Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g03690 FvH4_5g03700 FvH4_5g21200 |
| malus_domestica | MD14G1116600.v1.1 |
| prunus_persica | Prupe.5G111000_v2.0.a1 Prupe.5G111100_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr7g0196031 RchiOBHm_Chr7g0196041 |
| rosa_laevigata | RLG00000004071 RLG00000004072 |
| rosa_multiflora | Rmu_sc0001640.1_g000010 Rmu_sc0001640.1_g000011 |
| rosa_roxburghii | Rroxscaffold_3G00259910 Rroxscaffold_3G00259920 |
| rosa_rugosa | Rorug07G0029100 Rorug07G0029200 |
| rosa_samantha | Rh7AG155800 Rh7AG155900 Rh7BG157600 Rh7CG161200 Rh7CG161300 Rh7DG156700 Rh7DG156800 |
| rosa_wichuraiana | Rw7G013320 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 250 |
| AccII | CGCG | 1 cut(s) | 486 |
| AclWI | GGATC | 2 cut(s) | 197, 356 |
| AfaI | GTAC | 4 cut(s) | 269, 406, 473, 503 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 143 |
| AgsI | TTSAA | 1 cut(s) | 460 |
| AjnI | CCWGG | 1 cut(s) | 380 |
| AluBI | AGCT | 3 cut(s) | 61, 140, 174 |
| AluI | AGCT | 3 cut(s) | 61, 140, 174 |
| Alw21I | GWGCWC | 1 cut(s) | 136 |
| AlwI | GGATC | 2 cut(s) | 197, 356 |
| Ama87I | CYCGRG | 1 cut(s) | 426 |
| AspLEI | GCGC | 2 cut(s) | 251, 488 |
| AsuC2I | CCSGG | 2 cut(s) | 24, 38 |
| AvaI | CYCGRG | 1 cut(s) | 426 |
| BanII | GRGCYC | 1 cut(s) | 43 |
| Bbv12I | GWGCWC | 1 cut(s) | 136 |
| BciT130I | CCWGG | 1 cut(s) | 382 |
| BcnI | CCSGG | 2 cut(s) | 24, 38 |
| Bme1390I | CCNGG | 3 cut(s) | 24, 38, 382 |
| BmeT110I | CYCGRG | 1 cut(s) | 426 |
| BmrFI | CCNGG | 3 cut(s) | 24, 38, 382 |
| BmrI | ACTGGG | 1 cut(s) | 233 |
| BmsI | GCATC | 2 cut(s) | 351, 442 |
| BmuI | ACTGGG | 1 cut(s) | 233 |
| BpuMI | CCSGG | 2 cut(s) | 24, 38 |
| BsaJI | CCNNGG | 1 cut(s) | 381 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 143 |
| Bse118I | RCCGGY | 1 cut(s) | 407 |
| Bse1I | ACTGG | 1 cut(s) | 239 |
| BseBI | CCWGG | 1 cut(s) | 382 |
| BseDI | CCNNGG | 1 cut(s) | 381 |
| BseGI | GGATG | 1 cut(s) | 323 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 143 |
| BseMII | CTCAG | 1 cut(s) | 480 |
| BseNI | ACTGG | 1 cut(s) | 239 |
| Bsh1236I | CGCG | 1 cut(s) | 486 |
| BsiHKAI | GWGCWC | 1 cut(s) | 136 |
| BsiHKCI | CYCGRG | 1 cut(s) | 426 |
| BsiSI | CCGG | 4 cut(s) | 24, 37, 408, 478 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 143 |
| BsmI | GAATGC | 3 cut(s) | 106, 115, 455 |
| BsoBI | CYCGRG | 1 cut(s) | 426 |
