FvH4_4g35390

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003085
Physical Location & Seq
Reverse (-)
32721729 .. 32724971
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35390.t1

Sequence Viewer

Length: 921 bp
ATGCCGTCTCACGGGGATCTGGACCGTCAGATCGAGCATCTGATGGAGTGCAAGACGTTGCCGGAGGCGGAGGTGAAGACGCTGTGCGAGCAAGCGAGGGCGATCCTGGTGGAGGAGTGGAACGTGCAACCGGTCAAGTGCCCGGTTACGGTGTGTGGAGACATACACGGCCAATTCTACGACCTCATTGAGCTGTTTCGGATAGGAGGCAACGCTCCTGATACTAATTACCTTTTCATGGGTGATTATGTAGATCGTGGGTATTATTCTGTGGAGACTGTCACACTTCTGGTGGCTCTGAAAGTTCGTTATAGAGATAGAATTACAATTCTGAGAGGAAATCATGAGAGTCGGCAAATTACTCAAGTGTATGGTTTCTACGATGAGTGTTTGAGAAAATACGGAAATGCCAATGTCTGGAAGTACTTTACTGACTTATTTGATTATCTTCCCCTCACAGCCCTTATTGAGAGTCAGATTTTCTGTTTGCACGGGGGCCTTTCACCATCTTTGGACACATTGGACAATATCCGAGCCTTGGACCGTATCCAGGAGGTTCCACATGAAGGACCAATGTGTGATCTCCTATGGTCTGATCCTGATGACCGCTGTGGTTGGGGAATATCTCCACGTGGTGCTGGTTATACATTTGGACAGGATATAGCTGCTCAGTTTAATCATACCAATGGACTCAGCCTGATTTCAAGAGCTCATCAGCTTGTCATGGAAGGATACAACTGGTGCCAGGAAAAGAACGTCGTGACTGTGTTTAGCGCTCCAAACTATTGTTACCGGTGTGGGAATATGGCTGCAATTTTGGAAATCGGGGAGAATATGGACCAGAATTTTCTACAATTTGACCCGGCTCCTCGTCAAATTGAGCCTGACAACACGCGGAAGACTCCAGATTATTTTTTGTAA

Protein Analysis

307

Amino Acids

35.05

Weight (kDa)

