RchiOBHm_Chr4g0439611

Cyclic dof factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003750
Physical Location & Seq
Reverse (-)
62018009 .. 62021426
3418 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40765

Sequence Viewer

Length: 1596 bp
ATGTCTGAGAATGAAATCAGCCCCACCATAAAGCTCTTCGGAAAGACCATACCTTTGGCTTCGGCGCCACACAACGGCCATGAGGTTCCGACCAATGATGACCCGCCATGTGGAGCTTCCGACTCTGCTGTTGACTCTAAGATTTCTGCAGCTGCTGCTGCTGCTGCTGCTTCCGGTGGTGGTGCTGTTGAAGAAGAAGATTGCTCAGTCAAGCTTCCTTGTTGTTCAGAAACTACTTCGCCTGAAGACCAAGAGAACAAGGAAACAATATCAGGCAAAGAATTCACTGCTGACAAAGAGGAAGATAATCCCTTAGATCAATTCACAGAAGACTTGAAACCTCCAACAACATCGTCGGGAATTAGTGAGAATCCCAAAACTCCCTCAGCTGATAGAGAAACTTCAGTATCTCTGAAATCTTCTAAGAATGGAGAACAGAGTGATGAGACCAGTTGCTCACAAGACAAGACTCTGAAGAAGCCAGACAAGATACTTCCATGTCCCCGATGTAATAGCATGGACACCAAGTTCTGTTACTACAATAACTACAACGTCAATCAGCCCCGCCATTTTTGCAAGAACTGTCAAAGATACTGGACTGCCGGAGGAATCATGAGGAATGTTCCTGTGGGAGCTGGTCGTCGCAAGAACAAGAACGCTTCATCAGCTTCACACTATCGTCACATGTTAATGTCGGATGCTCTCCAAACAGCTCATGCGACTGCTGCTGCTGCTTCTAATGGCGCTCACAATCCAACATTGGGAAGAAATGGCACTGTCCTTACCTTTGGTTCAGATTCTCCTCTTTGTGAATCAATGGCTTCTGTATTGAACCTTGCTGAGAAAACACAAAACTCTCTTCAGAATGGTTTTCATGTACCTGATCAACAAAGAATTCTGGTTCCTTCCATAACTGGAGATAATGGGGATGACCAATCAAGTGGATCGTCTAATGTTACAGCTTCGAATTCATCAGAGGGGGGATGCAAAGCTGGTATACAAGAACCAGCGGTTCACAACTGTCAAAGCTTCTCTCCTCATCAAGTACCATGCTTTCCAGGCTCCCCTTGGCCTTATCCATGGAACTCACCTCAGTGGACCTCTGCAATGCCTCCGCCTGCATTCTTCCATTCAGGGTTTCCCATATCATTCTACCCTGCACCACCATACTGGGGCTGCACTGGACCCGGCTCTTGGAATGTACCATGTAATTCTCCACCATCCTCCAATCTTAGCCACTGTGGTACAAGCTCTGGTCCAAATTCCCCGACCTTAGGGAAACATCCAAGAGACGGGGACATTCTTAGTCAAGCCAGCTCTCAAAAAGAAGAGTCCAGCAGAGATAACAGGTCAGATAGATGTGTTTTGATTCCGAAAACGTTAAGGATTGATGATCCTAGTGAAGCTGCAAAGAGCTCTATATGGGAAACACTTGGCATCAAGAATGAAAAGGGAAATTCTATCAACGAGGGAGGTATCTTCAAGTCATTTGAATCCAAGGGACACGAGAAAAGGCAAGTTGTTGAACAATCTTCGGTATTGCAAGCTAATCCTGCAGCCTTTTCCCGGTCACTCAACTTCCGTGAGATCTCATGA

Protein Analysis

531

Amino Acids

56.83

Weight (kDa)

5.73

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 163 - 219 1.4e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 64
AccI GTMKAC 1 cut(s) 997
AciI CCGC 4 cut(s) 104, 565, 1010, 1115
AclI AACGTT 1 cut(s) 1379
AclWI GGATC 2 cut(s) 952, 1388
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 5 cut(s) 281, 894, 967, 1261, 1456
AcuI CTGAAG 4 cut(s) 264, 387, 494, 845
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 4 cut(s) 879, 1047, 1203, 1246
AfiI CCNNNNNNNGG 8 cut(s) 74, 110, 761, 1172, 1194, 1274, 1292, 1566
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 6 cut(s) 191, 337, 832, 1483, 1493, 1526
AjnI CCWGG 1 cut(s) 1057
AleI CACNNNNGTG 1 cut(s) 1093
AloI GAACNNNNNNTCC 2 cut(s) 512, 544
Alw21I GWGCWC 1 cut(s) 1418
Alw26I GTCTC 2 cut(s) 440, 1284
AlwI GGATC 2 cut(s) 952, 1388
AlwNI CAGNNNCTG 1 cut(s) 155
AoxI GGCC 2 cut(s) 76, 1070
ApoI RAATTY 5 cut(s) 281, 894, 967, 1261, 1456
ArsI GACNNNNNNTTYG 2 cut(s) 37, 69
AspLEI GCGC 2 cut(s) 67, 746
AspS9I GGNCC 3 cut(s) 1098, 1184, 1256
AsuC2I CCSGG 2 cut(s) 1188, 1567
AsuHPI GGTGA 1 cut(s) 1080
AsuII TTCGAA 1 cut(s) 965
AvaII GGWCC 3 cut(s) 1098, 1184, 1256
AxyI CCTNAGG 1 cut(s) 1273
BaeI ACNNNNGTAYC 2 cut(s) 390, 423
BanI GGYRCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 1418
BauI CACGAG 1 cut(s) 1505
BbsI GAAGAC 2 cut(s) 252, 336
Bbv12I GWGCWC 1 cut(s) 1418
BbvCI CCTCAGC 1 cut(s) 385
BccI CCATC 1 cut(s) 1228
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 1059
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 2 cut(s) 1188, 1567
BcoDI GTCTC 2 cut(s) 440, 1284
BfaI CTAG 1 cut(s) 1398
BfmI CTRYAG 2 cut(s) 147, 1554
BfoI RGCGCY 2 cut(s) 68, 747
BglII AGATCT 1 cut(s) 1587
Bme1390I CCNGG 3 cut(s) 1059, 1188, 1567
Bme18I GGWCC 3 cut(s) 1098, 1184, 1256
BmgT120I GGNCC 3 cut(s) 1098, 1184, 1256
BmiI GGNNCC 5 cut(s) 66, 87, 903, 1063, 1186
BmrFI CCNGG 3 cut(s) 1059, 1188, 1567
BmrI ACTGGG 1 cut(s) 1180
BmsI GCATC 3 cut(s) 688, 974, 1446
BmuI ACTGGG 1 cut(s) 1180
BpiI GAAGAC 2 cut(s) 252, 336
BpmI CTGGAG 1 cut(s) 936
Bpu10I CCTNAGC 1 cut(s) 385
Bpu14I TTCGAA 1 cut(s) 965
BpuMI CCSGG 2 cut(s) 1188, 1567
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 3 cut(s) 1067, 1079, 1497
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 8 cut(s) 74, 110, 761, 1172, 1194, 1274, 1292, 1566
Bse1I ACTGG 5 cut(s) 450, 599, 919, 1175, 1186
Bse21I CCTNAGG 1 cut(s) 1273
Bse3DI GCAATG 1 cut(s) 1113
BseBI CCWGG 1 cut(s) 1059
BseDI CCNNGG 3 cut(s) 1067, 1079, 1497
BseGI GGATG 5 cut(s) 703, 934, 989, 1220, 1282
BseLI CCNNNNNNNGG 8 cut(s) 74, 110, 761, 1172, 1194, 1274, 1292, 1566
BseMI GCAATG 1 cut(s) 1113
