FvH4_5g00460

lipid transfer-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013181
Physical Location & Seq
Forward (+)
297418 .. 299279
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00460.t1

Sequence Viewer

Length: 456 bp
ATGGGTTGCGGCAACATTTCGACACTGACGGTCATGGTGGTGGTGGTGGCCTTGTTGAGCTGTATGGCTCCCCCAAGCAAGGCACAGCAGCAGACCTGCGCTAATGACCTGCTGCCTTGTGGAGACTACCTCAACAACACCGACCAACCATCCACCACCTGCTGCACCGCCATCAAACAGACTGTGGATACTCAGCTCACCTGCCTCTGCACTCTATATACCACCCCCGGCCTTCTCAAGGCCCTTGGCGCCAACATCACCTCCGCTGTTAGAATCGCCAACGCTTGCGGCGTCAACTCCCTCGATGCCGATAAGTGCAACGCTGTCCGGGGTCAAGCGCCTGTTTCTCCACCTACAGCTGTACCGGGAGGTGATGGTAGCAGTGGTTCCGTCAGGATTGTGTGGACCGGATTCCCTGCTCTGCTCTTATTCTGGGCTTCCGTCATGTATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

152

Amino Acids

15.62

Weight (kDa)

5.95

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 15 - 106 6.4e-14 Probable lipid transfer
Tryp_alpha_amyl PF00234 35 - 100 1e-05 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013181)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62790 AT1G62790
fragaria_vesca FvH4_5g00460
malus_domestica MD04G1081000.v1.1 MD06G1064500.v1.1
prunus_persica Prupe.5G077100_v2.0.a1
pyrus_communis pycom06g05560
rosa_chinensis RchiOBHm_Chr7g0202051
rosa_laevigata RLG00000003629
rosa_multiflora Rmu_co8366653.1_g000001 Rmu_sc0006592.1_g000005
rosa_roxburghii Rroxscaffold_3G00254900
rosa_rugosa Rorug07G0067700 Rorug07G0067700
rosa_samantha Rh7AG195000 Rh7BG196400 Rh7DG201600
rosa_wichuraiana Rw7G017080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 167, 209
Acc36I ACCTGC 4 cut(s) 104, 117, 167, 209
AccB1I GGYRCC 1 cut(s) 248
AciI CCGC 4 cut(s) 9, 168, 264, 288
AcyI GRCGYC 2 cut(s) 249, 291
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 2 cut(s) 79, 238
AluBI AGCT 3 cut(s) 60, 196, 359
AluI AGCT 3 cut(s) 60, 196, 359
Alw26I GTCTC 1 cut(s) 117
AoxI GGCC 3 cut(s) 48, 229, 240
ApeKI GCWGC 3 cut(s) 88, 112, 162
AspLEI GCGC 3 cut(s) 101, 251, 340
AspS9I GGNCC 2 cut(s) 241, 405
AsuC2I CCSGG 3 cut(s) 228, 329, 366
AsuHPI GGTGA 3 cut(s) 190, 250, 383
AvaII GGWCC 1 cut(s) 405
BaeI ACNNNNGTAYC 2 cut(s) 345, 378
BanI GGYRCC 1 cut(s) 248
BbvI GCAGC 3 cut(s) 99, 100, 149
BccI CCATC 3 cut(s) 157, 179, 368
BciVI GTATCC 1 cut(s) 181
BcnI CCSGG 3 cut(s) 228, 329, 366
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 1 cut(s) 454
BfmI CTRYAG 1 cut(s) 354
BfoI RGCGCY 2 cut(s) 252, 341
BfuAI ACCTGC 4 cut(s) 104, 117, 167, 209
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 5 cut(s) 10, 89, 113, 163, 289
BlsI GCNGC 5 cut(s) 11, 90, 114, 164, 290
Bme1390I CCNGG 3 cut(s) 228, 329, 366
Bme18I GGWCC 1 cut(s) 405
BmgT120I GGNCC 2 cut(s) 241, 405
BmiI GGNNCC 3 cut(s) 69, 250, 388
BmrFI CCNGG 3 cut(s) 228, 329, 366
BmsI GCATC 1 cut(s) 295
BplI GAGNNNNNCTC 2 cut(s) 114, 146
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 3 cut(s) 228, 329, 366
BsaHI GRCGYC 2 cut(s) 249, 291
BsaJI CCNNGG 3 cut(s) 226, 244, 328
BsaWI WCCGGW 1 cut(s) 407
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 238
BseDI CCNNGG 3 cut(s) 226, 244, 328
BseGI GGATG 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 79, 238
BseMII CTCAG 1 cut(s) 206
BseXI GCAGC 3 cut(s) 99, 100, 149
BsgI GTGCAG 2 cut(s) 148, 193
BshFI GGCC 3 cut(s) 50, 231, 242
BshNI GGYRCC 1 cut(s) 248
BsiSI CCGG 4 cut(s) 228, 328, 365, 408
BslI CCNNNNNNNGG 2 cut(s) 79, 238
BsmAI GTCTC 1 cut(s) 117
BsnI GGCC 3 cut(s) 50, 231, 242
BspACI CCGC 4 cut(s) 9, 168, 264, 288
BspANI GGCC 3 cut(s) 50, 231, 242
BspCNI CTCAG 1 cut(s) 205
BspLI GGNNCC 3 cut(s) 69, 250, 388
BspMI ACCTGC 4 cut(s) 104, 117, 167, 209
BspT107I GGYRCC 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 226, 244, 328
BssNI GRCGYC 2 cut(s) 249, 291
BssT1I CCWWGG 1 cut(s) 244
Bst4CI ACNGT 2 cut(s) 31, 184
BstACI GRCGYC 2 cut(s) 249, 291
BstC8I GCNNGC 1 cut(s) 286
