Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003750

Physical Location & Seq

Genomic Coordinates Reverse (-)

5018920 .. 5021950

Gene Structure 3031 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1071400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1572 bp

ATGTCGTCGGAGGGTGAAGGAGGAGGAGGCCACACGATAAAGCTGTTCGGAAAGACTATACCTTTGGCTATGGCGCCACTGAACCGTGAGTCTTCTTCCGCCATTGACGAACCTTGTGGATCGGTGGACCATGATGTCGCCGCCACTGCTTGTGGCGGGGGTGGGGGCGGTGCTGAAGACTGTTCTGAAACCAGGCAGAAGCTTTCCTCCTCTTCTTCAGCTGCTACCAATTCAGGGGAAGATAACTTGGAGAGGGAAGGAGATGCACAACAAGAGGAAACAAAATCAGGAAAAGAACTAGCAGCTGATAAACAGGATGCTGATACCTCAGATCTAATCACAGAAGACCTAAAAGCTCCTACAACATCATCCGGAATTAGCGAGAATCCTAAAACTCCTTCAGCTGATAGAGAAACTTCATGTAAGAATGGAGACCAGAGCGAGACTAGTAGCTCACAAGATAAGACTCTGAAGAAGCCAGACAAGATACTTCCATGCCCTCGATGTAATAGTATGGACACCAAGTTCTGTTACTACAACAACTACAATGTTAACCAGCCTCGCCATTTCTGCAAGAACTGCCAGAGATATTGGACTGCTGGAGGAATCATGAGGAATGTGCCGGTGGGTGCTGGTCGTCGCAAGAATAAGAACTCTTCAGCTTCACAGTTTCATCACGTATTAATGTCAGATGCTTTCCAAGCAGCTCATGCTAGCGCTGCAAATGGTGTGCACAATGCTACTTTGGGAAGCAACGGCACTGTCCTTAACTTTGGTTCAGATTCTCCTCTTTGTGAATCAATGGCTTCTGTGTTGAACCTTGCAGAGAAAAACCAAAATTGTATGCAAAATGGATTTCGTGGACCCGAACAAAGACTCCTAGTTTCTCCCACTGCTGGAGATAATGGGGACGACCGCTCAAGTGGATCATCCATTACAGCTTCAAATTCGACAGAGAGAGGAGGCAAAGCTAATTTACAAGAGCCTTCAGCTCAAAAGTGTCAAAGCTTCCCTCCTCAAGTACCATGCTTTCCGGGTTCCCCTTGGCCTTATCCATGCAACTCAGCTCAGTGGGCCTCTGCAATGCCTCCACCCGGCTTCTGCCATTCAGGCTTTCCGATGGCATTCTACCCTGCACCACCATATTGGGGCTGCACTGTGCCGGGCTCTTGGAATGTACCATGTCTCTCACCGTCCTCTACTCTTAGCCATTGCACTACAAGCTCCGGTCCAAATTCCCCAACCTTAGGGAAACATTCAAGAGACGAGGACATTCCTAATCCATCCAGCTCTGAGAATGAAGAGCCCGCAAAAGATAACAGTTCCGAAAGATGTGTTTGGATCCCAAAAACATTAAGGATCGATGATTCTAATGAGGCTGCAAGAAGCTCTGCATGGGCAACGCTTGGCATTAGGAATGAAAGGGGAAATTGTGCTAACGAGGGAGGAGGTCACTTCAAGGCATTTCAATCAAAGGGAGATGAGAAAACTCCCGCAGTTGAAGCTTCTCGAGCGTTGCAATCGAATCCTGCAGCGTTTTCCCGGTCACTCAACTTCCATGAGAGAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0048519 GO:0048577 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048587 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-Dof

Protein Analysis

524

Amino Acids

55.49

Weight (kDa)

5.71

Isoelectric Point (pI)