4.85

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 241 1.1e-46 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 741
AccB7I CCANNNNNTGG 1 cut(s) 417
AccII CGCG 1 cut(s) 895
AciI CCGC 3 cut(s) 68, 607, 895
AclWI GGATC 3 cut(s) 24, 97, 590
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 844
AcvI CACGTG 1 cut(s) 632
AdeI CACNNNGTG 1 cut(s) 635
AfaI GTAC 1 cut(s) 425
AfeI AGCGCT 1 cut(s) 775
AfiI CCNNNNNNNGG 8 cut(s) 11, 112, 148, 238, 417, 538, 550, 566
AgeI ACCGGT 2 cut(s) 130, 792
AgsI TTSAA 1 cut(s) 705
AjnI CCWGG 3 cut(s) 105, 549, 744
AluBI AGCT 4 cut(s) 193, 665, 710, 718
AluI AGCT 4 cut(s) 193, 665, 710, 718
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 3 cut(s) 12, 153, 269
AlwI GGATC 3 cut(s) 24, 97, 590
Aor51HI AGCGCT 1 cut(s) 775
AoxI GGCC 2 cut(s) 169, 496
ApeKI GCWGC 2 cut(s) 665, 809
ApoI RAATTY 1 cut(s) 844
AsiGI ACCGGT 2 cut(s) 130, 792
AspLEI GCGC 1 cut(s) 776
AspS9I GGNCC 5 cut(s) 22, 496, 541, 569, 838
AsuC2I CCSGG 2 cut(s) 143, 863
AsuHPI GGTGA 3 cut(s) 85, 254, 495
AvaII GGWCC 4 cut(s) 22, 541, 569, 838
BaeGI GKGCMC 1 cut(s) 143
BanI GGYRCC 1 cut(s) 741
BanII GRGCYC 1 cut(s) 712
BbrPI CACGTG 1 cut(s) 632
BbsI GAAGAC 2 cut(s) 83, 905
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 2 cut(s) 652, 796
BccI CCATC 2 cut(s) 37, 514
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 3 cut(s) 107, 551, 746
BciVI GTATCC 2 cut(s) 557, 725
BcnI CCSGG 2 cut(s) 143, 863
BcoDI GTCTC 3 cut(s) 12, 153, 269
BfoI RGCGCY 1 cut(s) 777
BfuI GTATCC 2 cut(s) 557, 725
BisI GCNGC 2 cut(s) 666, 810
BlsI GCNGC 2 cut(s) 667, 811
BmcAI AGTACT 1 cut(s) 425
Bme1390I CCNGG 5 cut(s) 107, 143, 551, 746, 863
Bme18I GGWCC 4 cut(s) 22, 541, 569, 838
BmgT120I GGNCC 5 cut(s) 22, 496, 541, 569, 838
BmiI GGNNCC 4 cut(s) 497, 558, 743, 867
BmrFI CCNGG 5 cut(s) 107, 143, 551, 746, 863
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 2 cut(s) 83, 905
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 1 cut(s) 348
BpuMI CCSGG 2 cut(s) 143, 863
BsaAI YACGTR 1 cut(s) 632
BsaJI CCNNGG 1 cut(s) 537
BsaWI WCCGGW 2 cut(s) 130, 792
Bsc4I CCNNNNNNNGG 8 cut(s) 11, 112, 148, 238, 417, 538, 550, 566
Bse118I RCCGGY 2 cut(s) 130, 792
Bse1I ACTGG 1 cut(s) 743
BseBI CCWGG 3 cut(s) 107, 551, 746
BseDI CCNNGG 1 cut(s) 537
BseLI CCNNNNNNNGG 8 cut(s) 11, 112, 148, 238, 417, 538, 550, 566
BseMII CTCAG 3 cut(s) 323, 683, 706
BseNI ACTGG 1 cut(s) 743
BseRI GAGGAG 2 cut(s) 128, 858
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 2 cut(s) 652, 796
Bsh1236I CGCG 1 cut(s) 895
BshFI GGCC 2 cut(s) 171, 498
BshNI GGYRCC 1 cut(s) 741
BshTI ACCGGT 2 cut(s) 130, 792
BsiHKAI GWGCWC 1 cut(s) 712
BsiSI CCGG 5 cut(s) 62, 131, 143, 793, 863
BslI CCNNNNNNNGG 8 cut(s) 11, 112, 148, 238, 417, 538, 550, 566
BsmAI GTCTC 3 cut(s) 12, 153, 269
BsmBI CGTCTC 1 cut(s) 12
BsnI GGCC 2 cut(s) 171, 498
Bsp1286I GDGCHC 2 cut(s) 143, 712
Bsp143I GATC 6 cut(s) 16, 30, 102, 253, 580, 595
BspACI CCGC 3 cut(s) 68, 607, 895
BspANI GGCC 2 cut(s) 171, 498
BspCNI CTCAG 3 cut(s) 324, 682, 705
BspFNI CGCG 1 cut(s) 895
