Rorug01G0203800

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006923
Physical Location & Seq
Reverse (-)
28397625 .. 28409766
12142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0203800.1

Sequence Viewer

Length: 4101 bp
ATGGCTCCTAGAAAGAAACCCAATTCTTCTTCTTCTACTACCGCTTCGTCTCGGAAATCGAACTCGAAAGCTCAGCCTCAGCCTTCCAAGTTCAGCATCCAACATTTCTTTGAGCGCCACACCCAGAACTCTCAAAACCCCCAAACCACCGCCTCTTCCGCTCCTCACAACCCTAAGCCCCCAATCCCCGACTCTGCCACCGCCATTGTTCCGCAGAAGTCGATTCCGGAGATCCAAAACCCTGCCCCGATTGAACCAACTAGTGCACTAGTAGCTTCTGGAAATCCCGAGAATCGGCGTGATTCGCAGTCGAGTAGTAAGAACAGTACGCAGGAGACACCAACTGAGAATTTGGTGGCGGTGAGGTTTATGGATGCCGAGCCGAATGTGGATGAGGAGGTCTCACCGGAGGTTTCGAAGTCTAAGTCTGTCAAGCGATTCAAGTTCTCCCCTGGAATGTTGATAAAGCAGAGTCAAGATGATGGAGGTGATGAGGTGACGTGGAGGACGTCTCCGGTCAATGAAAGACTTAAAGCAGTTTCAAAGCACAAGCCTGAGATGATGAGAGTGTTGGCAGACTCTTCAAGGCTGAAATCTTTAAGCATTAACCAATGCTCGCAAAACAAGACTTCTGCTGTTGAAGCTGGAAAGGTTGAGAAATCCCTGTCGTTGGGGTCCCTGAATAGGGTTGGCGTGAAGAGGGTTAAACCGGATCAGGATGTGGAACATAATGGAAATGTGGTCGATAGGACTATTTCTGAAATAGCTGGTAGTCAGAGTCCATTCCGAACTCCACCTTCTTTATCATACTGTCATGATAAGCTTGCTAATGCTCTTTCATGTGATGGAGTTTCTGATGAGCCAGGGTTGAGGCCGCATAAAAAGGCATTGCTTGAACTCTTAGATCAAGTAGAAGATGTTATTTTTGTTGAAGATTCAGTACCTGATGATGTGGAGGCATCTCAATTACAAGTTAAAGATAAATCAGAGCATGTCGTAAAAATGGTGACAGCTCCAACAGAACAGGTTACTCGGGCATCTTCCAATTACAGTTTCCTTGTATTGGAGGTATCTGAGAATTGTAGACCTGATGATTCCTCTGGTGCTCAGTGCATGTATAAGGTTCTACGCTTATTGAATGAGCAAAGTGGAGAAGAGTGTGCTGTGTATTTACGGGAAGAGTGGTTTTACAGTGTCATTGCACCTGGAGATACTGTCAACGTTATTGGTGAATTTGATGACCAAGGACAGTGTCATGTGGATCATCACCATAACTTTTTAATTGTTCACCCCGATGTCTTAGTGTCTGGAACTCGGGTTGCAGCTAATTTCATTTGTCCTAGGCGAACTGTGCTGGATGAAAGGCTAAAAGGCAATGAGTATTCAACTCCAGCACTAATTGGCACAATATTGCATCAAGTTTTTGAGGCTGCACTTATGAAGGAGAAACCCACAATTACTTTCTTGGAGGAACATGCAAGAATGGGGCTCCAGAGAAACATTGAGAACTTGTATGCATGTGGAGTTCATGAAAATGATGTATACAAAACGTTGGTTGATGCTGTCCCAAAAATATTAAATTGGGTGAATGTCTTCAAAAACACCCAGGATTCTGAAGCTTCATCTGTTGATTTTGGATCTGAGAATGGGGTGAAAAAAGTCACAATATCTGAGGTAATTGATATTGAGGAGATGGCATGGGCTCCAAAATATGGTCTAAAAGGTATGATTGATGCTTCTGTCAGGGTGAAAGTCGAAGCTAACAAAAATGAATCTGACATGAAGGTTATGCCTTTGGAGTTTAAAACCGGGAAAGTGCCGAAGGACCAGGAGCATTGTGCCCAAGTAATCTTATACACTCTTCTTATGTCGGAGAGGTACCAGAAGCATGTTGATACTGGTCTTCTATGTTACCTCCAGTCTGATCAGACACAGGGAATTGCGGTTAGAAGATCTGACCTTGTTGGGCTAATCATGCAACGTAATCAACTTGCAAATGATATTGTCAAGGCCTCAAGACTGCAAGTCCTTCCCCCAATGTTACGGAGTCCAAGCTTGTGCAGAAGTTGTCGCCATCTGAATGTGTGCACCATCTATCACAAGGCACATGGAGGCAACGCAGATAGCAGCGGATTGGGAGATTTATTTGATTCAAATACTCACCAGCTAACAACTGCTCATGGTGTTTTCCTAAGGCTTTGGGACTGGTTGATTGATTTAGAAGCTAAAGAGATGGAGCTTGTAAAGAAAGAAATGTGGCGGCCACATGGTTTGAAGAGTGATCATTCCACAAGTTGCCTTTGTTCTATGGTTCTTGATAATGAACTTCCATATTCAAGGTCTGGAAGTGATAACAAATTCATTTACCGTTTTCTGCGTACTGGTTTGTCTTCTCTCAATGTGAGGACATCCAATGGAGATTTTCAGAATGCTGCTTCTTCACCCTCAAAATTTGCGGATTGCACACTAAGATGTGGTGACCATGTGATAATTAGCAGTGAATCTGGTCATCTGGCTGTTGCAAGGGGGGTCATAACAGATATAAGTCACTCCCATGTTTCTGTTTCTTTTTCTAAGAAGTTACGACTTCCTGGGGGCAATCCTTCTTCAGAGGCAAGTGATCTTCGTCGGGAGGTCTGGCGGGTTGACAAAGATGAATTTATGACATCATTTGCCACAATGAGGTTCAACCTTCTTCAACTTTTTCTTCCGAGTGCCCAAACTGCTCATCTGAGAAAAATGATCGTTGACCTAGAGGCTCCTAGATTTGACAGTGGATGTATTACGTTCAGCCAGGATCCAGCAATATCCTATGTCTGGTCCCAAAGAAATTTAAATGATGATCAGCGTAGTGCCATTCTAAAGATACTTACAGCGAAGGACTACGCTCTTATACTAGGAATGCCTGGAACAGGCAAGACATCTACTATGGTGCATGCTGTGAAAGCCTTGTTGATGCGAGGTGCATCAATTTTGCTTACCTCCTACACAAATTCTGCTGTTGATAATCTACTTATCAAATTAAAAGCTCAGAATATAGACTTTGTGCGCATTGGAAGACACGAAGTTGTGCATGAAGAAGTTCGAGGGCATTGCTTTTCAGCAATGAACATACAAAGTGTGGAAGACATTAAGTCAAGATTGGACCAAGTCAAGGTGGTTGCTGTTACATGCTTGGGGATCACTAGTCCCTTGCTTGCCAACAAGAAATTTGATGTATGCATCATGGATGAAGCTGGACAGACCACCCTTCCAGTATCTCTCGGGCCTTTGATGTTTGCTTCAATATTTGTCCTGGTTGGTGATCATTATCAATTACCTCCTCTGGTGAAGAGTACAGAGGCTCGAGAAAATGGAATGGGGGTAAGCTTGTTTTGCAGGCTCTCAGAAGCACATCCTCAGGCAATTTCAGCATTGCAAAGCCAGTACCGTATGTGTCAAGGCATTATGGAACTGTCAAATGCCTTGATATATGGTGACAGATTGCGTTGTGGATCTCCTGAAATAGCCAATGCCAAACTCAACTTTTCAAGTTCGAAGTCTCATTCATTGTGGATAAAGGAGGTTTTAAATCCAAGCAAACCAGTTATATTTATTAATACAGATGCTTTACCTGCTTTCGAGGCAAAGGACCACAAGATTGTGAATAACCCGATGGAAGCTCACATAGTCGTAGAGGTCGTGGAGGAATTAGTTAACAGTGGAATTGAAGGGGATGATATTGGCATCATTACACCGTATAACTCCCAGGCAGAGATCATTCGGCTTGCCATCAACCTTACTTCCGTGGAGATACATACCATTGACAAATACCAGGGAAGAGATAAAGATTGCATACTAGTCTCCTTTGTTAGGTCAACCGAAAATCCACGAAACTGCACGTCCTCTCTGCTCGGAGACTGGCATAGGATTAATGTGGCTTTAACTCGTGCCAAGAAAAAGTTGATAATGGTCGGGTCACGCGAAACCCTATCCAAAGTTCTGCTGATGAAGCTTCTTATCGAGAAAGTTGAGGAACAATCAGGCATCTTCAACGTGTCCAAGAAAGACATTAACTTTAAGAGGGAGCTCAAGAGATGCTCTCAGGTCAGATCATTGTAA

Protein Analysis

1366

Amino Acids

151.66

Weight (kDa)

6.91

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 381 - 583 1.4e-63 DNA replication factor Dna2
AAA_11 PF13086 944 - 1035 3.1e-16 AAA domain
AAA_30 PF13604 945 - 1108 3.4e-09 AAA domain
