RLG00000005778

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003085
Physical Location & Seq
Forward (+)
1669086 .. 1671696
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005778

Sequence Viewer

Length: 879 bp
ATGGAGTGCAAGACGTTGCCGGAGGCGGAGGTGAAGACGCTGTGCGAGCAAGCGAGGGCGATCCTGGTGGAGGAGTGGAACGTGCAACCGGTCAAGTGCCCGGTTACGGTGTGTGGGGATATACACGGCCAGTTCTACGACCTCATTGAGCTTTTTCGGATAGGAGGCAACGCTCCTGATACTAATTACCTTTTCATGGGTGATTATGTAGATCGTGGATATTATTCTGTGGAGACTGTCACACTTCTGGTGGCTCTGAAAGTTCGTTATAGAGATAGAATTACAATCCTGAGAGGAAATCATGAGAGTCGGCAAATTACTCAAGTGTATGGTTTCTACGACGAGTGTTTAAGAAAATATGGAAATGCCAATGTCTGGAAGTACTTTACTGACTTATTTGATTATCTTCCCCTCACAGCCCTTATTGAGAGTCAGATTTTCTGTTTGCATGGGGGCCTTTCACCATCGTTGGACACATTGGACAATATCCGAGCCTTGGACCGTATCCAGGAGGTTCCACATGAAGGACCAATGTGCGATCTCTTGTGGTCTGATCCTGATGACCGCTGTGGTTGGGGAATATCGCCACGTGGTGCTGGTTATACATTTGGACAGGATATAGCTGCTCAGTTTAATCATACCAATGGACTCAGCCTGATTTCAAGAGCTCATCAGCTTGTCATGGAAGGATACAACTGGTGCCAGGAAAAGAACGTCGTGACTGTTTTTAGTGCACCAAACTATTGTTATCGGTGTGGGAATATGGCTGCAATTTTGGAAATTGGGGAGAATATGGACCAGAATTTTCTACAGTTTGACCCAGCTCCTCGTCAAATTGAGCCTGACAACACACGCAAGACTCCAGATTATTTTTTGTGA

Protein Analysis

293

Amino Acids

33.42

Weight (kDa)

