FvH4_4g34020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008430
Physical Location & Seq
Reverse (-)
32018477 .. 32021357
2881 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34020.t1

Sequence Viewer

Length: 1038 bp
ATGGCCAAAATGACACCGTCGCCGGCAATTCTCCTCCTCACCCTCCTCCTCCTGATCACACCGCCGCCCTCCCAATCCCTCCCCTACTCCCAATACCGCACCCTCTTCTCCCTCGCCCACTCGCTCAGGACGCACGTGGCCAACCTCCGCGCCTCAAGCGGCGACCACGCCGGTTCGAACTGGGCCAGAAACATCGCCGCCAAAATGGAGAGCGGGCTGGGCCTCGTCTTCATGTGGTCGGCGGGGAGGGACTACCTCAAGAACTGCTCCTGGAGGGAGCTGCCGTACGCGGAGATGTACGGCGCCGCGTCAGAGATGAGCGAGTTGATGAGGTGGCTCGGTGAGTTGACTCGGAAGGAATCGGACTCCGAGCGATCCGCTTCGTTGCTGCATAGGCTCCTCAGAGTGTTCAGTCAGTCGGGATCTGTCAGAGAAGTGGTGAAGGCATTTCAAAAAGAGGTGGTGGAGGGCGAGCTATTGAAGGACTATCTTGAATTGGGCAGCATTGACTTTCGATACCGCACCCTCTTCTCCCTCACCCACTCGCTCATGACGCTCGTGGCCAACCTCCGCGCCTCCCGCGGCGACCATGTCGGTTCGGACCGGGCCAGAAACATTGCCGCCAAAATGGAGAGCGGGCTGGGCCTCGTCTTCATGTGTTCGGCGGGGAGGGACTACCTCAAGAACTACTCCTGGAGGGAGCTGCCGTACACGGAGATGTACGACGCCGTGTCAGAGATGAGGGAGTTGATGAGGTGGCTCGGTGAGTTGACTCGGAAGGAATCAGACTCTGAGCGATCCGCTTCGTTGCTGCATAGGCTCCTCAGAGTGTTCAGTCAGTCGGGAGTTGTCAGAGAAGTGGTGAAGGCATTTCAAAAAGAGGTGGTGGAGGGCGAGCTATTGAAGGACTGTCTTGAATTGGGCAGCAATGACTTTCGAGGTGTGCTTCAGATCCTCAAGGATTTGGGATTACAGTACAGTTCCGCCGCTAGTTCTATAAGCACCAAGAACTTTGACATCTCTACTCCAAGGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

346

Amino Acids

38.9

Weight (kDa)

8.78

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AccBSI CCGCTC 2 cut(s) 213, 636
AccII CGCG 5 cut(s) 150, 290, 308, 573, 582
AclWI GGATC 4 cut(s) 369, 430, 792, 946
AcoI YGGCCR 3 cut(s) 3, 138, 561
AcuI CTGAAG 1 cut(s) 932
AcvI CACGTG 1 cut(s) 136
AcyI GRCGYC 2 cut(s) 303, 726
AfaI GTAC 5 cut(s) 287, 299, 710, 722, 977
AgsI TTSAA 6 cut(s) 452, 481, 494, 875, 904, 917
AhdI GACNNNNNGTC 1 cut(s) 730
AjnI CCWGG 2 cut(s) 269, 692
AluBI AGCT 4 cut(s) 280, 475, 703, 898
AluI AGCT 4 cut(s) 280, 475, 703, 898
AlwI GGATC 4 cut(s) 369, 430, 792, 946
AlwNI CAGNNNCTG 1 cut(s) 791
AoxI GGCC 7 cut(s) 3, 138, 183, 220, 561, 606, 643
ApeKI GCWGC 6 cut(s) 280, 388, 501, 703, 811, 924
AspLEI GCGC 3 cut(s) 152, 305, 575
AspS9I GGNCC 5 cut(s) 183, 220, 601, 606, 643
AsuC2I CCSGG 1 cut(s) 605
AsuHPI GGTGA 6 cut(s) 31, 353, 451, 529, 776, 874
AsuII TTCGAA 1 cut(s) 176
AvaII GGWCC 1 cut(s) 601
