Rh7AG155900

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008133
Physical Location & Seq
Forward (+)
12687866 .. 12688274
409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG155900.1

Sequence Viewer

Length: 405 bp
ATGGCTAGTTCAACACCAATTTCAGCGCTTTTCCTCTTCTTCCTCCTCGCATCTGGCTCTGCGGCCACAGCACCCAGCAAGAGAAGGTTGGTGTTTTACTTCAATGACATTTACGATGGAAAGAATGCATACAGAACCACTTTGGCAGGAGAAAACCATTTGGGTGACATGGTCGTCTCTTGGCTTGGCTTCTCCTTCGTGTTTAACTACACCAAGCGCACTGGAGGAAGCTTCAACTTTCCTGGTGCTAATCCTTTGATGGGCAAGGCCGAGGACATTTCAGTGATTGGTGGTACTGGTGATTTCTTCATGGGTCAAGGGAGGGCAATTTTGATGATTAATTCATTTCAGGGGAAACTGTACTTCAGGCTTCGCCTTGATTTTAATCTTTATGAGAGTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

134

Amino Acids

14.89

Weight (kDa)

9.34

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 59 - 117 3.7e-11 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AciI CCGC 1 cut(s) 62
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 349
AfaI GTAC 2 cut(s) 295, 362
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 260
AgsI TTSAA 3 cut(s) 12, 103, 235
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 1 cut(s) 231
AluI AGCT 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 181
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 2 cut(s) 63, 267
AseI ATTAAT 1 cut(s) 339
AspLEI GCGC 2 cut(s) 28, 219
AsuHPI GGTGA 2 cut(s) 176, 311
BccI CCATC 2 cut(s) 110, 253
BciT130I CCWGG 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 243
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 1 cut(s) 59
BpmI CTGGAG 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 270
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 226, 301
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 270
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 1 cut(s) 260
BseNI ACTGG 2 cut(s) 226, 301
BseRI GAGGAG 1 cut(s) 35
BseYI CCCAGC 1 cut(s) 74
BshFI GGCC 2 cut(s) 65, 269
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 181
BsmBI CGTCTC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 2 cut(s) 65, 269
BspACI CCGC 1 cut(s) 62
BspANI GGCC 2 cut(s) 65, 269
BsrI ACTGG 2 cut(s) 226, 301
BssECI CCNNGG 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 360
Bst6I CTCTTC 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 219
BstMAI GTCTC 1 cut(s) 181
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BsuRI GGCC 2 cut(s) 65, 269
BtsIMutI CAGTG 2 cut(s) 219, 288
CfoI GCGC 2 cut(s) 28, 219
Csp6I GTAC 2 cut(s) 294, 361
CviAII CATG 2 cut(s) 169, 310
CviJI RGCY 8 cut(s) 5, 57, 65, 184, 189, 231, 269, 370
CviKI_1 RGCY 8 cut(s) 5, 57, 65, 184, 189, 231, 269, 370
CviQI GTAC 2 cut(s) 294, 361
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco47III AGCGCT 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 2 cut(s) 172, 313
FaiI YATR 4 cut(s) 130, 170, 311, 393
FatI CATG 2 cut(s) 168, 309
Fnu4HI GCNGC 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 27, 218
GluI GCNGC 1 cut(s) 63
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 243
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 2 cut(s) 65, 269
HhaI GCGC 2 cut(s) 28, 219
Hin1II CATG 2 cut(s) 172, 313
Hin6I GCGC 2 cut(s) 26, 217
HinP1I GCGC 2 cut(s) 26, 217
HindIII AAGCTT 1 cut(s) 229
HphI GGTGA 2 cut(s) 176, 311
HpyAV CCTTC 2 cut(s) 78, 205
HpyCH4III ACNGT 1 cut(s) 360
HpyCH4V TGCA 1 cut(s) 128
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
Hsp92II CATG 2 cut(s) 172, 313
HspAI GCGC 2 cut(s) 26, 217
LpnPI CCDG 9 cut(s) 39, 88, 132, 207, 228, 255, 282, 335, 352
LweI GCATC 1 cut(s) 59
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 164
MboII GAAGA 3 cut(s) 28, 31, 298
MluCI AATT 3 cut(s) 18, 327, 340
MnlI CCTC 6 cut(s) 44, 53, 56, 218, 265, 315
Mph1103I ATGCAT 1 cut(s) 130
MseI TTAA 3 cut(s) 204, 339, 384
MslI CAYNNNNRTG 2 cut(s) 162, 281
MspR9I CCNGG 1 cut(s) 243
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 68
NlaIII CATG 2 cut(s) 172, 313
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 164
NsiI ATGCAT 1 cut(s) 130
PctI GAATGC 1 cut(s) 130
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 1 cut(s) 64
PshBI ATTAAT 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 2 cut(s) 295, 362
RsaNI GTAC 2 cut(s) 294, 361
RseI CAYNNNNRTG 2 cut(s) 162, 281
SaqAI TTAA 3 cut(s) 204, 339, 384
SatI GCNGC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 243
SetI ASST 2 cut(s) 89, 233
SfaNI GCATC 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 162, 281
Sse9I AATT 3 cut(s) 18, 327, 340
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 360
TasI AATT 3 cut(s) 18, 327, 340
TatI WGTACW 1 cut(s) 360
TauI GCSGC 1 cut(s) 65
Tru1I TTAA 3 cut(s) 204, 339, 384
Tru9I TTAA 3 cut(s) 204, 339, 384
TscAI CASTG 2 cut(s) 226, 288
TseFI GTSAC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 2 cut(s) 298, 333
TspRI CASTG 2 cut(s) 226, 288
Tth111I GACNNNGTC 1 cut(s) 170
VspI ATTAAT 1 cut(s) 339
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.