Rh4BG368200

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014727
Physical Location & Seq
Reverse (-)
52671627 .. 52675606
3980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG368200.1

Sequence Viewer

Length: 3477 bp
ATGGAAGCCCCAATTTCCGTCGAAAACCCTAAAACCTCCGAAGACCCCGAAACCTCCGATTCCACATTGCAAGTCGTCGACGACGGTGCCGTCGTCGGAGCCGGAGCCGACTCGATCCTCGACGTCTCCGGCCAAAGCCTGGACTTCCCGATCGGCGAGAACTCCGGCGACGACGTCGGGGATCTATACCTCTACAAAAATGTGTACAATTTACTGCCTAAATCCATCGCCGGACTAAAAGGCTTGAAGACGCTGAAGTTCTTCAGCAACGAGATCAATCTGTTCTCGTCGCCGGAGATCGGCGACATGGCAAGGCTAGAATGCATGAGGATCTCCTCGCCGGAGTTCGACGGTTTGCCGCTGCACAAATTCAAAGCGCTGAAGGAGCTGGAGCTCTCCAAGGTCACATCTCGGTGCTCGGCGGTTCCGATTTTGAACCAGATCGCCGGACTCAAGCTCCTCACGAAGCTATCGGTATGTTATTTCTCTATAACTTTTCTTCCTCCGGAGATTTGCTGCTTAAAGAGTTTGGAGCATCTGGATCTTTCGTTCAACAAGATGAAGACTTTACCGGGAGAAATTGGCAATTTGAATGCTCTGGTGTCGTTGAGAGTTGCCAATAATAAATTGGAGGAATTGCCTGAGGGTTTGTCCAGATTAGCTAGGCTGGAGAACCTGGACTTGTCGCATAATAGGCTGACGTCATTGGGGGTTGTTGATTTTGGGTTGATGCTGAGCCTCCAGAATTTGAATCTCAAGTACAATAGGCTTTCGAGTGAGTGTGTAGTGCCTTCGTGGATATGCTGCGATTTGGATGGGAATGGAAATGATGTGTCTGGCAGTTCTTCAGTCGAGTTGGATTGCTCAGCGAATGTAATTAGTGTGTCTAGTAAATCGAGAAGTAGGTTTCTAGCAAGTTGGAGGTCGGATGGGAGGAAGTGTTATTTTCGGTGGAGAGCTCCAGGATGCGTAAATAAAGGCAGGGTGCCCATGCCGATGCGTGTGGAGGAAGATGGAGAAAGCAAAACAGAAAATGTAGATGTTACTGATTCTGCAGTAGATGAGGATGAGGATGAAGGTTTAGTATCTCGAGAAGCTGAAATTTGTTCGTTTGTTATCTCTACTGCAGTAGATGAAGGTGATAAAAAAGATTGTGAGGTTGAAGTATCCTCAAACAGTCAAGAGGTTGCTGGTGAGCAAGATGAAGCATTATGGTCAGAAATGTTAAAAGCCACTTCCGGATCCAAGAGGAAGGAGCATGTTGGGGATCTCAAGAAGCCGAATTGCAAGAATCCAAAATCTGGTGGGATTAGTGGTGTATCTAGTTATGATATTTCACTAGATCAAGGTGATAAAAACAATTACGAGAATGAAGTATCCTCCTCAGCCAGTCAAGAGGTTGTCGGTGAGAAAGATGAAGAAGCATCATGTTTAGAAAAATCAGAAACTACTTTGGGATCCAAGAGGAAGAATTCAAATGGTGATGAGGATGTTAAGCAGCCAGATTGCGGGAATGGTGATGGGAATGTTAAGCAGCCAGACTGCAAGAATGGTGATGGGGATGTTAAGCAGCCAGATTGCAAGAATGGTGTTGGGGATATTAAGCAGCACAAGAGATTACGTCTTGATCAAGTACATAGTCATGATTGTTCAAATTTATCTCAAAAATTTAGTGATACGTCTTTTTGCAGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCGGGACGTGATAGGCCGTTCATGCCACTGGAGAGTTATGATGAAAATTTCCCTCTTGACTCTCGTGAAGTTATTTTCTTGGACAGCGGAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGTGGTTCACCGTTTGAATGAGTTAAATGATAATCGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTCTTTGTGTCAGATCATTTTGGAGGGAGTGATAGGGTTGCTGGTGTTGAACGGAGACGCAAAGGTGGTCCTGACGCAAATGTCAGGAAGCCTTTTGTATGCACCTGCTCAACTAGAAACGGTGAACCTATTAATTTATCCACCAAACCATACTTGCAGACGGTTGAAGATAATGCTTTTGTTGATATCTCTGAGAAATCTCTCCGCTCTATTAAAGCAAGACATAAATCTATCCTAGTTCCTATAGGAACTGTGCAGTATGGTGTTTGCAGACATAGAGCATTGCTATTGAAGTATCTATGTGATCGGATGGAGCCTCGAGTACCTTGTGAGCTTGTTAGGGGATACCTGGATTTCATGCCCCATGCTTGGAATATTATACCAGTCAAGAGAGTTGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCAAATGACATGAGAAAGGAAACAGATCCTGAATATTATTGTAGGTATATTCCTATGACACGGATCAAAGTTCCTCTTTCTGGAAGCAACTTTGGTTGTTCTTTGCCACATTCTTTGCCCTCTTTTGATGGAACTCTGAACAAATCTGGCAGTTCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTAGCAGCAAAAATGCGTACTTTGGAGGTAGATGGGACTTCAGGGGATGATGTTAGGAAGTTCGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCACCACCCCTGCATAGTAGAGTTGTATGGACATCAGATATCATCCAAGTGGGCTCCTTCAGTGAATGGCACTCCCGGCTCCCGTACTTTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCTTAAAGAGTTACATAGAAAAGCTATCAAAAGCTGGTGAGAAGCATGTCCCTGTGCATTTAGCTTTTTGCATTGCTAAAAATGTTGCTTCTGCACTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACATCAAAAGTGAGAACATTTTGATTGATTTGAATAGCAAGACAGCTGATGGGACCCCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTTCAGTCGTACTTGCATTCCTGCCTTGTCGCTCATGTAGGAATACCTCCTCCTAATGTTTGCGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGAGAACAATGCATGAGCAAAATATTTATGGACTGGAAGTTGACATCTGGTCGTTTGGATGCCTTCTTTCCGAATTGTTGACTTTGCAACTTCCTTACTCGGGAGTAGCTGATTTGGAGATACATGACCTTATTGTGACGGGACAACGACCTAAGTTAACAGATGAGTTGGAGGCGCTTCTGCTATTTATGGACGAGCCAGTGGCTCGATCAGCTGGAGAGCTTGGGGGAACAGAGGCTGACTTAGACACACTAACATTTCTTGCTGACTTGTTCTGCAAATGTACTGAGGAGAATCCGCAGAGTCGTCCAACAGCTGAGAACATCTACAAATTGTTGCTCGAACACAGCATGCAGCTTACAAACTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1158

Amino Acids

127.93

Weight (kDa)

5.1

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 116 - 257 3.1e-14 Leucine-rich repeat region
LRR_8 PF13855 154 - 210 2.4e-07 Leucine rich repeat
LRR_8 PF13855 200 - 257 5.5e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 698 - 769 1.4e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 871 - 1144 1.2e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 873 - 1089 7.2e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2039
AasI GACNNNNNNGTC 2 cut(s) 89, 2006
AatII GACGTC 3 cut(s) 126, 177, 704
Acc36I ACCTGC 1 cut(s) 2039
AccB1I GGYRCC 3 cut(s) 86, 985, 3001
AccB7I CCANNNNNTGG 2 cut(s) 139, 1301
AccBSI CCGCTC 2 cut(s) 2133, 2999
AccI GTMKAC 1 cut(s) 78
AccIII TCCGGA 2 cut(s) 505, 1238
AciI CCGC 8 cut(s) 359, 422, 1509, 1727, 1812, 2131, 2999, 3401
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 8 cut(s) 368, 745, 1101, 1471, 1654, 1667, 1771, 1836