| Bsp1286I | GDGCHC | 2 cut(s) | 43, 136 |
| Bsp143I | GATC | 2 cut(s) | 202, 361 |
| BspCNI | CTCAG | 1 cut(s) | 481 |
| BspFNI | CGCG | 1 cut(s) | 486 |
| BspPI | GGATC | 2 cut(s) | 197, 356 |
| BsrFI | RCCGGY | 1 cut(s) | 407 |
| BsrI | ACTGG | 1 cut(s) | 239 |
| BssAI | RCCGGY | 1 cut(s) | 407 |
| BssECI | CCNNGG | 1 cut(s) | 381 |
| BssMI | GATC | 2 cut(s) | 202, 361 |
| Bst2UI | CCWGG | 1 cut(s) | 382 |
| Bst4CI | ACNGT | 1 cut(s) | 51 |
| BstDEI | CTNAG | 2 cut(s) | 214, 489 |
| BstF5I | GGATG | 1 cut(s) | 323 |
| BstFNI | CGCG | 1 cut(s) | 486 |
| BstHHI | GCGC | 2 cut(s) | 251, 488 |
| BstKTI | GATC | 2 cut(s) | 205, 364 |
| BstMBI | GATC | 2 cut(s) | 202, 361 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 110, 119, 255, 408 |
| BstNI | CCWGG | 1 cut(s) | 382 |
| BstSCI | CCNGG | 3 cut(s) | 22, 36, 380 |
| BstUI | CGCG | 1 cut(s) | 486 |
| BtsCI | GGATG | 1 cut(s) | 323 |
| BtsI | GCAGTG | 1 cut(s) | 288 |
| BtsIMutI | CAGTG | 3 cut(s) | 288, 365, 399 |
| CfoI | GCGC | 2 cut(s) | 251, 488 |
| Cfr10I | RCCGGY | 1 cut(s) | 407 |
| Csp6I | GTAC | 4 cut(s) | 268, 405, 472, 502 |
| CviAII | CATG | 2 cut(s) | 187, 421 |
| CviQI | GTAC | 4 cut(s) | 268, 405, 472, 502 |
| DdeI | CTNAG | 2 cut(s) | 214, 489 |
| DpnI | GATC | 2 cut(s) | 204, 363 |
| DpnII | GATC | 2 cut(s) | 202, 361 |
| Eco24I | GRGCYC | 1 cut(s) | 43 |
| Eco88I | CYCGRG | 1 cut(s) | 426 |
| EcoRII | CCWGG | 1 cut(s) | 380 |
| EcoT22I | ATGCAT | 1 cut(s) | 457 |
| EcoT38I | GRGCYC | 1 cut(s) | 43 |
| FaeI | CATG | 2 cut(s) | 190, 424 |
| FaiI | YATR | 4 cut(s) | 158, 188, 284, 422 |
| FatI | CATG | 2 cut(s) | 186, 420 |
| FokI | GGATG | 1 cut(s) | 310 |
| FriOI | GRGCYC | 1 cut(s) | 43 |
| FspI | TGCGCA | 1 cut(s) | 250 |
| GlaI | GCGC | 2 cut(s) | 250, 487 |
| HapII | CCGG | 4 cut(s) | 24, 37, 408, 478 |
| HhaI | GCGC | 2 cut(s) | 251, 488 |
| Hin1II | CATG | 2 cut(s) | 190, 424 |
| Hin6I | GCGC | 2 cut(s) | 249, 486 |
| HinP1I | GCGC | 2 cut(s) | 249, 486 |
| HinfI | GANTC | 1 cut(s) | 33 |
| HpaII | CCGG | 4 cut(s) | 24, 37, 408, 478 |
| Hpy166II | GTNNAC | 1 cut(s) | 472 |
| Hpy188III | TCNNGA | 1 cut(s) | 181 |
| Hpy8I | GTNNAC | 1 cut(s) | 472 |
| HpyCH4III | ACNGT | 1 cut(s) | 51 |
| HpyCH4V | TGCA | 2 cut(s) | 113, 455 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 110, 119, 255, 408 |
| HpyF3I | CTNAG | 2 cut(s) | 214, 489 |
