Rroxscaffold_2G00083270

Cyclic dof factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003750
Physical Location & Seq
Forward (+)
5941224 .. 5943805
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083270.1

Sequence Viewer

Length: 1347 bp
ATGTCGGAGGGGAGGGACCCGGCGATCAAGCTTTTCGGGAAGACGATTCCGCTGCCGGAGGTCCCGCCGTTGCTGAGAGAATCCACCGTCCCGGCGGCGGCGGATGAAGTCGTCGGAGCTCCGGATCCGGGTTCGGATGCTGACCGTGTTTCGTCTTCGTCCAACTCGTCCTCCAGGGGAGGAGGAGGAGGAGGAGGAGGAGAAGTCAATGATAATAAAGACGGTGAAGAAGAGAAAGAGGTCAATAAGGACATATTGGGAGAAAAAGCAATTCAGAATAAAGAGGGAGATGAAGTCCTACCTTTATCTTCTGGAGAGTTGATGAATCCAGACGCAGCATCAAGAATAGGTGAAAGTCCTAAAGCACCAGTTGACAAGGAGACTGCTGCACTCAAAACTTTGAAGGCGGAAGAAGAACAGAGCGAGACAAGTAATTCACAAGAAAAGACCCTGAAGAAGCCAGACAAAATTATTCCATGCCCCCGCTGTAATAGCATGGACACCAAGTTCTGTTACTACAACAACTACAATGTCAACCAACCCCGGCACTTCTGCAAAAACTGCCAGAGATATTGGACAGCTGGTGGGACTATGAGAAATGTGCCAGTGGGTGCTGGTCGTCGTAAGAATAAGAACACCCCTTCTCAGTATCGCCACATAACTGTCTCTGAAGCTCTCCCGAATGCTCGACCTGATGTTCTAAATGGAGTCCACCATCCGTCAGTACAATCTAATGGCACTGTCCTGACATTTTGCTCAGACACGCCCCTTTGCGAGTCAATGACCTCTGTTCTTCAGCTTGCTGATAAGAACGTACAGAACCGTACTCCCAATGGATTTCACAAACCAGAAGGATTAATTGTTCCAGCTTCTTATGGGAGCGGAGTAAATGGAGATGATCATTCGAATAGGTCTACGGTCACAGATTCAAATTCAAAGGGACGAGACATCCAAACCCGGGCCACAAGAGCAAGTTATGCAGAATTATCCTGGCTTCCCGCCTCAAATACCATGCTTTCCGGGTGCTCCTTGGCCTTACCCGTGGAACCCTGCTCAATGGAGCTCTCCACTGCTTATTGGGGTTGTGCTGTACAAGGCACTTGGAACATGCCTTGGCTTCCTCAGCCATCATCTCCAAACCAAACTGCTCCAAGTTCTGGTTCTAACTCTCCGACTTTGGGGAAACATTCAAGGGATGAGAACCCTCTGAAACAAACCAGCTCTGAGGAAGAGGAGACAACAAAAGAAAAGAATGCCGAGAGATGCCTTTGGATTCCGAAAACATTGAGGATTGATGACCCAGGAGAAGCAGCAAGAAAGCTCTATATGGGCAACATTGGGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

448

Amino Acids

48.34

Weight (kDa)

