Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g03690 FvH4_5g03700 FvH4_5g21200 |
| malus_domestica | MD14G1116600.v1.1 |
| prunus_persica | Prupe.5G111000_v2.0.a1 Prupe.5G111100_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr7g0196031 RchiOBHm_Chr7g0196041 |
| rosa_laevigata | RLG00000004071 RLG00000004072 |
| rosa_multiflora | Rmu_sc0001640.1_g000010 Rmu_sc0001640.1_g000011 |
| rosa_roxburghii | Rroxscaffold_3G00259910 Rroxscaffold_3G00259920 |
| rosa_rugosa | Rorug07G0029100 Rorug07G0029200 |
| rosa_samantha | Rh7AG155800 Rh7AG155900 Rh7BG157600 Rh7CG161200 Rh7CG161300 Rh7DG156700 Rh7DG156800 |
| rosa_wichuraiana | Rw7G013320 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 458 |
| AccI | GTMKAC | 1 cut(s) | 121 |
| AciI | CCGC | 2 cut(s) | 203, 468 |
| AcsI | RAATTY | 1 cut(s) | 166 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 14, 209 |
| AgsI | TTSAA | 2 cut(s) | 157, 385 |
| AjnI | CCWGG | 1 cut(s) | 206 |
| AluBI | AGCT | 2 cut(s) | 29, 65 |
| AluI | AGCT | 2 cut(s) | 29, 65 |
| Alw26I | GTCTC | 1 cut(s) | 506 |
| ApeKI | GCWGC | 1 cut(s) | 185 |
| ApoI | RAATTY | 1 cut(s) | 166 |
| AsuC2I | CCSGG | 1 cut(s) | 421 |
| AsuHPI | GGTGA | 3 cut(s) | 101, 192, 542 |
| BaeGI | GKGCMC | 1 cut(s) | 319 |
| BbsI | GAAGAC | 2 cut(s) | 373, 539 |
| BbvI | GCAGC | 1 cut(s) | 197 |
| BceAI | ACGGC | 1 cut(s) | 175 |
| BciT130I | CCWGG | 1 cut(s) | 208 |
| BcnI | CCSGG | 1 cut(s) | 421 |
| BcoDI | GTCTC | 1 cut(s) | 506 |
| BfaI | CTAG | 3 cut(s) | 20, 447, 492 |
| BfmI | CTRYAG | 1 cut(s) | 183 |
| BisI | GCNGC | 1 cut(s) | 186 |
| BlsI | GCNGC | 1 cut(s) | 187 |
| Bme1390I | CCNGG | 2 cut(s) | 208, 421 |
| BmiI | GGNNCC | 1 cut(s) | 6 |
| BmrFI | CCNGG | 2 cut(s) | 208, 421 |
| BmrI | ACTGGG | 2 cut(s) | 299, 483 |
| BmsI | GCATC | 1 cut(s) | 417 |
| BmuI | ACTGGG | 2 cut(s) | 299, 483 |
| BpiI | GAAGAC | 2 cut(s) | 373, 539 |
| BpuMI | CCSGG | 1 cut(s) | 421 |
| BsaJI | CCNNGG | 4 cut(s) | 206, 207, 319, 420 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 14, 209 |
| Bse1I | ACTGG | 2 cut(s) | 305, 478 |
| BseBI | CCWGG | 1 cut(s) | 208 |
| BseDI | CCNNGG | 4 cut(s) | 206, 207, 319, 420 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 14, 209 |
| BseNI | ACTGG | 2 cut(s) | 305, 478 |
| BseRI | GAGGAG | 3 cut(s) | 38, 93, 96 |
| BseSI | GKGCMC | 1 cut(s) | 319 |
| BseXI | GCAGC | 1 cut(s) | 197 |
