Rh5DG299500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006923
Physical Location & Seq
Reverse (-)
37633282 .. 37666905
33624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG299500.1

Sequence Viewer

Length: 495 bp
ATGCAAGCTTCGCGACCCTTGAACAATTTTCACTACCGCATTTCCGTCTCCGACAAAGATCCGGCCGAGCCCAGCCGGAAAAGAAAGGTCCGGGAGGATTATCACATGAGGGTCACGGTGTCCTGGGACGTGATGCGAACCTGCTACTGCTCCGACGATCCCACCAGGCCGATCGTTGATCTCGATTTCGAAACCGCCACTTTTCGCGATCTGGAATATCGGCTCTTCACCGTCATCGACGACGACGACGTCGGCGTCGTAGACAACATGATAACCCGAATTGGGCGTGTTGTTTATGGAGGCGTGCCGTTTGATGAGGTTAATGAGATGGTGCACCGAATACGGCGTGTCGTCGATGAAGCTTTTCGGTCTGGGCGACCAAAGGAGAAGCTGGATGTAACGGTGACGGTGAAAAACGAAACTGGGTTGCCACTCGAGGAGTACGAAGCACTGCTTTGGGCAAGCAAGAGTCAACGTCATTTAGGATTAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

19.07

Weight (kDa)

5.61

Isoelectric Point (pI)

