Rh4AG357300

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014727
Physical Location & Seq
Reverse (-)
66771103 .. 66775230
4128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG357300.1

Sequence Viewer

Length: 3492 bp
ATGGAAGCCCCAATTTCCGTCGAAAACCCTAAAACCTCCGAAGACCCAGAAACTTCCGATTCCACATTGCAAGTCGTCGACGACGACGACGGCACCGTCGCCGGAGCCGGAGCCGGAGCCGACTCGATCCTCGACGTCTCCGGCCAAAGTCTGGACTTCCCGATCGGCGAGAACTCCGGCGACGACGTCGGGGATCTATACCTCTACAAAAATGTGTACAATTTACTGCCTAAATCCATCACCGGACTAAAAGGCTTGAAGACGCTGAAGTTCTTCAGCAACGAGATCAATCTGTTCTCGTCGCCGGAGATCGGCGACATGGCAAGGCTAGAATGCATGAGGATCTCGTCGCCGGAGTTCGACGGTTTGCCGCTGCACAAATTCAAAGCGCTGAAGGAGCTGGAGCTCTCCAAGGTCACATCTCGGTGCTTGGCGGTTCCCATTTTGAGCCAGATCGCCGGACTCAAGCTCCTCACGAAGCTATCGGTATGTTATTTCTCTATAACTTTCCTTCCTCCGGAGATTTGCTGCTTAAAGAGTTTGGAGCATCTGGATCTTTCGTTCAACAAGATGAAGACCTTGCCGGGAGAAATTGGCAATTTGAATGCTCTGGTGTCGTTGAGAGTTGCCAATAATAAATTGGAGGAATTGCCTGAGGGTTTGTCCAGATTAGCTAGGTTGGAGAACCTGGACTTGTCGCATAATAGGCTGACGTCATTGGGGGTTGTTGATTTTGGGTTGATGCTTAGCCTCCGGAATTTGAATCTCAAGTACAATAGGCTTTCGAGTGAGTGTGTAGTGCCTTCGTGGATATGCTGCGATTTGGATGGGAATGGAAATGATGAGTCTGGCAGTTCTTCAGTCGAGTTGGATTGCTCAGCCAATGTAATTAGTGTGTCTAGTAAATCGAGAAGTAGGTTTCTAGCAAGTTGGAGGTCGGATGGGAGGAAGTGTTATTTTCGGTGGAGAGCTCCAGGATGCGTAAATAAAGGCAGGATGCCCATGCCGATGCGTGTGAAGGAAGATGGAGAAAGCAAAACAGAAAATGTAGATGTTACTGATTCTGCAGTAGATGAGGATGAGGATAAGGATGAAGGTTTAGTATCTCAAGAAGCTGAAATTTGTTTGTTTGTTAACTCTACTACAGTAGATGAAGGTGATAAAAAAGATTGTGAGGTTGAAGTATCCTCAAACAGTCAAGAGGTTGCTGGTGAGCAAGATGAAGCATTATGGTCAGAAATGTTAAAAGCCACTTCCGGATCCAAGAGGAAGGAGCATGTTGGGGATCTCAAGAAGCCGAATTGCAAGAATCCAAAATCTGGTGGGATTAGTGGTGTATCTAGTTATGATATTTCACTAGATCAAGGTGATAAAAACGATTACGAGAATGAAGTATCCTCAGCCAGTCAAGAGGTTGTCGGTGAGAAAGATGAAGAAGCATCATGTTTAGAAAAATCAGAAACTACTTTGGGATCCAAGAGGAAGAATTCGAATGGTGATGAGCATGTTAAGCAGCCAGATTGTGAGAATGGTGATGGGAATGTTAAGCAGCCAGACTGCAAGAATGGCAATGGGGATGTTAAGCAGCCAGATTGCAAGAATGGTGTTGGGGATAATAAGCAGCACAAGAGATTACGTCTTGATCAAGTACATAGTCATGATTGTTCAAATTTATCTCAAAAATTTAGTGATACGTCTTTTTGCGGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCGGGACGTGATAGGCCGTTCATGCCACTGGAGAGTTATGATGAAAATTTCCCTCTTGACTCTCGTGAAGTTATTTTCTTGGACAGCGGAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGTGGTTCGCCGTTTGAATGAGTTAAATGATAATCGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTCTTTGTGTCAGATCATTTTGGAGGGAGTGATAGCGTTGCTGGTGTTGAACGGAGACGCAAAGGTGGTCCTGACGCAAATGTCAGGAAGCCTTTTGTATGCACCTGCTCAACTAGAAACGGTGAACCTATTAATTTATCCACTAAACCATACTTGCAGACGGTTGAAGTTAATGCTTTTGTTGATATCTCTGAGAAATCTCTCCGCTCTATTAAAGCAAGACATAAATCTATCCTAGTTCCTATAGGAACTGTGCAGTATGGTGTTTGCAGACATAGAGCATTGCTATTGAAGTATCTATGTGATCGGATGGAGCCTCGAGTACCTTGTGAGCTTGTTAGGGGATACCTGGATTTCATGCCCCATGCTTGGAATATTATACCAGTCAAGAGAGTTGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCGAATGACATGAGAAAGGAAACAGATCCTGAATATTATTGTAGGTATATTCCTATGACACGGATCAAAGTTCCTCTTTCTGGAAGCAACTTTGGTTGTTCTTTGCCACATTCTTTGCTCTCTTTTGATGGAACTCTGAACAAATCTGGCAGTTCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTAGCAGCAAAAATGCGTACTTTGGAGGTAGATGGGACTTCAGGGGATGATGTTAGGAAGTTCGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCACCACCCCTGCATAGTAGAGTTGTATGGACATCAGATATCATCCAAGTGGGCTCCTTCAGTGAATGGCACTCCTGGCTCCCGTACTTTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCTTAAAGAGTTACATAGAAAAGCTATCAAAAGCTGGTGAGAAGCATGTCCCTGTGCATTTAGCTGTTTGCATTGCTAAAAATGTTGCTTCTGCACTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACATCAAAAGTGAGAACATTTTGATTGATTTGAATAGCAAGACAGCTGATGGGACCCCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTTCAGTCGTACTTGCATTCCTGCCTTGTCGCTCATGTAGGAATACCTCCTCCTAATGTTTGCGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGAGAACAATGCATGAGCAAAATATTTATGGACTGGAAGTTGACATCTGGTCATTTGGATGCCTTCTTTCCGAATTGTTGACTTTGCAACTTCCTTACTCCGGAGTAGCTGATTTGGAGATACATGACCTTATTGTGACAGGACAACGACCTAAGTTAACAGATGAGTTGGAGGCGCTTCTGCTATATATGGACGAGCCAGTGGCTCGATCAGCTGGAGAGCTTGGGGGAACAGAGGCTGACTTAGACACACTAACATTTCTTGTTGACTTGTTCTGCAAATGTACTGAGGAGAATCCGCAGAGTCGTCCAACAGCTGAGAACATCTATAAATTGTTGCTCGAACACAGCATGCAGCTTACAAACTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1163

Amino Acids

128.6

Weight (kDa)

5.11

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 123 - 262 1.9e-14 Leucine-rich repeat region
LRR_8 PF13855 158 - 214 2.3e-07 Leucine rich repeat
LRR_8 PF13855 204 - 261 3.9e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 706 - 774 2.9e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 876 - 1149 6.4e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 878 - 1094 3.6e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2054
AasI GACNNNNNNGTC 2 cut(s) 95, 2021
AatII GACGTC 3 cut(s) 138, 189, 716
Acc36I ACCTGC 1 cut(s) 2054
AccB1I GGYRCC 2 cut(s) 92, 3016
AccB7I CCANNNNNTGG 2 cut(s) 151, 1319
AccBSI CCGCTC 2 cut(s) 2148, 3014
AccI GTMKAC 1 cut(s) 78
AccIII TCCGGA 4 cut(s) 517, 753, 1256, 3218
AciI CCGC 8 cut(s) 371, 434, 1704, 1742, 1827, 2146, 3014, 3416
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 8 cut(s) 380, 757, 1119, 1486, 1669, 1682, 1786, 1851