| Hsp92II | CATG | 2 cut(s) | 190, 424 |
| HspAI | GCGC | 2 cut(s) | 249, 486 |
| Kzo9I | GATC | 2 cut(s) | 202, 361 |
| LmnI | GCTCC | 1 cut(s) | 131 |
| LweI | GCATC | 2 cut(s) | 351, 442 |
| MaeIII | GTNAC | 1 cut(s) | 13 |
| MalI | GATC | 2 cut(s) | 204, 363 |
| MboI | GATC | 2 cut(s) | 202, 361 |
| MboII | GAAGA | 1 cut(s) | 409 |
| MhlI | GDGCHC | 2 cut(s) | 43, 136 |
| MlyI | GAGTC | 1 cut(s) | 42 |
| MmeI | TCCRAC | 1 cut(s) | 109 |
| MnlI | CCTC | 1 cut(s) | 422 |
| Mph1103I | ATGCAT | 1 cut(s) | 457 |
| MseI | TTAA | 1 cut(s) | 318 |
| MslI | CAYNNNNRTG | 2 cut(s) | 191, 443 |
| MspI | CCGG | 4 cut(s) | 24, 37, 408, 478 |
| MspR9I | CCNGG | 3 cut(s) | 24, 38, 382 |
| Mva1269I | GAATGC | 3 cut(s) | 106, 115, 455 |
| MvaI | CCWGG | 1 cut(s) | 382 |
| MvnI | CGCG | 1 cut(s) | 486 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 110, 119, 255, 408 |
| NciI | CCSGG | 2 cut(s) | 24, 38 |
| NdeII | GATC | 2 cut(s) | 202, 361 |
| NlaIII | CATG | 2 cut(s) | 190, 424 |
| NsbI | TGCGCA | 1 cut(s) | 250 |
| NsiI | ATGCAT | 1 cut(s) | 457 |
| PaeR7I | CTCGAG | 1 cut(s) | 426 |
| PctI | GAATGC | 3 cut(s) | 106, 115, 455 |
| PleI | GAGTC | 1 cut(s) | 41 |
| PpsI | GAGTC | 1 cut(s) | 41 |
| Psp6I | CCWGG | 1 cut(s) | 380 |
| PspGI | CCWGG | 1 cut(s) | 380 |
| PspXI | VCTCGAGB | 1 cut(s) | 426 |
| RsaI | GTAC | 4 cut(s) | 269, 406, 473, 503 |
| RsaNI | GTAC | 4 cut(s) | 268, 405, 472, 502 |
| RseI | CAYNNNNRTG | 2 cut(s) | 191, 443 |
| SaqAI | TTAA | 1 cut(s) | 318 |
| Sau3AI | GATC | 2 cut(s) | 202, 361 |
| SchI | GAGTC | 1 cut(s) | 42 |
| ScrFI | CCNGG | 3 cut(s) | 24, 38, 382 |
| SduI | GDGCHC | 2 cut(s) | 43, 136 |
| SetI | ASST | 4 cut(s) | 63, 142, 176, 215 |
| SfaNI | GCATC | 2 cut(s) | 351, 442 |
| Sfr274I | CTCGAG | 1 cut(s) | 426 |
| SlaI | CTCGAG | 1 cut(s) | 426 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 191, 443 |
| SmlI | CTYRAG | 1 cut(s) | 426 |
| SmoI | CTYRAG | 1 cut(s) | 426 |
| StyD4I | CCNGG | 3 cut(s) | 22, 36, 380 |
| TaaI | ACNGT | 1 cut(s) | 51 |
| TaqI | TCGA | 1 cut(s) | 427 |
| TatI | WGTACW | 1 cut(s) | 471 |
| Tru1I | TTAA | 1 cut(s) | 318 |
| Tru9I | TTAA | 1 cut(s) | 318 |
| TscAI | CASTG | 3 cut(s) | 295, 372, 399 |
| TspDTI | ATGAA | 2 cut(s) | 386, 409 |
| TspGWI | ACGGA | 1 cut(s) | 108 |
| TspRI | CASTG | 3 cut(s) | 295, 372, 399 |
| XhoI | CTCGAG | 1 cut(s) | 426 |
| Zsp2I | ATGCAT | 1 cut(s) | 457 |