Rorug07G0029200

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008133
Physical Location & Seq
Forward (+)
2030823 .. 2033527
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0029200.1

Sequence Viewer

Length: 2238 bp
ATGGTCATGGAATTTGCCACTAGCGTTGCAGAGAGCGTCTTGGGTAGGCTAGCCTCTTATGCTGCCCAAGAAATCTGCTTGGCATGGGGTGCCCAACTTGAGTTGCAAAAGCTCAAGGAGACCTTGTTAGACATTAAAGACGTCCTCCAGGATGCGGAGGAAAAGCAAGTGAAGAATCCTATAATCAGCCGTTGGTTACGAGACGTCAAAGATGTTTGCAACGATGCTGAGGATGTACTGGACGAATATGAGTTCCGAAAGCTCCGACTCAAAGTGCTCATCAATGACCATTGGACAGTCAAAGGAGAGGTACACCAATTCTTCACCCATTGGAATCCTGTTGTGTTTAACTTCAAAATGGGTCATAAATTAAAACACATTAGGGAACGACTAGTTCAAATTGATCATGATGGAAAGACAAAGTTTTCTCTTGTTTTAAAAGTTGTTCAACAAACTGAAGTTTTTCCAGCACCCTTGAGGCCAAATGGTAATAAAAAGGAGACAGACTCTTTACTGGAAGGTCATGTTGTTGGGAGAGACGGTGATAAGGAAAAAATTTTCCGTTTCTTTGATGACACCCTTTCCTCCAATGTCGATAATGTTTCAGTTATTTCCATCTTTGGGTTAGCAGGGTTGGGAAAAACTACACTTGCTAAATCAGTGTATAATGATACTATGGTAGAGAACAAATTTGATATGAGAATGTGGTTTTTGTTAGTGTTGGACGATGTTTGGGATACAGAGTCGATAGGAGTTACAAATCAGAAGTGGAGAGAGTTGAAAACTTTGTTAGATGTTGGAGCCAGAGGAAGTAAAATCATCGTAACAACACGTAATGAATCAGTAGCTTCACTTGTGCACCCTATACATAAACATCAGTTAGAAGGTCTTTCCCAGGAGGATTCCATGACTTTGTTTAAAAAAAATGCATTTAACGAAGATGAGGAGTCACACTATCAACACTTGATGAAAATTGGAAAGGGCATTGTGGAGAAGTGTGGAGGGGTTCCCTTAGCACTAGCTACTCTAGGTGGTCTGCTCAAATTGAAAAGGGAGCAACGACATCCTTGTTTTGCCTTTTGTTCACTTTACCCCAATGATTATGAATTTATCAGTGATGAAATAGTCCCATTATGGATGGCACAAGGCTTCCTCAAGTCCTCAAGACAAAATGAAGATTTTGGCGAGATGGGCTTAGACTATATAAGACAGTTTTGTTCCAGGTCTTTGTTTCAACTTGATATTGATTTGAAGACTAATGTGAAGTTCAAAATTCATGATTTAGTGCACAACTTAGCAACATCAGTGGCAGGGGTAGAGTACTCCACAATTAATTTTCGTCCCTCTAATGCTTTTGAAAAAGTCCGGCACGTGTCAATATCAAAAAAAGACTTGTCTGGGGAAGAGGCAGGAGTCCCGACATTCATTCTCCGGTCGGAGAAACTACGGACCATTCTAAATCTAGATACTGATGTTGGGATCTCCAATCAATATTTTCTAAAGACATGCATCTTGAGATTCAAGTATCTACGTGCGCTAGATGTGTGTCGTTCTACTCTTGAGGGGCTACCAAGTTCCATTGGTAACTTGATTCATTTAAGATATCTCAAGCTGGATTCTAATAAGAAGATAAAACAGCTTCCTAGTTCCATTTGCAAGCTCTTGAATTTGCAGTTCTTAAGCCTTGACCGTTGTGATGCACTTGAGGAGTTACCCAAAGGCATAGGGAACCTGATCAACCTTAGATTTTTGGATATAACTACACAGCAGATGTATTTTCCGAAAGGAGTATTTAGACGCCTCACTGTACTTCAATCCTTATTGATTGGGAGTTGTGCGAATCTGAAATCTCTGGGCGAAGAGATCGAGTACCTCACTAACCTTCGTCAACTGGAGATTTTGAACTGTGGAAATCTGGAGTCCTTGCCAGAACTTGACTGCGTTACATACTTTGGTGATCGCCAATTACACTTTACAGAGCATGATAATCAGAGGATTTGGTTGCAAAACCTCACATCGCTGGAGCAACTGAGGATTTGGAATTGCCCCAAATTGTCTGCTTTGCCGCAAGGGATGCATTGCCTCACTGCCTTGAGAGAATTGAGCATTGCTGGGTGTCCTGAATTGAGAAAAAGATGCCAAAGGGAAACAGGAGAGGATTGGTCCAAGATCAAGCATGTCCCAGAAATTATATTTGATGACTCTGAGGATGAAGGTGCCGATGATATCGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