AcuI CTGAAG 7 cut(s) 259, 287, 413, 843, 2586, 2709, 3008
AcyI GRCGYC 3 cut(s) 135, 186, 713
AfeI AGCGCT 1 cut(s) 390
AfiI CCNNNNNNNGG 7 cut(s) 151, 311, 517, 1319, 2310, 2456, 3218
AhdI GACNNNNNGTC 1 cut(s) 3166
AjiI CACGTC 1 cut(s) 1748
AjnI CCWGG 4 cut(s) 687, 973, 2289, 2740
AloI GAACNNNNNNTCC 2 cut(s) 545, 577
Alw21I GWGCWC 2 cut(s) 408, 973
Alw26I GTCTC 4 cut(s) 142, 1990, 2342, 2919
AlwNI CAGNNNCTG 2 cut(s) 2405, 3356
Ama87I CYCGRG 1 cut(s) 2259
Aor13HI TCCGGA 4 cut(s) 517, 753, 1256, 3218
Aor51HI AGCGCT 1 cut(s) 390
AoxI GGCC 2 cut(s) 142, 1754
ApoI RAATTY 8 cut(s) 380, 757, 1119, 1486, 1669, 1682, 1786, 1851
AseI ATTAAT 1 cut(s) 2073
Asp700I GAANNNNTTC 1 cut(s) 272
AspLEI GCGC 2 cut(s) 391, 3295
AspS9I GGNCC 2 cut(s) 2009, 2979
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 9 cut(s) 232, 1169, 1223, 1379, 1433, 1508, 1544, 2075, 2843
AsuII TTCGAA 1 cut(s) 1490
AvaI CYCGRG 1 cut(s) 2259
AvaII GGWCC 2 cut(s) 2009, 2979
AxyI CCTNAGG 1 cut(s) 654
BamHI GGATCC 2 cut(s) 1259, 1472
BanI GGYRCC 2 cut(s) 92, 3016
BanII GRGCYC 3 cut(s) 408, 973, 2722
BarI GAAGNNNNNNTAC 2 cut(s) 2612, 2644
BauI CACGAG 1 cut(s) 1803
BbsI GAAGAC 4 cut(s) 48, 266, 581, 1712
Bbv12I GWGCWC 2 cut(s) 408, 973
BbvCI CCTCAGC 1 cut(s) 1399
BceAI ACGGC 3 cut(s) 106, 1741, 1866
BciT130I CCWGG 4 cut(s) 689, 975, 2291, 2742
BciVI GTATCC 3 cut(s) 1195, 1405, 2279
BclI TGATCA 1 cut(s) 1642
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 4 cut(s) 142, 1990, 2342, 2919
BfaI CTAG 9 cut(s) 329, 675, 900, 923, 1341, 1358, 2055, 2177, 3486
BfmI CTRYAG 3 cut(s) 1065, 1143, 2184
BfoI RGCGCY 2 cut(s) 392, 3296
BfuAI ACCTGC 1 cut(s) 2054
BfuI GTATCC 3 cut(s) 1195, 1405, 2279
BlpI GCTNAGC 2 cut(s) 746, 877
Bme1390I CCNGG 5 cut(s) 585, 689, 975, 2291, 2742
Bme18I GGWCC 2 cut(s) 2009, 2979
BmeRI GACNNNNNGTC 1 cut(s) 3166
BmeT110I CYCGRG 1 cut(s) 2259
BmgBI CACGTC 1 cut(s) 1748
BmgT120I GGNCC 2 cut(s) 2009, 2979
BmrFI CCNGG 5 cut(s) 585, 689, 975, 2291, 2742
BmsI GCATC 9 cut(s) 556, 732, 968, 987, 999, 1448, 1729, 2356, 3167
BpiI GAAGAC 4 cut(s) 48, 266, 581, 1712
BpmI CTGGAG 5 cut(s) 422, 957, 1790, 3093, 3354
Bpu10I CCTNAGC 2 cut(s) 1399, 2638
Bpu1102I GCTNAGC 2 cut(s) 746, 877
Bpu14I TTCGAA 1 cut(s) 1490
BpuEI CTTGAG 4 cut(s) 449, 752, 1092, 1274
BpuMI CCSGG 1 cut(s) 585
BsaHI GRCGYC 3 cut(s) 135, 186, 713
BsaJI CCNNGG 1 cut(s) 411
BsaWI WCCGGW 5 cut(s) 242, 517, 753, 1256, 3218
BsaXI ACNNNNNCTCC 4 cut(s) 453, 483, 937, 967