53.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_Dof	PF02701	162 - 218	1.5e-32	Dof domain, zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G39660 AT5G39660 AT5G39660
fragaria_vesca	FvH4_4g31210 FvH4_6g48270 FvH4_6g48270
malus_domestica	MD00G1125100.v1.1 MD13G1070600.v1.1 MD16G1071400.v1.1
prunus_persica	Prupe.1G276000_v2.0.a1 Prupe.3G266200_v2.0.a1
pyrus_communis	pycom111g04390 pycom13g06340 pycom16g06180 pycom17g04800
rosa_chinensis	RchiOBHm_Chr2g0167771 RchiOBHm_Chr4g0439611
rosa_laevigata	RLG00000006255 RLG00000021752
rosa_multiflora	Rmu_sc0002549.1_g000020
rosa_roxburghii	Rroxscaffold_2G00083270 Rroxscaffold_5G00380530
rosa_rugosa	Rorug02G0532000 Rorug04G0320000
rosa_samantha	Rh2AG600900 Rh2BG611600 Rh2CG581800 Rh2DG624000 Rh4AG371200 Rh4BG382900 Rh4CG397500 Rh4DG377700
rosa_wichuraiana	Rw2G049900 Rw4G031640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	134
AccB1I	GGYRCC	1 cut(s)	73
AccBSI	CCGCTC	1 cut(s)	918
AccIII	TCCGGA	1 cut(s)	371
AciI	CCGC	7 cut(s)	99, 141, 156, 168, 916, 1308, 1494
AclWI	GGATC	5 cut(s)	127, 934, 1336, 1349, 1367
AcsI	RAATTY	2 cut(s)	946, 1234
AcuI	CTGAAG	6 cut(s)	195, 201, 384, 491, 642, 972
AcyI	GRCGYC	1 cut(s)	74
AfaI	GTAC	2 cut(s)	1023, 1179
AfeI	AGCGCT	1 cut(s)	718
AfiI	CCNNNNNNNGG	5 cut(s)	234, 896, 1094, 1148, 1247
AgsI	TTSAA	6 cut(s)	817, 945, 1260, 1459, 1469, 1502
AhlI	ACTAGT	1 cut(s)	446
AjnI	CCWGG	1 cut(s)	191
AloI	GAACNNNNNNTCC	4 cut(s)	509, 541, 861, 893
Alw21I	GWGCWC	1 cut(s)	735
Alw26I	GTCTC	4 cut(s)	426, 437, 1190, 1257
Alw44I	GTGCAC	1 cut(s)	731
AlwI	GGATC	5 cut(s)	127, 934, 1336, 1349, 1367
Ama87I	CYCGRG	1 cut(s)	1509
Aor13HI	TCCGGA	1 cut(s)	371
Aor51HI	AGCGCT	1 cut(s)	718
AoxI	GGCC	3 cut(s)	28, 1046, 1074
ApaLI	GTGCAC	1 cut(s)	731
ApeKI	GCWGC	7 cut(s)	221, 302, 704, 719, 1152, 1379, 1532
ApoI	RAATTY	2 cut(s)	946, 1234
ArsI	GACNNNNNNTTYG	2 cut(s)	46, 78
AseI	ATTAAT	1 cut(s)	683
AspLEI	GCGC	2 cut(s)	76, 719
AspS9I	GGNCC	4 cut(s)	127, 863, 1074, 1229
AsuC2I	CCSGG	4 cut(s)	1035, 1095, 1164, 1543
AsuHPI	GGTGA	2 cut(s)	26, 1182
AsuNHI	GCTAGC	1 cut(s)	713
AvaI	CYCGRG	1 cut(s)	1509