BalI TGGCCA 3 cut(s) 5, 140, 563
BanI GGYRCC 1 cut(s) 302
BauI CACGAG 1 cut(s) 557
BbrPI CACGTG 1 cut(s) 136
BbsI GAAGAC 2 cut(s) 220, 643
BbvI GCAGC 6 cut(s) 267, 375, 513, 690, 798, 936
BceAI ACGGC 4 cut(s) 268, 316, 691, 713
BciT130I CCWGG 2 cut(s) 271, 694
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 1 cut(s) 605
BfaI CTAG 1 cut(s) 990
BfoI RGCGCY 1 cut(s) 306
Bme1390I CCNGG 3 cut(s) 271, 605, 694
Bme18I GGWCC 1 cut(s) 601
BmeRI GACNNNNNGTC 1 cut(s) 730
BmgT120I GGNCC 5 cut(s) 183, 220, 601, 606, 643
BmiI GGNNCC 3 cut(s) 304, 398, 821
BmrFI CCNGG 3 cut(s) 271, 605, 694
BmrI ACTGGG 1 cut(s) 190
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 2 cut(s) 220, 643
BpmI CTGGAG 2 cut(s) 292, 715
Bpu10I CCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 176
BpuEI CTTGAG 4 cut(s) 139, 242, 665, 941
BpuMI CCSGG 1 cut(s) 605
BsaAI YACGTR 1 cut(s) 136
BsaHI GRCGYC 2 cut(s) 303, 726
BsaJI CCNNGG 2 cut(s) 580, 1028
BsaXI ACNNNNNCTCC 4 cut(s) 269, 299, 692, 722
Bse118I RCCGGY 2 cut(s) 22, 170
Bse1I ACTGG 1 cut(s) 185
Bse3DI GCAATG 2 cut(s) 615, 934
BseBI CCWGG 2 cut(s) 271, 694
BseDI CCNNGG 2 cut(s) 580, 1028
BseMI GCAATG 2 cut(s) 615, 934
BseMII CTCAG 4 cut(s) 139, 415, 783, 838
BseNI ACTGG 1 cut(s) 185
BseRI GAGGAG 6 cut(s) 23, 26, 35, 38, 389, 812
BseXI GCAGC 6 cut(s) 267, 375, 513, 690, 798, 936
BseYI CCCAGC 2 cut(s) 217, 640
Bsh1236I CGCG 5 cut(s) 150, 290, 308, 573, 582
BshFI GGCC 7 cut(s) 5, 140, 185, 222, 563, 608, 645
BshNI GGYRCC 1 cut(s) 302
BsiSI CCGG 3 cut(s) 23, 171, 604
BsiWI CGTACG 1 cut(s) 285
BslFI GGGAC 2 cut(s) 263, 686
BsmFI GGGAC 2 cut(s) 263, 686
BsnI GGCC 7 cut(s) 5, 140, 185, 222, 563, 608, 645
Bsp119I TTCGAA 1 cut(s) 176
Bsp143I GATC 5 cut(s) 54, 374, 422, 797, 951
BspANI GGCC 7 cut(s) 5, 140, 185, 222, 563, 608, 645
BspCNI CTCAG 4 cut(s) 138, 414, 784, 837
BspFNI CGCG 5 cut(s) 150, 290, 308, 573, 582
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 3 cut(s) 304, 398, 821
BspPI GGATC 4 cut(s) 369, 430, 792, 946
BspT104I TTCGAA 1 cut(s) 176
BspT107I GGYRCC 1 cut(s) 302
BsrBI CCGCTC 2 cut(s) 213, 636
BsrDI GCAATG 2 cut(s) 615, 934
BsrFI RCCGGY 2 cut(s) 22, 170
BsrI ACTGG 1 cut(s) 185
BssAI RCCGGY 2 cut(s) 22, 170
BssECI CCNNGG 2 cut(s) 580, 1028
BssMI GATC 5 cut(s) 54, 374, 422, 797, 951
