MD06G1183800.v1.1

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003085
Physical Location & Seq
Reverse (-)
32220590 .. 32223741
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1183800.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCCACCGCACGCCGATCTGGACCGTCAGATTGAGCACCTGATGCAGTGCAAGCCTCTGCCGGAGGCGGAGGTGAAGACGCTGTGCGAGCAGGCGAGGACGATCTTGGTCGAGGAGTGGAACGTACAGCCGGTGAAGTGCCCGGTCACGGTTTGCGGAGACATCCATGGCCAGTTCCACGACCTCGTCGAGCTCTTTCGGATCGGCGGCAACGCCCCCGATACCAATTACCTCTTCATGGGCGACTATGTAGATCGAGGATACTACTCTGTGGAGACTGTCACACTCCTAGTGGCCCTGAAAGTACGTTACAGAGATAGAATTACAATTCTAAGAGGAAATCATGAGAGTCGGCAAATAACTCAAGTGTATGGTTTCTATGATGAGTGCTTGAGGAAATATGGAAATGCTAATGTCTGGAAGTTTTTCACTGACCTTTTTGATTATCTGCCGCTCACAGCCTTGATTGAGAGTCAGATCTTTTGCTTGCATGGTGGACTCTCACCTTCATTAGATACACTAGACAATATTCGTGCTCTGGACCGGATACAGGAGGTTCCTCATGAAGGTCCAATGTGTGATCTCTTGTGGTCTGACCCGGATGATCGATGTGGGTGGGGAATATCTCCTCGGGGTGCAGGGTATACATTTGGACAAGATATAGCAGCCCAGTTTAACCACACTAACGGGCTAAGTCTTATTTCTAGAGCTCACCAGCTTGTTATGGAAGGCTACAATTGGTCCCAGGAAAAGAATGTTGTGACGGTGTTTAGTGCTCCAAATTACTGCTATCGCTGTGGAAATATGGCTGCAATTCTTGAGATTGGAGAGAATATGGACCAGAATTTCCTTCAGTTTGACCCAGCTCCTCGTCAGGTTGAACCTGATACATCACGCAGAACTCCAGATTATTTTTTGTAA

Protein Analysis

307

Amino Acids

35.01

Weight (kDa)

