FvH4_4g32160

Ribose-5-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005658
Physical Location & Seq
Reverse (-)
30936798 .. 30937856
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32160.t1

Sequence Viewer

Length: 795 bp
ATGGAAGGTGCAATCTTGGTGTCTGCTCCGTCCTCTCCTCCCTCCTCAGCTTCTATCCCCTCCGTGATTCTGTCCCAAGATGAATTGAAGAAAATCGCCGCCTACAAGGCCGTGGAATACGTCGAGTCCGGCATGGTTCTCGGCCTCGGCACCGGCTCCACGGCCAAGCACGCCGTCGACCGAATCGGCGACCTCCTCCGCCAGGGGAAGCTCCACAACATTGTGGGCATACCCACCTCCAAGAAGACCCACGAGCAAGCTGTCTCCGTCGGAATCCCTCTCGCCGATCTCGACTCCCATCCTGTCATTGATCTCGCCATCGACGGCGCCGACGAGGTGGACCCCCACCTGAACCTGGTCAAGGGCCGAGGCGGGTCGCTGCTGAGAGAGAAGATGGTGGAGGGAGCGTGCCGGAAATTCGTGGTGATTGTTGACGAGTCGAAGCTTGTGAAGCATTTGGGGGGTAGCGGGCTGGCTATGCCGGTGGAGGTTGTGCCGTTTTGCTGGAAGTTTACGGCCAGGAGGCTGCAGAGCTTGTTTGAGGAGGCGGGTTGTGTTGCGAAGCTGAGGACTTGTTCGGGGGCGAAGGGTGAGGCTTATGTGACAGACAATGGGAACTACATTGTGGATTTGTATTTCGAGGAAGCCATTGGGGATTTGAAGGCGGCGAGCGATGCGATTTTGCAGCTTGCTGGTGTTGTGGAGCATGGGATGTTTCTGGATATGGCGACTACTGTGATTGTTGCAGGGGAGCTTGGGATAACGGTGAAGAATAACAGTAGACGAATGTTTTGA

Protein Analysis

265

Amino Acids

28.03

Weight (kDa)

