Rorug05G0002300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008430
Physical Location & Seq
Reverse (-)
153656 .. 155174
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0002300.1

Sequence Viewer

Length: 705 bp
ATGGGAAGAGAAGACGAAGAAGAAGAATACCAACAAGAAAGGGAAACTGATGATGAATCTTCAGCTTCAGAGGAAGAATCAGAATCGGAAGAGAAATGGAGAAAGCACTACTCATCCAGGCATAGGATATTGTTGGTCGGAGAAGGAGACTTCTCCTTCTCTGTGTCTTTAGCCACACACTTCGGATCAGCGCGCAACATGGTCGCCACCTCTCTCGATTCCCAGGAGAAGATATGTAGCAAGTACAGTAACGCAATAGCGAATGTGAGGGAGCTTGAAGAAAAGGGATGCCTAGTGCTCTTCGGAGTTGACGCCAAGACCATGAGTCAACACTACTTCCTCAGCACTCAGAGGTTCGACAGAATTGTCTACAACTTCCCTCATGTCGGCTTTCTCTTCCGGGAGGAAAGCTACTGCCAGATCCAGTTGAACAAGAGATTGGTCAAGGGTTTTCTCAGGAATGCTAAACTTCTTTTGAAAAAAGAGGGAGGGGAGATTCATATCTCTCACAAAGAAGGCCATCCTTACGACAAATGGGATTTAGTGAAGAAAGCACGCAAGATTGGAGGACTGGTTCTGACCCAATCTGTGCCCTTCTGCAAGGATGACTATCCTGGATATGACAACAAGAGAGCCCACGGTACCTTTTCCGATGCCTCTTTCCACCTTGGGGATTGCACTACTTACATGTTTAGCCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

26.95

Weight (kDa)

5.95

Isoelectric Point (pI)