Bsc4I CCNNNNNNNGG 7 cut(s) 151, 311, 517, 1319, 2310, 2456, 3218
Bse1I ACTGG 6 cut(s) 1404, 1773, 2324, 2895, 3156, 3317
Bse21I CCTNAGG 1 cut(s) 654
Bse3DI GCAATG 5 cut(s) 65, 1576, 1935, 2222, 2865
BseAI TCCGGA 4 cut(s) 517, 753, 1256, 3218
BseBI CCWGG 4 cut(s) 689, 975, 2291, 2742
BseDI CCNNGG 1 cut(s) 411
BseLI CCNNNNNNNGG 7 cut(s) 151, 311, 517, 1319, 2310, 2456, 3218
BseMI GCAATG 5 cut(s) 65, 1576, 1935, 2222, 2865
BseMII CTCAG 8 cut(s) 645, 891, 1413, 2124, 2337, 3110, 3396, 3426
BseNI ACTGG 6 cut(s) 1404, 1773, 2324, 2895, 3156, 3317
BseRI GAGGAG 3 cut(s) 461, 3060, 3422
BsgI GTGCAG 3 cut(s) 359, 2216, 2871
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 2 cut(s) 144, 1756
BshNI GGYRCC 2 cut(s) 92, 3016
BsiEI CGRYCG 1 cut(s) 165
BsiHKAI GWGCWC 2 cut(s) 408, 973
BsiHKCI CYCGRG 1 cut(s) 2259
BslFI GGGAC 4 cut(s) 1758, 2611, 2828, 2992
BslI CCNNNNNNNGG 7 cut(s) 151, 311, 517, 1319, 2310, 2456, 3218
BsmAI GTCTC 4 cut(s) 142, 1990, 2342, 2919
BsmBI CGTCTC 2 cut(s) 142, 1990
BsmFI GGGAC 4 cut(s) 1758, 2611, 2828, 2992
BsmI GAATGC 4 cut(s) 338, 610, 2902, 3037
BsnI GGCC 2 cut(s) 144, 1756
BsoBI CYCGRG 1 cut(s) 2259
Bsp119I TTCGAA 1 cut(s) 1490
Bsp1286I GDGCHC 3 cut(s) 408, 973, 2722
Bsp13I TCCGGA 4 cut(s) 517, 753, 1256, 3218
Bsp1407I TGTACA 1 cut(s) 216
Bsp1720I GCTNAGC 2 cut(s) 746, 877
BspACI CCGC 8 cut(s) 371, 434, 1704, 1742, 1827, 2146, 3014, 3416
BspANI GGCC 2 cut(s) 144, 1756
BspCNI CTCAG 8 cut(s) 646, 890, 1412, 2125, 2338, 3111, 3397, 3427
BspEI TCCGGA 4 cut(s) 517, 753, 1256, 3218
BspHI TCATGA 1 cut(s) 1657
BspMAI CTGCAG 1 cut(s) 1069
BspMI ACCTGC 1 cut(s) 2054
BspT104I TTCGAA 1 cut(s) 1490
BspT107I GGYRCC 2 cut(s) 92, 3016
BsrBI CCGCTC 2 cut(s) 2148, 3014
BsrDI GCAATG 5 cut(s) 65, 1576, 1935, 2222, 2865
BsrGI TGTACA 1 cut(s) 216
BsrI ACTGG 6 cut(s) 1404, 1773, 2324, 2895, 3156, 3317
BssECI CCNNGG 1 cut(s) 411
BssNI GRCGYC 3 cut(s) 135, 186, 713
BssSI CACGAG 1 cut(s) 1803
BssT1I CCWWGG 1 cut(s) 411
Bst2BI CACGAG 1 cut(s) 1803
Bst2UI CCWGG 4 cut(s) 689, 975, 2291, 2742
Bst4CI ACNGT 8 cut(s) 97, 365, 1147, 1196, 1718, 2063, 2104, 2194
Bst6I CTCTTC 1 cut(s) 3027
BstACI GRCGYC 3 cut(s) 135, 186, 713
BstAUI TGTACA 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 1490
BstC8I GCNNGC 1 cut(s) 3470
BstH2I RGCGCY 2 cut(s) 392, 3296
BstHHI GCGC 2 cut(s) 391, 3295
BstMAI GTCTC 4 cut(s) 142, 1990, 2342, 2919
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 7 cut(s) 397, 706, 1510, 1566, 1762, 2742, 3329
