Rroxscaffold_5G00380530

Cyclic dof factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003750
Physical Location & Seq
Reverse (-)
60662852 .. 60665904
3053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380530.1

Sequence Viewer

Length: 1383 bp
ATGTCTGAGACTGAAATCAGCCCCACCATAAAGCTCTTCGGAAAGACCATACCCTTGGCTTCGGCGCCACACAACGGCCATGAGGTTCCGACCAATGATGACCCGCCATGTGGAGCTTCCGACTGTGCTGTTGACTCTAAGATTTCTGCTGCTGCTGCTGTTGAAGAAGAAGATTGCTCAGTCAAGCTTCCTTGTTGTTCAGAAACTACTTCGCCTGAAGACCAAGAGAACAAGGAAACAATATCAGGCAAAGAACTCACTGCTGACAAAGAGGAAGATAATTCCTTAGATCAATCCACAGAAGACTTGAAACCTCCAACAACATCGTCGGGAATTAGTGAGAATCCCAAAACTCCCTCAGCTGATAGAGAAACTTCAGTATCTCTGAAATCTTCTAAGAATGGAGAACAGAGTGATGAGACCAGTTGCTCACAAGACAAGACTCTGAAGAAGCCAGACAAGATACTTCCATGTCCCCGATGTAATAGCATGGACACCAAGTTCTGTTACTACAACAACTACAATGTCAATCAGCCCCGCCATTTTTGCAAGAACTGTCAAAGATACTGGACTGCCGGAGGAATCATGAGGAATGTTCCTGTGGGAGCTGGTCGTCGCAAGAACAAGAACGCTTCATCAGCTTCACACTATCGTCACATGTTAATGTCGGATGCTCTCCAAACAGCTCATGCTAGTGCTACTGCTGCTTCTAATGGTGCGCACAATCCAACTTTGGGAAGAAATGGCACTGTCCTTACCTTTGGTTCAGATTCTCCTCTTTGTGAATCAATGGCTTCTGTATTGAACCTCGCCGAGAAAACACAAAACTCTGTTCAGAATGGGTTTCATGTACCTGATCAACAAAGAATTCTGGTTCCTTCCATAACTGGAGATAATGGGGATGACCAATCAAGTGGATCGTCTAATGTTACAGCTTCGAATTCATCAGAGGGGGATGCAAAGCTGGTATACAAGAACCAGCAGTTCACAACTGTCAAAGCTTCTCTCCTCATCAAGTACCATGCTTTCCAGGCTCCCCTTGGCCTTATCCATGGAACTCACCTCAGTGGACCTCTGCAATGCCTCCACCTGCATTCTTCCATTCAGGGTTTCCCATATCATTCTACCCTGCATTACCATACTGGGGCTGCACTGGACCTGGCTCTTGGAATGATTGATGATCCTAGTGAAGCTGCAAAGAGCTCTATATGGGAAACACTTGGCATCAAGAATGAAAAGGGAAATTCTATCAACGAGGGAGGTATCTTCAAGTCATTTGAATCCAAGGGACATGAGAAAAGGCAAGTTGTTGAACAATCTTCGGTATTGCAAGCTAATCCTGCAGCCTTTTCCCGGTCACTCAACTTCCGTGAGATCTCATGA

Protein Analysis

460

Amino Acids

49.53

Weight (kDa)

