Rw4G031640

Cyclic dof factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003750
Physical Location & Seq
Reverse (-)
58776162 .. 58779370
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031640.1

Sequence Viewer

Length: 1581 bp
ATGTCTGAGAATGAAATCAGCCCCACCATAAAGCTCTTCGGAAAGACCATACCTTTGGCTTCGGCGCCACACAACGGCCATGAGGTTCCGACCAATGATGACCCGCCATGTGGAGCTTCCGACTCTTCTGTTGACTCTAAGATTGCTGCTGCTGCTTCCGGTGGTGGTGCTGTTGAAGAAGAAGATTGCTCAGTCAAGCTTCCTTGTTGTTCAGAAACTACTTCGCCTGAAGACCAAGAGAACAAGGAAACAATATCAGGCAAAGAATTCACTGCTGACAAAGAGGAAGATCATCCCTTAGATCAATTCACAGAAGACTTGAAACCTCCAACAACATCGTCGGGAATTAGTGAGAATCCCAAAACTCCCTCAGCTGATAGAGAAACTTCAGTATCTCTGAAATCTTCTAAGAATGGAGAACAGAGTGATGAGACCAGTTGCTCACAAGACAAGACTCTGAAGAAGCCAGACAAGATACTTCCATGTCCCCGATGTAATAGCATGGACACCAAGTTCTGTTACTACAACAACTACAACGTCAATCAGCCCCGCCATTTTTGCAAGAACTGTCAAAGATACTGGACTGCCGGAGGAATCATGAGGAATGTTCCTGTGGGAGCTGGTCGTCGGAAGAACAAGAACGCTTCATCAGCTTCACACTATCGTCACATGTTAATGTCGGATGCTCTCCAAACAGCTCATGCTACTGCTGCTGCTGCTTCTAATGGTGCTCACAATCCAACATTGGGAAGAAATGGCACTGTCCTTACCTTTGGTTCAGATTCTCCTCTTTGTGAATCAATGGCTTCTGTATTGAACCTTGCCGAGAAAACACAAAACTCTCTTCAGAATGGTTTTCATGTACCTGATCAACAAAGAATTCTGGTTCCTTCCATAACTGGAGATAATGGGGATGACCAATCAAGTGGATCGTCTAATGTTACAGCTTCGAATTCATCAGAGGGGGGATGCAAAGCTGGTATACAAGAACCAGCGGTTCACAACTGTCAAAGCTTCTCTCCTCATCAAGTACCATGCTTTCCAGGCTCCCCTTGGCCTTATCCATGGAACTCACCTCAGTGGACCTCTGCAATGCCTGCGCCTGCATTCTTCCATTCTGGGTTTCCCATATCATTCTACCCTGCACCACCATACTGGGGCTGCACTGGACCCGGCTCTTGGAATGTACCATGTAATTCTCCACCATCCTCCAATCTTAGCCGCTGTGGTACAAGCTCTGGTCCAAATTCCCCGACCTTAGGGAAACATCCAAGAGACGGGGACATTCTTAATCAAGCCAGCTCTCAAAAAGAAGAGTCCAGCAGAGATAACAGGTCAGATAGATGTGTTTTGATTCCGAAAACGTTAAGGATTGATGATCCTAGTGAAGCTGCAAAGAGCTCTATATGGGAAACACTTGGCATCAAGAATGAAAAGGGAAATTCTATCAACGAGGGAGGTATCTTCAAGTCATTTGAATCCAAGGGACACGAGAAAAGGCAAGTTGTTGAACAATCTTCGGTATTGCAAGCTAATCCTGCAGCCTTTTCCCGGTCACTCAACTTCCGTGAGATCTCATGA

Protein Analysis

526

Amino Acids

56.52

Weight (kDa)