5.59

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 156 - 212 9.8e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1157
AccBSI CCGCTC 1 cut(s) 882
AccI GTMKAC 1 cut(s) 914
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 9 cut(s) 50, 65, 95, 98, 101, 407, 484, 882, 999
AclWI GGATC 2 cut(s) 119, 132
AcsI RAATTY 1 cut(s) 931
AcuI CTGAAG 3 cut(s) 473, 690, 779
AfaI GTAC 4 cut(s) 726, 816, 826, 1092
AfiI CCNNNNNNNGG 5 cut(s) 97, 128, 958, 1157, 1178
AgsI TTSAA 4 cut(s) 403, 930, 936, 1191
AjnI CCWGG 3 cut(s) 173, 989, 1300
AjuI GAANNNNNNNTTGG 2 cut(s) 1144, 1176
AloI GAACNNNNNNTCC 4 cut(s) 491, 523, 846, 878
AluBI AGCT 9 cut(s) 31, 119, 581, 674, 799, 869, 1063, 1221, 1321
AluI AGCT 9 cut(s) 31, 119, 581, 674, 799, 869, 1063, 1221, 1321
Alw21I GWGCWC 3 cut(s) 121, 1028, 1065
Alw26I GTCTC 5 cut(s) 374, 419, 670, 939, 1229
AlwI GGATC 2 cut(s) 119, 132
Ama87I CYCGRG 1 cut(s) 957
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 2 cut(s) 960, 1032
ApeKI GCWGC 4 cut(s) 52, 335, 386, 1310
ApoI RAATTY 1 cut(s) 931
AseI ATTAAT 1 cut(s) 857
AspS9I GGNCC 3 cut(s) 16, 61, 960
AsuC2I CCSGG 7 cut(s) 20, 92, 129, 544, 958, 959, 1021
AsuHPI GGTGA 2 cut(s) 236, 362
AsuII TTCGAA 1 cut(s) 905
AvaI CYCGRG 1 cut(s) 957
AvaII GGWCC 2 cut(s) 16, 61
BamHI GGATCC 1 cut(s) 124
BanII GRGCYC 2 cut(s) 121, 1065
BbsI GAAGAC 2 cut(s) 47, 147
Bbv12I GWGCWC 3 cut(s) 121, 1028, 1065
BbvCI CCTCAGC 1 cut(s) 1122
BbvI GCAGC 4 cut(s) 39, 347, 373, 1322
BccI CCATC 2 cut(s) 723, 1135
BceAI ACGGC 1 cut(s) 52
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BciT130I CCWGG 3 cut(s) 175, 991, 1302
BclI TGATCA 1 cut(s) 898
BcnI CCSGG 7 cut(s) 20, 92, 129, 544, 958, 959, 1021
BcoDI GTCTC 5 cut(s) 374, 419, 670, 939, 1229
BisI GCNGC 6 cut(s) 53, 96, 99, 336, 387, 1311
BlsI GCNGC 6 cut(s) 54, 97, 100, 337, 388, 1312
Bme18I GGWCC 2 cut(s) 16, 61
BmeT110I CYCGRG 1 cut(s) 957
BmgT120I GGNCC 3 cut(s) 16, 61, 960
BmiI GGNNCC 5 cut(s) 17, 18, 63, 126, 1047
BmsI GCATC 3 cut(s) 127, 347, 1253
BpiI GAAGAC 2 cut(s) 47, 147
BpmI CTGGAG 2 cut(s) 157, 333
Bpu10I CCTNAGC 1 cut(s) 1122
Bpu14I TTCGAA 1 cut(s) 905
BpuMI CCSGG 7 cut(s) 20, 92, 129, 544, 958, 959, 1021
BsaJI CCNNGG 7 cut(s) 174, 542, 957, 1029, 1041, 1112, 1300
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 189, 219, 279, 309
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 128, 958, 1157, 1178
Bse1I ACTGG 2 cut(s) 368, 605
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 3 cut(s) 175, 991, 1302
BseDI CCNNGG 7 cut(s) 174, 542, 957, 1029, 1041, 1112, 1300
BseGI GGATG 5 cut(s) 109, 142, 715, 948, 1201