BssNI GRCGYC 2 cut(s) 303, 726
BssSI CACGAG 1 cut(s) 557
BssT1I CCWWGG 1 cut(s) 1028
Bst2BI CACGAG 1 cut(s) 557
Bst2UI CCWGG 2 cut(s) 271, 694
Bst4CI ACNGT 4 cut(s) 18, 911, 975, 980
Bst6I CTCTTC 2 cut(s) 110, 533
BstACI GRCGYC 2 cut(s) 303, 726
BstBAI YACGTR 1 cut(s) 136
BstBI TTCGAA 1 cut(s) 176
BstC8I GCNNGC 5 cut(s) 24, 215, 473, 638, 896
BstDEI CTNAG 4 cut(s) 125, 401, 792, 824
BstDSI CCRYGG 1 cut(s) 580
BstFNI CGCG 5 cut(s) 150, 290, 308, 573, 582
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 3 cut(s) 152, 305, 575
BstKTI GATC 5 cut(s) 57, 377, 425, 800, 954
BstMBI GATC 5 cut(s) 54, 374, 422, 797, 951
BstMWI GCNNNNNNNGC 8 cut(s) 130, 156, 219, 394, 553, 579, 642, 817
BstNI CCWGG 2 cut(s) 271, 694
BstSCI CCNGG 3 cut(s) 269, 603, 692
BstUI CGCG 5 cut(s) 150, 290, 308, 573, 582
BstV1I GCAGC 6 cut(s) 267, 375, 513, 690, 798, 936
BstV2I GAAGAC 2 cut(s) 220, 643
BstX2I RGATCY 2 cut(s) 422, 951
BstYI RGATCY 2 cut(s) 422, 951
BsuRI GGCC 7 cut(s) 5, 140, 185, 222, 563, 608, 645
BtgI CCRYGG 1 cut(s) 580
BtgZI GCGATG 1 cut(s) 178
Cac8I GCNNGC 5 cut(s) 24, 215, 473, 638, 896
CaiI CAGNNNCTG 1 cut(s) 791
CciI TCATGA 1 cut(s) 549
CfoI GCGC 3 cut(s) 152, 305, 575
Cfr10I RCCGGY 2 cut(s) 22, 170
Cfr13I GGNCC 5 cut(s) 183, 220, 601, 606, 643
Cfr42I CCGCGG 1 cut(s) 583
CpoI CGGWCCG 1 cut(s) 601
CseI GACGC 4 cut(s) 139, 297, 562, 734
Csp6I GTAC 5 cut(s) 286, 298, 709, 721, 976
CspI CGGWCCG 1 cut(s) 601
CviAII CATG 4 cut(s) 232, 550, 590, 655
CviQI GTAC 5 cut(s) 286, 298, 709, 721, 976
DdeI CTNAG 4 cut(s) 125, 401, 792, 824
DinI GGCGCC 1 cut(s) 304
DpnI GATC 5 cut(s) 56, 376, 424, 799, 953
DpnII GATC 5 cut(s) 54, 374, 422, 797, 951
DriI GACNNNNNGTC 1 cut(s) 730
EaeI YGGCCR 3 cut(s) 3, 138, 561
Eam1104I CTCTTC 2 cut(s) 110, 533
Eam1105I GACNNNNNGTC 1 cut(s) 730
EarI CTCTTC 2 cut(s) 110, 533
EciI GGCGGA 1 cut(s) 973
Eco130I CCWWGG 1 cut(s) 1028
Eco47I GGWCC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 932
Eco72I CACGTG 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 269, 692
EcoT14I CCWWGG 1 cut(s) 1028
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 1028
FaeI CATG 4 cut(s) 235, 553, 593, 658
FaiI YATR 7 cut(s) 233, 393, 551, 591, 656, 816, 998
FaqI GGGAC 2 cut(s) 263, 686
FatI CATG 4 cut(s) 231, 549, 589, 654