5.86

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 154 - 210 1.1e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1098
Acc16I TGCGCA 1 cut(s) 720
Acc36I ACCTGC 1 cut(s) 1098
AccB1I GGYRCC 1 cut(s) 64
AccI GTMKAC 1 cut(s) 969
AciI CCGC 2 cut(s) 104, 538
AclWI GGATC 2 cut(s) 925, 1175
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 3 cut(s) 867, 940, 1243
AcuI CTGAAG 3 cut(s) 237, 360, 467
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 2 cut(s) 852, 1019
AfiI CCNNNNNNNGG 5 cut(s) 74, 110, 734, 1144, 1353
AflIII ACRYGT 1 cut(s) 657
AgsI TTSAA 6 cut(s) 164, 310, 805, 1270, 1280, 1313
AjnI CCWGG 2 cut(s) 1029, 1158
AleI CACNNNNGTG 1 cut(s) 1065
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
Alw21I GWGCWC 1 cut(s) 1205
Alw26I GTCTC 2 cut(s) 2, 413
AlwI GGATC 2 cut(s) 925, 1175
AoxI GGCC 2 cut(s) 76, 1042
ApeKI GCWGC 7 cut(s) 149, 152, 155, 704, 1148, 1193, 1343
ApoI RAATTY 3 cut(s) 867, 940, 1243
AspLEI GCGC 2 cut(s) 67, 721
AspS9I GGNCC 2 cut(s) 1070, 1156
AsuC2I CCSGG 1 cut(s) 1354
AsuHPI GGTGA 1 cut(s) 1052
AsuII TTCGAA 1 cut(s) 938
AvaII GGWCC 2 cut(s) 1070, 1156
BaeI ACNNNNGTAYC 2 cut(s) 363, 396
BanI GGYRCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 1205
BbsI GAAGAC 2 cut(s) 225, 309
Bbv12I GWGCWC 1 cut(s) 1205
BbvCI CCTCAGC 1 cut(s) 358
BbvI GCAGC 7 cut(s) 136, 139, 142, 691, 1135, 1180, 1355
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 2 cut(s) 1031, 1160
BclI TGATCA 1 cut(s) 856
BcnI CCSGG 1 cut(s) 1354
BcoDI GTCTC 2 cut(s) 2, 413
BfaI CTAG 2 cut(s) 693, 1185
BfmI CTRYAG 1 cut(s) 1341
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 1098
BglII AGATCT 1 cut(s) 1374
BisI GCNGC 7 cut(s) 150, 153, 156, 705, 1149, 1194, 1344
BlsI GCNGC 7 cut(s) 151, 154, 157, 706, 1150, 1195, 1345
Bme1390I CCNGG 3 cut(s) 1031, 1160, 1354
Bme18I GGWCC 2 cut(s) 1070, 1156
BmgT120I GGNCC 2 cut(s) 1070, 1156
BmiI GGNNCC 4 cut(s) 66, 87, 876, 1035
BmrFI CCNGG 3 cut(s) 1031, 1160, 1354
BmrI ACTGGG 1 cut(s) 1152
BmsI GCATC 3 cut(s) 661, 946, 1233
BmuI ACTGGG 1 cut(s) 1152
BpiI GAAGAC 2 cut(s) 225, 309
BpmI CTGGAG 1 cut(s) 909
Bpu10I CCTNAGC 1 cut(s) 358
Bpu14I TTCGAA 1 cut(s) 938
BpuMI CCSGG 1 cut(s) 1354
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 4 cut(s) 54, 1039, 1051, 1284
Bsc4I CCNNNNNNNGG 5 cut(s) 74, 110, 734, 1144, 1353
Bse1I ACTGG 5 cut(s) 423, 572, 892, 1147, 1158
Bse3DI GCAATG 1 cut(s) 1085
BseBI CCWGG 2 cut(s) 1031, 1160
BseDI CCNNGG 4 cut(s) 54, 1039, 1051, 1284
BseGI GGATG 3 cut(s) 676, 907, 961
BseLI CCNNNNNNNGG 5 cut(s) 74, 110, 734, 1144, 1353
BseMI GCAATG 1 cut(s) 1085
BseMII CTCAG 3 cut(s) 192, 372, 1078
BseNI ACTGG 5 cut(s) 423, 572, 892, 1147, 1158
BseRI GAGGAG 2 cut(s) 765, 998