4.83

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 34 - 227 9.2e-47 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 699
AccB7I CCANNNNNTGG 1 cut(s) 375
AciI CCGC 2 cut(s) 26, 565
AclWI GGATC 2 cut(s) 55, 548
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 802
AcvI CACGTG 1 cut(s) 590
AdeI CACNNNGTG 1 cut(s) 593
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 7 cut(s) 70, 106, 196, 375, 496, 508, 524
AgeI ACCGGT 1 cut(s) 88
AgsI TTSAA 1 cut(s) 663
AjnI CCWGG 3 cut(s) 63, 507, 702
AluBI AGCT 5 cut(s) 151, 623, 668, 676, 824
AluI AGCT 5 cut(s) 151, 623, 668, 676, 824
Alw21I GWGCWC 2 cut(s) 670, 736
Alw26I GTCTC 1 cut(s) 227
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 2 cut(s) 55, 548
AoxI GGCC 2 cut(s) 127, 454
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 2 cut(s) 623, 767
ApoI RAATTY 1 cut(s) 802
AsiGI ACCGGT 1 cut(s) 88
AspS9I GGNCC 4 cut(s) 454, 499, 527, 796
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 3 cut(s) 43, 212, 453
AvaII GGWCC 3 cut(s) 499, 527, 796
BaeGI GKGCMC 2 cut(s) 101, 736
BanI GGYRCC 1 cut(s) 699
BanII GRGCYC 1 cut(s) 670
BbrPI CACGTG 1 cut(s) 590
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 2 cut(s) 670, 736
BbvI GCAGC 2 cut(s) 610, 754
BccI CCATC 1 cut(s) 472
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 3 cut(s) 65, 509, 704
BciVI GTATCC 2 cut(s) 515, 683
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 227
BfmI CTRYAG 1 cut(s) 809
BfuI GTATCC 2 cut(s) 515, 683
BisI GCNGC 2 cut(s) 624, 768
BlsI GCNGC 2 cut(s) 625, 769
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 4 cut(s) 65, 101, 509, 704
Bme18I GGWCC 3 cut(s) 499, 527, 796
BmgT120I GGNCC 4 cut(s) 454, 499, 527, 796
BmiI GGNNCC 3 cut(s) 455, 516, 701
BmrFI CCNGG 4 cut(s) 65, 101, 509, 704
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 846
BpuEI CTTGAG 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 495
BsaWI WCCGGW 1 cut(s) 88
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 106, 196, 375, 496, 508, 524
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 2 cut(s) 130, 701
BseBI CCWGG 3 cut(s) 65, 509, 704
BseDI CCNNGG 1 cut(s) 495
BseLI CCNNNNNNNGG 7 cut(s) 70, 106, 196, 375, 496, 508, 524
BseMII CTCAG 3 cut(s) 281, 641, 664
BseNI ACTGG 2 cut(s) 130, 701
BseRI GAGGAG 2 cut(s) 86, 816
BseSI GKGCMC 2 cut(s) 101, 736
BseXI GCAGC 2 cut(s) 610, 754
BseYI CCCAGC 1 cut(s) 820
BshFI GGCC 2 cut(s) 129, 456
BshNI GGYRCC 1 cut(s) 699
BshTI ACCGGT 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 670, 736
BsiSI CCGG 3 cut(s) 20, 89, 101
BslI CCNNNNNNNGG 7 cut(s) 70, 106, 196, 375, 496, 508, 524
BsmAI GTCTC 1 cut(s) 227
BsnI GGCC 2 cut(s) 129, 456
Bsp1286I GDGCHC 3 cut(s) 101, 670, 736
Bsp143I GATC 4 cut(s) 60, 211, 538, 553
BspACI CCGC 2 cut(s) 26, 565
BspANI GGCC 2 cut(s) 129, 456
BspCNI CTCAG 3 cut(s) 282, 640, 663
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 3 cut(s) 455, 516, 701
BspPI GGATC 2 cut(s) 55, 548
BspT107I GGYRCC 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 2 cut(s) 130, 701
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 4 cut(s) 60, 211, 538, 553
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 3 cut(s) 65, 509, 704
Bst4CI ACNGT 5 cut(s) 109, 238, 503, 724, 813
BstBAI YACGTR 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 47, 51
BstDEI CTNAG 3 cut(s) 290, 627, 650
BstENI CCTNNNNNAGG 1 cut(s) 68
BstKTI GATC 4 cut(s) 63, 214, 541, 556
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 60, 211, 538, 553
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 3 cut(s) 65, 509, 704
BstSCI CCNGG 4 cut(s) 63, 99, 507, 702
BstSFI CTRYAG 1 cut(s) 809
BstSLI GKGCMC 2 cut(s) 101, 736
BstV1I GCAGC 2 cut(s) 610, 754
BstV2I GAAGAC 1 cut(s) 41
BsuI GTATCC 2 cut(s) 515, 683
BsuRI GGCC 2 cut(s) 129, 456
Cac8I GCNNGC 2 cut(s) 47, 51
CciI TCATGA 1 cut(s) 301
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 4 cut(s) 454, 499, 527, 796
CseI GACGC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 382
CspAI ACCGGT 1 cut(s) 88
CviAII CATG 5 cut(s) 196, 302, 449, 521, 682
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 3 cut(s) 290, 627, 650
