pycom13g02640

Ribose-5-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005658
Physical Location & Seq
Forward (+)
1828320 .. 1829096
777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02640.1

Sequence Viewer

Length: 777 bp
ATGGAAGCTGGGATTTTGTTGGCTCTGCCGACCTCCTCCCCCCTCTCTCCTCCTCCTCCGTCTGTGATTGTAACCCAGGACGAGTTGAAGAAAATCGCCGCCTACAAGGCCGTCGAGTATGTTGAGTCCGGAATGGTCCTCGGCCTCGGCACCGGCTCCACCGCCAAGCACGCCGTCGACCGAATAGGCGAGCTTTTGAAACAGGGGAAGCTTCAGAACATTGTAGGAATACCCACGTCGAAAAAGACCCACGAACAAGCCTTGTCTCTTGGAATTCCGCTGTCGGACCTCGATTCCCACCCTGTTCTTGATCTCGCGATCGACGGCGCCGACGAGGTCGATCCCCAACTCAATCTGGTCAAGGGCCGAGGTGGGTCCCTCTTGAGGGAGAAGATGGTGGAGGGTGCCTGTAAGAAATTCGTCTGCATCGTCGACGAGTCGAAGCTCGTGAAGCATGTGGGAGGCAGCGGGCTGGCGATGCCGGTGGAGGTTGTGCCGTTCTGCTGGAAATTCACGGCCAAAAAGCTCCAGGATTTGTTCGAGTACGCGGGCTGCGTCGGAAAGCTAAGGACTTTCGTCGAAAATGGCAAGCCTTTCGTGACGGACAATGGGAATTACATTGTGGATTTGTATTTCCAGAAAGATATGGGGGATCTGAAGGCGGCTAGCGATGCGATTTTGCAGCTTCCTGGAGTTGTGGAGCATGGAATGTTCCTTGACATGGCCACCACTGTGATTGTTGCAGGGGAGCTCGGAATCACGGTGAATAATAAGTAG

Protein Analysis

259

Amino Acids

27.54

Weight (kDa)

