FvH4_5g16000

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003085
Physical Location & Seq
Forward (+)
9080210 .. 9083780
3571 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16000.t1

Sequence Viewer

Length: 921 bp
ATGCCGCCGAACGCCGATCTGGACCGCCAGATCGACCACCTGATGCAGTGCAAGCCTCTGACGGAGGCGGAGGTCAAGACGCTGTGCGAGCAGGCGAGGACGATCCTCGTCGAGGAGTGGAATGTCCAGCCGGTCAAGTGTCCGGTCACCGTCTGCGGCGACATCCACGGCCAGTTTCACGACCTTGTTGAGCTTTTCCGGATCGGCGGCAGCGCTCCCGATACTAACTACCTCTTCATGGGCGACTATGTAGATCGAGGATACTACTCCGTGGAGACTGTCACACTTCTAGTGGCCCTGAAAGTACGTTACAGAGATAGAATTACAATTCTCAGAGGAAATCATGAGAGTCGGCAAATTACTCAAGTGTATGGGTTTTATGATGAGTGTTTGAGGAAATATGGAAATGCCAATGTCTGGAAGCATTTCACTGACCTTTTTGATTATCTGCCACTGACAGCCTTAATTGAGAGTCAGATCTTCTGCTTGCATGGTGGACTTTCCCCCTCTTTGGATACCTTGGACAATATTCGGGCTCTTGATCGCATACAGGAGGTTCCTCATGAGGGCCCAATGTGCGATCTCTTGTGGTCGGACCCGGATGATCGATGCGGTTGGGGAATATCTCCTCGAGGTGCAGGGTATACTTTTGGTCAAGATATAGCAGCACAGTTTAACCATACTAATGGGCTGAGTCTTATTTCTAGAGCTCACCAGCTTGTTATGGAAGGCTACAATTGGTGCCAGGAAAAGAATGTTGTAACGGTATTTAGTGCTCCAAATTACTGCTATCGATGTGGAAATATGGCTGCAATCCTTGAGATTGGAGAGAACATGGAGCAGAATTTCCTTCAATTTGACCCAGCACCTCGCCAAGTTGAACCCGATACAACACGAAGAACTCCAGACTATTTTTTGTGA

Protein Analysis

307

Amino Acids

34.98

Weight (kDa)