BstNI CCWGG 4 cut(s) 689, 975, 2291, 2742
BstNSI RCATGY 5 cut(s) 1280, 1508, 2373, 2843, 3472
BstSCI CCNGG 5 cut(s) 583, 687, 973, 2289, 2740
BstSFI CTRYAG 3 cut(s) 1065, 1143, 2184
BstV2I GAAGAC 4 cut(s) 48, 266, 581, 1712
BstX2I RGATCY 7 cut(s) 193, 342, 553, 1259, 1285, 1472, 2401
BstXI CCANNNNNNTGG 1 cut(s) 1873
BstYI RGATCY 7 cut(s) 193, 342, 553, 1259, 1285, 1472, 2401
Bsu36I CCTNAGG 1 cut(s) 654
BsuI GTATCC 3 cut(s) 1195, 1405, 2279
BsuRI GGCC 2 cut(s) 144, 1756
BtrI CACGTC 1 cut(s) 1748
BtsIMutI CAGTG 6 cut(s) 1766, 1908, 2546, 2733, 2888, 3324
BveI ACCTGC 1 cut(s) 2054
Cac8I GCNNGC 1 cut(s) 3470
CaiI CAGNNNCTG 2 cut(s) 2405, 3356
CciI TCATGA 1 cut(s) 1657
CfoI GCGC 2 cut(s) 391, 3295
Cfr13I GGNCC 2 cut(s) 2009, 2979
CseI GACGC 3 cut(s) 271, 2007, 2024
CspCI CAANNNNNGTGG 2 cut(s) 2472, 2507
DrdI GACNNNNNNGTC 2 cut(s) 95, 2021
DriI GACNNNNNGTC 1 cut(s) 3166
DseDI GACNNNNNNGTC 2 cut(s) 95, 2021
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 3027
Eam1105I GACNNNNNGTC 1 cut(s) 3166
EarI CTCTTC 1 cut(s) 3027
Ecl136II GAGCTC 2 cut(s) 406, 971
Eco130I CCWWGG 1 cut(s) 411
Eco24I GRGCYC 3 cut(s) 408, 973, 2722
Eco32I GATATC 2 cut(s) 2128, 2706
Eco47I GGWCC 2 cut(s) 2009, 2979
Eco47III AGCGCT 1 cut(s) 390
Eco53kI GAGCTC 2 cut(s) 406, 971
Eco57I CTGAAG 7 cut(s) 259, 287, 413, 843, 2586, 2709, 3008
Eco81I CCTNAGG 1 cut(s) 654
Eco88I CYCGRG 1 cut(s) 2259
EcoICRI GAGCTC 2 cut(s) 406, 971
EcoO109I RGGNCCY 1 cut(s) 2979
EcoRI GAATTC 1 cut(s) 1486
EcoRII CCWGG 4 cut(s) 687, 973, 2289, 2740
EcoRV GATATC 2 cut(s) 2128, 2706
EcoT14I CCWWGG 1 cut(s) 411
EcoT22I ATGCAT 3 cut(s) 338, 2371, 3132
EcoT38I GRGCYC 3 cut(s) 408, 973, 2722
ErhI CCWWGG 1 cut(s) 411
Esp3I CGTCTC 2 cut(s) 142, 1990
FalI AAGNNNNNCTT 2 cut(s) 2436, 2468
FaqI GGGAC 4 cut(s) 1758, 2611, 2828, 2992
FauI CCCGC 1 cut(s) 1735
FbaI TGATCA 1 cut(s) 1642
FblI GTMKAC 1 cut(s) 78
FriOI GRGCYC 3 cut(s) 408, 973, 2722
FspBI CTAG 9 cut(s) 329, 675, 900, 923, 1341, 1358, 2055, 2177, 3486
GlaI GCGC 2 cut(s) 390, 3294
GsuI CTGGAG 5 cut(s) 422, 957, 1790, 3093, 3354
HaeII RGCGCY 2 cut(s) 392, 3296
HaeIII GGCC 2 cut(s) 144, 1756
HgaI GACGC 3 cut(s) 271, 2007, 2024
HhaI GCGC 2 cut(s) 391, 3295
Hin1I GRCGYC 3 cut(s) 135, 186, 713
Hin6I GCGC 2 cut(s) 389, 3293
HinP1I GCGC 2 cut(s) 389, 3293
HincII GTYRAC 7 cut(s) 79, 1135, 2338, 3160, 3198, 3276, 3385
HindII GTYRAC 7 cut(s) 79, 1135, 2338, 3160, 3198, 3276, 3385
HpaI GTTAAC 2 cut(s) 1135, 3276
