Rh4DG349200

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014727
Physical Location & Seq
Forward (+)
57072641 .. 57076748
4108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG349200.1

Sequence Viewer

Length: 3474 bp
ATGGAAGCCCCAATTTCCGTCGAAAACCCTAAAACCTCCGAAGACCCAGAAACTTCCGATTCCACATTGCAAGTCGTCGACGACGGTGCCGTCGTCGTCGCCGGAGCCGGAGCCGACTCGATCCTCGACGTCTCCGGCCAAAGCCTGGACTTCCCGATCGGCGAGAACTCCGGCGACGATGTCGGGGATCTATACCTCTACAAAAATGTGTACAATTTACTGCCTAAATCCATCACCGGACTAAAAGGCTTGAAGACGCTGAAGTTCTTCGGCAACGAGATCAATCTGTTCTCGTCGCCGGAGATCGGCGACATGGCAAGGCTAGAATGCATGAGGATCTCGTCGCCGGAGTTCGACGGTTTGCCGCTGCACAAATTCAAAGCGCTGAAGGAGCTGGAGCTCTCCAAGGTCACATCTCGGTGCCTGGCGGTTCCCATTTTGAGCCAGATCGCCGGACTCAAGCTCCTCACGAAGCTATCGGTATGTTATTTCTCTATAACTTTCCTTCCTCCGGAGATTTGCTGCTTAAAGAGTTTGGAGCATCTGGATCTTTCGTTCAACAAGATGAAGACTTTACCGGGAGAAATTGGCAATTTGAATGCTCTGGTGTCGTTGAGAGTTGCCAATAATAAATTGGAGGAATTGCCTGAGGGTTTGTCCAGATTAGCTAGGTTGGAGAACCTGGACTTGTCGCATAATAGGCTGACGTCATTGGGGGTTGTTGATTTTGGGTTGATGCTTAGCCTCCGGAATTTGAATCTCAAGTACAATAGGCTTTCGAGTGAGTGTGTAGTGCCTCCGTGGATATGCTGCGATTTGGATGGGAATGGAAATGATGAGTCTGGCAGTTCTTCAGTCGAGTTGGATTGCTCAGCCAATGTAATTAGTGTGTCTAGTAAATCGAGAAGTAGGTTTCTAGCAAGTTGGAGGTCGGATGGGAGGAAGTGTTATTTTCGGTGGAGAGCTCCAGGATGCGTAAATAAAGGCAGGATGCCCATGCCAATGCGTGTGAAGGAAGATGGAGAAAGCAAAACAGAAAATGTAGATGTTACTGATTCTGCAGTAGATGAGGATGAAGGTTTAGTATCTCGAGAAGCTGAAATTTGTTGGTTTGTTAACTCTGCTGCAGTAGATGAAGGTGATAAAAAAGATTGTGAGGTTGAAGTATCCTCAAACAGTCAAGAGGTTGCTGGTGAGCAAGATGAAGCATTATGGTCAGAAATGTTAAAAGCCACTTCCGGATCCAAGAGGAAGGAGCATGTTGGGGATCTCAAGAAGCCGAATTGCAAGAATCCAAAATCTGGTGGGATTAGTGGTGTATCTAGTTATGATATTTCACTAGATCAAGGTGATAAAAACGATTACGAGAATGAAGTATCCTCAGCCAGTCAAGAGGTTGTCGGTGAGAAAGATGAAGAAGCATCATGTTTAGAAAAATCAGAAACTACTTTGGGATCCAAGAGGAAGAATTCGAATGGTGATGAGCATGTTAAGCAGCCAGATTGTGAGAATGGTGATGGGAATGTTAAGCAGCCAGACTGCAAGAATGGCAATGGGGATGTTAAGCAGCCAGATTGCAAGAATGGTGTTGGGGATAATAAGCAGCACAAGAGATTACGTCTTGATCAAGTACATAGTCATGATTGTTCAAATTTATCTCAAAAATTTAGTGATACGTCTTTTTGCGGTATTGAGGACAGTCTTCAAGATGGCTTTTATGATGCGGGACGCGATAGGCCGTTCATGCCACTGGAGAGTTATGATGAAAATTTCCCTCTTGACTCTCGTGAAGTTATTTTCTTGGACAGCGGAAAGGATGATGAGTTGGATGGAATTTTTCAGTCTGCCAGAGATGTGGTTCGCCGTTTGAATGAGTTAAATGATAATCGCACTGATGAGCGACAGATTGCAATGTTTCTTGCTCTCTTTGTGTCAGATCATTTTGGAGGAAGTGATAGGGTTGCTGGTGTTGAACGGAGACGCAAAGGTGGTCCTGACACAAATGTCAGGAAGCCTTTTGTATGCACCTGCTCAACTAGAAACGGTGAACCTATTAATTTATCCACCAAACCATACTTGCAGGCGGTTGAAGATAATGCTTTTGTTGATATCTCTGAGAAATCTCTCCGCTCTATTAAAGCAAGACATAAATCTATCCTAGTTCCTATAGGAACTGTGCAGTATGGTGTTTGCAGACATAGAGCATTGCTATTGAAGTATCTATGTGATCGGATGGAGCCTCGAGTACCTTGTGAGCTTGTTAGGGGATACCTGGATTTCATGCCCCATGCTTGGAATATTATACCAGTCAAGAGAGTTGACTCAACTGAGACTCGTTGGATAGTTGATGCATGTCGTCCGAATGATATGAGAAAGGAAACAGATCCTGAATATTATTGTAGGTATATTCCTATGACACGGATCAAAGTTCCTCTTTCTGGAAGCAACTTTGGTTGTTCTTTGCCACATTCTTTGCCCTCTTTTGATGGAACTCTGAACAAATCTGGCAGTTCTCTCATTCAGTGCAAATATGGATCAGTTGAGTTAGCAGCAAAAATGCGTACTTTGGAGGTAGATGGGACTTCAGGGGATGATGTTAGGAAGTTTGAGTACAACTGCTTAGGGGAAGTAAGAATGTTAAGTGCTTTGCACCACCCCTGCATAGTAGAGTTGTATGGACATCAGATATCATCCAAGTGGGCTCCTTCAGTGAATGGCACTCCTGGCTCCCGTACTTTGCAATCAGCAATTTTCATGGAGTACATAAATGGGGGTTCCTTAAAGAGTTACATAGAAAAGCTATCAAAAGCTGGTGAGAAGCATGTCCCTGTGCATTTAGCTGTTTGCATTGCTAAAAATGTTGCTTCTGCACTGGTGGAGCTGCATTCAAAGAATATCATTCATCGAGACGTCAAAAGTGAGAACATTTTGATTGATTTGAATAGCAAGACAGCTGATGGGACCCCTGTTGTGAAGCTCTGTGATTTTGATAGAGCGGTGCCTCTTCAGTCGTACTTGCATTCCTGCCTTGTCGCTCATGTAGGAATACCTCCTCCTAATGTTTGCGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGAGAACAATGCATGAGCAAAATATTTATGGACTGGAAGTTGACATCTGGTCATTTGGATGCCTTCTTTCCGAATTGTTGACTTTGCAACTTCCTTACTCCGGAGTAGCTGATTTGGAGATACATGATCTTATTGTGACAGGACAACGACCTAAGTTAACAGATGAGCTGGAGGCGCTTCTGCTATTTATGGACGAGCCAGTGGCTCGATCAGCTGGAGAGCTTGGGGGAACAGAGGCTGACTTAGACACACTAACATTTCTTGCTGACTTGTTCTGCAAATGTACTGAGGAGAATCCGCAGAGTCGTCCAACAGCTGAGAACATCTATAAATTGTTGCTCGAACACAGCATGCAGCTTACAAACTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1157

Amino Acids

127.96

Weight (kDa)

5.2

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 119 - 260 1.5e-14 Leucine-rich repeat region
LRR_8 PF13855 156 - 212 2.3e-07 Leucine rich repeat
LRR_8 PF13855 202 - 259 3.9e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 697 - 768 1.5e-10 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 870 - 1143 1.2e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 872 - 1088 5e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2036
AasI GACNNNNNNGTC 2 cut(s) 89, 2003
AatII GACGTC 3 cut(s) 132, 710, 2913