BseLI CCNNNNNNNGG 5 cut(s) 97, 128, 958, 1157, 1178
BseMII CTCAG 5 cut(s) 65, 659, 771, 1136, 1215
BseNI ACTGG 2 cut(s) 368, 605
BseRI GAGGAG 8 cut(s) 195, 198, 201, 204, 207, 210, 213, 1247
BseXI GCAGC 4 cut(s) 39, 347, 373, 1322
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 2 cut(s) 962, 1034
BsiHKAI GWGCWC 3 cut(s) 121, 1028, 1065
BsiHKCI CYCGRG 1 cut(s) 957
BsiSI CCGG 8 cut(s) 20, 56, 92, 122, 128, 544, 958, 1020
BslFI GGGAC 5 cut(s) 29, 47, 74, 601, 954
BslI CCNNNNNNNGG 5 cut(s) 97, 128, 958, 1157, 1178
BsmAI GTCTC 5 cut(s) 374, 419, 670, 939, 1229
BsmFI GGGAC 5 cut(s) 29, 47, 74, 601, 954
BsmI GAATGC 2 cut(s) 688, 1258
BsnI GGCC 2 cut(s) 962, 1034
BsoBI CYCGRG 1 cut(s) 957
Bsp119I TTCGAA 1 cut(s) 905
Bsp1286I GDGCHC 3 cut(s) 121, 1028, 1065
Bsp13I TCCGGA 1 cut(s) 121
Bsp1407I TGTACA 1 cut(s) 1090
Bsp143I GATC 3 cut(s) 24, 124, 898
BspACI CCGC 9 cut(s) 50, 65, 95, 98, 101, 407, 484, 882, 999
BspANI GGCC 2 cut(s) 962, 1034
BspCNI CTCAG 5 cut(s) 66, 658, 770, 1135, 1216
BspEI TCCGGA 1 cut(s) 121
BspLI GGNNCC 5 cut(s) 17, 18, 63, 126, 1047
BspPI GGATC 2 cut(s) 119, 132
BspT104I TTCGAA 1 cut(s) 905
BsrBI CCGCTC 1 cut(s) 882
BsrGI TGTACA 1 cut(s) 1090
BsrI ACTGG 2 cut(s) 368, 605
BssECI CCNNGG 7 cut(s) 174, 542, 957, 1029, 1041, 1112, 1300
BssMI GATC 3 cut(s) 24, 124, 898
BssT1I CCWWGG 2 cut(s) 1029, 1112
Bst2UI CCWGG 3 cut(s) 175, 991, 1302
Bst4CI ACNGT 7 cut(s) 88, 146, 224, 664, 742, 824, 919
Bst6I CTCTTC 2 cut(s) 225, 1224
BstAPI GCANNNNNTGC 2 cut(s) 561, 977
BstAUI TGTACA 1 cut(s) 1090
BstBI TTCGAA 1 cut(s) 905
BstC8I GCNNGC 1 cut(s) 801
BstDEI CTNAG 5 cut(s) 74, 645, 757, 1122, 1224
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 5 cut(s) 109, 142, 715, 948, 1201
BstKTI GATC 3 cut(s) 27, 127, 901
BstMAI GTCTC 5 cut(s) 374, 419, 670, 939, 1229
BstMBI GATC 3 cut(s) 24, 124, 898
BstMWI GCNNNNNNNGC 5 cut(s) 492, 561, 968, 977, 1123
BstNI CCWGG 3 cut(s) 175, 991, 1302
BstNSI RCATGY 1 cut(s) 1111
BstV1I GCAGC 4 cut(s) 39, 347, 373, 1322
BstV2I GAAGAC 2 cut(s) 47, 147
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 2 cut(s) 962, 1034
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 5 cut(s) 109, 142, 715, 948, 1201
BtsI GCAGTG 1 cut(s) 1068
BtsIMutI CAGTG 3 cut(s) 612, 738, 1068
Cac8I GCNNGC 1 cut(s) 801
Cfr13I GGNCC 3 cut(s) 16, 61, 960
Cfr9I CCCGGG 1 cut(s) 957
CseI GACGC 1 cut(s) 341
Csp6I GTAC 4 cut(s) 725, 815, 825, 1091
CviAII CATG 4 cut(s) 477, 496, 1012, 1108
CviQI GTAC 4 cut(s) 725, 815, 825, 1091
DdeI CTNAG 5 cut(s) 74, 645, 757, 1122, 1224
DpnI GATC 3 cut(s) 26, 126, 900
