FvH4_5g03700

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008133
Physical Location & Seq
Reverse (-)
2163368 .. 2163943
576 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03700.t1

Sequence Viewer

Length: 576 bp
ATGGAGTCAAAAAGTTTAGCTCCCATTCTCTTCCTCTTCTTCCTTCTCTTTGGAACCTTAACAGCTTCCCCTACCCTCAAATCCAGGCCTCACCCTCGCCGTCCTCTCCCTTGCAAACGCCTCGTTTTCTATTTCCACGATATCATCTACAATGGTAGAAACTCCAAGAATGCCACAGCAGCAATTGTGGGTGCACCCGCTTGGGGCAACAAGACCATATTGACAGGGAAAAATCATTTTGGGAATATGGTTGTGTTCGACGATCCAATCACTTTGGACAACAATTTGCACTCAACCCCGGTTGGTCGTGCTCAAGGCTTTTACTTGTACGACAAGAAAGATATATTCACTGCATGGCTCGGGTTCTCCTTCGTGTTCAACTCAACCCAACACAGAGGGAGCATCAACTTTGCTGGTGCTGATCCATTGATGAACAAGACCAGAGACATTTCGGTTATTGGTGGAACTGGTGACTTCTTCATGACTAGAGGGATTGCTACCTTGATGACTGATGCATTTGAGGGTGAAGTCTATTTCCGTCTCAAAGTTGACATTAAATTGTACGAGTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

192

Amino Acids

21.5

Weight (kDa)

9.42

Isoelectric Point (pI)

