Rroxscaffold_1G00064150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008430
Physical Location & Seq
Forward (+)
85972443 .. 85973682
1240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064150.1

Sequence Viewer

Length: 642 bp
ATGGCCAAAACGACGCCGTCGCCGGCAATTCCCTTCCTCACCCTCCTCCTCCTGATCGCGCCGCCGCCCTCCCAATCCCTCCCCTGCTCCCAATACCGGACCCTCTTCTCCCTCGCCCACTCGCTCATGACGCGCGTGGCCAACCTCCGCGCCTCCCGCGGAGACCACGCCGGTTCGGATCGGGCCAGGAACATCGCGGCGAAAATGGAGAGCGGGCTGGGCCTCGGCGTCTGGGGGTTCATGTGGTCGGCGGGGTGGGACTACGTCAAGAACTACTCGTGGAGGGAGCTGCCGTACTCGGAGATGTACGGCGCCGCATCGGAGGCGGGCGAGTTGATGAGGTGGCTGGGTGAGTTGACTCGGAAGGAATCGGACTCCGAGCGAGCGGCTTGGATCGGTCGGAATTACCAAAATGTCTTCAGGGTCTCTACTTCGCTGCTGCGTAGGCTCCTCCGAGTGTTCAGTCAGTCGGGAACGTTGAGAGAAGTGGTGAAGGCAGTTCAAAGAGAGGTAGTGGAGGGCGAGTTATTGAAGGACTGTCTTGAATTGGGCAGCAATGACTTTGAAGGTGTGCTTCAAATCCTCAAGGATTTGGGAGCACAATACGGCTCGGCTCCTAGTTCTAAGCACCAAGATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

23.73

Weight (kDa)

