTTTCGGTGGAGAGCTCCAGGATGCGTAAATAAAGGCAGGATGCCCATGCCGATGCGTGTGAAGGAAGATGGAGAAAGCAAAACAGAAAATGTAGATGTTACTGATTCTGCAGTAGATGAGGATGAGAATAAGGATGAAGGTTTAGTATCTCGAGAAGCTGAAATTTGTTTGTTTGTTAACTCTACTGCAGTAGATGAAGGTGATAAAAAAGATTGTGAGGTTGAAGTATCCTCAAACAGTCAAGAGGTTGCTGGTGAGCAAGATGAAGCATTATGGTCAGAAATGTTAAAAGCCACTTCCGGATCCAAGAGGAAGGAGCATGTTGGGGATCTCAAGAAGCCGAATTGCAAGAATCCAAAATCTGGTGGGATTCGTGGTGTATCTAGTTATGATATTTCACTAGATCAAGGTGATAAAAACGATTACGAGAATGAAGTATCCTCAGCCAGTCAAGAGGTTGTCGGTGAGAAAGATGAAGAAGCATCATGTTTAGAAAAATCAGAAACTACTTTGGGATCCAAGAGGAAGAATTCGAATGGTGATAAGCATGTTAAGCAGCCAGATTGTGAGAATGGTGATGGGAATGTTAAGCAGCCAGACTGCAAGAATGGCAATGGGGATGTTAAGCAGCCAGATTGCAAGAATGGTGTTGGGGATAATAAGCAGCACAAGAGATTACGTCTTGATCAAGTACATAGTCATGATTGTTCAAATTTATCTCAAAAATTTAGTGATACGTCTTTTTGCGGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCGGGACGTGATAGGCCGTTCATGCCACTGGAGAGTTATGATGAAAATTTCCCTCTTGACTCTCGTGAAGTTATTTTCTTGGACAGCGGAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGTGGTTCGCCGTTTGAATGAGTTAAATGATAATCGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTCTTTGTGTCAGATCATTTTGGAGGGAGTGATAGGGTTGCTGGTGTTGAACGGAGACGCAAAGGTGGTCCTGACGCAAATGTCAGGAAGCCTTTTGTATGCACTTGCTCAACTAGAAACGGTGAACCTAGTAATTTATCCACCAAACCATACTTGCAGACGGTTGAAGATAATGCTTTTGTTGATATCTCTGAGAAATCTCTCCGCTCTATTAAAGCAAGACATAAATCTATCCTAGTTCCTATAGGAACTGTGCAGTATGGTGTTTGCAGACATAGAGCATTGCTATTGAAGTATCTATGTGATCGGATGGAGCCTCGAGTACCTTGTGAGCTTGTTAGGGGATACCTGGATTTCATGCCCCATGCTTGGAATATTATACCAGTCAAGAGAGTTGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCGAATGACATGAGAAAGGAAACAGATCCTGAATATTATTGTAGGTATATTCCTATGACACGGATCAAAGTTCCTCTTTCTGGAAGCAACTTTGGTTGTTCTTTGCCACATTCTTTGCCCTCTTTCGATGGAACTCTGAACAAATCTGGCAGTTCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTAGCAGCAAAAATGCGTACTTTGGAGGTAGATGGGACTTCAGGGGATGATGTTAGGAAGTTCGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCACCACCCCTGCATAGTAGAGTTGTATGGACATCAGATATCATCCAAGTGGGCTCCTTCAGTGAATGGCACTCCTGGCTCCCGTACTTTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCTTAAAGAGTTACATAGAAAAGCTATCAAAAGCTGGTGAGAAGCATGTCCCTGTGCATTTAGCTGTTTGCATTGCTAAAAATGTTGCTTCTGCACTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACATCAAAAGTGAGAACATTTTGATTGATTTGAATAGCAAGACAGCTGATGGTACCCCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTTCAGTCATACTTGCATTCCTGCCTTGTCGCTCATGTAGGAATACCTCCTCCTAATGTTTGCGTTGGAACGCCTCGCTGGATGGCTCCAGAGGTTCTGAGAACAATGCATGAGCAAAATATTTATGGACTGGAAGTTGACATCTGGTCATTTGGATGCCTTCTTTCCGAATTGTTGACTTTGCAACTTCCTTACTCCGGAGTAGCTGATTTGGAGATACATGACCTTATTGTGACAGGACAACGACCTAAGTTAACAGATGAGTTGGAGGCGCTTCTGCTATTTACGGACGAGCCAGTGGCTCGATCAGCTGGAGAGCTTGGGGAAACAGAGGCTGACTTAGACACACTAACATTTCTTGCTGACTTGTTCTGCAAATGTACTGAGGAGAATCCGCAGAGTCGTCCAACAGCTGAGAACATCTATAAATTGTTGCTCGAACACAGCATGCAGCTTACAAACTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1162

Amino Acids

128.08

Weight (kDa)

5.23

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 130 - 271 3e-15 Leucine-rich repeat region
LRR_8 PF13855 167 - 223 2.3e-07 Leucine rich repeat
LRR_8 PF13855 213 - 270 5.7e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 702 - 773 1.3e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 875 - 1148 5.7e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 877 - 1093 4.1e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2018
AatII GACGTC 4 cut(s) 126, 165, 216, 743
Acc65I GGTACC 1 cut(s) 2975
AccB1I GGYRCC 2 cut(s) 2975, 3013
AccB7I CCANNNNNTGG 2 cut(s) 178, 1316
AccBSI CCGCTC 2 cut(s) 2145, 3011
AccIII TCCGGA 4 cut(s) 101, 544, 1253, 3215
AciI CCGC 8 cut(s) 398, 461, 1701, 1739, 1824, 2143, 3011, 3413
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 8 cut(s) 407, 784, 1116, 1483, 1666, 1679, 1783, 1848
AcuI CTGAAG 6 cut(s) 286, 314, 440, 2583, 2706, 3005
AcyI GRCGYC 4 cut(s) 123, 162, 213, 740
AfeI AGCGCT 1 cut(s) 417
AfiI CCNNNNNNNGG 7 cut(s) 178, 338, 544, 1316, 2307, 2453, 3215
AhdI GACNNNNNGTC 1 cut(s) 3163
AjiI CACGTC 1 cut(s) 1745
AjnI CCWGG 5 cut(s) 177, 714, 970, 2286, 2737
AloI GAACNNNNNNTCC 2 cut(s) 572, 604
Alw21I GWGCWC 3 cut(s) 435, 458, 970
Alw26I GTCTC 4 cut(s) 169, 1987, 2339, 2916
AlwNI CAGNNNCTG 2 cut(s) 2402, 3353
Ama87I CYCGRG 2 cut(s) 1104, 2256
Aor13HI TCCGGA 4 cut(s) 101, 544, 1253, 3215
Aor51HI AGCGCT 1 cut(s) 417
AoxI GGCC 2 cut(s) 169, 1751
ApoI RAATTY 8 cut(s) 407, 784, 1116, 1483, 1666, 1679, 1783, 1848
Asp700I GAANNNNTTC 1 cut(s) 299
Asp718I GGTACC 1 cut(s) 2975
AspLEI GCGC 2 cut(s) 418, 3292
AspS9I GGNCC 1 cut(s) 2006
AsuC2I CCSGG 1 cut(s) 612