AcuI CTGAAG 7 cut(s) 247, 275, 401, 831, 2571, 2694, 2993
AcyI GRCGYC 3 cut(s) 123, 174, 701
AfeI AGCGCT 1 cut(s) 378
AfiI CCNNNNNNNGG 7 cut(s) 139, 299, 1301, 1508, 2295, 2441, 3203
AhdI GACNNNNNGTC 1 cut(s) 3151
AjiI CACGTC 1 cut(s) 1733
AjnI CCWGG 4 cut(s) 138, 675, 961, 2274
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
Alw21I GWGCWC 3 cut(s) 396, 419, 961
Alw26I GTCTC 4 cut(s) 130, 1975, 2327, 2904
AlwNI CAGNNNCTG 2 cut(s) 2390, 3341
Ama87I CYCGRG 3 cut(s) 1089, 2244, 3202
Aor13HI TCCGGA 2 cut(s) 505, 1238
Aor51HI AGCGCT 1 cut(s) 378
AoxI GGCC 2 cut(s) 130, 1739
ApoI RAATTY 8 cut(s) 368, 745, 1101, 1471, 1654, 1667, 1771, 1836
AseI ATTAAT 1 cut(s) 2058
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 379, 3280
AspS9I GGNCC 2 cut(s) 1994, 2964
AsuC2I CCSGG 2 cut(s) 573, 2727
AvaI CYCGRG 3 cut(s) 1089, 2244, 3202
AvaII GGWCC 2 cut(s) 1994, 2964
AxyI CCTNAGG 1 cut(s) 642
BaeGI GKGCMC 1 cut(s) 990
BamHI GGATCC 2 cut(s) 1241, 1457
BanI GGYRCC 3 cut(s) 86, 985, 3001
BanII GRGCYC 3 cut(s) 396, 961, 2707
BarI GAAGNNNNNNTAC 2 cut(s) 2597, 2629
BauI CACGAG 1 cut(s) 1788
BbsI GAAGAC 4 cut(s) 48, 254, 569, 1697
Bbv12I GWGCWC 3 cut(s) 396, 419, 961
BbvCI CCTCAGC 1 cut(s) 1384
BceAI ACGGC 2 cut(s) 74, 1726
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 4 cut(s) 140, 677, 963, 2276
BciVI GTATCC 3 cut(s) 1177, 1387, 2264
BclI TGATCA 1 cut(s) 1627
BcnI CCSGG 2 cut(s) 573, 2727
BcoDI GTCTC 4 cut(s) 130, 1975, 2327, 2904
BfaI CTAG 9 cut(s) 317, 663, 888, 911, 1323, 1340, 2040, 2162, 3471
BfmI CTRYAG 3 cut(s) 1053, 1125, 2169
BfoI RGCGCY 2 cut(s) 380, 3281
BfuAI ACCTGC 1 cut(s) 2039
BfuI GTATCC 3 cut(s) 1177, 1387, 2264
BlpI GCTNAGC 2 cut(s) 734, 865
Bme1390I CCNGG 6 cut(s) 140, 573, 677, 963, 2276, 2727
Bme18I GGWCC 2 cut(s) 1994, 2964
BmeRI GACNNNNNGTC 1 cut(s) 3151
BmeT110I CYCGRG 3 cut(s) 1089, 2244, 3202
BmgBI CACGTC 1 cut(s) 1733
BmgT120I GGNCC 2 cut(s) 1994, 2964
BmrFI CCNGG 6 cut(s) 140, 573, 677, 963, 2276, 2727
BmsI GCATC 8 cut(s) 544, 720, 956, 987, 1433, 1714, 2341, 3152
BpiI GAAGAC 4 cut(s) 48, 254, 569, 1697
BpmI CTGGAG 7 cut(s) 410, 689, 725, 945, 1775, 3078, 3339
Bpu10I CCTNAGC 2 cut(s) 1384, 2623
Bpu1102I GCTNAGC 2 cut(s) 734, 865
BpuEI CTTGAG 3 cut(s) 437, 740, 1256
BpuMI CCSGG 2 cut(s) 573, 2727
BsaHI GRCGYC 3 cut(s) 123, 174, 701
BsaJI CCNNGG 1 cut(s) 399
BsaWI WCCGGW 2 cut(s) 505, 1238
BsaXI ACNNNNNCTCC 4 cut(s) 441, 471, 925, 955
