Rmu_sc0023128.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023128.1
OG0008430
Physical Location & Seq
Reverse (-)
1 .. 980
980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023128.1_g000001.1.cds

Sequence Viewer

Length: 471 bp
atggccaaaacgacgccgtcgccggcaattctcttcctcaccctcctcctcctgatcacgccgccgccctcccaatccctcccctactcccaataccggaccctcttctccctcgcccactcgctcatgacgcgcgtggccaacctccgcgcctcccgcggcgaccacgccggttcggacagggccaggaacatcgcggcgaaaatggagagcgggctgagcctcggcgtctgggggttcatgtggtcggcggggtgggactacgtcaagaactactcgtggagggagctgccgtacacggagatgtacggcgccgcgtcggaggcgggcgagttgatgaggtggctgggtgagttgactcggaaggaatcggactccgagcgagcggcatggatcggtcagaattaccaaaatgtcttcagggtctctacttcgctgctgcgtaggctcctccgagtgttcagtcagtcg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.78

Weight (kDa)

9.9

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 311
AccBSI CCGCTC 2 cut(s) 213, 386
AccII CGCG 6 cut(s) 133, 135, 150, 159, 197, 317
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 2 cut(s) 3, 138
AcuI CTGAAG 1 cut(s) 403
AcyI GRCGYC 3 cut(s) 14, 228, 312
AfaI GTAC 2 cut(s) 296, 308
AfiI CCNNNNNNNGG 1 cut(s) 96
AjnI CCWGG 1 cut(s) 185
AluBI AGCT 1 cut(s) 289
AluI AGCT 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 1 cut(s) 401
AoxI GGCC 3 cut(s) 3, 138, 183
ApeKI GCWGC 3 cut(s) 289, 436, 439
AspLEI GCGC 3 cut(s) 135, 152, 314
AspS9I GGNCC 2 cut(s) 99, 183
AsuHPI GGTGA 2 cut(s) 31, 362
AvaII GGWCC 1 cut(s) 99
BalI TGGCCA 2 cut(s) 5, 140
BanI GGYRCC 1 cut(s) 311
BauI CACGAG 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 409
BbvI GCAGC 3 cut(s) 276, 423, 426
BceAI ACGGC 2 cut(s) 277, 325
BciT130I CCWGG 1 cut(s) 187
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 1 cut(s) 430
BfoI RGCGCY 1 cut(s) 315
BisI GCNGC 9 cut(s) 62, 65, 160, 198, 290, 315, 387, 437, 440
BlpI GCTNAGC 1 cut(s) 218
BlsI GCNGC 9 cut(s) 63, 66, 161, 199, 291, 316, 388, 438, 441
Bme1390I CCNGG 1 cut(s) 187
Bme18I GGWCC 1 cut(s) 99
BmgT120I GGNCC 2 cut(s) 99, 183
BmiI GGNNCC 3 cut(s) 101, 313, 449
BmrFI CCNGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 409
Bpu1102I GCTNAGC 1 cut(s) 218
BsaHI GRCGYC 3 cut(s) 14, 228, 312
BsaI GGTCTC 1 cut(s) 430
BsaJI CCNNGG 2 cut(s) 157, 223
BsaWI WCCGGW 1 cut(s) 96
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse118I RCCGGY 2 cut(s) 22, 170
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 157, 223
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 209
BseRI GAGGAG 3 cut(s) 35, 38, 440
BseXI GCAGC 3 cut(s) 276, 423, 426
BseYI CCCAGC 1 cut(s) 346
Bsh1236I CGCG 6 cut(s) 133, 135, 150, 159, 197, 317
BshFI GGCC 3 cut(s) 5, 140, 185
BshNI GGYRCC 1 cut(s) 311
BsiSI CCGG 3 cut(s) 23, 97, 171
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 96
BsmAI GTCTC 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 3 cut(s) 5, 140, 185
Bso31I GGTCTC 1 cut(s) 430
Bsp143I GATC 2 cut(s) 54, 393
Bsp1720I GCTNAGC 1 cut(s) 218
