MD10G1270500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008430
Physical Location & Seq
Forward (+)
36276063 .. 36277331
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1270500.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGCGAAAACGACGCCGTCGCGCCCCACCCTTCTCTTCCTCACCGTTCTCCTCCTGACCACAGCACAGCCCTCCCAATCCTTCTCCTACTTTCAGTACCGAACCCTCTTCTCGCTCGCCCACTCGCTTATGACGCGCGTGGCTAACCTCCGCGCATCACGCGGCGACATTTCGGGATCGGAGCGGGCCAGGGCCGTCGCGTCGAAGCTGGAGCGGGGGCTCGAGATGGGCGTGTGGGGGTTCATGCGGTCCGCCGGCTGGGACTACCTCAGGAACTACGCGTGGAGGGACTTCCCCTATACGGAGGTGGTATACGCCGTTTCGGACGTGGACGAGTTGCTGAGGTGGCTGGGTGACTTGACTCAGAGGAAATCGGATTCCGATAGAGCCGCTTGGGTCGCTCAGAATTACCAAAGTATCCTCAGGGTTTCCAATTCGCTGCTCCGTCGACTCCTCAAAGTGTTCTATCATTCGGGAGCGTTGAGAGACTTGGTGAAGACGGTGCAAAGCGAGGTGGTGGAGGGCGAGCTATTGAGGGATTGTCTTGAATTGGGCTCCAATGATTTGCAAGGTGTTGTTCAGATCCTCAAGGATTTGGGATTGAAATACGGTTCCACCAACCATCAAGAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.99

Weight (kDa)

9.23

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 184, 214
AccI GTMKAC 2 cut(s) 312, 448
AccII CGCG 7 cut(s) 22, 136, 138, 153, 162, 200, 281
AciI CCGC 7 cut(s) 151, 162, 184, 214, 247, 252, 390
AclWI GGATC 2 cut(s) 184, 577
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 258, 301
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 2 cut(s) 548, 604
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 2 cut(s) 208, 529
AluI AGCT 2 cut(s) 208, 529
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 2 cut(s) 184, 577
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 2 cut(s) 186, 192
ApeKI GCWGC 1 cut(s) 439
AspLEI GCGC 3 cut(s) 24, 138, 155
AspS9I GGNCC 3 cut(s) 186, 192, 249
AsuHPI GGTGA 3 cut(s) 34, 365, 505
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 1 cut(s) 249
AxyI CCTNAGG 2 cut(s) 269, 422
BaeI ACNNNNGTAYC 2 cut(s) 400, 433
BanII GRGCYC 2 cut(s) 222, 557
BbsI GAAGAC 1 cut(s) 503
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 1 cut(s) 426
BccI CCATC 2 cut(s) 220, 630
BceAI ACGGC 2 cut(s) 179, 302
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 480
BfuI GTATCC 1 cut(s) 428
BisI GCNGC 3 cut(s) 163, 390, 440
BlsI GCNGC 3 cut(s) 164, 391, 441
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 1 cut(s) 249
BmeT110I CYCGRG 1 cut(s) 221
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 3 cut(s) 186, 192, 249
BmiI GGNNCC 2 cut(s) 556, 613
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 503
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 572
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 301
Bse118I RCCGGY 1 cut(s) 254
Bse21I CCTNAGG 2 cut(s) 269, 422
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 258, 301
BseMII CTCAG 5 cut(s) 283, 332, 377, 416, 436
BseRI GAGGAG 2 cut(s) 41, 443
BseXI GCAGC 1 cut(s) 426
BseYI CCCAGC 2 cut(s) 258, 349
Bsh1236I CGCG 7 cut(s) 22, 136, 138, 153, 162, 200, 281
BshFI GGCC 2 cut(s) 188, 194
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 2 cut(s) 275, 302
BslI CCNNNNNNNGG 2 cut(s) 258, 301