745

Amino Acids

85.26

Weight (kDa)

6.07

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 95 8.6e-23 Rx N-terminal domain
NB-ARC PF00931 193 - 236 8.5e-09 NB-ARC domain
NB-ARC PF00931 237 - 314 1.1e-12 NB-ARC domain
WHD_DRP PF23559 363 - 433 2.9e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 504 - 703 2.1e-23 Leucine-rich repeat region
LRR_13 PF23286 517 - 644 2.5e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_RPS2 PF23247 585 - 710 1.2e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 144, 207
AccB1I GGYRCC 2 cut(s) 89, 2216
AciI CCGC 2 cut(s) 155, 2066
AclWI GGATC 1 cut(s) 1487
AcsI RAATTY 6 cut(s) 11, 555, 689, 1106, 1272, 1666
AcuI CTGAAG 1 cut(s) 477
AcvI CACGTG 1 cut(s) 1372
AcyI GRCGYC 3 cut(s) 141, 204, 1798
AfaI GTAC 5 cut(s) 237, 312, 1322, 1809, 1871
AfiI CCNNNNNNNGG 2 cut(s) 154, 621
AflII CTTAAG 1 cut(s) 1678
AflIII ACRYGT 2 cut(s) 830, 1371
AhlI ACTAGT 1 cut(s) 391
AjnI CCWGG 3 cut(s) 147, 894, 1220
AluBI AGCT 7 cut(s) 112, 262, 848, 1022, 1612, 1639, 1660
AluI AGCT 7 cut(s) 112, 262, 848, 1022, 1612, 1639, 1660
Alw21I GWGCWC 3 cut(s) 279, 861, 1290
Alw26I GTCTC 4 cut(s) 113, 195, 494, 531
Alw44I GTGCAC 2 cut(s) 857, 1286
AlwI GGATC 1 cut(s) 1487
AoxI GGCC 1 cut(s) 479
ApaLI GTGCAC 2 cut(s) 857, 1286
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 6 cut(s) 11, 555, 689, 1106, 1272, 1666
AseI ATTAAT 1 cut(s) 1332
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 1 cut(s) 1537
AspS9I GGNCC 2 cut(s) 1449, 2163
AsuHPI GGTGA 3 cut(s) 316, 554, 1967
AsuNHI GCTAGC 1 cut(s) 49
AvaII GGWCC 2 cut(s) 1449, 2163
BaeGI GKGCMC 3 cut(s) 94, 861, 1290
BaeI ACNNNNGTAYC 4 cut(s) 663, 696, 1458, 1491
BanI GGYRCC 2 cut(s) 89, 2216
BbrPI CACGTG 1 cut(s) 1372
BbsI GAAGAC 1 cut(s) 1259
Bbv12I GWGCWC 3 cut(s) 279, 861, 1290
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 49
BccI CCATC 4 cut(s) 404, 623, 1132, 1183
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 3 cut(s) 149, 896, 1222
BciVI GTATCC 1 cut(s) 730
BclI TGATCA 2 cut(s) 403, 1734
BcoDI GTCTC 4 cut(s) 113, 195, 494, 531
BcuI ACTAGT 1 cut(s) 391
BfaI CTAG 8 cut(s) 21, 50, 392, 1019, 1028, 1463, 1538, 1644
BfrI CTTAAG 1 cut(s) 1678
BfuI GTATCC 1 cut(s) 730
BisI GCNGC 2 cut(s) 63, 2066
BlsI GCNGC 2 cut(s) 64, 2067
BmcAI AGTACT 1 cut(s) 1322
Bme1390I CCNGG 3 cut(s) 149, 896, 1222
Bme18I GGWCC 2 cut(s) 1449, 2163
BmgT120I GGNCC 2 cut(s) 1449, 2163