Bsc4I CCNNNNNNNGG 7 cut(s) 139, 299, 1301, 1508, 2295, 2441, 3203
Bse1I ACTGG 6 cut(s) 1389, 1758, 2309, 2880, 3141, 3302
Bse21I CCTNAGG 1 cut(s) 642
Bse3DI GCAATG 4 cut(s) 65, 1920, 2207, 2850
BseAI TCCGGA 2 cut(s) 505, 1238
BseBI CCWGG 4 cut(s) 140, 677, 963, 2276
BseDI CCNNGG 1 cut(s) 399
BseLI CCNNNNNNNGG 7 cut(s) 139, 299, 1301, 1508, 2295, 2441, 3203
BseMI GCAATG 4 cut(s) 65, 1920, 2207, 2850
BseMII CTCAG 9 cut(s) 633, 725, 879, 1398, 2109, 2322, 3095, 3381, 3411
BseNI ACTGG 6 cut(s) 1389, 1758, 2309, 2880, 3141, 3302
BseRI GAGGAG 5 cut(s) 325, 449, 1372, 3045, 3407
BseSI GKGCMC 1 cut(s) 990
BsgI GTGCAG 3 cut(s) 347, 2201, 2856
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 132, 1741
BshNI GGYRCC 3 cut(s) 86, 985, 3001
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 3 cut(s) 396, 419, 961
BsiHKCI CYCGRG 3 cut(s) 1089, 2244, 3202
BslFI GGGAC 5 cut(s) 1743, 2596, 2813, 2977, 3258
BslI CCNNNNNNNGG 7 cut(s) 139, 299, 1301, 1508, 2295, 2441, 3203
BsmAI GTCTC 4 cut(s) 130, 1975, 2327, 2904
BsmBI CGTCTC 2 cut(s) 130, 1975
BsmFI GGGAC 5 cut(s) 1743, 2596, 2813, 2977, 3258
BsmI GAATGC 4 cut(s) 326, 598, 2887, 3022
BsnI GGCC 2 cut(s) 132, 1741
BsoBI CYCGRG 3 cut(s) 1089, 2244, 3202
Bsp1286I GDGCHC 5 cut(s) 396, 419, 961, 990, 2707
Bsp13I TCCGGA 2 cut(s) 505, 1238
Bsp1407I TGTACA 1 cut(s) 204
Bsp1720I GCTNAGC 2 cut(s) 734, 865
BspACI CCGC 8 cut(s) 359, 422, 1509, 1727, 1812, 2131, 2999, 3401
BspANI GGCC 2 cut(s) 132, 1741
BspCNI CTCAG 9 cut(s) 634, 726, 878, 1397, 2110, 2323, 3096, 3382, 3412
BspEI TCCGGA 2 cut(s) 505, 1238
BspHI TCATGA 1 cut(s) 1642
BspMAI CTGCAG 2 cut(s) 1057, 1129
BspMI ACCTGC 1 cut(s) 2039
BspT107I GGYRCC 3 cut(s) 86, 985, 3001
BsrBI CCGCTC 2 cut(s) 2133, 2999
BsrDI GCAATG 4 cut(s) 65, 1920, 2207, 2850
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 6 cut(s) 1389, 1758, 2309, 2880, 3141, 3302
BssECI CCNNGG 1 cut(s) 399
BssNI GRCGYC 3 cut(s) 123, 174, 701
BssSI CACGAG 1 cut(s) 1788
BssT1I CCWWGG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 1788
Bst2UI CCWGG 4 cut(s) 140, 677, 963, 2276
Bst4CI ACNGT 8 cut(s) 86, 353, 1178, 1703, 1868, 2048, 2089, 2179
Bst6I CTCTTC 1 cut(s) 3012
BstACI GRCGYC 3 cut(s) 123, 174, 701
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 3455
BstH2I RGCGCY 2 cut(s) 380, 3281
BstHHI GCGC 2 cut(s) 379, 3280
BstMAI GTCTC 4 cut(s) 130, 1975, 2327, 2904
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 5 cut(s) 385, 694, 1747, 2727, 3314