AvaII	GGWCC	3 cut(s)	127, 863, 1229
AxyI	CCTNAGG	1 cut(s)	1246
BaeGI	GKGCMC	1 cut(s)	735
BamHI	GGATCC	1 cut(s)	1341
BanI	GGYRCC	1 cut(s)	73
BanII	GRGCYC	2 cut(s)	1169, 1308
BbsI	GAAGAC	3 cut(s)	84, 183, 351
Bbv12I	GWGCWC	1 cut(s)	735
BbvI	GCAGC	7 cut(s)	208, 314, 706, 716, 1139, 1366, 1544
BccI	CCATC	2 cut(s)	1114, 1291
BceAI	ACGGC	1 cut(s)	772
BciT130I	CCWGG	1 cut(s)	193
BcnI	CCSGG	4 cut(s)	1035, 1095, 1164, 1543
BcoDI	GTCTC	4 cut(s)	426, 437, 1190, 1257
BcuI	ACTAGT	1 cut(s)	446
BfaI	CTAG	4 cut(s)	299, 447, 714, 881
BfmI	CTRYAG	1 cut(s)	1530
BfoI	RGCGCY	2 cut(s)	77, 720
BglI	GCCNNNNNGGC	1 cut(s)	1110
BglII	AGATCT	1 cut(s)	331
BisI	GCNGC	8 cut(s)	141, 222, 303, 705, 720, 1153, 1380, 1533
BlsI	GCNGC	8 cut(s)	142, 223, 304, 706, 721, 1154, 1381, 1534
Bme1390I	CCNGG	5 cut(s)	193, 1035, 1095, 1164, 1543
Bme18I	GGWCC	3 cut(s)	127, 863, 1229
BmeT110I	CYCGRG	1 cut(s)	1509
BmgT120I	GGNCC	4 cut(s)	127, 863, 1074, 1229
BmiI	GGNNCC	4 cut(s)	75, 865, 1039, 1343
BmrFI	CCNGG	5 cut(s)	193, 1035, 1095, 1164, 1543
BmsI	GCATC	3 cut(s)	253, 307, 682
BmtI	GCTAGC	1 cut(s)	717
BpiI	GAAGAC	3 cut(s)	84, 183, 351
BpmI	CTGGAG	2 cut(s)	621, 918
BpuEI	CTTGAG	2 cut(s)	904, 1002
BpuMI	CCSGG	4 cut(s)	1035, 1095, 1164, 1543
Bsa29I	ATCGAT	1 cut(s)	1362
BsaAI	YACGTR	1 cut(s)	679
BsaHI	GRCGYC	1 cut(s)	74
BsaI	GGTCTC	1 cut(s)	426
BsaJI	CCNNGG	1 cut(s)	1043
BsaWI	WCCGGW	2 cut(s)	371, 1226
BsaXI	ACNNNNNCTCC	2 cut(s)	861, 891
Bsc4I	CCNNNNNNNGG	5 cut(s)	234, 896, 1094, 1148, 1247
Bse118I	RCCGGY	1 cut(s)	622
Bse21I	CCTNAGG	1 cut(s)	1246
Bse3DI	GCAATG	2 cut(s)	1089, 1210
BseAI	TCCGGA	1 cut(s)	371
BseBI	CCWGG	1 cut(s)	193
BseCI	ATCGAT	1 cut(s)	1362
BseDI	CCNNGG	1 cut(s)	1043
BseGI	GGATG	4 cut(s)	322, 368, 929, 1283
BseLI	CCNNNNNNNGG	5 cut(s)	234, 896, 1094, 1148, 1247
BseMI	GCAATG	2 cut(s)	1089, 1210
BseMII	CTCAG	4 cut(s)	342, 1077, 1082, 1284
BseRI	GAGGAG	7 cut(s)	36, 39, 199, 777, 975, 1005, 1461
BseSI	GKGCMC	1 cut(s)	735
BseXI	GCAGC	7 cut(s)	208, 314, 706, 716, 1139, 1366, 1544
BsgI	GTGCAG	2 cut(s)	1119, 1138
Bsh1285I	CGRYCG	1 cut(s)	916
BshFI	GGCC	3 cut(s)	30, 1048, 1076