BspHI TCATGA 1 cut(s) 343
BspLI GGNNCC 4 cut(s) 497, 558, 743, 867
BspPI GGATC 3 cut(s) 24, 97, 590
BspT107I GGYRCC 1 cut(s) 741
BsrFI RCCGGY 2 cut(s) 130, 792
BsrI ACTGG 1 cut(s) 743
BssAI RCCGGY 2 cut(s) 130, 792
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 6 cut(s) 16, 30, 102, 253, 580, 595
BssT1I CCWWGG 1 cut(s) 537
Bst2UI CCWGG 3 cut(s) 107, 551, 746
Bst4CI ACNGT 5 cut(s) 26, 151, 280, 545, 766
BstBAI YACGTR 1 cut(s) 632
BstC8I GCNNGC 2 cut(s) 89, 93
BstDEI CTNAG 3 cut(s) 332, 669, 692
BstENI CCTNNNNNAGG 1 cut(s) 110
BstFNI CGCG 1 cut(s) 895
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 1 cut(s) 776
BstKTI GATC 6 cut(s) 19, 33, 105, 256, 583, 598
BstMAI GTCTC 3 cut(s) 12, 153, 269
BstMBI GATC 6 cut(s) 16, 30, 102, 253, 580, 595
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 3 cut(s) 107, 551, 746
BstSCI CCNGG 5 cut(s) 105, 141, 549, 744, 861
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 1 cut(s) 895
BstV1I GCAGC 2 cut(s) 652, 796
BstV2I GAAGAC 2 cut(s) 83, 905
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuI GTATCC 2 cut(s) 557, 725
BsuRI GGCC 2 cut(s) 171, 498
Cac8I GCNNGC 2 cut(s) 89, 93
CciI TCATGA 1 cut(s) 343
CfoI GCGC 1 cut(s) 776
Cfr10I RCCGGY 2 cut(s) 130, 792
Cfr13I GGNCC 5 cut(s) 22, 496, 541, 569, 838
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 424
CspAI ACCGGT 2 cut(s) 130, 792
CviAII CATG 4 cut(s) 238, 344, 563, 724
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 3 cut(s) 332, 669, 692
DpnI GATC 6 cut(s) 18, 32, 104, 255, 582, 597
DpnII GATC 6 cut(s) 16, 30, 102, 253, 580, 595
DraIII CACNNNGTG 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 169
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 710
Eco130I CCWWGG 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 712
Eco47I GGWCC 4 cut(s) 22, 541, 569, 838
Eco47III AGCGCT 1 cut(s) 775
Eco53kI GAGCTC 1 cut(s) 710
Eco72I CACGTG 1 cut(s) 632
EcoICRI GAGCTC 1 cut(s) 710
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 3 cut(s) 105, 549, 744
EcoT14I CCWWGG 1 cut(s) 537
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 1 cut(s) 537
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 4 cut(s) 241, 347, 566, 727
FatI CATG 4 cut(s) 237, 343, 562, 723
Fnu4HI GCNGC 2 cut(s) 666, 810
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 2 cut(s) 666, 810
GlaI GCGC 1 cut(s) 775
GluI GCNGC 2 cut(s) 666, 810
GsuI CTGGAG 1 cut(s) 888
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 2 cut(s) 171, 498
HapII CCGG 5 cut(s) 62, 131, 143, 793, 863
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 776
Hin1II CATG 4 cut(s) 241, 347, 566, 727
Hin6I GCGC 1 cut(s) 774
HinP1I GCGC 1 cut(s) 774
HinfI GANTC 4 cut(s) 349, 472, 690, 901
HpaII CCGG 5 cut(s) 62, 131, 143, 793, 863
HphI GGTGA 3 cut(s) 85, 254, 495
Hpy188I TCNGA 8 cut(s) 30, 42, 201, 300, 333, 477, 533, 595
Hpy188III TCNNGA 8 cut(s) 20, 218, 344, 418, 599, 705, 760, 905
Hpy99I CGWCG 1 cut(s) 761
HpyAV CCTTC 2 cut(s) 560, 722
HpyCH4III ACNGT 5 cut(s) 26, 151, 280, 545, 766
