MD13G1083600.v1.1

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003085
Physical Location & Seq
Forward (+)
5864560 .. 5867786
3227 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1083600.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCCGTCTCATTCGGATCTGGACCGTCAGATCGAGCATCTGATGGAGTGCAAGACGTTGCCGGAGGCGGAGGTGAAGACGCTGTGCGAGCAGGCGAGGGCGATCCTGGTGGAGGAGTGGAACGTGCAGCCGGTGAAGTGCCCCGTCACGGTGTGCGGAGATATACACGGCCAGTTCTACGACCTCATTGAGCTTTTTAGGATAGGAGGGAACGCTCCCGATACTAATTACCTTTTTATGGGTGATTATGTAGATCGTGGGTACTATTCTGTGGAGACTGTCACGCTTCTGGTCGCTCTGAAAGTCCGTTATAGAGATAGAATTACAATCCTCAGAGGAAATCACGAAAGCCGGCAAATTACTCAAGTGTATGGTTTTTATGACGAATGCTTGAGAAAGTATGGGAATGCCAATGTCTGGAAGTTCTTTACCGATTTATTTGATTATCTTCCCCTGACAGCCCTCATTGAGAGTCAGGTCTTCTGTTTGCATGGGGGTCTTTCACCATCTTTGGACACATTGGACAATATCCGAGCTTTGGACCGTATACAGGAGGTTCCACATGAAGGACCAATGTGTGATCTCTTGTGGTCTGATCCAGATGACCGCTGTGGGTGGGGAATATCTCCGCGGGGTGCTGGCTATACATTCGGGCAGGATATAGCTGCTCAGTTTAACCATACCAATGGACTGAGTCTGATTTCAAGAGCTCATCAGCTTGTCATGGAAGGATACAATTGGTGCCAGGAAAAGAATGTGGTGACCGTTTTCAGCGCTCCAAACTATTGCTATCGGTGTGGGAACATGGCCGCAATTTTGGAAATTGGCGAGAACATGGACCAGAATTTTCTGCAGTTCGACCCAGCCCCTCGTCAAATTGAGCCGGACAACACACGCAAGACTCCCGATTATTTTTTGTAA

Protein Analysis

307

Amino Acids

35.05

Weight (kDa)