5.82

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 158 - 214 1.4e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 64
AccI GTMKAC 1 cut(s) 982
AciI CCGC 4 cut(s) 104, 550, 995, 1222
AclI AACGTT 1 cut(s) 1364
AclWI GGATC 2 cut(s) 937, 1373
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 5 cut(s) 266, 879, 952, 1246, 1441
AcuI CTGAAG 4 cut(s) 249, 372, 479, 830
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 4 cut(s) 864, 1032, 1188, 1231
AfiI CCNNNNNNNGG 8 cut(s) 74, 110, 746, 1157, 1179, 1259, 1277, 1551
AflIII ACRYGT 1 cut(s) 669
AgsI TTSAA 6 cut(s) 176, 322, 817, 1468, 1478, 1511
AjnI CCWGG 1 cut(s) 1042
AleI CACNNNNGTG 1 cut(s) 1078
AloI GAACNNNNNNTCC 2 cut(s) 497, 529
Alw21I GWGCWC 2 cut(s) 733, 1403
Alw26I GTCTC 2 cut(s) 425, 1269
AlwI GGATC 2 cut(s) 937, 1373
AoxI GGCC 2 cut(s) 76, 1055
ApeKI GCWGC 9 cut(s) 146, 149, 152, 710, 713, 716, 1161, 1391, 1541
ApoI RAATTY 5 cut(s) 266, 879, 952, 1246, 1441
ArsI GACNNNNNNTTYG 2 cut(s) 37, 69
AspLEI GCGC 2 cut(s) 67, 1102
AspS9I GGNCC 3 cut(s) 1083, 1169, 1241
AsuC2I CCSGG 2 cut(s) 1173, 1552
AsuHPI GGTGA 1 cut(s) 1065
AsuII TTCGAA 1 cut(s) 950
AvaII GGWCC 3 cut(s) 1083, 1169, 1241
AxyI CCTNAGG 1 cut(s) 1258
BaeI ACNNNNGTAYC 2 cut(s) 375, 408
BanI GGYRCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 1403
BauI CACGAG 1 cut(s) 1490
BbsI GAAGAC 2 cut(s) 237, 321
Bbv12I GWGCWC 2 cut(s) 733, 1403
BbvCI CCTCAGC 1 cut(s) 370
BbvI GCAGC 9 cut(s) 133, 136, 139, 697, 700, 703, 1148, 1378, 1553
BccI CCATC 1 cut(s) 1213
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 1044
BclI TGATCA 1 cut(s) 868
BcnI CCSGG 2 cut(s) 1173, 1552
BcoDI GTCTC 2 cut(s) 425, 1269
BfaI CTAG 1 cut(s) 1383
BfmI CTRYAG 1 cut(s) 1539
BfoI RGCGCY 1 cut(s) 68
BglII AGATCT 1 cut(s) 1572
Bme1390I CCNGG 3 cut(s) 1044, 1173, 1552
Bme18I GGWCC 3 cut(s) 1083, 1169, 1241
BmgT120I GGNCC 3 cut(s) 1083, 1169, 1241
BmiI GGNNCC 5 cut(s) 66, 87, 888, 1048, 1171
BmrFI CCNGG 3 cut(s) 1044, 1173, 1552
BmrI ACTGGG 1 cut(s) 1165
BmsI GCATC 3 cut(s) 673, 959, 1431
BmuI ACTGGG 1 cut(s) 1165
BpiI GAAGAC 2 cut(s) 237, 321
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 1 cut(s) 370
Bpu14I TTCGAA 1 cut(s) 950
BpuMI CCSGG 2 cut(s) 1173, 1552
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 3 cut(s) 1052, 1064, 1482
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 8 cut(s) 74, 110, 746, 1157, 1179, 1259, 1277, 1551
Bse1I ACTGG 5 cut(s) 435, 584, 904, 1160, 1171
Bse21I CCTNAGG 1 cut(s) 1258
Bse3DI GCAATG 1 cut(s) 1098
BseBI CCWGG 1 cut(s) 1044
BseDI CCNNGG 3 cut(s) 1052, 1064, 1482
BseGI GGATG 6 cut(s) 292, 688, 919, 974, 1205, 1267
BseLI CCNNNNNNNGG 8 cut(s) 74, 110, 746, 1157, 1179, 1259, 1277, 1551
BseMI GCAATG 1 cut(s) 1098
BseMII CTCAG 3 cut(s) 204, 384, 1091
BseNI ACTGG 5 cut(s) 435, 584, 904, 1160, 1171
BseRI GAGGAG 2 cut(s) 777, 1011
BseXI GCAGC 9 cut(s) 133, 136, 139, 697, 700, 703, 1148, 1378, 1553