28.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 248, 254
AatII GACGTC 1 cut(s) 252
Acc36I ACCTGC 1 cut(s) 149
AccI GTMKAC 1 cut(s) 261
AccII CGCG 2 cut(s) 13, 207
AciI CCGC 2 cut(s) 37, 195
AclWI GGATC 2 cut(s) 53, 152
AcoI YGGCCR 1 cut(s) 63
AcyI GRCGYC 2 cut(s) 249, 255
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 282
AgsI TTSAA 1 cut(s) 22
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 2 cut(s) 122, 164
AluBI AGCT 3 cut(s) 8, 362, 391
AluI AGCT 3 cut(s) 8, 362, 391
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 1 cut(s) 52
Alw44I GTGCAC 1 cut(s) 332
AlwI GGATC 2 cut(s) 53, 152
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 2 cut(s) 63, 167
ApaLI GTGCAC 1 cut(s) 332
Asp700I GAANNNNTTC 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 3 cut(s) 220, 415, 421
AsuII TTCGAA 1 cut(s) 189
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 1 cut(s) 88
BaeGI GKGCMC 1 cut(s) 336
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 336
BccI CCATC 1 cut(s) 322
BceAI ACGGC 2 cut(s) 292, 359
BciT130I CCWGG 2 cut(s) 124, 166
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 52
BfuAI ACCTGC 1 cut(s) 149
Bme1390I CCNGG 3 cut(s) 92, 124, 166
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 434
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 88
BmrFI CCNGG 3 cut(s) 92, 124, 166
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 1 cut(s) 123
BmuI ACTGGG 1 cut(s) 432
Bpu14I TTCGAA 1 cut(s) 189
BpuMI CCSGG 1 cut(s) 92
BsaHI GRCGYC 2 cut(s) 249, 255
BsaJI CCNNGG 1 cut(s) 123
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse1I ACTGG 1 cut(s) 427
BseBI CCWGG 2 cut(s) 124, 166
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 427
BseRI GAGGAG 1 cut(s) 452
BseSI GKGCMC 1 cut(s) 336
BseX3I CGGCCG 1 cut(s) 63
BseYI CCCAGC 1 cut(s) 71
Bsh1236I CGCG 2 cut(s) 13, 207
Bsh1285I CGRYCG 2 cut(s) 66, 174
BshFI GGCC 2 cut(s) 65, 169
BsiEI CGRYCG 2 cut(s) 66, 174
BsiHKAI GWGCWC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 3 cut(s) 62, 76, 91
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 65, 169
BsoBI CYCGRG 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 72, 336
Bsp143I GATC 5 cut(s) 58, 157, 171, 178, 208
Bsp68I TCGCGA 2 cut(s) 13, 207
BspACI CCGC 2 cut(s) 37, 195
BspANI GGCC 2 cut(s) 65, 169
BspFNI CGCG 2 cut(s) 13, 207
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 2 cut(s) 53, 152
BspQI GCTCTTC 1 cut(s) 230
BspT104I TTCGAA 1 cut(s) 189
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 5 cut(s) 58, 157, 171, 178, 208
BssNI GRCGYC 2 cut(s) 249, 255
Bst2UI CCWGG 2 cut(s) 124, 166
Bst4CI ACNGT 4 cut(s) 118, 232, 403, 409
Bst6I CTCTTC 1 cut(s) 230
BstACI GRCGYC 2 cut(s) 249, 255
BstBI TTCGAA 1 cut(s) 189
BstC8I GCNNGC 3 cut(s) 6, 305, 463
BstF5I GGATG 1 cut(s) 400
BstFNI CGCG 2 cut(s) 13, 207
BstKTI GATC 5 cut(s) 61, 160, 174, 181, 211
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 5 cut(s) 58, 157, 171, 178, 208
BstMCI CGRYCG 2 cut(s) 66, 174
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 2 cut(s) 124, 166
BstSCI CCNGG 3 cut(s) 90, 122, 164
BstSLI GKGCMC 1 cut(s) 336
BstUI CGCG 2 cut(s) 13, 207
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 2 cut(s) 65, 169
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 1 cut(s) 400
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 1 cut(s) 449
BtuMI TCGCGA 2 cut(s) 13, 207
BveI ACCTGC 1 cut(s) 149
Cac8I GCNNGC 3 cut(s) 6, 305, 463
Cfr13I GGNCC 1 cut(s) 88
CseI GACGC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 442
CviAII CATG 2 cut(s) 106, 268
CviJI RGCY 9 cut(s) 8, 65, 70, 75, 169, 223, 362, 391, 492
CviKI_1 RGCY 9 cut(s) 8, 65, 70, 75, 169, 223, 362, 391, 492
CviQI GTAC 1 cut(s) 442
DpnI GATC 5 cut(s) 60, 159, 173, 180, 210
DpnII GATC 5 cut(s) 58, 157, 171, 178, 208
DrdI GACNNNNNNGTC 2 cut(s) 248, 254
DseDI GACNNNNNNGTC 2 cut(s) 248, 254
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
EclXI CGGCCG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 88