AAA_19 PF13245 949 - 1108 5.1e-07 AAA domain
AAA_11 PF13086 1044 - 1112 5.9e-16 AAA domain
AAA_12 PF13087 1121 - 1321 8e-54 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 512
Acc16I TGCGCA 1 cut(s) 3050
Acc36I ACCTGC 1 cut(s) 3622
Acc65I GGTACC 1 cut(s) 1878
AccB1I GGYRCC 1 cut(s) 1878
AccB7I CCANNNNNTGG 1 cut(s) 1712
AccBSI CCGCTC 1 cut(s) 161
AccI GTMKAC 2 cut(s) 1084, 1542
AccII CGCG 1 cut(s) 3963
AccIII TCCGGA 1 cut(s) 226
AclI AACGTT 2 cut(s) 1221, 1550
AclWI GGATC 8 cut(s) 226, 720, 1269, 1645, 2792, 2805, 3188, 3502
AcoI YGGCCR 1 cut(s) 2261
AcsI RAATTY 8 cut(s) 349, 1232, 2357, 2450, 2657, 2830, 2992, 3209
AcuI CTGAAG 2 cut(s) 1635, 2592
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 6 cut(s) 328, 942, 1880, 2380, 3337, 3428
AflIII ACRYGT 1 cut(s) 4035
AhdI GACNNNNNGTC 2 cut(s) 2024, 3133
AhlI ACTAGT 4 cut(s) 260, 268, 3185, 3838
AjiI CACGTC 2 cut(s) 501, 3882
AjuI GAANNNNNNNTTGG 2 cut(s) 1037, 1069
Alw21I GWGCWC 4 cut(s) 268, 1108, 2090, 4071
Alw26I GTCTC 7 cut(s) 54, 329, 406, 516, 3546, 3847, 3891
Alw44I GTGCAC 2 cut(s) 264, 2086
AlwI GGATC 8 cut(s) 226, 720, 1269, 1645, 2792, 2805, 3188, 3502
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 5 cut(s) 287, 1032, 1314, 3263, 3345
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 4 cut(s) 872, 2010, 2261, 3266
ApaLI GTGCAC 2 cut(s) 264, 2086
ApeKI GCWGC 4 cut(s) 1322, 1430, 2127, 2432
ApoI RAATTY 8 cut(s) 349, 1232, 2357, 2450, 2657, 2830, 2992, 3209
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AseI ATTAAT 2 cut(s) 3597, 3912
Asp700I GAANNNNTTC 2 cut(s) 1592, 3081
Asp718I GGTACC 1 cut(s) 1878
AspA2I CCTAGG 1 cut(s) 1340
AspLEI GCGC 2 cut(s) 117, 3051
AspS9I GGNCC 6 cut(s) 675, 1825, 2820, 3145, 3266, 3631
AsuC2I CCSGG 1 cut(s) 1810
AsuII TTCGAA 2 cut(s) 416, 3536
AvaI CYCGRG 5 cut(s) 287, 1032, 1314, 3263, 3345
AvaII GGWCC 5 cut(s) 675, 1825, 2820, 3145, 3631
AvrII CCTAGG 1 cut(s) 1340
AxyI CCTNAGG 2 cut(s) 2192, 3399
BaeGI GKGCMC 4 cut(s) 268, 1843, 2090, 2719
BamHI GGATCC 1 cut(s) 2797
BanI GGYRCC 1 cut(s) 1878
BanII GRGCYC 3 cut(s) 1491, 1705, 4071
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BauI CACGAG 1 cut(s) 3927
BbsI GAAGAC 5 cut(s) 1585, 1895, 2382, 3064, 3132
Bbv12I GWGCWC 4 cut(s) 268, 1108, 2090, 4071
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 4 cut(s) 1334, 1417, 2139, 2419