4.85

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 241 4.2e-46 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 741
AccB7I CCANNNNNTGG 1 cut(s) 417
AccI GTMKAC 1 cut(s) 547
AccII CGCG 1 cut(s) 631
AciI CCGC 6 cut(s) 68, 156, 607, 629, 631, 810
AclWI GGATC 3 cut(s) 24, 97, 590
AcoI YGGCCR 2 cut(s) 169, 807
AcsI RAATTY 1 cut(s) 844
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 1 cut(s) 263
AfeI AGCGCT 1 cut(s) 775
AfiI CCNNNNNNNGG 7 cut(s) 112, 148, 238, 417, 538, 550, 566
AgsI TTSAA 1 cut(s) 705
AjnI CCWGG 2 cut(s) 105, 744
AluBI AGCT 5 cut(s) 193, 536, 665, 710, 718
AluI AGCT 5 cut(s) 193, 536, 665, 710, 718
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 2 cut(s) 12, 269
AlwI GGATC 3 cut(s) 24, 97, 590
Aor51HI AGCGCT 1 cut(s) 775
AoxI GGCC 2 cut(s) 169, 807
ApeKI GCWGC 2 cut(s) 127, 665
ApoI RAATTY 1 cut(s) 844
AspLEI GCGC 1 cut(s) 776
AspS9I GGNCC 4 cut(s) 22, 541, 569, 838
AsuHPI GGTGA 5 cut(s) 85, 145, 254, 495, 772
AvaII GGWCC 4 cut(s) 22, 541, 569, 838
BaeGI GKGCMC 1 cut(s) 143
BanI GGYRCC 1 cut(s) 741
BanII GRGCYC 1 cut(s) 712
BbsI GAAGAC 2 cut(s) 83, 472
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 2 cut(s) 139, 652
BccI CCATC 2 cut(s) 37, 514
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 2 cut(s) 107, 746
BciVI GTATCC 1 cut(s) 725
BcoDI GTCTC 2 cut(s) 12, 269
BfmI CTRYAG 1 cut(s) 851
BfoI RGCGCY 1 cut(s) 777
BfuI GTATCC 1 cut(s) 725
BisI GCNGC 3 cut(s) 128, 666, 810
BlsI GCNGC 3 cut(s) 129, 667, 811
Bme1390I CCNGG 2 cut(s) 107, 746
Bme18I GGWCC 4 cut(s) 22, 541, 569, 838
BmgT120I GGNCC 4 cut(s) 22, 541, 569, 838
BmiI GGNNCC 2 cut(s) 558, 743
BmrFI CCNGG 2 cut(s) 107, 746
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 2 cut(s) 83, 472
BpuEI CTTGAG 2 cut(s) 348, 412
BsaJI CCNNGG 1 cut(s) 629
Bsc4I CCNNNNNNNGG 7 cut(s) 112, 148, 238, 417, 538, 550, 566
Bse118I RCCGGY 2 cut(s) 130, 351
Bse1I ACTGG 1 cut(s) 172
BseBI CCWGG 2 cut(s) 107, 746
BseDI CCNNGG 1 cut(s) 629
BseLI CCNNNNNNNGG 7 cut(s) 112, 148, 238, 417, 538, 550, 566
BseMII CTCAG 3 cut(s) 346, 683, 683
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 2 cut(s) 139, 652
BseYI CCCAGC 1 cut(s) 862
BsgI GTGCAG 1 cut(s) 146
Bsh1236I CGCG 1 cut(s) 631
BshFI GGCC 2 cut(s) 171, 809
BshNI GGYRCC 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 712
BsiSI CCGG 4 cut(s) 62, 131, 352, 884
BslI CCNNNNNNNGG 7 cut(s) 112, 148, 238, 417, 538, 550, 566
BsmAI GTCTC 2 cut(s) 12, 269
BsmBI CGTCTC 1 cut(s) 12
BsmI GAATGC 2 cut(s) 392, 412
BsnI GGCC 2 cut(s) 171, 809
Bsp1286I GDGCHC 2 cut(s) 143, 712
Bsp143I GATC 6 cut(s) 16, 30, 102, 253, 580, 595
BspACI CCGC 6 cut(s) 68, 156, 607, 629, 631, 810
BspANI GGCC 2 cut(s) 171, 809
BspCNI CTCAG 3 cut(s) 345, 682, 684
BspFNI CGCG 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 558, 743
BspMAI CTGCAG 1 cut(s) 855
BspPI GGATC 3 cut(s) 24, 97, 590
BspT107I GGYRCC 1 cut(s) 741
BsrFI RCCGGY 2 cut(s) 130, 351
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 2 cut(s) 130, 351
BssECI CCNNGG 1 cut(s) 629
BssMI GATC 6 cut(s) 16, 30, 102, 253, 580, 595