Eco52I CGGCCG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 434
EcoRII CCWGG 2 cut(s) 122, 164
EcoT38I GRGCYC 1 cut(s) 72
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 2 cut(s) 109, 271
FaiI YATR 3 cut(s) 107, 269, 297
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 1 cut(s) 140
FatI CATG 2 cut(s) 105, 267
FblI GTMKAC 1 cut(s) 261
FokI GGATG 1 cut(s) 407
FriOI GRGCYC 1 cut(s) 72
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 2 cut(s) 65, 169
HapII CCGG 3 cut(s) 62, 76, 91
HgaI GACGC 1 cut(s) 244
Hin1I GRCGYC 2 cut(s) 249, 255
Hin1II CATG 2 cut(s) 109, 271
HincII GTYRAC 1 cut(s) 473
HindII GTYRAC 1 cut(s) 473
HindIII AAGCTT 2 cut(s) 6, 360
HinfI GANTC 1 cut(s) 469
HpaII CCGG 3 cut(s) 62, 76, 91
HphI GGTGA 3 cut(s) 220, 415, 421
Hpy166II GTNNAC 3 cut(s) 262, 334, 473
Hpy188I TCNGA 2 cut(s) 52, 154
Hpy188III TCNNGA 4 cut(s) 12, 182, 206, 212
Hpy8I GTNNAC 3 cut(s) 262, 334, 473
Hpy99I CGWCG 8 cut(s) 158, 242, 245, 248, 251, 254, 260, 356
HpyCH4III ACNGT 4 cut(s) 118, 232, 403, 409
HpyCH4IV ACGT 3 cut(s) 129, 249, 475
HpyCH4V TGCA 2 cut(s) 4, 334
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpySE526I ACGT 3 cut(s) 129, 249, 475
Hsp92I GRCGYC 2 cut(s) 249, 255
Hsp92II CATG 2 cut(s) 109, 271
Kzo9I GATC 5 cut(s) 58, 157, 171, 178, 208
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 1 cut(s) 155
LweI GCATC 1 cut(s) 123
MaeII ACGT 3 cut(s) 129, 249, 475
MaeIII GTNAC 3 cut(s) 112, 397, 403
MalI GATC 5 cut(s) 60, 159, 173, 180, 210
MboI GATC 5 cut(s) 58, 157, 171, 178, 208
MboII GAAGA 1 cut(s) 217
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 2 cut(s) 72, 336
MluCI AATT 2 cut(s) 25, 279
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 2 cut(s) 75, 177
MnlI CCTC 5 cut(s) 88, 102, 293, 310, 430
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 1 cut(s) 321
MspI CCGG 3 cut(s) 62, 76, 91
MspR9I CCNGG 3 cut(s) 92, 124, 166
MvaI CCWGG 2 cut(s) 124, 166
MvnI CGCG 2 cut(s) 13, 207
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 92
NdeII GATC 5 cut(s) 58, 157, 171, 178, 208
NlaIII CATG 2 cut(s) 109, 271
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 2 cut(s) 112, 403
NruI TCGCGA 2 cut(s) 13, 207
NspV TTCGAA 1 cut(s) 189
PaeR7I CTCGAG 1 cut(s) 434
PciSI GCTCTTC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 7 cut(s) 180, 243, 246, 252, 255, 373, 441
PdmI GAANNNNTTC 1 cut(s) 363
PflFI GACNNNGTC 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 90
Ple19I CGATCG 1 cut(s) 174
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
Psp6I CCWGG 2 cut(s) 122, 164
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 2 cut(s) 122, 164
PspPI GGNCC 1 cut(s) 88
PspXI VCTCGAGB 1 cut(s) 434
PsuI RGATCY 1 cut(s) 58
PsyI GACNNNGTC 1 cut(s) 248
PvuI CGATCG 1 cut(s) 174
RruI TCGCGA 2 cut(s) 13, 207
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 230
SaqAI TTAA 1 cut(s) 321
Sau3AI GATC 5 cut(s) 58, 157, 171, 178, 208
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 3 cut(s) 92, 124, 166
SduI GDGCHC 2 cut(s) 72, 336
SetI ASST 9 cut(s) 10, 90, 132, 143, 252, 321, 364, 393, 478
SfaNI GCATC 1 cut(s) 123
Sfr274I CTCGAG 1 cut(s) 434
SfuI TTCGAA 1 cut(s) 189
SinI GGWCC 1 cut(s) 88
SlaI CTCGAG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 2 cut(s) 25, 279
SsiI CCGC 2 cut(s) 37, 195
StyD4I CCNGG 3 cut(s) 90, 122, 164
TaaI ACNGT 4 cut(s) 118, 232, 403, 409
TaiI ACGT 3 cut(s) 132, 252, 478
TaqI TCGA 5 cut(s) 183, 189, 237, 354, 435
TaqII GACCGA 1 cut(s) 357
TasI AATT 2 cut(s) 25, 279
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 456
TseFI GTSAC 2 cut(s) 112, 403
Tsp45I GTSAC 2 cut(s) 112, 403
TspDTI ATGAA 1 cut(s) 372
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 1 cut(s) 456
Tth111I GACNNNGTC 1 cut(s) 248
VneI GTGCAC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 88
XhoI CTCGAG 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 363
ZraI GACGTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.