Rroxscaffold_5G00379100

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014727
Physical Location & Seq
Reverse (-)
59607145 .. 59610635
3491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379100.1

Sequence Viewer

Length: 3396 bp
ATGGAAGCCCCAATTTCTGTCGAAAGCCCTAAAACCTCCGAAGACCCAGAAACCTCCGATTCCACATTGCAAGTCGTCGACGACGGTGCCGTCGCCGGAGCCGGAGCCGACTCGATCATCGACGTCTCCGGCCAAAGCCTGGACTTCCCGATCGGCGAGAACTCCGGCGACGACGTCAGGGATCTATACCTCTACAAAAATGTGTACAATTTACTGCCTAAATCCATCGCCGGACTAAAAGGCTTGAAGACGCTGAAGTTCTTCAGCAACGAGATCAATCTGTTCTCGTCGCCGGAGATCGGCGACATGGCAAGGCTAGAATGCATGAGGATCTCGTCGCCGGAGTTCGACGGTTTGCCGCTGCACAAATTCAAAGCGCTGAAGGAGCTGGAGCTCTCCAAGGTCACATCTCGGTGCTCGGCGGTTCCCATTTTGAGCCAGATCGCCGGACTCAAGCTCCTCACGAAGCTTTCGGTATGTTATTTCTCTATAACTTTCCTTCCTCCGGAGATTTGCTGCTTAAAGAGTTTGGAGCATCTGGATCTTTCGTTCAACAAGATGAAGACTTTGCCGGGAGAAATTGGCAATTTGAGTGCTCTGGCTGACGTCATTGGGGGTGTTGATTTTGGGTTGATGCTTAGCCTCCGGAATTTGAATCTCAAGTACAATAGGCTTTCGAGTGAGTGTGTAGTGCCTTCGTGGATATGCTGCGATTTGGATGGGAATGGAAATGATGTGTCTGGTGATGAGGCCGGCGGTTCTTCGGTGGAGTTGGATTGCTCATCGAATGTAATAAGTGTGTCTGGTAAATCGAGAAGTAGGTTTCTAGCAAGCTGGAGGTCGGATGGGAGGAAGTGTTATTTTCGGTGGAGAGCTCCAGGATGCGTAAATAAAGGCAGGATGCCCATGCCGATGCGTGTGAAGGAAGATGGAGAAAGCAAAACAGAAAATGTAGATGTTACTGAGTCTGCAGTAGATGAGGATGAGGATGAGGATGAAGGTTTAGTATCTCGAGAAGCTGAAATTTGTTTGTTTGTTAACTCTACTGCAGTAGATGAAGGTGATAAAAAAGATTGTGAGGTTGAAGTATCCTCGAACAGTCAAGAGGTTGCTGGTGAGCAAGATGAAGCATTATGGTCAGAAATGTTAAAAGCCACTTCCGGATCCAAGAGGAAGGAGCATGTTGGGGATCTCAAGAAGCCGAATTGCAAGAATCCAAAATCTGGTGGGATTAGTGGTGTATCTAGTGATGATATTTCACTTGATCAAGGTGATAAAAACGATTACGAGAATGAAGTATCCTCAGCCAGTCAAGAGGTTGTCGGTGAGAAAGATGAAGAAGCGTCATGTTTAGAAAAATCAGAAACTACTTTGGGATCCAAGAGGAAGAATTCAAATGGTGATGAGGATGTTAAACAGCCAGATTGCGGGAATGGTGATGGGAATGTTAAGCAGCCAGACTGCAAGAATGGTGATGGGGATGTTAAGCAGCCAGATTGCAAGAATGGTGTTGGGGATAATAAGCAGCACAAGAGATTACGTCTTGATCAAGTACATAGTCATGATTGTTCAAATTTATCTCAAAAATTTAGTGATACGTCTTTTTGCAGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCGGGACGTGATAGGCCGTTCATGCCACTGGAGAGTTATGATGAAAATTTCCCTCTTGACTCGCGTGAAGTTATTTTCTTGGACAGCGGAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGCGGTTCGCCGTTTGAATGAGTTAAATGATAATCGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTCTTTGTATCAGATCATTTTGGAGGGAGTGATAGGGTTGCTGGTGTTGAACGGAGACGCAAAGGTGGTCCTGACGCAAATGTCAGGAAGCCTTTTGTATGCACCTGCTCAACTAGAAACAGTGAACCTATTAATTTATCCACCAAACCATACTTGCAGACGGTTGAAGATAATGCTTTTGTTGATATCTCTGAGAAATCTCTCCGCTCTATTAAAGCAAGACATAAATCTATCCTAGTTCCTATAGGAACTGTGCAGTATGGTGTTTGCAGACATAGAGCATTGCTATTGAAGTATCTATGTGATCGGATGGAGCCTCGAGTACCCTGTGAGCTTGTTAGGGGATACCTGGATTTCATGCCCCATGCTTGGAACATTATACCAGTCAAGAGAGTTGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCAAATGACATGAGAAAGGAAACAGATCCTGAATATTATTGTAGGTATATTCCTATGAGTCGGATCAAAGTTCCTCTTTCTGGAAGCAACTTAGGTCATTCTTTGCTACATTCTTTGTCCTCTTTTGATGGAACTGTGAACAAATCTGGCAGTCCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTAGCAGCAAAAATGCGTACTTTGGAGGTAGATGGGAATTCAGGGGATGATGTTAGGAAGTTCGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCAACACCCCTGCATAGTAGAGATGTATGGACATCAGATATCATCCAAGTGGGCTCCTTCAGTGAATGGCACTCCTGGCTCCCGTACTCTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCTTAAAGAGTTACATAGAAAAGCTATCAAAAGCTGGTGAGAAGCATGTCCCTGTGCATTTAGCTTTTTGCATTGCTAAAAATGTTGCTTCTGCACTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACATCAAAAGTGAGAACATTTTGATTGATTTGAATAGCAAGACAGCTGATGGGACCCCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTTCAGTCGTACTTGCATTCCTGCCTTGTCGCTCATGTAGGAATACCTCCTCCTAATGTTTGTGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGAGAACAATGCATGAGGAAAATATTTATGGACTGGAAGTTGACATCTGGTCGTTTGGATGCCTTCTTTCCGAGTTGTTGACTTTGCAACTTCCTTACTCGGGAGTAGCGGATTTGGAGATACATGACCTTATTGTGACGGGACAACGACCTAAGTTAACAGATGAGTTGGAGGCGCTTCTGCTGTTTATGGACGAGCCAGTGGTTCGATCAGCTGGAGAGCTTGGGGGAACAGAGGCTGACTTGGACGCACTAACATTTCTTGCTGATTTGTTCTGCAAATGTACTGAGGAGAATCCGCAGAGTCGTCCAACAGCTGAGAACATCTACAAATTGTTGCTCGAACACAGCATGCAGCTTACAAACTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1131

Amino Acids

124.79

Weight (kDa)

5.01

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 119 - 203 7.3e-08 Leucine-rich repeat region
EDR1_CTR1_ARMC3_pept PF14381 671 - 742 1.3e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 843 - 1117 1e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 846 - 1062 9.7e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1958
AasI GACNNNNNNGTC 2 cut(s) 89, 1925
AatII GACGTC 3 cut(s) 126, 177, 609
Acc36I ACCTGC 1 cut(s) 1958
AccB1I GGYRCC 2 cut(s) 86, 2920
AccB7I CCANNNNNTGG 2 cut(s) 139, 1223
AccBSI CCGCTC 2 cut(s) 2052, 2918
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 1708
AccIII TCCGGA 3 cut(s) 505, 645, 1160
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 9 cut(s) 368, 649, 1023, 1390, 1573, 1586, 1690, 1755, 2503
AcuI CTGAAG 5 cut(s) 247, 275, 401, 2613, 2912
AcyI GRCGYC 3 cut(s) 123, 174, 606
AfeI AGCGCT 1 cut(s) 378
AfiI CCNNNNNNNGG 8 cut(s) 139, 299, 505, 1223, 1427, 2214, 2360, 3122
AhdI GACNNNNNGTC 1 cut(s) 3070
AjiI CACGTC 1 cut(s) 1652
AjnI CCWGG 4 cut(s) 138, 877, 2193, 2644