Acc36I ACCTGC 1 cut(s) 2036
AccB1I GGYRCC 3 cut(s) 86, 420, 2998
AccB7I CCANNNNNTGG 2 cut(s) 145, 1301
AccBSI CCGCTC 2 cut(s) 2130, 2996
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 1731
AccIII TCCGGA 4 cut(s) 511, 747, 1238, 3200
AciI CCGC 9 cut(s) 365, 428, 1686, 1724, 1809, 2084, 2128, 2996, 3398
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 8 cut(s) 374, 751, 1101, 1468, 1651, 1664, 1768, 1833
AcuI CTGAAG 6 cut(s) 281, 407, 837, 2568, 2691, 2990
AcyI GRCGYC 3 cut(s) 129, 707, 2910
AfeI AGCGCT 1 cut(s) 384
AfiI CCNNNNNNNGG 7 cut(s) 145, 305, 511, 1301, 2292, 2438, 3200
AhdI GACNNNNNGTC 1 cut(s) 3148
AjnI CCWGG 6 cut(s) 144, 423, 681, 967, 2271, 2722
AloI GAACNNNNNNTCC 2 cut(s) 539, 571
Alw21I GWGCWC 2 cut(s) 402, 967
Alw26I GTCTC 4 cut(s) 136, 1972, 2324, 2901
AlwNI CAGNNNCTG 2 cut(s) 2387, 3338
Ama87I CYCGRG 2 cut(s) 1089, 2241
Aor13HI TCCGGA 4 cut(s) 511, 747, 1238, 3200
Aor51HI AGCGCT 1 cut(s) 384
AoxI GGCC 2 cut(s) 136, 1736
ApoI RAATTY 8 cut(s) 374, 751, 1101, 1468, 1651, 1664, 1768, 1833
AseI ATTAAT 1 cut(s) 2055
Asp700I GAANNNNTTC 1 cut(s) 266
AspLEI GCGC 2 cut(s) 385, 3277
AspS9I GGNCC 2 cut(s) 1991, 2961
AsuC2I CCSGG 1 cut(s) 579
AsuHPI GGTGA 9 cut(s) 226, 1151, 1205, 1361, 1415, 1490, 1526, 2057, 2825
AsuII TTCGAA 1 cut(s) 1472
AvaI CYCGRG 2 cut(s) 1089, 2241
AvaII GGWCC 2 cut(s) 1991, 2961
AxyI CCTNAGG 1 cut(s) 648
BamHI GGATCC 2 cut(s) 1241, 1454
BanI GGYRCC 3 cut(s) 86, 420, 2998
BanII GRGCYC 3 cut(s) 402, 967, 2704
BarI GAAGNNNNNNTAC 2 cut(s) 2594, 2626
BauI CACGAG 1 cut(s) 1785
BbsI GAAGAC 4 cut(s) 48, 260, 575, 1694
Bbv12I GWGCWC 2 cut(s) 402, 967
BbvCI CCTCAGC 1 cut(s) 1381
BceAI ACGGC 3 cut(s) 74, 1723, 1848
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 6 cut(s) 146, 425, 683, 969, 2273, 2724
BciVI GTATCC 3 cut(s) 1177, 1387, 2261
BclI TGATCA 1 cut(s) 1624
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 4 cut(s) 136, 1972, 2324, 2901
BfaI CTAG 9 cut(s) 323, 669, 894, 917, 1323, 1340, 2037, 2159, 3468
BfmI CTRYAG 3 cut(s) 1059, 1125, 2166
BfoI RGCGCY 2 cut(s) 386, 3278
BfuAI ACCTGC 1 cut(s) 2036
BfuI GTATCC 3 cut(s) 1177, 1387, 2261
BlpI GCTNAGC 2 cut(s) 740, 871
