MD00G1093500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008430
Physical Location & Seq
Forward (+)
19620874 .. 19622190
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1093500.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGCCAAGACGACACCGTCGCACCCGACGCTTCTCTTCCTCACCCTTCTCCGCCTGACCACAGCACAGCCCTCCCAATCCTTCTCCTACTCTCTGTACCCAACCCTCTTCTCCCTCGCCCACTCGCTCATGACGCGGGTGGCCAACCTCCGCGAGGCACGCGGCGACGTTTCGGGATCGCAGCGGGCCAGGGCCGTCTCGTCGAAGCTGGAGCGGGGGCTCGGGATGGGTGTGTGGGGGTTCATGTGGTCCGCCGGCTGGGACGATCTCAGGAACTACGCGCGGAGGGACTTTCCCTATTCGGAGGTAGACGGCGCCGTTGCGGACGCCAACGAGTTGCTGAGGTGGCTGGGTGACTTGACACAGAGGCAATCGGATTCCGATCGAGCCGTTTGGGTCGCGCAGAATTACCAAACTATCCTCAAGGTTTCCAATTCGCTGCTCCGTCGGCTCCTCAAAGTGCTCTACCACTCGGAAACGTTGAGAGAAATGGTGAAGGCAGTGCAGAGAGAGGTGGTGGAAGGTGAGCTATTGGGGGATTGTCTTGTATTGGGCTCCAATGATTTGAACGGTGTGATTCAAATCCTCAAGGATTTGGGATCGCAATACGGTTCCACTACCCCACCAAGAACTTTGACACTTTTAAGAGCTATATCTTTGTTTGTAGCTTGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

25.11

Weight (kDa)

9.19

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 309
AccII CGCG 6 cut(s) 136, 153, 162, 281, 283, 401
AciI CCGC 9 cut(s) 52, 136, 151, 162, 184, 214, 252, 283, 323
AclI AACGTT 1 cut(s) 479
AclWI GGATC 2 cut(s) 184, 607
AcoI YGGCCR 2 cut(s) 3, 141
AcyI GRCGYC 2 cut(s) 315, 327
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 154, 258
AgsI TTSAA 2 cut(s) 568, 581
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 4 cut(s) 208, 529, 650, 668
AluI AGCT 4 cut(s) 208, 529, 650, 668
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 1 cut(s) 202
AlwI GGATC 2 cut(s) 184, 607
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 4 cut(s) 3, 141, 186, 192
ApeKI GCWGC 2 cut(s) 181, 439
AspLEI GCGC 3 cut(s) 283, 317, 403
AspS9I GGNCC 3 cut(s) 186, 192, 249
AsuHPI GGTGA 4 cut(s) 34, 365, 505, 536
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 1 cut(s) 249
BalI TGGCCA 2 cut(s) 5, 143
BanI GGYRCC 1 cut(s) 314
BanII GRGCYC 2 cut(s) 222, 557
Bbv12I GWGCWC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 2 cut(s) 193, 426
BccI CCATC 1 cut(s) 220
BceAI ACGGC 4 cut(s) 179, 302, 328, 374
BciT130I CCWGG 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 202
BfoI RGCGCY 1 cut(s) 318
BisI GCNGC 3 cut(s) 163, 182, 440
BlsI GCNGC 3 cut(s) 164, 183, 441
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 1 cut(s) 249
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 3 cut(s) 186, 192, 249
BmiI GGNNCC 4 cut(s) 316, 452, 556, 613
BmrFI CCNGG 1 cut(s) 190
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 341
BpuEI CTTGAG 2 cut(s) 407, 572
BsaBI GATNNNNATC 2 cut(s) 381, 581
BsaHI GRCGYC 2 cut(s) 315, 327
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 258
Bse118I RCCGGY 1 cut(s) 254
Bse8I GATNNNNATC 2 cut(s) 381, 581
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 1 cut(s) 231
BseJI GATNNNNATC 2 cut(s) 381, 581
BseLI CCNNNNNNNGG 2 cut(s) 154, 258
BseMII CTCAG 2 cut(s) 283, 332
BseRI GAGGAG 1 cut(s) 443
BseXI GCAGC 2 cut(s) 193, 426
BseYI CCCAGC 2 cut(s) 258, 349
BsgI GTGCAG 1 cut(s) 524
Bsh1236I CGCG 6 cut(s) 136, 153, 162, 281, 283, 401
Bsh1285I CGRYCG 1 cut(s) 385
BshFI GGCC 4 cut(s) 5, 143, 188, 194
BshNI GGYRCC 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 465