56.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 44 - 211 6.8e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
Acc65I GGTACC 1 cut(s) 641
AccB1I GGYRCC 1 cut(s) 641
AccI GTMKAC 1 cut(s) 369
AccII CGCG 1 cut(s) 193
AclWI GGATC 2 cut(s) 193, 415
AcuI CTGAAG 2 cut(s) 45, 51
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 2 cut(s) 245, 643
AfiI CCNNNNNNNGG 3 cut(s) 123, 386, 670
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 3 cut(s) 278, 430, 478
AhdI GACNNNNNGTC 1 cut(s) 324
AjnI CCWGG 3 cut(s) 116, 222, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
AluBI AGCT 3 cut(s) 65, 274, 411
AluI AGCT 3 cut(s) 65, 274, 411
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 2 cut(s) 193, 415
AoxI GGCC 1 cut(s) 517
Asp718I GGTACC 1 cut(s) 641
AspLEI GCGC 2 cut(s) 193, 195
AsuC2I CCSGG 1 cut(s) 401
BaeGI GKGCMC 1 cut(s) 594
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 637
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 341
BccI CCATC 1 cut(s) 528
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 3 cut(s) 118, 224, 615
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 293
Bme1390I CCNGG 4 cut(s) 118, 224, 401, 615
BmeRI GACNNNNNGTC 1 cut(s) 324
BmiI GGNNCC 1 cut(s) 643
BmrFI CCNGG 4 cut(s) 118, 224, 401, 615
BmsI GCATC 2 cut(s) 278, 643
BpiI GAAGAC 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 401
BsaBI GATNNNNATC 2 cut(s) 500, 609
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 3 cut(s) 222, 637, 667
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 386, 670
Bse1I ACTGG 2 cut(s) 424, 576
Bse8I GATNNNNATC 2 cut(s) 500, 609
BseBI CCWGG 3 cut(s) 118, 224, 615
BseDI CCNNGG 3 cut(s) 222, 637, 667
BseGI GGATG 4 cut(s) 113, 293, 520, 610
BseJI GATNNNNATC 2 cut(s) 500, 609
BseLI CCNNNNNNNGG 3 cut(s) 123, 386, 670
BseMII CTCAG 3 cut(s) 355, 362, 469
BseNI ACTGG 2 cut(s) 424, 576
BsePI GCGCGC 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 594
Bsh1236I CGCG 1 cut(s) 193
BshFI GGCC 1 cut(s) 519
BshNI GGYRCC 1 cut(s) 641
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 1 cut(s) 400
BslI CCNNNNNNNGG 3 cut(s) 123, 386, 670
BsmAI GTCTC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 1 cut(s) 519
Bsp1286I GDGCHC 3 cut(s) 300, 594, 637
Bsp143I GATC 2 cut(s) 185, 420
BspANI GGCC 1 cut(s) 519
BspCNI CTCAG 3 cut(s) 354, 361, 468
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 643
BspPI GGATC 2 cut(s) 193, 415
BspQI GCTCTTC 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 641
BsrI ACTGG 2 cut(s) 424, 576
BssECI CCNNGG 3 cut(s) 222, 637, 667
BssHII GCGCGC 1 cut(s) 191
BssMI GATC 2 cut(s) 185, 420
BssNI GRCGYC 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 667
Bst2UI CCWGG 3 cut(s) 118, 224, 615
Bst4CI ACNGT 2 cut(s) 248, 641
Bst6I CTCTTC 3 cut(s) 84, 305, 401
BstACI GRCGYC 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 193, 556
BstDEI CTNAG 3 cut(s) 341, 348, 455
BstDSI CCRYGG 1 cut(s) 637
BstF5I GGATG 4 cut(s) 113, 293, 520, 610
BstFNI CGCG 1 cut(s) 193
BstHHI GCGC 2 cut(s) 193, 195
BstKTI GATC 2 cut(s) 188, 423
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 2 cut(s) 185, 420
BstNI CCWGG 3 cut(s) 118, 224, 615
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 4 cut(s) 116, 222, 399, 613
BstSLI GKGCMC 1 cut(s) 594
BstUI CGCG 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 420
BstYI RGATCY 1 cut(s) 420
BsuRI GGCC 1 cut(s) 519
BtgI CCRYGG 1 cut(s) 637
BtsCI GGATG 4 cut(s) 113, 293, 520, 610
Cac8I GCNNGC 2 cut(s) 193, 556
CfoI GCGC 2 cut(s) 193, 195
CseI GACGC 1 cut(s) 320
Csp6I GTAC 2 cut(s) 244, 642
CviAII CATG 4 cut(s) 199, 322, 383, 688