BseXI GCAGC 7 cut(s) 136, 139, 142, 691, 1135, 1180, 1355
BsgI GTGCAG 1 cut(s) 1134
BshFI GGCC 2 cut(s) 78, 1044
BshNI GGYRCC 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 1205
BsiSI CCGG 2 cut(s) 576, 1354
BslFI GGGAC 2 cut(s) 459, 1302
BslI CCNNNNNNNGG 5 cut(s) 74, 110, 734, 1144, 1353
BsmAI GTCTC 2 cut(s) 2, 413
BsmFI GGGAC 2 cut(s) 459, 1302
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 2 cut(s) 78, 1044
Bso31I GGTCTC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 938
Bsp1286I GDGCHC 1 cut(s) 1205
Bsp143I GATC 5 cut(s) 289, 856, 917, 1180, 1374
Bsp19I CCATGG 1 cut(s) 1051
BspACI CCGC 2 cut(s) 104, 538
BspANI GGCC 2 cut(s) 78, 1044
BspCNI CTCAG 3 cut(s) 191, 371, 1077
BspHI TCATGA 2 cut(s) 585, 1379
BspLI GGNNCC 4 cut(s) 66, 87, 876, 1035
BspMAI CTGCAG 1 cut(s) 1345
BspMI ACCTGC 1 cut(s) 1098
BspPI GGATC 2 cut(s) 925, 1175
BspQI GCTCTTC 1 cut(s) 41
BspT104I TTCGAA 1 cut(s) 938
BspT107I GGYRCC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 1085
BsrI ACTGG 5 cut(s) 423, 572, 892, 1147, 1158
BssECI CCNNGG 4 cut(s) 54, 1039, 1051, 1284
BssMI GATC 5 cut(s) 289, 856, 917, 1180, 1374
BssNAI GTATAC 1 cut(s) 970
BssNI GRCGYC 1 cut(s) 65
BssT1I CCWWGG 4 cut(s) 54, 1039, 1051, 1284
Bst1107I GTATAC 1 cut(s) 970
Bst2UI CCWGG 2 cut(s) 1031, 1160
Bst4CI ACNGT 4 cut(s) 125, 557, 751, 994
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 938
BstC8I GCNNGC 1 cut(s) 1332
BstDEI CTNAG 7 cut(s) 6, 138, 178, 286, 358, 396, 1064
BstDSI CCRYGG 1 cut(s) 1051
BstF5I GGATG 3 cut(s) 676, 907, 961
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 2 cut(s) 67, 721
BstKTI GATC 5 cut(s) 292, 859, 920, 1183, 1377
BstMAI GTCTC 2 cut(s) 2, 413
BstMBI GATC 5 cut(s) 289, 856, 917, 1180, 1374
BstMWI GCNNNNNNNGC 6 cut(s) 155, 546, 638, 704, 1031, 1340
BstNI CCWGG 2 cut(s) 1031, 1160
BstNSI RCATGY 1 cut(s) 661
BstSCI CCNGG 3 cut(s) 1029, 1158, 1352
BstSFI CTRYAG 1 cut(s) 1341
BstV1I GCAGC 7 cut(s) 136, 139, 142, 691, 1135, 1180, 1355
BstV2I GAAGAC 2 cut(s) 225, 309
BstX2I RGATCY 1 cut(s) 1374
BstXI CCANNNNNNTGG 2 cut(s) 55, 914
BstYI RGATCY 1 cut(s) 1374
BstZ17I GTATAC 1 cut(s) 970
BsuRI GGCC 2 cut(s) 78, 1044
BtgI CCRYGG 1 cut(s) 1051
BtsCI GGATG 3 cut(s) 676, 907, 961
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 4 cut(s) 258, 747, 1072, 1151
BveI ACCTGC 1 cut(s) 1098
Cac8I GCNNGC 1 cut(s) 1332
CciI TCATGA 2 cut(s) 585, 1379
CfoI GCGC 2 cut(s) 67, 721
Cfr13I GGNCC 2 cut(s) 1070, 1156
Csp6I GTAC 2 cut(s) 851, 1018
CviQI GTAC 2 cut(s) 851, 1018
DdeI CTNAG 7 cut(s) 6, 138, 178, 286, 358, 396, 1064
DinI GGCGCC 1 cut(s) 66
DpnI GATC 5 cut(s) 291, 858, 919, 1182, 1376
DpnII GATC 5 cut(s) 289, 856, 917, 1180, 1374