AsuHPI GGTGA 8 cut(s) 1166, 1220, 1376, 1430, 1505, 1541, 2072, 2840
AsuII TTCGAA 1 cut(s) 1487
AvaI CYCGRG 2 cut(s) 1104, 2256
AvaII GGWCC 1 cut(s) 2006
AxyI CCTNAGG 1 cut(s) 681
BaeI ACNNNNGTAYC 2 cut(s) 2967, 3000
BamHI GGATCC 3 cut(s) 98, 1256, 1469
BanI GGYRCC 2 cut(s) 2975, 3013
BanII GRGCYC 3 cut(s) 435, 970, 2719
BarI GAAGNNNNNNTAC 2 cut(s) 2609, 2641
BauI CACGAG 1 cut(s) 1800
BbsI GAAGAC 4 cut(s) 48, 293, 608, 1709
Bbv12I GWGCWC 3 cut(s) 435, 458, 970
BbvCI CCTCAGC 1 cut(s) 1396
BceAI ACGGC 4 cut(s) 62, 68, 1738, 1863
BciT130I CCWGG 5 cut(s) 179, 716, 972, 2288, 2739
BciVI GTATCC 3 cut(s) 1192, 1402, 2276
BclI TGATCA 1 cut(s) 1639
BcnI CCSGG 1 cut(s) 612
BcoDI GTCTC 4 cut(s) 169, 1987, 2339, 2916
BfaI CTAG 9 cut(s) 356, 702, 927, 1338, 1355, 2052, 2067, 2174, 3483
BfmI CTRYAG 3 cut(s) 1062, 1140, 2181
BfoI RGCGCY 2 cut(s) 419, 3293
BfuI GTATCC 3 cut(s) 1192, 1402, 2276
BlpI GCTNAGC 2 cut(s) 773, 904
Bme1390I CCNGG 6 cut(s) 179, 612, 716, 972, 2288, 2739
Bme18I GGWCC 1 cut(s) 2006
BmeRI GACNNNNNGTC 1 cut(s) 3163
BmeT110I CYCGRG 2 cut(s) 1104, 2256
BmgBI CACGTC 1 cut(s) 1745
BmgT120I GGNCC 1 cut(s) 2006
BmrFI CCNGG 6 cut(s) 179, 612, 716, 972, 2288, 2739
BmsI GCATC 9 cut(s) 583, 759, 965, 984, 996, 1445, 1726, 2353, 3164
BpiI GAAGAC 4 cut(s) 48, 293, 608, 1709
BpmI CTGGAG 5 cut(s) 449, 954, 1787, 3090, 3351
Bpu10I CCTNAGC 2 cut(s) 1396, 2635
Bpu1102I GCTNAGC 2 cut(s) 773, 904
Bpu14I TTCGAA 1 cut(s) 1487
BpuEI CTTGAG 2 cut(s) 779, 1271
BpuMI CCSGG 1 cut(s) 612
BsaHI GRCGYC 4 cut(s) 123, 162, 213, 740
BsaJI CCNNGG 1 cut(s) 438
BsaWI WCCGGW 4 cut(s) 101, 544, 1253, 3215
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 7 cut(s) 178, 338, 544, 1316, 2307, 2453, 3215
Bse1I ACTGG 6 cut(s) 1401, 1770, 2321, 2892, 3153, 3314
Bse21I CCTNAGG 1 cut(s) 681
Bse3DI GCAATG 5 cut(s) 65, 1573, 1932, 2219, 2862
BseAI TCCGGA 4 cut(s) 101, 544, 1253, 3215
BseBI CCWGG 5 cut(s) 179, 716, 972, 2288, 2739
BseDI CCNNGG 1 cut(s) 438
BseLI CCNNNNNNNGG 7 cut(s) 178, 338, 544, 1316, 2307, 2453, 3215
BseMI GCAATG 5 cut(s) 65, 1573, 1932, 2219, 2862
BseMII CTCAG 8 cut(s) 672, 918, 1410, 2121, 2334, 3107, 3393, 3423
BseNI ACTGG 6 cut(s) 1401, 1770, 2321, 2892, 3153, 3314
BseRI GAGGAG 3 cut(s) 488, 3057, 3419
BsgI GTGCAG 3 cut(s) 386, 2213, 2868
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 2 cut(s) 171, 1753
BshNI GGYRCC 2 cut(s) 2975, 3013
BsiEI CGRYCG 1 cut(s) 192
BsiHKAI GWGCWC 3 cut(s) 435, 458, 970
BsiHKCI CYCGRG 2 cut(s) 1104, 2256
BslFI GGGAC 3 cut(s) 1755, 2608, 2825
BslI CCNNNNNNNGG 7 cut(s) 178, 338, 544, 1316, 2307, 2453, 3215
BsmAI GTCTC 4 cut(s) 169, 1987, 2339, 2916
BsmBI CGTCTC 2 cut(s) 169, 1987
BsmFI GGGAC 3 cut(s) 1755, 2608, 2825
BsmI GAATGC 4 cut(s) 365, 637, 2899, 3034
BsnI GGCC 2 cut(s) 171, 1753
BsoBI CYCGRG 2 cut(s) 1104, 2256
Bsp119I TTCGAA 1 cut(s) 1487
Bsp1286I GDGCHC 4 cut(s) 435, 458, 970, 2719
Bsp13I TCCGGA 4 cut(s) 101, 544, 1253, 3215
Bsp1407I TGTACA 1 cut(s) 243
Bsp1720I GCTNAGC 2 cut(s) 773, 904
BspACI CCGC 8 cut(s) 398, 461, 1701, 1739, 1824, 2143, 3011, 3413
BspANI GGCC 2 cut(s) 171, 1753
BspCNI CTCAG 8 cut(s) 673, 917, 1409, 2122, 2335, 3108, 3394, 3424
BspEI TCCGGA 4 cut(s) 101, 544, 1253, 3215
BspHI TCATGA 1 cut(s) 1654
BspMAI CTGCAG 2 cut(s) 1066, 1144
BspT104I TTCGAA 1 cut(s) 1487
BspT107I GGYRCC 2 cut(s) 2975, 3013
BsrBI CCGCTC 2 cut(s) 2145, 3011
BsrDI GCAATG 5 cut(s) 65, 1573, 1932, 2219, 2862
BsrGI TGTACA 1 cut(s) 243
BsrI ACTGG 6 cut(s) 1401, 1770, 2321, 2892, 3153, 3314
BssECI CCNNGG 1 cut(s) 438
BssNI GRCGYC 4 cut(s) 123, 162, 213, 740
BssSI CACGAG 1 cut(s) 1800
BssT1I CCWWGG 1 cut(s) 438
Bst2BI CACGAG 1 cut(s) 1800
Bst2UI CCWGG 5 cut(s) 179, 716, 972, 2288, 2739
Bst4CI ACNGT 6 cut(s) 392, 1193, 1715, 2060, 2101, 2191
Bst6I CTCTTC 1 cut(s) 3024
BstACI GRCGYC 4 cut(s) 123, 162, 213, 740
BstAUI TGTACA 1 cut(s) 243
BstBI TTCGAA 1 cut(s) 1487
BstC8I GCNNGC 1 cut(s) 3467
BstH2I RGCGCY 2 cut(s) 419, 3293
BstHHI GCGC 2 cut(s) 418, 3292
BstMAI GTCTC 4 cut(s) 169, 1987, 2339, 2916
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 7 cut(s) 424, 733, 1507, 1563, 1759, 2739, 3326
BstNI CCWGG 5 cut(s) 179, 716, 972, 2288, 2739
BstNSI RCATGY 5 cut(s) 1277, 1505, 2370, 2840, 3469
BstSCI CCNGG 6 cut(s) 177, 610, 714, 970, 2286, 2737
BstSFI CTRYAG 3 cut(s) 1062, 1140, 2181
BstV2I GAAGAC 4 cut(s) 48, 293, 608, 1709
BstX2I RGATCY 8 cut(s) 98, 220, 369, 580, 1256, 1282, 1469, 2398
BstXI CCANNNNNNTGG 1 cut(s) 1870
BstYI RGATCY 8 cut(s) 98, 220, 369, 580, 1256, 1282, 1469, 2398