4.96

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 241 3.2e-46 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 454
AccI GTMKAC 1 cut(s) 644
AciI CCGC 5 cut(s) 8, 68, 156, 207, 452
AclWI GGATC 1 cut(s) 209
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 1 cut(s) 836
AfaI GTAC 2 cut(s) 126, 306
AfiI CCNNNNNNNGG 4 cut(s) 148, 238, 550, 566
AgsI TTSAA 1 cut(s) 881
AjnI CCWGG 1 cut(s) 744
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 4 cut(s) 193, 710, 718, 866
AluI AGCT 4 cut(s) 193, 710, 718, 866
Alw21I GWGCWC 5 cut(s) 39, 195, 538, 712, 778
Alw26I GTCTC 2 cut(s) 153, 269
AlwI GGATC 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 2 cut(s) 169, 294
ApeKI GCWGC 2 cut(s) 665, 809
ApoI RAATTY 1 cut(s) 844
Asp700I GAANNNNTTC 1 cut(s) 425
AspS9I GGNCC 6 cut(s) 22, 295, 541, 569, 741, 838
AsuC2I CCSGG 2 cut(s) 143, 599
AsuHPI GGTGA 4 cut(s) 85, 145, 495, 704
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 5 cut(s) 22, 541, 569, 741, 838
BaeGI GKGCMC 1 cut(s) 143
BalI TGGCCA 1 cut(s) 171
BanII GRGCYC 2 cut(s) 195, 712
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 5 cut(s) 39, 195, 538, 712, 778
BbvI GCAGC 2 cut(s) 677, 796
BciT130I CCWGG 1 cut(s) 746
BciVI GTATCC 2 cut(s) 254, 540
BcnI CCSGG 2 cut(s) 143, 599
BcoDI GTCTC 2 cut(s) 153, 269
BfaI CTAG 3 cut(s) 290, 521, 705
BfuI GTATCC 2 cut(s) 254, 540
BglII AGATCT 1 cut(s) 477
BisI GCNGC 4 cut(s) 208, 452, 666, 810
BlsI GCNGC 4 cut(s) 209, 453, 667, 811
Bme1390I CCNGG 3 cut(s) 143, 599, 746
Bme18I GGWCC 5 cut(s) 22, 541, 569, 741, 838
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 6 cut(s) 22, 295, 541, 569, 741, 838
BmiI GGNNCC 2 cut(s) 558, 743
BmrFI CCNGG 3 cut(s) 143, 599, 746
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 1 cut(s) 33
BmuI ACTGGG 1 cut(s) 664
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 3 cut(s) 348, 412, 839
BpuMI CCSGG 2 cut(s) 143, 599
Bsa29I ATCGAT 1 cut(s) 607
BsaJI CCNNGG 3 cut(s) 166, 629, 744
BsaWI WCCGGW 1 cut(s) 543
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 238, 550, 566
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 172, 670
BseBI CCWGG 1 cut(s) 746
BseCI ATCGAT 1 cut(s) 607
BseDI CCNNGG 3 cut(s) 166, 629, 744
BseGI GGATG 2 cut(s) 162, 607
BseLI CCNNNNNNNGG 4 cut(s) 148, 238, 550, 566
BseNI ACTGG 2 cut(s) 172, 670
BseRI GAGGAG 3 cut(s) 128, 618, 858
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 2 cut(s) 677, 796
BseYI CCCAGC 1 cut(s) 862
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 2 cut(s) 171, 296
BshVI ATCGAT 1 cut(s) 607
BsiHKAI GWGCWC 5 cut(s) 39, 195, 538, 712, 778
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 5 cut(s) 62, 131, 143, 544, 599
BslFI GGGAC 1 cut(s) 727
BslI CCNNNNNNNGG 4 cut(s) 148, 238, 550, 566
BsmAI GTCTC 2 cut(s) 153, 269
BsmFI GGGAC 1 cut(s) 727
BsnI GGCC 2 cut(s) 171, 296
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 6 cut(s) 39, 143, 195, 538, 712, 778
Bsp143I GATC 7 cut(s) 16, 102, 201, 253, 477, 580, 604
Bsp19I CCATGG 1 cut(s) 166
BspACI CCGC 5 cut(s) 8, 68, 156, 207, 452
BspANI GGCC 2 cut(s) 171, 296
BspDI ATCGAT 1 cut(s) 607
BspHI TCATGA 2 cut(s) 343, 562
BspLI GGNNCC 2 cut(s) 558, 743
BspPI GGATC 1 cut(s) 209
BsrBI CCGCTC 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 172, 670