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
Alw21I GWGCWC 4 cut(s) 396, 419, 598, 877
Alw26I GTCTC 4 cut(s) 130, 1894, 2246, 2823
AlwNI CAGNNNCTG 2 cut(s) 2309, 3260
Ama87I CYCGRG 3 cut(s) 1011, 2163, 3121
Aor13HI TCCGGA 3 cut(s) 505, 645, 1160
Aor51HI AGCGCT 1 cut(s) 378
AoxI GGCC 3 cut(s) 130, 750, 1658
ApeKI GCWGC 9 cut(s) 361, 516, 708, 1453, 1489, 1525, 2471, 2803, 3376
ApoI RAATTY 9 cut(s) 368, 649, 1023, 1390, 1573, 1586, 1690, 1755, 2503
AseI ATTAAT 1 cut(s) 1977
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 379, 3199
AspS9I GGNCC 2 cut(s) 1913, 2883
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 9 cut(s) 755, 1073, 1127, 1283, 1337, 1412, 1448, 1484, 2747
AvaI CYCGRG 3 cut(s) 1011, 2163, 3121
AvaII GGWCC 2 cut(s) 1913, 2883
BamHI GGATCC 2 cut(s) 1163, 1376
BanI GGYRCC 2 cut(s) 86, 2920
BanII GRGCYC 3 cut(s) 396, 877, 2626
BarI GAAGNNNNNNTAC 2 cut(s) 2516, 2548
BbsI GAAGAC 4 cut(s) 48, 254, 569, 1616
Bbv12I GWGCWC 4 cut(s) 396, 419, 598, 877
BbvCI CCTCAGC 1 cut(s) 1303
BbvI GCAGC 9 cut(s) 348, 503, 695, 1465, 1501, 1537, 2483, 2790, 3388
BceAI ACGGC 3 cut(s) 74, 1645, 1770
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 4 cut(s) 140, 879, 2195, 2646
BciVI GTATCC 3 cut(s) 1099, 1309, 2183
BclI TGATCA 2 cut(s) 1264, 1546
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 4 cut(s) 130, 1894, 2246, 2823
BfaI CTAG 6 cut(s) 317, 827, 1245, 1959, 2081, 3390
BfmI CTRYAG 3 cut(s) 969, 1047, 2088
BfoI RGCGCY 2 cut(s) 380, 3200
BfuAI ACCTGC 1 cut(s) 1958
BfuI GTATCC 3 cut(s) 1099, 1309, 2183
BlpI GCTNAGC 1 cut(s) 638
Bme1390I CCNGG 5 cut(s) 140, 573, 879, 2195, 2646
Bme18I GGWCC 2 cut(s) 1913, 2883
BmeRI GACNNNNNGTC 1 cut(s) 3070
BmeT110I CYCGRG 3 cut(s) 1011, 2163, 3121
BmgBI CACGTC 1 cut(s) 1652
BmgT120I GGNCC 2 cut(s) 1913, 2883
BmrFI CCNGG 5 cut(s) 140, 573, 879, 2195, 2646
BmsI GCATC 9 cut(s) 544, 624, 872, 891, 903, 1633, 1765, 2260, 3071
BpiI GAAGAC 4 cut(s) 48, 254, 569, 1616
BpmI CTGGAG 6 cut(s) 410, 856, 861, 1694, 2997, 3258
Bpu10I CCTNAGC 2 cut(s) 1303, 2542
Bpu1102I GCTNAGC 1 cut(s) 638
BpuEI CTTGAG 3 cut(s) 437, 644, 1178
BpuMI CCSGG 1 cut(s) 573
BsaHI GRCGYC 3 cut(s) 123, 174, 606
BsaJI CCNNGG 1 cut(s) 399
BsaWI WCCGGW 3 cut(s) 505, 645, 1160
BsaXI ACNNNNNCTCC 4 cut(s) 441, 471, 841, 871
Bsc4I CCNNNNNNNGG 8 cut(s) 139, 299, 505, 1223, 1427, 2214, 2360, 3122
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 6 cut(s) 1308, 1677, 2228, 2799, 3060, 3221
Bse3DI GCAATG 4 cut(s) 65, 1839, 2126, 2769
