Rh4DG363100

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014727
Physical Location & Seq
Reverse (-)
58447712 .. 58451775
4064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG363100.1

Sequence Viewer

Length: 3480 bp
ATGGAAGCCCCAATTTCCGTCGAAAACCCTAAAACCTCCGAAGACCCAGAAACTTCCGATTCCACATTGCAAGTCGTCGACGACGGCACCGTCGTCGTCGCCGGAGCCGACTCTATCCTCGACGTCTCCGGCCAAAGCCTGGACTTCCCGATCGGCGAGAACTCCGGCGACGACGTCGGGGATCTATACCTCTACAAAAATGTGTATAATTTACTGCCTAAATCCATCACCGGACTAAAAGGCTTGAAGACGCTGAAGTTCTTCAGCAACGAGATCAATCTGTTCTCGTCGCCGGAGATCGGCGACATGGCAAGGCTAGAATGCATGAGGATCTCGTCGCCGGAGTTCGACGGTTTGCCGCTGCACAAATTCAAAGCGCTGAAGGAGCTGGAGCTCTCCAAGGTCACATCTCGGTGCTTGGCGGTTCCCATTTTAAGCCAGATCGCCGGACTCAAGCTCCTCACGAAGCTATCGGTATGTTATTTCTCTATAACTTTCCTTCCTCCGGAGATTTGCTGCTTAAAGAGTTTGGAGCATCTGGATCTTTCGTTCAACAAGATGAAGACCTTGCCGGGAGAAATTGGCAATTTGAATGCTCTGGTGTCGTTGAGAGTTGCCAATAATAAATTGGAGGAATTGCCTGAGGGTTTGTCCAGATTAGCTAGGTTGGAGAACCTGGACTTGTCGCATAATAGGCTGACGTCATTGGGGGTTGTTGATTTTGGGTTGATGCTTAGCCTCCGGAATTTGAATCTCAAGTACAATAGGCTTTCGAGTGAGTGTGTAGTGCCTTCGTGGATATGCTGCGATTTGGATGGGAATGGAAATGATGAGTCTGGCAGTTCTTCAGTCGAGTTGGATTGCTCAGCCAATGTAATTAGTGTGTCTAGTAAATCGAGAAGTAGGTTTCTAGCAAGTTGGAGGTCGGATGGGAGGAAGTGTTATTTTCGGTGGAGAGCTCCAGGATGCGTAAATAAAGGCAGGATGCCCATGCCGATGCGTGTGAAGGAAGATGGAGAAAGCAAAACAGAAAATGTAGATGTTACTGATTCTGCAGTAGATGAGGATGAGGATAAGGATGAAGGTTTAGTATCTCAAGAAGCTGAAATTTGTTTGTTTGTTAACTCTACTACAGTAGATGAAGGTGATAAAAAAGATTGTGAGGTTGAAGTATCCTCAAACAGTCAAGAGGTTGCTGGTGAGCAAGATGAAGCATTATGGTCAGAAATGTTAAAAGCCACTTCCGGATCCAAGAGGAAGGAGCATGTTGGGGATCTCAAGAAGCCGAATTGCAAGAATCCAAAATCTGGTGGGATTAGTGGTGTATCTAGTTATGATATTTCACTAGATCAAGGTGATAAAAACGATTACGAGAATGAAGTATCCTCAGCCAGTCAAGAGGTTGTCGGTGAGAAAGATGAAGAAGCATCATGTTTAGAAAAATCAGAAACTACTTTGGGATCCAAGAGGAAGAATTCGAATGGTGATGAGCATGTTAAGCAGCCAGATTGTGAGAATGGTGATGGGAATGTTAAGCAGCCAGACTGCAAGAATGGCAATGGGGATGTTAAGCAGCCAGATTGCAAGAATGGTGTTGGGGATAATAAGCAGCACAAGAGATTACGTCTTGATCAAGTACATAGTCATGATTGTTCAAATTTATCTCAAAAATTTAGTGATACGTCTTTTTGCGGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCGGGACGTGATAGGCCGTTCATGCCACTGGAGAGTTATGATGAAAATTTCCCTCTTGACTCTCGTGAAGTTATTTTCTTGGACAGCGGAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGTGGTTCGCCGTTTGAATGAGTTAAATGATAATCGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTCTTTGTGTCAGATCATTTTGGAGGGAGTGATAGGGTTGCTGGTGTTGAACGGAGACGCAAAGGTGGTCCTGACGCAAATGTCAGGAAGCCTTTTGTATGCACCTGCTCAACTAGAAACGGTGAACCTATTAATTTATCCACTAAACCATACTTGCAGACGGTTGAAGTTAATGCTTTTGTTGATATCTCTGAGAAATCTCTCTGCTCTATTAAAGCAAGACATAAATCTATCCTAGTTCCTATAGGAACTGTGCAGTATGGTGTTTGCAGACATAGAGCATTGCTATTGAAGTATCTATGTGATCGGATGGAGCCTCGAGTACCTTGTGAGCTTGTTAGGGGATACCTGGATTTCATGCCCCATGCTTGGAATATTATACCAGTCAAGAGAGTTGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCGAATGACATGAGAAAGGAAACAGATCCTGAATATTATTGTAGGTATATTCCTATGACACGGATCAAAGTTCCTCTTTCTGGAAGCAACTTTGGTTGTTCTTTGCCACATTCTTTGCCCTCTTTTGATGGAACTCTGAACAAATCTGGCAGTTCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTAGCAGCAAAAATGCGTACTTTGGAGGTAGATGGGACTTCAGGGGATGATGTTAGGAAGTTCGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCACCACCCCTGCATAGTAGAGTTGTATGGACATCAGATATCATCCAAGTGGGCTCCTTCAGTGAATGGCACTCCTGGCTCCCGTACTTTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCTTAAAGAGTTACATAGAAAAGCTATCAAAAGCTGGGGAGAAGCATGTCCCTCTGCATTTAGCTGTTTGCATTGCTAAAAATGTTGCTTCTGCACTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACATCAAAAGTGAGAACATTTTGATTGATTTGAATAGCAAGACAGCTGATGGGACCCCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTTCAGTCGTACTTGCATTCCTGCCTTGTCGCTCATGTAGGAATACCTCCTCCTAATGTTTGCGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGAGAACAATGCATGAGCAAAATATTTATGGACTGGAAGTTGACATCTGGTCATTTGGATGCCTTCTTTCCGAATTGTTGACTTTGCAACTTCCTTACTCCGGAGTAGCTGATTTGGAGATACATGACCTTATTGTGACAGGACAACGACCTAAGTTAACAGATGAGTTGGAGGCGCTTCTGCTATATATGGACGAGCCAGTGGCTCGATCAGCTGGAGAGCTTGGGGGAACAGAGTCTGACTTAGACACACTAACATTTCTCGTTGACTTGTTCTGCAAATGTACTGAGGAGAATCCGCAGAGTCGTCCAACAGCTGAGAACATCTATAAATTGTTGCTCGAACACAGCATGCAGCTTACAAACTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1159

Amino Acids

128.34

Weight (kDa)

5.14

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 119 - 258 1.9e-14 Leucine-rich repeat region
LRR_8 PF13855 154 - 210 2.3e-07 Leucine rich repeat
LRR_8 PF13855 200 - 257 3.9e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 705 - 770 8.8e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 872 - 1145 6.1e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 874 - 1090 3.9e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2042
AasI GACNNNNNNGTC 2 cut(s) 89, 2009
AatII GACGTC 3 cut(s) 126, 177, 704
Acc36I ACCTGC 1 cut(s) 2042
AccB1I GGYRCC 2 cut(s) 86, 3004
AccB7I CCANNNNNTGG 2 cut(s) 139, 1307
AccBSI CCGCTC 1 cut(s) 3002
AccI GTMKAC 1 cut(s) 78
AccIII TCCGGA 4 cut(s) 505, 741, 1244, 3206
AciI CCGC 7 cut(s) 359, 422, 1692, 1730, 1815, 3002, 3404
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 8 cut(s) 368, 745, 1107, 1474, 1657, 1670, 1774, 1839
AcuI CTGAAG 7 cut(s) 247, 275, 401, 831, 2574, 2697, 2996
AcyI GRCGYC 3 cut(s) 123, 174, 701
AfaI GTAC 9 cut(s) 761, 1638, 2253, 2569, 2618, 2740, 2768, 3020, 3391
AfeI AGCGCT 1 cut(s) 378