5.82

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rib_5-P_isom_A PF06026 75 - 251 6.2e-63 Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 149, 326
AccI GTMKAC 2 cut(s) 177, 781
AciI CCGC 6 cut(s) 99, 199, 372, 468, 548, 665
AcoI YGGCCR 2 cut(s) 162, 516
AcsI RAATTY 1 cut(s) 416
AcyI GRCGYC 1 cut(s) 327
AfiI CCNNNNNNNGG 3 cut(s) 202, 355, 361
AgsI TTSAA 2 cut(s) 88, 661
AjnI CCWGG 3 cut(s) 201, 354, 518
AluBI AGCT 8 cut(s) 50, 211, 260, 445, 534, 565, 688, 754
AluI AGCT 8 cut(s) 50, 211, 260, 445, 534, 565, 688, 754
Alw26I GTCTC 1 cut(s) 268
AoxI GGCC 5 cut(s) 108, 142, 162, 364, 516
ApeKI GCWGC 3 cut(s) 379, 526, 685
ApoI RAATTY 1 cut(s) 416
ArsI GACNNNNNNTTYG 1 cut(s) 774
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 2 cut(s) 340, 364
AsuHPI GGTGA 3 cut(s) 436, 602, 778
AvaII GGWCC 1 cut(s) 340
BanI GGYRCC 2 cut(s) 149, 326
BauI CACGAG 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 251
BbvCI CCTCAGC 2 cut(s) 46, 566
BbvI GCAGC 3 cut(s) 366, 513, 697
BccI CCATC 3 cut(s) 306, 326, 388
BceAI ACGGC 6 cut(s) 95, 158, 177, 340, 481, 531
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciT130I CCWGG 3 cut(s) 203, 356, 520
BcoDI GTCTC 1 cut(s) 268
BfmI CTRYAG 1 cut(s) 527
BfoI RGCGCY 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 5 cut(s) 99, 380, 527, 666, 686
BlsI GCNGC 5 cut(s) 100, 381, 528, 667, 687
Bme1390I CCNGG 3 cut(s) 203, 356, 520
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 340, 364
BmiI GGNNCC 4 cut(s) 151, 157, 328, 342
BmrFI CCNGG 3 cut(s) 203, 356, 520
BmsI GCATC 1 cut(s) 664
BpiI GAAGAC 1 cut(s) 251
Bpu10I CCTNAGC 2 cut(s) 46, 566
BsaHI GRCGYC 1 cut(s) 327
BsaJI CCNNGG 5 cut(s) 111, 145, 159, 202, 367
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 355, 361
Bse118I RCCGGY 2 cut(s) 152, 481
BseBI CCWGG 3 cut(s) 203, 356, 520
BseDI CCNNGG 5 cut(s) 111, 145, 159, 202, 367
BseGI GGATG 2 cut(s) 298, 717
BseLI CCNNNNNNNGG 3 cut(s) 202, 355, 361
BseMII CTCAG 3 cut(s) 60, 374, 557
BseRI GAGGAG 4 cut(s) 27, 34, 185, 557
BseXI GCAGC 3 cut(s) 366, 513, 697
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 5 cut(s) 110, 144, 164, 366, 518
BshNI GGYRCC 2 cut(s) 149, 326
BsiEI CGRYCG 1 cut(s) 181
BsiSI CCGG 4 cut(s) 129, 153, 412, 482
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 3 cut(s) 202, 355, 361
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 5 cut(s) 110, 144, 164, 366, 518
Bsp143I GATC 2 cut(s) 286, 310
BspACI CCGC 6 cut(s) 99, 199, 372, 468, 548, 665
BspANI GGCC 5 cut(s) 110, 144, 164, 366, 518
BspCNI CTCAG 3 cut(s) 59, 375, 558
BspLI GGNNCC 4 cut(s) 151, 157, 328, 342
BspMAI CTGCAG 1 cut(s) 531
BspT107I GGYRCC 2 cut(s) 149, 326
BsrFI RCCGGY 2 cut(s) 152, 481
BssAI RCCGGY 2 cut(s) 152, 481
BssECI CCNNGG 5 cut(s) 111, 145, 159, 202, 367
BssMI GATC 2 cut(s) 286, 310
BssNI GRCGYC 1 cut(s) 327
BssSI CACGAG 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 3 cut(s) 203, 356, 520
Bst4CI ACNGT 3 cut(s) 736, 766, 779
BstACI GRCGYC 1 cut(s) 327
BstC8I GCNNGC 7 cut(s) 171, 258, 409, 470, 474, 670, 690
BstDEI CTNAG 3 cut(s) 46, 383, 566
BstDSI CCRYGG 2 cut(s) 111, 159
BstENI CCTNNNNNAGG 2 cut(s) 200, 359
BstF5I GGATG 2 cut(s) 298, 717
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 2 cut(s) 289, 313
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 2 cut(s) 286, 310
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 5 cut(s) 107, 170, 451, 478, 674
BstNI CCWGG 3 cut(s) 203, 356, 520
BstSCI CCNGG 3 cut(s) 201, 354, 518
BstSFI CTRYAG 1 cut(s) 527
BstV1I GCAGC 3 cut(s) 366, 513, 697
BstV2I GAAGAC 1 cut(s) 251
BsuRI GGCC 5 cut(s) 110, 144, 164, 366, 518
BtgI CCRYGG 2 cut(s) 111, 159
BtgZI GCGATG 1 cut(s) 687
BtsCI GGATG 2 cut(s) 298, 717
Cac8I GCNNGC 7 cut(s) 171, 258, 409, 470, 474, 670, 690
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 2 cut(s) 152, 481
Cfr13I GGNCC 2 cut(s) 340, 364
CsiI ACCWGGT 1 cut(s) 354
CviAII CATG 2 cut(s) 133, 707
DdeI CTNAG 3 cut(s) 46, 383, 566