BsgI GTGCAG 2 cut(s) 1128, 1147
BshFI GGCC 2 cut(s) 78, 1057
BshNI GGYRCC 1 cut(s) 64
BsiHKAI GWGCWC 2 cut(s) 733, 1403
BsiSI CCGG 4 cut(s) 159, 588, 1173, 1552
BslFI GGGAC 3 cut(s) 471, 1295, 1500
BslI CCNNNNNNNGG 8 cut(s) 74, 110, 746, 1157, 1179, 1259, 1277, 1551
BsmAI GTCTC 2 cut(s) 425, 1269
BsmBI CGTCTC 1 cut(s) 1269
BsmFI GGGAC 3 cut(s) 471, 1295, 1500
BsmI GAATGC 1 cut(s) 1106
BsnI GGCC 2 cut(s) 78, 1057
Bso31I GGTCTC 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 950
Bsp1286I GDGCHC 2 cut(s) 733, 1403
Bsp143I GATC 6 cut(s) 289, 301, 868, 929, 1378, 1572
Bsp19I CCATGG 1 cut(s) 1064
BspACI CCGC 4 cut(s) 104, 550, 995, 1222
BspANI GGCC 2 cut(s) 78, 1057
BspCNI CTCAG 3 cut(s) 203, 383, 1090
BspHI TCATGA 2 cut(s) 597, 1577
BspLI GGNNCC 5 cut(s) 66, 87, 888, 1048, 1171
BspMAI CTGCAG 1 cut(s) 1543
BspPI GGATC 2 cut(s) 937, 1373
BspQI GCTCTTC 1 cut(s) 41
BspT104I TTCGAA 1 cut(s) 950
BspT107I GGYRCC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 1098
BsrI ACTGG 5 cut(s) 435, 584, 904, 1160, 1171
BssECI CCNNGG 3 cut(s) 1052, 1064, 1482
BssMI GATC 6 cut(s) 289, 301, 868, 929, 1378, 1572
BssNAI GTATAC 1 cut(s) 983
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 1 cut(s) 1490
BssT1I CCWWGG 3 cut(s) 1052, 1064, 1482
Bst1107I GTATAC 1 cut(s) 983
Bst2BI CACGAG 1 cut(s) 1490
Bst2UI CCWGG 1 cut(s) 1044
Bst4CI ACNGT 3 cut(s) 569, 763, 1007
Bst6I CTCTTC 4 cut(s) 41, 130, 849, 1308
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 1097
BstBI TTCGAA 1 cut(s) 950
BstC8I GCNNGC 4 cut(s) 1098, 1104, 1300, 1530
BstDEI CTNAG 9 cut(s) 6, 138, 190, 298, 370, 408, 1077, 1217, 1258
BstDSI CCRYGG 1 cut(s) 1064
BstF5I GGATG 6 cut(s) 292, 688, 919, 974, 1205, 1267
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 2 cut(s) 67, 1102
BstKTI GATC 6 cut(s) 292, 304, 871, 932, 1381, 1575
BstMAI GTCTC 2 cut(s) 425, 1269
BstMBI GATC 6 cut(s) 289, 301, 868, 929, 1378, 1572
BstMWI GCNNNNNNNGC 8 cut(s) 152, 558, 650, 710, 716, 1044, 1097, 1538
BstNI CCWGG 1 cut(s) 1044
BstNSI RCATGY 1 cut(s) 673
BstSCI CCNGG 3 cut(s) 1042, 1171, 1550
BstSFI CTRYAG 1 cut(s) 1539
BstV1I GCAGC 9 cut(s) 133, 136, 139, 697, 700, 703, 1148, 1378, 1553
BstV2I GAAGAC 2 cut(s) 237, 321
BstX2I RGATCY 1 cut(s) 1572
BstXI CCANNNNNNTGG 3 cut(s) 55, 926, 1155
BstYI RGATCY 1 cut(s) 1572
BstZ17I GTATAC 1 cut(s) 983
Bsu36I CCTNAGG 1 cut(s) 1258
BsuRI GGCC 2 cut(s) 78, 1057
BtgI CCRYGG 1 cut(s) 1064
BtsCI GGATG 6 cut(s) 292, 688, 919, 974, 1205, 1267
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 4 cut(s) 270, 759, 1085, 1164
Cac8I GCNNGC 4 cut(s) 1098, 1104, 1300, 1530
CciI TCATGA 2 cut(s) 597, 1577
CfoI GCGC 2 cut(s) 67, 1102
Cfr13I GGNCC 3 cut(s) 1083, 1169, 1241
Csp6I GTAC 4 cut(s) 863, 1031, 1187, 1230
CviQI GTAC 4 cut(s) 863, 1031, 1187, 1230
DdeI CTNAG 9 cut(s) 6, 138, 190, 298, 370, 408, 1077, 1217, 1258
DinI GGCGCC 1 cut(s) 66
