FvH4_4g31210

Cyclic dof factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003750
Physical Location & Seq
Reverse (-)
30455450 .. 30458948
3499 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31210.t1

Sequence Viewer

Length: 1578 bp
ATGTCTGAGAAAGAAATCAGCCCCACTATAAAGCTCTTTGGACAGACCATACCTTTGGCTTCGGCGCCACTCAGCGGCCATGAGATTCCGACCAATGATGACCCGCCATGTGGTGCTTCCGCCTCTGCTGCTGACTCTAAGATTGCAGCTGCTGCTGCTGGTGTTGTTGAAGAAGAAGACTCCTCAGTCAAGCTTCCTTCTTGTTCAGAAACTACTTCACCTGAAGAGCCAGAGAACAAGGAAACAATATCAGGAAAAGAACTCGCTGCTGACACAGAGGAAGAAAATCCCTCAGATCAATTCACAGAAGACTTGAAACCTCCGACAACATCATCGGGAATTTGTGAGAATCCTAAAACTCCCTCAGCTGATAGAGAAACTTCAGTATCTCTGAAATCCTCTAAGAATGGAGAACAGAGTGATGAGACCAGTAGCTCACAAGACAAGACTCTGAAGAAGCCAGACAAGATACTTCCATGTCCCCGATGTAATAGCATGGACACCAAGTTTTGTTACTATAACAATTACAATGTCAATCAGCCGCGCCATTTTTGCAAGAACTGTCAAAGATACTGGACTGCCGGAGGAATCATGAGGAATGTGCCTGTGGGAGCTGGTCGTCGCAAGAACAAAAATGCTTCGTCGTCAGCTTCACACTATCGTCACATGTTAATGTCGGATGCTCTCCAAACTGCTCATTCTAGTGCTGCTGCTGCTGCTTCTAATGGTGCGCACAATCCAACTTTGGGAAGAAATGGCACTGTCCTTACCTTTGGATCAGATTCTCCTCTTTGTGAATCAATGGCTTCTGTATTGAGTCTCGCCGAGAAAACAAAAAACTCTGCTCAGAACGGGTTTCATGTACCTGATCAACAAAGAATTCTGGTTCCTTCGATAGCTGGAGATAATGGGGATGACCAGTCAAGTGGATCTTCTAATGTTACAGCTTCATATTCATCAGAGAGGGTATGCAAATCTGGTAAACAAGAACCAGCGGTTCACAACTGTCAGGGTTTCCCTTCTCATCAAGTACCATGCTTTCCGGGTGCTCCTTGGCCTTATGCATGGAACTCACCTCAGTGGACCTCTGCAATGCCTCCACCTGCATTCTTCCATTCAGGGTTTCCCATATCATTCTACCCTGCACCACCGTACTGGGGCTGCACCGGACCCGGCTCTTGGAATGTACCATGTAACTCTCCACCATCCTCCACTATTAGCCATTGTGGTACAAGCTCCGGTCCAAATTCCCCAACCTTAGGGAAACATCCTAGAGATGGGGACATTCTTCATCAAGCCAGCTCTCAAAAAGAAGAGTCCAGCAGACAGAACAAGTCAGGATGTGTTTTGATTCCTAAAACGTTGAGGATTGACGATCCTAGTGAAGCTGCAAAGAGCTCTATATGGGAAACACTTGGCATCAAGAATGAAAAGGGAAATTCTATTAACGAGGGAGGTATCTTCAAGTCATTTCAATCCAAGGCAGATGAGAGAAGGCAAGTTGTTGAACAATCGTCGGTGTTGCAAGCTAATCCTGCAGCCTTTTCACGGTCACTCAACTTCCATGAGAGATCATGA

Protein Analysis

526

Amino Acids

56.17

Weight (kDa)

6.08

Isoelectric Point (pI)