5.59

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rib_5-P_isom_A PF06026 75 - 250 3.3e-63 Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 149, 326, 404
AccI GTMKAC 2 cut(s) 177, 432
AccII CGCG 2 cut(s) 317, 548
AccIII TCCGGA 1 cut(s) 128
AciI CCGC 6 cut(s) 99, 162, 278, 468, 548, 662
AclWI GGATC 2 cut(s) 335, 660
AcoI YGGCCR 2 cut(s) 516, 723
AcsI RAATTY 3 cut(s) 273, 416, 509
AcuI CTGAAG 2 cut(s) 197, 677
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 1 cut(s) 545
AfiI CCNNNNNNNGG 3 cut(s) 384, 385, 721
AgsI TTSAA 2 cut(s) 88, 199
AjiI CACGTC 1 cut(s) 237
AjnI CCWGG 3 cut(s) 75, 528, 688
AleI CACNNNNGTG 1 cut(s) 731
AluBI AGCT 8 cut(s) 8, 193, 211, 445, 526, 565, 685, 751
AluI AGCT 8 cut(s) 8, 193, 211, 445, 526, 565, 685, 751
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 2 cut(s) 335, 660
Aor13HI TCCGGA 1 cut(s) 128
AoxI GGCC 5 cut(s) 108, 142, 364, 516, 723
ApeKI GCWGC 3 cut(s) 465, 552, 682
ApoI RAATTY 3 cut(s) 273, 416, 509
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 4 cut(s) 136, 286, 364, 375
AsuHPI GGTGA 1 cut(s) 775
AsuNHI GCTAGC 1 cut(s) 665
AvaII GGWCC 3 cut(s) 136, 286, 375
BalI TGGCCA 1 cut(s) 725
BanI GGYRCC 3 cut(s) 149, 326, 404
BanII GRGCYC 1 cut(s) 753
BauI CACGAG 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 753
BbvI GCAGC 3 cut(s) 477, 539, 694
BccI CCATC 1 cut(s) 388
BceAI ACGGC 5 cut(s) 95, 158, 340, 481, 531
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 3 cut(s) 77, 530, 690
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 1 cut(s) 666
BfoI RGCGCY 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 5 cut(s) 99, 466, 553, 663, 683
BlsI GCNGC 5 cut(s) 100, 467, 554, 664, 684
Bme1390I CCNGG 3 cut(s) 77, 530, 690
Bme18I GGWCC 3 cut(s) 136, 286, 375
BmgBI CACGTC 1 cut(s) 237
BmgT120I GGNCC 4 cut(s) 136, 286, 364, 375
BmiI GGNNCC 6 cut(s) 151, 157, 328, 376, 377, 406
BmrFI CCNGG 3 cut(s) 77, 530, 690
BmsI GCATC 3 cut(s) 435, 468, 661
BmtI GCTAGC 1 cut(s) 669
BoxI GACNNNNGTC 1 cut(s) 575
BpmI CTGGAG 2 cut(s) 512, 711
Bpu10I CCTNAGC 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 327
BsaJI CCNNGG 4 cut(s) 75, 139, 145, 367
BsaWI WCCGGW 1 cut(s) 128
Bsc4I CCNNNNNNNGG 3 cut(s) 384, 385, 721
Bse118I RCCGGY 2 cut(s) 152, 481
BseAI TCCGGA 1 cut(s) 128
BseBI CCWGG 3 cut(s) 77, 530, 690
BseDI CCNNGG 4 cut(s) 75, 139, 145, 367
BseLI CCNNNNNNNGG 3 cut(s) 384, 385, 721
BseRI GAGGAG 4 cut(s) 25, 39, 42, 45
BseXI GCAGC 3 cut(s) 477, 539, 694
BseYI CCCAGC 1 cut(s) 8
Bsh1236I CGCG 2 cut(s) 317, 548
Bsh1285I CGRYCG 2 cut(s) 181, 321
BshFI GGCC 5 cut(s) 110, 144, 366, 518, 725
BshNI GGYRCC 3 cut(s) 149, 326, 404
BsiEI CGRYCG 2 cut(s) 181, 321
BsiHKAI GWGCWC 1 cut(s) 753
BsiSI CCGG 3 cut(s) 129, 153, 482
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 3 cut(s) 384, 385, 721
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 5 cut(s) 110, 144, 366, 518, 725
Bsp1286I GDGCHC 1 cut(s) 753
Bsp13I TCCGGA 1 cut(s) 128
Bsp143I GATC 4 cut(s) 310, 318, 340, 652
Bsp68I TCGCGA 1 cut(s) 317
BspACI CCGC 6 cut(s) 99, 162, 278, 468, 548, 662
BspANI GGCC 5 cut(s) 110, 144, 366, 518, 725
BspEI TCCGGA 1 cut(s) 128
BspFNI CGCG 2 cut(s) 317, 548
BspLI GGNNCC 6 cut(s) 151, 157, 328, 376, 377, 406
BspOI GCTAGC 1 cut(s) 669
BspPI GGATC 2 cut(s) 335, 660
BspT107I GGYRCC 3 cut(s) 149, 326, 404
BsrFI RCCGGY 2 cut(s) 152, 481
BssAI RCCGGY 2 cut(s) 152, 481
BssECI CCNNGG 4 cut(s) 75, 139, 145, 367
