FvH4_5g01275

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006923
Physical Location & Seq
Reverse (-)
798058 .. 798785
728 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01275.t1

Sequence Viewer

Length: 480 bp
ATGCCGTCTTCAAATTTCGACTTCCTTTTCCACATCTTCATCTCTGACGTCGCCGGCAATAAAGACTCCACCACCCCAGAATCCAGTCGGAAAAGGAAACTCCGAGACGATCATCAAATGAGCGTCTCGTTTATGTGGAACGTTTTCAGAAAGTGCTACTGCTCCGACGACCGATCAAGGCGGGTCGTCAACCTCGATTTCCTAACGGCCGAGTTTAAAGATCTCAAGTACCAGCTGTTCACCTTCGTCGACGAGGACGACATCGGCGTCATCGATCAGATGATCACCAAAATTGGCCGTCCACTTTACGGTGGCGCCAAGTTTGAGGAGGTTAACGAGATGGTGCACCAGGTACGACAAGCCGTTGTTAAAGCTTTCCGAACCGGACCTCGCCGGGAGAAGCTTGACGTCATGGTCACTGTCCAGAACCAAGTCGGAATGCCGGTTGATGAATATGAAGAAGTTCTCCGAGACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.6

Weight (kDa)

5.65

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 266, 413
AatII GACGTC 2 cut(s) 51, 411
AccB1I GGYRCC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 249
AciI CCGC 1 cut(s) 181
AclI AACGTT 1 cut(s) 141
AcoI YGGCCR 2 cut(s) 207, 295
AcsI RAATTY 1 cut(s) 13
AcyI GRCGYC 4 cut(s) 48, 267, 315, 408
AfaI GTAC 2 cut(s) 230, 354
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 1 cut(s) 12
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 3 cut(s) 235, 374, 403
AluI AGCT 3 cut(s) 235, 374, 403
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 3 cut(s) 99, 130, 465
Alw44I GTGCAC 1 cut(s) 344
AoxI GGCC 2 cut(s) 207, 295
ApaLI GTGCAC 1 cut(s) 344
ApoI RAATTY 1 cut(s) 13
Asp700I GAANNNNTTC 2 cut(s) 143, 462
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 386
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 2 cut(s) 232, 277
AvaII GGWCC 1 cut(s) 386
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 348
BccI CCATC 1 cut(s) 334
BceAI ACGGC 3 cut(s) 222, 282, 347
BciT130I CCWGG 1 cut(s) 350
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 3 cut(s) 99, 130, 465
BfoI RGCGCY 1 cut(s) 318
BglII AGATCT 1 cut(s) 220
Bme1390I CCNGG 2 cut(s) 350, 395
Bme18I GGWCC 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 2 cut(s) 350, 395
BpuEI CTTGAG 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 395
Bsa29I ATCGAT 1 cut(s) 273
BsaHI GRCGYC 4 cut(s) 48, 267, 315, 408
BsaWI WCCGGW 1 cut(s) 383
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse118I RCCGGY 2 cut(s) 53, 442
Bse1I ACTGG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 350
BseCI ATCGAT 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 308
BseNI ACTGG 1 cut(s) 84
BseRI GAGGAG 1 cut(s) 341
BseSI GKGCMC 1 cut(s) 348
BseX3I CGGCCG 1 cut(s) 207
Bsh1285I CGRYCG 2 cut(s) 172, 210
BshFI GGCC 2 cut(s) 209, 297
BshNI GGYRCC 1 cut(s) 314
BshVI ATCGAT 1 cut(s) 273
BsiEI CGRYCG 2 cut(s) 172, 210
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 4 cut(s) 54, 384, 394, 443
BslI CCNNNNNNNGG 1 cut(s) 308
BsmAI GTCTC 3 cut(s) 99, 130, 465
BsmBI CGTCTC 2 cut(s) 99, 130
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 2 cut(s) 209, 297