BmiI GGNNCC 5 cut(s) 91, 802, 1008, 1730, 2218
BmrFI CCNGG 3 cut(s) 149, 896, 1222
BmsI GCATC 6 cut(s) 142, 214, 1518, 1687, 2064, 2126
BmtI GCTAGC 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 1259
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BpmI CTGGAG 4 cut(s) 131, 1913, 1937, 2042
Bpu10I CCTNAGC 2 cut(s) 228, 1012
Bsa29I ATCGAT 1 cut(s) 2229
BsaAI YACGTR 3 cut(s) 833, 1372, 1532
BsaHI GRCGYC 3 cut(s) 141, 204, 1798
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 894
BsaWI WCCGGW 1 cut(s) 1431
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 526, 556
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 621
Bse1I ACTGG 3 cut(s) 243, 519, 1896
Bse3DI GCAATG 2 cut(s) 2077, 2106
BseBI CCWGG 3 cut(s) 149, 896, 1222
BseCI ATCGAT 1 cut(s) 2229
BseDI CCNNGG 1 cut(s) 894
BseGI GGATG 6 cut(s) 157, 238, 1063, 1143, 2079, 2215
BseLI CCNNNNNNNGG 2 cut(s) 154, 621
BseMI GCAATG 2 cut(s) 2077, 2106
BseMII CTCAG 3 cut(s) 219, 2021, 2196
BseNI ACTGG 3 cut(s) 243, 519, 1896
BseRI GAGGAG 2 cut(s) 959, 1721
BseSI GKGCMC 3 cut(s) 94, 861, 1290
BseXI GCAGC 1 cut(s) 49
BseYI CCCAGC 1 cut(s) 2111
Bsh1285I CGRYCG 1 cut(s) 1436
BshFI GGCC 1 cut(s) 481
BshNI GGYRCC 2 cut(s) 89, 2216
BshVI ATCGAT 1 cut(s) 2229
BsiEI CGRYCG 1 cut(s) 1436
BsiHKAI GWGCWC 3 cut(s) 279, 861, 1290
BsiSI CCGG 2 cut(s) 1366, 1432
BslFI GGGAC 4 cut(s) 1112, 1326, 1400, 2165
BslI CCNNNNNNNGG 2 cut(s) 154, 621
BsmAI GTCTC 4 cut(s) 113, 195, 494, 531
BsmBI CGTCTC 2 cut(s) 195, 531
BsmFI GGGAC 4 cut(s) 1112, 1326, 1400, 2165
BsnI GGCC 1 cut(s) 481
Bso31I GGTCTC 1 cut(s) 113
Bsp1286I GDGCHC 4 cut(s) 94, 279, 861, 1290
Bsp143I GATC 6 cut(s) 403, 1479, 1734, 1863, 1957, 2169
BspACI CCGC 2 cut(s) 155, 2066
BspANI GGCC 1 cut(s) 481
BspCNI CTCAG 3 cut(s) 220, 2022, 2197
BspDI ATCGAT 1 cut(s) 2229
BspHI TCATGA 2 cut(s) 406, 1276
BspLI GGNNCC 5 cut(s) 91, 802, 1008, 1730, 2218
BspOI GCTAGC 1 cut(s) 53
BspPI GGATC 1 cut(s) 1487
BspT107I GGYRCC 2 cut(s) 89, 2216
BspTI CTTAAG 1 cut(s) 1678
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 2077, 2106
BsrI ACTGG 3 cut(s) 243, 519, 1896
BssECI CCNNGG 1 cut(s) 894
BssMI GATC 6 cut(s) 403, 1479, 1734, 1863, 1957, 2169
BssNI GRCGYC 3 cut(s) 141, 204, 1798
Bst2UI CCWGG 3 cut(s) 149, 896, 1222
Bst4CI ACNGT 6 cut(s) 298, 542, 1212, 1691, 1807, 1907
Bst6I CTCTTC 2 cut(s) 1398, 1854
BstACI GRCGYC 3 cut(s) 141, 204, 1798
BstAFI CTTAAG 1 cut(s) 1678
BstAPI GCANNNNNTGC 2 cut(s) 89, 2074
