RchiOBHm_Chr7g0196041

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008133
Physical Location & Seq
Forward (+)
13908632 .. 13909135
504 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17533

Sequence Viewer

Length: 504 bp
ATGGCTAGTTCAACACCAATTTCAGCGCTTTTCCTCTTCTTCCTCCTCGCATCTGGCTCTGCGGCCACAGCACCCAGCAAGAGAAGGTTGTTGTTTTACTTCAATGACATTCTTTACGATGGAAAGAATGCATACAGAACCACTTTGGCAGGAGAAAACCATTTGGGTGACATGGTCGTCTTTGATGACCCCGTTAACTTGGACAACGATCTGCAGCCAACCCAAGTTCGTTGTGCACAAAGCTTATGTTTTTATGGCAAGAGAGATATAATCACTGCTTGGCTTGGCTTCTCCTTCGTGTTTAACTACACCAAGCGCACTGGAGGAAGCTTCAACTTTCCTGGTGCTAATCCTTTGATGGGCAAGGCCGAGGACATTTCAGTGATTGGTGGTACTGGTGATTTCTTCATGGGTCAAGGGAGGGCAATTTTGATGATTAATTCATTTAAGGGGAAACTGTACTTCAGGCTTCGCCTTGATTTTAATCTTTATGAGAGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

167

Amino Acids

18.67

Weight (kDa)

8.56

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 29 - 150 9.3e-21 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AciI CCGC 1 cut(s) 62
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 448
AfaI GTAC 2 cut(s) 394, 461
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 359
AgsI TTSAA 3 cut(s) 12, 103, 334
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 3 cut(s) 243, 330, 498
AluI AGCT 3 cut(s) 243, 330, 498
Alw21I GWGCWC 1 cut(s) 238
Alw44I GTGCAC 1 cut(s) 234
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 2 cut(s) 63, 366
ApaLI GTGCAC 1 cut(s) 234
ApeKI GCWGC 1 cut(s) 214
AseI ATTAAT 1 cut(s) 438
AspLEI GCGC 2 cut(s) 28, 318
AsuHPI GGTGA 2 cut(s) 179, 410
BaeGI GKGCMC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 1 cut(s) 226
BccI CCATC 2 cut(s) 113, 352
BciT130I CCWGG 1 cut(s) 342
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 212
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 2 cut(s) 63, 215
BlsI GCNGC 2 cut(s) 64, 216
Bme1390I CCNGG 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 59
BpmI CTGGAG 1 cut(s) 342
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 325, 400
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 369
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 325, 400
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 238
BseXI GCAGC 1 cut(s) 226
BseYI CCCAGC 1 cut(s) 74
BshFI GGCC 2 cut(s) 65, 368
BsiHKAI GWGCWC 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 359
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 2 cut(s) 65, 368
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 1 cut(s) 62
BspANI GGCC 2 cut(s) 65, 368
BspMAI CTGCAG 1 cut(s) 216
BsrI ACTGG 2 cut(s) 325, 400
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 1 cut(s) 208
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 459
Bst6I CTCTTC 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 318
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 212
BstSLI GKGCMC 1 cut(s) 238
BstV1I GCAGC 1 cut(s) 226
BsuRI GGCC 2 cut(s) 65, 368
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 273, 318, 387
CfoI GCGC 2 cut(s) 28, 318
Csp6I GTAC 2 cut(s) 393, 460
CviAII CATG 2 cut(s) 172, 409
CviQI GTAC 2 cut(s) 393, 460
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco47III AGCGCT 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 448
EcoRII CCWGG 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 133
FaeI CATG 2 cut(s) 175, 412
FaiI YATR 7 cut(s) 133, 173, 247, 255, 269, 410, 492
FatI CATG 2 cut(s) 171, 408
Fnu4HI GCNGC 2 cut(s) 63, 215
Fsp4HI GCNGC 2 cut(s) 63, 215
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 27, 317
GluI GCNGC 2 cut(s) 63, 215
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 2 cut(s) 65, 368
HhaI GCGC 2 cut(s) 28, 318
Hin1II CATG 2 cut(s) 175, 412
Hin6I GCGC 2 cut(s) 26, 316
HinP1I GCGC 2 cut(s) 26, 316
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HindIII AAGCTT 2 cut(s) 241, 328
HpaI GTTAAC 1 cut(s) 196
HphI GGTGA 2 cut(s) 179, 410
Hpy166II GTNNAC 2 cut(s) 196, 236
Hpy8I GTNNAC 2 cut(s) 196, 236
HpyAV CCTTC 2 cut(s) 78, 304
HpyCH4III ACNGT 1 cut(s) 459
HpyCH4V TGCA 3 cut(s) 131, 214, 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
Hsp92II CATG 2 cut(s) 175, 412
HspAI GCGC 2 cut(s) 26, 316
KspAI GTTAAC 1 cut(s) 196
Kzo9I GATC 1 cut(s) 208
LpnPI CCDG 9 cut(s) 39, 88, 135, 306, 327, 354, 381, 451, 484
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 1 cut(s) 59
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 167
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 3 cut(s) 28, 31, 397
MhlI GDGCHC 1 cut(s) 238
MluCI AATT 3 cut(s) 18, 426, 439
MnlI CCTC 6 cut(s) 44, 53, 56, 317, 364, 414
Mph1103I ATGCAT 1 cut(s) 133
MseI TTAA 5 cut(s) 195, 303, 438, 447, 483
MslI CAYNNNNRTG 2 cut(s) 165, 380
MspR9I CCNGG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 1 cut(s) 208
NlaIII CATG 2 cut(s) 175, 412
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 167
NsiI ATGCAT 1 cut(s) 133
PctI GAATGC 1 cut(s) 133
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 2 cut(s) 64, 216
PshBI ATTAAT 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 340
PstI CTGCAG 1 cut(s) 216
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 2 cut(s) 394, 461
RsaNI GTAC 2 cut(s) 393, 460
RseI CAYNNNNRTG 2 cut(s) 165, 380
SaqAI TTAA 5 cut(s) 195, 303, 438, 447, 483
SatI GCNGC 2 cut(s) 63, 215
Sau3AI GATC 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 238
SetI ASST 4 cut(s) 89, 245, 332, 500
SfaNI GCATC 1 cut(s) 59
SfcI CTRYAG 1 cut(s) 212
SmiMI CAYNNNNRTG 2 cut(s) 165, 380
Sse9I AATT 3 cut(s) 18, 426, 439
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 459
TasI AATT 3 cut(s) 18, 426, 439
TatI WGTACW 1 cut(s) 459
TauI GCSGC 1 cut(s) 65
Tru1I TTAA 5 cut(s) 195, 303, 438, 447, 483
Tru9I TTAA 5 cut(s) 195, 303, 438, 447, 483
TscAI CASTG 3 cut(s) 280, 325, 387
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 1 cut(s) 214
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 2 cut(s) 397, 432
TspRI CASTG 3 cut(s) 280, 325, 387
Tth111I GACNNNGTC 1 cut(s) 173
VneI GTGCAC 1 cut(s) 234
VspI ATTAAT 1 cut(s) 438
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.