BseMII CTCAG 4 cut(s) 219, 399, 831, 1106
BseNI ACTGG 5 cut(s) 450, 599, 919, 1175, 1186
BseRI GAGGAG 2 cut(s) 792, 1026
BsgI GTGCAG 2 cut(s) 1143, 1162
BshFI GGCC 2 cut(s) 78, 1072
BshNI GGYRCC 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 1418
BsiSI CCGG 4 cut(s) 174, 603, 1188, 1567
BslFI GGGAC 3 cut(s) 486, 1310, 1515
BslI CCNNNNNNNGG 8 cut(s) 74, 110, 761, 1172, 1194, 1274, 1292, 1566
BsmAI GTCTC 2 cut(s) 440, 1284
BsmBI CGTCTC 1 cut(s) 1284
BsmFI GGGAC 3 cut(s) 486, 1310, 1515
BsmI GAATGC 1 cut(s) 1121
BsnI GGCC 2 cut(s) 78, 1072
Bso31I GGTCTC 1 cut(s) 440
Bsp119I TTCGAA 1 cut(s) 965
Bsp1286I GDGCHC 1 cut(s) 1418
Bsp143I GATC 5 cut(s) 316, 883, 944, 1393, 1587
Bsp19I CCATGG 1 cut(s) 1079
BspACI CCGC 4 cut(s) 104, 565, 1010, 1115
BspANI GGCC 2 cut(s) 78, 1072
BspCNI CTCAG 4 cut(s) 218, 398, 832, 1105
BspHI TCATGA 2 cut(s) 612, 1592
BspLI GGNNCC 5 cut(s) 66, 87, 903, 1063, 1186
BspMAI CTGCAG 2 cut(s) 151, 1558
BspPI GGATC 2 cut(s) 952, 1388
BspQI GCTCTTC 1 cut(s) 41
BspT104I TTCGAA 1 cut(s) 965
BspT107I GGYRCC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 1113
BsrI ACTGG 5 cut(s) 450, 599, 919, 1175, 1186
BssECI CCNNGG 3 cut(s) 1067, 1079, 1497
BssMI GATC 5 cut(s) 316, 883, 944, 1393, 1587
BssNAI GTATAC 1 cut(s) 998
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 1 cut(s) 1505
BssT1I CCWWGG 3 cut(s) 1067, 1079, 1497
Bst1107I GTATAC 1 cut(s) 998
Bst2BI CACGAG 1 cut(s) 1505
Bst2UI CCWGG 1 cut(s) 1059
Bst4CI ACNGT 4 cut(s) 584, 778, 1022, 1241
Bst6I CTCTTC 3 cut(s) 41, 864, 1323
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 155
BstBI TTCGAA 1 cut(s) 965
BstC8I GCNNGC 3 cut(s) 1119, 1315, 1545
BstDSI CCRYGG 1 cut(s) 1079
BstF5I GGATG 5 cut(s) 703, 934, 989, 1220, 1282
BstH2I RGCGCY 2 cut(s) 68, 747
BstHHI GCGC 2 cut(s) 67, 746
BstKTI GATC 5 cut(s) 319, 886, 947, 1396, 1590
BstMAI GTCTC 2 cut(s) 440, 1284
BstMBI GATC 5 cut(s) 316, 883, 944, 1393, 1587
BstNI CCWGG 1 cut(s) 1059
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 3 cut(s) 1057, 1186, 1565
BstSFI CTRYAG 2 cut(s) 147, 1554
BstV2I GAAGAC 2 cut(s) 252, 336
BstX2I RGATCY 1 cut(s) 1587
BstXI CCANNNNNNTGG 3 cut(s) 55, 941, 1170
BstYI RGATCY 1 cut(s) 1587
BstZ17I GTATAC 1 cut(s) 998
Bsu36I CCTNAGG 1 cut(s) 1273
BsuRI GGCC 2 cut(s) 78, 1072
BtgI CCRYGG 1 cut(s) 1079
BtsCI GGATG 5 cut(s) 703, 934, 989, 1220, 1282
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 5 cut(s) 285, 774, 1100, 1179, 1237
Cac8I GCNNGC 3 cut(s) 1119, 1315, 1545
CaiI CAGNNNCTG 1 cut(s) 155
CciI TCATGA 2 cut(s) 612, 1592
CfoI GCGC 2 cut(s) 67, 746
Cfr13I GGNCC 3 cut(s) 1098, 1184, 1256
Csp6I GTAC 4 cut(s) 878, 1046, 1202, 1245