CviJI RGCY 8 cut(s) 65, 173, 274, 390, 411, 519, 635, 696
CviKI_1 RGCY 8 cut(s) 65, 173, 274, 390, 411, 519, 635, 696
CviQI GTAC 2 cut(s) 244, 642
DdeI CTNAG 3 cut(s) 341, 348, 455
DpnI GATC 2 cut(s) 187, 422
DpnII GATC 2 cut(s) 185, 420
DrdI GACNNNNNNGTC 1 cut(s) 365
DriI GACNNNNNGTC 1 cut(s) 324
DseDI GACNNNNNNGTC 1 cut(s) 365
Eam1104I CTCTTC 3 cut(s) 84, 305, 401
Eam1105I GACNNNNNGTC 1 cut(s) 324
EarI CTCTTC 3 cut(s) 84, 305, 401
Eco130I CCWWGG 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 637
Eco57I CTGAAG 2 cut(s) 45, 51
EcoRII CCWGG 3 cut(s) 116, 222, 613
EcoT14I CCWWGG 1 cut(s) 667
EcoT38I GRGCYC 1 cut(s) 637
ErhI CCWWGG 1 cut(s) 667
FaeI CATG 4 cut(s) 202, 325, 386, 691
FaiI YATR 8 cut(s) 123, 200, 235, 323, 384, 501, 621, 689
FatI CATG 4 cut(s) 198, 321, 382, 687
FblI GTMKAC 1 cut(s) 369
FokI GGATG 4 cut(s) 100, 300, 507, 617
FriOI GRGCYC 1 cut(s) 637
FspBI CTAG 1 cut(s) 293
GlaI GCGC 2 cut(s) 192, 194
HaeIII GGCC 1 cut(s) 519
HapII CCGG 1 cut(s) 400
HgaI GACGC 1 cut(s) 320
HhaI GCGC 2 cut(s) 193, 195
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 4 cut(s) 202, 325, 386, 691
Hin6I GCGC 2 cut(s) 191, 193
HinP1I GCGC 2 cut(s) 191, 193
HincII GTYRAC 2 cut(s) 310, 329
HindII GTYRAC 2 cut(s) 310, 329
HinfI GANTC 6 cut(s) 56, 77, 83, 218, 325, 496
HpaII CCGG 1 cut(s) 400
Hpy166II GTNNAC 3 cut(s) 310, 329, 370
Hpy188I TCNGA 9 cut(s) 70, 82, 88, 140, 185, 305, 351, 579, 652
Hpy188III TCNNGA 2 cut(s) 215, 457
Hpy8I GTNNAC 3 cut(s) 310, 329, 370
HpyAV CCTTC 4 cut(s) 137, 166, 509, 604
HpyCH4III ACNGT 2 cut(s) 248, 641
HpyCH4V TGCA 2 cut(s) 600, 678
HpyF3I CTNAG 3 cut(s) 341, 348, 455
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 4 cut(s) 202, 325, 386, 691
HspAI GCGC 2 cut(s) 191, 193
KpnI GGTACC 1 cut(s) 645
Kzo9I GATC 2 cut(s) 185, 420
LguI GCTCTTC 1 cut(s) 305
LmnI GCTCC 1 cut(s) 271
LweI GCATC 2 cut(s) 278, 643
MaeI CTAG 1 cut(s) 293
MaeIII GTNAC 1 cut(s) 248
MalI GATC 2 cut(s) 187, 422
MboI GATC 2 cut(s) 185, 420
MflI RGATCY 1 cut(s) 420
MhlI GDGCHC 3 cut(s) 300, 594, 637
MluCI AATT 1 cut(s) 363
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 1 cut(s) 118
MseI TTAA 1 cut(s) 703
MspI CCGG 1 cut(s) 400
MspR9I CCNGG 4 cut(s) 118, 224, 401, 615
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 3 cut(s) 118, 224, 615
MvnI CGCG 1 cut(s) 193
NciI CCSGG 1 cut(s) 401
NdeII GATC 2 cut(s) 185, 420
NlaIII CATG 4 cut(s) 202, 325, 386, 691
NlaIV GGNNCC 1 cut(s) 643
NspI RCATGY 1 cut(s) 691
PauI GCGCGC 1 cut(s) 191
PciI ACATGT 1 cut(s) 687
PciSI GCTCTTC 1 cut(s) 305
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 5 cut(s) 56, 77, 83, 218, 496
PfoI TCCNGGA 2 cut(s) 399, 613
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
PscI ACATGT 1 cut(s) 687
Psp6I CCWGG 3 cut(s) 116, 222, 613
PspGI CCWGG 3 cut(s) 116, 222, 613
PspN4I GGNNCC 1 cut(s) 643
PsuI RGATCY 1 cut(s) 420
PteI GCGCGC 1 cut(s) 191
RsaI GTAC 2 cut(s) 245, 643
RsaNI GTAC 2 cut(s) 244, 642
SapI GCTCTTC 1 cut(s) 305
SaqAI TTAA 1 cut(s) 703
Sau3AI GATC 2 cut(s) 185, 420
SchI GAGTC 1 cut(s) 334
ScrFI CCNGG 4 cut(s) 118, 224, 401, 615
SduI GDGCHC 3 cut(s) 300, 594, 637
SetI ASST 7 cut(s) 67, 212, 276, 356, 413, 647, 669
SfaNI GCATC 2 cut(s) 278, 643
Sse9I AATT 1 cut(s) 363
SspMI CTAG 1 cut(s) 293
StyD4I CCNGG 4 cut(s) 116, 222, 399, 613
StyI CCWWGG 1 cut(s) 667
TaaI ACNGT 2 cut(s) 248, 641
TaqI TCGA 2 cut(s) 216, 357
TasI AATT 1 cut(s) 363
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 5 cut(s) 56, 77, 83, 218, 496
Tru1I TTAA 1 cut(s) 703
Tru9I TTAA 1 cut(s) 703
TspDTI ATGAA 2 cut(s) 69, 488
XceI RCATGY 1 cut(s) 691
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.