BccI CCATC 8 cut(s) 476, 839, 1687, 2082, 2099, 2227, 3649, 3779
BcgI CGANNNNNNTGC 2 cut(s) 3075, 3109
BclI TGATCA 4 cut(s) 1924, 2281, 2842, 3304
BcnI CCSGG 1 cut(s) 1810
BcoDI GTCTC 7 cut(s) 54, 329, 406, 516, 3546, 3847, 3891
BcuI ACTAGT 4 cut(s) 260, 268, 3185, 3838
BfaI CTAG 9 cut(s) 9, 261, 269, 1341, 2753, 2763, 2897, 3186, 3839
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 3622
BglII AGATCT 1 cut(s) 1952
BisI GCNGC 6 cut(s) 875, 1323, 1431, 2128, 2261, 2433
BlnI CCTAGG 1 cut(s) 1340
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 6 cut(s) 876, 1324, 1432, 2129, 2262, 2434
Bme18I GGWCC 5 cut(s) 675, 1825, 2820, 3145, 3631
BmeRI GACNNNNNGTC 2 cut(s) 2024, 3133
BmeT110I CYCGRG 5 cut(s) 287, 1032, 1314, 3263, 3345
BmgBI CACGTC 2 cut(s) 501, 3882
BmgT120I GGNCC 6 cut(s) 675, 1825, 2820, 3145, 3266, 3631
BmiI GGNNCC 9 cut(s) 6, 676, 677, 1490, 1704, 1880, 2760, 2799, 2822
BpiI GAAGAC 5 cut(s) 1585, 1895, 2382, 3064, 3132
BplI GAGNNNNNCTC 6 cut(s) 386, 418, 496, 528, 4066, 4098
BpmI CTGGAG 4 cut(s) 1227, 1374, 1475, 1901
Bpu10I CCTNAGC 2 cut(s) 78, 174
Bpu1102I GCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 2 cut(s) 416, 3536
BpuEI CTTGAG 2 cut(s) 1999, 4055
BpuMI CCSGG 1 cut(s) 1810
BsaBI GATNNNNATC 1 cut(s) 3309
BsaHI GRCGYC 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 9 cut(s) 451, 863, 1243, 1340, 1605, 2591, 3747, 3786, 3814
BsaWI WCCGGW 4 cut(s) 226, 406, 514, 709
BsaXI ACNNNNNCTCC 2 cut(s) 1200, 1230
Bse1I ACTGG 8 cut(s) 1903, 1918, 2210, 2386, 3254, 3424, 3584, 3905
Bse21I CCTNAGG 2 cut(s) 2192, 3399
Bse3DI GCAATG 6 cut(s) 887, 1197, 1381, 3091, 3111, 3413
Bse8I GATNNNNATC 1 cut(s) 3309
BseAI TCCGGA 1 cut(s) 226
BseDI CCNNGG 9 cut(s) 451, 863, 1243, 1340, 1605, 2591, 3747, 3786, 3814
BseJI GATNNNNATC 1 cut(s) 3309
BseMI GCAATG 6 cut(s) 887, 1197, 1381, 3091, 3111, 3413
BseNI ACTGG 8 cut(s) 1903, 1918, 2210, 2386, 3254, 3424, 3584, 3905
BseRI GAGGAG 4 cut(s) 153, 410, 1703, 3312
BseSI GKGCMC 4 cut(s) 268, 1843, 2090, 2719
BseXI GCAGC 4 cut(s) 1334, 1417, 2139, 2419
BsgI GTGCAG 3 cut(s) 1416, 2080, 3862
Bsh1236I CGCG 1 cut(s) 3963
BshFI GGCC 4 cut(s) 874, 2012, 2263, 3268
BshNI GGYRCC 1 cut(s) 1878
BsiHKAI GWGCWC 4 cut(s) 268, 1108, 2090, 4071
BsiHKCI CYCGRG 5 cut(s) 287, 1032, 1314, 3263, 3345
BsiSI CCGG 5 cut(s) 227, 407, 515, 710, 1809