DpnI GATC 6 cut(s) 291, 303, 870, 931, 1380, 1574
DpnII GATC 6 cut(s) 289, 301, 868, 929, 1378, 1572
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 4 cut(s) 41, 130, 849, 1308
EarI CTCTTC 4 cut(s) 41, 130, 849, 1308
Ecl136II GAGCTC 1 cut(s) 1401
Eco130I CCWWGG 3 cut(s) 1052, 1064, 1482
Eco24I GRGCYC 1 cut(s) 1403
Eco31I GGTCTC 1 cut(s) 425
Eco47I GGWCC 3 cut(s) 1083, 1169, 1241
Eco53kI GAGCTC 1 cut(s) 1401
Eco57I CTGAAG 4 cut(s) 249, 372, 479, 830
Eco81I CCTNAGG 1 cut(s) 1258
EcoICRI GAGCTC 1 cut(s) 1401
EcoRI GAATTC 3 cut(s) 266, 879, 952
EcoRII CCWGG 1 cut(s) 1042
EcoT14I CCWWGG 3 cut(s) 1052, 1064, 1482
EcoT38I GRGCYC 1 cut(s) 1403
EgeI GGCGCC 1 cut(s) 66
EheI GGCGCC 1 cut(s) 66
ErhI CCWWGG 3 cut(s) 1052, 1064, 1482
Esp3I CGTCTC 1 cut(s) 1269
FaqI GGGAC 3 cut(s) 471, 1295, 1500
FauI CCCGC 2 cut(s) 111, 557
FbaI TGATCA 1 cut(s) 868
FblI GTMKAC 1 cut(s) 982
FokI GGATG 6 cut(s) 279, 695, 926, 981, 1192, 1254
FriOI GRGCYC 1 cut(s) 1403
FspBI CTAG 1 cut(s) 1383
GlaI GCGC 2 cut(s) 66, 1101
GsuI CTGGAG 1 cut(s) 921
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 2 cut(s) 78, 1057
HapII CCGG 4 cut(s) 159, 588, 1173, 1552
HhaI GCGC 2 cut(s) 67, 1102
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 2 cut(s) 65, 1100
HinP1I GCGC 2 cut(s) 65, 1100
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 2 cut(s) 197, 1012
HpaII CCGG 4 cut(s) 159, 588, 1173, 1552
HphI GGTGA 1 cut(s) 1065
Hpy166II GTNNAC 4 cut(s) 133, 983, 1000, 1083
Hpy188III TCNNGA 4 cut(s) 342, 598, 1426, 1578
Hpy8I GTNNAC 4 cut(s) 133, 983, 1000, 1083
Hpy99I CGWCG 2 cut(s) 343, 630
HpyAV CCTTC 1 cut(s) 900
HpyCH4III ACNGT 3 cut(s) 569, 763, 1007
HpyCH4IV ACGT 2 cut(s) 537, 1364
HpyCH4V TGCA 9 cut(s) 561, 972, 1091, 1106, 1145, 1164, 1394, 1528, 1541
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 558, 650, 710, 716, 1044, 1097, 1538
HpyF3I CTNAG 9 cut(s) 6, 138, 190, 298, 370, 408, 1077, 1217, 1258
HpySE526I ACGT 2 cut(s) 537, 1364
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 2 cut(s) 65, 1100
KasI GGCGCC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 868
Kzo9I GATC 6 cut(s) 289, 301, 868, 929, 1378, 1572
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 3 cut(s) 113, 617, 1052
Lsp1109I GCAGC 9 cut(s) 133, 136, 139, 697, 700, 703, 1148, 1378, 1553
LweI GCATC 3 cut(s) 673, 959, 1431
MaeI CTAG 1 cut(s) 1383
MaeII ACGT 2 cut(s) 537, 1364
MaeIII GTNAC 4 cut(s) 518, 665, 940, 1554
MalI GATC 6 cut(s) 291, 303, 870, 931, 1380, 1574
MboI GATC 6 cut(s) 289, 301, 868, 929, 1378, 1572
MflI RGATCY 1 cut(s) 1572
MhlI GDGCHC 2 cut(s) 733, 1403
MluCI AATT 8 cut(s) 266, 305, 345, 879, 952, 1195, 1246, 1441
Mly113I GGCGCC 1 cut(s) 65
MlyI GAGTC 4 cut(s) 116, 128, 448, 1325
MmeI TCCRAC 6 cut(s) 113, 144, 353, 608, 660, 764
MseI TTAA 3 cut(s) 674, 1290, 1367
MslI CAYNNNNRTG 2 cut(s) 674, 1078
MspA1I CMGCKG 3 cut(s) 374, 995, 1224