4.96

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 241 2.4e-46 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 630
AccB1I GGYRCC 1 cut(s) 741
AccB7I CCANNNNNTGG 1 cut(s) 417
AccI GTMKAC 1 cut(s) 644
AccIII TCCGGA 1 cut(s) 198
AciI CCGC 6 cut(s) 5, 25, 68, 156, 207, 612
AclWI GGATC 2 cut(s) 97, 209
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 844
AfaI GTAC 1 cut(s) 306
AfeI AGCGCT 1 cut(s) 214
AfiI CCNNNNNNNGG 5 cut(s) 112, 238, 417, 511, 566
AgsI TTSAA 2 cut(s) 854, 881
AjnI CCWGG 1 cut(s) 744
AluBI AGCT 3 cut(s) 193, 710, 718
AluI AGCT 3 cut(s) 193, 710, 718
Alw21I GWGCWC 2 cut(s) 712, 778
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 2 cut(s) 97, 209
Ama87I CYCGRG 1 cut(s) 630
Aor13HI TCCGGA 1 cut(s) 198
Aor51HI AGCGCT 1 cut(s) 214
AoxI GGCC 3 cut(s) 169, 294, 568
ApaI GGGCCC 1 cut(s) 572
ApeKI GCWGC 3 cut(s) 210, 665, 809
ApoI RAATTY 1 cut(s) 844
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 5 cut(s) 22, 295, 568, 569, 595
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 139, 704
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 2 cut(s) 22, 595
BaeGI GKGCMC 1 cut(s) 572
BanI GGYRCC 1 cut(s) 741
BanII GRGCYC 3 cut(s) 538, 572, 712
Bbv12I GWGCWC 2 cut(s) 712, 778
BbvI GCAGC 3 cut(s) 222, 677, 796
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 1 cut(s) 746
BciVI GTATCC 2 cut(s) 254, 508
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 2 cut(s) 290, 705
BfoI RGCGCY 1 cut(s) 216
BfuI GTATCC 2 cut(s) 254, 508
BglII AGATCT 1 cut(s) 477
BisI GCNGC 6 cut(s) 5, 157, 208, 211, 666, 810
BlsI GCNGC 6 cut(s) 6, 158, 209, 212, 667, 811
Bme1390I CCNGG 2 cut(s) 599, 746
Bme18I GGWCC 2 cut(s) 22, 595
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 5 cut(s) 22, 295, 568, 569, 595
BmiI GGNNCC 4 cut(s) 558, 570, 597, 743
BmrFI CCNGG 2 cut(s) 599, 746
BmsI GCATC 2 cut(s) 33, 599
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 348, 839
BpuMI CCSGG 1 cut(s) 599
Bsa29I ATCGAT 2 cut(s) 607, 793
BsaJI CCNNGG 3 cut(s) 166, 270, 519
BsaWI WCCGGW 2 cut(s) 142, 198
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 238, 417, 511, 566
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 172
BseAI TCCGGA 1 cut(s) 198
BseBI CCWGG 1 cut(s) 746
BseCI ATCGAT 2 cut(s) 607, 793
BseDI CCNNGG 3 cut(s) 166, 270, 519
BseGI GGATG 2 cut(s) 162, 607
BseLI CCNNNNNNNGG 5 cut(s) 112, 238, 417, 511, 566
BseMII CTCAG 2 cut(s) 346, 683
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 2 cut(s) 128, 618
BseSI GKGCMC 1 cut(s) 572
BseXI GCAGC 3 cut(s) 222, 677, 796
BseYI CCCAGC 1 cut(s) 862
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 3 cut(s) 171, 296, 570
BshNI GGYRCC 1 cut(s) 741
BshVI ATCGAT 2 cut(s) 607, 793
BsiHKAI GWGCWC 2 cut(s) 712, 778
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 4 cut(s) 131, 143, 199, 599
BslI CCNNNNNNNGG 5 cut(s) 112, 238, 417, 511, 566
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 3 cut(s) 171, 296, 570
BsoBI CYCGRG 1 cut(s) 630
Bsp120I GGGCCC 1 cut(s) 568
Bsp1286I GDGCHC 4 cut(s) 538, 572, 712, 778
Bsp13I TCCGGA 1 cut(s) 198
Bsp143I GATC 9 cut(s) 16, 30, 102, 201, 253, 477, 541, 580, 604
BspACI CCGC 6 cut(s) 5, 25, 68, 156, 207, 612
BspANI GGCC 3 cut(s) 171, 296, 570
BspCNI CTCAG 2 cut(s) 345, 684
BspDI ATCGAT 2 cut(s) 607, 793
BspEI TCCGGA 1 cut(s) 198
BspHI TCATGA 2 cut(s) 343, 562
BspLI GGNNCC 4 cut(s) 558, 570, 597, 743
BspPI GGATC 2 cut(s) 97, 209
BspT107I GGYRCC 1 cut(s) 741
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 166, 270, 519