Bme1390I CCNGG 7 cut(s) 146, 425, 579, 683, 969, 2273, 2724
Bme18I GGWCC 2 cut(s) 1991, 2961
BmeRI GACNNNNNGTC 1 cut(s) 3148
BmeT110I CYCGRG 2 cut(s) 1089, 2241
BmgT120I GGNCC 2 cut(s) 1991, 2961
BmrFI CCNGG 7 cut(s) 146, 425, 579, 683, 969, 2273, 2724
BmsI GCATC 8 cut(s) 550, 726, 962, 981, 1430, 1711, 2338, 3149
BpiI GAAGAC 4 cut(s) 48, 260, 575, 1694
BpmI CTGGAG 6 cut(s) 416, 951, 1772, 3075, 3290, 3336
Bpu10I CCTNAGC 2 cut(s) 1381, 2620
Bpu1102I GCTNAGC 2 cut(s) 740, 871
Bpu14I TTCGAA 1 cut(s) 1472
BpuEI CTTGAG 3 cut(s) 443, 746, 1256
BpuMI CCSGG 1 cut(s) 579
BsaHI GRCGYC 3 cut(s) 129, 707, 2910
BsaJI CCNNGG 2 cut(s) 405, 800
BsaWI WCCGGW 5 cut(s) 236, 511, 747, 1238, 3200
BsaXI ACNNNNNCTCC 4 cut(s) 447, 477, 931, 961
Bsc4I CCNNNNNNNGG 7 cut(s) 145, 305, 511, 1301, 2292, 2438, 3200
Bse1I ACTGG 6 cut(s) 1386, 1755, 2306, 2877, 3138, 3299
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 5 cut(s) 65, 1558, 1917, 2204, 2847
BseAI TCCGGA 4 cut(s) 511, 747, 1238, 3200
BseBI CCWGG 6 cut(s) 146, 425, 683, 969, 2273, 2724
BseDI CCNNGG 2 cut(s) 405, 800
BseLI CCNNNNNNNGG 7 cut(s) 145, 305, 511, 1301, 2292, 2438, 3200
BseMI GCAATG 5 cut(s) 65, 1558, 1917, 2204, 2847
BseMII CTCAG 8 cut(s) 639, 885, 1395, 2106, 2319, 3092, 3378, 3408
BseNI ACTGG 6 cut(s) 1386, 1755, 2306, 2877, 3138, 3299
BseRI GAGGAG 3 cut(s) 455, 3042, 3404
BsgI GTGCAG 3 cut(s) 353, 2198, 2853
Bsh1236I CGCG 1 cut(s) 1731
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 138, 1738
BshNI GGYRCC 3 cut(s) 86, 420, 2998
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 2 cut(s) 402, 967
BsiHKCI CYCGRG 2 cut(s) 1089, 2241
BslFI GGGAC 4 cut(s) 1740, 2593, 2810, 2974
BslI CCNNNNNNNGG 7 cut(s) 145, 305, 511, 1301, 2292, 2438, 3200
BsmAI GTCTC 4 cut(s) 136, 1972, 2324, 2901
BsmBI CGTCTC 3 cut(s) 136, 1972, 2901
BsmFI GGGAC 4 cut(s) 1740, 2593, 2810, 2974
BsmI GAATGC 4 cut(s) 332, 604, 2884, 3019
BsnI GGCC 2 cut(s) 138, 1738
BsoBI CYCGRG 2 cut(s) 1089, 2241
Bsp119I TTCGAA 1 cut(s) 1472
Bsp1286I GDGCHC 3 cut(s) 402, 967, 2704
Bsp13I TCCGGA 4 cut(s) 511, 747, 1238, 3200
Bsp1407I TGTACA 1 cut(s) 210
Bsp1720I GCTNAGC 2 cut(s) 740, 871
BspACI CCGC 9 cut(s) 365, 428, 1686, 1724, 1809, 2084, 2128, 2996, 3398