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 2 cut(s) 275, 302
BsnI GGCC 2 cut(s) 188, 194
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 222, 557
Bsp143I GATC 2 cut(s) 176, 582
BspACI CCGC 7 cut(s) 151, 162, 184, 214, 247, 252, 390
BspANI GGCC 2 cut(s) 188, 194
BspCNI CTCAG 5 cut(s) 282, 333, 376, 415, 435
BspFNI CGCG 7 cut(s) 22, 136, 138, 153, 162, 200, 281
BspLI GGNNCC 2 cut(s) 556, 613
BspPI GGATC 2 cut(s) 184, 577
BsrBI CCGCTC 2 cut(s) 184, 214
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 2 cut(s) 176, 582
BssNAI GTATAC 1 cut(s) 313
BssNI GRCGYC 1 cut(s) 14
Bst1107I GTATAC 1 cut(s) 313
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 3 cut(s) 46, 502, 611
Bst6I CTCTTC 2 cut(s) 41, 113
BstACI GRCGYC 1 cut(s) 14
BstC8I GCNNGC 4 cut(s) 117, 186, 256, 527
BstDEI CTNAG 5 cut(s) 269, 341, 363, 402, 422
BstFNI CGCG 7 cut(s) 22, 136, 138, 153, 162, 200, 281
BstHHI GCGC 3 cut(s) 24, 138, 155
BstKTI GATC 2 cut(s) 179, 585
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 2 cut(s) 176, 582
BstMWI GCNNNNNNNGC 4 cut(s) 133, 159, 346, 398
BstNI CCWGG 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 188
BstUI CGCG 7 cut(s) 22, 136, 138, 153, 162, 200, 281
BstV1I GCAGC 1 cut(s) 426
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 582
BstYI RGATCY 1 cut(s) 582
BstZ17I GTATAC 1 cut(s) 313
Bsu36I CCTNAGG 2 cut(s) 269, 422
BsuI GTATCC 1 cut(s) 428
BsuRI GGCC 2 cut(s) 188, 194
BtrI CACGTC 1 cut(s) 328
Cac8I GCNNGC 4 cut(s) 117, 186, 256, 527
CfoI GCGC 3 cut(s) 24, 138, 155
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 3 cut(s) 186, 192, 249
CpoI CGGWCCG 1 cut(s) 249
CseI GACGC 3 cut(s) 22, 142, 189
Csp6I GTAC 1 cut(s) 97
CspI CGGWCCG 1 cut(s) 249
CviAII CATG 1 cut(s) 244
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 5 cut(s) 269, 341, 363, 402, 422
DpnI GATC 2 cut(s) 178, 584
DpnII GATC 2 cut(s) 176, 582
Eam1104I CTCTTC 2 cut(s) 41, 113
EarI CTCTTC 2 cut(s) 41, 113
EciI GGCGGA 1 cut(s) 241
Eco24I GRGCYC 2 cut(s) 222, 557
Eco47I GGWCC 1 cut(s) 249
Eco81I CCTNAGG 2 cut(s) 269, 422
Eco88I CYCGRG 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 222, 557
FaeI CATG 1 cut(s) 247
FaiI YATR 5 cut(s) 131, 245, 300, 313, 634
FaqI GGGAC 2 cut(s) 275, 302
FatI CATG 1 cut(s) 243
FauI CCCGC 2 cut(s) 177, 207
FblI GTMKAC 2 cut(s) 312, 448
Fnu4HI GCNGC 3 cut(s) 163, 390, 440
FriOI GRGCYC 2 cut(s) 222, 557
Fsp4HI GCNGC 3 cut(s) 163, 390, 440
GlaI GCGC 3 cut(s) 23, 137, 154
GluI GCNGC 3 cut(s) 163, 390, 440
GsaI CCCAGC 2 cut(s) 262, 353
GsuI CTGGAG 1 cut(s) 230
HaeIII GGCC 2 cut(s) 188, 194
HapII CCGG 1 cut(s) 255
HgaI GACGC 3 cut(s) 22, 142, 189
HhaI GCGC 3 cut(s) 24, 138, 155
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 3 cut(s) 22, 136, 153
HinP1I GCGC 3 cut(s) 22, 136, 153
HincII GTYRAC 1 cut(s) 449
HindII GTYRAC 1 cut(s) 449
HinfI GANTC 3 cut(s) 361, 377, 450
HpaII CCGG 1 cut(s) 255
HphI GGTGA 3 cut(s) 34, 365, 505
Hpy166II GTNNAC 3 cut(s) 313, 331, 449