BshNI	GGYRCC	1 cut(s)	73
BshVI	ATCGAT	1 cut(s)	1362
BsiEI	CGRYCG	1 cut(s)	916
BsiHKAI	GWGCWC	1 cut(s)	735
BsiHKCI	CYCGRG	1 cut(s)	1509
BsiSI	CCGG	7 cut(s)	372, 623, 1034, 1095, 1163, 1227, 1543
BslFI	GGGAC	1 cut(s)	923
BslI	CCNNNNNNNGG	5 cut(s)	234, 896, 1094, 1148, 1247
BsmAI	GTCTC	4 cut(s)	426, 437, 1190, 1257
BsmBI	CGTCTC	1 cut(s)	1257
BsmFI	GGGAC	1 cut(s)	923
BsmI	GAATGC	1 cut(s)	1124
BsnI	GGCC	3 cut(s)	30, 1048, 1076
Bso31I	GGTCTC	1 cut(s)	426
BsoBI	CYCGRG	1 cut(s)	1509
Bsp1286I	GDGCHC	3 cut(s)	735, 1169, 1308
Bsp13I	TCCGGA	1 cut(s)	371
Bsp143I	GATC	5 cut(s)	119, 331, 926, 1341, 1359
BspACI	CCGC	7 cut(s)	99, 141, 156, 168, 916, 1308, 1494
BspANI	GGCC	3 cut(s)	30, 1048, 1076
BspCNI	CTCAG	4 cut(s)	341, 1076, 1081, 1285
BspDI	ATCGAT	1 cut(s)	1362
BspEI	TCCGGA	1 cut(s)	371
BspHI	TCATGA	1 cut(s)	609
BspLI	GGNNCC	4 cut(s)	75, 865, 1039, 1343
BspMAI	CTGCAG	1 cut(s)	1534
BspOI	GCTAGC	1 cut(s)	717
BspPI	GGATC	5 cut(s)	127, 934, 1336, 1349, 1367
BspQI	GCTCTTC	1 cut(s)	1296
BspT107I	GGYRCC	1 cut(s)	73
BspTNI	GGTCTC	1 cut(s)	426
BsrBI	CCGCTC	1 cut(s)	918
BsrDI	GCAATG	2 cut(s)	1089, 1210
BsrFI	RCCGGY	1 cut(s)	622
BssAI	RCCGGY	1 cut(s)	622
BssECI	CCNNGG	1 cut(s)	1043
BssMI	GATC	5 cut(s)	119, 331, 926, 1341, 1359
BssNI	GRCGYC	1 cut(s)	74
BssT1I	CCWWGG	1 cut(s)	1043
Bst2UI	CCWGG	1 cut(s)	193
Bst4CI	ACNGT	7 cut(s)	86, 182, 669, 763, 1159, 1194, 1322
Bst6I	CTCTTC	3 cut(s)	217, 661, 1296
BstACI	GRCGYC	1 cut(s)	74
BstAPI	GCANNNNNTGC	2 cut(s)	579, 710
BstBAI	YACGTR	1 cut(s)	679
BstC8I	GCNNGC	2 cut(s)	715, 1308
BstDEI	CTNAG	6 cut(s)	328, 1063, 1068, 1205, 1246, 1293
BstF5I	GGATG	4 cut(s)	322, 368, 929, 1283
BstH2I	RGCGCY	2 cut(s)	77, 720
BstHHI	GCGC	2 cut(s)	76, 719
BstKTI	GATC	5 cut(s)	122, 334, 929, 1344, 1362
BstMAI	GTCTC	4 cut(s)	426, 437, 1190, 1257
BstMBI	GATC	5 cut(s)	119, 331, 926, 1341, 1359
BstMCI	CGRYCG	1 cut(s)	916
BstNI	CCWGG	1 cut(s)	193
BstSCI	CCNGG	5 cut(s)	191, 1033, 1093, 1162, 1541
BstSFI	CTRYAG	1 cut(s)	1530
BstSLI	GKGCMC	1 cut(s)	735
BstV1I	GCAGC	7 cut(s)	208, 314, 706, 716, 1139, 1366, 1544
BstV2I	GAAGAC	3 cut(s)	84, 183, 351