AfiI CCNNNNNNNGG 7 cut(s) 139, 299, 505, 1307, 2298, 2444, 3206
AhdI GACNNNNNGTC 1 cut(s) 3154
AjiI CACGTC 1 cut(s) 1736
AjnI CCWGG 5 cut(s) 138, 675, 961, 2277, 2728
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
Alw21I GWGCWC 2 cut(s) 396, 961
Alw26I GTCTC 4 cut(s) 130, 1978, 2330, 2907
AlwNI CAGNNNCTG 2 cut(s) 2393, 3344
Ama87I CYCGRG 1 cut(s) 2247
Aor13HI TCCGGA 4 cut(s) 505, 741, 1244, 3206
Aor51HI AGCGCT 1 cut(s) 378
AoxI GGCC 2 cut(s) 130, 1742
ApoI RAATTY 8 cut(s) 368, 745, 1107, 1474, 1657, 1670, 1774, 1839
AseI ATTAAT 1 cut(s) 2061
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 379, 3283
AspS9I GGNCC 2 cut(s) 1997, 2967
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 8 cut(s) 220, 1157, 1211, 1367, 1421, 1496, 1532, 2063
AsuII TTCGAA 1 cut(s) 1478
AvaI CYCGRG 1 cut(s) 2247
AvaII GGWCC 2 cut(s) 1997, 2967
AxyI CCTNAGG 1 cut(s) 642
BamHI GGATCC 2 cut(s) 1247, 1460
BanI GGYRCC 2 cut(s) 86, 3004
BanII GRGCYC 3 cut(s) 396, 961, 2710
BarI GAAGNNNNNNTAC 2 cut(s) 2600, 2632
BauI CACGAG 1 cut(s) 1791
BbsI GAAGAC 4 cut(s) 48, 254, 569, 1700
Bbv12I GWGCWC 2 cut(s) 396, 961
BbvCI CCTCAGC 1 cut(s) 1387
BceAI ACGGC 3 cut(s) 100, 1729, 1854
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 5 cut(s) 140, 677, 963, 2279, 2730
BciVI GTATCC 3 cut(s) 1183, 1393, 2267
BclI TGATCA 1 cut(s) 1630
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 4 cut(s) 130, 1978, 2330, 2907
BfaI CTAG 9 cut(s) 317, 663, 888, 911, 1329, 1346, 2043, 2165, 3474
BfmI CTRYAG 3 cut(s) 1053, 1131, 2172
BfoI RGCGCY 2 cut(s) 380, 3284
BfuAI ACCTGC 1 cut(s) 2042
BfuI GTATCC 3 cut(s) 1183, 1393, 2267
BlpI GCTNAGC 2 cut(s) 734, 865
Bme1390I CCNGG 6 cut(s) 140, 573, 677, 963, 2279, 2730
Bme18I GGWCC 2 cut(s) 1997, 2967
BmeRI GACNNNNNGTC 1 cut(s) 3154
BmeT110I CYCGRG 1 cut(s) 2247
BmgBI CACGTC 1 cut(s) 1736
BmgT120I GGNCC 2 cut(s) 1997, 2967
BmrFI CCNGG 6 cut(s) 140, 573, 677, 963, 2279, 2730
BmsI GCATC 9 cut(s) 544, 720, 956, 975, 987, 1436, 1717, 2344, 3155
BpiI GAAGAC 4 cut(s) 48, 254, 569, 1700
BpmI CTGGAG 5 cut(s) 410, 945, 1778, 3081, 3342
Bpu10I CCTNAGC 2 cut(s) 1387, 2626
Bpu1102I GCTNAGC 2 cut(s) 734, 865
Bpu14I TTCGAA 1 cut(s) 1478
BpuEI CTTGAG 4 cut(s) 437, 740, 1080, 1262
BpuMI CCSGG 1 cut(s) 573
BsaHI GRCGYC 3 cut(s) 123, 174, 701
BsaJI CCNNGG 1 cut(s) 399
BsaWI WCCGGW 5 cut(s) 230, 505, 741, 1244, 3206
BsaXI ACNNNNNCTCC 4 cut(s) 441, 471, 925, 955
Bsc4I CCNNNNNNNGG 7 cut(s) 139, 299, 505, 1307, 2298, 2444, 3206
Bse1I ACTGG 6 cut(s) 1392, 1761, 2312, 2883, 3144, 3305