DpnI GATC 4 cut(s) 62, 213, 540, 555
DpnII GATC 4 cut(s) 60, 211, 538, 553
DraIII CACNNNGTG 1 cut(s) 593
EaeI YGGCCR 1 cut(s) 127
EciI GGCGGA 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 668
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 670
Eco47I GGWCC 3 cut(s) 499, 527, 796
Eco53kI GAGCTC 1 cut(s) 668
Eco72I CACGTG 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 668
EcoNI CCTNNNNNAGG 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 454
EcoRII CCWGG 3 cut(s) 63, 507, 702
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 5 cut(s) 199, 305, 452, 524, 685
FatI CATG 5 cut(s) 195, 301, 448, 520, 681
Fnu4HI GCNGC 2 cut(s) 624, 768
FriOI GRGCYC 1 cut(s) 670
Fsp4HI GCNGC 2 cut(s) 624, 768
GluI GCNGC 2 cut(s) 624, 768
GsaI CCCAGC 1 cut(s) 824
GsuI CTGGAG 1 cut(s) 846
HaeIII GGCC 2 cut(s) 129, 456
HapII CCGG 3 cut(s) 20, 89, 101
HgaI GACGC 1 cut(s) 46
Hin1II CATG 5 cut(s) 199, 305, 452, 524, 685
HinfI GANTC 4 cut(s) 307, 430, 648, 859
HpaII CCGG 3 cut(s) 20, 89, 101
HphI GGTGA 3 cut(s) 43, 212, 453
Hpy166II GTNNAC 1 cut(s) 734
Hpy188I TCNGA 5 cut(s) 159, 258, 435, 491, 553
Hpy188III TCNNGA 8 cut(s) 176, 289, 302, 376, 557, 663, 718, 863
Hpy8I GTNNAC 1 cut(s) 734
Hpy99I CGWCG 2 cut(s) 344, 719
HpyAV CCTTC 2 cut(s) 518, 680
HpyCH4III ACNGT 5 cut(s) 109, 238, 503, 724, 813
HpyCH4IV ACGT 4 cut(s) 14, 81, 589, 714
HpyCH4V TGCA 5 cut(s) 9, 85, 448, 734, 770
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 3 cut(s) 290, 627, 650
HpySE526I ACGT 4 cut(s) 14, 81, 589, 714
Hsp92II CATG 5 cut(s) 199, 305, 452, 524, 685
Kzo9I GATC 4 cut(s) 60, 211, 538, 553
LmnI GCTCC 2 cut(s) 178, 829
Lsp1109I GCAGC 2 cut(s) 610, 754
MaeII ACGT 4 cut(s) 14, 81, 589, 714
MaeIII GTNAC 3 cut(s) 103, 238, 718
MalI GATC 4 cut(s) 62, 213, 540, 555
MboI GATC 4 cut(s) 60, 211, 538, 553
MboII GAAGA 2 cut(s) 46, 398
MhlI GDGCHC 3 cut(s) 101, 670, 736
MluCI AATT 7 cut(s) 184, 279, 315, 771, 780, 802, 834
MlyI GAGTC 4 cut(s) 316, 439, 642, 853
MmeI TCCRAC 1 cut(s) 450
MseI TTAA 2 cut(s) 350, 633
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 567
MspI CCGG 3 cut(s) 20, 89, 101
MspR9I CCNGG 4 cut(s) 65, 101, 509, 704
MvaI CCWGG 3 cut(s) 65, 509, 704
MwoI GCNNNNNNNGC 1 cut(s) 46
NciI CCSGG 1 cut(s) 101
NdeII GATC 4 cut(s) 60, 211, 538, 553
NlaIII CATG 5 cut(s) 199, 305, 452, 524, 685
NlaIV GGNNCC 3 cut(s) 455, 516, 701
NmuCI GTSAC 2 cut(s) 238, 718
PagI TCATGA 1 cut(s) 301
PflMI CCANNNNNTGG 1 cut(s) 375
PfoI TCCNGGA 1 cut(s) 507
PinAI ACCGGT 1 cut(s) 88
PkrI GCNGC 2 cut(s) 625, 769
PleI GAGTC 4 cut(s) 315, 438, 642, 853
PmaCI CACGTG 1 cut(s) 590
PmlI CACGTG 1 cut(s) 590
PpsI GAGTC 4 cut(s) 315, 438, 642, 853
Ppu21I YACGTR 1 cut(s) 590
Psp124BI GAGCTC 1 cut(s) 670
Psp6I CCWGG 3 cut(s) 63, 507, 702
PspCI CACGTG 1 cut(s) 590
PspFI CCCAGC 1 cut(s) 820
PspGI CCWGG 3 cut(s) 63, 507, 702
PspN4I GGNNCC 3 cut(s) 455, 516, 701
PspPI GGNCC 4 cut(s) 454, 499, 527, 796
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 1 cut(s) 670
SaqAI TTAA 2 cut(s) 350, 633
SatI GCNGC 2 cut(s) 624, 768
Sau3AI GATC 4 cut(s) 60, 211, 538, 553
Sau96I GGNCC 4 cut(s) 454, 499, 527, 796
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 4 cut(s) 316, 439, 642, 853
ScrFI CCNGG 4 cut(s) 65, 101, 509, 704
SduI GDGCHC 3 cut(s) 101, 670, 736
SfcI CTRYAG 1 cut(s) 809
SinI GGWCC 3 cut(s) 499, 527, 796
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 7 cut(s) 184, 279, 315, 771, 780, 802, 834
SsiI CCGC 2 cut(s) 26, 565
SstI GAGCTC 1 cut(s) 670
StyD4I CCNGG 4 cut(s) 63, 99, 507, 702
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 5 cut(s) 109, 238, 503, 724, 813
TaiI ACGT 4 cut(s) 17, 84, 592, 717
TasI AATT 7 cut(s) 184, 279, 315, 771, 780, 802, 834
TatI WGTACW 1 cut(s) 381
Tru1I TTAA 2 cut(s) 350, 633
Tru9I TTAA 2 cut(s) 350, 633
TseFI GTSAC 2 cut(s) 238, 718
TseI GCWGC 2 cut(s) 623, 767
Tsp45I GTSAC 2 cut(s) 238, 718
TspDTI ATGAA 2 cut(s) 184, 537
Van91I CCANNNNNTGG 1 cut(s) 375
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 3 cut(s) 499, 527, 796
XagI CCTNNNNNAGG 1 cut(s) 68
XapI RAATTY 1 cut(s) 802
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.