BslFI GGGAC 5 cut(s) 661, 1550, 2216, 2806, 3174
BsmAI GTCTC 7 cut(s) 54, 329, 406, 516, 3546, 3847, 3891
BsmBI CGTCTC 2 cut(s) 54, 516
BsmFI GGGAC 5 cut(s) 661, 1550, 2216, 2806, 3174
BsmI GAATGC 2 cut(s) 2434, 2907
BsnI GGCC 4 cut(s) 874, 2012, 2263, 3268
Bso31I GGTCTC 1 cut(s) 406
BsoBI CYCGRG 5 cut(s) 287, 1032, 1314, 3263, 3345
Bsp119I TTCGAA 2 cut(s) 416, 3536
Bsp1286I GDGCHC 8 cut(s) 268, 1108, 1491, 1705, 1843, 2090, 2719, 4071
Bsp13I TCCGGA 1 cut(s) 226
Bsp1720I GCTNAGC 1 cut(s) 72
BspANI GGCC 4 cut(s) 874, 2012, 2263, 3268
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 1 cut(s) 3963
BspHI TCATGA 2 cut(s) 814, 1528
BspLI GGNNCC 9 cut(s) 6, 676, 677, 1490, 1704, 1880, 2760, 2799, 2822
BspMI ACCTGC 1 cut(s) 3622
BspPI GGATC 8 cut(s) 226, 720, 1269, 1645, 2792, 2805, 3188, 3502
BspT104I TTCGAA 2 cut(s) 416, 3536
BspT107I GGYRCC 1 cut(s) 1878
BspTNI GGTCTC 1 cut(s) 406
BsrBI CCGCTC 1 cut(s) 161
BsrDI GCAATG 6 cut(s) 887, 1197, 1381, 3091, 3111, 3413
BsrI ACTGG 8 cut(s) 1903, 1918, 2210, 2386, 3254, 3424, 3584, 3905
BssECI CCNNGG 9 cut(s) 451, 863, 1243, 1340, 1605, 2591, 3747, 3786, 3814
BssNAI GTATAC 1 cut(s) 1543
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 1 cut(s) 3927
BssT1I CCWWGG 2 cut(s) 1243, 1340
Bst1107I GTATAC 1 cut(s) 1543
Bst2BI CACGAG 1 cut(s) 3927
Bst6I CTCTTC 9 cut(s) 160, 586, 692, 1149, 1173, 1866, 2270, 3326, 3814
BstACI GRCGYC 1 cut(s) 509
BstBI TTCGAA 2 cut(s) 416, 3536
BstC8I GCNNGC 6 cut(s) 617, 825, 2937, 3198, 3380, 3768
BstDSI CCRYGG 1 cut(s) 3786
BstEII GGTNACC 1 cut(s) 2477
BstENI CCTNNNNNAGG 2 cut(s) 2910, 3850
BstFNI CGCG 1 cut(s) 3963
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 117, 3051
BstMAI GTCTC 7 cut(s) 54, 329, 406, 516, 3546, 3847, 3891
BstNSI RCATGY 7 cut(s) 995, 1117, 1478, 1521, 1892, 2939, 3174
BstPI GGTNACC 1 cut(s) 2477
BstSLI GKGCMC 4 cut(s) 268, 1843, 2090, 2719
BstUI CGCG 1 cut(s) 3963
BstV1I GCAGC 4 cut(s) 1334, 1417, 2139, 2419
BstV2I GAAGAC 5 cut(s) 1585, 1895, 2382, 3064, 3132
BstX2I RGATCY 5 cut(s) 231, 1637, 1952, 2797, 3494
BstYI RGATCY 5 cut(s) 231, 1637, 1952, 2797, 3494
BstZ17I GTATAC 1 cut(s) 1543
Bsu36I CCTNAGG 2 cut(s) 2192, 3399
BsuRI GGCC 4 cut(s) 874, 2012, 2263, 3268
BtgI CCRYGG 1 cut(s) 3786
BtrI CACGTC 2 cut(s) 501, 3882
BtsI GCAGTG 1 cut(s) 2503