BspANI GGCC 3 cut(s) 5, 140, 185
BspCNI CTCAG 1 cut(s) 210
BspFNI CGCG 6 cut(s) 133, 135, 150, 159, 197, 317
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 101, 313, 449
BspPI GGATC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 430
BsrBI CCGCTC 2 cut(s) 213, 386
BsrFI RCCGGY 2 cut(s) 22, 170
BssAI RCCGGY 2 cut(s) 22, 170
BssECI CCNNGG 2 cut(s) 157, 223
BssMI GATC 2 cut(s) 54, 393
BssNI GRCGYC 3 cut(s) 14, 228, 312
BssSI CACGAG 1 cut(s) 277
Bst2BI CACGAG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 187
Bst6I CTCTTC 2 cut(s) 38, 110
BstACI GRCGYC 3 cut(s) 14, 228, 312
BstC8I GCNNGC 4 cut(s) 24, 215, 328, 384
BstDEI CTNAG 1 cut(s) 218
BstDSI CCRYGG 1 cut(s) 157
BstFNI CGCG 6 cut(s) 133, 135, 150, 159, 197, 317
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 3 cut(s) 135, 152, 314
BstKTI GATC 2 cut(s) 57, 396
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 2 cut(s) 54, 393
BstMWI GCNNNNNNNGC 5 cut(s) 130, 156, 219, 323, 445
BstNI CCWGG 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 185
BstUI CGCG 6 cut(s) 133, 135, 150, 159, 197, 317
BstV1I GCAGC 3 cut(s) 276, 423, 426
BstV2I GAAGAC 1 cut(s) 409
BsuRI GGCC 3 cut(s) 5, 140, 185
BtgI CCRYGG 1 cut(s) 157
BtgZI GCGATG 1 cut(s) 178
Cac8I GCNNGC 4 cut(s) 24, 215, 328, 384
CciI TCATGA 1 cut(s) 126
CfoI GCGC 3 cut(s) 135, 152, 314
Cfr10I RCCGGY 2 cut(s) 22, 170
Cfr13I GGNCC 2 cut(s) 99, 183
Cfr42I CCGCGG 1 cut(s) 160
CseI GACGC 4 cut(s) 22, 139, 217, 306
Csp6I GTAC 2 cut(s) 295, 307
CviAII CATG 3 cut(s) 127, 241, 390
CviJI RGCY 8 cut(s) 5, 140, 185, 217, 222, 289, 346, 448
CviKI_1 RGCY 8 cut(s) 5, 140, 185, 217, 222, 289, 346, 448
CviQI GTAC 2 cut(s) 295, 307
DdeI CTNAG 1 cut(s) 218
DinI GGCGCC 1 cut(s) 313
DpnI GATC 2 cut(s) 56, 395
DpnII GATC 2 cut(s) 54, 393
EaeI YGGCCR 2 cut(s) 3, 138
Eam1104I CTCTTC 2 cut(s) 38, 110
EarI CTCTTC 2 cut(s) 38, 110
Eco31I GGTCTC 1 cut(s) 430
Eco47I GGWCC 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 185
EgeI GGCGCC 1 cut(s) 313
EheI GGCGCC 1 cut(s) 313
FaeI CATG 3 cut(s) 130, 244, 393
FaiI YATR 3 cut(s) 128, 242, 391
FaqI GGGAC 1 cut(s) 272
FatI CATG 3 cut(s) 126, 240, 389
FauI CCCGC 4 cut(s) 164, 206, 244, 319
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 9 cut(s) 62, 65, 160, 198, 290, 315, 387, 437, 440
Fsp4HI GCNGC 9 cut(s) 62, 65, 160, 198, 290, 315, 387, 437, 440
GlaI GCGC 3 cut(s) 134, 151, 313
GluI GCNGC 9 cut(s) 62, 65, 160, 198, 290, 315, 387, 437, 440
GsaI CCCAGC 1 cut(s) 350
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 3 cut(s) 5, 140, 185
HapII CCGG 3 cut(s) 23, 97, 171
HgaI GACGC 4 cut(s) 22, 139, 217, 306
HhaI GCGC 3 cut(s) 135, 152, 314
Hin1I GRCGYC 3 cut(s) 14, 228, 312
Hin1II CATG 3 cut(s) 130, 244, 393
Hin6I GCGC 3 cut(s) 133, 150, 312
HinP1I GCGC 3 cut(s) 133, 150, 312
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 3 cut(s) 358, 368, 374
HpaII CCGG 3 cut(s) 23, 97, 171