BseAI TCCGGA 3 cut(s) 505, 645, 1160
BseBI CCWGG 4 cut(s) 140, 879, 2195, 2646
BseDI CCNNGG 1 cut(s) 399
BseLI CCNNNNNNNGG 8 cut(s) 139, 299, 505, 1223, 1427, 2214, 2360, 3122
BseMI GCAATG 4 cut(s) 65, 1839, 2126, 2769
BseMII CTCAG 7 cut(s) 954, 1317, 2028, 2241, 3014, 3300, 3330
BseNI ACTGG 6 cut(s) 1308, 1677, 2228, 2799, 3060, 3221
BseRI GAGGAG 3 cut(s) 449, 2964, 3326
BseXI GCAGC 9 cut(s) 348, 503, 695, 1465, 1501, 1537, 2483, 2790, 3388
BsgI GTGCAG 3 cut(s) 347, 2120, 2775
Bsh1236I CGCG 1 cut(s) 1708
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 3 cut(s) 132, 752, 1660
BshNI GGYRCC 2 cut(s) 86, 2920
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 4 cut(s) 396, 419, 598, 877
BsiHKCI CYCGRG 3 cut(s) 1011, 2163, 3121
BslFI GGGAC 4 cut(s) 1662, 2732, 2896, 3177
BslI CCNNNNNNNGG 8 cut(s) 139, 299, 505, 1223, 1427, 2214, 2360, 3122
BsmAI GTCTC 4 cut(s) 130, 1894, 2246, 2823
BsmBI CGTCTC 2 cut(s) 130, 1894
BsmFI GGGAC 4 cut(s) 1662, 2732, 2896, 3177
BsmI GAATGC 3 cut(s) 326, 2806, 2941
BsnI GGCC 3 cut(s) 132, 752, 1660
BsoBI CYCGRG 3 cut(s) 1011, 2163, 3121
Bsp1286I GDGCHC 5 cut(s) 396, 419, 598, 877, 2626
Bsp13I TCCGGA 3 cut(s) 505, 645, 1160
Bsp1407I TGTACA 1 cut(s) 204
Bsp1720I GCTNAGC 1 cut(s) 638
BspANI GGCC 3 cut(s) 132, 752, 1660
BspCNI CTCAG 7 cut(s) 955, 1316, 2029, 2242, 3015, 3301, 3331
BspEI TCCGGA 3 cut(s) 505, 645, 1160
BspFNI CGCG 1 cut(s) 1708
BspHI TCATGA 1 cut(s) 1561
BspMAI CTGCAG 2 cut(s) 973, 1051
BspMI ACCTGC 1 cut(s) 1958
BspT107I GGYRCC 2 cut(s) 86, 2920
BsrBI CCGCTC 2 cut(s) 2052, 2918
BsrDI GCAATG 4 cut(s) 65, 1839, 2126, 2769
BsrFI RCCGGY 1 cut(s) 752
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 6 cut(s) 1308, 1677, 2228, 2799, 3060, 3221
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 1 cut(s) 399
BssNI GRCGYC 3 cut(s) 123, 174, 606
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 4 cut(s) 140, 879, 2195, 2646
Bst4CI ACNGT 8 cut(s) 86, 353, 1100, 1622, 1967, 2008, 2098, 2416
Bst6I CTCTTC 1 cut(s) 2931
BstACI GRCGYC 3 cut(s) 123, 174, 606
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 754, 832, 3374
BstFNI CGCG 1 cut(s) 1708
BstH2I RGCGCY 2 cut(s) 380, 3200
BstHHI GCGC 2 cut(s) 379, 3199
BstMAI GTCTC 4 cut(s) 130, 1894, 2246, 2823
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 4 cut(s) 385, 1666, 1775, 2646
BstNI CCWGG 4 cut(s) 140, 879, 2195, 2646
BstNSI RCATGY 4 cut(s) 1184, 2277, 2747, 3376
BstSCI CCNGG 5 cut(s) 138, 571, 877, 2193, 2644
BstSFI CTRYAG 3 cut(s) 969, 1047, 2088