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 166, 629, 744
BssMI GATC 7 cut(s) 16, 102, 201, 253, 477, 580, 604
BssNAI GTATAC 1 cut(s) 645
BssT1I CCWWGG 1 cut(s) 166
Bst1107I GTATAC 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 4 cut(s) 26, 151, 280, 766
Bst6I CTCTTC 1 cut(s) 239
BstAPI GCANNNNNTGC 1 cut(s) 43
BstC8I GCNNGC 5 cut(s) 12, 53, 89, 93, 488
BstDEI CTNAG 2 cut(s) 332, 692
BstDSI CCRYGG 1 cut(s) 166
BstENI CCTNNNNNAGG 1 cut(s) 564
BstF5I GGATG 2 cut(s) 162, 607
BstKTI GATC 7 cut(s) 19, 105, 204, 256, 480, 583, 607
BstMAI GTCTC 2 cut(s) 153, 269
BstMBI GATC 7 cut(s) 16, 102, 201, 253, 477, 580, 604
BstMWI GCNNNNNNNGC 3 cut(s) 43, 52, 88
BstNI CCWGG 1 cut(s) 746
BstSCI CCNGG 3 cut(s) 141, 597, 744
BstSLI GKGCMC 1 cut(s) 143
BstV1I GCAGC 2 cut(s) 677, 796
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 1 cut(s) 477
BstYI RGATCY 1 cut(s) 477
BstZ17I GTATAC 1 cut(s) 645
Bsu15I ATCGAT 1 cut(s) 607
BsuI GTATCC 2 cut(s) 254, 540
BsuRI GGCC 2 cut(s) 171, 296
BsuTUI ATCGAT 1 cut(s) 607
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 2 cut(s) 162, 607
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 2 cut(s) 53, 429
Cac8I GCNNGC 5 cut(s) 12, 53, 89, 93, 488
CciI TCATGA 2 cut(s) 343, 562
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 6 cut(s) 22, 295, 541, 569, 741, 838
ClaI ATCGAT 1 cut(s) 607
CseI GACGC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 125, 305
CviAII CATG 5 cut(s) 167, 238, 344, 491, 563
CviQI GTAC 2 cut(s) 125, 305
DdeI CTNAG 2 cut(s) 332, 692
DpnI GATC 7 cut(s) 18, 104, 203, 255, 479, 582, 606
DpnII GATC 7 cut(s) 16, 102, 201, 253, 477, 580, 604
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 2 cut(s) 193, 710
Eco130I CCWWGG 1 cut(s) 166
Eco24I GRGCYC 2 cut(s) 195, 712
Eco47I GGWCC 5 cut(s) 22, 541, 569, 741, 838
Eco53kI GAGCTC 2 cut(s) 193, 710
Eco57I CTGAAG 1 cut(s) 836
Eco88I CYCGRG 1 cut(s) 630
EcoICRI GAGCTC 2 cut(s) 193, 710
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 744
EcoT14I CCWWGG 1 cut(s) 166
EcoT38I GRGCYC 2 cut(s) 195, 712
ErhI CCWWGG 1 cut(s) 166
FaeI CATG 5 cut(s) 170, 241, 347, 494, 566
FaqI GGGAC 1 cut(s) 727
FatI CATG 5 cut(s) 166, 237, 343, 490, 562
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 4 cut(s) 208, 452, 666, 810
FokI GGATG 2 cut(s) 149, 614
FriOI GRGCYC 2 cut(s) 195, 712
Fsp4HI GCNGC 4 cut(s) 208, 452, 666, 810
FspBI CTAG 3 cut(s) 290, 521, 705
GluI GCNGC 4 cut(s) 208, 452, 666, 810
GsaI CCCAGC 1 cut(s) 866
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 2 cut(s) 171, 296
HapII CCGG 5 cut(s) 62, 131, 143, 544, 599
HgaI GACGC 1 cut(s) 88
Hin1II CATG 5 cut(s) 170, 241, 347, 494, 566
HinfI GANTC 3 cut(s) 349, 472, 498
HpaII CCGG 5 cut(s) 62, 131, 143, 544, 599
HphI GGTGA 4 cut(s) 85, 145, 495, 704
Hpy166II GTNNAC 2 cut(s) 497, 645
Hpy188I TCNGA 4 cut(s) 30, 201, 477, 595
Hpy188III TCNNGA 8 cut(s) 20, 344, 418, 539, 563, 705, 818, 905
Hpy8I GTNNAC 2 cut(s) 497, 645
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 4 cut(s) 516, 560, 722, 860
HpyCH4III ACNGT 4 cut(s) 26, 151, 280, 766
HpyCH4IV ACGT 2 cut(s) 123, 307
HpyCH4V TGCA 5 cut(s) 46, 51, 490, 638, 812
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 52, 88
HpyF3I CTNAG 2 cut(s) 332, 692