FauI CCCGC 5 cut(s) 206, 235, 587, 629, 658
FbaI TGATCA 1 cut(s) 54
FspBI CTAG 1 cut(s) 990
GlaI GCGC 3 cut(s) 151, 304, 574
GsaI CCCAGC 2 cut(s) 221, 644
GsuI CTGGAG 2 cut(s) 292, 715
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 7 cut(s) 5, 140, 185, 222, 563, 608, 645
HapII CCGG 3 cut(s) 23, 171, 604
HgaI GACGC 4 cut(s) 139, 297, 562, 734
HhaI GCGC 3 cut(s) 152, 305, 575
Hin1I GRCGYC 2 cut(s) 303, 726
Hin1II CATG 4 cut(s) 235, 553, 593, 658
Hin6I GCGC 3 cut(s) 150, 303, 573
HinP1I GCGC 3 cut(s) 150, 303, 573
HincII GTYRAC 2 cut(s) 348, 771
HindII GTYRAC 2 cut(s) 348, 771
HinfI GANTC 6 cut(s) 349, 359, 365, 772, 782, 788
HpaII CCGG 3 cut(s) 23, 171, 604
HphI GGTGA 6 cut(s) 31, 353, 451, 529, 776, 874
Hpy166II GTNNAC 3 cut(s) 348, 711, 771
Hpy188III TCNNGA 9 cut(s) 52, 127, 259, 420, 491, 550, 682, 843, 914
Hpy8I GTNNAC 3 cut(s) 348, 711, 771
Hpy99I CGWCG 2 cut(s) 22, 728
HpyAV CCTTC 6 cut(s) 349, 436, 475, 772, 859, 898
HpyCH4III ACNGT 4 cut(s) 18, 911, 975, 980
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 2 cut(s) 391, 814
HpyF10VI GCNNNNNNNGC 8 cut(s) 130, 156, 219, 394, 553, 579, 642, 817
HpyF3I CTNAG 4 cut(s) 125, 401, 792, 824
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 2 cut(s) 303, 726
Hsp92II CATG 4 cut(s) 235, 553, 593, 658
HspAI GCGC 3 cut(s) 150, 303, 573
KasI GGCGCC 1 cut(s) 302
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 54
KspI CCGCGG 1 cut(s) 583
Kzo9I GATC 5 cut(s) 54, 374, 422, 797, 951
LmnI GCTCC 5 cut(s) 272, 277, 402, 700, 825
Lsp1109I GCAGC 6 cut(s) 267, 375, 513, 690, 798, 936
MaeI CTAG 1 cut(s) 990
MaeII ACGT 1 cut(s) 135
MalI GATC 5 cut(s) 56, 376, 424, 799, 953
MbiI CCGCTC 2 cut(s) 213, 636
MboI GATC 5 cut(s) 54, 374, 422, 797, 951
MboII GAAGA 4 cut(s) 97, 220, 520, 643
MflI RGATCY 2 cut(s) 422, 951
MlsI TGGCCA 3 cut(s) 5, 140, 563
MluCI AATT 3 cut(s) 27, 494, 917
MluNI TGGCCA 3 cut(s) 5, 140, 563
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 4 cut(s) 343, 359, 766, 782
Mox20I TGGCCA 3 cut(s) 5, 140, 563
MroNI GCCGGC 1 cut(s) 22
MscI TGGCCA 3 cut(s) 5, 140, 563
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 3 cut(s) 5, 140, 563
MspA1I CMGCKG 1 cut(s) 582
MspI CCGG 3 cut(s) 23, 171, 604
MspR9I CCNGG 3 cut(s) 271, 605, 694
MvaI CCWGG 2 cut(s) 271, 694