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 1203
Eco130I CCWWGG 4 cut(s) 54, 1039, 1051, 1284
Eco24I GRGCYC 1 cut(s) 1205
Eco31I GGTCTC 1 cut(s) 413
Eco47I GGWCC 2 cut(s) 1070, 1156
Eco53kI GAGCTC 1 cut(s) 1203
Eco57I CTGAAG 3 cut(s) 237, 360, 467
EcoICRI GAGCTC 1 cut(s) 1203
EcoRI GAATTC 2 cut(s) 867, 940
EcoRII CCWGG 2 cut(s) 1029, 1158
EcoT14I CCWWGG 4 cut(s) 54, 1039, 1051, 1284
EcoT38I GRGCYC 1 cut(s) 1205
EgeI GGCGCC 1 cut(s) 66
EheI GGCGCC 1 cut(s) 66
ErhI CCWWGG 4 cut(s) 54, 1039, 1051, 1284
FaqI GGGAC 2 cut(s) 459, 1302
FauI CCCGC 2 cut(s) 111, 545
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 1 cut(s) 969
Fnu4HI GCNGC 7 cut(s) 150, 153, 156, 705, 1149, 1194, 1344
FokI GGATG 3 cut(s) 683, 914, 968
FriOI GRGCYC 1 cut(s) 1205
Fsp4HI GCNGC 7 cut(s) 150, 153, 156, 705, 1149, 1194, 1344
FspAI RTGCGCAY 1 cut(s) 720
FspBI CTAG 2 cut(s) 693, 1185
FspI TGCGCA 1 cut(s) 720
GlaI GCGC 2 cut(s) 66, 720
GluI GCNGC 7 cut(s) 150, 153, 156, 705, 1149, 1194, 1344
GsuI CTGGAG 1 cut(s) 909
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 2 cut(s) 78, 1044
HapII CCGG 2 cut(s) 576, 1354
HhaI GCGC 2 cut(s) 67, 721
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 2 cut(s) 65, 719
HinP1I GCGC 2 cut(s) 65, 719
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 2 cut(s) 185, 999
HinfI GANTC 7 cut(s) 134, 343, 442, 582, 770, 785, 1280
HpaII CCGG 2 cut(s) 576, 1354
HphI GGTGA 1 cut(s) 1052
Hpy166II GTNNAC 4 cut(s) 133, 970, 987, 1070
Hpy188III TCNNGA 4 cut(s) 330, 586, 1228, 1380
Hpy8I GTNNAC 4 cut(s) 133, 970, 987, 1070
Hpy99I CGWCG 2 cut(s) 331, 618
HpyAV CCTTC 1 cut(s) 888
HpyCH4III ACNGT 4 cut(s) 125, 557, 751, 994
HpyCH4V TGCA 9 cut(s) 549, 959, 1078, 1093, 1132, 1151, 1196, 1330, 1343
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 546, 638, 704, 1031, 1340
HpyF3I CTNAG 7 cut(s) 6, 138, 178, 286, 358, 396, 1064
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 2 cut(s) 65, 719
KasI GGCGCC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 856
Kzo9I GATC 5 cut(s) 289, 856, 917, 1180, 1374
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 3 cut(s) 113, 605, 1039
Lsp1109I GCAGC 7 cut(s) 136, 139, 142, 691, 1135, 1180, 1355
LweI GCATC 3 cut(s) 661, 946, 1233
MaeI CTAG 2 cut(s) 693, 1185
MaeIII GTNAC 4 cut(s) 506, 653, 928, 1356
MalI GATC 5 cut(s) 291, 858, 919, 1182, 1376
MboI GATC 5 cut(s) 289, 856, 917, 1180, 1374
MflI RGATCY 1 cut(s) 1374
MhlI GDGCHC 1 cut(s) 1205
MluCI AATT 5 cut(s) 280, 333, 867, 940, 1243
Mly113I GGCGCC 1 cut(s) 65
MlyI GAGTC 2 cut(s) 128, 436
MmeI TCCRAC 5 cut(s) 113, 144, 341, 648, 752
MseI TTAA 1 cut(s) 662
MslI CAYNNNNRTG 3 cut(s) 662, 693, 1065
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 2 cut(s) 576, 1354