BstBAI YACGTR 3 cut(s) 833, 1372, 1532
BstC8I GCNNGC 2 cut(s) 51, 1658
BstDEI CTNAG 7 cut(s) 228, 1012, 1195, 1294, 1742, 2030, 2205
BstF5I GGATG 6 cut(s) 157, 238, 1063, 1143, 2079, 2215
BstHHI GCGC 1 cut(s) 1537
BstKTI GATC 6 cut(s) 406, 1482, 1737, 1866, 1960, 2172
BstMAI GTCTC 4 cut(s) 113, 195, 494, 531
BstMBI GATC 6 cut(s) 403, 1479, 1734, 1863, 1957, 2169
BstMCI CGRYCG 1 cut(s) 1436
BstMWI GCNNNNNNNGC 4 cut(s) 59, 89, 1191, 2074
BstNI CCWGG 3 cut(s) 149, 896, 1222
BstNSI RCATGY 2 cut(s) 1509, 2180
BstSCI CCNGG 3 cut(s) 147, 894, 1220
BstSLI GKGCMC 3 cut(s) 94, 861, 1290
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 1259
BstX2I RGATCY 1 cut(s) 1479
BstYI RGATCY 1 cut(s) 1479
Bsu15I ATCGAT 1 cut(s) 2229
BsuI GTATCC 1 cut(s) 730
BsuRI GGCC 1 cut(s) 481
BsuTUI ATCGAT 1 cut(s) 2229
BtgZI GCGATG 1 cut(s) 2001
BtsCI GGATG 6 cut(s) 157, 238, 1063, 1143, 2079, 2215
BtsI GCAGTG 1 cut(s) 2085
BtsIMutI CAGTG 5 cut(s) 666, 1120, 1311, 1803, 2085
Cac8I GCNNGC 2 cut(s) 51, 1658
CciI TCATGA 2 cut(s) 406, 1276
CfoI GCGC 1 cut(s) 1537
Cfr13I GGNCC 2 cut(s) 1449, 2163
ClaI ATCGAT 1 cut(s) 2229
CseI GACGC 2 cut(s) 25, 1806
Csp6I GTAC 5 cut(s) 236, 311, 1321, 1808, 1870
CspCI CAANNNNNGTGG 4 cut(s) 7, 42, 1287, 1322
CviAII CATG 9 cut(s) 7, 84, 407, 524, 907, 1277, 1506, 1982, 2177
CviQI GTAC 5 cut(s) 236, 311, 1321, 1808, 1870
DdeI CTNAG 7 cut(s) 228, 1012, 1195, 1294, 1742, 2030, 2205
DpnI GATC 6 cut(s) 405, 1481, 1736, 1865, 1959, 2171
DpnII GATC 6 cut(s) 403, 1479, 1734, 1863, 1957, 2169
DraI TTTAAA 2 cut(s) 438, 919
Eam1104I CTCTTC 2 cut(s) 1398, 1854
EarI CTCTTC 2 cut(s) 1398, 1854
Eco31I GGTCTC 1 cut(s) 113
Eco32I GATATC 2 cut(s) 1604, 2227
Eco47I GGWCC 2 cut(s) 1449, 2163
Eco57I CTGAAG 1 cut(s) 477
Eco72I CACGTG 1 cut(s) 1372
EcoRII CCWGG 3 cut(s) 147, 894, 1220
EcoRV GATATC 2 cut(s) 1604, 2227
EcoT22I ATGCAT 3 cut(s) 931, 1511, 2079
Esp3I CGTCTC 2 cut(s) 195, 531
FaeI CATG 9 cut(s) 10, 87, 410, 527, 910, 1280, 1509, 1985, 2180
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FaqI GGGAC 4 cut(s) 1112, 1326, 1400, 2165
FatI CATG 9 cut(s) 6, 83, 406, 523, 906, 1276, 1505, 1981, 2176
FbaI TGATCA 2 cut(s) 403, 1734
Fnu4HI GCNGC 2 cut(s) 63, 2066
FokI GGATG 6 cut(s) 164, 245, 1050, 1150, 2086, 2222
Fsp4HI GCNGC 2 cut(s) 63, 2066
FspBI CTAG 8 cut(s) 21, 50, 392, 1019, 1028, 1463, 1538, 1644
GlaI GCGC 1 cut(s) 1536
GluI GCNGC 2 cut(s) 63, 2066
GsaI CCCAGC 1 cut(s) 2115
GsuI CTGGAG 4 cut(s) 131, 1913, 1937, 2042