BstDEI CTNAG 1 cut(s) 192
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 1 cut(s) 149
BstH2I RGCGCY 2 cut(s) 252, 341
BstHHI GCGC 3 cut(s) 101, 251, 340
BstMAI GTCTC 1 cut(s) 117
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstSCI CCNGG 3 cut(s) 226, 327, 364
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 3 cut(s) 99, 100, 149
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 3 cut(s) 50, 231, 242
BtsCI GGATG 1 cut(s) 149
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 2 cut(s) 23, 388
BveI ACCTGC 4 cut(s) 104, 117, 167, 209
Cac8I GCNNGC 1 cut(s) 286
CfoI GCGC 3 cut(s) 101, 251, 340
Cfr13I GGNCC 2 cut(s) 241, 405
CseI GACGC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 362
CviAII CATG 2 cut(s) 34, 445
CviJI RGCY 8 cut(s) 50, 60, 68, 196, 231, 242, 359, 437
CviKI_1 RGCY 8 cut(s) 50, 60, 68, 196, 231, 242, 359, 437
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 1 cut(s) 192
DinI GGCGCC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 405
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 244
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 2 cut(s) 37, 448
FaiI YATR 5 cut(s) 35, 65, 217, 219, 446
FatI CATG 2 cut(s) 33, 444
Fnu4HI GCNGC 5 cut(s) 10, 89, 113, 163, 289
FokI GGATG 1 cut(s) 136
Fsp4HI GCNGC 5 cut(s) 10, 89, 113, 163, 289
FspBI CTAG 1 cut(s) 454
GlaI GCGC 3 cut(s) 100, 250, 339
GluI GCNGC 5 cut(s) 10, 89, 113, 163, 289
HaeII RGCGCY 2 cut(s) 252, 341
HaeIII GGCC 3 cut(s) 50, 231, 242
HapII CCGG 4 cut(s) 228, 328, 365, 408
HgaI GACGC 1 cut(s) 280
HhaI GCGC 3 cut(s) 101, 251, 340
Hin1I GRCGYC 2 cut(s) 249, 291
Hin1II CATG 2 cut(s) 37, 448
Hin6I GCGC 3 cut(s) 99, 249, 338
HinP1I GCGC 3 cut(s) 99, 249, 338
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 2 cut(s) 273, 411
HpaII CCGG 4 cut(s) 228, 328, 365, 408
HphI GGTGA 3 cut(s) 190, 250, 383
Hpy166II GTNNAC 2 cut(s) 295, 405
Hpy188III TCNNGA 1 cut(s) 394
Hpy8I GTNNAC 2 cut(s) 295, 405
HpyAV CCTTC 1 cut(s) 242
HpyCH4III ACNGT 2 cut(s) 31, 184
HpyCH4V TGCA 3 cut(s) 165, 210, 318
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 1 cut(s) 192
Hsp92I GRCGYC 2 cut(s) 249, 291
Hsp92II CATG 2 cut(s) 37, 448
HspAI GCGC 3 cut(s) 99, 249, 338
KasI GGCGCC 1 cut(s) 248
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 3 cut(s) 99, 100, 149
LweI GCATC 1 cut(s) 295
MaeI CTAG 1 cut(s) 454
Mly113I GGCGCC 1 cut(s) 249
MnlI CCTC 5 cut(s) 140, 215, 271, 311, 362
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 2 cut(s) 266, 359
MspI CCGG 4 cut(s) 228, 328, 365, 408
MspR9I CCNGG 3 cut(s) 228, 329, 366
MwoI GCNNNNNNNGC 1 cut(s) 248
NarI GGCGCC 1 cut(s) 249
NciI CCSGG 3 cut(s) 228, 329, 366
NlaIII CATG 2 cut(s) 37, 448
NlaIV GGNNCC 3 cut(s) 69, 250, 388
PaqCI CACCTGC 2 cut(s) 167, 209
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 2 cut(s) 273, 411
PkrI GCNGC 5 cut(s) 11, 90, 114, 164, 290
PluTI GGCGCC 1 cut(s) 252
PspN4I GGNNCC 3 cut(s) 69, 250, 388
PspPI GGNCC 2 cut(s) 241, 405
PsrI GAACNNNNNNTAC 2 cut(s) 370, 402
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 38
SatI GCNGC 5 cut(s) 10, 89, 113, 163, 289
Sau96I GGNCC 2 cut(s) 241, 405
ScrFI CCNGG 3 cut(s) 228, 329, 366
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 354
SfoI GGCGCC 1 cut(s) 250
SinI GGWCC 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SsiI CCGC 4 cut(s) 9, 168, 264, 288
SspDI GGCGCC 1 cut(s) 248
SspMI CTAG 1 cut(s) 454
StyD4I CCNGG 3 cut(s) 226, 327, 364
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 2 cut(s) 31, 184
TaqI TCGA 2 cut(s) 20, 303
TauI GCSGC 2 cut(s) 12, 291
TfiI GAWTC 2 cut(s) 273, 411
TscAI CASTG 2 cut(s) 30, 388
TseI GCWGC 3 cut(s) 88, 112, 162
TspGWI ACGGA 2 cut(s) 379, 430
TspRI CASTG 2 cut(s) 30, 388
VpaK11BI GGWCC 1 cut(s) 405
XagI CCTNNNNNAGG 1 cut(s) 236
XspI CTAG 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.