Rh7CG032300

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0003085
Physical Location & Seq
Reverse (-)
2237018 .. 2239566
2549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG032300.1

Sequence Viewer

Length: 921 bp
ATGCCGCCGAAAGCCGATCTGGACCGTCAGATCGACCACCTGATGCAGTGCAAGCCTCTGTCGGAGGCGGAGGTGAAGACGCTGTGCGAGCAAGCGAGGACGATCCTCGTCGAGGAGTGGAATGTGCAGCCGGTCAAGTGTCCGGTCACCGTCTGCGGCGACATCCACGGCCAGTTTCATGACCTGGTTGAGCTCTTTCGGATCGGTGGCAGTGCTCCCGATACCAATTATCTCTTCATGGGTGACTATGTAGATCGAGGATACTACTCAGTGGAGACTGTCACACTTCTAGTGGCCTTGAAAGTACGTTACAGAGATAGAATTACAATTCTCAGAGGAAATCATGAGAGTCGGCAAATTACTCAAGTGTATGGGTTTTATGATGAGTGTTTGAGGAAATATGGAAATGCTAATGTCTGGAAGCATTTCACCGACCTTTTTGATTATCTGCCATTGACGGCTTTAATCGAGAGTCAGATCTTCTGCTTGCATGGTGGACTATCCCCCTCTTTGGATACATTGGACAATATTCGGGCTCTTGATCGCATACAGGAGGTTCCTCATGAGGGCCCAATGTGTGATCTCTTGTGGTCTGATCCGGATGATCGATGTGGTTGGGGAATATCTCCTCGAGGTGCAGGGTATACTTTTGGTCAAGATATAGCAGCTCAGTTTAACCATACTAATGGGCTGAGTCTTATTTCTAGAGCTCACCAGCTTGTTATGGAAGGCTACAATTGGTGCCAGGAAAAGAACGTTGTAACGGTATTTAGTGCTCCAAATTACTGCTATCGATGTGGCAATATGGCTGCAATCCTCGAGATTGGAGAGAATATGGAGCAGAATTTCCTTCAATTTGACCCAGCACCTCGTCAGGTTGAACCCGATACAACACGAAGAACTCCAGACTATTTTTTGTAA

Protein Analysis

306

Amino Acids

34.98

Weight (kDa)