Hpy188I TCNGA 7 cut(s) 181, 325, 366, 376, 382, 405, 582
Hpy188III TCNNGA 7 cut(s) 55, 174, 223, 271, 474, 545, 626
Hpy8I GTNNAC 3 cut(s) 313, 331, 449
Hpy99I CGWCG 5 cut(s) 16, 22, 200, 205, 450
HpyAV CCTTC 2 cut(s) 41, 91
HpyCH4III ACNGT 3 cut(s) 46, 502, 611
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 2 cut(s) 505, 568
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 159, 346, 398
HpyF3I CTNAG 5 cut(s) 269, 341, 363, 402, 422
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 3 cut(s) 22, 136, 153
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 176, 582
LmnI GCTCC 5 cut(s) 181, 211, 447, 476, 560
LpnPI CCDG 9 cut(s) 68, 175, 194, 202, 244, 256, 268, 335, 409
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 1 cut(s) 164
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 353
MalI GATC 2 cut(s) 178, 584
MbiI CCGCTC 2 cut(s) 184, 214
MboI GATC 2 cut(s) 176, 582
MboII GAAGA 3 cut(s) 28, 100, 508
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 2 cut(s) 222, 557
MluCI AATT 3 cut(s) 406, 433, 548
MluI ACGCGT 1 cut(s) 279
MlyI GAGTC 2 cut(s) 355, 444
MroNI GCCGGC 1 cut(s) 254
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 7 cut(s) 22, 136, 138, 153, 162, 200, 281
MwoI GCNNNNNNNGC 4 cut(s) 133, 159, 346, 398
NaeI GCCGGC 1 cut(s) 256
NdeII GATC 2 cut(s) 176, 582
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 2 cut(s) 556, 613
NmuCI GTSAC 1 cut(s) 353
PaeR7I CTCGAG 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 377
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 3 cut(s) 164, 391, 441
PleI GAGTC 2 cut(s) 355, 444
PpsI GAGTC 2 cut(s) 355, 444
Psp6I CCWGG 1 cut(s) 188
PspFI CCCAGC 2 cut(s) 258, 349
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 556, 613
PspPI GGNCC 3 cut(s) 186, 192, 249
PsuI RGATCY 1 cut(s) 582
PsyI GACNNNGTC 1 cut(s) 16
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
Rsr2I CGGWCCG 1 cut(s) 249
RsrII CGGWCCG 1 cut(s) 249
SalI GTCGAC 1 cut(s) 447
SatI GCNGC 3 cut(s) 163, 390, 440
Sau3AI GATC 2 cut(s) 176, 582
Sau96I GGNCC 3 cut(s) 186, 192, 249
SchI GAGTC 2 cut(s) 355, 444
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 2 cut(s) 222, 557
SetI ASST 9 cut(s) 150, 210, 270, 309, 330, 347, 516, 531, 574
SfaNI GCATC 1 cut(s) 164
Sfr274I CTCGAG 1 cut(s) 221
SinI GGWCC 1 cut(s) 249
SlaI CTCGAG 1 cut(s) 221
SmlI CTYRAG 2 cut(s) 221, 587
SmoI CTYRAG 2 cut(s) 221, 587
Sse9I AATT 3 cut(s) 406, 433, 548
SsiI CCGC 7 cut(s) 151, 162, 184, 214, 247, 252, 390
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 46, 502, 611
TaiI ACGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 203, 222, 448
TasI AATT 3 cut(s) 406, 433, 548
TauI GCSGC 2 cut(s) 165, 392
TfiI GAWTC 1 cut(s) 377
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 1 cut(s) 232
TspGWI ACGGA 2 cut(s) 317, 434
Tth111I GACNNNGTC 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 249
XhoI CTCGAG 1 cut(s) 221
XmiI GTMKAC 2 cut(s) 312, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.