BstNI CCWGG 4 cut(s) 140, 677, 963, 2276
BstNSI RCATGY 4 cut(s) 1262, 2358, 2828, 3457
BstSCI CCNGG 6 cut(s) 138, 571, 675, 961, 2274, 2725
BstSFI CTRYAG 3 cut(s) 1053, 1125, 2169
BstSLI GKGCMC 1 cut(s) 990
BstV2I GAAGAC 4 cut(s) 48, 254, 569, 1697
BstX2I RGATCY 7 cut(s) 181, 330, 541, 1241, 1267, 1457, 2386
BstXI CCANNNNNNTGG 1 cut(s) 1858
BstYI RGATCY 7 cut(s) 181, 330, 541, 1241, 1267, 1457, 2386
Bsu36I CCTNAGG 1 cut(s) 642
BsuI GTATCC 3 cut(s) 1177, 1387, 2264
BsuRI GGCC 2 cut(s) 132, 1741
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 1733
BtsIMutI CAGTG 6 cut(s) 1751, 1893, 2531, 2718, 2873, 3309
BveI ACCTGC 1 cut(s) 2039
Cac8I GCNNGC 1 cut(s) 3455
CaiI CAGNNNCTG 2 cut(s) 2390, 3341
CciI TCATGA 1 cut(s) 1642
CfoI GCGC 2 cut(s) 379, 3280
Cfr13I GGNCC 2 cut(s) 1994, 2964
CseI GACGC 3 cut(s) 259, 1992, 2009
CspCI CAANNNNNGTGG 2 cut(s) 2457, 2492
DrdI GACNNNNNNGTC 2 cut(s) 89, 2006
DriI GACNNNNNGTC 1 cut(s) 3151
DseDI GACNNNNNNGTC 2 cut(s) 89, 2006
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 3012
Eam1105I GACNNNNNGTC 1 cut(s) 3151
EarI CTCTTC 1 cut(s) 3012
Ecl136II GAGCTC 2 cut(s) 394, 959
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 3 cut(s) 396, 961, 2707
Eco32I GATATC 2 cut(s) 2113, 2691
Eco47I GGWCC 2 cut(s) 1994, 2964
Eco47III AGCGCT 1 cut(s) 378
Eco53kI GAGCTC 2 cut(s) 394, 959
Eco57I CTGAAG 7 cut(s) 247, 275, 401, 831, 2571, 2694, 2993
Eco81I CCTNAGG 1 cut(s) 642
Eco88I CYCGRG 3 cut(s) 1089, 2244, 3202
EcoICRI GAGCTC 2 cut(s) 394, 959
EcoO109I RGGNCCY 1 cut(s) 2964
EcoRI GAATTC 1 cut(s) 1471
EcoRII CCWGG 4 cut(s) 138, 675, 961, 2274
EcoRV GATATC 2 cut(s) 2113, 2691
EcoT14I CCWWGG 1 cut(s) 399
EcoT22I ATGCAT 3 cut(s) 326, 2356, 3117
EcoT38I GRGCYC 3 cut(s) 396, 961, 2707
ErhI CCWWGG 1 cut(s) 399
Esp3I CGTCTC 2 cut(s) 130, 1975
FalI AAGNNNNNCTT 2 cut(s) 2421, 2453
FaqI GGGAC 5 cut(s) 1743, 2596, 2813, 2977, 3258
FauI CCCGC 2 cut(s) 1502, 1720
FbaI TGATCA 1 cut(s) 1627
FblI GTMKAC 1 cut(s) 78
FriOI GRGCYC 3 cut(s) 396, 961, 2707
FspBI CTAG 9 cut(s) 317, 663, 888, 911, 1323, 1340, 2040, 2162, 3471
GlaI GCGC 2 cut(s) 378, 3279
GsuI CTGGAG 7 cut(s) 410, 689, 725, 945, 1775, 3078, 3339
HaeII RGCGCY 2 cut(s) 380, 3281
HaeIII GGCC 2 cut(s) 132, 1741
HgaI GACGC 3 cut(s) 259, 1992, 2009
HhaI GCGC 2 cut(s) 379, 3280
Hin1I GRCGYC 3 cut(s) 123, 174, 701
Hin6I GCGC 2 cut(s) 377, 3278
HinP1I GCGC 2 cut(s) 377, 3278
HincII GTYRAC 5 cut(s) 79, 2323, 3145, 3183, 3261