BspANI GGCC 2 cut(s) 138, 1738
BspCNI CTCAG 8 cut(s) 640, 884, 1394, 2107, 2320, 3093, 3379, 3409
BspEI TCCGGA 4 cut(s) 511, 747, 1238, 3200
BspFNI CGCG 1 cut(s) 1731
BspHI TCATGA 1 cut(s) 1639
BspMAI CTGCAG 2 cut(s) 1063, 1129
BspMI ACCTGC 1 cut(s) 2036
BspT104I TTCGAA 1 cut(s) 1472
BspT107I GGYRCC 3 cut(s) 86, 420, 2998
BsrBI CCGCTC 2 cut(s) 2130, 2996
BsrDI GCAATG 5 cut(s) 65, 1558, 1917, 2204, 2847
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 6 cut(s) 1386, 1755, 2306, 2877, 3138, 3299
BssECI CCNNGG 2 cut(s) 405, 800
BssNI GRCGYC 3 cut(s) 129, 707, 2910
BssSI CACGAG 1 cut(s) 1785
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 1785
Bst2UI CCWGG 6 cut(s) 146, 425, 683, 969, 2273, 2724
Bst4CI ACNGT 6 cut(s) 86, 359, 1178, 1700, 2045, 2176
Bst6I CTCTTC 1 cut(s) 3009
BstACI GRCGYC 3 cut(s) 129, 707, 2910
BstAUI TGTACA 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 1472
BstC8I GCNNGC 2 cut(s) 2082, 3452
BstDSI CCRYGG 1 cut(s) 800
BstFNI CGCG 1 cut(s) 1731
BstH2I RGCGCY 2 cut(s) 386, 3278
BstHHI GCGC 2 cut(s) 385, 3277
BstMAI GTCTC 4 cut(s) 136, 1972, 2324, 2901
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 8 cut(s) 391, 700, 1492, 1548, 1744, 2724, 3274, 3311
BstNI CCWGG 6 cut(s) 146, 425, 683, 969, 2273, 2724
BstNSI RCATGY 5 cut(s) 1262, 1490, 2355, 2825, 3454
BstSCI CCNGG 7 cut(s) 144, 423, 577, 681, 967, 2271, 2722
BstSFI CTRYAG 3 cut(s) 1059, 1125, 2166
BstUI CGCG 1 cut(s) 1731
BstV2I GAAGAC 4 cut(s) 48, 260, 575, 1694
BstX2I RGATCY 7 cut(s) 187, 336, 547, 1241, 1267, 1454, 2383
BstXI CCANNNNNNTGG 1 cut(s) 1855
BstYI RGATCY 7 cut(s) 187, 336, 547, 1241, 1267, 1454, 2383
Bsu36I CCTNAGG 1 cut(s) 648
BsuI GTATCC 3 cut(s) 1177, 1387, 2261
BsuRI GGCC 2 cut(s) 138, 1738
BtgI CCRYGG 1 cut(s) 800
BtsIMutI CAGTG 6 cut(s) 1748, 1890, 2528, 2715, 2870, 3306
BveI ACCTGC 1 cut(s) 2036
Cac8I GCNNGC 2 cut(s) 2082, 3452
CaiI CAGNNNCTG 2 cut(s) 2387, 3338
CciI TCATGA 1 cut(s) 1639
CfoI GCGC 2 cut(s) 385, 3277
Cfr13I GGNCC 2 cut(s) 1991, 2961
CseI GACGC 3 cut(s) 265, 1737, 1989
CspCI CAANNNNNGTGG 2 cut(s) 2454, 2489
DrdI GACNNNNNNGTC 2 cut(s) 89, 2003
DriI GACNNNNNGTC 1 cut(s) 3148
DseDI GACNNNNNNGTC 2 cut(s) 89, 2003