Bsu36I CCTNAGG 1 cut(s) 681
BsuI GTATCC 3 cut(s) 1192, 1402, 2276
BsuRI GGCC 2 cut(s) 171, 1753
BtgZI GCGATG 1 cut(s) 250
BtrI CACGTC 1 cut(s) 1745
BtsIMutI CAGTG 6 cut(s) 1763, 1905, 2543, 2730, 2885, 3321
Cac8I GCNNGC 1 cut(s) 3467
CaiI CAGNNNCTG 2 cut(s) 2402, 3353
CciI TCATGA 1 cut(s) 1654
CfoI GCGC 2 cut(s) 418, 3292
Cfr13I GGNCC 1 cut(s) 2006
CseI GACGC 3 cut(s) 298, 2004, 2021
CspCI CAANNNNNGTGG 2 cut(s) 2469, 2504
DrdI GACNNNNNNGTC 1 cut(s) 2018
DriI GACNNNNNGTC 1 cut(s) 3163
DseDI GACNNNNNNGTC 1 cut(s) 2018
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 3024
Eam1105I GACNNNNNGTC 1 cut(s) 3163
EarI CTCTTC 1 cut(s) 3024
Ecl136II GAGCTC 2 cut(s) 433, 968
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 3 cut(s) 435, 970, 2719
Eco32I GATATC 2 cut(s) 2125, 2703
Eco47I GGWCC 1 cut(s) 2006
Eco47III AGCGCT 1 cut(s) 417
Eco53kI GAGCTC 2 cut(s) 433, 968
Eco57I CTGAAG 6 cut(s) 286, 314, 440, 2583, 2706, 3005
Eco81I CCTNAGG 1 cut(s) 681
Eco88I CYCGRG 2 cut(s) 1104, 2256
EcoICRI GAGCTC 2 cut(s) 433, 968
EcoRI GAATTC 1 cut(s) 1483
EcoRII CCWGG 5 cut(s) 177, 714, 970, 2286, 2737
EcoRV GATATC 2 cut(s) 2125, 2703
EcoT14I CCWWGG 1 cut(s) 438
EcoT22I ATGCAT 3 cut(s) 365, 2368, 3129
EcoT38I GRGCYC 3 cut(s) 435, 970, 2719
ErhI CCWWGG 1 cut(s) 438
Esp3I CGTCTC 2 cut(s) 169, 1987
FalI AAGNNNNNCTT 2 cut(s) 2433, 2465
FaqI GGGAC 3 cut(s) 1755, 2608, 2825
FauI CCCGC 1 cut(s) 1732
FbaI TGATCA 1 cut(s) 1639
FriOI GRGCYC 3 cut(s) 435, 970, 2719
FspBI CTAG 9 cut(s) 356, 702, 927, 1338, 1355, 2052, 2067, 2174, 3483
GlaI GCGC 2 cut(s) 417, 3291
GsuI CTGGAG 5 cut(s) 449, 954, 1787, 3090, 3351
HaeII RGCGCY 2 cut(s) 419, 3293
HaeIII GGCC 2 cut(s) 171, 1753
HgaI GACGC 3 cut(s) 298, 2004, 2021
HhaI GCGC 2 cut(s) 418, 3292
Hin1I GRCGYC 4 cut(s) 123, 162, 213, 740
Hin6I GCGC 2 cut(s) 416, 3290
HinP1I GCGC 2 cut(s) 416, 3290
HincII GTYRAC 5 cut(s) 1132, 2335, 3157, 3195, 3273
HindII GTYRAC 5 cut(s) 1132, 2335, 3157, 3195, 3273
HpaI GTTAAC 2 cut(s) 1132, 3273
HphI GGTGA 8 cut(s) 1166, 1220, 1376, 1430, 1505, 1541, 2072, 2840
Hpy166II GTNNAC 7 cut(s) 244, 1132, 2063, 2335, 3157, 3195, 3273
Hpy8I GTNNAC 7 cut(s) 244, 1132, 2063, 2335, 3157, 3195, 3273