BtsIMutI CAGTG 6 cut(s) 1115, 1198, 1256, 2503, 2779, 3706
BveI ACCTGC 1 cut(s) 3622
Cac8I GCNNGC 6 cut(s) 617, 825, 2937, 3198, 3380, 3768
CaiI CAGNNNCTG 1 cut(s) 944
CciI TCATGA 2 cut(s) 814, 1528
CfoI GCGC 2 cut(s) 117, 3051
Cfr13I GGNCC 6 cut(s) 675, 1825, 2820, 3145, 3266, 3631
Csp6I GTAC 6 cut(s) 327, 941, 1879, 2379, 3336, 3427
CspCI CAANNNNNGTGG 4 cut(s) 187, 222, 2253, 2288
CviQI GTAC 6 cut(s) 327, 941, 1879, 2379, 3336, 3427
DraI TTTAAA 3 cut(s) 1804, 2835, 3570
DriI GACNNNNNGTC 2 cut(s) 2024, 3133
EaeI YGGCCR 1 cut(s) 2261
Eam1104I CTCTTC 9 cut(s) 160, 586, 692, 1149, 1173, 1866, 2270, 3326, 3814
Eam1105I GACNNNNNGTC 2 cut(s) 2024, 3133
EarI CTCTTC 9 cut(s) 160, 586, 692, 1149, 1173, 1866, 2270, 3326, 3814
Ecl136II GAGCTC 1 cut(s) 4069
Eco130I CCWWGG 2 cut(s) 1243, 1340
Eco147I AGGCCT 1 cut(s) 2012
Eco24I GRGCYC 3 cut(s) 1491, 1705, 4071
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 5 cut(s) 675, 1825, 2820, 3145, 3631
Eco53kI GAGCTC 1 cut(s) 4069
Eco57I CTGAAG 2 cut(s) 1635, 2592
Eco81I CCTNAGG 2 cut(s) 2192, 3399
Eco88I CYCGRG 5 cut(s) 287, 1032, 1314, 3263, 3345
Eco91I GGTNACC 1 cut(s) 2477
EcoICRI GAGCTC 1 cut(s) 4069
EcoNI CCTNNNNNAGG 2 cut(s) 2910, 3850
EcoO109I RGGNCCY 1 cut(s) 675
EcoO65I GGTNACC 1 cut(s) 2477
EcoT14I CCWWGG 2 cut(s) 1243, 1340
EcoT22I ATGCAT 2 cut(s) 1519, 3224
EcoT38I GRGCYC 3 cut(s) 1491, 1705, 4071
ErhI CCWWGG 2 cut(s) 1243, 1340
Esp3I CGTCTC 2 cut(s) 54, 516
FaqI GGGAC 5 cut(s) 661, 1550, 2216, 2806, 3174
FauI CCCGC 1 cut(s) 2634
FbaI TGATCA 4 cut(s) 1924, 2281, 2842, 3304
FblI GTMKAC 2 cut(s) 1084, 1542
Fnu4HI GCNGC 6 cut(s) 875, 1323, 1431, 2128, 2261, 2433
FriOI GRGCYC 3 cut(s) 1491, 1705, 4071
Fsp4HI GCNGC 6 cut(s) 875, 1323, 1431, 2128, 2261, 2433
FspAI RTGCGCAY 1 cut(s) 3050
FspBI CTAG 9 cut(s) 9, 261, 269, 1341, 2753, 2763, 2897, 3186, 3839
FspI TGCGCA 1 cut(s) 3050
GlaI GCGC 2 cut(s) 116, 3050
GluI GCNGC 6 cut(s) 875, 1323, 1431, 2128, 2261, 2433
GsuI CTGGAG 4 cut(s) 1227, 1374, 1475, 1901
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 4 cut(s) 874, 2012, 2263, 3268
HapII CCGG 5 cut(s) 227, 407, 515, 710, 1809
HhaI GCGC 2 cut(s) 117, 3051
Hin1I GRCGYC 1 cut(s) 509
Hin6I GCGC 2 cut(s) 115, 3049
HinP1I GCGC 2 cut(s) 115, 3049
HincII GTYRAC 5 cut(s) 1219, 2647, 2749, 3697, 3858
HindII GTYRAC 5 cut(s) 1219, 2647, 2749, 3697, 3858