DpnII GATC 3 cut(s) 24, 124, 898
Eam1104I CTCTTC 2 cut(s) 225, 1224
EarI CTCTTC 2 cut(s) 225, 1224
EciI GGCGGA 2 cut(s) 116, 422
Ecl136II GAGCTC 2 cut(s) 119, 1063
Eco130I CCWWGG 2 cut(s) 1029, 1112
Eco24I GRGCYC 2 cut(s) 121, 1065
Eco47I GGWCC 2 cut(s) 16, 61
Eco53kI GAGCTC 2 cut(s) 119, 1063
Eco57I CTGAAG 3 cut(s) 473, 690, 779
Eco88I CYCGRG 1 cut(s) 957
EcoICRI GAGCTC 2 cut(s) 119, 1063
EcoO109I RGGNCCY 2 cut(s) 16, 61
EcoRII CCWGG 3 cut(s) 173, 989, 1300
EcoT14I CCWWGG 2 cut(s) 1029, 1112
EcoT38I GRGCYC 2 cut(s) 121, 1065
ErhI CCWWGG 2 cut(s) 1029, 1112
FaeI CATG 4 cut(s) 480, 499, 1015, 1111
FaqI GGGAC 5 cut(s) 29, 47, 74, 601, 954
FatI CATG 4 cut(s) 476, 495, 1011, 1107
FauI CCCGC 3 cut(s) 72, 491, 1006
FbaI TGATCA 1 cut(s) 898
FblI GTMKAC 1 cut(s) 914
Fnu4HI GCNGC 6 cut(s) 53, 96, 99, 336, 387, 1311
FokI GGATG 5 cut(s) 116, 149, 702, 935, 1208
FriOI GRGCYC 2 cut(s) 121, 1065
Fsp4HI GCNGC 6 cut(s) 53, 96, 99, 336, 387, 1311
GluI GCNGC 6 cut(s) 53, 96, 99, 336, 387, 1311
GsuI CTGGAG 2 cut(s) 157, 333
HaeIII GGCC 2 cut(s) 962, 1034
HapII CCGG 8 cut(s) 20, 56, 92, 122, 128, 544, 958, 1020
HgaI GACGC 1 cut(s) 341
Hin1II CATG 4 cut(s) 480, 499, 1015, 1111
HincII GTYRAC 2 cut(s) 373, 535
HindII GTYRAC 2 cut(s) 373, 535
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 7 cut(s) 46, 80, 325, 708, 776, 926, 1273
HpaII CCGG 8 cut(s) 20, 56, 92, 122, 128, 544, 958, 1020
HphI GGTGA 2 cut(s) 236, 362
Hpy166II GTNNAC 4 cut(s) 373, 535, 712, 915
Hpy188III TCNNGA 7 cut(s) 37, 122, 312, 329, 342, 679, 745
Hpy8I GTNNAC 4 cut(s) 373, 535, 712, 915
Hpy99I CGWCG 2 cut(s) 116, 624
HpyAV CCTTC 3 cut(s) 397, 651, 845
HpyCH4III ACNGT 7 cut(s) 88, 146, 224, 664, 742, 824, 919
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 3 cut(s) 389, 555, 980
HpyF10VI GCNNNNNNNGC 5 cut(s) 492, 561, 968, 977, 1123
HpyF3I CTNAG 5 cut(s) 74, 645, 757, 1122, 1224
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 4 cut(s) 480, 499, 1015, 1111
KflI GGGWCCC 1 cut(s) 16
Kpn2I TCCGGA 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 3 cut(s) 24, 124, 898
LmnI GCTCC 6 cut(s) 116, 124, 879, 1031, 1060, 1153
Lsp1109I GCAGC 4 cut(s) 39, 347, 373, 1322
LweI GCATC 3 cut(s) 127, 347, 1253
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 2 cut(s) 512, 919
MalI GATC 3 cut(s) 26, 126, 900
MbiI CCGCTC 1 cut(s) 882
MboI GATC 3 cut(s) 24, 124, 898
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 3 cut(s) 121, 1028, 1065
MluCI AATT 6 cut(s) 270, 433, 468, 858, 931, 983
MlyI GAGTC 2 cut(s) 717, 785
MmeI TCCRAC 3 cut(s) 94, 186, 1196
