FvH4_5g03690

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008133
Physical Location & Seq
Reverse (-)
2161060 .. 2161580
521 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03690.t1

Sequence Viewer

Length: 492 bp
ATGGCTAGTGCAACACCAATTTTGGCGCTTTCCCTCTTCTTCCTCCTCATATCTGGCTCCGCGGCCACAGCGACCGGCAAGAGAAGGATGGTGTTTTACGGCAATGAGAACCATACTGGCAAAGTGGCAAGAGAAAACCATTTGGGTGGCATTCTCACCTTCGATGACCCCATTAACTTGGACAACAATGTGCATTCAGCCACACTTCATCGTGCACAAAGCTTCTCTTTCTATGGCAAGAGAGAGATATTCGCTGCTTGGCTAAGTTGGCTTGGCTTCTCCTTCGTGTTCAACAACACCAAAAACACTGGAGGAAGCTTCAACTTTTGTGGTGTTGATCATTTGATGGGCAAGGCCAGGGACATTTCAGTGATTGAAGGTACTGGTGATTTCTTCATAGCTCGAGGGATAGCAACTTTGATGAGTAATACATTTCAGGGAAAGTGTACTTCCGGCTTCGCATTGATTTTAATCTGTATTGAGTGTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

164

Amino Acids

17.82

Weight (kDa)