HindIII AAGCTT 5 cut(s) 821, 1617, 2053, 3367, 3992
HpaI GTTAAC 1 cut(s) 3697
HpaII CCGG 5 cut(s) 227, 407, 515, 710, 1809
Hpy99I CGWCG 1 cut(s) 2631
HpyCH4IV ACGT 8 cut(s) 500, 509, 1221, 1550, 1981, 2786, 3881, 4035
HpySE526I ACGT 8 cut(s) 500, 509, 1221, 1550, 1981, 2786, 3881, 4035
Hsp92I GRCGYC 1 cut(s) 509
HspAI GCGC 2 cut(s) 115, 3049
KflI GGGWCCC 1 cut(s) 675
Kpn2I TCCGGA 1 cut(s) 226
KpnI GGTACC 1 cut(s) 1882
Ksp22I TGATCA 4 cut(s) 1924, 2281, 2842, 3304
KspAI GTTAAC 1 cut(s) 3697
LmnI GCTCC 9 cut(s) 10, 166, 1018, 1494, 1708, 1831, 2236, 2764, 4066
Lsp1109I GCAGC 4 cut(s) 1334, 1417, 2139, 2419
MaeI CTAG 9 cut(s) 9, 261, 269, 1341, 2753, 2763, 2897, 3186, 3839
MaeII ACGT 8 cut(s) 500, 509, 1221, 1550, 1981, 2786, 3881, 4035
MbiI CCGCTC 1 cut(s) 161
MflI RGATCY 5 cut(s) 231, 1637, 1952, 2797, 3494
MhlI GDGCHC 8 cut(s) 268, 1108, 1491, 1705, 1843, 2090, 2719, 4071
MlyI GAGTC 5 cut(s) 185, 481, 572, 787, 2056
MmeI TCCRAC 3 cut(s) 124, 1040, 1851
Mph1103I ATGCAT 2 cut(s) 1519, 3224
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 2 cut(s) 1592, 3081
MslI CAYNNNNRTG 5 cut(s) 1293, 1533, 2079, 2470, 2553
MspA1I CMGCKG 1 cut(s) 2130
MspI CCGG 5 cut(s) 227, 407, 515, 710, 1809
Mva1269I GAATGC 2 cut(s) 2434, 2907
MvnI CGCG 1 cut(s) 3963
NciI CCSGG 1 cut(s) 1810
NlaIV GGNNCC 9 cut(s) 6, 676, 677, 1490, 1704, 1880, 2760, 2799, 2822
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 7 cut(s) 496, 1006, 1660, 2477, 2546, 3476, 3957
NsbI TGCGCA 1 cut(s) 3050
NsiI ATGCAT 2 cut(s) 1519, 3224
NspI RCATGY 7 cut(s) 995, 1117, 1478, 1521, 1892, 2939, 3174
NspV TTCGAA 2 cut(s) 416, 3536
PaeI GCATGC 1 cut(s) 2939
PaeR7I CTCGAG 1 cut(s) 3345
PagI TCATGA 2 cut(s) 814, 1528
PceI AGGCCT 1 cut(s) 2012
PcsI WCGNNNNNNNCGW 3 cut(s) 506, 3678, 3960
PctI GAATGC 2 cut(s) 2434, 2907
PdmI GAANNNNTTC 2 cut(s) 1592, 3081
PflFI GACNNNGTC 2 cut(s) 1251, 3149
PflMI CCANNNNNTGG 1 cut(s) 1712
PkrI GCNGC 6 cut(s) 876, 1324, 1432, 2129, 2262, 2434
PleI GAGTC 5 cut(s) 185, 480, 572, 786, 2055
PpsI GAGTC 5 cut(s) 185, 480, 572, 786, 2055
PpuMI RGGWCCY 1 cut(s) 675
PshBI ATTAAT 2 cut(s) 3597, 3912
Psp124BI GAGCTC 1 cut(s) 4071
Psp1406I AACGTT 2 cut(s) 1221, 1550
Psp5II RGGWCCY 1 cut(s) 675
PspEI GGTNACC 1 cut(s) 2477
PspN4I GGNNCC 9 cut(s) 6, 676, 677, 1490, 1704, 1880, 2760, 2799, 2822