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 5 cut(s) 109, 173, 220, 274, 282
BspACI CCGC 1 cut(s) 181
BspANI GGCC 2 cut(s) 209, 297
BspDI ATCGAT 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 316
BspT107I GGYRCC 1 cut(s) 314
BsrFI RCCGGY 2 cut(s) 53, 442
BsrI ACTGG 1 cut(s) 84
BssAI RCCGGY 2 cut(s) 53, 442
BssMI GATC 5 cut(s) 109, 173, 220, 274, 282
BssNI GRCGYC 4 cut(s) 48, 267, 315, 408
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 2 cut(s) 311, 421
BstACI GRCGYC 4 cut(s) 48, 267, 315, 408
BstC8I GCNNGC 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 5 cut(s) 112, 176, 223, 277, 285
BstMAI GTCTC 3 cut(s) 99, 130, 465
BstMBI GATC 5 cut(s) 109, 173, 220, 274, 282
BstMCI CGRYCG 2 cut(s) 172, 210
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 2 cut(s) 348, 393
BstSLI GKGCMC 1 cut(s) 348
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BstZI CGGCCG 1 cut(s) 207
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 209, 297
BsuTUI ATCGAT 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 417
Cac8I GCNNGC 1 cut(s) 55
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 2 cut(s) 53, 442
Cfr13I GGNCC 1 cut(s) 386
ClaI ATCGAT 1 cut(s) 273
CseI GACGC 2 cut(s) 112, 256
CsiI ACCWGGT 1 cut(s) 348
Csp6I GTAC 2 cut(s) 229, 353
CviAII CATG 1 cut(s) 412
CviJI RGCY 6 cut(s) 209, 235, 297, 362, 374, 403
CviKI_1 RGCY 6 cut(s) 209, 235, 297, 362, 374, 403
CviQI GTAC 2 cut(s) 229, 353
DinI GGCGCC 1 cut(s) 316
DpnI GATC 5 cut(s) 111, 175, 222, 276, 284
DpnII GATC 5 cut(s) 109, 173, 220, 274, 282
DraI TTTAAA 1 cut(s) 217
DrdI GACNNNNNNGTC 2 cut(s) 266, 413
DseDI GACNNNNNNGTC 2 cut(s) 266, 413
EaeI YGGCCR 2 cut(s) 207, 295
EagI CGGCCG 1 cut(s) 207
EclXI CGGCCG 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 386
Eco52I CGGCCG 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 348
EgeI GGCGCC 1 cut(s) 316
EheI GGCGCC 1 cut(s) 316
Esp3I CGTCTC 2 cut(s) 99, 130
FaeI CATG 1 cut(s) 415
FaiI YATR 3 cut(s) 134, 413, 456
FatI CATG 1 cut(s) 411
FauI CCCGC 1 cut(s) 174
FbaI TGATCA 1 cut(s) 282
FblI GTMKAC 1 cut(s) 249
GlaI GCGC 1 cut(s) 316
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 2 cut(s) 209, 297
HapII CCGG 4 cut(s) 54, 384, 394, 443
HgaI GACGC 2 cut(s) 112, 256
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 4 cut(s) 48, 267, 315, 408
Hin1II CATG 1 cut(s) 415
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 3 cut(s) 190, 250, 334
HindII GTYRAC 3 cut(s) 190, 250, 334
HindIII AAGCTT 2 cut(s) 372, 401
HinfI GANTC 2 cut(s) 65, 80
HpaI GTTAAC 1 cut(s) 334
HpaII CCGG 4 cut(s) 54, 384, 394, 443
HphI GGTGA 2 cut(s) 232, 277
Hpy166II GTNNAC 6 cut(s) 190, 240, 250, 302, 334, 346
Hpy188I TCNGA 9 cut(s) 46, 90, 104, 149, 166, 279, 380, 437, 470
Hpy188III TCNNGA 1 cut(s) 424
Hpy8I GTNNAC 6 cut(s) 190, 240, 250, 302, 334, 346
Hpy99I CGWCG 4 cut(s) 53, 170, 251, 254
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 2 cut(s) 311, 421