Bse21I CCTNAGG 1 cut(s) 642
Bse3DI GCAATG 5 cut(s) 65, 1564, 1923, 2210, 2853
BseAI TCCGGA 4 cut(s) 505, 741, 1244, 3206
BseBI CCWGG 5 cut(s) 140, 677, 963, 2279, 2730
BseDI CCNNGG 1 cut(s) 399
BseLI CCNNNNNNNGG 7 cut(s) 139, 299, 505, 1307, 2298, 2444, 3206
BseMI GCAATG 5 cut(s) 65, 1564, 1923, 2210, 2853
BseMII CTCAG 8 cut(s) 633, 879, 1401, 2112, 2325, 3098, 3384, 3414
BseNI ACTGG 6 cut(s) 1392, 1761, 2312, 2883, 3144, 3305
BseRI GAGGAG 3 cut(s) 449, 3048, 3410
BseYI CCCAGC 1 cut(s) 2816
BsgI GTGCAG 3 cut(s) 347, 2204, 2859
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 132, 1744
BshNI GGYRCC 2 cut(s) 86, 3004
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 2 cut(s) 396, 961
BsiHKCI CYCGRG 1 cut(s) 2247
BslFI GGGAC 4 cut(s) 1746, 2599, 2816, 2980
BslI CCNNNNNNNGG 7 cut(s) 139, 299, 505, 1307, 2298, 2444, 3206
BsmAI GTCTC 4 cut(s) 130, 1978, 2330, 2907
BsmBI CGTCTC 2 cut(s) 130, 1978
BsmFI GGGAC 4 cut(s) 1746, 2599, 2816, 2980
BsmI GAATGC 4 cut(s) 326, 598, 2890, 3025
BsnI GGCC 2 cut(s) 132, 1744
BsoBI CYCGRG 1 cut(s) 2247
Bsp119I TTCGAA 1 cut(s) 1478
Bsp1286I GDGCHC 3 cut(s) 396, 961, 2710
Bsp13I TCCGGA 4 cut(s) 505, 741, 1244, 3206
Bsp1720I GCTNAGC 2 cut(s) 734, 865
BspACI CCGC 7 cut(s) 359, 422, 1692, 1730, 1815, 3002, 3404
BspANI GGCC 2 cut(s) 132, 1744
BspCNI CTCAG 8 cut(s) 634, 878, 1400, 2113, 2326, 3099, 3385, 3415
BspEI TCCGGA 4 cut(s) 505, 741, 1244, 3206
BspHI TCATGA 1 cut(s) 1645
BspMAI CTGCAG 1 cut(s) 1057
BspMI ACCTGC 1 cut(s) 2042
BspT104I TTCGAA 1 cut(s) 1478
BspT107I GGYRCC 2 cut(s) 86, 3004
BsrBI CCGCTC 1 cut(s) 3002
BsrDI GCAATG 5 cut(s) 65, 1564, 1923, 2210, 2853
BsrI ACTGG 6 cut(s) 1392, 1761, 2312, 2883, 3144, 3305
BssECI CCNNGG 1 cut(s) 399
BssNI GRCGYC 3 cut(s) 123, 174, 701
BssSI CACGAG 1 cut(s) 1791
BssT1I CCWWGG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 1791
Bst2UI CCWGG 5 cut(s) 140, 677, 963, 2279, 2730
Bst4CI ACNGT 8 cut(s) 91, 353, 1135, 1184, 1706, 2051, 2092, 2182
Bst6I CTCTTC 1 cut(s) 3015
BstACI GRCGYC 3 cut(s) 123, 174, 701
BstBI TTCGAA 1 cut(s) 1478
BstC8I GCNNGC 1 cut(s) 3458
BstH2I RGCGCY 2 cut(s) 380, 3284
BstHHI GCGC 2 cut(s) 379, 3283
BstMAI GTCTC 4 cut(s) 130, 1978, 2330, 2907
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 7 cut(s) 385, 694, 1498, 1554, 1750, 2730, 3317
BstNI CCWGG 5 cut(s) 140, 677, 963, 2279, 2730
BstNSI RCATGY 5 cut(s) 1268, 1496, 2361, 2831, 3460
BstSCI CCNGG 6 cut(s) 138, 571, 675, 961, 2277, 2728
BstSFI CTRYAG 3 cut(s) 1053, 1131, 2172
