MD13G1028000.v1.1

Ribose-5-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005658
Physical Location & Seq
Forward (+)
2004044 .. 2004829
786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1028000.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGAAGCTGGGATTTTGTTGCCTTTGCCGACCTCCTCCTCCCCTCTCTCTCATCCTCCTCCTCCTCCGTCTGTGATTCTAACCCAGGACGAGTTGAAGAAAATCGCCGCCTACAAGGCCGTCGAGTATGTTGAGTCCGGAATGGTCCTCGGCCTCGGCACCGGCTCCACCGCCAAGCACGCCGTCGACCGAATCGGCGAGCTTTTGAAACAGGGGCAGCTTCAGAACATTGTAGGAATACCCACGTCGAAAAAGACCCACGAACAAGCCTTGTCTCTTGGAATTCCGCTGTCGGACCTCGATTCCCACCCTGTTCTTGATCTCGCGATCGACGGCGCCGACGAGGTCGACCCCCACCTCAATCTGGTCAAAGGCCGAGGTGGGTCCCTCTTGAGGGAGAAGATGGTGGAGGGTGCCTGTAAGAAATTCGTCTGCATCGTCGACGAGTCGAAGCTCGTGAAGCATGTGGGAGGCAGCGGGCTGGCGATGCCGGTGGAGGTTGTGCCGTTCTGCTGGAAATTCACTGCCAAAAAGCTCCAGGACTTGTTCGAGTACGCGGGCTGCGTCGGAAAGCTAAGGACTTTCGTCGAAAATAGCAAGCCTTTCGTGACGGACAATGGGAATTACATTGTGGATTTGTATTTCCAGAAAGATATGGGGGATCTGAAGGCGGCTAGCGATGCGATTTTGCAGCTTCCCGGAGTTGTGGAGCATGGAATGTTCCTTGACATGGCCACCACTGTGATTGTTGCAGGGGAGCTCGGAATCACGGTGAAGAATAAGTAG

Protein Analysis

262

Amino Acids

27.96

Weight (kDa)

5.81

Isoelectric Point (pI)