BssNAI GTATAC 1 cut(s) 548
Bst1107I GTATAC 1 cut(s) 548
Bst2UI CCWGG 2 cut(s) 107, 746
Bst4CI ACNGT 5 cut(s) 26, 151, 280, 545, 766
BstC8I GCNNGC 4 cut(s) 89, 93, 353, 640
BstDEI CTNAG 3 cut(s) 332, 669, 692
BstDSI CCRYGG 1 cut(s) 629
BstEII GGTNACC 1 cut(s) 760
BstENI CCTNNNNNAGG 1 cut(s) 110
BstFNI CGCG 1 cut(s) 631
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 1 cut(s) 776
BstKTI GATC 6 cut(s) 19, 33, 105, 256, 583, 598
BstMAI GTCTC 2 cut(s) 12, 269
BstMBI GATC 6 cut(s) 16, 30, 102, 253, 580, 595
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 2 cut(s) 107, 746
BstPI GGTNACC 1 cut(s) 760
BstSCI CCNGG 2 cut(s) 105, 744
BstSFI CTRYAG 1 cut(s) 851
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 2 cut(s) 139, 652
BstV2I GAAGAC 2 cut(s) 83, 472
BstX2I RGATCY 1 cut(s) 16
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 1 cut(s) 16
BstZ17I GTATAC 1 cut(s) 548
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 2 cut(s) 171, 809
BtgI CCRYGG 1 cut(s) 629
Cac8I GCNNGC 4 cut(s) 89, 93, 353, 640
CfoI GCGC 1 cut(s) 776
Cfr10I RCCGGY 2 cut(s) 130, 351
Cfr13I GGNCC 4 cut(s) 22, 541, 569, 838
Cfr42I CCGCGG 1 cut(s) 632
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 262
CviAII CATG 5 cut(s) 491, 563, 724, 805, 835
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 332, 669, 692
DpnI GATC 6 cut(s) 18, 32, 104, 255, 582, 597
DpnII GATC 6 cut(s) 16, 30, 102, 253, 580, 595
DraIII CACNNNGTG 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 169, 807
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 710
Eco24I GRGCYC 1 cut(s) 712
Eco47I GGWCC 4 cut(s) 22, 541, 569, 838
Eco47III AGCGCT 1 cut(s) 775
Eco53kI GAGCTC 1 cut(s) 710
Eco91I GGTNACC 1 cut(s) 760
EcoICRI GAGCTC 1 cut(s) 710
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoO65I GGTNACC 1 cut(s) 760
EcoRII CCWGG 2 cut(s) 105, 744
EcoT38I GRGCYC 1 cut(s) 712
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 5 cut(s) 494, 566, 727, 808, 838
FatI CATG 5 cut(s) 490, 562, 723, 804, 834
FauI CCCGC 1 cut(s) 624
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 128, 666, 810
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 3 cut(s) 128, 666, 810
GlaI GCGC 1 cut(s) 775
GluI GCNGC 3 cut(s) 128, 666, 810
GsaI CCCAGC 1 cut(s) 866
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 2 cut(s) 171, 809
HapII CCGG 4 cut(s) 62, 131, 352, 884
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 776
Hin1II CATG 5 cut(s) 494, 566, 727, 808, 838
Hin6I GCGC 1 cut(s) 774
HinP1I GCGC 1 cut(s) 774
HinfI GANTC 3 cut(s) 472, 694, 901
HpaII CCGG 4 cut(s) 62, 131, 352, 884
HphI GGTGA 5 cut(s) 85, 145, 254, 495, 772
Hpy166II GTNNAC 1 cut(s) 548
Hpy188I TCNGA 8 cut(s) 16, 30, 42, 300, 335, 533, 595, 699
Hpy188III TCNNGA 7 cut(s) 20, 218, 344, 418, 599, 705, 905
Hpy8I GTNNAC 1 cut(s) 548
HpyAV CCTTC 2 cut(s) 560, 722
HpyCH4III ACNGT 5 cut(s) 26, 151, 280, 545, 766
HpyCH4IV ACGT 2 cut(s) 56, 123
HpyCH4V TGCA 4 cut(s) 51, 127, 490, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 3 cut(s) 332, 669, 692
HpySE526I ACGT 2 cut(s) 56, 123