BstV2I GAAGAC 4 cut(s) 48, 254, 569, 1700
BstX2I RGATCY 7 cut(s) 181, 330, 541, 1247, 1273, 1460, 2389
BstXI CCANNNNNNTGG 1 cut(s) 1861
BstYI RGATCY 7 cut(s) 181, 330, 541, 1247, 1273, 1460, 2389
Bsu36I CCTNAGG 1 cut(s) 642
BsuI GTATCC 3 cut(s) 1183, 1393, 2267
BsuRI GGCC 2 cut(s) 132, 1744
BtrI CACGTC 1 cut(s) 1736
BtsIMutI CAGTG 6 cut(s) 1754, 1896, 2534, 2721, 2876, 3312
BveI ACCTGC 1 cut(s) 2042
Cac8I GCNNGC 1 cut(s) 3458
CaiI CAGNNNCTG 2 cut(s) 2393, 3344
CciI TCATGA 1 cut(s) 1645
CfoI GCGC 2 cut(s) 379, 3283
Cfr13I GGNCC 2 cut(s) 1997, 2967
CseI GACGC 3 cut(s) 259, 1995, 2012
Csp6I GTAC 9 cut(s) 760, 1637, 2252, 2568, 2617, 2739, 2767, 3019, 3390
CspCI CAANNNNNGTGG 2 cut(s) 2460, 2495
CviQI GTAC 9 cut(s) 760, 1637, 2252, 2568, 2617, 2739, 2767, 3019, 3390
DrdI GACNNNNNNGTC 2 cut(s) 89, 2009
DriI GACNNNNNGTC 1 cut(s) 3154
DseDI GACNNNNNNGTC 2 cut(s) 89, 2009
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 3015
Eam1105I GACNNNNNGTC 1 cut(s) 3154
EarI CTCTTC 1 cut(s) 3015
Ecl136II GAGCTC 2 cut(s) 394, 959
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 3 cut(s) 396, 961, 2710
Eco32I GATATC 2 cut(s) 2116, 2694
Eco47I GGWCC 2 cut(s) 1997, 2967
Eco47III AGCGCT 1 cut(s) 378
Eco53kI GAGCTC 2 cut(s) 394, 959
Eco57I CTGAAG 7 cut(s) 247, 275, 401, 831, 2574, 2697, 2996
Eco81I CCTNAGG 1 cut(s) 642
Eco88I CYCGRG 1 cut(s) 2247
EcoICRI GAGCTC 2 cut(s) 394, 959
EcoO109I RGGNCCY 1 cut(s) 2967
EcoRI GAATTC 1 cut(s) 1474
EcoRII CCWGG 5 cut(s) 138, 675, 961, 2277, 2728
EcoRV GATATC 2 cut(s) 2116, 2694
EcoT14I CCWWGG 1 cut(s) 399
EcoT22I ATGCAT 3 cut(s) 326, 2359, 3120
EcoT38I GRGCYC 3 cut(s) 396, 961, 2710
ErhI CCWWGG 1 cut(s) 399
Esp3I CGTCTC 2 cut(s) 130, 1978
FalI AAGNNNNNCTT 2 cut(s) 2424, 2456
FaqI GGGAC 4 cut(s) 1746, 2599, 2816, 2980
FauI CCCGC 1 cut(s) 1723
FbaI TGATCA 1 cut(s) 1630
FblI GTMKAC 1 cut(s) 78
FriOI GRGCYC 3 cut(s) 396, 961, 2710
FspBI CTAG 9 cut(s) 317, 663, 888, 911, 1329, 1346, 2043, 2165, 3474
GlaI GCGC 2 cut(s) 378, 3282
GsaI CCCAGC 1 cut(s) 2820
GsuI CTGGAG 5 cut(s) 410, 945, 1778, 3081, 3342
HaeII RGCGCY 2 cut(s) 380, 3284
HaeIII GGCC 2 cut(s) 132, 1744
HgaI GACGC 3 cut(s) 259, 1995, 2012
HhaI GCGC 2 cut(s) 379, 3283
Hin1I GRCGYC 3 cut(s) 123, 174, 701
Hin6I GCGC 2 cut(s) 377, 3281
HinP1I GCGC 2 cut(s) 377, 3281
HincII GTYRAC 7 cut(s) 79, 1123, 2326, 3148, 3186, 3264, 3373
HindII GTYRAC 7 cut(s) 79, 1123, 2326, 3148, 3186, 3264, 3373
HpaI GTTAAC 2 cut(s) 1123, 3264
HphI GGTGA 8 cut(s) 220, 1157, 1211, 1367, 1421, 1496, 1532, 2063