BstUI CGCG 1 cut(s) 1708
BstV1I GCAGC 9 cut(s) 348, 503, 695, 1465, 1501, 1537, 2483, 2790, 3388
BstV2I GAAGAC 4 cut(s) 48, 254, 569, 1616
BstX2I RGATCY 7 cut(s) 181, 330, 541, 1163, 1189, 1376, 2305
BstYI RGATCY 7 cut(s) 181, 330, 541, 1163, 1189, 1376, 2305
BsuI GTATCC 3 cut(s) 1099, 1309, 2183
BsuRI GGCC 3 cut(s) 132, 752, 1660
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 1652
BtsIMutI CAGTG 7 cut(s) 1670, 1812, 1972, 2450, 2637, 2792, 3228
BveI ACCTGC 1 cut(s) 1958
Cac8I GCNNGC 3 cut(s) 754, 832, 3374
CaiI CAGNNNCTG 2 cut(s) 2309, 3260
CciI TCATGA 1 cut(s) 1561
CfoI GCGC 2 cut(s) 379, 3199
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 2 cut(s) 1913, 2883
CseI GACGC 5 cut(s) 259, 1332, 1911, 1928, 3278
DrdI GACNNNNNNGTC 2 cut(s) 89, 1925
DriI GACNNNNNGTC 1 cut(s) 3070
DseDI GACNNNNNNGTC 2 cut(s) 89, 1925
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 2931
Eam1105I GACNNNNNGTC 1 cut(s) 3070
EarI CTCTTC 1 cut(s) 2931
Ecl136II GAGCTC 2 cut(s) 394, 875
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 3 cut(s) 396, 877, 2626
Eco32I GATATC 2 cut(s) 2032, 2610
Eco47I GGWCC 2 cut(s) 1913, 2883
Eco47III AGCGCT 1 cut(s) 378
Eco53kI GAGCTC 2 cut(s) 394, 875
Eco57I CTGAAG 5 cut(s) 247, 275, 401, 2613, 2912
Eco88I CYCGRG 3 cut(s) 1011, 2163, 3121
EcoICRI GAGCTC 2 cut(s) 394, 875
EcoO109I RGGNCCY 1 cut(s) 2883
EcoRI GAATTC 2 cut(s) 1390, 2503
EcoRII CCWGG 4 cut(s) 138, 877, 2193, 2644
EcoRV GATATC 2 cut(s) 2032, 2610
EcoT14I CCWWGG 1 cut(s) 399
EcoT22I ATGCAT 3 cut(s) 326, 2275, 3036
EcoT38I GRGCYC 3 cut(s) 396, 877, 2626
ErhI CCWWGG 1 cut(s) 399
Esp3I CGTCTC 2 cut(s) 130, 1894
FalI AAGNNNNNCTT 2 cut(s) 2340, 2372
FaqI GGGAC 4 cut(s) 1662, 2732, 2896, 3177
FauI CCCGC 2 cut(s) 1421, 1639
FbaI TGATCA 2 cut(s) 1264, 1546
FblI GTMKAC 1 cut(s) 78
FriOI GRGCYC 3 cut(s) 396, 877, 2626
FspBI CTAG 6 cut(s) 317, 827, 1245, 1959, 2081, 3390
GlaI GCGC 2 cut(s) 378, 3198
GsuI CTGGAG 6 cut(s) 410, 856, 861, 1694, 2997, 3258
HaeII RGCGCY 2 cut(s) 380, 3200
HaeIII GGCC 3 cut(s) 132, 752, 1660
HgaI GACGC 5 cut(s) 259, 1332, 1911, 1928, 3278
HhaI GCGC 2 cut(s) 379, 3199
Hin1I GRCGYC 3 cut(s) 123, 174, 606
Hin6I GCGC 2 cut(s) 377, 3197
HinP1I GCGC 2 cut(s) 377, 3197
HincII GTYRAC 6 cut(s) 79, 1039, 2242, 3064, 3102, 3180
HindII GTYRAC 6 cut(s) 79, 1039, 2242, 3064, 3102, 3180
HindIII AAGCTT 1 cut(s) 467
HpaI GTTAAC 2 cut(s) 1039, 3180
HphI GGTGA 9 cut(s) 755, 1073, 1127, 1283, 1337, 1412, 1448, 1484, 2747