5.03

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 241 2.4e-46 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 630
AccB1I GGYRCC 1 cut(s) 741
AccI GTMKAC 1 cut(s) 644
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 3 cut(s) 5, 68, 156
AclI AACGTT 1 cut(s) 756
AclWI GGATC 3 cut(s) 97, 209, 590
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 844
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 3 cut(s) 112, 511, 566
AgsI TTSAA 3 cut(s) 301, 854, 881
AjnI CCWGG 2 cut(s) 183, 744
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 4 cut(s) 193, 668, 710, 718
AluI AGCT 4 cut(s) 193, 668, 710, 718
Alw21I GWGCWC 4 cut(s) 195, 217, 712, 778
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 97, 209, 590
Ama87I CYCGRG 2 cut(s) 630, 818
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 3 cut(s) 169, 294, 568
ApaI GGGCCC 1 cut(s) 572
ApeKI GCWGC 3 cut(s) 127, 665, 809
ApoI RAATTY 1 cut(s) 844
Asp700I GAANNNNTTC 1 cut(s) 425
AspS9I GGNCC 3 cut(s) 22, 568, 569
AsuHPI GGTGA 5 cut(s) 85, 139, 254, 421, 704
AvaI CYCGRG 2 cut(s) 630, 818
AvaII GGWCC 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 572
BanI GGYRCC 1 cut(s) 741
BanII GRGCYC 4 cut(s) 195, 538, 572, 712
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 4 cut(s) 195, 217, 712, 778
BbvI GCAGC 3 cut(s) 139, 677, 796
BceAI ACGGC 2 cut(s) 184, 474
BciT130I CCWGG 2 cut(s) 185, 746
BciVI GTATCC 2 cut(s) 254, 508
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 2 cut(s) 290, 705
BfuI GTATCC 2 cut(s) 254, 508
BglII AGATCT 1 cut(s) 477
BisI GCNGC 5 cut(s) 5, 128, 157, 666, 810
BlsI GCNGC 5 cut(s) 6, 129, 158, 667, 811
Bme1390I CCNGG 2 cut(s) 185, 746
Bme18I GGWCC 1 cut(s) 22
BmeT110I CYCGRG 2 cut(s) 630, 818
BmgT120I GGNCC 3 cut(s) 22, 568, 569
BmiI GGNNCC 3 cut(s) 558, 570, 743
BmrFI CCNGG 2 cut(s) 185, 746
BmsI GCATC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 1 cut(s) 348
Bsa29I ATCGAT 2 cut(s) 607, 793
BsaJI CCNNGG 1 cut(s) 166
BsaWI WCCGGW 2 cut(s) 142, 598
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 511, 566
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 172
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 2 cut(s) 185, 746
BseCI ATCGAT 2 cut(s) 607, 793
BseDI CCNNGG 1 cut(s) 166
BseGI GGATG 2 cut(s) 162, 607
BseLI CCNNNNNNNGG 3 cut(s) 112, 511, 566
BseMII CTCAG 4 cut(s) 282, 346, 683, 683
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 2 cut(s) 128, 618
BseSI GKGCMC 1 cut(s) 572
BseXI GCAGC 3 cut(s) 139, 677, 796
BseYI CCCAGC 1 cut(s) 862
BsgI GTGCAG 2 cut(s) 146, 657
BshFI GGCC 3 cut(s) 171, 296, 570
BshNI GGYRCC 1 cut(s) 741
BshVI ATCGAT 2 cut(s) 607, 793
BsiHKAI GWGCWC 4 cut(s) 195, 217, 712, 778
BsiHKCI CYCGRG 2 cut(s) 630, 818
BsiSI CCGG 3 cut(s) 131, 143, 599
BslI CCNNNNNNNGG 3 cut(s) 112, 511, 566
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 3 cut(s) 171, 296, 570
BsoBI CYCGRG 2 cut(s) 630, 818
Bsp120I GGGCCC 1 cut(s) 568
Bsp1286I GDGCHC 6 cut(s) 195, 217, 538, 572, 712, 778
Bsp13I TCCGGA 1 cut(s) 598
BspACI CCGC 3 cut(s) 5, 68, 156
BspANI GGCC 3 cut(s) 171, 296, 570