8.9

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 311
AccBSI CCGCTC 2 cut(s) 213, 386
AccII CGCG 6 cut(s) 59, 133, 135, 150, 159, 197
AclI AACGTT 1 cut(s) 476
AclWI GGATC 2 cut(s) 186, 401
AcoI YGGCCR 2 cut(s) 3, 138
AcuI CTGAAG 1 cut(s) 403
AcyI GRCGYC 3 cut(s) 14, 228, 312
AfaI GTAC 2 cut(s) 296, 308
AfiI CCNNNNNNNGG 1 cut(s) 96
AgsI TTSAA 5 cut(s) 503, 532, 545, 566, 578
AjnI CCWGG 1 cut(s) 185
AluBI AGCT 1 cut(s) 289
AluI AGCT 1 cut(s) 289
Alw21I GWGCWC 1 cut(s) 601
Alw26I GTCTC 2 cut(s) 156, 430
AlwI GGATC 2 cut(s) 186, 401
AoxI GGCC 4 cut(s) 3, 138, 183, 220
ApeKI GCWGC 4 cut(s) 289, 436, 439, 552
AspLEI GCGC 4 cut(s) 61, 135, 152, 314
AspS9I GGNCC 3 cut(s) 99, 183, 220
AsuHPI GGTGA 3 cut(s) 31, 362, 502
AvaII GGWCC 1 cut(s) 99
BalI TGGCCA 2 cut(s) 5, 140
BanI GGYRCC 1 cut(s) 311
BauI CACGAG 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 601
BbvI GCAGC 4 cut(s) 276, 423, 426, 564
BceAI ACGGC 3 cut(s) 277, 325, 622
BciT130I CCWGG 1 cut(s) 187
BcoDI GTCTC 2 cut(s) 156, 430
BfaI CTAG 1 cut(s) 618
BfoI RGCGCY 1 cut(s) 315
BglII AGATCT 1 cut(s) 634
BisI GCNGC 9 cut(s) 62, 65, 198, 290, 315, 387, 437, 440, 553
BlsI GCNGC 9 cut(s) 63, 66, 199, 291, 316, 388, 438, 441, 554
Bme1390I CCNGG 1 cut(s) 187
Bme18I GGWCC 1 cut(s) 99
BmgT120I GGNCC 3 cut(s) 99, 183, 220
BmiI GGNNCC 4 cut(s) 101, 313, 449, 615
BmrFI CCNGG 1 cut(s) 187
BmsI GCATC 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 569
BsaHI GRCGYC 3 cut(s) 14, 228, 312
BsaI GGTCTC 2 cut(s) 156, 430
BsaJI CCNNGG 2 cut(s) 157, 223
BsaWI WCCGGW 1 cut(s) 96
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 509, 539
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse118I RCCGGY 2 cut(s) 22, 170
Bse3DI GCAATG 1 cut(s) 562
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 157, 223
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMI GCAATG 1 cut(s) 562
BseRI GAGGAG 3 cut(s) 35, 38, 440
BseXI GCAGC 4 cut(s) 276, 423, 426, 564
BseYI CCCAGC 2 cut(s) 217, 346
Bsh1236I CGCG 6 cut(s) 59, 133, 135, 150, 159, 197
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 4 cut(s) 5, 140, 185, 222
BshNI GGYRCC 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 601
BsiSI CCGG 3 cut(s) 23, 97, 171
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 96
BsmAI GTCTC 2 cut(s) 156, 430
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 4 cut(s) 5, 140, 185, 222
Bso31I GGTCTC 2 cut(s) 156, 430
Bsp1286I GDGCHC 1 cut(s) 601
Bsp143I GATC 4 cut(s) 54, 178, 393, 634
BspANI GGCC 4 cut(s) 5, 140, 185, 222
BspFNI CGCG 6 cut(s) 59, 133, 135, 150, 159, 197
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 4 cut(s) 101, 313, 449, 615
BspPI GGATC 2 cut(s) 186, 401
BspT107I GGYRCC 1 cut(s) 311
BspTNI GGTCTC 2 cut(s) 156, 430
BsrBI CCGCTC 2 cut(s) 213, 386
BsrDI GCAATG 1 cut(s) 562
BsrFI RCCGGY 2 cut(s) 22, 170
BssAI RCCGGY 2 cut(s) 22, 170
BssECI CCNNGG 2 cut(s) 157, 223
BssMI GATC 4 cut(s) 54, 178, 393, 634
BssNI GRCGYC 3 cut(s) 14, 228, 312
BssSI CACGAG 1 cut(s) 277
Bst2BI CACGAG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 539
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 3 cut(s) 14, 228, 312
BstC8I GCNNGC 4 cut(s) 24, 215, 328, 384
BstDEI CTNAG 1 cut(s) 624
BstDSI CCRYGG 1 cut(s) 157
BstFNI CGCG 6 cut(s) 59, 133, 135, 150, 159, 197
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 4 cut(s) 61, 135, 152, 314
BstKTI GATC 4 cut(s) 57, 181, 396, 637
BstMAI GTCTC 2 cut(s) 156, 430
BstMBI GATC 4 cut(s) 54, 178, 393, 634
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 5 cut(s) 130, 156, 219, 323, 445
BstNI CCWGG 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 185
BstUI CGCG 6 cut(s) 59, 133, 135, 150, 159, 197
BstV1I GCAGC 4 cut(s) 276, 423, 426, 564
BstV2I GAAGAC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BsuRI GGCC 4 cut(s) 5, 140, 185, 222
BtgI CCRYGG 1 cut(s) 157
BtgZI GCGATG 1 cut(s) 178
Cac8I GCNNGC 4 cut(s) 24, 215, 328, 384
CciI TCATGA 1 cut(s) 126
CfoI GCGC 4 cut(s) 61, 135, 152, 314
Cfr10I RCCGGY 2 cut(s) 22, 170
Cfr13I GGNCC 3 cut(s) 99, 183, 220
Cfr42I CCGCGG 1 cut(s) 160
CseI GACGC 3 cut(s) 22, 139, 217
Csp6I GTAC 2 cut(s) 295, 307
CviAII CATG 2 cut(s) 127, 241
CviQI GTAC 2 cut(s) 295, 307
DdeI CTNAG 1 cut(s) 624
DinI GGCGCC 1 cut(s) 313
DpnI GATC 4 cut(s) 56, 180, 395, 636
DpnII GATC 4 cut(s) 54, 178, 393, 634
EaeI YGGCCR 2 cut(s) 3, 138