8.43

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 36 - 146 1.8e-18 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 62
AciI CCGC 2 cut(s) 60, 62
AcoI YGGCCR 1 cut(s) 63
AfaI GTAC 2 cut(s) 382, 448
AgsI TTSAA 3 cut(s) 292, 322, 377
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 3 cut(s) 222, 318, 401
AluI AGCT 3 cut(s) 222, 318, 401
Alw21I GWGCWC 1 cut(s) 217
Alw44I GTGCAC 1 cut(s) 213
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 2 cut(s) 63, 354
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 254
AspLEI GCGC 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 148, 398
AvaI CYCGRG 1 cut(s) 402
BaeGI GKGCMC 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 241
BccI CCATC 2 cut(s) 82, 340
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 1 cut(s) 358
BclI TGATCA 1 cut(s) 337
BfaI CTAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 2 cut(s) 63, 255
BlsI GCNGC 2 cut(s) 64, 256
Bme1390I CCNGG 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 402
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 470
BsaJI CCNNGG 2 cut(s) 60, 357
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 121, 313, 388
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 470
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 2 cut(s) 60, 357
BseGI GGATG 1 cut(s) 93
BseJI GATNNNNATC 1 cut(s) 470
BseMI GCAATG 1 cut(s) 109
BseNI ACTGG 3 cut(s) 121, 313, 388
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 65, 356
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 2 cut(s) 75, 453
BslFI GGGAC 1 cut(s) 374
BsmFI GGGAC 1 cut(s) 374
BsmI GAATGC 2 cut(s) 150, 193
BsnI GGCC 2 cut(s) 65, 356
BsoBI CYCGRG 1 cut(s) 402
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 1 cut(s) 337
BspACI CCGC 2 cut(s) 60, 62
BspANI GGCC 2 cut(s) 65, 356
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 3 cut(s) 121, 313, 388
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 60, 357
BssMI GATC 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 358
Bst6I CTCTTC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 263
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 1 cut(s) 340
BstMBI GATC 1 cut(s) 337
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 2 cut(s) 68, 268
BstNI CCWGG 1 cut(s) 358
BstSCI CCNGG 1 cut(s) 356
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 1 cut(s) 241
BstXI CCANNNNNNTGG 2 cut(s) 146, 178
BsuRI GGCC 2 cut(s) 65, 356
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 306, 375
CfoI GCGC 1 cut(s) 28
Cfr10I RCCGGY 1 cut(s) 74
Cfr42I CCGCGG 1 cut(s) 63
Csp6I GTAC 2 cut(s) 381, 447
CspCI CAANNNNNGTGG 2 cut(s) 310, 345
CviQI GTAC 2 cut(s) 381, 447
DdeI CTNAG 1 cut(s) 263
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 402
EcoRII CCWGG 1 cut(s) 356
FaiI YATR 4 cut(s) 50, 114, 234, 398
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FaqI GGGAC 1 cut(s) 374
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 2 cut(s) 63, 255
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 2 cut(s) 63, 255
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 27
GluI GCNGC 2 cut(s) 63, 255
GsuI CTGGAG 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 2 cut(s) 65, 356
HapII CCGG 2 cut(s) 75, 453
HhaI GCGC 1 cut(s) 28
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HindIII AAGCTT 2 cut(s) 220, 316
HpaII CCGG 2 cut(s) 75, 453
HphI GGTGA 2 cut(s) 148, 398
Hpy166II GTNNAC 2 cut(s) 215, 447
Hpy8I GTNNAC 2 cut(s) 215, 447
HpyAV CCTTC 4 cut(s) 78, 169, 292, 371
HpyCH4V TGCA 3 cut(s) 11, 193, 215
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 268
HpyF3I CTNAG 1 cut(s) 263
HspAI GCGC 1 cut(s) 26
Ksp22I TGATCA 1 cut(s) 337
KspI CCGCGG 1 cut(s) 63
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 9 cut(s) 39, 88, 102, 294, 343, 369, 370, 422, 466
Lsp1109I GCAGC 1 cut(s) 241
MaeI CTAG 1 cut(s) 6
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 3 cut(s) 28, 31, 385
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 1 cut(s) 18
MnlI CCTC 5 cut(s) 44, 53, 56, 305, 398
MseI TTAA 2 cut(s) 174, 470
MslI CAYNNNNRTG 2 cut(s) 144, 368
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 2 cut(s) 75, 453
MspR9I CCNGG 1 cut(s) 358
Mva1269I GAATGC 2 cut(s) 150, 193
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 2 cut(s) 68, 268
NdeII GATC 1 cut(s) 337
NlaIV GGNNCC 1 cut(s) 58
PaeR7I CTCGAG 1 cut(s) 402
PctI GAATGC 2 cut(s) 150, 193
PkrI GCNGC 2 cut(s) 64, 256
Psp6I CCWGG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 1 cut(s) 58
PspXI VCTCGAGB 1 cut(s) 402
RsaI GTAC 2 cut(s) 382, 448
RsaNI GTAC 2 cut(s) 381, 447
RseI CAYNNNNRTG 2 cut(s) 144, 368
SacII CCGCGG 1 cut(s) 63
SaqAI TTAA 2 cut(s) 174, 470
SatI GCNGC 2 cut(s) 63, 255
Sau3AI GATC 1 cut(s) 337
ScrFI CCNGG 1 cut(s) 358
SduI GDGCHC 1 cut(s) 217
SetI ASST 5 cut(s) 161, 224, 320, 382, 403
Sfr274I CTCGAG 1 cut(s) 402
Sfr303I CCGCGG 1 cut(s) 63
SgrBI CCGCGG 1 cut(s) 63
SlaI CTCGAG 1 cut(s) 402
SmiMI CAYNNNNRTG 2 cut(s) 144, 368
SmlI CTYRAG 1 cut(s) 402
SmoI CTYRAG 1 cut(s) 402
Sse9I AATT 1 cut(s) 18
SsiI CCGC 2 cut(s) 60, 62
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 356
TaqI TCGA 2 cut(s) 162, 403
TasI AATT 1 cut(s) 18
TatI WGTACW 1 cut(s) 446
TauI GCSGC 1 cut(s) 65
Tru1I TTAA 2 cut(s) 174, 470
Tru9I TTAA 2 cut(s) 174, 470
TscAI CASTG 2 cut(s) 313, 375
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 2 cut(s) 197, 385
TspRI CASTG 2 cut(s) 313, 375
VneI GTGCAC 1 cut(s) 213
XhoI CTCGAG 1 cut(s) 402
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.