MD06G1064500.v1.1

Lipid transfer-like protein VAS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013181
Physical Location & Seq
Reverse (-)
13258992 .. 13264086
5095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1064500.v1.1.491

Sequence Viewer

Length: 465 bp
ATGGGCAGCTATAGCATTTCCAAGTTGGCGATGGCCACGGTGGTTGTGTTGTTAGGCATGGCAAGTTTGGTCGGACGGTCAGCCGGTCAAACGACGCCTGCTTGTGCTCAGGACCTGATACCCTGCGCCGACTACCTCAAGAACACAGCTCAGCCAGCTGCCAACTGCTGCACCGCCATCAAGCAGGCGGTGGCCAACCAGTTTGCGTGTCTCTGCAACCTCTTTTTCACTCCCGGACTCCTTCAGAGTTTTGGCGGCAACGCCACTTCCGGTCTCAGAATCGCCACCGCTTGCGGCGAATCAATGGATACCGCCAAATGCAAAGCTGCCATAGGTGCTCCTACACCAGAATCCACACCTGGAACCCCAGGAACGCCGGGAAATGACGGTAGCGGTTCCGGCAAGGCTGAGTGGACTGCATTTTCTGCTCCGCTCTTATTATGGGCTTCTGTCATGTTATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

155

Amino Acids

15.55

Weight (kDa)