BssMI GATC 9 cut(s) 16, 30, 102, 201, 253, 477, 541, 580, 604
BssNAI GTATAC 1 cut(s) 645
BssT1I CCWWGG 1 cut(s) 519
Bst1107I GTATAC 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 4 cut(s) 151, 280, 672, 766
Bst6I CTCTTC 1 cut(s) 239
BstC8I GCNNGC 4 cut(s) 53, 89, 93, 488
BstDEI CTNAG 2 cut(s) 332, 692
BstDSI CCRYGG 2 cut(s) 166, 270
BstEII GGTNACC 1 cut(s) 145
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 2 cut(s) 162, 607
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 9 cut(s) 19, 33, 105, 204, 256, 480, 544, 583, 607
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 9 cut(s) 16, 30, 102, 201, 253, 477, 541, 580, 604
BstMWI GCNNNNNNNGC 3 cut(s) 52, 88, 576
BstNI CCWGG 1 cut(s) 746
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 597, 744
BstSLI GKGCMC 1 cut(s) 572
BstV1I GCAGC 3 cut(s) 222, 677, 796
BstX2I RGATCY 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 1 cut(s) 477
BstZ17I GTATAC 1 cut(s) 645
Bsu15I ATCGAT 2 cut(s) 607, 793
BsuI GTATCC 2 cut(s) 254, 508
BsuRI GGCC 3 cut(s) 171, 296, 570
BsuTUI ATCGAT 2 cut(s) 607, 793
BtgI CCRYGG 2 cut(s) 166, 270
BtsCI GGATG 2 cut(s) 162, 607
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 3 cut(s) 53, 429, 452
Cac8I GCNNGC 4 cut(s) 53, 89, 93, 488
CciI TCATGA 2 cut(s) 343, 562
CfoI GCGC 1 cut(s) 215
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 5 cut(s) 22, 295, 568, 569, 595
ClaI ATCGAT 2 cut(s) 607, 793
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 305
CviAII CATG 5 cut(s) 238, 344, 491, 563, 835
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 2 cut(s) 332, 692
DpnI GATC 9 cut(s) 18, 32, 104, 203, 255, 479, 543, 582, 606
DpnII GATC 9 cut(s) 16, 30, 102, 201, 253, 477, 541, 580, 604
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 710
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 3 cut(s) 538, 572, 712
Eco47I GGWCC 2 cut(s) 22, 595
Eco47III AGCGCT 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 710
Eco88I CYCGRG 1 cut(s) 630
Eco91I GGTNACC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 710
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 568
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 744
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 3 cut(s) 538, 572, 712
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 5 cut(s) 241, 347, 494, 566, 838
FatI CATG 5 cut(s) 237, 343, 490, 562, 834
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 6 cut(s) 5, 157, 208, 211, 666, 810
FokI GGATG 2 cut(s) 149, 614
FriOI GRGCYC 3 cut(s) 538, 572, 712
Fsp4HI GCNGC 6 cut(s) 5, 157, 208, 211, 666, 810
FspBI CTAG 2 cut(s) 290, 705
GlaI GCGC 1 cut(s) 214
GluI GCNGC 6 cut(s) 5, 157, 208, 211, 666, 810
GsaI CCCAGC 1 cut(s) 866
GsuI CTGGAG 1 cut(s) 888
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 3 cut(s) 171, 296, 570
HapII CCGG 4 cut(s) 131, 143, 199, 599
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 215
Hin1II CATG 5 cut(s) 241, 347, 494, 566, 838
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HinfI GANTC 3 cut(s) 349, 472, 694
HpaII CCGG 4 cut(s) 131, 143, 199, 599
HphI GGTGA 2 cut(s) 139, 704
Hpy166II GTNNAC 2 cut(s) 497, 645
Hpy188I TCNGA 4 cut(s) 60, 335, 477, 595
Hpy8I GTNNAC 2 cut(s) 497, 645
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 2 cut(s) 722, 860
HpyCH4III ACNGT 4 cut(s) 151, 280, 672, 766
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 5 cut(s) 46, 51, 490, 638, 812
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 88, 576