| BsiSI | CCGG | 1 cut(s) | 420 |
| BslFI | GGGAC | 3 cut(s) | 464, 484, 491 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 14, 209 |
| BsmAI | GTCTC | 1 cut(s) | 506 |
| BsmFI | GGGAC | 3 cut(s) | 464, 484, 491 |
| Bsp1286I | GDGCHC | 1 cut(s) | 319 |
| BspACI | CCGC | 2 cut(s) | 203, 468 |
| BspHI | TCATGA | 1 cut(s) | 486 |
| BspLI | GGNNCC | 1 cut(s) | 6 |
| BspMAI | CTGCAG | 1 cut(s) | 187 |
| BsrI | ACTGG | 2 cut(s) | 305, 478 |
| BssECI | CCNNGG | 4 cut(s) | 206, 207, 319, 420 |
| BssT1I | CCWWGG | 1 cut(s) | 319 |
| Bst2UI | CCWGG | 1 cut(s) | 208 |
| Bst6I | CTCTTC | 2 cut(s) | 38, 44 |
| BstMAI | GTCTC | 1 cut(s) | 506 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 62, 185 |
| BstNI | CCWGG | 1 cut(s) | 208 |
| BstSCI | CCNGG | 2 cut(s) | 206, 419 |
| BstSFI | CTRYAG | 1 cut(s) | 183 |
| BstSLI | GKGCMC | 1 cut(s) | 319 |
| BstV1I | GCAGC | 1 cut(s) | 197 |
| BstV2I | GAAGAC | 2 cut(s) | 373, 539 |
| BtsI | GCAGTG | 2 cut(s) | 180, 354 |
| BtsIMutI | CAGTG | 2 cut(s) | 180, 354 |
| CciI | TCATGA | 1 cut(s) | 486 |
| CseI | GACGC | 1 cut(s) | 527 |
| CviAII | CATG | 3 cut(s) | 117, 360, 487 |
| CviJI | RGCY | 8 cut(s) | 29, 65, 94, 188, 240, 324, 364, 369 |
| CviKI_1 | RGCY | 8 cut(s) | 29, 65, 94, 188, 240, 324, 364, 369 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 458 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 458 |
| Eam1104I | CTCTTC | 2 cut(s) | 38, 44 |
| EarI | CTCTTC | 2 cut(s) | 38, 44 |
| Eco130I | CCWWGG | 1 cut(s) | 319 |
| EcoRI | GAATTC | 1 cut(s) | 166 |
| EcoRII | CCWGG | 1 cut(s) | 206 |
| EcoT14I | CCWWGG | 1 cut(s) | 319 |
| ErhI | CCWWGG | 1 cut(s) | 319 |
| FaeI | CATG | 3 cut(s) | 120, 363, 490 |
| FaiI | YATR | 8 cut(s) | 62, 87, 118, 330, 336, 361, 488, 570 |
| FaqI | GGGAC | 3 cut(s) | 464, 484, 491 |
| FatI | CATG | 3 cut(s) | 116, 359, 486 |
| FauI | CCCGC | 1 cut(s) | 461 |
| FblI | GTMKAC | 1 cut(s) | 121 |
| Fnu4HI | GCNGC | 1 cut(s) | 186 |
| Fsp4HI | GCNGC | 1 cut(s) | 186 |
| FspBI | CTAG | 3 cut(s) | 20, 447, 492 |
| GluI | GCNGC | 1 cut(s) | 186 |
| HapII | CCGG | 1 cut(s) | 420 |
| HgaI | GACGC | 1 cut(s) | 527 |
| Hin1II | CATG | 3 cut(s) | 120, 363, 490 |
| HincII | GTYRAC | 1 cut(s) | 556 |
| HindII | GTYRAC | 1 cut(s) | 556 |
| HpaII | CCGG | 1 cut(s) | 420 |
| HphI | GGTGA | 3 cut(s) | 101, 192, 542 |
| Hpy166II | GTNNAC | 3 cut(s) | 122, 200, 556 |