BspCNI CTCAG 4 cut(s) 281, 345, 682, 684
BspDI ATCGAT 2 cut(s) 607, 793
BspEI TCCGGA 1 cut(s) 598
BspHI TCATGA 3 cut(s) 178, 343, 562
BspLI GGNNCC 3 cut(s) 558, 570, 743
BspPI GGATC 3 cut(s) 97, 209, 590
BspT107I GGYRCC 1 cut(s) 741
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 166
BssNAI GTATAC 1 cut(s) 645
Bst1107I GTATAC 1 cut(s) 645
Bst2UI CCWGG 2 cut(s) 185, 746
Bst4CI ACNGT 4 cut(s) 26, 151, 280, 766
Bst6I CTCTTC 1 cut(s) 239
BstC8I GCNNGC 4 cut(s) 53, 89, 93, 488
BstDEI CTNAG 4 cut(s) 268, 332, 669, 692
BstDSI CCRYGG 1 cut(s) 166
BstEII GGTNACC 1 cut(s) 145
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 2 cut(s) 162, 607
BstMAI GTCTC 1 cut(s) 269
BstMWI GCNNNNNNNGC 2 cut(s) 52, 88
BstNI CCWGG 2 cut(s) 185, 746
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 183, 744
BstSLI GKGCMC 1 cut(s) 572
BstV1I GCAGC 3 cut(s) 139, 677, 796
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 1 cut(s) 477
BstZ17I GTATAC 1 cut(s) 645
Bsu15I ATCGAT 2 cut(s) 607, 793
BsuI GTATCC 2 cut(s) 254, 508
BsuRI GGCC 3 cut(s) 171, 296, 570
BsuTUI ATCGAT 2 cut(s) 607, 793
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 2 cut(s) 162, 607
BtsI GCAGTG 2 cut(s) 53, 217
BtsIMutI CAGTG 3 cut(s) 53, 217, 276
Cac8I GCNNGC 4 cut(s) 53, 89, 93, 488
CciI TCATGA 3 cut(s) 178, 343, 562
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 3 cut(s) 22, 568, 569
ClaI ATCGAT 2 cut(s) 607, 793
CseI GACGC 1 cut(s) 88
CsiI ACCWGGT 1 cut(s) 183
Csp6I GTAC 1 cut(s) 305
CviAII CATG 5 cut(s) 179, 238, 344, 491, 563
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 4 cut(s) 268, 332, 669, 692
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 2 cut(s) 193, 710
Eco24I GRGCYC 4 cut(s) 195, 538, 572, 712
Eco47I GGWCC 1 cut(s) 22
Eco53kI GAGCTC 2 cut(s) 193, 710
Eco88I CYCGRG 2 cut(s) 630, 818
Eco91I GGTNACC 1 cut(s) 145
EcoICRI GAGCTC 2 cut(s) 193, 710
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 568
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 183, 744
EcoT38I GRGCYC 4 cut(s) 195, 538, 572, 712
FaeI CATG 5 cut(s) 182, 241, 347, 494, 566
FatI CATG 5 cut(s) 178, 237, 343, 490, 562
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 5 cut(s) 5, 128, 157, 666, 810
FokI GGATG 2 cut(s) 149, 614
FriOI GRGCYC 4 cut(s) 195, 538, 572, 712
Fsp4HI GCNGC 5 cut(s) 5, 128, 157, 666, 810
FspBI CTAG 2 cut(s) 290, 705
GluI GCNGC 5 cut(s) 5, 128, 157, 666, 810
GsaI CCCAGC 1 cut(s) 866
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 3 cut(s) 171, 296, 570
HapII CCGG 3 cut(s) 131, 143, 599
HgaI GACGC 1 cut(s) 88
Hin1II CATG 5 cut(s) 182, 241, 347, 494, 566
HinfI GANTC 3 cut(s) 349, 472, 694
HpaII CCGG 3 cut(s) 131, 143, 599
HphI GGTGA 5 cut(s) 85, 139, 254, 421, 704
Hpy166II GTNNAC 2 cut(s) 497, 645
Hpy188I TCNGA 6 cut(s) 30, 64, 201, 335, 477, 595
Hpy8I GTNNAC 2 cut(s) 497, 645
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 2 cut(s) 722, 860
HpyCH4III ACNGT 4 cut(s) 26, 151, 280, 766
HpyCH4IV ACGT 2 cut(s) 307, 756
HpyCH4V TGCA 6 cut(s) 46, 51, 127, 490, 638, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 88