BstX2I	RGATCY	2 cut(s)	331, 1341
BstXI	CCANNNNNNTGG	1 cut(s)	1146
BstYI	RGATCY	2 cut(s)	331, 1341
Bsu15I	ATCGAT	1 cut(s)	1362
Bsu36I	CCTNAGG	1 cut(s)	1246
BsuRI	GGCC	3 cut(s)	30, 1048, 1076
BsuTUI	ATCGAT	1 cut(s)	1362
BtsCI	GGATG	4 cut(s)	322, 368, 929, 1283
BtsI	GCAGTG	2 cut(s)	144, 891
BtsIMutI	CAGTG	6 cut(s)	77, 144, 759, 891, 1076, 1155
Cac8I	GCNNGC	2 cut(s)	715, 1308
CciI	TCATGA	1 cut(s)	609
CfoI	GCGC	2 cut(s)	76, 719
Cfr10I	RCCGGY	1 cut(s)	622
Cfr13I	GGNCC	4 cut(s)	127, 863, 1074, 1229
ClaI	ATCGAT	1 cut(s)	1362
Csp6I	GTAC	2 cut(s)	1022, 1178
CviQI	GTAC	2 cut(s)	1022, 1178
DdeI	CTNAG	6 cut(s)	328, 1063, 1068, 1205, 1246, 1293
DinI	GGCGCC	1 cut(s)	75
DpnI	GATC	5 cut(s)	121, 333, 928, 1343, 1361
DpnII	GATC	5 cut(s)	119, 331, 926, 1341, 1359
DrdI	GACNNNNNNGTC	1 cut(s)	134
DseDI	GACNNNNNNGTC	1 cut(s)	134
Eam1104I	CTCTTC	3 cut(s)	217, 661, 1296
EarI	CTCTTC	3 cut(s)	217, 661, 1296
EciI	GGCGGA	1 cut(s)	88
Eco130I	CCWWGG	1 cut(s)	1043
Eco24I	GRGCYC	2 cut(s)	1169, 1308
Eco31I	GGTCTC	1 cut(s)	426
Eco47I	GGWCC	3 cut(s)	127, 863, 1229
Eco47III	AGCGCT	1 cut(s)	718
Eco57I	CTGAAG	6 cut(s)	195, 201, 384, 491, 642, 972
Eco81I	CCTNAGG	1 cut(s)	1246
Eco88I	CYCGRG	1 cut(s)	1509
EcoRII	CCWGG	1 cut(s)	191
EcoT14I	CCWWGG	1 cut(s)	1043
EcoT38I	GRGCYC	2 cut(s)	1169, 1308
EgeI	GGCGCC	1 cut(s)	75
EheI	GGCGCC	1 cut(s)	75
ErhI	CCWWGG	1 cut(s)	1043
Esp3I	CGTCTC	1 cut(s)	1257
FaqI	GGGAC	1 cut(s)	923
FauI	CCCGC	3 cut(s)	149, 1315, 1501
Fnu4HI	GCNGC	8 cut(s)	141, 222, 303, 705, 720, 1153, 1380, 1533
FokI	GGATG	4 cut(s)	329, 355, 916, 1270
FriOI	GRGCYC	2 cut(s)	1169, 1308
Fsp4HI	GCNGC	8 cut(s)	141, 222, 303, 705, 720, 1153, 1380, 1533
FspBI	CTAG	4 cut(s)	299, 447, 714, 881
GlaI	GCGC	2 cut(s)	75, 718
GluI	GCNGC	8 cut(s)	141, 222, 303, 705, 720, 1153, 1380, 1533
GsuI	CTGGAG	2 cut(s)	621, 918
HaeII	RGCGCY	2 cut(s)	77, 720
HaeIII	GGCC	3 cut(s)	30, 1048, 1076
HapII	CCGG	7 cut(s)	372, 623, 1034, 1095, 1163, 1227, 1543
HhaI	GCGC	2 cut(s)	76, 719
Hin1I	GRCGYC	1 cut(s)	74
Hin6I	GCGC	2 cut(s)	74, 717
HinP1I	GCGC	2 cut(s)	74, 717
HincII	GTYRAC	1 cut(s)	553
HindII	GTYRAC	1 cut(s)	553