Hpy166II GTNNAC 8 cut(s) 79, 1123, 2054, 2326, 3148, 3186, 3264, 3373
Hpy8I GTNNAC 8 cut(s) 79, 1123, 2054, 2326, 3148, 3186, 3264, 3373
HpyAV CCTTC 9 cut(s) 376, 509, 801, 1000, 1076, 1136, 1252, 2721, 3179
HpyCH4III ACNGT 8 cut(s) 91, 353, 1135, 1184, 1706, 2051, 2092, 2182
HpyCH4IV ACGT 6 cut(s) 123, 174, 701, 1624, 1682, 1735
HpyF10VI GCNNNNNNNGC 7 cut(s) 385, 694, 1498, 1554, 1750, 2730, 3317
HpySE526I ACGT 6 cut(s) 123, 174, 701, 1624, 1682, 1735
Hsp92I GRCGYC 3 cut(s) 123, 174, 701
HspAI GCGC 2 cut(s) 377, 3281
KflI GGGWCCC 1 cut(s) 2967
Kpn2I TCCGGA 4 cut(s) 505, 741, 1244, 3206
Ksp22I TGATCA 1 cut(s) 1630
KspAI GTTAAC 2 cut(s) 1123, 3264
LweI GCATC 9 cut(s) 544, 720, 956, 975, 987, 1436, 1717, 2344, 3155
MaeI CTAG 9 cut(s) 317, 663, 888, 911, 1329, 1346, 2043, 2165, 3474
MaeII ACGT 6 cut(s) 123, 174, 701, 1624, 1682, 1735
MaeIII GTNAC 4 cut(s) 403, 1042, 2792, 3241
MbiI CCGCTC 1 cut(s) 3002
MflI RGATCY 7 cut(s) 181, 330, 541, 1247, 1273, 1460, 2389
MhlI GDGCHC 3 cut(s) 396, 961, 2710
MlyI GAGTC 8 cut(s) 104, 444, 842, 1781, 2321, 2332, 3350, 3418
MmeI TCCRAC 9 cut(s) 648, 837, 899, 906, 1812, 2324, 3055, 3255, 3440
Mph1103I ATGCAT 3 cut(s) 326, 2359, 3120
MroI TCCGGA 4 cut(s) 505, 741, 1244, 3206
MroXI GAANNNNTTC 1 cut(s) 260
MslI CAYNNNNRTG 5 cut(s) 412, 995, 1644, 2701, 3163
MspA1I CMGCKG 5 cut(s) 361, 1815, 2960, 3320, 3422
MspR9I CCNGG 6 cut(s) 140, 573, 677, 963, 2279, 2730
Mva1269I GAATGC 4 cut(s) 326, 598, 2890, 3025
MvaI CCWGG 5 cut(s) 140, 677, 963, 2279, 2730
MwoI GCNNNNNNNGC 7 cut(s) 385, 694, 1498, 1554, 1750, 2730, 3317
NciI CCSGG 1 cut(s) 573
NmuCI GTSAC 2 cut(s) 403, 3241
NsiI ATGCAT 3 cut(s) 326, 2359, 3120
NspI RCATGY 5 cut(s) 1268, 1496, 2361, 2831, 3460
NspV TTCGAA 1 cut(s) 1478
PaeI GCATGC 1 cut(s) 3460
PaeR7I CTCGAG 1 cut(s) 2247
PagI TCATGA 1 cut(s) 1645
PaqCI CACCTGC 1 cut(s) 2042
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 90, 105
PctI GAATGC 4 cut(s) 326, 598, 2890, 3025
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 5 cut(s) 59, 751, 1049, 1297, 3400
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 2 cut(s) 139, 1307
PfoI TCCNGGA 1 cut(s) 961
Ple19I CGATCG 1 cut(s) 153
PleI GAGTC 8 cut(s) 104, 444, 841, 1781, 2321, 2332, 3349, 3417
PpsI GAGTC 8 cut(s) 104, 444, 841, 1781, 2321, 2332, 3349, 3417
PpuMI RGGWCCY 1 cut(s) 2967
PshBI ATTAAT 1 cut(s) 2061
Psp124BI GAGCTC 2 cut(s) 396, 961
Psp5II RGGWCCY 1 cut(s) 2967
Psp6I CCWGG 5 cut(s) 138, 675, 961, 2277, 2728
PspFI CCCAGC 1 cut(s) 2816
PspGI CCWGG 5 cut(s) 138, 675, 961, 2277, 2728