Hpy166II GTNNAC 9 cut(s) 79, 205, 1039, 1970, 2242, 2419, 3064, 3102, 3180
Hpy8I GTNNAC 9 cut(s) 79, 205, 1039, 1970, 2242, 2419, 3064, 3102, 3180
HpyAV CCTTC 9 cut(s) 376, 509, 705, 916, 992, 1052, 1168, 2637, 3095
HpyCH4III ACNGT 8 cut(s) 86, 353, 1100, 1622, 1967, 2008, 2098, 2416
HpyCH4IV ACGT 6 cut(s) 123, 174, 606, 1540, 1598, 1651
HpyF10VI GCNNNNNNNGC 4 cut(s) 385, 1666, 1775, 2646
HpySE526I ACGT 6 cut(s) 123, 174, 606, 1540, 1598, 1651
Hsp92I GRCGYC 3 cut(s) 123, 174, 606
HspAI GCGC 2 cut(s) 377, 3197
KflI GGGWCCC 1 cut(s) 2883
Kpn2I TCCGGA 3 cut(s) 505, 645, 1160
KroI GCCGGC 1 cut(s) 752
KroNI GCCGGC 1 cut(s) 754
Ksp22I TGATCA 2 cut(s) 1264, 1546
KspAI GTTAAC 2 cut(s) 1039, 3180
Lsp1109I GCAGC 9 cut(s) 348, 503, 695, 1465, 1501, 1537, 2483, 2790, 3388
LweI GCATC 9 cut(s) 544, 624, 872, 891, 903, 1633, 1765, 2260, 3071
MaeI CTAG 6 cut(s) 317, 827, 1245, 1959, 2081, 3390
MaeII ACGT 6 cut(s) 123, 174, 606, 1540, 1598, 1651
MaeIII GTNAC 4 cut(s) 403, 958, 2708, 3157
MbiI CCGCTC 2 cut(s) 2052, 2918
MflI RGATCY 7 cut(s) 181, 330, 541, 1163, 1189, 1376, 2305
MhlI GDGCHC 5 cut(s) 396, 419, 598, 877, 2626
MlyI GAGTC 8 cut(s) 104, 444, 974, 1697, 2237, 2248, 2347, 3334
MmeI TCCRAC 8 cut(s) 753, 822, 1728, 2240, 2321, 2971, 3171, 3356
Mph1103I ATGCAT 3 cut(s) 326, 2275, 3036
MroI TCCGGA 3 cut(s) 505, 645, 1160
MroNI GCCGGC 1 cut(s) 752
MroXI GAANNNNTTC 1 cut(s) 260
MslI CAYNNNNRTG 4 cut(s) 412, 911, 1560, 2617
MspA1I CMGCKG 5 cut(s) 361, 1731, 2876, 3236, 3338
MspR9I CCNGG 5 cut(s) 140, 573, 879, 2195, 2646
Mva1269I GAATGC 3 cut(s) 326, 2806, 2941
MvaI CCWGG 4 cut(s) 140, 879, 2195, 2646
MvnI CGCG 1 cut(s) 1708
MwoI GCNNNNNNNGC 4 cut(s) 385, 1666, 1775, 2646
NaeI GCCGGC 1 cut(s) 754
NciI CCSGG 1 cut(s) 573
NgoMIV GCCGGC 1 cut(s) 752
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 2 cut(s) 403, 3157
NsiI ATGCAT 3 cut(s) 326, 2275, 3036
NspI RCATGY 4 cut(s) 1184, 2277, 2747, 3376
PaeI GCATGC 1 cut(s) 3376
PaeR7I CTCGAG 2 cut(s) 1011, 2163
PagI TCATGA 1 cut(s) 1561
PaqCI CACCTGC 1 cut(s) 1958
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 3 cut(s) 326, 2806, 2941
PdiI GCCGGC 1 cut(s) 754
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 4 cut(s) 59, 655, 1213, 3316
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 2 cut(s) 139, 1223
PfoI TCCNGGA 1 cut(s) 877
Ple19I CGATCG 1 cut(s) 153
PleI GAGTC 8 cut(s) 104, 444, 973, 1697, 2237, 2248, 2346, 3333
PpsI GAGTC 8 cut(s) 104, 444, 973, 1697, 2237, 2248, 2346, 3333