HpyCH4III ACNGT 6 cut(s) 392, 1193, 1715, 2060, 2101, 2191
HpyCH4IV ACGT 7 cut(s) 123, 162, 213, 740, 1633, 1691, 1744
HpyF10VI GCNNNNNNNGC 7 cut(s) 424, 733, 1507, 1563, 1759, 2739, 3326
HpySE526I ACGT 7 cut(s) 123, 162, 213, 740, 1633, 1691, 1744
Hsp92I GRCGYC 4 cut(s) 123, 162, 213, 740
HspAI GCGC 2 cut(s) 416, 3290
Kpn2I TCCGGA 4 cut(s) 101, 544, 1253, 3215
KpnI GGTACC 1 cut(s) 2979
Ksp22I TGATCA 1 cut(s) 1639
KspAI GTTAAC 2 cut(s) 1132, 3273
LweI GCATC 9 cut(s) 583, 759, 965, 984, 996, 1445, 1726, 2353, 3164
MaeI CTAG 9 cut(s) 356, 702, 927, 1338, 1355, 2052, 2067, 2174, 3483
MaeII ACGT 7 cut(s) 123, 162, 213, 740, 1633, 1691, 1744
MaeIII GTNAC 4 cut(s) 442, 1051, 2801, 3250
MbiI CCGCTC 2 cut(s) 2145, 3011
MflI RGATCY 8 cut(s) 98, 220, 369, 580, 1256, 1282, 1469, 2398
MhlI GDGCHC 4 cut(s) 435, 458, 970, 2719
MlyI GAGTC 8 cut(s) 104, 143, 483, 881, 1790, 2330, 2341, 3427
MmeI TCCRAC 8 cut(s) 648, 687, 876, 1821, 2333, 3064, 3264, 3449
Mph1103I ATGCAT 3 cut(s) 365, 2368, 3129
MroI TCCGGA 4 cut(s) 101, 544, 1253, 3215
MroXI GAANNNNTTC 1 cut(s) 299
MslI CAYNNNNRTG 5 cut(s) 451, 1004, 1653, 2710, 3172
MspA1I CMGCKG 5 cut(s) 400, 1824, 2969, 3329, 3431
MspR9I CCNGG 6 cut(s) 179, 612, 716, 972, 2288, 2739
Mva1269I GAATGC 4 cut(s) 365, 637, 2899, 3034
MvaI CCWGG 5 cut(s) 179, 716, 972, 2288, 2739
MwoI GCNNNNNNNGC 7 cut(s) 424, 733, 1507, 1563, 1759, 2739, 3326
NciI CCSGG 1 cut(s) 612
NmeAIII GCCGAG 1 cut(s) 437
NmuCI GTSAC 2 cut(s) 442, 3250
NsiI ATGCAT 3 cut(s) 365, 2368, 3129
NspI RCATGY 5 cut(s) 1277, 1505, 2370, 2840, 3469
NspV TTCGAA 1 cut(s) 1487
PaeI GCATGC 1 cut(s) 3469
PaeR7I CTCGAG 2 cut(s) 1104, 2256
PagI TCATGA 1 cut(s) 1654
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 500
PctI GAATGC 4 cut(s) 365, 637, 2899, 3034
PdmI GAANNNNTTC 1 cut(s) 299
PfeI GAWTC 6 cut(s) 59, 790, 1058, 1306, 1324, 3409
PflFI GACNNNGTC 1 cut(s) 212
PflMI CCANNNNNTGG 2 cut(s) 178, 1316
PfoI TCCNGGA 1 cut(s) 970
Ple19I CGATCG 1 cut(s) 192
PleI GAGTC 8 cut(s) 104, 143, 483, 880, 1790, 2330, 2341, 3426
PpsI GAGTC 8 cut(s) 104, 143, 483, 880, 1790, 2330, 2341, 3426
Psp124BI GAGCTC 2 cut(s) 435, 970
Psp6I CCWGG 5 cut(s) 177, 714, 970, 2286, 2737
PspGI CCWGG 5 cut(s) 177, 714, 970, 2286, 2737
