FvH4_5g21200

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008133
Physical Location & Seq
Forward (+)
12725011 .. 12725800
790 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21200.t1

Sequence Viewer

Length: 570 bp
ATGGAGTCAAAATCTCTAGCTTCAACTCTCTTCCTCTTCTTCCTCCTCTTTGGAACCTCAGCAGCTTCCCCCACCCTCAAATCCAAGCCTCATCATCCTCTTCCTTGCAAACGCCTCGTTTTCTATCTCCACGATATTGTCTACAACGGCAAAAATTCAAAGAATGCAACATCAGCCATCGTGGGTGCACCCGCTTGGGGCAACAAGACCATACTGGCAAAGGACAACCATTTCGGGGATGTGGTTGTGTTCGACGATCCGATCACTTCAGACAACAACTTGCACTCGACCCCGGTTGGTCGCGCGCAAGGCTTTTACTTGTACGACAGGAAAGTTGCATTCACTGCTTTTTTCGGGATATCCTTCGTGTTCAACTCAACCCAACACAAAGGAAGCATCAACTGTGTTGGGGCTGATCCAACGGCGGCTAAGACTAGAGACATTTCAGTTGTTGGTGGAACTGGTGACTTCTTCATGGCTAGAGGGATTGCTACTTTGATGGGTGATGATGTTGAGGGTGATGTCTATTTCCGTCTCAAAGTTGACGTGAAGTTGTACGAGTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

190

Amino Acids

20.68

Weight (kDa)

8.4

Isoelectric Point (pI)