PpuMI RGGWCCY 1 cut(s) 2883
PshBI ATTAAT 1 cut(s) 1977
Psp124BI GAGCTC 2 cut(s) 396, 877
Psp5II RGGWCCY 1 cut(s) 2883
Psp6I CCWGG 4 cut(s) 138, 877, 2193, 2644
PspGI CCWGG 4 cut(s) 138, 877, 2193, 2644
PspPI GGNCC 2 cut(s) 1913, 2883
PspPPI RGGWCCY 1 cut(s) 2883
PspXI VCTCGAGB 1 cut(s) 2163
PstI CTGCAG 2 cut(s) 973, 1051
PstNI CAGNNNCTG 2 cut(s) 2309, 3260
PsuI RGATCY 7 cut(s) 181, 330, 541, 1163, 1189, 1376, 2305
PsyI GACNNNGTC 1 cut(s) 173
PvuI CGATCG 1 cut(s) 153
PvuII CAGCTG 3 cut(s) 2876, 3236, 3338
RseI CAYNNNNRTG 4 cut(s) 412, 911, 1560, 2617
SacI GAGCTC 2 cut(s) 396, 877
SalI GTCGAC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 1913, 2883
SchI GAGTC 8 cut(s) 104, 444, 974, 1697, 2237, 2248, 2347, 3334
ScrFI CCNGG 5 cut(s) 140, 573, 879, 2195, 2646
SduI GDGCHC 5 cut(s) 396, 419, 598, 877, 2626
SfaNI GCATC 9 cut(s) 544, 624, 872, 891, 903, 1633, 1765, 2260, 3071
SfcI CTRYAG 3 cut(s) 969, 1047, 2088
Sfr274I CTCGAG 2 cut(s) 1011, 2163
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 2 cut(s) 1913, 2883
SlaI CTCGAG 2 cut(s) 1011, 2163
SmiMI CAYNNNNRTG 4 cut(s) 412, 911, 1560, 2617
SmlI CTYRAG 5 cut(s) 452, 659, 1011, 1193, 2163
SmoI CTYRAG 5 cut(s) 452, 659, 1011, 1193, 2163
SphI GCATGC 1 cut(s) 3376
SspI AATATT 2 cut(s) 2315, 3046
SspMI CTAG 6 cut(s) 317, 827, 1245, 1959, 2081, 3390
SstI GAGCTC 2 cut(s) 396, 877
StyD4I CCNGG 5 cut(s) 138, 571, 877, 2193, 2644
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 8 cut(s) 86, 353, 1100, 1622, 1967, 2008, 2098, 2416
TaiI ACGT 6 cut(s) 126, 177, 609, 1543, 1601, 1654
TatI WGTACW 6 cut(s) 204, 663, 1552, 2532, 2682, 3305
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 4 cut(s) 59, 655, 1213, 3316
TscAI CASTG 7 cut(s) 1677, 1819, 1972, 2450, 2637, 2799, 3228
TseFI GTSAC 2 cut(s) 403, 3157
TseI GCWGC 9 cut(s) 361, 516, 708, 1453, 1489, 1525, 2471, 2803, 3376
Tsp45I GTSAC 2 cut(s) 403, 3157
TspGWI ACGGA 1 cut(s) 1912
TspRI CASTG 7 cut(s) 1677, 1819, 1972, 2450, 2637, 2799, 3228
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 2 cut(s) 139, 1223
VpaK11BI GGWCC 2 cut(s) 1913, 2883
VspI ATTAAT 1 cut(s) 1977
XapI RAATTY 9 cut(s) 368, 649, 1023, 1390, 1573, 1586, 1690, 1755, 2503
XceI RCATGY 4 cut(s) 1184, 2277, 2747, 3376
XhoI CTCGAG 2 cut(s) 1011, 2163
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 6 cut(s) 317, 827, 1245, 1959, 2081, 3390
ZraI GACGTC 3 cut(s) 124, 175, 607
Zsp2I ATGCAT 3 cut(s) 326, 2275, 3036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.