| Hpy188III | TCNNGA | 1 cut(s) | 487 |
| Hpy8I | GTNNAC | 3 cut(s) | 122, 200, 556 |
| Hpy99I | CGWCG | 1 cut(s) | 269 |
| HpyCH4IV | ACGT | 1 cut(s) | 453 |
| HpyCH4V | TGCA | 4 cut(s) | 56, 185, 295, 359 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 62, 185 |
| HpySE526I | ACGT | 1 cut(s) | 453 |
| Hsp92II | CATG | 3 cut(s) | 120, 363, 490 |
| LmnI | GCTCC | 1 cut(s) | 405 |
| LpnPI | CCDG | 7 cut(s) | 193, 216, 220, 318, 325, 433, 459 |
| Lsp1109I | GCAGC | 1 cut(s) | 197 |
| LweI | GCATC | 1 cut(s) | 417 |
| MaeI | CTAG | 3 cut(s) | 20, 447, 492 |
| MaeII | ACGT | 1 cut(s) | 453 |
| MaeIII | GTNAC | 1 cut(s) | 248 |
| MboII | GAAGA | 8 cut(s) | 25, 31, 34, 145, 343, 373, 475, 539 |
| MhlI | GDGCHC | 1 cut(s) | 319 |
| MluCI | AATT | 3 cut(s) | 166, 273, 289 |
| MseI | TTAA | 1 cut(s) | 561 |
| MslI | CAYNNNNRTG | 1 cut(s) | 257 |
| MspI | CCGG | 1 cut(s) | 420 |
| MspR9I | CCNGG | 2 cut(s) | 208, 421 |
| MvaI | CCWGG | 1 cut(s) | 208 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 62, 185 |
| NciI | CCSGG | 1 cut(s) | 421 |
| NlaIII | CATG | 3 cut(s) | 120, 363, 490 |
| NlaIV | GGNNCC | 1 cut(s) | 6 |
| PagI | TCATGA | 1 cut(s) | 486 |
| PasI | CCCWGGG | 1 cut(s) | 207 |
| PkrI | GCNGC | 1 cut(s) | 187 |
| Psp6I | CCWGG | 1 cut(s) | 206 |
| PspGI | CCWGG | 1 cut(s) | 206 |
| PspN4I | GGNNCC | 1 cut(s) | 6 |
| PstI | CTGCAG | 1 cut(s) | 187 |
| RseI | CAYNNNNRTG | 1 cut(s) | 257 |
| SaqAI | TTAA | 1 cut(s) | 561 |
| SatI | GCNGC | 1 cut(s) | 186 |
| ScrFI | CCNGG | 2 cut(s) | 208, 421 |
| SduI | GDGCHC | 1 cut(s) | 319 |
| SetI | ASST | 5 cut(s) | 31, 67, 129, 134, 456 |
| SfaNI | GCATC | 1 cut(s) | 417 |
| SfcI | CTRYAG | 1 cut(s) | 183 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 257 |
| Sse9I | AATT | 3 cut(s) | 166, 273, 289 |
| SsiI | CCGC | 2 cut(s) | 203, 468 |
| SspMI | CTAG | 3 cut(s) | 20, 447, 492 |
| StyD4I | CCNGG | 2 cut(s) | 206, 419 |
| StyI | CCWWGG | 1 cut(s) | 319 |
| TaiI | ACGT | 1 cut(s) | 456 |
| TaqI | TCGA | 3 cut(s) | 264, 389, 550 |
| TasI | AATT | 3 cut(s) | 166, 273, 289 |
| Tru1I | TTAA | 1 cut(s) | 561 |
| Tru9I | TTAA | 1 cut(s) | 561 |
| TscAI | CASTG | 2 cut(s) | 187, 361 |
| TseI | GCWGC | 1 cut(s) | 185 |
| TspDTI | ATGAA | 2 cut(s) | 452, 475 |
| TspRI | CASTG | 2 cut(s) | 187, 361 |
| XapI | RAATTY | 1 cut(s) | 166 |
| XmiI | GTMKAC | 1 cut(s) | 121 |
| XspI | CTAG | 3 cut(s) | 20, 447, 492 |