31.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rib_5-P_isom_A PF06026 78 - 253 5.5e-63 Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 158, 335, 413
AccI GTMKAC 3 cut(s) 186, 348, 441
AccII CGCG 2 cut(s) 326, 557
AccIII TCCGGA 1 cut(s) 137
AciI CCGC 6 cut(s) 108, 171, 287, 477, 557, 671
AclWI GGATC 1 cut(s) 669
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 3 cut(s) 282, 425, 518
AcuI CTGAAG 2 cut(s) 206, 686
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 4 cut(s) 364, 393, 394, 730
AgsI TTSAA 2 cut(s) 97, 208
AjiI CACGTC 1 cut(s) 246
AjnI CCWGG 2 cut(s) 84, 537
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 8 cut(s) 8, 202, 220, 454, 535, 574, 694, 760
AluI AGCT 8 cut(s) 8, 202, 220, 454, 535, 574, 694, 760
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 1 cut(s) 669
Aor13HI TCCGGA 1 cut(s) 137
AoxI GGCC 4 cut(s) 117, 151, 373, 732
ApeKI GCWGC 4 cut(s) 217, 474, 561, 691
ApoI RAATTY 3 cut(s) 282, 425, 518
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 3 cut(s) 145, 295, 384
AsuC2I CCSGG 1 cut(s) 699
AsuHPI GGTGA 1 cut(s) 784
AsuNHI GCTAGC 1 cut(s) 674
AvaII GGWCC 3 cut(s) 145, 295, 384
BalI TGGCCA 1 cut(s) 734
BanI GGYRCC 3 cut(s) 158, 335, 413
BanII GRGCYC 1 cut(s) 762
BauI CACGAG 1 cut(s) 455
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 4 cut(s) 229, 486, 548, 703
BccI CCATC 1 cut(s) 397
BceAI ACGGC 4 cut(s) 104, 167, 349, 490
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 2 cut(s) 86, 539
BcnI CCSGG 1 cut(s) 699
BcoDI GTCTC 1 cut(s) 279
BfaI CTAG 1 cut(s) 675
BfoI RGCGCY 1 cut(s) 339
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 6 cut(s) 108, 218, 475, 562, 672, 692
BlsI GCNGC 6 cut(s) 109, 219, 476, 563, 673, 693
Bme1390I CCNGG 3 cut(s) 86, 539, 699
Bme18I GGWCC 3 cut(s) 145, 295, 384
BmgBI CACGTC 1 cut(s) 246
BmgT120I GGNCC 3 cut(s) 145, 295, 384
BmiI GGNNCC 6 cut(s) 160, 166, 337, 385, 386, 415
BmrFI CCNGG 3 cut(s) 86, 539, 699
BmsI GCATC 3 cut(s) 444, 477, 670
BmtI GCTAGC 1 cut(s) 678
BoxI GACNNNNGTC 1 cut(s) 584
BpmI CTGGAG 1 cut(s) 521
Bpu10I CCTNAGC 1 cut(s) 575
BpuEI CTTGAG 1 cut(s) 412
BpuMI CCSGG 1 cut(s) 699
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 4 cut(s) 84, 148, 154, 376
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 4 cut(s) 364, 393, 394, 730
Bse118I RCCGGY 2 cut(s) 161, 490
BseAI TCCGGA 1 cut(s) 137
BseBI CCWGG 2 cut(s) 86, 539
BseDI CCNNGG 4 cut(s) 84, 148, 154, 376
BseGI GGATG 1 cut(s) 52
BseLI CCNNNNNNNGG 4 cut(s) 364, 393, 394, 730
BseRI GAGGAG 5 cut(s) 25, 28, 48, 51, 54
BseXI GCAGC 4 cut(s) 229, 486, 548, 703
BseYI CCCAGC 1 cut(s) 8
Bsh1236I CGCG 2 cut(s) 326, 557
Bsh1285I CGRYCG 2 cut(s) 190, 330
BshFI GGCC 4 cut(s) 119, 153, 375, 734
BshNI GGYRCC 3 cut(s) 158, 335, 413
BsiEI CGRYCG 2 cut(s) 190, 330
BsiHKAI GWGCWC 1 cut(s) 762
BsiSI CCGG 4 cut(s) 138, 162, 491, 699
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 4 cut(s) 364, 393, 394, 730
BsmAI GTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 4 cut(s) 119, 153, 375, 734
Bsp1286I GDGCHC 1 cut(s) 762
Bsp13I TCCGGA 1 cut(s) 137
Bsp143I GATC 3 cut(s) 319, 327, 661
Bsp68I TCGCGA 1 cut(s) 326
BspACI CCGC 6 cut(s) 108, 171, 287, 477, 557, 671
BspANI GGCC 4 cut(s) 119, 153, 375, 734
BspEI TCCGGA 1 cut(s) 137
BspFNI CGCG 2 cut(s) 326, 557
BspLI GGNNCC 6 cut(s) 160, 166, 337, 385, 386, 415
BspOI GCTAGC 1 cut(s) 678
BspPI GGATC 1 cut(s) 669
BspT107I GGYRCC 3 cut(s) 158, 335, 413
BsrFI RCCGGY 2 cut(s) 161, 490