BssMI GATC 4 cut(s) 310, 318, 340, 652
BssNI GRCGYC 1 cut(s) 327
BssSI CACGAG 1 cut(s) 446
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 3 cut(s) 77, 530, 690
Bst4CI ACNGT 2 cut(s) 733, 763
BstACI GRCGYC 1 cut(s) 327
BstC8I GCNNGC 7 cut(s) 171, 191, 470, 474, 550, 590, 667
BstDEI CTNAG 1 cut(s) 566
BstFNI CGCG 2 cut(s) 317, 548
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 4 cut(s) 313, 321, 343, 655
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 4 cut(s) 310, 318, 340, 652
BstMCI CGRYCG 2 cut(s) 181, 321
BstMWI GCNNNNNNNGC 5 cut(s) 107, 170, 451, 478, 671
BstNI CCWGG 3 cut(s) 77, 530, 690
BstNSI RCATGY 1 cut(s) 458
BstPAI GACNNNNGTC 1 cut(s) 575
BstSCI CCNGG 3 cut(s) 75, 528, 688
BstUI CGCG 2 cut(s) 317, 548
BstV1I GCAGC 3 cut(s) 477, 539, 694
BstX2I RGATCY 1 cut(s) 652
BstYI RGATCY 1 cut(s) 652
BsuRI GGCC 5 cut(s) 110, 144, 366, 518, 725
BtgZI GCGATG 2 cut(s) 491, 684
BtrI CACGTC 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 729
BtuMI TCGCGA 1 cut(s) 317
Cac8I GCNNGC 7 cut(s) 171, 191, 470, 474, 550, 590, 667
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 2 cut(s) 152, 481
Cfr13I GGNCC 4 cut(s) 136, 286, 364, 375
CseI GACGC 1 cut(s) 544
Csp6I GTAC 1 cut(s) 544
CspCI CAANNNNNGTGG 2 cut(s) 718, 753
CviAII CATG 3 cut(s) 455, 704, 721
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 1 cut(s) 566
DinI GGCGCC 1 cut(s) 328
DpnI GATC 4 cut(s) 312, 320, 342, 654
DpnII GATC 4 cut(s) 310, 318, 340, 652
EaeI YGGCCR 2 cut(s) 516, 723
Ecl136II GAGCTC 1 cut(s) 751
Eco24I GRGCYC 1 cut(s) 753
Eco47I GGWCC 3 cut(s) 136, 286, 375
Eco53kI GAGCTC 1 cut(s) 751
Eco57I CTGAAG 2 cut(s) 197, 677
EcoICRI GAGCTC 1 cut(s) 751
EcoO109I RGGNCCY 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 273
EcoRII CCWGG 3 cut(s) 75, 528, 688
EcoT38I GRGCYC 1 cut(s) 753
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
FaeI CATG 3 cut(s) 458, 707, 724
FaiI YATR 5 cut(s) 120, 456, 647, 705, 722
FaqI GGGAC 1 cut(s) 361
FatI CATG 3 cut(s) 454, 703, 720
FauI CCCGC 2 cut(s) 461, 541
FblI GTMKAC 2 cut(s) 177, 432
Fnu4HI GCNGC 5 cut(s) 99, 466, 553, 663, 683
FriOI GRGCYC 1 cut(s) 753
Fsp4HI GCNGC 5 cut(s) 99, 466, 553, 663, 683
FspBI CTAG 1 cut(s) 666
GlaI GCGC 1 cut(s) 328
GluI GCNGC 5 cut(s) 99, 466, 553, 663, 683
GsaI CCCAGC 1 cut(s) 12
GsuI CTGGAG 2 cut(s) 512, 711
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 5 cut(s) 110, 144, 366, 518, 725
HapII CCGG 3 cut(s) 129, 153, 482
HgaI GACGC 1 cut(s) 544
HhaI GCGC 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 3 cut(s) 458, 707, 724
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 2 cut(s) 178, 433
HindII GTYRAC 2 cut(s) 178, 433
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 4 cut(s) 125, 293, 437, 756
HpaII CCGG 3 cut(s) 129, 153, 482
HphI GGTGA 1 cut(s) 775
Hpy166II GTNNAC 2 cut(s) 178, 433
Hpy188I TCNGA 5 cut(s) 216, 286, 560, 657, 755
Hpy188III TCNNGA 7 cut(s) 129, 308, 316, 382, 448, 598, 637
Hpy8I GTNNAC 2 cut(s) 178, 433
Hpy99I CGWCG 9 cut(s) 116, 179, 241, 326, 335, 434, 437, 560, 581
HpyAV CCTTC 1 cut(s) 652
HpyCH4III ACNGT 2 cut(s) 733, 763
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 3 cut(s) 426, 682, 743
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 170, 451, 478, 671
HpyF3I CTNAG 1 cut(s) 566
HpySE526I ACGT 1 cut(s) 236
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 3 cut(s) 458, 707, 724
HspAI GCGC 1 cut(s) 327
KasI GGCGCC 1 cut(s) 326