15.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 36 - 183 4.8e-37 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 141
AccII CGCG 2 cut(s) 303, 305
AciI CCGC 2 cut(s) 192, 425
AclWI GGATC 2 cut(s) 251, 410
AcsI RAATTY 1 cut(s) 154
AcuI CTGAAG 1 cut(s) 252
AfaI GTAC 2 cut(s) 323, 557
AfiI CCNNNNNNNGG 2 cut(s) 197, 235
AgsI TTSAA 3 cut(s) 24, 159, 373
AjiI CACGTC 1 cut(s) 547
AluBI AGCT 2 cut(s) 20, 65
AluI AGCT 2 cut(s) 20, 65
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 2 cut(s) 432, 539
Alw44I GTGCAC 1 cut(s) 186
AlwI GGATC 2 cut(s) 251, 410
ApaLI GTGCAC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 1 cut(s) 154
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AspLEI GCGC 2 cut(s) 305, 307
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 476, 515, 530
BaeGI GKGCMC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 190
BbvCI CCTCAGC 1 cut(s) 58
BbvI GCAGC 1 cut(s) 74
BccI CCATC 2 cut(s) 185, 493
BceAI ACGGC 2 cut(s) 163, 438
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 432, 539
BfaI CTAG 3 cut(s) 17, 435, 480
BisI GCNGC 2 cut(s) 63, 426
BlsI GCNGC 2 cut(s) 64, 427
Bme1390I CCNGG 1 cut(s) 293
BmgBI CACGTC 1 cut(s) 547
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 405
Bpu10I CCTNAGC 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 291
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 235
Bse1I ACTGG 2 cut(s) 219, 466
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 2 cut(s) 94, 244
BseLI CCNNNNNNNGG 2 cut(s) 197, 235
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 219, 466
BsePI GCGCGC 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 190
BseXI GCAGC 1 cut(s) 74
Bsh1236I CGCG 2 cut(s) 303, 305
BsiHKAI GWGCWC 1 cut(s) 190
BsiSI CCGG 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 197, 235
BsmAI GTCTC 2 cut(s) 432, 539
BsmBI CGTCTC 1 cut(s) 539
BsmI GAATGC 2 cut(s) 169, 338
Bsp1286I GDGCHC 1 cut(s) 190
Bsp143I GATC 3 cut(s) 256, 261, 415
BspACI CCGC 2 cut(s) 192, 425
BspCNI CTCAG 1 cut(s) 71
BspFNI CGCG 2 cut(s) 303, 305
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 2 cut(s) 251, 410
BsrI ACTGG 2 cut(s) 219, 466
BssECI CCNNGG 1 cut(s) 291
BssHII GCGCGC 1 cut(s) 303
BssMI GATC 3 cut(s) 256, 261, 415
Bst4CI ACNGT 1 cut(s) 404
Bst6I CTCTTC 3 cut(s) 35, 41, 105
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 2 cut(s) 58, 429
BstF5I GGATG 2 cut(s) 94, 244
BstFNI CGCG 2 cut(s) 303, 305
BstHHI GCGC 2 cut(s) 305, 307
BstKTI GATC 3 cut(s) 259, 264, 418
BstMAI GTCTC 2 cut(s) 432, 539
BstMBI GATC 3 cut(s) 256, 261, 415
BstMWI GCNNNNNNNGC 3 cut(s) 173, 309, 344
BstSCI CCNGG 1 cut(s) 291
BstSLI GKGCMC 1 cut(s) 190
BstUI CGCG 2 cut(s) 303, 305
BstV1I GCAGC 1 cut(s) 74
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 2 cut(s) 94, 244
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 305
CfoI GCGC 2 cut(s) 305, 307
Csp6I GTAC 2 cut(s) 322, 556
CviAII CATG 1 cut(s) 475
CviJI RGCY 8 cut(s) 20, 65, 88, 176, 312, 413, 428, 479
CviKI_1 RGCY 8 cut(s) 20, 65, 88, 176, 312, 413, 428, 479
CviQI GTAC 2 cut(s) 322, 556
DdeI CTNAG 2 cut(s) 58, 429
DpnI GATC 3 cut(s) 258, 263, 417
DpnII GATC 3 cut(s) 256, 261, 415
Eam1104I CTCTTC 3 cut(s) 35, 41, 105
EarI CTCTTC 3 cut(s) 35, 41, 105
Eco32I GATATC 1 cut(s) 360
Eco57I CTGAAG 1 cut(s) 252
EcoRV GATATC 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 1 cut(s) 478
FaiI YATR 2 cut(s) 212, 476
FatI CATG 1 cut(s) 474
FauI CCCGC 1 cut(s) 199
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 2 cut(s) 63, 426
FokI GGATG 2 cut(s) 81, 251
Fsp4HI GCNGC 2 cut(s) 63, 426
FspBI CTAG 3 cut(s) 17, 435, 480
GlaI GCGC 2 cut(s) 304, 306
GluI GCNGC 2 cut(s) 63, 426
HapII CCGG 1 cut(s) 293
HhaI GCGC 2 cut(s) 305, 307
Hin1II CATG 1 cut(s) 478
Hin6I GCGC 2 cut(s) 303, 305
HinP1I GCGC 2 cut(s) 303, 305
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 293
HphI GGTGA 3 cut(s) 476, 515, 530
Hpy166II GTNNAC 3 cut(s) 142, 188, 544
Hpy188I TCNGA 2 cut(s) 261, 271
Hpy188III TCNNGA 1 cut(s) 355
Hpy8I GTNNAC 3 cut(s) 142, 188, 544
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 5 cut(s) 108, 167, 188, 283, 338
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 309, 344
HpyF3I CTNAG 2 cut(s) 58, 429
HpySE526I ACGT 1 cut(s) 546
Hsp92II CATG 1 cut(s) 478
HspAI GCGC 2 cut(s) 303, 305
Kzo9I GATC 3 cut(s) 256, 261, 415
LpnPI CCDG 4 cut(s) 200, 306, 313, 447
Lsp1109I GCAGC 1 cut(s) 74
LweI GCATC 1 cut(s) 405
MaeI CTAG 3 cut(s) 17, 435, 480
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 1 cut(s) 464
MalI GATC 3 cut(s) 258, 263, 417
MboI GATC 3 cut(s) 256, 261, 415
MboII GAAGA 5 cut(s) 22, 28, 31, 92, 463
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 1 cut(s) 154
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 443
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 293
Mva1269I GAATGC 2 cut(s) 169, 338
MvnI CGCG 2 cut(s) 303, 305
MwoI GCNNNNNNNGC 3 cut(s) 173, 309, 344
NciI CCSGG 1 cut(s) 293
NdeII GATC 3 cut(s) 256, 261, 415
NlaIII CATG 1 cut(s) 478
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 464
PauI GCGCGC 1 cut(s) 303
PctI GAATGC 2 cut(s) 169, 338
PkrI GCNGC 2 cut(s) 64, 427
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 55
PteI GCGCGC 1 cut(s) 303
RsaI GTAC 2 cut(s) 323, 557
RsaNI GTAC 2 cut(s) 322, 556
SatI GCNGC 2 cut(s) 63, 426
Sau3AI GATC 3 cut(s) 256, 261, 415
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 1 cut(s) 190
SetI ASST 4 cut(s) 22, 59, 67, 549
SfaNI GCATC 1 cut(s) 405
Sse9I AATT 1 cut(s) 154
SsiI CCGC 2 cut(s) 192, 425
SspMI CTAG 3 cut(s) 17, 435, 480
StyD4I CCNGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 404
TaiI ACGT 1 cut(s) 549
TaqI TCGA 2 cut(s) 252, 287
TasI AATT 1 cut(s) 154
TauI GCSGC 1 cut(s) 428
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 1 cut(s) 463
TspGWI ACGGA 1 cut(s) 521
TspRI CASTG 1 cut(s) 349
VneI GTGCAC 1 cut(s) 186
XapI RAATTY 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 141
XspI CTAG 3 cut(s) 17, 435, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.