HindIII	AAGCTT	3 cut(s)	200, 1006, 1503
HinfI	GANTC	9 cut(s)	89, 385, 466, 606, 782, 797, 876, 1367, 1525
HpaI	GTTAAC	1 cut(s)	553
HpaII	CCGG	7 cut(s)	372, 623, 1034, 1095, 1163, 1227, 1543
HphI	GGTGA	2 cut(s)	26, 1182
Hpy166II	GTNNAC	4 cut(s)	127, 553, 733, 863
Hpy188III	TCNNGA	5 cut(s)	288, 372, 610, 1260, 1509
Hpy8I	GTNNAC	4 cut(s)	127, 553, 733, 863
Hpy99I	CGWCG	2 cut(s)	10, 642
HpyAV	CCTTC	4 cut(s)	11, 251, 408, 996
HpyCH4III	ACNGT	7 cut(s)	86, 182, 669, 763, 1159, 1194, 1322
HpyCH4IV	ACGT	1 cut(s)	678
HpyF3I	CTNAG	6 cut(s)	328, 1063, 1068, 1205, 1246, 1293
HpySE526I	ACGT	1 cut(s)	678
Hsp92I	GRCGYC	1 cut(s)	74
HspAI	GCGC	2 cut(s)	74, 717
KasI	GGCGCC	1 cut(s)	73
Kpn2I	TCCGGA	1 cut(s)	371
KspAI	GTTAAC	1 cut(s)	553
Kzo9I	GATC	5 cut(s)	119, 331, 926, 1341, 1359
LguI	GCTCTTC	1 cut(s)	1296
LmnI	GCTCC	2 cut(s)	361, 1229
Lsp1109I	GCAGC	7 cut(s)	208, 314, 706, 716, 1139, 1366, 1544
LweI	GCATC	3 cut(s)	253, 307, 682
MaeI	CTAG	4 cut(s)	299, 447, 714, 881
MaeII	ACGT	1 cut(s)	678
MaeIII	GTNAC	3 cut(s)	530, 1451, 1545
MalI	GATC	5 cut(s)	121, 333, 928, 1343, 1361
MbiI	CCGCTC	1 cut(s)	918
MboI	GATC	5 cut(s)	119, 331, 926, 1341, 1359
MflI	RGATCY	2 cut(s)	331, 1341
MhlI	GDGCHC	3 cut(s)	735, 1169, 1308
MluCI	AATT	7 cut(s)	229, 375, 838, 946, 973, 1234, 1429
Mly113I	GGCGCC	1 cut(s)	74
MlyI	GAGTC	3 cut(s)	98, 460, 870
MroI	TCCGGA	1 cut(s)	371
MseI	TTAA	4 cut(s)	552, 683, 768, 1355
MspA1I	CMGCKG	3 cut(s)	221, 305, 404
MspI	CCGG	7 cut(s)	372, 623, 1034, 1095, 1163, 1227, 1543
MspR9I	CCNGG	5 cut(s)	193, 1035, 1095, 1164, 1543
Mva1269I	GAATGC	1 cut(s)	1124
MvaI	CCWGG	1 cut(s)	193
NarI	GGCGCC	1 cut(s)	74
NciI	CCSGG	4 cut(s)	1035, 1095, 1164, 1543
NdeII	GATC	5 cut(s)	119, 331, 926, 1341, 1359
NheI	GCTAGC	1 cut(s)	713
NlaIV	GGNNCC	4 cut(s)	75, 865, 1039, 1343
NmuCI	GTSAC	2 cut(s)	1451, 1545
PaeR7I	CTCGAG	1 cut(s)	1509
PagI	TCATGA	1 cut(s)	609
PciSI	GCTCTTC	1 cut(s)	1296
PctI	GAATGC	1 cut(s)	1124
PfeI	GAWTC	6 cut(s)	385, 606, 782, 797, 1367, 1525
PkrI	GCNGC	8 cut(s)	142, 223, 304, 706, 721, 1154, 1381, 1534
PleI	GAGTC	3 cut(s)	97, 460, 870
PluTI	GGCGCC	1 cut(s)	77