55.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 156 - 212 1.4e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1111
AasI GACNNNNNNGTC 1 cut(s) 185
Acc16I TGCGCA 1 cut(s) 732
Acc36I ACCTGC 1 cut(s) 1111
AccB1I GGYRCC 1 cut(s) 64
AccII CGCG 1 cut(s) 544
AciI CCGC 5 cut(s) 75, 104, 120, 542, 995
AclI AACGTT 1 cut(s) 1361
AclWI GGATC 3 cut(s) 784, 937, 1370
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 4 cut(s) 339, 879, 1246, 1438
AcuI CTGAAG 3 cut(s) 243, 366, 473
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 5 cut(s) 864, 1032, 1154, 1188, 1231
AfiI CCNNNNNNNGG 8 cut(s) 74, 110, 746, 1157, 1179, 1259, 1277, 1548
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 5 cut(s) 170, 316, 1465, 1475, 1508
AleI CACNNNNGTG 1 cut(s) 1078
Alw21I GWGCWC 2 cut(s) 1051, 1400
Alw26I GTCTC 2 cut(s) 419, 824
AlwI GGATC 3 cut(s) 784, 937, 1370
AlwNI CAGNNNCTG 1 cut(s) 152
AoxI GGCC 2 cut(s) 76, 1055
ApoI RAATTY 4 cut(s) 339, 879, 1246, 1438
ArsI GACNNNNNNTTYG 2 cut(s) 37, 69
AspLEI GCGC 3 cut(s) 67, 546, 733
AspS9I GGNCC 3 cut(s) 1083, 1169, 1241
AsuC2I CCSGG 2 cut(s) 1044, 1173
AsuHPI GGTGA 2 cut(s) 210, 1065
AvaII GGWCC 3 cut(s) 1083, 1169, 1241
AxyI CCTNAGG 1 cut(s) 1258
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BanI GGYRCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 1400
BbsI GAAGAC 2 cut(s) 183, 315
Bbv12I GWGCWC 2 cut(s) 1051, 1400
BbvCI CCTCAGC 1 cut(s) 364
BccI CCATC 2 cut(s) 1213, 1271
BclI TGATCA 1 cut(s) 868
BcnI CCSGG 2 cut(s) 1044, 1173
BcoDI GTCTC 2 cut(s) 419, 824
BfaI CTAG 3 cut(s) 702, 1272, 1380
BfmI CTRYAG 1 cut(s) 1536
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 1111
Bme1390I CCNGG 2 cut(s) 1044, 1173
Bme18I GGWCC 3 cut(s) 1083, 1169, 1241
BmgT120I GGNCC 3 cut(s) 1083, 1169, 1241
BmiI GGNNCC 3 cut(s) 66, 888, 1171
BmrFI CCNGG 2 cut(s) 1044, 1173
BmrI ACTGGG 1 cut(s) 1165
BmsI GCATC 2 cut(s) 670, 1428
BmuI ACTGGG 1 cut(s) 1165
BpiI GAAGAC 2 cut(s) 183, 315
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 1 cut(s) 364
BpuMI CCSGG 2 cut(s) 1044, 1173
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 2 cut(s) 1052, 1479
BsaWI WCCGGW 2 cut(s) 1166, 1238
Bsc4I CCNNNNNNNGG 8 cut(s) 74, 110, 746, 1157, 1179, 1259, 1277, 1548
Bse1I ACTGG 4 cut(s) 429, 578, 919, 1160
Bse21I CCTNAGG 1 cut(s) 1258
Bse3DI GCAATG 1 cut(s) 1098
BseDI CCNNGG 2 cut(s) 1052, 1479
BseGI GGATG 5 cut(s) 685, 919, 1205, 1267, 1346
BseLI CCNNNNNNNGG 8 cut(s) 74, 110, 746, 1157, 1179, 1259, 1277, 1548
BseMI GCAATG 1 cut(s) 1098
BseMII CTCAG 6 cut(s) 85, 198, 306, 378, 860, 1091
BseNI ACTGG 4 cut(s) 429, 578, 919, 1160