HpyF3I CTNAG 4 cut(s) 268, 332, 669, 692
HpySE526I ACGT 2 cut(s) 307, 756
Hsp92II CATG 5 cut(s) 182, 241, 347, 494, 566
Kpn2I TCCGGA 1 cut(s) 598
LmnI GCTCC 3 cut(s) 220, 781, 838
Lsp1109I GCAGC 3 cut(s) 139, 677, 796
LweI GCATC 1 cut(s) 33
MabI ACCWGGT 1 cut(s) 183
MaeI CTAG 2 cut(s) 290, 705
MaeII ACGT 2 cut(s) 307, 756
MaeIII GTNAC 5 cut(s) 145, 242, 280, 308, 760
MboII GAAGA 4 cut(s) 88, 226, 472, 909
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 6 cut(s) 195, 217, 538, 572, 712, 778
MluCI AATT 8 cut(s) 226, 321, 327, 357, 736, 781, 844, 854
MlyI GAGTC 3 cut(s) 358, 481, 703
MmeI TCCRAC 1 cut(s) 42
MroI TCCGGA 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 2 cut(s) 464, 675
MslI CAYNNNNRTG 1 cut(s) 684
MspI CCGG 3 cut(s) 131, 143, 599
MspR9I CCNGG 2 cut(s) 185, 746
MunI CAATTG 1 cut(s) 736
MvaI CCWGG 2 cut(s) 185, 746
MwoI GCNNNNNNNGC 2 cut(s) 52, 88
NlaIII CATG 5 cut(s) 182, 241, 347, 494, 566
NlaIV GGNNCC 3 cut(s) 558, 570, 743
NmuCI GTSAC 3 cut(s) 145, 242, 280
PaeR7I CTCGAG 2 cut(s) 630, 818
PagI TCATGA 3 cut(s) 178, 343, 562
PdmI GAANNNNTTC 1 cut(s) 425
PkrI GCNGC 5 cut(s) 6, 129, 158, 667, 811
PleI GAGTC 3 cut(s) 357, 480, 702
PpsI GAGTC 3 cut(s) 357, 480, 702
Psp124BI GAGCTC 2 cut(s) 195, 712
Psp1406I AACGTT 1 cut(s) 756
Psp6I CCWGG 2 cut(s) 183, 744
PspEI GGTNACC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 862
PspGI CCWGG 2 cut(s) 183, 744
PspN4I GGNNCC 3 cut(s) 558, 570, 743
PspOMI GGGCCC 1 cut(s) 568
PspPI GGNCC 3 cut(s) 22, 568, 569
PspXI VCTCGAGB 1 cut(s) 630
PsuI RGATCY 1 cut(s) 477
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 684
SacI GAGCTC 2 cut(s) 195, 712
SaqAI TTAA 2 cut(s) 464, 675
SatI GCNGC 5 cut(s) 5, 128, 157, 666, 810
Sau96I GGNCC 3 cut(s) 22, 568, 569
SchI GAGTC 3 cut(s) 358, 481, 703
ScrFI CCNGG 2 cut(s) 185, 746
SduI GDGCHC 6 cut(s) 195, 217, 538, 572, 712, 778
SexAI ACCWGGT 1 cut(s) 183
SfaNI GCATC 1 cut(s) 33
Sfr274I CTCGAG 2 cut(s) 630, 818
SinI GGWCC 1 cut(s) 22
SlaI CTCGAG 2 cut(s) 630, 818
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 3 cut(s) 363, 630, 818
SmoI CTYRAG 3 cut(s) 363, 630, 818
Sse9I AATT 8 cut(s) 226, 321, 327, 357, 736, 781, 844, 854
SsiI CCGC 3 cut(s) 5, 68, 156
SspI AATATT 1 cut(s) 529
SspMI CTAG 2 cut(s) 290, 705
SstI GAGCTC 2 cut(s) 195, 712
StyD4I CCNGG 2 cut(s) 183, 744
TaaI ACNGT 4 cut(s) 26, 151, 280, 766
TaiI ACGT 2 cut(s) 310, 759
TaqI TCGA 8 cut(s) 33, 111, 256, 468, 607, 631, 793, 819
TasI AATT 8 cut(s) 226, 321, 327, 357, 736, 781, 844, 854
TauI GCSGC 2 cut(s) 7, 159
Tru1I TTAA 2 cut(s) 464, 675
Tru9I TTAA 2 cut(s) 464, 675
TscAI CASTG 3 cut(s) 53, 217, 276
TseFI GTSAC 3 cut(s) 145, 242, 280
TseI GCWGC 3 cut(s) 127, 665, 809
Tsp45I GTSAC 3 cut(s) 145, 242, 280
TspDTI ATGAA 2 cut(s) 167, 226
TspRI CASTG 3 cut(s) 53, 217, 276
VpaK11BI GGWCC 1 cut(s) 22
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 844
XbaI TCTAGA 1 cut(s) 704
XhoI CTCGAG 2 cut(s) 630, 818
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 2 cut(s) 290, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.