DinI GGCGCC 1 cut(s) 328
DpnI GATC 2 cut(s) 288, 312
DpnII GATC 2 cut(s) 286, 310
EaeI YGGCCR 2 cut(s) 162, 516
EciI GGCGGA 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 340
EcoNI CCTNNNNNAGG 2 cut(s) 200, 359
EcoRII CCWGG 3 cut(s) 201, 354, 518
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
FaeI CATG 2 cut(s) 136, 710
FaiI YATR 6 cut(s) 134, 230, 479, 600, 708, 725
FaqI GGGAC 1 cut(s) 58
FatI CATG 2 cut(s) 132, 706
FauI CCCGC 3 cut(s) 365, 461, 541
FblI GTMKAC 2 cut(s) 177, 781
Fnu4HI GCNGC 5 cut(s) 99, 380, 527, 666, 686
FokI GGATG 2 cut(s) 285, 724
Fsp4HI GCNGC 5 cut(s) 99, 380, 527, 666, 686
GlaI GCGC 1 cut(s) 328
GluI GCNGC 5 cut(s) 99, 380, 527, 666, 686
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 5 cut(s) 110, 144, 164, 366, 518
HapII CCGG 4 cut(s) 129, 153, 412, 482
HhaI GCGC 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 2 cut(s) 136, 710
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 2 cut(s) 178, 433
HindII GTYRAC 2 cut(s) 178, 433
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 6 cut(s) 67, 125, 183, 273, 293, 437
HpaII CCGG 4 cut(s) 129, 153, 412, 482
HphI GGTGA 3 cut(s) 436, 602, 778
Hpy166II GTNNAC 5 cut(s) 178, 340, 433, 513, 782
Hpy188I TCNGA 1 cut(s) 272
Hpy188III TCNNGA 2 cut(s) 290, 719
Hpy8I GTNNAC 5 cut(s) 178, 340, 433, 513, 782
Hpy99I CGWCG 5 cut(s) 125, 179, 272, 326, 335
HpyAV CCTTC 2 cut(s) 580, 655
HpyCH4III ACNGT 3 cut(s) 736, 766, 779
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 4 cut(s) 11, 529, 685, 746
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 170, 451, 478, 674
HpyF3I CTNAG 3 cut(s) 46, 383, 566
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 2 cut(s) 136, 710
HspAI GCGC 1 cut(s) 327
KasI GGCGCC 1 cut(s) 326
Kzo9I GATC 2 cut(s) 286, 310
LmnI GCTCC 6 cut(s) 31, 161, 216, 404, 703, 751
Lsp1109I GCAGC 3 cut(s) 366, 513, 697
LweI GCATC 1 cut(s) 664
MabI ACCWGGT 1 cut(s) 354
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 601
MalI GATC 2 cut(s) 288, 312
MboI GATC 2 cut(s) 286, 310
MboII GAAGA 4 cut(s) 100, 256, 403, 781
MluCI AATT 2 cut(s) 83, 416
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 3 cut(s) 134, 287, 446
MmeI TCCRAC 1 cut(s) 250
MspI CCGG 4 cut(s) 129, 153, 412, 482
MspR9I CCNGG 3 cut(s) 203, 356, 520
MvaI CCWGG 3 cut(s) 203, 356, 520
MwoI GCNNNNNNNGC 5 cut(s) 107, 170, 451, 478, 674
NarI GGCGCC 1 cut(s) 327
NdeII GATC 2 cut(s) 286, 310
NlaIII CATG 2 cut(s) 136, 710
NlaIV GGNNCC 4 cut(s) 151, 157, 328, 342
NmeAIII GCCGAG 3 cut(s) 120, 126, 392
NmuCI GTSAC 1 cut(s) 601
PcsI WCGNNNNNNNCGW 3 cut(s) 288, 327, 330
PfeI GAWTC 3 cut(s) 67, 183, 273
PkrI GCNGC 5 cut(s) 100, 381, 528, 667, 687
PleI GAGTC 3 cut(s) 133, 287, 445
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 3 cut(s) 133, 287, 445
Psp6I CCWGG 3 cut(s) 201, 354, 518
PspGI CCWGG 3 cut(s) 201, 354, 518
PspN4I GGNNCC 4 cut(s) 151, 157, 328, 342
PspPI GGNCC 2 cut(s) 340, 364
PstI CTGCAG 1 cut(s) 531
SalI GTCGAC 1 cut(s) 176
SatI GCNGC 5 cut(s) 99, 380, 527, 666, 686
Sau3AI GATC 2 cut(s) 286, 310
Sau96I GGNCC 2 cut(s) 340, 364
SchI GAGTC 3 cut(s) 134, 287, 446
ScrFI CCNGG 3 cut(s) 203, 356, 520
SexAI ACCWGGT 1 cut(s) 354
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 1 cut(s) 527
SfoI GGCGCC 1 cut(s) 328
SinI GGWCC 1 cut(s) 340
Sse9I AATT 2 cut(s) 83, 416
SsiI CCGC 6 cut(s) 99, 199, 372, 468, 548, 665
SspDI GGCGCC 1 cut(s) 326
StyD4I CCNGG 3 cut(s) 201, 354, 518
TaaI ACNGT 3 cut(s) 736, 766, 779
TaiI ACGT 1 cut(s) 123
TaqI TCGA 6 cut(s) 123, 177, 291, 321, 440, 639
TaqII GACCGA 1 cut(s) 195
TasI AATT 2 cut(s) 83, 416
TauI GCSGC 2 cut(s) 101, 668
TfiI GAWTC 3 cut(s) 67, 183, 273
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 3 cut(s) 379, 526, 685
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 3 cut(s) 18, 52, 256
VpaK11BI GGWCC 1 cut(s) 340
XagI CCTNNNNNAGG 2 cut(s) 200, 359
XapI RAATTY 1 cut(s) 416
XmiI GTMKAC 2 cut(s) 177, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.