HindII GTYRAC 5 cut(s) 79, 2323, 3145, 3183, 3261
HpaI GTTAAC 1 cut(s) 3261
Hpy166II GTNNAC 8 cut(s) 79, 205, 1864, 2051, 2323, 3145, 3183, 3261
Hpy8I GTNNAC 8 cut(s) 79, 205, 1864, 2051, 2323, 3145, 3183, 3261
HpyAV CCTTC 7 cut(s) 376, 801, 1070, 1130, 1246, 2718, 3176
HpyCH4III ACNGT 8 cut(s) 86, 353, 1178, 1703, 1868, 2048, 2089, 2179
HpyCH4IV ACGT 6 cut(s) 123, 174, 701, 1621, 1679, 1732
HpyF10VI GCNNNNNNNGC 5 cut(s) 385, 694, 1747, 2727, 3314
HpySE526I ACGT 6 cut(s) 123, 174, 701, 1621, 1679, 1732
Hsp92I GRCGYC 3 cut(s) 123, 174, 701
HspAI GCGC 2 cut(s) 377, 3278
KflI GGGWCCC 1 cut(s) 2964
Kpn2I TCCGGA 2 cut(s) 505, 1238
Ksp22I TGATCA 1 cut(s) 1627
KspAI GTTAAC 1 cut(s) 3261
LweI GCATC 8 cut(s) 544, 720, 956, 987, 1433, 1714, 2341, 3152
MaeI CTAG 9 cut(s) 317, 663, 888, 911, 1323, 1340, 2040, 2162, 3471
MaeII ACGT 6 cut(s) 123, 174, 701, 1621, 1679, 1732
MaeIII GTNAC 4 cut(s) 403, 1042, 2789, 3238
MbiI CCGCTC 2 cut(s) 2133, 2999
MflI RGATCY 7 cut(s) 181, 330, 541, 1241, 1267, 1457, 2386
MhlI GDGCHC 5 cut(s) 396, 419, 961, 990, 2707
MlyI GAGTC 6 cut(s) 104, 444, 1778, 2318, 2329, 3415
MmeI TCCRAC 9 cut(s) 76, 837, 899, 906, 1809, 2321, 3052, 3252, 3437
Mph1103I ATGCAT 3 cut(s) 326, 2356, 3117
MroI TCCGGA 2 cut(s) 505, 1238
MroXI GAANNNNTTC 1 cut(s) 260
MslI CAYNNNNRTG 4 cut(s) 412, 995, 1641, 2698
MspA1I CMGCKG 5 cut(s) 361, 1812, 2957, 3317, 3419
MspR9I CCNGG 6 cut(s) 140, 573, 677, 963, 2276, 2727
Mva1269I GAATGC 4 cut(s) 326, 598, 2887, 3022
MvaI CCWGG 4 cut(s) 140, 677, 963, 2276
MwoI GCNNNNNNNGC 5 cut(s) 385, 694, 1747, 2727, 3314
NciI CCSGG 2 cut(s) 573, 2727
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 2 cut(s) 403, 3238
NsiI ATGCAT 3 cut(s) 326, 2356, 3117
NspI RCATGY 4 cut(s) 1262, 2358, 2828, 3457
PaeI GCATGC 1 cut(s) 3457
PaeR7I CTCGAG 2 cut(s) 1089, 2244
PagI TCATGA 1 cut(s) 1642
PaqCI CACCTGC 1 cut(s) 2039
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 90, 425
PctI GAATGC 4 cut(s) 326, 598, 2887, 3022
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 5 cut(s) 59, 751, 1049, 1291, 3397
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 2 cut(s) 139, 1301
PfoI TCCNGGA 1 cut(s) 961
Ple19I CGATCG 1 cut(s) 153
PleI GAGTC 6 cut(s) 104, 444, 1778, 2318, 2329, 3414
PpsI GAGTC 6 cut(s) 104, 444, 1778, 2318, 2329, 3414
PpuMI RGGWCCY 1 cut(s) 2964
PshBI ATTAAT 1 cut(s) 2058
Psp124BI GAGCTC 2 cut(s) 396, 961
Psp5II RGGWCCY 1 cut(s) 2964