MspI CCGG 4 cut(s) 159, 588, 1173, 1552
MspR9I CCNGG 3 cut(s) 1044, 1173, 1552
Mva1269I GAATGC 1 cut(s) 1106
MvaI CCWGG 1 cut(s) 1044
MwoI GCNNNNNNNGC 8 cut(s) 152, 558, 650, 710, 716, 1044, 1097, 1538
NarI GGCGCC 1 cut(s) 65
NciI CCSGG 2 cut(s) 1173, 1552
NcoI CCATGG 1 cut(s) 1064
NdeII GATC 6 cut(s) 289, 301, 868, 929, 1378, 1572
NlaIV GGNNCC 5 cut(s) 66, 87, 888, 1048, 1171
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 2 cut(s) 665, 1554
NspI RCATGY 1 cut(s) 673
NspV TTCGAA 1 cut(s) 950
OliI CACNNNNGTG 1 cut(s) 1078
PagI TCATGA 2 cut(s) 597, 1577
PciI ACATGT 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 41
PctI GAATGC 1 cut(s) 1106
PfeI GAWTC 6 cut(s) 355, 594, 782, 797, 1354, 1478
PleI GAGTC 4 cut(s) 116, 128, 448, 1324
PluTI GGCGCC 1 cut(s) 68
PpsI GAGTC 4 cut(s) 116, 128, 448, 1324
PscI ACATGT 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 1403
Psp1406I AACGTT 1 cut(s) 1364
Psp6I CCWGG 1 cut(s) 1042
PspGI CCWGG 1 cut(s) 1042
PspN4I GGNNCC 5 cut(s) 66, 87, 888, 1048, 1171
PspPI GGNCC 3 cut(s) 1083, 1169, 1241
PstI CTGCAG 1 cut(s) 1543
PsuI RGATCY 1 cut(s) 1572
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 4 cut(s) 864, 1032, 1188, 1231
RsaNI GTAC 4 cut(s) 863, 1031, 1187, 1230
RseI CAYNNNNRTG 2 cut(s) 674, 1078
SacI GAGCTC 1 cut(s) 1403
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 3 cut(s) 674, 1290, 1367
Sau3AI GATC 6 cut(s) 289, 301, 868, 929, 1378, 1572
Sau96I GGNCC 3 cut(s) 1083, 1169, 1241
SchI GAGTC 4 cut(s) 116, 128, 448, 1325
ScrFI CCNGG 3 cut(s) 1044, 1173, 1552
SduI GDGCHC 2 cut(s) 733, 1403
SfaNI GCATC 3 cut(s) 673, 959, 1431
SfcI CTRYAG 1 cut(s) 1539
SfoI GGCGCC 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 950
SinI GGWCC 3 cut(s) 1083, 1169, 1241
SmiMI CAYNNNNRTG 2 cut(s) 674, 1078
Sse9I AATT 8 cut(s) 266, 305, 345, 879, 952, 1195, 1246, 1441
SsiI CCGC 4 cut(s) 104, 550, 995, 1222
SspDI GGCGCC 1 cut(s) 64
SspMI CTAG 1 cut(s) 1383
SstI GAGCTC 1 cut(s) 1403
StyD4I CCNGG 3 cut(s) 1042, 1171, 1550
StyI CCWWGG 3 cut(s) 1052, 1064, 1482
TaaI ACNGT 3 cut(s) 569, 763, 1007
TaiI ACGT 2 cut(s) 540, 1367
TaqI TCGA 1 cut(s) 950
TasI AATT 8 cut(s) 266, 305, 345, 879, 952, 1195, 1246, 1441
TauI GCSGC 1 cut(s) 1224
TfiI GAWTC 6 cut(s) 355, 594, 782, 797, 1354, 1478
Tru1I TTAA 3 cut(s) 674, 1290, 1367
Tru9I TTAA 3 cut(s) 674, 1290, 1367
TscAI CASTG 4 cut(s) 277, 766, 1085, 1171
TseFI GTSAC 2 cut(s) 665, 1554
TseI GCWGC 9 cut(s) 146, 149, 152, 710, 713, 716, 1161, 1391, 1541
Tsp45I GTSAC 2 cut(s) 665, 1554
TspDTI ATGAA 5 cut(s) 27, 636, 848, 945, 1446
TspGWI ACGGA 1 cut(s) 1556
TspRI CASTG 4 cut(s) 277, 766, 1085, 1171
VpaK11BI GGWCC 3 cut(s) 1083, 1169, 1241
XapI RAATTY 5 cut(s) 266, 879, 952, 1246, 1441
XceI RCATGY 1 cut(s) 673
XcmI CCANNNNNNNNNTGG 1 cut(s) 1050
XmiI GTMKAC 1 cut(s) 982
XspI CTAG 1 cut(s) 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.