HpyCH4IV ACGT 3 cut(s) 48, 141, 408
HpyCH4V TGCA 1 cut(s) 346
HpySE526I ACGT 3 cut(s) 48, 141, 408
Hsp92I GRCGYC 4 cut(s) 48, 267, 315, 408
Hsp92II CATG 1 cut(s) 415
HspAI GCGC 1 cut(s) 315
KasI GGCGCC 1 cut(s) 314
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 282
KspAI GTTAAC 1 cut(s) 334
Kzo9I GATC 5 cut(s) 109, 173, 220, 274, 282
LmnI GCTCC 1 cut(s) 167
MabI ACCWGGT 1 cut(s) 348
MaeII ACGT 3 cut(s) 48, 141, 408
MaeIII GTNAC 1 cut(s) 415
MalI GATC 5 cut(s) 111, 175, 222, 276, 284
MboI GATC 5 cut(s) 109, 173, 220, 274, 282
MboII GAAGA 2 cut(s) 28, 470
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 2 cut(s) 13, 291
Mly113I GGCGCC 1 cut(s) 315
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 3 cut(s) 68, 189, 415
MnlI CCTC 5 cut(s) 203, 247, 319, 322, 399
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 2 cut(s) 143, 462
MseI TTAA 3 cut(s) 216, 333, 369
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 4 cut(s) 54, 384, 394, 443
MspR9I CCNGG 2 cut(s) 350, 395
Mva1269I GAATGC 1 cut(s) 444
MvaI CCWGG 1 cut(s) 350
NaeI GCCGGC 1 cut(s) 55
NarI GGCGCC 1 cut(s) 315
NciI CCSGG 1 cut(s) 395
NdeII GATC 5 cut(s) 109, 173, 220, 274, 282
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 1 cut(s) 415
NlaIV GGNNCC 1 cut(s) 316
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 415
PcsI WCGNNNNNNNCGW 4 cut(s) 192, 255, 264, 270
PctI GAATGC 1 cut(s) 444
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 2 cut(s) 143, 462
PfeI GAWTC 1 cut(s) 80
PleI GAGTC 1 cut(s) 59
PluTI GGCGCC 1 cut(s) 318
PpsI GAGTC 1 cut(s) 59
Psp1406I AACGTT 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 1 cut(s) 386
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
PsuI RGATCY 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 2 cut(s) 230, 354
RsaNI GTAC 2 cut(s) 229, 353
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 3 cut(s) 216, 333, 369
Sau3AI GATC 5 cut(s) 109, 173, 220, 274, 282
Sau96I GGNCC 1 cut(s) 386
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 350, 395
SduI GDGCHC 1 cut(s) 348
SexAI ACCWGGT 1 cut(s) 348
SfoI GGCGCC 1 cut(s) 316
SgrDI CGTCGACG 1 cut(s) 248
SinI GGWCC 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 2 cut(s) 13, 291
SsiI CCGC 1 cut(s) 181
SspDI GGCGCC 1 cut(s) 314
StyD4I CCNGG 2 cut(s) 348, 393
TaaI ACNGT 2 cut(s) 311, 421
TaiI ACGT 3 cut(s) 51, 144, 411
TaqI TCGA 4 cut(s) 18, 195, 249, 273
TaqII GACCGA 1 cut(s) 186
TasI AATT 2 cut(s) 13, 291
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 3 cut(s) 216, 333, 369
Tru9I TTAA 3 cut(s) 216, 333, 369
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 3 cut(s) 28, 465, 471
TspRI CASTG 1 cut(s) 424
VneI GTGCAC 1 cut(s) 344
VpaK11BI GGWCC 1 cut(s) 386
XapI RAATTY 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 249
XmnI GAANNNNTTC 2 cut(s) 143, 462
ZraI GACGTC 2 cut(s) 49, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.