HphI GGTGA 9 cut(s) 232, 1169, 1223, 1379, 1433, 1508, 1544, 2075, 2843
Hpy166II GTNNAC 9 cut(s) 79, 217, 1135, 2066, 2338, 3160, 3198, 3276, 3385
Hpy8I GTNNAC 9 cut(s) 79, 217, 1135, 2066, 2338, 3160, 3198, 3276, 3385
HpyAV CCTTC 9 cut(s) 388, 521, 813, 1012, 1088, 1148, 1264, 2733, 3191
HpyCH4III ACNGT 8 cut(s) 97, 365, 1147, 1196, 1718, 2063, 2104, 2194
HpyCH4IV ACGT 6 cut(s) 135, 186, 713, 1636, 1694, 1747
HpyF10VI GCNNNNNNNGC 7 cut(s) 397, 706, 1510, 1566, 1762, 2742, 3329
HpySE526I ACGT 6 cut(s) 135, 186, 713, 1636, 1694, 1747
Hsp92I GRCGYC 3 cut(s) 135, 186, 713
HspAI GCGC 2 cut(s) 389, 3293
KflI GGGWCCC 1 cut(s) 2979
Kpn2I TCCGGA 4 cut(s) 517, 753, 1256, 3218
Ksp22I TGATCA 1 cut(s) 1642
KspAI GTTAAC 2 cut(s) 1135, 3276
LweI GCATC 9 cut(s) 556, 732, 968, 987, 999, 1448, 1729, 2356, 3167
MaeI CTAG 9 cut(s) 329, 675, 900, 923, 1341, 1358, 2055, 2177, 3486
MaeII ACGT 6 cut(s) 135, 186, 713, 1636, 1694, 1747
MaeIII GTNAC 4 cut(s) 415, 1054, 2804, 3253
MbiI CCGCTC 2 cut(s) 2148, 3014
MflI RGATCY 7 cut(s) 193, 342, 553, 1259, 1285, 1472, 2401
MhlI GDGCHC 3 cut(s) 408, 973, 2722
MlyI GAGTC 7 cut(s) 116, 456, 854, 1793, 2333, 2344, 3430
MmeI TCCRAC 9 cut(s) 660, 849, 911, 918, 1824, 2336, 3067, 3267, 3452
Mph1103I ATGCAT 3 cut(s) 338, 2371, 3132
MroI TCCGGA 4 cut(s) 517, 753, 1256, 3218
MroXI GAANNNNTTC 1 cut(s) 272
MslI CAYNNNNRTG 5 cut(s) 424, 1007, 1656, 2713, 3175
MspA1I CMGCKG 5 cut(s) 373, 1827, 2972, 3332, 3434
MspR9I CCNGG 5 cut(s) 585, 689, 975, 2291, 2742
Mva1269I GAATGC 4 cut(s) 338, 610, 2902, 3037
MvaI CCWGG 4 cut(s) 689, 975, 2291, 2742
MwoI GCNNNNNNNGC 7 cut(s) 397, 706, 1510, 1566, 1762, 2742, 3329
NciI CCSGG 1 cut(s) 585
NmuCI GTSAC 2 cut(s) 415, 3253
NsiI ATGCAT 3 cut(s) 338, 2371, 3132
NspI RCATGY 5 cut(s) 1280, 1508, 2373, 2843, 3472
NspV TTCGAA 1 cut(s) 1490
PaeI GCATGC 1 cut(s) 3472
PaeR7I CTCGAG 1 cut(s) 2259
PagI TCATGA 1 cut(s) 1657
PaqCI CACCTGC 1 cut(s) 2054
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 84, 93
PctI GAATGC 4 cut(s) 338, 610, 2902, 3037
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 5 cut(s) 59, 763, 1061, 1309, 3412
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 2 cut(s) 151, 1319
PfoI TCCNGGA 1 cut(s) 973
Ple19I CGATCG 1 cut(s) 165
PleI GAGTC 7 cut(s) 116, 456, 853, 1793, 2333, 2344, 3429
PpsI GAGTC 7 cut(s) 116, 456, 853, 1793, 2333, 2344, 3429
PpuMI RGGWCCY 1 cut(s) 2979
PshBI ATTAAT 1 cut(s) 2073
Psp124BI GAGCTC 2 cut(s) 408, 973
Psp5II RGGWCCY 1 cut(s) 2979