PspPI GGNCC 6 cut(s) 675, 1825, 2820, 3145, 3266, 3631
PspPPI RGGWCCY 1 cut(s) 675
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 5 cut(s) 231, 1637, 1952, 2797, 3494
PsyI GACNNNGTC 2 cut(s) 1251, 3149
RsaI GTAC 6 cut(s) 328, 942, 1880, 2380, 3337, 3428
RsaNI GTAC 6 cut(s) 327, 941, 1879, 2379, 3336, 3427
RseI CAYNNNNRTG 5 cut(s) 1293, 1533, 2079, 2470, 2553
SacI GAGCTC 1 cut(s) 4071
SatI GCNGC 6 cut(s) 875, 1323, 1431, 2128, 2261, 2433
Sau96I GGNCC 6 cut(s) 675, 1825, 2820, 3145, 3266, 3631
SchI GAGTC 5 cut(s) 185, 481, 572, 787, 2056
SduI GDGCHC 8 cut(s) 268, 1108, 1491, 1705, 1843, 2090, 2719, 4071
Sfr274I CTCGAG 1 cut(s) 3345
SfuI TTCGAA 2 cut(s) 416, 3536
SinI GGWCC 5 cut(s) 675, 1825, 2820, 3145, 3631
SlaI CTCGAG 1 cut(s) 3345
SmiI ATTTAAAT 1 cut(s) 2835
SmiMI CAYNNNNRTG 5 cut(s) 1293, 1533, 2079, 2470, 2553
SmlI CTYRAG 3 cut(s) 2014, 3345, 4070
SmoI CTYRAG 3 cut(s) 2014, 3345, 4070
SpeI ACTAGT 4 cut(s) 260, 268, 3185, 3838
SphI GCATGC 1 cut(s) 2939
SseBI AGGCCT 1 cut(s) 2012
SspI AATATT 3 cut(s) 1410, 1575, 3288
SspMI CTAG 9 cut(s) 9, 261, 269, 1341, 2753, 2763, 2897, 3186, 3839
SstI GAGCTC 1 cut(s) 4071
StuI AGGCCT 1 cut(s) 2012
StyI CCWWGG 2 cut(s) 1243, 1340
SwaI ATTTAAAT 1 cut(s) 2835
TaiI ACGT 8 cut(s) 503, 512, 1224, 1553, 1984, 2789, 3884, 4038
TatI WGTACW 1 cut(s) 3335
TauI GCSGC 2 cut(s) 877, 2263
TscAI CASTG 6 cut(s) 1115, 1198, 1256, 2503, 2779, 3706
TseFI GTSAC 7 cut(s) 496, 1006, 1660, 2477, 2546, 3476, 3957
TseI GCWGC 4 cut(s) 1322, 1430, 2127, 2432
Tsp45I GTSAC 7 cut(s) 496, 1006, 1660, 2477, 2546, 3476, 3957
TspGWI ACGGA 2 cut(s) 2059, 3775
TspRI CASTG 6 cut(s) 1115, 1198, 1256, 2503, 2779, 3706
Tth111I GACNNNGTC 2 cut(s) 1251, 3149
Van91I CCANNNNNTGG 1 cut(s) 1712
VneI GTGCAC 2 cut(s) 264, 2086
VpaK11BI GGWCC 5 cut(s) 675, 1825, 2820, 3145, 3631
VspI ATTAAT 2 cut(s) 3597, 3912
XagI CCTNNNNNAGG 2 cut(s) 2910, 3850
XapI RAATTY 8 cut(s) 349, 1232, 2357, 2450, 2657, 2830, 2992, 3209
XceI RCATGY 7 cut(s) 995, 1117, 1478, 1521, 1892, 2939, 3174
XhoI CTCGAG 1 cut(s) 3345
XmaJI CCTAGG 1 cut(s) 1340
XmiI GTMKAC 2 cut(s) 1084, 1542
XmnI GAANNNNTTC 2 cut(s) 1592, 3081
XspI CTAG 9 cut(s) 9, 261, 269, 1341, 2753, 2763, 2897, 3186, 3839
ZraI GACGTC 1 cut(s) 510
Zsp2I ATGCAT 2 cut(s) 1519, 3224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.