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 3009
Eam1105I GACNNNNNGTC 1 cut(s) 3148
EarI CTCTTC 1 cut(s) 3009
Ecl136II GAGCTC 2 cut(s) 400, 965
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 3 cut(s) 402, 967, 2704
Eco32I GATATC 2 cut(s) 2110, 2688
Eco47I GGWCC 2 cut(s) 1991, 2961
Eco47III AGCGCT 1 cut(s) 384
Eco53kI GAGCTC 2 cut(s) 400, 965
Eco57I CTGAAG 6 cut(s) 281, 407, 837, 2568, 2691, 2990
Eco81I CCTNAGG 1 cut(s) 648
Eco88I CYCGRG 2 cut(s) 1089, 2241
EcoICRI GAGCTC 2 cut(s) 400, 965
EcoO109I RGGNCCY 1 cut(s) 2961
EcoRI GAATTC 1 cut(s) 1468
EcoRII CCWGG 6 cut(s) 144, 423, 681, 967, 2271, 2722
EcoRV GATATC 2 cut(s) 2110, 2688
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 3 cut(s) 332, 2353, 3114
EcoT38I GRGCYC 3 cut(s) 402, 967, 2704
ErhI CCWWGG 1 cut(s) 405
Esp3I CGTCTC 3 cut(s) 136, 1972, 2901
FalI AAGNNNNNCTT 2 cut(s) 2418, 2450
FaqI GGGAC 4 cut(s) 1740, 2593, 2810, 2974
FauI CCCGC 1 cut(s) 1717
FbaI TGATCA 1 cut(s) 1624
FblI GTMKAC 1 cut(s) 78
FriOI GRGCYC 3 cut(s) 402, 967, 2704
FspBI CTAG 9 cut(s) 323, 669, 894, 917, 1323, 1340, 2037, 2159, 3468
GlaI GCGC 2 cut(s) 384, 3276
GsuI CTGGAG 6 cut(s) 416, 951, 1772, 3075, 3290, 3336
HaeII RGCGCY 2 cut(s) 386, 3278
HaeIII GGCC 2 cut(s) 138, 1738
HgaI GACGC 3 cut(s) 265, 1737, 1989
HhaI GCGC 2 cut(s) 385, 3277
Hin1I GRCGYC 3 cut(s) 129, 707, 2910
Hin6I GCGC 2 cut(s) 383, 3275
HinP1I GCGC 2 cut(s) 383, 3275
HincII GTYRAC 6 cut(s) 79, 1117, 2320, 3142, 3180, 3258
HindII GTYRAC 6 cut(s) 79, 1117, 2320, 3142, 3180, 3258
HpaI GTTAAC 2 cut(s) 1117, 3258
HphI GGTGA 9 cut(s) 226, 1151, 1205, 1361, 1415, 1490, 1526, 2057, 2825
Hpy166II GTNNAC 8 cut(s) 79, 211, 1117, 2048, 2320, 3142, 3180, 3258
Hpy8I GTNNAC 8 cut(s) 79, 211, 1117, 2048, 2320, 3142, 3180, 3258
HpyAV CCTTC 8 cut(s) 382, 515, 1006, 1070, 1130, 1246, 2715, 3173
HpyCH4III ACNGT 6 cut(s) 86, 359, 1178, 1700, 2045, 2176
HpyCH4IV ACGT 5 cut(s) 129, 707, 1618, 1676, 2910
HpyF10VI GCNNNNNNNGC 8 cut(s) 391, 700, 1492, 1548, 1744, 2724, 3274, 3311
HpySE526I ACGT 5 cut(s) 129, 707, 1618, 1676, 2910
Hsp92I GRCGYC 3 cut(s) 129, 707, 2910
HspAI GCGC 2 cut(s) 383, 3275
KflI GGGWCCC 1 cut(s) 2961