CviQI GTAC 4 cut(s) 878, 1046, 1202, 1245
DinI GGCGCC 1 cut(s) 66
DpnI GATC 5 cut(s) 318, 885, 946, 1395, 1589
DpnII GATC 5 cut(s) 316, 883, 944, 1393, 1587
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 3 cut(s) 41, 864, 1323
EarI CTCTTC 3 cut(s) 41, 864, 1323
EciI GGCGGA 1 cut(s) 1104
Ecl136II GAGCTC 1 cut(s) 1416
Eco130I CCWWGG 3 cut(s) 1067, 1079, 1497
Eco24I GRGCYC 1 cut(s) 1418
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 3 cut(s) 1098, 1184, 1256
Eco53kI GAGCTC 1 cut(s) 1416
Eco57I CTGAAG 4 cut(s) 264, 387, 494, 845
Eco81I CCTNAGG 1 cut(s) 1273
EcoICRI GAGCTC 1 cut(s) 1416
EcoRI GAATTC 3 cut(s) 281, 894, 967
EcoRII CCWGG 1 cut(s) 1057
EcoT14I CCWWGG 3 cut(s) 1067, 1079, 1497
EcoT38I GRGCYC 1 cut(s) 1418
EgeI GGCGCC 1 cut(s) 66
EheI GGCGCC 1 cut(s) 66
ErhI CCWWGG 3 cut(s) 1067, 1079, 1497
Esp3I CGTCTC 1 cut(s) 1284
FaqI GGGAC 3 cut(s) 486, 1310, 1515
FauI CCCGC 2 cut(s) 111, 572
FbaI TGATCA 1 cut(s) 883
FblI GTMKAC 1 cut(s) 997
FokI GGATG 5 cut(s) 710, 941, 996, 1207, 1269
FriOI GRGCYC 1 cut(s) 1418
FspBI CTAG 1 cut(s) 1398
GlaI GCGC 2 cut(s) 66, 745
GsuI CTGGAG 1 cut(s) 936
HaeII RGCGCY 2 cut(s) 68, 747
HaeIII GGCC 2 cut(s) 78, 1072
HapII CCGG 4 cut(s) 174, 603, 1188, 1567
HhaI GCGC 2 cut(s) 67, 746
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 2 cut(s) 65, 744
HinP1I GCGC 2 cut(s) 65, 744
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 2 cut(s) 212, 1027
HpaII CCGG 4 cut(s) 174, 603, 1188, 1567
HphI GGTGA 1 cut(s) 1080
Hpy166II GTNNAC 4 cut(s) 133, 998, 1015, 1098
Hpy188III TCNNGA 4 cut(s) 357, 613, 1441, 1593
Hpy8I GTNNAC 4 cut(s) 133, 998, 1015, 1098
Hpy99I CGWCG 2 cut(s) 358, 645
HpyAV CCTTC 1 cut(s) 915
HpyCH4III ACNGT 4 cut(s) 584, 778, 1022, 1241
HpyCH4IV ACGT 2 cut(s) 552, 1379
HpySE526I ACGT 2 cut(s) 552, 1379
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 2 cut(s) 65, 744
KasI GGCGCC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 5 cut(s) 316, 883, 944, 1393, 1587
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 3 cut(s) 113, 632, 1067
LweI GCATC 3 cut(s) 688, 974, 1446
MaeI CTAG 1 cut(s) 1398
MaeII ACGT 2 cut(s) 552, 1379
MaeIII GTNAC 4 cut(s) 533, 680, 955, 1569
MalI GATC 5 cut(s) 318, 885, 946, 1395, 1589
MboI GATC 5 cut(s) 316, 883, 944, 1393, 1587
MflI RGATCY 1 cut(s) 1587
MhlI GDGCHC 1 cut(s) 1418
MluCI AATT 8 cut(s) 281, 320, 360, 894, 967, 1210, 1261, 1456
Mly113I GGCGCC 1 cut(s) 65
MlyI GAGTC 4 cut(s) 116, 128, 463, 1340
MmeI TCCRAC 5 cut(s) 113, 144, 368, 675, 779
MseI TTAA 2 cut(s) 689, 1382
MslI CAYNNNNRTG 2 cut(s) 689, 1093
MspA1I CMGCKG 3 cut(s) 152, 389, 1010
MspI CCGG 4 cut(s) 174, 603, 1188, 1567