HphI GGTGA 2 cut(s) 31, 362
Hpy166II GTNNAC 2 cut(s) 297, 357
Hpy188I TCNGA 7 cut(s) 178, 322, 363, 373, 379, 402, 455
Hpy188III TCNNGA 3 cut(s) 52, 127, 268
Hpy8I GTNNAC 2 cut(s) 297, 357
Hpy99I CGWCG 3 cut(s) 16, 22, 322
HpyAV CCTTC 1 cut(s) 358
HpyCH4IV ACGT 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 156, 219, 323, 445
HpyF3I CTNAG 1 cut(s) 218
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 3 cut(s) 14, 228, 312
Hsp92II CATG 3 cut(s) 130, 244, 393
HspAI GCGC 3 cut(s) 133, 150, 312
KasI GGCGCC 1 cut(s) 311
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 54
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 2 cut(s) 54, 393
LmnI GCTCC 2 cut(s) 286, 453
Lsp1109I GCAGC 3 cut(s) 276, 423, 426
MaeII ACGT 1 cut(s) 264
MalI GATC 2 cut(s) 56, 395
MbiI CCGCTC 2 cut(s) 213, 386
MboI GATC 2 cut(s) 54, 393
MboII GAAGA 3 cut(s) 25, 97, 409
MlsI TGGCCA 2 cut(s) 5, 140
MluCI AATT 2 cut(s) 27, 403
MluNI TGGCCA 2 cut(s) 5, 140
Mly113I GGCGCC 1 cut(s) 312
MlyI GAGTC 2 cut(s) 352, 368
MmeI TCCRAC 1 cut(s) 300
Mox20I TGGCCA 2 cut(s) 5, 140
MroNI GCCGGC 1 cut(s) 22
MscI TGGCCA 2 cut(s) 5, 140
MslI CAYNNNNRTG 1 cut(s) 302
Msp20I TGGCCA 2 cut(s) 5, 140
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 3 cut(s) 23, 97, 171
MspR9I CCNGG 1 cut(s) 187
MvaI CCWGG 1 cut(s) 187
MvnI CGCG 6 cut(s) 133, 135, 150, 159, 197, 317
MwoI GCNNNNNNNGC 5 cut(s) 130, 156, 219, 323, 445
NaeI GCCGGC 1 cut(s) 24
NarI GGCGCC 1 cut(s) 312
NdeII GATC 2 cut(s) 54, 393
NgoMIV GCCGGC 1 cut(s) 22
NlaIII CATG 3 cut(s) 130, 244, 393
NlaIV GGNNCC 3 cut(s) 101, 313, 449
NmeAIII GCCGAG 1 cut(s) 204
PagI TCATGA 1 cut(s) 126
PdiI GCCGGC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 368
PflFI GACNNNGTC 2 cut(s) 16, 263
PkrI GCNGC 9 cut(s) 63, 66, 161, 199, 291, 316, 388, 438, 441
PleI GAGTC 2 cut(s) 352, 368
PluTI GGCGCC 1 cut(s) 315
PpsI GAGTC 2 cut(s) 352, 368
Psp6I CCWGG 1 cut(s) 185
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 3 cut(s) 101, 313, 449
PspPI GGNCC 2 cut(s) 99, 183
PsyI GACNNNGTC 2 cut(s) 16, 263
RsaI GTAC 2 cut(s) 296, 308
RsaNI GTAC 2 cut(s) 295, 307
RseI CAYNNNNRTG 1 cut(s) 302
SacII CCGCGG 1 cut(s) 160
SatI GCNGC 9 cut(s) 62, 65, 160, 198, 290, 315, 387, 437, 440
Sau3AI GATC 2 cut(s) 54, 393
Sau96I GGNCC 2 cut(s) 99, 183
SchI GAGTC 2 cut(s) 352, 368
ScrFI CCNGG 1 cut(s) 187
SetI ASST 4 cut(s) 147, 267, 291, 344
SfoI GGCGCC 1 cut(s) 313
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 302
Sse9I AATT 2 cut(s) 27, 403
SspDI GGCGCC 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 185
TaiI ACGT 1 cut(s) 267
TaqII GACCGA 1 cut(s) 386
TasI AATT 2 cut(s) 27, 403
TauI GCSGC 6 cut(s) 64, 67, 162, 200, 317, 389
TfiI GAWTC 1 cut(s) 368
TseI GCWGC 3 cut(s) 289, 436, 439
TspDTI ATGAA 1 cut(s) 229
TspGWI ACGGA 1 cut(s) 314
Tth111I GACNNNGTC 2 cut(s) 16, 263
VpaK11BI GGWCC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.