HpyF3I CTNAG 2 cut(s) 332, 692
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 5 cut(s) 241, 347, 494, 566, 838
HspAI GCGC 1 cut(s) 213
Kpn2I TCCGGA 1 cut(s) 198
Kzo9I GATC 9 cut(s) 16, 30, 102, 201, 253, 477, 541, 580, 604
LmnI GCTCC 3 cut(s) 220, 781, 838
Lsp1109I GCAGC 3 cut(s) 222, 677, 796
LweI GCATC 2 cut(s) 33, 599
MaeI CTAG 2 cut(s) 290, 705
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 4 cut(s) 145, 280, 308, 760
MalI GATC 9 cut(s) 18, 32, 104, 203, 255, 479, 543, 582, 606
MboI GATC 9 cut(s) 16, 30, 102, 201, 253, 477, 541, 580, 604
MboII GAAGA 3 cut(s) 226, 472, 909
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 4 cut(s) 538, 572, 712, 778
MluCI AATT 8 cut(s) 321, 327, 357, 465, 736, 781, 844, 854
MlyI GAGTC 3 cut(s) 358, 481, 703
MmeI TCCRAC 1 cut(s) 573
MroI TCCGGA 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 2 cut(s) 464, 675
MslI CAYNNNNRTG 1 cut(s) 684
MspI CCGG 4 cut(s) 131, 143, 199, 599
MspR9I CCNGG 2 cut(s) 599, 746
MunI CAATTG 1 cut(s) 736
MvaI CCWGG 1 cut(s) 746
MwoI GCNNNNNNNGC 3 cut(s) 52, 88, 576
NciI CCSGG 1 cut(s) 599
NdeII GATC 9 cut(s) 16, 30, 102, 201, 253, 477, 541, 580, 604
NlaIII CATG 5 cut(s) 241, 347, 494, 566, 838
NlaIV GGNNCC 4 cut(s) 558, 570, 597, 743
NmuCI GTSAC 2 cut(s) 145, 280
PaeR7I CTCGAG 1 cut(s) 630
PagI TCATGA 2 cut(s) 343, 562
PdmI GAANNNNTTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 6 cut(s) 6, 158, 209, 212, 667, 811
PleI GAGTC 3 cut(s) 357, 480, 702
PpsI GAGTC 3 cut(s) 357, 480, 702
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 744
PspEI GGTNACC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 862
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 4 cut(s) 558, 570, 597, 743
PspOMI GGGCCC 1 cut(s) 568
PspPI GGNCC 5 cut(s) 22, 295, 568, 569, 595
PspXI VCTCGAGB 1 cut(s) 630
PsuI RGATCY 1 cut(s) 477
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 684
SacI GAGCTC 1 cut(s) 712
SaqAI TTAA 2 cut(s) 464, 675
SatI GCNGC 6 cut(s) 5, 157, 208, 211, 666, 810
Sau3AI GATC 9 cut(s) 16, 30, 102, 201, 253, 477, 541, 580, 604
Sau96I GGNCC 5 cut(s) 22, 295, 568, 569, 595
SchI GAGTC 3 cut(s) 358, 481, 703
ScrFI CCNGG 2 cut(s) 599, 746
SduI GDGCHC 4 cut(s) 538, 572, 712, 778
SfaNI GCATC 2 cut(s) 33, 599
Sfr274I CTCGAG 1 cut(s) 630
SinI GGWCC 2 cut(s) 22, 595
SlaI CTCGAG 1 cut(s) 630
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 3 cut(s) 363, 630, 818
SmoI CTYRAG 3 cut(s) 363, 630, 818
Sse9I AATT 8 cut(s) 321, 327, 357, 465, 736, 781, 844, 854
SsiI CCGC 6 cut(s) 5, 25, 68, 156, 207, 612
SspI AATATT 1 cut(s) 529
SspMI CTAG 2 cut(s) 290, 705
SstI GAGCTC 1 cut(s) 712
StyD4I CCNGG 2 cut(s) 597, 744
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 4 cut(s) 151, 280, 672, 766
TaiI ACGT 1 cut(s) 310
TaqI TCGA 6 cut(s) 33, 111, 256, 607, 631, 793
TasI AATT 8 cut(s) 321, 327, 357, 465, 736, 781, 844, 854
TauI GCSGC 3 cut(s) 7, 159, 210
Tru1I TTAA 2 cut(s) 464, 675
Tru9I TTAA 2 cut(s) 464, 675
TscAI CASTG 3 cut(s) 53, 436, 459
TseFI GTSAC 2 cut(s) 145, 280
TseI GCWGC 3 cut(s) 210, 665, 809
Tsp45I GTSAC 2 cut(s) 145, 280
TspDTI ATGAA 1 cut(s) 226
TspGWI ACGGA 2 cut(s) 77, 259
TspRI CASTG 3 cut(s) 53, 436, 459
Van91I CCANNNNNTGG 1 cut(s) 417
VpaK11BI GGWCC 2 cut(s) 22, 595
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 844
XbaI TCTAGA 1 cut(s) 704
XhoI CTCGAG 1 cut(s) 630
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 2 cut(s) 290, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.