7.47

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 26 - 107 3e-13 Probable lipid transfer
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013181)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62790 AT1G62790
fragaria_vesca FvH4_5g00460
malus_domestica MD04G1081000.v1.1 MD06G1064500.v1.1
prunus_persica Prupe.5G077100_v2.0.a1
pyrus_communis pycom06g05560
rosa_chinensis RchiOBHm_Chr7g0202051
rosa_laevigata RLG00000003629
rosa_multiflora Rmu_co8366653.1_g000001 Rmu_sc0006592.1_g000005
rosa_roxburghii Rroxscaffold_3G00254900
rosa_rugosa Rorug07G0067700 Rorug07G0067700
rosa_samantha Rh7AG195000 Rh7BG196400 Rh7DG201600
rosa_wichuraiana Rw7G017080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 433
AciI CCGC 8 cut(s) 174, 188, 255, 288, 294, 312, 393, 431
AcoI YGGCCR 2 cut(s) 33, 192
AcuI CTGAAG 1 cut(s) 227
AcyI GRCGYC 1 cut(s) 95
AjnI CCWGG 2 cut(s) 358, 367
AluBI AGCT 4 cut(s) 9, 149, 158, 326
AluI AGCT 4 cut(s) 9, 149, 158, 326
Alw21I GWGCWC 2 cut(s) 109, 340
Alw26I GTCTC 2 cut(s) 215, 278
AlwNI CAGNNNCTG 1 cut(s) 115
AoxI GGCC 2 cut(s) 33, 192
ApeKI GCWGC 4 cut(s) 6, 158, 168, 326
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 2 cut(s) 234, 378
AvaII GGWCC 1 cut(s) 112
BalI TGGCCA 2 cut(s) 35, 194
Bbv12I GWGCWC 2 cut(s) 109, 340
BbvI GCAGC 4 cut(s) 18, 145, 155, 313
BccI CCATC 2 cut(s) 25, 185
BciT130I CCWGG 2 cut(s) 360, 369
BciVI GTATCC 1 cut(s) 301
BcnI CCSGG 2 cut(s) 234, 378
BcoDI GTCTC 2 cut(s) 215, 278
BfmI CTRYAG 1 cut(s) 10
BfuI GTATCC 1 cut(s) 301
BisI GCNGC 6 cut(s) 7, 159, 169, 256, 295, 327
BlpI GCTNAGC 1 cut(s) 150
BlsI GCNGC 6 cut(s) 8, 160, 170, 257, 296, 328
Bme1390I CCNGG 4 cut(s) 234, 360, 369, 378
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 2 cut(s) 364, 397
BmrFI CCNGG 4 cut(s) 234, 360, 369, 378
Bpu10I CCTNAGC 1 cut(s) 108
Bpu1102I GCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 2 cut(s) 234, 378
BsaHI GRCGYC 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 2 cut(s) 36, 367
BsaWI WCCGGW 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 199
BseBI CCWGG 2 cut(s) 360, 369
BseDI CCNNGG 2 cut(s) 36, 367
BseMII CTCAG 4 cut(s) 122, 164, 289, 399
BseNI ACTGG 1 cut(s) 199
BseXI GCAGC 4 cut(s) 18, 145, 155, 313
BsgI GTGCAG 1 cut(s) 154
BshFI GGCC 2 cut(s) 35, 194
BsiHKAI GWGCWC 2 cut(s) 109, 340
BsiSI CCGG 5 cut(s) 84, 234, 270, 377, 399
BsmAI GTCTC 2 cut(s) 215, 278
BsnI GGCC 2 cut(s) 35, 194
Bso31I GGTCTC 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 109, 340
Bsp1720I GCTNAGC 1 cut(s) 150
BspACI CCGC 8 cut(s) 174, 188, 255, 288, 294, 312, 393, 431
BspANI GGCC 2 cut(s) 35, 194
BspCNI CTCAG 4 cut(s) 121, 163, 288, 400
BspLI GGNNCC 2 cut(s) 364, 397
BspTNI GGTCTC 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 36, 367
BssNI GRCGYC 1 cut(s) 95
Bst2UI CCWGG 2 cut(s) 360, 369
Bst4CI ACNGT 3 cut(s) 40, 78, 389
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 4 cut(s) 99, 156, 186, 292
BstDEI CTNAG 4 cut(s) 108, 150, 275, 408
BstDSI CCRYGG 1 cut(s) 36
BstHHI GCGC 1 cut(s) 128
BstMAI GTCTC 2 cut(s) 215, 278
BstMWI GCNNNNNNNGC 5 cut(s) 12, 155, 335, 399, 425
BstNI CCWGG 2 cut(s) 360, 369
BstSCI CCNGG 4 cut(s) 232, 358, 367, 376
BstSFI CTRYAG 1 cut(s) 10
BstV1I GCAGC 4 cut(s) 18, 145, 155, 313
BsuI GTATCC 1 cut(s) 301
BsuRI GGCC 2 cut(s) 35, 194
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 44
Cac8I GCNNGC 4 cut(s) 99, 156, 186, 292
CaiI CAGNNNCTG 1 cut(s) 115
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 112
CseI GACGC 1 cut(s) 103
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviAII CATG 2 cut(s) 58, 454
DdeI CTNAG 4 cut(s) 108, 150, 275, 408
EaeI YGGCCR 2 cut(s) 33, 192
Eco31I GGTCTC 1 cut(s) 278
Eco47I GGWCC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 358, 367
FaeI CATG 2 cut(s) 61, 457
FaiI YATR 6 cut(s) 12, 59, 332, 442, 455, 460
FatI CATG 2 cut(s) 57, 453
Fnu4HI GCNGC 6 cut(s) 7, 159, 169, 256, 295, 327
Fsp4HI GCNGC 6 cut(s) 7, 159, 169, 256, 295, 327
GlaI GCGC 1 cut(s) 127
GluI GCNGC 6 cut(s) 7, 159, 169, 256, 295, 327
HaeIII GGCC 2 cut(s) 35, 194
HapII CCGG 5 cut(s) 84, 234, 270, 377, 399
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 2 cut(s) 61, 457
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 4 cut(s) 237, 279, 299, 350
HpaII CCGG 5 cut(s) 84, 234, 270, 377, 399
Hpy166II GTNNAC 1 cut(s) 414
Hpy188I TCNGA 3 cut(s) 74, 246, 278
Hpy188III TCNNGA 2 cut(s) 110, 139
Hpy8I GTNNAC 1 cut(s) 414
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 3 cut(s) 40, 78, 389
HpyCH4V TGCA 4 cut(s) 171, 216, 321, 419
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 155, 335, 399, 425
HpyF3I CTNAG 4 cut(s) 108, 150, 275, 408
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 2 cut(s) 61, 457
HspAI GCGC 1 cut(s) 126
LmnI GCTCC 2 cut(s) 343, 433
Lsp1109I GCAGC 4 cut(s) 18, 145, 155, 313
MbiI CCGCTC 1 cut(s) 433
MhlI GDGCHC 2 cut(s) 109, 340
MlsI TGGCCA 2 cut(s) 35, 194
MluNI TGGCCA 2 cut(s) 35, 194
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 2 cut(s) 146, 230
Mox20I TGGCCA 2 cut(s) 35, 194
MscI TGGCCA 2 cut(s) 35, 194
Msp20I TGGCCA 2 cut(s) 35, 194
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 5 cut(s) 84, 234, 270, 377, 399
MspR9I CCNGG 4 cut(s) 234, 360, 369, 378
MvaI CCWGG 2 cut(s) 360, 369
MwoI GCNNNNNNNGC 5 cut(s) 12, 155, 335, 399, 425
NciI CCSGG 2 cut(s) 234, 378
NlaIII CATG 2 cut(s) 61, 457
NlaIV GGNNCC 2 cut(s) 364, 397
PfeI GAWTC 3 cut(s) 279, 299, 350
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 6 cut(s) 8, 160, 170, 257, 296, 328
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 112
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 358, 367
PspGI CCWGG 2 cut(s) 358, 367
PspN4I GGNNCC 2 cut(s) 364, 397
PspPI GGNCC 1 cut(s) 112
PspPPI RGGWCCY 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 158
SatI GCNGC 6 cut(s) 7, 159, 169, 256, 295, 327
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 4 cut(s) 234, 360, 369, 378
SduI GDGCHC 2 cut(s) 109, 340
SetI ASST 9 cut(s) 11, 117, 138, 151, 160, 222, 328, 337, 361
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SsiI CCGC 8 cut(s) 174, 188, 255, 288, 294, 312, 393, 431
StyD4I CCNGG 4 cut(s) 232, 358, 367, 376
TaaI ACNGT 3 cut(s) 40, 78, 389
TauI GCSGC 2 cut(s) 258, 297
TfiI GAWTC 3 cut(s) 279, 299, 350
TseI GCWGC 4 cut(s) 6, 158, 168, 326
VpaK11BI GGWCC 1 cut(s) 112
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.