BssAI RCCGGY 2 cut(s) 161, 490
BssECI CCNNGG 4 cut(s) 84, 148, 154, 376
BssMI GATC 3 cut(s) 319, 327, 661
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 1 cut(s) 455
Bst2BI CACGAG 1 cut(s) 455
Bst2UI CCWGG 2 cut(s) 86, 539
Bst4CI ACNGT 2 cut(s) 742, 772
BstACI GRCGYC 1 cut(s) 336
BstC8I GCNNGC 7 cut(s) 180, 200, 479, 483, 559, 599, 676
BstDEI CTNAG 1 cut(s) 575
BstF5I GGATG 1 cut(s) 52
BstFNI CGCG 2 cut(s) 326, 557
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 1 cut(s) 338
BstKTI GATC 3 cut(s) 322, 330, 664
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 3 cut(s) 319, 327, 661
BstMCI CGRYCG 2 cut(s) 190, 330
BstMWI GCNNNNNNNGC 5 cut(s) 116, 179, 460, 487, 680
BstNI CCWGG 2 cut(s) 86, 539
BstNSI RCATGY 1 cut(s) 467
BstPAI GACNNNNGTC 1 cut(s) 584
BstSCI CCNGG 3 cut(s) 84, 537, 697
BstUI CGCG 2 cut(s) 326, 557
BstV1I GCAGC 4 cut(s) 229, 486, 548, 703
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BsuRI GGCC 4 cut(s) 119, 153, 375, 734
BtgZI GCGATG 2 cut(s) 500, 693
BtrI CACGTC 1 cut(s) 246
BtsCI GGATG 1 cut(s) 52
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 2 cut(s) 522, 738
BtuMI TCGCGA 1 cut(s) 326
Cac8I GCNNGC 7 cut(s) 180, 200, 479, 483, 559, 599, 676
CfoI GCGC 1 cut(s) 338
Cfr10I RCCGGY 2 cut(s) 161, 490
Cfr13I GGNCC 3 cut(s) 145, 295, 384
CseI GACGC 1 cut(s) 553
Csp6I GTAC 1 cut(s) 553
CspCI CAANNNNNGTGG 2 cut(s) 727, 762
CviAII CATG 3 cut(s) 464, 713, 730
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 1 cut(s) 575
DinI GGCGCC 1 cut(s) 337
DpnI GATC 3 cut(s) 321, 329, 663
DpnII GATC 3 cut(s) 319, 327, 661
EaeI YGGCCR 1 cut(s) 732
Ecl136II GAGCTC 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 762
Eco47I GGWCC 3 cut(s) 145, 295, 384
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 2 cut(s) 206, 686
EcoICRI GAGCTC 1 cut(s) 760
EcoO109I RGGNCCY 1 cut(s) 384
EcoRI GAATTC 1 cut(s) 282
EcoRII CCWGG 2 cut(s) 84, 537
EcoT38I GRGCYC 1 cut(s) 762
EgeI GGCGCC 1 cut(s) 337
EheI GGCGCC 1 cut(s) 337
FaeI CATG 3 cut(s) 467, 716, 733
FaiI YATR 5 cut(s) 129, 465, 656, 714, 731
FaqI GGGAC 1 cut(s) 370
FatI CATG 3 cut(s) 463, 712, 729
FauI CCCGC 2 cut(s) 470, 550
FblI GTMKAC 3 cut(s) 186, 348, 441
Fnu4HI GCNGC 6 cut(s) 108, 218, 475, 562, 672, 692
FokI GGATG 1 cut(s) 39
FriOI GRGCYC 1 cut(s) 762
Fsp4HI GCNGC 6 cut(s) 108, 218, 475, 562, 672, 692
FspBI CTAG 1 cut(s) 675
GlaI GCGC 1 cut(s) 337
GluI GCNGC 6 cut(s) 108, 218, 475, 562, 672, 692
GsaI CCCAGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 521
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 4 cut(s) 119, 153, 375, 734
HapII CCGG 4 cut(s) 138, 162, 491, 699
HgaI GACGC 1 cut(s) 553
HhaI GCGC 1 cut(s) 338
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 3 cut(s) 467, 716, 733
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HincII GTYRAC 3 cut(s) 187, 349, 442
HindII GTYRAC 3 cut(s) 187, 349, 442
HinfI GANTC 6 cut(s) 76, 134, 192, 302, 446, 765
HpaII CCGG 4 cut(s) 138, 162, 491, 699
HphI GGTGA 1 cut(s) 784
Hpy166II GTNNAC 3 cut(s) 187, 349, 442
Hpy188I TCNGA 5 cut(s) 225, 295, 569, 666, 764
Hpy188III TCNNGA 7 cut(s) 138, 317, 325, 391, 457, 607, 646
Hpy8I GTNNAC 3 cut(s) 187, 349, 442
Hpy99I CGWCG 9 cut(s) 125, 188, 250, 335, 344, 443, 446, 569, 590
HpyAV CCTTC 1 cut(s) 661
HpyCH4III ACNGT 2 cut(s) 742, 772
HpyCH4IV ACGT 1 cut(s) 245
HpyCH4V TGCA 3 cut(s) 435, 691, 752
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 179, 460, 487, 680
HpyF3I CTNAG 1 cut(s) 575
HpySE526I ACGT 1 cut(s) 245