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco31I GGTCTC 2 cut(s) 156, 430
Eco47I GGWCC 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 185
EgeI GGCGCC 1 cut(s) 313
EheI GGCGCC 1 cut(s) 313
FaeI CATG 2 cut(s) 130, 244
FaiI YATR 2 cut(s) 128, 242
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FaqI GGGAC 1 cut(s) 272
FatI CATG 2 cut(s) 126, 240
FauI CCCGC 4 cut(s) 164, 206, 244, 319
Fnu4HI GCNGC 9 cut(s) 62, 65, 198, 290, 315, 387, 437, 440, 553
Fsp4HI GCNGC 9 cut(s) 62, 65, 198, 290, 315, 387, 437, 440, 553
FspBI CTAG 1 cut(s) 618
GlaI GCGC 4 cut(s) 60, 134, 151, 313
GluI GCNGC 9 cut(s) 62, 65, 198, 290, 315, 387, 437, 440, 553
GsaI CCCAGC 2 cut(s) 221, 350
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 4 cut(s) 5, 140, 185, 222
HapII CCGG 3 cut(s) 23, 97, 171
HgaI GACGC 3 cut(s) 22, 139, 217
HhaI GCGC 4 cut(s) 61, 135, 152, 314
Hin1I GRCGYC 3 cut(s) 14, 228, 312
Hin1II CATG 2 cut(s) 130, 244
Hin6I GCGC 4 cut(s) 59, 133, 150, 312
HinP1I GCGC 4 cut(s) 59, 133, 150, 312
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 3 cut(s) 358, 368, 374
HpaII CCGG 3 cut(s) 23, 97, 171
HphI GGTGA 3 cut(s) 31, 362, 502
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 8 cut(s) 178, 301, 322, 363, 373, 379, 402, 455
Hpy188III TCNNGA 5 cut(s) 52, 127, 268, 471, 542
Hpy8I GTNNAC 1 cut(s) 357
Hpy99I CGWCG 2 cut(s) 16, 22
HpyAV CCTTC 5 cut(s) 43, 358, 487, 526, 560
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 2 cut(s) 264, 476
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 156, 219, 323, 445
HpyF3I CTNAG 1 cut(s) 624
HpySE526I ACGT 2 cut(s) 264, 476
Hsp92I GRCGYC 3 cut(s) 14, 228, 312
Hsp92II CATG 2 cut(s) 130, 244
HspAI GCGC 4 cut(s) 59, 133, 150, 312
KasI GGCGCC 1 cut(s) 311
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 4 cut(s) 54, 178, 393, 634
LmnI GCTCC 5 cut(s) 92, 286, 453, 596, 619
Lsp1109I GCAGC 4 cut(s) 276, 423, 426, 564
LweI GCATC 1 cut(s) 326
MaeI CTAG 1 cut(s) 618
MaeII ACGT 2 cut(s) 264, 476
MalI GATC 4 cut(s) 56, 180, 395, 636
MbiI CCGCTC 2 cut(s) 213, 386
MboI GATC 4 cut(s) 54, 178, 393, 634
MboII GAAGA 2 cut(s) 97, 409
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 1 cut(s) 601
MlsI TGGCCA 2 cut(s) 5, 140
MluCI AATT 3 cut(s) 27, 403, 545
MluNI TGGCCA 2 cut(s) 5, 140
Mly113I GGCGCC 1 cut(s) 312
MlyI GAGTC 2 cut(s) 352, 368
MmeI TCCRAC 1 cut(s) 380
Mox20I TGGCCA 2 cut(s) 5, 140
MroNI GCCGGC 1 cut(s) 22
MscI TGGCCA 2 cut(s) 5, 140
Msp20I TGGCCA 2 cut(s) 5, 140
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 3 cut(s) 23, 97, 171
MspR9I CCNGG 1 cut(s) 187
MvaI CCWGG 1 cut(s) 187
MvnI CGCG 6 cut(s) 59, 133, 135, 150, 159, 197
MwoI GCNNNNNNNGC 5 cut(s) 130, 156, 219, 323, 445
NaeI GCCGGC 1 cut(s) 24
NarI GGCGCC 1 cut(s) 312
NdeII GATC 4 cut(s) 54, 178, 393, 634
NgoMIV GCCGGC 1 cut(s) 22
NlaIII CATG 2 cut(s) 130, 244
NlaIV GGNNCC 4 cut(s) 101, 313, 449, 615
NmeAIII GCCGAG 2 cut(s) 204, 590
PagI TCATGA 1 cut(s) 126
PdiI GCCGGC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 368
PflFI GACNNNGTC 2 cut(s) 16, 263
PkrI GCNGC 9 cut(s) 63, 66, 199, 291, 316, 388, 438, 441, 554
PleI GAGTC 2 cut(s) 352, 368
PluTI GGCGCC 1 cut(s) 315
PpsI GAGTC 2 cut(s) 352, 368
Psp1406I AACGTT 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 185
PspFI CCCAGC 2 cut(s) 217, 346
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 4 cut(s) 101, 313, 449, 615
PspPI GGNCC 3 cut(s) 99, 183, 220
PsuI RGATCY 1 cut(s) 634
PsyI GACNNNGTC 2 cut(s) 16, 263
RsaI GTAC 2 cut(s) 296, 308
RsaNI GTAC 2 cut(s) 295, 307
SacII CCGCGG 1 cut(s) 160
SatI GCNGC 9 cut(s) 62, 65, 198, 290, 315, 387, 437, 440, 553
Sau3AI GATC 4 cut(s) 54, 178, 393, 634
Sau96I GGNCC 3 cut(s) 99, 183, 220
SchI GAGTC 2 cut(s) 352, 368
ScrFI CCNGG 1 cut(s) 187
SduI GDGCHC 1 cut(s) 601
SetI ASST 7 cut(s) 147, 267, 291, 344, 479, 513, 571
SfaNI GCATC 1 cut(s) 326
SfoI GGCGCC 1 cut(s) 313
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 3 cut(s) 27, 403, 545
SspDI GGCGCC 1 cut(s) 311
SspMI CTAG 1 cut(s) 618
StyD4I CCNGG 1 cut(s) 185
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 2 cut(s) 267, 479
TaqII GACCGA 1 cut(s) 386
TasI AATT 3 cut(s) 27, 403, 545
TauI GCSGC 5 cut(s) 64, 67, 200, 317, 389
TfiI GAWTC 1 cut(s) 368
TseI GCWGC 4 cut(s) 289, 436, 439, 552
TspDTI ATGAA 1 cut(s) 229
Tth111I GACNNNGTC 2 cut(s) 16, 263
VpaK11BI GGWCC 1 cut(s) 99
XspI CTAG 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.