24.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 38 - 184 7e-38 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 198
AclWI GGATC 2 cut(s) 257, 416
AfaI GTAC 2 cut(s) 329, 563
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 1 cut(s) 379
AjnI CCWGG 1 cut(s) 83
AluBI AGCT 2 cut(s) 20, 65
AluI AGCT 2 cut(s) 20, 65
Alw21I GWGCWC 2 cut(s) 196, 313
Alw26I GTCTC 2 cut(s) 438, 545
Alw44I GTGCAC 1 cut(s) 192
AlwI GGATC 2 cut(s) 257, 416
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 1 cut(s) 86
ApaLI GTGCAC 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 179
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 3 cut(s) 83, 482, 536
AvaI CYCGRG 1 cut(s) 359
BaeGI GKGCMC 1 cut(s) 196
Bbv12I GWGCWC 2 cut(s) 196, 313
BbvI GCAGC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 84
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 1 cut(s) 85
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 438, 545
BfaI CTAG 1 cut(s) 486
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 2 cut(s) 85, 299
BmeT110I CYCGRG 1 cut(s) 359
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 85, 299
BmsI GCATC 2 cut(s) 411, 502
BpuEI CTTGAG 1 cut(s) 297
BpuMI CCSGG 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 297
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 472
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 472
BseSI GKGCMC 1 cut(s) 196
BseXI GCAGC 1 cut(s) 191
BshFI GGCC 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 196, 313
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 2 cut(s) 438, 545
BsmBI CGTCTC 1 cut(s) 545
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 2 cut(s) 196, 313
Bsp143I GATC 2 cut(s) 262, 421
BspACI CCGC 1 cut(s) 198
BspANI GGCC 1 cut(s) 88
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 2 cut(s) 257, 416
BsrI ACTGG 1 cut(s) 472
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 2 cut(s) 262, 421
Bst2UI CCWGG 1 cut(s) 85
Bst6I CTCTTC 2 cut(s) 35, 41
BstKTI GATC 2 cut(s) 265, 424
BstMAI GTCTC 2 cut(s) 438, 545
BstMBI GATC 2 cut(s) 262, 421
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 85
BstSCI CCNGG 2 cut(s) 83, 297
BstSLI GKGCMC 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 191
BsuRI GGCC 1 cut(s) 88
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 348
CciI TCATGA 1 cut(s) 480
Csp6I GTAC 2 cut(s) 328, 562
CviAII CATG 2 cut(s) 354, 481
CviJI RGCY 5 cut(s) 20, 65, 88, 318, 358
CviKI_1 RGCY 5 cut(s) 20, 65, 88, 318, 358
CviQI GTAC 2 cut(s) 328, 562
DpnI GATC 2 cut(s) 264, 423
DpnII GATC 2 cut(s) 262, 421
Eam1104I CTCTTC 2 cut(s) 35, 41
EarI CTCTTC 2 cut(s) 35, 41
Eco147I AGGCCT 1 cut(s) 88
Eco32I GATATC 1 cut(s) 142
Eco88I CYCGRG 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 83
EcoRV GATATC 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 517
Esp3I CGTCTC 1 cut(s) 545
FaeI CATG 2 cut(s) 357, 484
FaiI YATR 5 cut(s) 218, 248, 344, 355, 482
FatI CATG 2 cut(s) 353, 480
FauI CCCGC 1 cut(s) 205
Fnu4HI GCNGC 1 cut(s) 180
Fsp4HI GCNGC 1 cut(s) 180
FspBI CTAG 1 cut(s) 486
GluI GCNGC 1 cut(s) 180
HaeIII GGCC 1 cut(s) 88
HapII CCGG 1 cut(s) 299
Hin1II CATG 2 cut(s) 357, 484
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 299
HphI GGTGA 3 cut(s) 83, 482, 536
Hpy166II GTNNAC 2 cut(s) 194, 550
Hpy188III TCNNGA 1 cut(s) 481
Hpy8I GTNNAC 2 cut(s) 194, 550
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 53, 379
HpyCH4V TGCA 5 cut(s) 114, 194, 289, 353, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
Hsp92II CATG 2 cut(s) 357, 484
Kzo9I GATC 2 cut(s) 262, 421
LmnI GCTCC 2 cut(s) 25, 399
LpnPI CCDG 7 cut(s) 70, 97, 210, 312, 399, 453, 454
Lsp1109I GCAGC 1 cut(s) 191
LweI GCATC 2 cut(s) 411, 502
MaeI CTAG 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 470
MalI GATC 2 cut(s) 264, 423
MboI GATC 2 cut(s) 262, 421
MboII GAAGA 4 cut(s) 22, 28, 31, 469
MfeI CAATTG 1 cut(s) 183
MhlI GDGCHC 2 cut(s) 196, 313
MluCI AATT 3 cut(s) 183, 283, 557
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 9 cut(s) 44, 86, 99, 105, 114, 131, 389, 482, 514
Mph1103I ATGCAT 1 cut(s) 517
MseI TTAA 2 cut(s) 59, 555
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 85, 299
MunI CAATTG 1 cut(s) 183
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 1 cut(s) 85
MwoI GCNNNNNNNGC 1 cut(s) 179
NciI CCSGG 1 cut(s) 299
NdeII GATC 2 cut(s) 262, 421
NlaIII CATG 2 cut(s) 357, 484
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 470
NsiI ATGCAT 1 cut(s) 517
PagI TCATGA 1 cut(s) 480
PceI AGGCCT 1 cut(s) 88
PctI GAATGC 1 cut(s) 175
PkrI GCNGC 1 cut(s) 181
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 55
RsaI GTAC 2 cut(s) 329, 563
RsaNI GTAC 2 cut(s) 328, 562
SaqAI TTAA 2 cut(s) 59, 555
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 2 cut(s) 262, 421
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 85, 299
SduI GDGCHC 2 cut(s) 196, 313
SetI ASST 4 cut(s) 22, 59, 67, 503
SfaNI GCATC 2 cut(s) 411, 502
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
Sse9I AATT 3 cut(s) 183, 283, 557
SseBI AGGCCT 1 cut(s) 88
SsiI CCGC 1 cut(s) 198
SspMI CTAG 1 cut(s) 486
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 2 cut(s) 83, 297
TaqI TCGA 1 cut(s) 258
TasI AATT 3 cut(s) 183, 283, 557
Tru1I TTAA 2 cut(s) 59, 555
Tru9I TTAA 2 cut(s) 59, 555
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 2 cut(s) 446, 469
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 1 cut(s) 355
VneI GTGCAC 1 cut(s) 192
XspI CTAG 1 cut(s) 486
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.