PspPI GGNCC 2 cut(s) 1997, 2967
PspPPI RGGWCCY 1 cut(s) 2967
PspXI VCTCGAGB 1 cut(s) 2247
PstI CTGCAG 1 cut(s) 1057
PstNI CAGNNNCTG 2 cut(s) 2393, 3344
PsuI RGATCY 7 cut(s) 181, 330, 541, 1247, 1273, 1460, 2389
PsyI GACNNNGTC 1 cut(s) 173
PvuI CGATCG 1 cut(s) 153
PvuII CAGCTG 3 cut(s) 2960, 3320, 3422
RsaI GTAC 9 cut(s) 761, 1638, 2253, 2569, 2618, 2740, 2768, 3020, 3391
RsaNI GTAC 9 cut(s) 760, 1637, 2252, 2568, 2617, 2739, 2767, 3019, 3390
RseI CAYNNNNRTG 5 cut(s) 412, 995, 1644, 2701, 3163
SacI GAGCTC 2 cut(s) 396, 961
SalI GTCGAC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 1997, 2967
SchI GAGTC 8 cut(s) 104, 444, 842, 1781, 2321, 2332, 3350, 3418
ScrFI CCNGG 6 cut(s) 140, 573, 677, 963, 2279, 2730
SduI GDGCHC 3 cut(s) 396, 961, 2710
SfaNI GCATC 9 cut(s) 544, 720, 956, 975, 987, 1436, 1717, 2344, 3155
SfcI CTRYAG 3 cut(s) 1053, 1131, 2172
Sfr274I CTCGAG 1 cut(s) 2247
SfuI TTCGAA 1 cut(s) 1478
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 2 cut(s) 1997, 2967
SlaI CTCGAG 1 cut(s) 2247
SmiMI CAYNNNNRTG 5 cut(s) 412, 995, 1644, 2701, 3163
SmlI CTYRAG 5 cut(s) 452, 755, 1095, 1277, 2247
SmoI CTYRAG 5 cut(s) 452, 755, 1095, 1277, 2247
SphI GCATGC 1 cut(s) 3460
SsiI CCGC 7 cut(s) 359, 422, 1692, 1730, 1815, 3002, 3404
SspI AATATT 3 cut(s) 2305, 2399, 3130
SspMI CTAG 9 cut(s) 317, 663, 888, 911, 1329, 1346, 2043, 2165, 3474
SstI GAGCTC 2 cut(s) 396, 961
StyD4I CCNGG 6 cut(s) 138, 571, 675, 961, 2277, 2728
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 8 cut(s) 91, 353, 1135, 1184, 1706, 2051, 2092, 2182
TaiI ACGT 6 cut(s) 126, 177, 704, 1627, 1685, 1738
TatI WGTACW 5 cut(s) 759, 1636, 2616, 2766, 3389
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 5 cut(s) 59, 751, 1049, 1297, 3400
TscAI CASTG 6 cut(s) 1761, 1903, 2534, 2721, 2883, 3312
TseFI GTSAC 2 cut(s) 403, 3241
Tsp45I GTSAC 2 cut(s) 403, 3241
TspGWI ACGGA 3 cut(s) 7, 1996, 2440
TspRI CASTG 6 cut(s) 1761, 1903, 2534, 2721, 2883, 3312
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 2 cut(s) 139, 1307
VpaK11BI GGWCC 2 cut(s) 1997, 2967
VspI ATTAAT 1 cut(s) 2061
XapI RAATTY 8 cut(s) 368, 745, 1107, 1474, 1657, 1670, 1774, 1839
XceI RCATGY 5 cut(s) 1268, 1496, 2361, 2831, 3460
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XhoI CTCGAG 1 cut(s) 2247
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 9 cut(s) 317, 663, 888, 911, 1329, 1346, 2043, 2165, 3474
ZraI GACGTC 3 cut(s) 124, 175, 702
Zsp2I ATGCAT 3 cut(s) 326, 2359, 3120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.