HpySE526I ACGT 2 cut(s) 123, 307
Hsp92II CATG 5 cut(s) 170, 241, 347, 494, 566
Kzo9I GATC 7 cut(s) 16, 102, 201, 253, 477, 580, 604
LmnI GCTCC 2 cut(s) 781, 871
Lsp1109I GCAGC 2 cut(s) 677, 796
LweI GCATC 1 cut(s) 33
MaeI CTAG 3 cut(s) 290, 521, 705
MaeII ACGT 2 cut(s) 123, 307
MaeIII GTNAC 4 cut(s) 145, 280, 308, 760
MalI GATC 7 cut(s) 18, 104, 203, 255, 479, 582, 606
MbiI CCGCTC 1 cut(s) 454
MboI GATC 7 cut(s) 16, 102, 201, 253, 477, 580, 604
MboII GAAGA 2 cut(s) 88, 226
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 6 cut(s) 39, 143, 195, 538, 712, 778
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 7 cut(s) 226, 321, 327, 736, 781, 813, 844
MluNI TGGCCA 1 cut(s) 171
MlyI GAGTC 3 cut(s) 358, 481, 492
Mox20I TGGCCA 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 425
MscI TGGCCA 1 cut(s) 171
MseI TTAA 1 cut(s) 675
Msp20I TGGCCA 1 cut(s) 171
MspI CCGG 5 cut(s) 62, 131, 143, 544, 599
MspR9I CCNGG 3 cut(s) 143, 599, 746
MunI CAATTG 1 cut(s) 736
MvaI CCWGG 1 cut(s) 746
MwoI GCNNNNNNNGC 3 cut(s) 43, 52, 88
NciI CCSGG 2 cut(s) 143, 599
NcoI CCATGG 1 cut(s) 166
NdeII GATC 7 cut(s) 16, 102, 201, 253, 477, 580, 604
NlaIII CATG 5 cut(s) 170, 241, 347, 494, 566
NlaIV GGNNCC 2 cut(s) 558, 743
NmuCI GTSAC 3 cut(s) 145, 280, 760
PagI TCATGA 2 cut(s) 343, 562
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 425
PflFI GACNNNGTC 1 cut(s) 185
PkrI GCNGC 4 cut(s) 209, 453, 667, 811
PleI GAGTC 3 cut(s) 357, 480, 492
PpsI GAGTC 3 cut(s) 357, 480, 492
Psp124BI GAGCTC 2 cut(s) 195, 712
Psp6I CCWGG 1 cut(s) 744
PspFI CCCAGC 1 cut(s) 862
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 2 cut(s) 558, 743
PspPI GGNCC 6 cut(s) 22, 295, 541, 569, 741, 838
PsuI RGATCY 1 cut(s) 477
PsyI GACNNNGTC 1 cut(s) 185
RsaI GTAC 2 cut(s) 126, 306
RsaNI GTAC 2 cut(s) 125, 305
SacI GAGCTC 2 cut(s) 195, 712
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 4 cut(s) 208, 452, 666, 810
Sau3AI GATC 7 cut(s) 16, 102, 201, 253, 477, 580, 604
Sau96I GGNCC 6 cut(s) 22, 295, 541, 569, 741, 838
SchI GAGTC 3 cut(s) 358, 481, 492
ScrFI CCNGG 3 cut(s) 143, 599, 746
SduI GDGCHC 6 cut(s) 39, 143, 195, 538, 712, 778
SfaNI GCATC 1 cut(s) 33
SinI GGWCC 5 cut(s) 22, 541, 569, 741, 838
SmlI CTYRAG 3 cut(s) 363, 391, 818
SmoI CTYRAG 3 cut(s) 363, 391, 818
Sse9I AATT 7 cut(s) 226, 321, 327, 736, 781, 813, 844
SsiI CCGC 5 cut(s) 8, 68, 156, 207, 452
SspI AATATT 1 cut(s) 529
SspMI CTAG 3 cut(s) 290, 521, 705
SstI GAGCTC 2 cut(s) 195, 712
StyD4I CCNGG 3 cut(s) 141, 597, 744
StyI CCWWGG 1 cut(s) 166
TaaI ACNGT 4 cut(s) 26, 151, 280, 766
TaiI ACGT 2 cut(s) 126, 310
TaqI TCGA 4 cut(s) 111, 189, 256, 607
TasI AATT 7 cut(s) 226, 321, 327, 736, 781, 813, 844
TauI GCSGC 2 cut(s) 210, 454
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TscAI CASTG 2 cut(s) 53, 436
TseFI GTSAC 3 cut(s) 145, 280, 760
TseI GCWGC 2 cut(s) 665, 809
Tsp45I GTSAC 3 cut(s) 145, 280, 760
TspDTI ATGAA 3 cut(s) 226, 498, 579
TspRI CASTG 2 cut(s) 53, 436
Tth111I GACNNNGTC 1 cut(s) 185
VpaK11BI GGWCC 5 cut(s) 22, 541, 569, 741, 838
XagI CCTNNNNNAGG 1 cut(s) 564
XapI RAATTY 1 cut(s) 844
XbaI TCTAGA 1 cut(s) 704
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 3 cut(s) 290, 521, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.