MspR9I CCNGG 3 cut(s) 1031, 1160, 1354
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 2 cut(s) 1031, 1160
MwoI GCNNNNNNNGC 6 cut(s) 155, 546, 638, 704, 1031, 1340
NarI GGCGCC 1 cut(s) 65
NciI CCSGG 1 cut(s) 1354
NcoI CCATGG 1 cut(s) 1051
NdeII GATC 5 cut(s) 289, 856, 917, 1180, 1374
NlaIV GGNNCC 4 cut(s) 66, 87, 876, 1035
NmeAIII GCCGAG 1 cut(s) 838
NmuCI GTSAC 2 cut(s) 653, 1356
NsbI TGCGCA 1 cut(s) 720
NspI RCATGY 1 cut(s) 661
NspV TTCGAA 1 cut(s) 938
OliI CACNNNNGTG 1 cut(s) 1065
PagI TCATGA 2 cut(s) 585, 1379
PaqCI CACCTGC 1 cut(s) 1098
PciI ACATGT 1 cut(s) 657
PciSI GCTCTTC 1 cut(s) 41
PctI GAATGC 1 cut(s) 1093
PfeI GAWTC 5 cut(s) 343, 582, 770, 785, 1280
PkrI GCNGC 7 cut(s) 151, 154, 157, 706, 1150, 1195, 1345
PleI GAGTC 2 cut(s) 128, 436
PluTI GGCGCC 1 cut(s) 68
PpsI GAGTC 2 cut(s) 128, 436
PscI ACATGT 1 cut(s) 657
Psp124BI GAGCTC 1 cut(s) 1205
Psp6I CCWGG 2 cut(s) 1029, 1158
PspGI CCWGG 2 cut(s) 1029, 1158
PspN4I GGNNCC 4 cut(s) 66, 87, 876, 1035
PspPI GGNCC 2 cut(s) 1070, 1156
PstI CTGCAG 1 cut(s) 1345
PsuI RGATCY 1 cut(s) 1374
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 2 cut(s) 852, 1019
RsaNI GTAC 2 cut(s) 851, 1018
RseI CAYNNNNRTG 3 cut(s) 662, 693, 1065
SacI GAGCTC 1 cut(s) 1205
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 662
SatI GCNGC 7 cut(s) 150, 153, 156, 705, 1149, 1194, 1344
Sau3AI GATC 5 cut(s) 289, 856, 917, 1180, 1374
Sau96I GGNCC 2 cut(s) 1070, 1156
SchI GAGTC 2 cut(s) 128, 436
ScrFI CCNGG 3 cut(s) 1031, 1160, 1354
SduI GDGCHC 1 cut(s) 1205
SfaNI GCATC 3 cut(s) 661, 946, 1233
SfcI CTRYAG 1 cut(s) 1341
SfoI GGCGCC 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 938
SinI GGWCC 2 cut(s) 1070, 1156
SmiMI CAYNNNNRTG 3 cut(s) 662, 693, 1065
Sse9I AATT 5 cut(s) 280, 333, 867, 940, 1243
SsiI CCGC 2 cut(s) 104, 538
SspDI GGCGCC 1 cut(s) 64
SspMI CTAG 2 cut(s) 693, 1185
SstI GAGCTC 1 cut(s) 1205
StyD4I CCNGG 3 cut(s) 1029, 1158, 1352
StyI CCWWGG 4 cut(s) 54, 1039, 1051, 1284
TaaI ACNGT 4 cut(s) 125, 557, 751, 994
TaqI TCGA 1 cut(s) 938
TasI AATT 5 cut(s) 280, 333, 867, 940, 1243
TfiI GAWTC 5 cut(s) 343, 582, 770, 785, 1280
Tru1I TTAA 1 cut(s) 662
Tru9I TTAA 1 cut(s) 662
TscAI CASTG 4 cut(s) 265, 754, 1072, 1158
TseFI GTSAC 2 cut(s) 653, 1356
TseI GCWGC 7 cut(s) 149, 152, 155, 704, 1148, 1193, 1343
Tsp45I GTSAC 2 cut(s) 653, 1356
TspDTI ATGAA 4 cut(s) 624, 836, 933, 1248
TspGWI ACGGA 1 cut(s) 1358
TspRI CASTG 4 cut(s) 265, 754, 1072, 1158
VpaK11BI GGWCC 2 cut(s) 1070, 1156
XapI RAATTY 3 cut(s) 867, 940, 1243
XceI RCATGY 1 cut(s) 661
XcmI CCANNNNNNNNNTGG 1 cut(s) 1037
XmiI GTMKAC 1 cut(s) 969
XspI CTAG 2 cut(s) 693, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.