Hsp92II CATG 5 cut(s) 494, 566, 727, 808, 838
HspAI GCGC 1 cut(s) 774
KroI GCCGGC 1 cut(s) 351
KroNI GCCGGC 1 cut(s) 353
KspI CCGCGG 1 cut(s) 632
Kzo9I GATC 6 cut(s) 16, 30, 102, 253, 580, 595
LmnI GCTCC 2 cut(s) 220, 781
Lsp1109I GCAGC 2 cut(s) 139, 652
LweI GCATC 1 cut(s) 46
MaeII ACGT 2 cut(s) 56, 123
MaeIII GTNAC 3 cut(s) 145, 280, 760
MalI GATC 6 cut(s) 18, 32, 104, 255, 582, 597
MboI GATC 6 cut(s) 16, 30, 102, 253, 580, 595
MboII GAAGA 3 cut(s) 88, 440, 472
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 143, 712
MluCI AATT 8 cut(s) 226, 321, 357, 736, 813, 822, 844, 876
MlyI GAGTC 3 cut(s) 481, 703, 895
MroNI GCCGGC 1 cut(s) 351
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 684
MspA1I CMGCKG 2 cut(s) 609, 631
MspI CCGG 4 cut(s) 62, 131, 352, 884
MspR9I CCNGG 2 cut(s) 107, 746
MunI CAATTG 1 cut(s) 736
Mva1269I GAATGC 2 cut(s) 392, 412
MvaI CCWGG 2 cut(s) 107, 746
MvnI CGCG 1 cut(s) 631
MwoI GCNNNNNNNGC 1 cut(s) 88
NaeI GCCGGC 1 cut(s) 353
NdeII GATC 6 cut(s) 16, 30, 102, 253, 580, 595
NgoMIV GCCGGC 1 cut(s) 351
NlaIII CATG 5 cut(s) 494, 566, 727, 808, 838
NlaIV GGNNCC 2 cut(s) 558, 743
NmuCI GTSAC 3 cut(s) 145, 280, 760
PctI GAATGC 2 cut(s) 392, 412
PdiI GCCGGC 1 cut(s) 353
PflFI GACNNNGTC 1 cut(s) 693
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 3 cut(s) 129, 667, 811
PleI GAGTC 3 cut(s) 480, 702, 895
PpsI GAGTC 3 cut(s) 480, 702, 895
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 2 cut(s) 105, 744
PspEI GGTNACC 1 cut(s) 760
PspFI CCCAGC 1 cut(s) 862
PspGI CCWGG 2 cut(s) 105, 744
PspN4I GGNNCC 2 cut(s) 558, 743
PspPI GGNCC 4 cut(s) 22, 541, 569, 838
PstI CTGCAG 1 cut(s) 855
PsuI RGATCY 1 cut(s) 16
PsyI GACNNNGTC 1 cut(s) 693
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 684
SacI GAGCTC 1 cut(s) 712
SacII CCGCGG 1 cut(s) 632
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 3 cut(s) 128, 666, 810
Sau3AI GATC 6 cut(s) 16, 30, 102, 253, 580, 595
Sau96I GGNCC 4 cut(s) 22, 541, 569, 838
SchI GAGTC 3 cut(s) 481, 703, 895
ScrFI CCNGG 2 cut(s) 107, 746
SduI GDGCHC 2 cut(s) 143, 712
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 851
Sfr303I CCGCGG 1 cut(s) 632
SgrBI CCGCGG 1 cut(s) 632
SinI GGWCC 4 cut(s) 22, 541, 569, 838
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 2 cut(s) 363, 391
SmoI CTYRAG 2 cut(s) 363, 391
Sse9I AATT 8 cut(s) 226, 321, 357, 736, 813, 822, 844, 876
SsiI CCGC 6 cut(s) 68, 156, 607, 629, 631, 810
SstI GAGCTC 1 cut(s) 712
StyD4I CCNGG 2 cut(s) 105, 744
TaaI ACNGT 5 cut(s) 26, 151, 280, 545, 766
TaiI ACGT 2 cut(s) 59, 126
TaqI TCGA 2 cut(s) 33, 858
TasI AATT 8 cut(s) 226, 321, 357, 736, 813, 822, 844, 876
TauI GCSGC 1 cut(s) 812
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseFI GTSAC 3 cut(s) 145, 280, 760
TseI GCWGC 2 cut(s) 127, 665
Tsp45I GTSAC 3 cut(s) 145, 280, 760
TspDTI ATGAA 1 cut(s) 579
TspGWI ACGGA 1 cut(s) 296
Tth111I GACNNNGTC 1 cut(s) 693
Van91I CCANNNNNTGG 1 cut(s) 417
VpaK11BI GGWCC 4 cut(s) 22, 541, 569, 838
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 844
XmiI GTMKAC 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.