PspPI GGNCC 1 cut(s) 2006
PspXI VCTCGAGB 1 cut(s) 2256
PstI CTGCAG 2 cut(s) 1066, 1144
PstNI CAGNNNCTG 2 cut(s) 2402, 3353
PsuI RGATCY 8 cut(s) 98, 220, 369, 580, 1256, 1282, 1469, 2398
PsyI GACNNNGTC 1 cut(s) 212
PvuI CGATCG 1 cut(s) 192
PvuII CAGCTG 3 cut(s) 2969, 3329, 3431
RseI CAYNNNNRTG 5 cut(s) 451, 1004, 1653, 2710, 3172
SacI GAGCTC 2 cut(s) 435, 970
Sau96I GGNCC 1 cut(s) 2006
SchI GAGTC 8 cut(s) 104, 143, 483, 881, 1790, 2330, 2341, 3427
ScrFI CCNGG 6 cut(s) 179, 612, 716, 972, 2288, 2739
SduI GDGCHC 4 cut(s) 435, 458, 970, 2719
SfaNI GCATC 9 cut(s) 583, 759, 965, 984, 996, 1445, 1726, 2353, 3164
SfcI CTRYAG 3 cut(s) 1062, 1140, 2181
Sfr274I CTCGAG 2 cut(s) 1104, 2256
SfuI TTCGAA 1 cut(s) 1487
SinI GGWCC 1 cut(s) 2006
SlaI CTCGAG 2 cut(s) 1104, 2256
SmiMI CAYNNNNRTG 5 cut(s) 451, 1004, 1653, 2710, 3172
SmlI CTYRAG 4 cut(s) 794, 1104, 1286, 2256
SmoI CTYRAG 4 cut(s) 794, 1104, 1286, 2256
SphI GCATGC 1 cut(s) 3469
SsiI CCGC 8 cut(s) 398, 461, 1701, 1739, 1824, 2143, 3011, 3413
SspI AATATT 3 cut(s) 2314, 2408, 3139
SspMI CTAG 9 cut(s) 356, 702, 927, 1338, 1355, 2052, 2067, 2174, 3483
SstI GAGCTC 2 cut(s) 435, 970
StyD4I CCNGG 6 cut(s) 177, 610, 714, 970, 2286, 2737
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 6 cut(s) 392, 1193, 1715, 2060, 2101, 2191
TaiI ACGT 7 cut(s) 126, 165, 216, 743, 1636, 1694, 1747
TatI WGTACW 6 cut(s) 243, 798, 1645, 2625, 2775, 3398
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 6 cut(s) 59, 790, 1058, 1306, 1324, 3409
TscAI CASTG 6 cut(s) 1770, 1912, 2543, 2730, 2892, 3321
TseFI GTSAC 2 cut(s) 442, 3250
Tsp45I GTSAC 2 cut(s) 442, 3250
TspGWI ACGGA 5 cut(s) 7, 877, 2005, 2449, 3320
TspRI CASTG 6 cut(s) 1770, 1912, 2543, 2730, 2892, 3321
Tth111I GACNNNGTC 1 cut(s) 212
Van91I CCANNNNNTGG 2 cut(s) 178, 1316
VpaK11BI GGWCC 1 cut(s) 2006
XapI RAATTY 8 cut(s) 407, 784, 1116, 1483, 1666, 1679, 1783, 1848
XceI RCATGY 5 cut(s) 1277, 1505, 2370, 2840, 3469
XcmI CCANNNNNNNNNTGG 1 cut(s) 664
XhoI CTCGAG 2 cut(s) 1104, 2256
XmnI GAANNNNTTC 1 cut(s) 299
XspI CTAG 9 cut(s) 356, 702, 927, 1338, 1355, 2052, 2067, 2174, 3483
ZraI GACGTC 4 cut(s) 124, 163, 214, 741
Zsp2I ATGCAT 3 cut(s) 365, 2368, 3129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.