MD14G1116600.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008133
Physical Location & Seq
Reverse (-)
18925769 .. 18926350
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1116600.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGAACAAAAAACGAGCCTAGTTTCATCTCTCTTCCTCTTCTTCCTTCTCTATGGAACCTTGGCTTCCCCGACCCTAAAACCCAGTTTTAAGCCTCGTCCTCCTCCTCACCACCCTTGCAGACACCTAGTTTTCTACTTCCACGACATTATCTACAACGGAAAAAATTCTAAAAACGCCACGGCAACCATTGTAGGTTCACCGGCTTGGGGCAACAAGACCATTTTGGCAGGACAAAACCATTTTGGTGACCTAGTTGTGTTTGATGACCCAATAACTCTGGACAACAATTTGCACTCAACCCCAGTTGGTCGTGCCCAAGGCTTCTACATTTACGACAAGAAGGAAATCTTCACTGCATGGCTTGGCTTTTCCTTTGTGTTCAACTCCACCCAACACAAAGGTAGCATCAACTTTGCTGGGGCTGACCCATTGATGAACAAGACTAGGGACATATCGGTCATTGGCGGGACGGGTGATTTCTTCATGGCTAGAGGGATTGCTACTTTGATGACTGATGCATTTGAGGGTGAAGTTTACTTTCGTCTCCGAGTTGACATTAAGTTGTATGAGTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

194

Amino Acids

21.69

Weight (kDa)

8.51

Isoelectric Point (pI)

24.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 40 - 185 2.1e-39 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 458
AciI CCGC 1 cut(s) 468
AcsI RAATTY 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 1 cut(s) 385
Alw26I GTCTC 1 cut(s) 551
ApoI RAATTY 1 cut(s) 166
Asp700I GAANNNNTTC 1 cut(s) 166
AsuHPI GGTGA 5 cut(s) 101, 192, 260, 488, 542
BaeGI GKGCMC 1 cut(s) 319
BceAI ACGGC 1 cut(s) 198
BcoDI GTCTC 1 cut(s) 551
BfaI CTAG 5 cut(s) 20, 128, 254, 447, 492
BmiI GGNNCC 1 cut(s) 58
BmrI ACTGGG 2 cut(s) 78, 299
BmsI GCATC 2 cut(s) 417, 508
BmuI ACTGGG 2 cut(s) 78, 299
BsaJI CCNNGG 3 cut(s) 60, 180, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 84, 305
BseDI CCNNGG 3 cut(s) 60, 180, 319
BseLI CCNNNNNNNGG 1 cut(s) 209
BseNI ACTGG 2 cut(s) 84, 305
BseRI GAGGAG 2 cut(s) 93, 96
BseSI GKGCMC 1 cut(s) 319
BseYI CCCAGC 1 cut(s) 419
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 2 cut(s) 464, 484
BslI CCNNNNNNNGG 1 cut(s) 209
BsmAI GTCTC 1 cut(s) 551
BsmBI CGTCTC 1 cut(s) 551
BsmFI GGGAC 2 cut(s) 464, 484
Bsp1286I GDGCHC 1 cut(s) 319
BspACI CCGC 1 cut(s) 468
BspLI GGNNCC 1 cut(s) 58
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 84, 305
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 3 cut(s) 60, 180, 319
BssT1I CCWWGG 2 cut(s) 60, 319
Bst6I CTCTTC 2 cut(s) 38, 44
BstDSI CCRYGG 1 cut(s) 180
BstEII GGTNACC 1 cut(s) 248
BstMAI GTCTC 1 cut(s) 551
BstPI GGTNACC 1 cut(s) 248
BstSLI GKGCMC 1 cut(s) 319
BtgI CCRYGG 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 354
Cfr10I RCCGGY 1 cut(s) 202
CviAII CATG 2 cut(s) 360, 487
CviJI RGCY 9 cut(s) 18, 65, 94, 206, 324, 364, 369, 425, 491
CviKI_1 RGCY 9 cut(s) 18, 65, 94, 206, 324, 364, 369, 425, 491
DrdI GACNNNNNNGTC 1 cut(s) 458
DseDI GACNNNNNNGTC 1 cut(s) 458
Eam1104I CTCTTC 2 cut(s) 38, 44
EarI CTCTTC 2 cut(s) 38, 44
Eco130I CCWWGG 2 cut(s) 60, 319
Eco91I GGTNACC 1 cut(s) 248
EcoO65I GGTNACC 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 60, 319
EcoT22I ATGCAT 1 cut(s) 523
ErhI CCWWGG 2 cut(s) 60, 319
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 2 cut(s) 363, 490
FaiI YATR 5 cut(s) 54, 361, 455, 488, 570
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 2 cut(s) 464, 484
FatI CATG 2 cut(s) 359, 486
FauI CCCGC 1 cut(s) 461
FspBI CTAG 5 cut(s) 20, 128, 254, 447, 492
GsaI CCCAGC 1 cut(s) 423
HapII CCGG 1 cut(s) 203
Hin1II CATG 2 cut(s) 363, 490
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HpaII CCGG 1 cut(s) 203
HphI GGTGA 5 cut(s) 101, 192, 260, 488, 542
Hpy166II GTNNAC 3 cut(s) 200, 538, 556
Hpy188I TCNGA 1 cut(s) 551
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 3 cut(s) 200, 538, 556
HpyAV CCTTC 2 cut(s) 56, 337
HpyCH4V TGCA 4 cut(s) 120, 295, 359, 521
Hsp92II CATG 2 cut(s) 363, 490
LpnPI CCDG 6 cut(s) 97, 216, 216, 266, 318, 405
LweI GCATC 2 cut(s) 417, 508
MaeI CTAG 5 cut(s) 20, 128, 254, 447, 492
MaeIII GTNAC 1 cut(s) 248
MboII GAAGA 5 cut(s) 25, 31, 34, 343, 475
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 2 cut(s) 166, 289
MnlI CCTC 7 cut(s) 47, 105, 111, 114, 117, 488, 520
Mph1103I ATGCAT 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 166
MseI TTAA 2 cut(s) 90, 561
MslI CAYNNNNRTG 1 cut(s) 246
MspI CCGG 1 cut(s) 203
NlaIII CATG 2 cut(s) 363, 490
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 523
PdmI GAANNNNTTC 1 cut(s) 166
PspEI GGTNACC 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 419
PspN4I GGNNCC 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 246
SaqAI TTAA 2 cut(s) 90, 561
SduI GDGCHC 1 cut(s) 319
SetI ASST 5 cut(s) 62, 129, 199, 255, 406
SfaNI GCATC 2 cut(s) 417, 508
SmiMI CAYNNNNRTG 1 cut(s) 246
Sse9I AATT 2 cut(s) 166, 289
SsiI CCGC 1 cut(s) 468
SspMI CTAG 5 cut(s) 20, 128, 254, 447, 492
StyI CCWWGG 2 cut(s) 60, 319
TaqII GACCGA 1 cut(s) 448
TasI AATT 2 cut(s) 166, 289
Tru1I TTAA 2 cut(s) 90, 561
Tru9I TTAA 2 cut(s) 90, 561
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 1 cut(s) 248
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 3 cut(s) 15, 452, 475
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 1 cut(s) 361
XapI RAATTY 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 166
XspI CTAG 5 cut(s) 20, 128, 254, 447, 492
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.