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 2 cut(s) 275, 302
BslI CCNNNNNNNGG 2 cut(s) 154, 258
BsmAI GTCTC 1 cut(s) 202
BsmBI CGTCTC 1 cut(s) 202
BsmFI GGGAC 2 cut(s) 275, 302
BsnI GGCC 4 cut(s) 5, 143, 188, 194
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 3 cut(s) 222, 465, 557
Bsp143I GATC 4 cut(s) 176, 265, 382, 599
BspACI CCGC 9 cut(s) 52, 136, 151, 162, 184, 214, 252, 283, 323
BspANI GGCC 4 cut(s) 5, 143, 188, 194
BspCNI CTCAG 2 cut(s) 282, 333
BspFNI CGCG 6 cut(s) 136, 153, 162, 281, 283, 401
BspHI TCATGA 1 cut(s) 129
BspLI GGNNCC 4 cut(s) 316, 452, 556, 613
BspPI GGATC 2 cut(s) 184, 607
BspT107I GGYRCC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 4 cut(s) 176, 265, 382, 599
BssNI GRCGYC 2 cut(s) 315, 327
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 3 cut(s) 18, 572, 611
Bst6I CTCTTC 2 cut(s) 41, 113
BstACI GRCGYC 2 cut(s) 315, 327
BstC8I GCNNGC 3 cut(s) 160, 186, 256
BstDEI CTNAG 2 cut(s) 269, 341
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 1 cut(s) 231
BstFNI CGCG 6 cut(s) 136, 153, 162, 281, 283, 401
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 3 cut(s) 283, 317, 403
BstKTI GATC 4 cut(s) 179, 268, 385, 602
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 4 cut(s) 176, 265, 382, 599
BstMCI CGRYCG 1 cut(s) 385
BstMWI GCNNNNNNNGC 5 cut(s) 28, 133, 159, 346, 448
BstNI CCWGG 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 188
BstUI CGCG 6 cut(s) 136, 153, 162, 281, 283, 401
BstV1I GCAGC 2 cut(s) 193, 426
BsuRI GGCC 4 cut(s) 5, 143, 188, 194
BtsCI GGATG 1 cut(s) 231
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 1 cut(s) 507
Cac8I GCNNGC 3 cut(s) 160, 186, 256
CciI TCATGA 1 cut(s) 129
CfoI GCGC 3 cut(s) 283, 317, 403
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 3 cut(s) 186, 192, 249
CseI GACGC 3 cut(s) 37, 142, 335
Csp6I GTAC 1 cut(s) 97
CviAII CATG 2 cut(s) 130, 244
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 2 cut(s) 269, 341
DinI GGCGCC 1 cut(s) 316
DpnI GATC 4 cut(s) 178, 267, 384, 601
DpnII GATC 4 cut(s) 176, 265, 382, 599
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 2 cut(s) 3, 141
Eam1104I CTCTTC 2 cut(s) 41, 113
EarI CTCTTC 2 cut(s) 41, 113
EciI GGCGGA 2 cut(s) 41, 241
Eco24I GRGCYC 2 cut(s) 222, 557
Eco47I GGWCC 1 cut(s) 249
Eco88I CYCGRG 1 cut(s) 221
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 222, 557
EgeI GGCGCC 1 cut(s) 316
EheI GGCGCC 1 cut(s) 316
Esp3I CGTCTC 1 cut(s) 202
FaeI CATG 2 cut(s) 133, 247
FaiI YATR 3 cut(s) 131, 245, 653
FaqI GGGAC 2 cut(s) 275, 302
FatI CATG 2 cut(s) 129, 243
FauI CCCGC 3 cut(s) 129, 177, 207
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 163, 182, 440
FokI GGATG 1 cut(s) 238
FriOI GRGCYC 2 cut(s) 222, 557
Fsp4HI GCNGC 3 cut(s) 163, 182, 440
GlaI GCGC 3 cut(s) 282, 316, 402
GluI GCNGC 3 cut(s) 163, 182, 440
GsaI CCCAGC 2 cut(s) 262, 353
GsuI CTGGAG 1 cut(s) 230
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 4 cut(s) 5, 143, 188, 194
HapII CCGG 1 cut(s) 255
HgaI GACGC 3 cut(s) 37, 142, 335
HhaI GCGC 3 cut(s) 283, 317, 403
Hin1I GRCGYC 2 cut(s) 315, 327
Hin1II CATG 2 cut(s) 133, 247
Hin6I GCGC 3 cut(s) 281, 315, 401
HinP1I GCGC 3 cut(s) 281, 315, 401
HinfI GANTC 2 cut(s) 377, 577
HpaII CCGG 1 cut(s) 255
HphI GGTGA 4 cut(s) 34, 365, 505, 536