HaeIII GGCC 1 cut(s) 481
HapII CCGG 2 cut(s) 1366, 1432
HgaI GACGC 2 cut(s) 25, 1806
HhaI GCGC 1 cut(s) 1537
Hin1I GRCGYC 3 cut(s) 141, 204, 1798
Hin1II CATG 9 cut(s) 10, 87, 410, 527, 910, 1280, 1509, 1985, 2180
Hin6I GCGC 1 cut(s) 1535
HinP1I GCGC 1 cut(s) 1535
HincII GTYRAC 1 cut(s) 1889
HindII GTYRAC 1 cut(s) 1889
HpaII CCGG 2 cut(s) 1366, 1432
HphI GGTGA 3 cut(s) 316, 554, 1967
Hpy166II GTNNAC 5 cut(s) 313, 859, 1085, 1288, 1889
Hpy188I TCNGA 8 cut(s) 257, 266, 765, 1438, 1782, 1845, 1992, 2206
Hpy8I GTNNAC 5 cut(s) 313, 859, 1085, 1288, 1889
HpyAV CCTTC 4 cut(s) 512, 878, 1892, 2207
HpyCH4III ACNGT 6 cut(s) 298, 542, 1212, 1691, 1807, 1907
HpyCH4IV ACGT 5 cut(s) 141, 204, 832, 1371, 1531
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 89, 1191, 2074
HpyF3I CTNAG 7 cut(s) 228, 1012, 1195, 1294, 1742, 2030, 2205
HpySE526I ACGT 5 cut(s) 141, 204, 832, 1371, 1531
Hsp92I GRCGYC 3 cut(s) 141, 204, 1798
Hsp92II CATG 9 cut(s) 10, 87, 410, 527, 910, 1280, 1509, 1985, 2180
HspAI GCGC 1 cut(s) 1535
Ksp22I TGATCA 2 cut(s) 403, 1734
Kzo9I GATC 6 cut(s) 403, 1479, 1734, 1863, 1957, 2169
LmnI GCTCC 4 cut(s) 267, 800, 1054, 2023
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 6 cut(s) 142, 214, 1518, 1687, 2064, 2126
MaeI CTAG 8 cut(s) 21, 50, 392, 1019, 1028, 1463, 1538, 1644
MaeII ACGT 5 cut(s) 141, 204, 832, 1371, 1531
MaeIII GTNAC 7 cut(s) 195, 754, 823, 948, 1583, 1710, 1942
MalI GATC 6 cut(s) 405, 1481, 1736, 1865, 1959, 2171
MboI GATC 6 cut(s) 403, 1479, 1734, 1863, 1957, 2169
MboII GAAGA 8 cut(s) 184, 313, 950, 1187, 1264, 1415, 1639, 1871
MflI RGATCY 1 cut(s) 1479
MhlI GDGCHC 4 cut(s) 94, 279, 861, 1290
MlyI GAGTC 7 cut(s) 261, 500, 752, 956, 1422, 1928, 2195
MmeI TCCRAC 4 cut(s) 289, 702, 778, 1416
Mph1103I ATGCAT 3 cut(s) 931, 1511, 2079
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 9 cut(s) 135, 348, 371, 437, 918, 933, 1332, 1598, 1679
MspCI CTTAAG 1 cut(s) 1678
MspI CCGG 2 cut(s) 1366, 1432
MspR9I CCNGG 3 cut(s) 149, 896, 1222
MvaI CCWGG 3 cut(s) 149, 896, 1222
MwoI GCNNNNNNNGC 4 cut(s) 59, 89, 1191, 2074
NdeII GATC 6 cut(s) 403, 1479, 1734, 1863, 1957, 2169
NheI GCTAGC 1 cut(s) 49
NlaIII CATG 9 cut(s) 10, 87, 410, 527, 910, 1280, 1509, 1985, 2180
NlaIV GGNNCC 5 cut(s) 91, 802, 1008, 1730, 2218
NmuCI GTSAC 1 cut(s) 948
NsiI ATGCAT 3 cut(s) 931, 1511, 2079
NspI RCATGY 2 cut(s) 1509, 2180
PagI TCATGA 2 cut(s) 406, 1276
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 8 cut(s) 175, 334, 839, 902, 1518, 1591, 1616, 1840