Psp6I CCWGG 4 cut(s) 138, 675, 961, 2274
PspGI CCWGG 4 cut(s) 138, 675, 961, 2274
PspPI GGNCC 2 cut(s) 1994, 2964
PspPPI RGGWCCY 1 cut(s) 2964
PspXI VCTCGAGB 1 cut(s) 2244
PstI CTGCAG 2 cut(s) 1057, 1129
PstNI CAGNNNCTG 2 cut(s) 2390, 3341
PsuI RGATCY 7 cut(s) 181, 330, 541, 1241, 1267, 1457, 2386
PsyI GACNNNGTC 1 cut(s) 173
PvuI CGATCG 1 cut(s) 153
PvuII CAGCTG 3 cut(s) 2957, 3317, 3419
RseI CAYNNNNRTG 4 cut(s) 412, 995, 1641, 2698
SacI GAGCTC 2 cut(s) 396, 961
SalI GTCGAC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 1994, 2964
SchI GAGTC 6 cut(s) 104, 444, 1778, 2318, 2329, 3415
ScrFI CCNGG 6 cut(s) 140, 573, 677, 963, 2276, 2727
SduI GDGCHC 5 cut(s) 396, 419, 961, 990, 2707
SfaNI GCATC 8 cut(s) 544, 720, 956, 987, 1433, 1714, 2341, 3152
SfcI CTRYAG 3 cut(s) 1053, 1125, 2169
Sfr274I CTCGAG 2 cut(s) 1089, 2244
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 2 cut(s) 1994, 2964
SlaI CTCGAG 2 cut(s) 1089, 2244
SmiMI CAYNNNNRTG 4 cut(s) 412, 995, 1641, 2698
SmlI CTYRAG 5 cut(s) 452, 755, 1089, 1271, 2244
SmoI CTYRAG 5 cut(s) 452, 755, 1089, 1271, 2244
SphI GCATGC 1 cut(s) 3457
SsiI CCGC 8 cut(s) 359, 422, 1509, 1727, 1812, 2131, 2999, 3401
SspI AATATT 3 cut(s) 2302, 2396, 3127
SspMI CTAG 9 cut(s) 317, 663, 888, 911, 1323, 1340, 2040, 2162, 3471
SstI GAGCTC 2 cut(s) 396, 961
StyD4I CCNGG 6 cut(s) 138, 571, 675, 961, 2274, 2725
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 8 cut(s) 86, 353, 1178, 1703, 1868, 2048, 2089, 2179
TaiI ACGT 6 cut(s) 126, 177, 704, 1624, 1682, 1735
TatI WGTACW 6 cut(s) 204, 759, 1633, 2613, 2763, 3386
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 5 cut(s) 59, 751, 1049, 1291, 3397
TscAI CASTG 6 cut(s) 1758, 1900, 2531, 2718, 2880, 3309
TseFI GTSAC 2 cut(s) 403, 3238
Tsp45I GTSAC 2 cut(s) 403, 3238
TspGWI ACGGA 3 cut(s) 7, 1993, 2437
TspRI CASTG 6 cut(s) 1758, 1900, 2531, 2718, 2880, 3309
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 2 cut(s) 139, 1301
VpaK11BI GGWCC 2 cut(s) 1994, 2964
VspI ATTAAT 1 cut(s) 2058
XapI RAATTY 8 cut(s) 368, 745, 1101, 1471, 1654, 1667, 1771, 1836
XceI RCATGY 4 cut(s) 1262, 2358, 2828, 3457
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XhoI CTCGAG 2 cut(s) 1089, 2244
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 9 cut(s) 317, 663, 888, 911, 1323, 1340, 2040, 2162, 3471
ZraI GACGTC 3 cut(s) 124, 175, 702
Zsp2I ATGCAT 3 cut(s) 326, 2356, 3117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.