BseRI GAGGAG 2 cut(s) 172, 777
BsgI GTGCAG 2 cut(s) 1128, 1147
Bsh1236I CGCG 1 cut(s) 544
BshFI GGCC 2 cut(s) 78, 1057
BshNI GGYRCC 1 cut(s) 64
BsiHKAI GWGCWC 2 cut(s) 1051, 1400
BsiSI CCGG 5 cut(s) 582, 1043, 1167, 1173, 1239
BslFI GGGAC 2 cut(s) 465, 1295
BslI CCNNNNNNNGG 8 cut(s) 74, 110, 746, 1157, 1179, 1259, 1277, 1548
BsmAI GTCTC 2 cut(s) 419, 824
BsmFI GGGAC 2 cut(s) 465, 1295
BsmI GAATGC 1 cut(s) 1106
BsnI GGCC 2 cut(s) 78, 1057
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 2 cut(s) 1051, 1400
Bsp143I GATC 6 cut(s) 295, 776, 868, 929, 1375, 1571
BspACI CCGC 5 cut(s) 75, 104, 120, 542, 995
BspANI GGCC 2 cut(s) 78, 1057
BspCNI CTCAG 6 cut(s) 84, 197, 305, 377, 859, 1090
BspFNI CGCG 1 cut(s) 544
BspHI TCATGA 2 cut(s) 591, 1574
BspLI GGNNCC 3 cut(s) 66, 888, 1171
BspMAI CTGCAG 1 cut(s) 1540
BspMI ACCTGC 1 cut(s) 1111
BspPI GGATC 3 cut(s) 784, 937, 1370
BspQI GCTCTTC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 1098
BsrI ACTGG 4 cut(s) 429, 578, 919, 1160
BssECI CCNNGG 2 cut(s) 1052, 1479
BssMI GATC 6 cut(s) 295, 776, 868, 929, 1375, 1571
BssNI GRCGYC 1 cut(s) 65
BssT1I CCWWGG 2 cut(s) 1052, 1479
Bst4CI ACNGT 5 cut(s) 563, 763, 1007, 1152, 1551
Bst6I CTCTTC 2 cut(s) 219, 1308
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 1300, 1527
BstF5I GGATG 5 cut(s) 685, 919, 1205, 1267, 1346
BstFNI CGCG 1 cut(s) 544
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 3 cut(s) 67, 546, 733
BstKTI GATC 6 cut(s) 298, 779, 871, 932, 1378, 1574
BstMAI GTCTC 2 cut(s) 419, 824
BstMBI GATC 6 cut(s) 295, 776, 868, 929, 1375, 1571
BstMWI GCNNNNNNNGC 7 cut(s) 128, 152, 155, 552, 713, 716, 1535
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 2 cut(s) 1042, 1171
BstSFI CTRYAG 1 cut(s) 1536
BstUI CGCG 1 cut(s) 544
BstV2I GAAGAC 2 cut(s) 183, 315
BstX2I RGATCY 1 cut(s) 929
BstXI CCANNNNNNTGG 3 cut(s) 55, 926, 1155
BstYI RGATCY 1 cut(s) 929
Bsu36I CCTNAGG 1 cut(s) 1258
BsuRI GGCC 2 cut(s) 78, 1057
BtsCI GGATG 5 cut(s) 685, 919, 1205, 1267, 1346
BtsIMutI CAGTG 2 cut(s) 759, 1085
BveI ACCTGC 1 cut(s) 1111
Cac8I GCNNGC 2 cut(s) 1300, 1527
CaiI CAGNNNCTG 1 cut(s) 152
CciI TCATGA 2 cut(s) 591, 1574
CfoI GCGC 3 cut(s) 67, 546, 733
Cfr13I GGNCC 3 cut(s) 1083, 1169, 1241
Csp6I GTAC 5 cut(s) 863, 1031, 1153, 1187, 1230
CviQI GTAC 5 cut(s) 863, 1031, 1153, 1187, 1230
DinI GGCGCC 1 cut(s) 66
DpnI GATC 6 cut(s) 297, 778, 870, 931, 1377, 1573
DpnII GATC 6 cut(s) 295, 776, 868, 929, 1375, 1571
DrdI GACNNNNNNGTC 1 cut(s) 185
DseDI GACNNNNNNGTC 1 cut(s) 185
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 2 cut(s) 219, 1308