MvnI CGCG 5 cut(s) 150, 290, 308, 573, 582
MwoI GCNNNNNNNGC 8 cut(s) 130, 156, 219, 394, 553, 579, 642, 817
NaeI GCCGGC 1 cut(s) 24
NarI GGCGCC 1 cut(s) 303
NciI CCSGG 1 cut(s) 605
NdeII GATC 5 cut(s) 54, 374, 422, 797, 951
NgoMIV GCCGGC 1 cut(s) 22
NlaIII CATG 4 cut(s) 235, 553, 593, 658
NlaIV GGNNCC 3 cut(s) 304, 398, 821
NspV TTCGAA 1 cut(s) 176
PagI TCATGA 1 cut(s) 549
PdiI GCCGGC 1 cut(s) 24
PfeI GAWTC 2 cut(s) 359, 782
Pfl23II CGTACG 1 cut(s) 285
PflFI GACNNNGTC 2 cut(s) 16, 590
PfoI TCCNGGA 2 cut(s) 269, 692
PleI GAGTC 4 cut(s) 343, 359, 766, 782
PluTI GGCGCC 1 cut(s) 306
PmaCI CACGTG 1 cut(s) 136
PmlI CACGTG 1 cut(s) 136
PpsI GAGTC 4 cut(s) 343, 359, 766, 782
Ppu21I YACGTR 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 269, 692
PspCI CACGTG 1 cut(s) 136
PspFI CCCAGC 2 cut(s) 217, 640
PspGI CCWGG 2 cut(s) 269, 692
PspLI CGTACG 1 cut(s) 285
PspN4I GGNNCC 3 cut(s) 304, 398, 821
PspPI GGNCC 5 cut(s) 183, 220, 601, 606, 643
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 2 cut(s) 422, 951
PsyI GACNNNGTC 2 cut(s) 16, 590
RsaI GTAC 5 cut(s) 287, 299, 710, 722, 977
RsaNI GTAC 5 cut(s) 286, 298, 709, 721, 976
RseI CAYNNNNRTG 1 cut(s) 716
Rsr2I CGGWCCG 1 cut(s) 601
RsrII CGGWCCG 1 cut(s) 601
SacII CCGCGG 1 cut(s) 583
Sau3AI GATC 5 cut(s) 54, 374, 422, 797, 951
Sau96I GGNCC 5 cut(s) 183, 220, 601, 606, 643
SchI GAGTC 4 cut(s) 343, 359, 766, 782
ScrFI CCNGG 3 cut(s) 271, 605, 694
SfoI GGCGCC 1 cut(s) 304
Sfr303I CCGCGG 1 cut(s) 583
SfuI TTCGAA 1 cut(s) 176
SgrBI CCGCGG 1 cut(s) 583
SinI GGWCC 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 4 cut(s) 154, 257, 680, 956
SmoI CTYRAG 4 cut(s) 154, 257, 680, 956
Sse9I AATT 3 cut(s) 27, 494, 917
SspDI GGCGCC 1 cut(s) 302
SspMI CTAG 1 cut(s) 990
StyD4I CCNGG 3 cut(s) 269, 603, 692
StyI CCWWGG 1 cut(s) 1028
TaaI ACNGT 4 cut(s) 18, 911, 975, 980
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 176, 514, 937
TasI AATT 3 cut(s) 27, 494, 917
TatI WGTACW 1 cut(s) 975
TauI GCSGC 7 cut(s) 67, 162, 200, 308, 585, 623, 989
TfiI GAWTC 2 cut(s) 359, 782
TseI GCWGC 6 cut(s) 280, 388, 501, 703, 811, 924
TspDTI ATGAA 2 cut(s) 220, 643
TspGWI ACGGA 1 cut(s) 728
Tth111I GACNNNGTC 2 cut(s) 16, 590
VpaK11BI GGWCC 1 cut(s) 601
XspI CTAG 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.