PpsI	GAGTC	3 cut(s)	97, 460, 870
Ppu21I	YACGTR	1 cut(s)	679
PshBI	ATTAAT	1 cut(s)	683
Psp6I	CCWGG	1 cut(s)	191
PspGI	CCWGG	1 cut(s)	191
PspN4I	GGNNCC	4 cut(s)	75, 865, 1039, 1343
PspPI	GGNCC	4 cut(s)	127, 863, 1074, 1229
PstI	CTGCAG	1 cut(s)	1534
PsuI	RGATCY	2 cut(s)	331, 1341
PvuII	CAGCTG	3 cut(s)	221, 305, 404
RsaI	GTAC	2 cut(s)	1023, 1179
RsaNI	GTAC	2 cut(s)	1022, 1178
SapI	GCTCTTC	1 cut(s)	1296
SaqAI	TTAA	4 cut(s)	552, 683, 768, 1355
SatI	GCNGC	8 cut(s)	141, 222, 303, 705, 720, 1153, 1380, 1533
Sau3AI	GATC	5 cut(s)	119, 331, 926, 1341, 1359
Sau96I	GGNCC	4 cut(s)	127, 863, 1074, 1229
SchI	GAGTC	3 cut(s)	98, 460, 870
ScrFI	CCNGG	5 cut(s)	193, 1035, 1095, 1164, 1543
SduI	GDGCHC	3 cut(s)	735, 1169, 1308
SfaNI	GCATC	3 cut(s)	253, 307, 682
SfcI	CTRYAG	1 cut(s)	1530
SfoI	GGCGCC	1 cut(s)	75
Sfr274I	CTCGAG	1 cut(s)	1509
SinI	GGWCC	3 cut(s)	127, 863, 1229
SlaI	CTCGAG	1 cut(s)	1509
SmlI	CTYRAG	3 cut(s)	919, 1017, 1509
SmoI	CTYRAG	3 cut(s)	919, 1017, 1509
SpeI	ACTAGT	1 cut(s)	446
Sse9I	AATT	7 cut(s)	229, 375, 838, 946, 973, 1234, 1429
SsiI	CCGC	7 cut(s)	99, 141, 156, 168, 916, 1308, 1494
SspDI	GGCGCC	1 cut(s)	73
SspMI	CTAG	4 cut(s)	299, 447, 714, 881
StyD4I	CCNGG	5 cut(s)	191, 1033, 1093, 1162, 1541
StyI	CCWWGG	1 cut(s)	1043
TaaI	ACNGT	7 cut(s)	86, 182, 669, 763, 1159, 1194, 1322
TaiI	ACGT	1 cut(s)	681
TaqI	TCGA	5 cut(s)	502, 950, 1362, 1510, 1523
TasI	AATT	7 cut(s)	229, 375, 838, 946, 973, 1234, 1429
TauI	GCSGC	1 cut(s)	143
TfiI	GAWTC	6 cut(s)	385, 606, 782, 797, 1367, 1525
Tru1I	TTAA	4 cut(s)	552, 683, 768, 1355
Tru9I	TTAA	4 cut(s)	552, 683, 768, 1355
TscAI	CASTG	6 cut(s)	84, 151, 766, 898, 1076, 1162
TseFI	GTSAC	2 cut(s)	1451, 1545
TseI	GCWGC	7 cut(s)	221, 302, 704, 719, 1152, 1379, 1532
Tsp45I	GTSAC	2 cut(s)	1451, 1545
TspDTI	ATGAA	4 cut(s)	408, 662, 1314, 1434
TspRI	CASTG	6 cut(s)	84, 151, 766, 898, 1076, 1162
VneI	GTGCAC	1 cut(s)	731
VpaK11BI	GGWCC	3 cut(s)	127, 863, 1229
VspI	ATTAAT	1 cut(s)	683
XapI	RAATTY	2 cut(s)	946, 1234
XhoI	CTCGAG	1 cut(s)	1509
XspI	CTAG	4 cut(s)	299, 447, 714, 881

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.