Psp6I CCWGG 4 cut(s) 687, 973, 2289, 2740
PspGI CCWGG 4 cut(s) 687, 973, 2289, 2740
PspPI GGNCC 2 cut(s) 2009, 2979
PspPPI RGGWCCY 1 cut(s) 2979
PspXI VCTCGAGB 1 cut(s) 2259
PstI CTGCAG 1 cut(s) 1069
PstNI CAGNNNCTG 2 cut(s) 2405, 3356
PsuI RGATCY 7 cut(s) 193, 342, 553, 1259, 1285, 1472, 2401
PsyI GACNNNGTC 1 cut(s) 185
PvuI CGATCG 1 cut(s) 165
PvuII CAGCTG 3 cut(s) 2972, 3332, 3434
RseI CAYNNNNRTG 5 cut(s) 424, 1007, 1656, 2713, 3175
SacI GAGCTC 2 cut(s) 408, 973
SalI GTCGAC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 2009, 2979
SchI GAGTC 7 cut(s) 116, 456, 854, 1793, 2333, 2344, 3430
ScrFI CCNGG 5 cut(s) 585, 689, 975, 2291, 2742
SduI GDGCHC 3 cut(s) 408, 973, 2722
SfaNI GCATC 9 cut(s) 556, 732, 968, 987, 999, 1448, 1729, 2356, 3167
SfcI CTRYAG 3 cut(s) 1065, 1143, 2184
Sfr274I CTCGAG 1 cut(s) 2259
SfuI TTCGAA 1 cut(s) 1490
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 2 cut(s) 2009, 2979
SlaI CTCGAG 1 cut(s) 2259
SmiMI CAYNNNNRTG 5 cut(s) 424, 1007, 1656, 2713, 3175
SmlI CTYRAG 5 cut(s) 464, 767, 1107, 1289, 2259
SmoI CTYRAG 5 cut(s) 464, 767, 1107, 1289, 2259
SphI GCATGC 1 cut(s) 3472
SsiI CCGC 8 cut(s) 371, 434, 1704, 1742, 1827, 2146, 3014, 3416
SspI AATATT 3 cut(s) 2317, 2411, 3142
SspMI CTAG 9 cut(s) 329, 675, 900, 923, 1341, 1358, 2055, 2177, 3486
SstI GAGCTC 2 cut(s) 408, 973
StyD4I CCNGG 5 cut(s) 583, 687, 973, 2289, 2740
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 8 cut(s) 97, 365, 1147, 1196, 1718, 2063, 2104, 2194
TaiI ACGT 6 cut(s) 138, 189, 716, 1639, 1697, 1750
TatI WGTACW 6 cut(s) 216, 771, 1648, 2628, 2778, 3401
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 5 cut(s) 59, 763, 1061, 1309, 3412
TscAI CASTG 6 cut(s) 1773, 1915, 2546, 2733, 2895, 3324
TseFI GTSAC 2 cut(s) 415, 3253
Tsp45I GTSAC 2 cut(s) 415, 3253
TspGWI ACGGA 3 cut(s) 7, 2008, 2452
TspRI CASTG 6 cut(s) 1773, 1915, 2546, 2733, 2895, 3324
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 2 cut(s) 151, 1319
VpaK11BI GGWCC 2 cut(s) 2009, 2979
VspI ATTAAT 1 cut(s) 2073
XapI RAATTY 8 cut(s) 380, 757, 1119, 1486, 1669, 1682, 1786, 1851
XceI RCATGY 5 cut(s) 1280, 1508, 2373, 2843, 3472
XcmI CCANNNNNNNNNTGG 1 cut(s) 637
XhoI CTCGAG 1 cut(s) 2259
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 9 cut(s) 329, 675, 900, 923, 1341, 1358, 2055, 2177, 3486
ZraI GACGTC 3 cut(s) 136, 187, 714
Zsp2I ATGCAT 3 cut(s) 338, 2371, 3132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.