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 3 cut(s) 467, 716, 733
HspAI GCGC 1 cut(s) 336
KasI GGCGCC 1 cut(s) 335
KflI GGGWCCC 1 cut(s) 384
Kpn2I TCCGGA 1 cut(s) 137
Kzo9I GATC 3 cut(s) 319, 327, 661
LmnI GCTCC 4 cut(s) 170, 540, 709, 757
Lsp1109I GCAGC 4 cut(s) 229, 486, 548, 703
LweI GCATC 3 cut(s) 444, 477, 670
MaeI CTAG 1 cut(s) 675
MaeII ACGT 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 607
MalI GATC 3 cut(s) 321, 329, 663
MboI GATC 3 cut(s) 319, 327, 661
MboII GAAGA 2 cut(s) 109, 412
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 1 cut(s) 762
MlsI TGGCCA 1 cut(s) 734
MluCI AATT 4 cut(s) 282, 425, 518, 622
MluNI TGGCCA 1 cut(s) 734
Mly113I GGCGCC 1 cut(s) 336
MlyI GAGTC 2 cut(s) 143, 455
MmeI TCCRAC 2 cut(s) 273, 547
Mox20I TGGCCA 1 cut(s) 734
MroI TCCGGA 1 cut(s) 137
MscI TGGCCA 1 cut(s) 734
MslI CAYNNNNRTG 1 cut(s) 740
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 2 cut(s) 289, 477
MspI CCGG 4 cut(s) 138, 162, 491, 699
MspR9I CCNGG 3 cut(s) 86, 539, 699
MvaI CCWGG 2 cut(s) 86, 539
MvnI CGCG 2 cut(s) 326, 557
MwoI GCNNNNNNNGC 5 cut(s) 116, 179, 460, 487, 680
NarI GGCGCC 1 cut(s) 336
NciI CCSGG 1 cut(s) 699
NdeII GATC 3 cut(s) 319, 327, 661
NheI GCTAGC 1 cut(s) 674
NlaIII CATG 3 cut(s) 467, 716, 733
NlaIV GGNNCC 6 cut(s) 160, 166, 337, 385, 386, 415
NmeAIII GCCGAG 3 cut(s) 129, 135, 401
NmuCI GTSAC 1 cut(s) 607
NruI TCGCGA 1 cut(s) 326
NspI RCATGY 1 cut(s) 467
OliI CACNNNNGTG 1 cut(s) 740
PcsI WCGNNNNNNNCGW 4 cut(s) 336, 339, 435, 561
PfeI GAWTC 4 cut(s) 76, 192, 302, 765
PflFI GACNNNGTC 1 cut(s) 344
PfoI TCCNGGA 2 cut(s) 537, 697
PkrI GCNGC 6 cut(s) 109, 219, 476, 563, 673, 693
Ple19I CGATCG 1 cut(s) 330
PleI GAGTC 2 cut(s) 142, 454
PluTI GGCGCC 1 cut(s) 339
PpsI GAGTC 2 cut(s) 142, 454
PpuMI RGGWCCY 1 cut(s) 384
PshAI GACNNNNGTC 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 84, 537
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 2 cut(s) 84, 537
PspN4I GGNNCC 6 cut(s) 160, 166, 337, 385, 386, 415
PspPI GGNCC 3 cut(s) 145, 295, 384
PspPPI RGGWCCY 1 cut(s) 384
PsuI RGATCY 1 cut(s) 661
PsyI GACNNNGTC 1 cut(s) 344
PvuI CGATCG 1 cut(s) 330
RruI TCGCGA 1 cut(s) 326
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 740
SacI GAGCTC 1 cut(s) 762
SalI GTCGAC 3 cut(s) 185, 347, 440
SatI GCNGC 6 cut(s) 108, 218, 475, 562, 672, 692
Sau3AI GATC 3 cut(s) 319, 327, 661
Sau96I GGNCC 3 cut(s) 145, 295, 384
SchI GAGTC 2 cut(s) 143, 455
ScrFI CCNGG 3 cut(s) 86, 539, 699
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 3 cut(s) 444, 477, 670
SfoI GGCGCC 1 cut(s) 337
SgrDI CGTCGACG 1 cut(s) 440
SinI GGWCC 3 cut(s) 145, 295, 384
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 4 cut(s) 282, 425, 518, 622
SsiI CCGC 6 cut(s) 108, 171, 287, 477, 557, 671
SspDI GGCGCC 1 cut(s) 335
SspMI CTAG 1 cut(s) 675
SstI GAGCTC 1 cut(s) 762
StyD4I CCNGG 3 cut(s) 84, 537, 697
TaaI ACNGT 2 cut(s) 742, 772
TaiI ACGT 1 cut(s) 248
TaqII GACCGA 1 cut(s) 204
TasI AATT 4 cut(s) 282, 425, 518, 622
TauI GCSGC 2 cut(s) 110, 674
TfiI GAWTC 4 cut(s) 76, 192, 302, 765
TscAI CASTG 2 cut(s) 529, 745
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 4 cut(s) 217, 474, 561, 691
Tsp45I GTSAC 1 cut(s) 607
TspGWI ACGGA 2 cut(s) 57, 626
TspRI CASTG 2 cut(s) 529, 745
Tth111I GACNNNGTC 1 cut(s) 344
VpaK11BI GGWCC 3 cut(s) 145, 295, 384
XapI RAATTY 3 cut(s) 282, 425, 518
XceI RCATGY 1 cut(s) 467
XmiI GTMKAC 3 cut(s) 186, 348, 441
XspI CTAG 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.