EarI CTCTTC 2 cut(s) 219, 1308
EciI GGCGGA 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 1398
Eco130I CCWWGG 2 cut(s) 1052, 1479
Eco24I GRGCYC 1 cut(s) 1400
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 3 cut(s) 1083, 1169, 1241
Eco53kI GAGCTC 1 cut(s) 1398
Eco57I CTGAAG 3 cut(s) 243, 366, 473
Eco81I CCTNAGG 1 cut(s) 1258
EcoICRI GAGCTC 1 cut(s) 1398
EcoRI GAATTC 1 cut(s) 879
EcoT14I CCWWGG 2 cut(s) 1052, 1479
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 1 cut(s) 1400
EgeI GGCGCC 1 cut(s) 66
EheI GGCGCC 1 cut(s) 66
ErhI CCWWGG 2 cut(s) 1052, 1479
FalI AAGNNNNNCTT 2 cut(s) 916, 948
FaqI GGGAC 2 cut(s) 465, 1295
FauI CCCGC 1 cut(s) 111
FbaI TGATCA 1 cut(s) 868
FokI GGATG 5 cut(s) 692, 926, 1192, 1254, 1353
FriOI GRGCYC 1 cut(s) 1400
FspAI RTGCGCAY 1 cut(s) 732
FspBI CTAG 3 cut(s) 702, 1272, 1380
FspI TGCGCA 1 cut(s) 732
GlaI GCGC 3 cut(s) 66, 545, 732
GsuI CTGGAG 1 cut(s) 921
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 2 cut(s) 78, 1057
HapII CCGG 5 cut(s) 582, 1043, 1167, 1173, 1239
HhaI GCGC 3 cut(s) 67, 546, 733
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 3 cut(s) 65, 544, 731
HinP1I GCGC 3 cut(s) 65, 544, 731
HindIII AAGCTT 1 cut(s) 191
HpaII CCGG 5 cut(s) 582, 1043, 1167, 1173, 1239
HphI GGTGA 2 cut(s) 210, 1065
Hpy166II GTNNAC 3 cut(s) 983, 1000, 1083
Hpy188III TCNNGA 6 cut(s) 252, 336, 592, 1338, 1423, 1575
Hpy8I GTNNAC 3 cut(s) 983, 1000, 1083
Hpy99I CGWCG 3 cut(s) 624, 646, 1519
HpyAV CCTTC 4 cut(s) 207, 900, 1029, 1488
HpyCH4III ACNGT 5 cut(s) 563, 763, 1007, 1152, 1551
HpyCH4IV ACGT 1 cut(s) 1361
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 152, 155, 552, 713, 716, 1535
HpySE526I ACGT 1 cut(s) 1361
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 3 cut(s) 65, 544, 731
KasI GGCGCC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 868
Kzo9I GATC 6 cut(s) 295, 776, 868, 929, 1375, 1571
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 3 cut(s) 611, 1054, 1241
LweI GCATC 2 cut(s) 670, 1428
MaeI CTAG 3 cut(s) 702, 1272, 1380
MaeII ACGT 1 cut(s) 1361
MaeIII GTNAC 5 cut(s) 512, 662, 940, 1193, 1551
MalI GATC 6 cut(s) 297, 778, 870, 931, 1377, 1573
MboI GATC 6 cut(s) 295, 776, 868, 929, 1375, 1571
MflI RGATCY 1 cut(s) 929
MhlI GDGCHC 2 cut(s) 1051, 1400
MluCI AATT 6 cut(s) 299, 339, 523, 879, 1246, 1438
Mly113I GGCGCC 1 cut(s) 65
MlyI GAGTC 5 cut(s) 128, 173, 442, 826, 1325
MmeI TCCRAC 4 cut(s) 113, 347, 657, 764
Mph1103I ATGCAT 1 cut(s) 1066
MseI TTAA 2 cut(s) 671, 1446
MslI CAYNNNNRTG 3 cut(s) 671, 702, 1078
MspA1I CMGCKG 4 cut(s) 75, 149, 368, 995
MspI CCGG 5 cut(s) 582, 1043, 1167, 1173, 1239