MspR9I CCNGG 3 cut(s) 1059, 1188, 1567
Mva1269I GAATGC 1 cut(s) 1121
MvaI CCWGG 1 cut(s) 1059
NarI GGCGCC 1 cut(s) 65
NciI CCSGG 2 cut(s) 1188, 1567
NcoI CCATGG 1 cut(s) 1079
NdeII GATC 5 cut(s) 316, 883, 944, 1393, 1587
NlaIV GGNNCC 5 cut(s) 66, 87, 903, 1063, 1186
NmuCI GTSAC 2 cut(s) 680, 1569
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 965
OliI CACNNNNGTG 1 cut(s) 1093
PagI TCATGA 2 cut(s) 612, 1592
PciI ACATGT 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 41
PctI GAATGC 1 cut(s) 1121
PfeI GAWTC 6 cut(s) 370, 609, 797, 812, 1369, 1493
PleI GAGTC 4 cut(s) 116, 128, 463, 1339
PluTI GGCGCC 1 cut(s) 68
PpsI GAGTC 4 cut(s) 116, 128, 463, 1339
PscI ACATGT 1 cut(s) 684
Psp124BI GAGCTC 1 cut(s) 1418
Psp1406I AACGTT 1 cut(s) 1379
Psp6I CCWGG 1 cut(s) 1057
PspGI CCWGG 1 cut(s) 1057
PspN4I GGNNCC 5 cut(s) 66, 87, 903, 1063, 1186
PspPI GGNCC 3 cut(s) 1098, 1184, 1256
PstI CTGCAG 2 cut(s) 151, 1558
PstNI CAGNNNCTG 1 cut(s) 155
PsuI RGATCY 1 cut(s) 1587
PvuII CAGCTG 2 cut(s) 152, 389
RsaI GTAC 4 cut(s) 879, 1047, 1203, 1246
RsaNI GTAC 4 cut(s) 878, 1046, 1202, 1245
RseI CAYNNNNRTG 2 cut(s) 689, 1093
SacI GAGCTC 1 cut(s) 1418
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 689, 1382
Sau3AI GATC 5 cut(s) 316, 883, 944, 1393, 1587
Sau96I GGNCC 3 cut(s) 1098, 1184, 1256
SchI GAGTC 4 cut(s) 116, 128, 463, 1340
ScrFI CCNGG 3 cut(s) 1059, 1188, 1567
SduI GDGCHC 1 cut(s) 1418
SfaNI GCATC 3 cut(s) 688, 974, 1446
SfcI CTRYAG 2 cut(s) 147, 1554
SfoI GGCGCC 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 965
SinI GGWCC 3 cut(s) 1098, 1184, 1256
SmiMI CAYNNNNRTG 2 cut(s) 689, 1093
Sse9I AATT 8 cut(s) 281, 320, 360, 894, 967, 1210, 1261, 1456
SsiI CCGC 4 cut(s) 104, 565, 1010, 1115
SspDI GGCGCC 1 cut(s) 64
SspMI CTAG 1 cut(s) 1398
SstI GAGCTC 1 cut(s) 1418
StyD4I CCNGG 3 cut(s) 1057, 1186, 1565
StyI CCWWGG 3 cut(s) 1067, 1079, 1497
TaaI ACNGT 4 cut(s) 584, 778, 1022, 1241
TaiI ACGT 2 cut(s) 555, 1382
TaqI TCGA 1 cut(s) 965
TasI AATT 8 cut(s) 281, 320, 360, 894, 967, 1210, 1261, 1456
TfiI GAWTC 6 cut(s) 370, 609, 797, 812, 1369, 1493
Tru1I TTAA 2 cut(s) 689, 1382
Tru9I TTAA 2 cut(s) 689, 1382
TscAI CASTG 5 cut(s) 292, 781, 1100, 1186, 1244
TseFI GTSAC 2 cut(s) 680, 1569
Tsp45I GTSAC 2 cut(s) 680, 1569
TspDTI ATGAA 5 cut(s) 27, 651, 863, 960, 1461
TspGWI ACGGA 1 cut(s) 1571
TspRI CASTG 5 cut(s) 292, 781, 1100, 1186, 1244
VpaK11BI GGWCC 3 cut(s) 1098, 1184, 1256
XapI RAATTY 5 cut(s) 281, 894, 967, 1261, 1456
XceI RCATGY 1 cut(s) 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 1065
XmiI GTMKAC 1 cut(s) 997
XspI CTAG 1 cut(s) 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.