KflI GGGWCCC 1 cut(s) 375
Kpn2I TCCGGA 1 cut(s) 128
Kzo9I GATC 4 cut(s) 310, 318, 340, 652
LmnI GCTCC 4 cut(s) 161, 531, 700, 748
Lsp1109I GCAGC 3 cut(s) 477, 539, 694
LweI GCATC 3 cut(s) 435, 468, 661
MaeI CTAG 1 cut(s) 666
MaeII ACGT 1 cut(s) 236
MaeIII GTNAC 2 cut(s) 70, 598
MalI GATC 4 cut(s) 312, 320, 342, 654
MboI GATC 4 cut(s) 310, 318, 340, 652
MboII GAAGA 2 cut(s) 100, 403
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 1 cut(s) 753
MlsI TGGCCA 1 cut(s) 725
MluCI AATT 4 cut(s) 273, 416, 509, 613
MluNI TGGCCA 1 cut(s) 725
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 2 cut(s) 134, 446
MmeI TCCRAC 2 cut(s) 264, 538
Mox20I TGGCCA 1 cut(s) 725
MroI TCCGGA 1 cut(s) 128
MscI TGGCCA 1 cut(s) 725
MslI CAYNNNNRTG 1 cut(s) 731
Msp20I TGGCCA 1 cut(s) 725
MspA1I CMGCKG 2 cut(s) 280, 468
MspI CCGG 3 cut(s) 129, 153, 482
MspR9I CCNGG 3 cut(s) 77, 530, 690
MvaI CCWGG 3 cut(s) 77, 530, 690
MvnI CGCG 2 cut(s) 317, 548
MwoI GCNNNNNNNGC 5 cut(s) 107, 170, 451, 478, 671
NarI GGCGCC 1 cut(s) 327
NdeII GATC 4 cut(s) 310, 318, 340, 652
NheI GCTAGC 1 cut(s) 665
NlaIII CATG 3 cut(s) 458, 707, 724
NlaIV GGNNCC 6 cut(s) 151, 157, 328, 376, 377, 406
NmeAIII GCCGAG 3 cut(s) 120, 126, 392
NmuCI GTSAC 1 cut(s) 598
NruI TCGCGA 1 cut(s) 317
NspI RCATGY 1 cut(s) 458
OliI CACNNNNGTG 1 cut(s) 731
PcsI WCGNNNNNNNCGW 4 cut(s) 327, 330, 426, 552
PfeI GAWTC 2 cut(s) 293, 756
PflFI GACNNNGTC 1 cut(s) 335
PfoI TCCNGGA 2 cut(s) 528, 688
PkrI GCNGC 5 cut(s) 100, 467, 554, 664, 684
Ple19I CGATCG 1 cut(s) 321
PleI GAGTC 2 cut(s) 133, 445
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 2 cut(s) 133, 445
PpuMI RGGWCCY 1 cut(s) 375
PshAI GACNNNNGTC 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 753
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 3 cut(s) 75, 528, 688
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 3 cut(s) 75, 528, 688
PspN4I GGNNCC 6 cut(s) 151, 157, 328, 376, 377, 406
PspPI GGNCC 4 cut(s) 136, 286, 364, 375
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 1 cut(s) 652
PsyI GACNNNGTC 1 cut(s) 335
PvuI CGATCG 1 cut(s) 321
RruI TCGCGA 1 cut(s) 317
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
RseI CAYNNNNRTG 1 cut(s) 731
SacI GAGCTC 1 cut(s) 753
SalI GTCGAC 2 cut(s) 176, 431
SatI GCNGC 5 cut(s) 99, 466, 553, 663, 683
Sau3AI GATC 4 cut(s) 310, 318, 340, 652
Sau96I GGNCC 4 cut(s) 136, 286, 364, 375
SchI GAGTC 2 cut(s) 134, 446
ScrFI CCNGG 3 cut(s) 77, 530, 690
SduI GDGCHC 1 cut(s) 753
SfaNI GCATC 3 cut(s) 435, 468, 661
SfoI GGCGCC 1 cut(s) 328
SgrDI CGTCGACG 1 cut(s) 431
SinI GGWCC 3 cut(s) 136, 286, 375
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 4 cut(s) 273, 416, 509, 613
SsiI CCGC 6 cut(s) 99, 162, 278, 468, 548, 662
SspDI GGCGCC 1 cut(s) 326
SspMI CTAG 1 cut(s) 666
SstI GAGCTC 1 cut(s) 753
StyD4I CCNGG 3 cut(s) 75, 528, 688
TaaI ACNGT 2 cut(s) 733, 763
TaiI ACGT 1 cut(s) 239
TaqII GACCGA 1 cut(s) 195
TasI AATT 4 cut(s) 273, 416, 509, 613
TauI GCSGC 2 cut(s) 101, 665
TfiI GAWTC 2 cut(s) 293, 756
TscAI CASTG 1 cut(s) 736
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 3 cut(s) 465, 552, 682
Tsp45I GTSAC 1 cut(s) 598
TspGWI ACGGA 2 cut(s) 48, 617
TspRI CASTG 1 cut(s) 736
Tth111I GACNNNGTC 1 cut(s) 335
VpaK11BI GGWCC 3 cut(s) 136, 286, 375
XapI RAATTY 3 cut(s) 273, 416, 509
XceI RCATGY 1 cut(s) 458
XmiI GTMKAC 2 cut(s) 177, 432
XspI CTAG 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.