MroI TCCGGA 1 cut(s) 121
MseI TTAA 2 cut(s) 857, 1345
MspA1I CMGCKG 3 cut(s) 52, 486, 581
MspI CCGG 8 cut(s) 20, 56, 92, 122, 128, 544, 958, 1020
Mva1269I GAATGC 2 cut(s) 688, 1258
MvaI CCWGG 3 cut(s) 175, 991, 1302
MwoI GCNNNNNNNGC 5 cut(s) 492, 561, 968, 977, 1123
NciI CCSGG 7 cut(s) 20, 92, 129, 544, 958, 959, 1021
NdeII GATC 3 cut(s) 24, 124, 898
NlaIII CATG 4 cut(s) 480, 499, 1015, 1111
NlaIV GGNNCC 5 cut(s) 17, 18, 63, 126, 1047
NmeAIII GCCGAG 1 cut(s) 1282
NmuCI GTSAC 1 cut(s) 919
NspI RCATGY 1 cut(s) 1111
NspV TTCGAA 1 cut(s) 905
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PctI GAATGC 2 cut(s) 688, 1258
PfeI GAWTC 5 cut(s) 46, 80, 325, 926, 1273
PflMI CCANNNNNTGG 1 cut(s) 1157
PkrI GCNGC 6 cut(s) 54, 97, 100, 337, 388, 1312
PleI GAGTC 2 cut(s) 716, 784
PpsI GAGTC 2 cut(s) 716, 784
PpuMI RGGWCCY 2 cut(s) 16, 61
PshBI ATTAAT 1 cut(s) 857
Psp124BI GAGCTC 2 cut(s) 121, 1065
Psp5II RGGWCCY 2 cut(s) 16, 61
Psp6I CCWGG 3 cut(s) 173, 989, 1300
PspGI CCWGG 3 cut(s) 173, 989, 1300
PspN4I GGNNCC 5 cut(s) 17, 18, 63, 126, 1047
PspPI GGNCC 3 cut(s) 16, 61, 960
PspPPI RGGWCCY 2 cut(s) 16, 61
PsuI RGATCY 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 4 cut(s) 726, 816, 826, 1092
RsaNI GTAC 4 cut(s) 725, 815, 825, 1091
SacI GAGCTC 2 cut(s) 121, 1065
SaqAI TTAA 2 cut(s) 857, 1345
SatI GCNGC 6 cut(s) 53, 96, 99, 336, 387, 1311
Sau3AI GATC 3 cut(s) 24, 124, 898
Sau96I GGNCC 3 cut(s) 16, 61, 960
SchI GAGTC 2 cut(s) 717, 785
SduI GDGCHC 3 cut(s) 121, 1028, 1065
SfaNI GCATC 3 cut(s) 127, 347, 1253
SfuI TTCGAA 1 cut(s) 905
SinI GGWCC 2 cut(s) 16, 61
SmaI CCCGGG 1 cut(s) 959
Sse9I AATT 6 cut(s) 270, 433, 468, 858, 931, 983
SsiI CCGC 9 cut(s) 50, 65, 95, 98, 101, 407, 484, 882, 999
SstI GAGCTC 2 cut(s) 121, 1065
StyI CCWWGG 2 cut(s) 1029, 1112
TaaI ACNGT 7 cut(s) 88, 146, 224, 664, 742, 824, 919
TaiI ACGT 1 cut(s) 816
TaqI TCGA 2 cut(s) 688, 905
TasI AATT 6 cut(s) 270, 433, 468, 858, 931, 983
TatI WGTACW 2 cut(s) 724, 1090
TauI GCSGC 2 cut(s) 98, 101
TfiI GAWTC 5 cut(s) 46, 80, 325, 926, 1273
Tru1I TTAA 2 cut(s) 857, 1345
Tru9I TTAA 2 cut(s) 857, 1345
TscAI CASTG 3 cut(s) 612, 745, 1075
TseFI GTSAC 1 cut(s) 919
TseI GCWGC 4 cut(s) 52, 335, 386, 1310
Tsp45I GTSAC 1 cut(s) 919
TspDTI ATGAA 3 cut(s) 120, 306, 338
TspGWI ACGGA 1 cut(s) 708
TspMI CCCGGG 1 cut(s) 957
TspRI CASTG 3 cut(s) 612, 745, 1075
Van91I CCANNNNNTGG 1 cut(s) 1157
VpaK11BI GGWCC 2 cut(s) 16, 61
VspI ATTAAT 1 cut(s) 857
XapI RAATTY 1 cut(s) 931
XceI RCATGY 1 cut(s) 1111
XmaI CCCGGG 1 cut(s) 957
XmiI GTMKAC 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.