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 2 cut(s) 64, 2067
PleI GAGTC 7 cut(s) 261, 500, 751, 955, 1421, 1927, 2195
PmaCI CACGTG 1 cut(s) 1372
PmlI CACGTG 1 cut(s) 1372
PpsI GAGTC 7 cut(s) 261, 500, 751, 955, 1421, 1927, 2195
Ppu21I YACGTR 3 cut(s) 833, 1372, 1532
PshBI ATTAAT 1 cut(s) 1332
Psp6I CCWGG 3 cut(s) 147, 894, 1220
PspCI CACGTG 1 cut(s) 1372
PspFI CCCAGC 1 cut(s) 2111
PspGI CCWGG 3 cut(s) 147, 894, 1220
PspN4I GGNNCC 5 cut(s) 91, 802, 1008, 1730, 2218
PspPI GGNCC 2 cut(s) 1449, 2163
PsuI RGATCY 1 cut(s) 1479
RsaI GTAC 5 cut(s) 237, 312, 1322, 1809, 1871
RsaNI GTAC 5 cut(s) 236, 311, 1321, 1808, 1870
SaqAI TTAA 9 cut(s) 135, 348, 371, 437, 918, 933, 1332, 1598, 1679
SatI GCNGC 2 cut(s) 63, 2066
Sau3AI GATC 6 cut(s) 403, 1479, 1734, 1863, 1957, 2169
Sau96I GGNCC 2 cut(s) 1449, 2163
ScaI AGTACT 1 cut(s) 1322
SchI GAGTC 7 cut(s) 261, 500, 752, 956, 1422, 1928, 2195
ScrFI CCNGG 3 cut(s) 149, 896, 1222
SduI GDGCHC 4 cut(s) 94, 279, 861, 1290
SfaNI GCATC 6 cut(s) 142, 214, 1518, 1687, 2064, 2126
SinI GGWCC 2 cut(s) 1449, 2163
SpeI ACTAGT 1 cut(s) 391
SsiI CCGC 2 cut(s) 155, 2066
SspI AATATT 1 cut(s) 1493
SspMI CTAG 8 cut(s) 21, 50, 392, 1019, 1028, 1463, 1538, 1644
StyD4I CCNGG 3 cut(s) 147, 894, 1220
TaaI ACNGT 6 cut(s) 298, 542, 1212, 1691, 1807, 1907
TaiI ACGT 5 cut(s) 144, 207, 835, 1374, 1534
TaqI TCGA 4 cut(s) 594, 746, 1866, 2229
TatI WGTACW 3 cut(s) 235, 1320, 1807
TauI GCSGC 1 cut(s) 2068
TfiI GAWTC 8 cut(s) 175, 334, 839, 902, 1518, 1591, 1616, 1840
Tru1I TTAA 9 cut(s) 135, 348, 371, 437, 918, 933, 1332, 1598, 1679
Tru9I TTAA 9 cut(s) 135, 348, 371, 437, 918, 933, 1332, 1598, 1679
TscAI CASTG 5 cut(s) 666, 1120, 1311, 1810, 2092
TseFI GTSAC 1 cut(s) 948
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 948
TspDTI ATGAA 9 cut(s) 852, 983, 1119, 1134, 1188, 1265, 1414, 1583, 2226
TspGWI ACGGA 2 cut(s) 551, 1462
TspRI CASTG 5 cut(s) 666, 1120, 1311, 1810, 2092
Vha464I CTTAAG 1 cut(s) 1678
VneI GTGCAC 2 cut(s) 857, 1286
VpaK11BI GGWCC 2 cut(s) 1449, 2163
VspI ATTAAT 1 cut(s) 1332
XapI RAATTY 6 cut(s) 11, 555, 689, 1106, 1272, 1666
XbaI TCTAGA 1 cut(s) 1462
XceI RCATGY 2 cut(s) 1509, 2180
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 8 cut(s) 21, 50, 392, 1019, 1028, 1463, 1538, 1644
ZraI GACGTC 2 cut(s) 142, 205
ZrmI AGTACT 1 cut(s) 1322
Zsp2I ATGCAT 3 cut(s) 931, 1511, 2079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.