MspR9I CCNGG 2 cut(s) 1044, 1173
Mva1269I GAATGC 1 cut(s) 1106
MvnI CGCG 1 cut(s) 544
MwoI GCNNNNNNNGC 7 cut(s) 128, 152, 155, 552, 713, 716, 1535
NarI GGCGCC 1 cut(s) 65
NciI CCSGG 2 cut(s) 1044, 1173
NdeII GATC 6 cut(s) 295, 776, 868, 929, 1375, 1571
NlaIV GGNNCC 3 cut(s) 66, 888, 1171
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 2 cut(s) 662, 1551
NsbI TGCGCA 1 cut(s) 732
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 1 cut(s) 670
OliI CACNNNNGTG 1 cut(s) 1078
PagI TCATGA 2 cut(s) 591, 1574
PaqCI CACCTGC 1 cut(s) 1111
PciI ACATGT 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 219
PctI GAATGC 1 cut(s) 1106
PfeI GAWTC 6 cut(s) 85, 349, 588, 782, 797, 1351
PleI GAGTC 5 cut(s) 128, 173, 442, 825, 1324
PluTI GGCGCC 1 cut(s) 68
PpsI GAGTC 5 cut(s) 128, 173, 442, 825, 1324
PscI ACATGT 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 1400
Psp1406I AACGTT 1 cut(s) 1361
PspN4I GGNNCC 3 cut(s) 66, 888, 1171
PspPI GGNCC 3 cut(s) 1083, 1169, 1241
PstI CTGCAG 1 cut(s) 1540
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 929
PvuII CAGCTG 2 cut(s) 149, 368
RsaI GTAC 5 cut(s) 864, 1032, 1154, 1188, 1231
RsaNI GTAC 5 cut(s) 863, 1031, 1153, 1187, 1230
RseI CAYNNNNRTG 3 cut(s) 671, 702, 1078
SacI GAGCTC 1 cut(s) 1400
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 671, 1446
Sau3AI GATC 6 cut(s) 295, 776, 868, 929, 1375, 1571
Sau96I GGNCC 3 cut(s) 1083, 1169, 1241
SchI GAGTC 5 cut(s) 128, 173, 442, 826, 1325
ScrFI CCNGG 2 cut(s) 1044, 1173
SduI GDGCHC 2 cut(s) 1051, 1400
SfaNI GCATC 2 cut(s) 670, 1428
SfcI CTRYAG 1 cut(s) 1536
SfoI GGCGCC 1 cut(s) 66
SinI GGWCC 3 cut(s) 1083, 1169, 1241
SmiMI CAYNNNNRTG 3 cut(s) 671, 702, 1078
Sse9I AATT 6 cut(s) 299, 339, 523, 879, 1246, 1438
SsiI CCGC 5 cut(s) 75, 104, 120, 542, 995
SspDI GGCGCC 1 cut(s) 64
SspMI CTAG 3 cut(s) 702, 1272, 1380
SstI GAGCTC 1 cut(s) 1400
StyD4I CCNGG 2 cut(s) 1042, 1171
StyI CCWWGG 2 cut(s) 1052, 1479
TaaI ACNGT 5 cut(s) 563, 763, 1007, 1152, 1551
TaiI ACGT 1 cut(s) 1364
TaqI TCGA 1 cut(s) 893
TasI AATT 6 cut(s) 299, 339, 523, 879, 1246, 1438
TauI GCSGC 2 cut(s) 78, 544
TfiI GAWTC 6 cut(s) 85, 349, 588, 782, 797, 1351
Tru1I TTAA 2 cut(s) 671, 1446
Tru9I TTAA 2 cut(s) 671, 1446
TscAI CASTG 2 cut(s) 766, 1085
TseFI GTSAC 2 cut(s) 662, 1551
Tsp45I GTSAC 2 cut(s) 662, 1551
TspDTI ATGAA 5 cut(s) 848, 939, 945, 1280, 1443
TspRI CASTG 2 cut(s) 766, 1085
VpaK11BI GGWCC 3 cut(s) 1083, 1169, 1241
XapI RAATTY 4 cut(s) 339, 879, 1246, 1438
XceI RCATGY 1 cut(s) 670
XspI CTAG 3 cut(s) 702, 1272, 1380
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.