Kpn2I TCCGGA 4 cut(s) 511, 747, 1238, 3200
Ksp22I TGATCA 1 cut(s) 1624
KspAI GTTAAC 2 cut(s) 1117, 3258
LweI GCATC 8 cut(s) 550, 726, 962, 981, 1430, 1711, 2338, 3149
MaeI CTAG 9 cut(s) 323, 669, 894, 917, 1323, 1340, 2037, 2159, 3468
MaeII ACGT 5 cut(s) 129, 707, 1618, 1676, 2910
MaeIII GTNAC 4 cut(s) 409, 1048, 2786, 3235
MbiI CCGCTC 2 cut(s) 2130, 2996
MflI RGATCY 7 cut(s) 187, 336, 547, 1241, 1267, 1454, 2383
MhlI GDGCHC 3 cut(s) 402, 967, 2704
MlyI GAGTC 7 cut(s) 110, 450, 848, 1775, 2315, 2326, 3412
MmeI TCCRAC 8 cut(s) 654, 843, 905, 912, 1806, 2318, 3049, 3434
Mph1103I ATGCAT 3 cut(s) 332, 2353, 3114
MroI TCCGGA 4 cut(s) 511, 747, 1238, 3200
MroXI GAANNNNTTC 1 cut(s) 266
MslI CAYNNNNRTG 5 cut(s) 418, 1001, 1638, 2695, 3157
MspA1I CMGCKG 5 cut(s) 367, 1809, 2954, 3314, 3416
MspR9I CCNGG 7 cut(s) 146, 425, 579, 683, 969, 2273, 2724
Mva1269I GAATGC 4 cut(s) 332, 604, 2884, 3019
MvaI CCWGG 6 cut(s) 146, 425, 683, 969, 2273, 2724
MvnI CGCG 1 cut(s) 1731
MwoI GCNNNNNNNGC 8 cut(s) 391, 700, 1492, 1548, 1744, 2724, 3274, 3311
NciI CCSGG 1 cut(s) 579
NmuCI GTSAC 2 cut(s) 409, 3235
NsiI ATGCAT 3 cut(s) 332, 2353, 3114
NspI RCATGY 5 cut(s) 1262, 1490, 2355, 2825, 3454
NspV TTCGAA 1 cut(s) 1472
PaeI GCATGC 1 cut(s) 3454
PaeR7I CTCGAG 2 cut(s) 1089, 2241
PagI TCATGA 1 cut(s) 1639
PaqCI CACCTGC 1 cut(s) 2036
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 90
PctI GAATGC 4 cut(s) 332, 604, 2884, 3019
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 5 cut(s) 59, 757, 1055, 1291, 3394
PflFI GACNNNGTC 1 cut(s) 179
PflMI CCANNNNNTGG 2 cut(s) 145, 1301
PfoI TCCNGGA 1 cut(s) 967
Ple19I CGATCG 1 cut(s) 159
PleI GAGTC 7 cut(s) 110, 450, 847, 1775, 2315, 2326, 3411
PpsI GAGTC 7 cut(s) 110, 450, 847, 1775, 2315, 2326, 3411
PpuMI RGGWCCY 1 cut(s) 2961
PshBI ATTAAT 1 cut(s) 2055
Psp124BI GAGCTC 2 cut(s) 402, 967
Psp5II RGGWCCY 1 cut(s) 2961
Psp6I CCWGG 6 cut(s) 144, 423, 681, 967, 2271, 2722
PspGI CCWGG 6 cut(s) 144, 423, 681, 967, 2271, 2722
PspPI GGNCC 2 cut(s) 1991, 2961
PspPPI RGGWCCY 1 cut(s) 2961
PspXI VCTCGAGB 1 cut(s) 2241
PstI CTGCAG 2 cut(s) 1063, 1129
PstNI CAGNNNCTG 2 cut(s) 2387, 3338
PsuI RGATCY 7 cut(s) 187, 336, 547, 1241, 1267, 1454, 2383