HpyCH4IV ACGT 4 cut(s) 56, 123, 631, 756
HpyCH4V TGCA 4 cut(s) 51, 127, 490, 812
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 3 cut(s) 332, 669, 692
HpySE526I ACGT 4 cut(s) 56, 123, 631, 756
Hsp92II CATG 4 cut(s) 241, 347, 566, 727
HspAI GCGC 1 cut(s) 774
Kzo9I GATC 6 cut(s) 16, 30, 102, 253, 580, 595
LmnI GCTCC 3 cut(s) 220, 781, 871
Lsp1109I GCAGC 2 cut(s) 652, 796
LweI GCATC 1 cut(s) 46
MaeII ACGT 4 cut(s) 56, 123, 631, 756
MaeIII GTNAC 4 cut(s) 145, 280, 760, 788
MalI GATC 6 cut(s) 18, 32, 104, 255, 582, 597
MboI GATC 6 cut(s) 16, 30, 102, 253, 580, 595
MboII GAAGA 3 cut(s) 88, 440, 910
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 143, 712
MluCI AATT 9 cut(s) 173, 226, 321, 327, 357, 813, 844, 854, 876
MlyI GAGTC 4 cut(s) 358, 481, 684, 895
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 684
MspA1I CMGCKG 1 cut(s) 609
MspI CCGG 5 cut(s) 62, 131, 143, 793, 863
MspR9I CCNGG 5 cut(s) 107, 143, 551, 746, 863
MvaI CCWGG 3 cut(s) 107, 551, 746
MvnI CGCG 1 cut(s) 895
MwoI GCNNNNNNNGC 1 cut(s) 88
NciI CCSGG 2 cut(s) 143, 863
NdeII GATC 6 cut(s) 16, 30, 102, 253, 580, 595
NlaIII CATG 4 cut(s) 241, 347, 566, 727
NlaIV GGNNCC 4 cut(s) 497, 558, 743, 867
NmuCI GTSAC 2 cut(s) 280, 760
PagI TCATGA 1 cut(s) 343
PflMI CCANNNNNTGG 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 549
PinAI ACCGGT 2 cut(s) 130, 792
PkrI GCNGC 2 cut(s) 667, 811
PleI GAGTC 4 cut(s) 357, 480, 684, 895
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 4 cut(s) 357, 480, 684, 895
Ppu21I YACGTR 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 3 cut(s) 105, 549, 744
PspCI CACGTG 1 cut(s) 632
PspGI CCWGG 3 cut(s) 105, 549, 744
PspN4I GGNNCC 4 cut(s) 497, 558, 743, 867
PspPI GGNCC 5 cut(s) 22, 496, 541, 569, 838
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 1 cut(s) 684
SacI GAGCTC 1 cut(s) 712
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 2 cut(s) 666, 810
Sau3AI GATC 6 cut(s) 16, 30, 102, 253, 580, 595
Sau96I GGNCC 5 cut(s) 22, 496, 541, 569, 838
ScaI AGTACT 1 cut(s) 425
SchI GAGTC 4 cut(s) 358, 481, 684, 895
ScrFI CCNGG 5 cut(s) 107, 143, 551, 746, 863
SduI GDGCHC 2 cut(s) 143, 712
SfaNI GCATC 1 cut(s) 46
SinI GGWCC 4 cut(s) 22, 541, 569, 838
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
Sse9I AATT 9 cut(s) 173, 226, 321, 327, 357, 813, 844, 854, 876
SsiI CCGC 3 cut(s) 68, 607, 895
SstI GAGCTC 1 cut(s) 712
StyD4I CCNGG 5 cut(s) 105, 141, 549, 744, 861
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 5 cut(s) 26, 151, 280, 545, 766
TaiI ACGT 4 cut(s) 59, 126, 634, 759
TaqI TCGA 1 cut(s) 33
TasI AATT 9 cut(s) 173, 226, 321, 327, 357, 813, 844, 854, 876
TatI WGTACW 1 cut(s) 423
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseFI GTSAC 2 cut(s) 280, 760
TseI GCWGC 2 cut(s) 665, 809
Tsp45I GTSAC 2 cut(s) 280, 760
TspDTI ATGAA 2 cut(s) 226, 579
TspGWI ACGGA 1 cut(s) 417
Van91I CCANNNNNTGG 1 cut(s) 417
VpaK11BI GGWCC 4 cut(s) 22, 541, 569, 838
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 844
ZrmI AGTACT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.