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 4 cut(s) 304, 376, 382, 475
Hpy188III TCNNGA 4 cut(s) 130, 174, 223, 271
Hpy8I GTNNAC 1 cut(s) 310
Hpy99I CGWCG 5 cut(s) 22, 31, 170, 205, 450
HpyAV CCTTC 4 cut(s) 56, 91, 490, 515
HpyCH4III ACNGT 3 cut(s) 18, 572, 611
HpyCH4IV ACGT 2 cut(s) 168, 479
HpyCH4V TGCA 1 cut(s) 505
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 133, 159, 346, 448
HpyF3I CTNAG 2 cut(s) 269, 341
HpySE526I ACGT 2 cut(s) 168, 479
Hsp92I GRCGYC 2 cut(s) 315, 327
Hsp92II CATG 2 cut(s) 133, 247
HspAI GCGC 3 cut(s) 281, 315, 401
KasI GGCGCC 1 cut(s) 314
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 4 cut(s) 176, 265, 382, 599
LmnI GCTCC 4 cut(s) 211, 447, 456, 560
LpnPI CCDG 8 cut(s) 68, 175, 194, 202, 244, 256, 268, 335
Lsp1109I GCAGC 2 cut(s) 193, 426
MaeII ACGT 2 cut(s) 168, 479
MaeIII GTNAC 1 cut(s) 353
MalI GATC 4 cut(s) 178, 267, 384, 601
MbiI CCGCTC 1 cut(s) 214
MboI GATC 4 cut(s) 176, 265, 382, 599
MboII GAAGA 2 cut(s) 28, 100
MhlI GDGCHC 3 cut(s) 222, 465, 557
MlsI TGGCCA 2 cut(s) 5, 143
MluCI AATT 3 cut(s) 406, 433, 673
MluNI TGGCCA 2 cut(s) 5, 143
Mly113I GGCGCC 1 cut(s) 315
Mox20I TGGCCA 2 cut(s) 5, 143
MroNI GCCGGC 1 cut(s) 254
MscI TGGCCA 2 cut(s) 5, 143
MseI TTAA 1 cut(s) 644
Msp20I TGGCCA 2 cut(s) 5, 143
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 6 cut(s) 136, 153, 162, 281, 283, 401
MwoI GCNNNNNNNGC 5 cut(s) 28, 133, 159, 346, 448
NaeI GCCGGC 1 cut(s) 256
NarI GGCGCC 1 cut(s) 315
NdeII GATC 4 cut(s) 176, 265, 382, 599
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 2 cut(s) 133, 247
NlaIV GGNNCC 4 cut(s) 316, 452, 556, 613
NmuCI GTSAC 1 cut(s) 353
PagI TCATGA 1 cut(s) 129
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 377, 577
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 3 cut(s) 164, 183, 441
Ple19I CGATCG 1 cut(s) 385
PluTI GGCGCC 1 cut(s) 318
Psp1406I AACGTT 1 cut(s) 479
Psp6I CCWGG 1 cut(s) 188
PspFI CCCAGC 2 cut(s) 258, 349
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 4 cut(s) 316, 452, 556, 613
PspPI GGNCC 3 cut(s) 186, 192, 249
PsyI GACNNNGTC 1 cut(s) 16
PvuI CGATCG 1 cut(s) 385
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SaqAI TTAA 1 cut(s) 644
SatI GCNGC 3 cut(s) 163, 182, 440
Sau3AI GATC 4 cut(s) 176, 265, 382, 599
Sau96I GGNCC 3 cut(s) 186, 192, 249
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 3 cut(s) 222, 465, 557
SfoI GGCGCC 1 cut(s) 316
SinI GGWCC 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 422, 587
SmoI CTYRAG 2 cut(s) 422, 587
Sse9I AATT 3 cut(s) 406, 433, 673
SsiI CCGC 9 cut(s) 52, 136, 151, 162, 184, 214, 252, 283, 323
SspDI GGCGCC 1 cut(s) 314
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 18, 572, 611
TaiI ACGT 2 cut(s) 171, 482
TaqI TCGA 2 cut(s) 203, 385
TasI AATT 3 cut(s) 406, 433, 673
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 2 cut(s) 377, 577
Tru1I TTAA 1 cut(s) 644
Tru9I TTAA 1 cut(s) 644
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 2 cut(s) 181, 439
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 1 cut(s) 232
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 1 cut(s) 507
Tth111I GACNNNGTC 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 249
XagI CCTNNNNNAGG 1 cut(s) 152
XmiI GTMKAC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.