PsyI GACNNNGTC 1 cut(s) 179
PvuI CGATCG 1 cut(s) 159
PvuII CAGCTG 3 cut(s) 2954, 3314, 3416
RseI CAYNNNNRTG 5 cut(s) 418, 1001, 1638, 2695, 3157
SacI GAGCTC 2 cut(s) 402, 967
SalI GTCGAC 1 cut(s) 77
Sau96I GGNCC 2 cut(s) 1991, 2961
SchI GAGTC 7 cut(s) 110, 450, 848, 1775, 2315, 2326, 3412
ScrFI CCNGG 7 cut(s) 146, 425, 579, 683, 969, 2273, 2724
SduI GDGCHC 3 cut(s) 402, 967, 2704
SfaNI GCATC 8 cut(s) 550, 726, 962, 981, 1430, 1711, 2338, 3149
SfcI CTRYAG 3 cut(s) 1059, 1125, 2166
Sfr274I CTCGAG 2 cut(s) 1089, 2241
SfuI TTCGAA 1 cut(s) 1472
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 2 cut(s) 1991, 2961
SlaI CTCGAG 2 cut(s) 1089, 2241
SmiMI CAYNNNNRTG 5 cut(s) 418, 1001, 1638, 2695, 3157
SmlI CTYRAG 5 cut(s) 458, 761, 1089, 1271, 2241
SmoI CTYRAG 5 cut(s) 458, 761, 1089, 1271, 2241
SphI GCATGC 1 cut(s) 3454
SsiI CCGC 9 cut(s) 365, 428, 1686, 1724, 1809, 2084, 2128, 2996, 3398
SspI AATATT 3 cut(s) 2299, 2393, 3124
SspMI CTAG 9 cut(s) 323, 669, 894, 917, 1323, 1340, 2037, 2159, 3468
SstI GAGCTC 2 cut(s) 402, 967
StyD4I CCNGG 7 cut(s) 144, 423, 577, 681, 967, 2271, 2722
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 6 cut(s) 86, 359, 1178, 1700, 2045, 2176
TaiI ACGT 5 cut(s) 132, 710, 1621, 1679, 2913
TatI WGTACW 6 cut(s) 210, 765, 1630, 2610, 2760, 3383
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 5 cut(s) 59, 757, 1055, 1291, 3394
TscAI CASTG 6 cut(s) 1755, 1897, 2528, 2715, 2877, 3306
TseFI GTSAC 2 cut(s) 409, 3235
Tsp45I GTSAC 2 cut(s) 409, 3235
TspGWI ACGGA 4 cut(s) 7, 789, 1990, 2434
TspRI CASTG 6 cut(s) 1755, 1897, 2528, 2715, 2877, 3306
Tth111I GACNNNGTC 1 cut(s) 179
Van91I CCANNNNNTGG 2 cut(s) 145, 1301
VpaK11BI GGWCC 2 cut(s) 1991, 2961
VspI ATTAAT 1 cut(s) 2055
XapI RAATTY 8 cut(s) 374, 751, 1101, 1468, 1651, 1664, 1768, 1833
XceI RCATGY 5 cut(s) 1262, 1490, 2355, 2825, 3454
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XhoI CTCGAG 2 cut(s) 1089, 2241
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 9 cut(s) 323, 669, 894, 917, 1323, 1340, 2037, 2159, 3468
ZraI GACGTC 3 cut(s) 130, 708, 2911
Zsp2I ATGCAT 3 cut(s) 332, 2353, 3114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.