Rmu_sc0001640.1_g000010

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001640.1
OG0008133
Physical Location & Seq
Reverse (-)
46664 .. 47167
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001640.1_g000010.1.cds

Sequence Viewer

Length: 504 bp
atggctagttcaacgccaatttcagcgcttttcctcttcttcctcctcgcatctggctctgcggccacagcacccagcaagagaaggttgttgttttacttcaatgacattctttacgatggaaagaatgcatacagaaccactttggcaggagaaaaccatttgggtgacatggtcgtctttgatgaccctgttaacttggacaacgatttgcagtcaacccaagttcgttgtgcacaaagcttatgtttttatggcaagagagataaaatcactgcttggcttggcttctccttcgtgtttaactacaccaagcgcactggaggaagcttcaactttcctggtgctaatcctttgatgggcaaggccgaggacatttcagttattggtggtactggtgatttcttcatgggtcaagggagggcaattttgatgattaattcatttcaggggaaactgtacttcaggcttcgccttgattttaatctttatgagagttggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

167

Amino Acids

18.67

Weight (kDa)

8.56

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AciI CCGC 1 cut(s) 62
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 448
AfaI GTAC 2 cut(s) 394, 461
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 359
AgsI TTSAA 3 cut(s) 12, 103, 334
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 2 cut(s) 243, 330
AluI AGCT 2 cut(s) 243, 330
Alw21I GWGCWC 1 cut(s) 238
Alw44I GTGCAC 1 cut(s) 234
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 2 cut(s) 63, 366
ApaLI GTGCAC 1 cut(s) 234
AseI ATTAAT 1 cut(s) 438
AspLEI GCGC 2 cut(s) 28, 318
AsuHPI GGTGA 2 cut(s) 179, 410
BaeGI GKGCMC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 238
BccI CCATC 2 cut(s) 113, 352
BciT130I CCWGG 1 cut(s) 342
BfaI CTAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 59
BpmI CTGGAG 1 cut(s) 342
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 325, 400
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 369
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 325, 400
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 238
BseYI CCCAGC 1 cut(s) 74
BshFI GGCC 2 cut(s) 65, 368
BsiHKAI GWGCWC 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 359
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 2 cut(s) 65, 368
Bsp1286I GDGCHC 1 cut(s) 238
BspACI CCGC 1 cut(s) 62
BspANI GGCC 2 cut(s) 65, 368
BsrI ACTGG 2 cut(s) 325, 400
BssECI CCNNGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 459
Bst6I CTCTTC 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 318
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstSLI GKGCMC 1 cut(s) 238
BsuRI GGCC 2 cut(s) 65, 368
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 273, 318
CfoI GCGC 2 cut(s) 28, 318
Csp6I GTAC 2 cut(s) 393, 460
CviAII CATG 2 cut(s) 172, 409
CviJI RGCY 9 cut(s) 5, 57, 65, 243, 283, 288, 330, 368, 469
CviKI_1 RGCY 9 cut(s) 5, 57, 65, 243, 283, 288, 330, 368, 469
CviQI GTAC 2 cut(s) 393, 460
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco47III AGCGCT 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 448
EcoRII CCWGG 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 133
FaeI CATG 2 cut(s) 175, 412
FaiI YATR 6 cut(s) 133, 173, 247, 255, 410, 492
FatI CATG 2 cut(s) 171, 408
Fnu4HI GCNGC 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 27, 317
GluI GCNGC 1 cut(s) 63
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 2 cut(s) 65, 368
HhaI GCGC 2 cut(s) 28, 318
Hin1II CATG 2 cut(s) 175, 412
Hin6I GCGC 2 cut(s) 26, 316
HinP1I GCGC 2 cut(s) 26, 316
HincII GTYRAC 2 cut(s) 196, 219
HindII GTYRAC 2 cut(s) 196, 219
HindIII AAGCTT 2 cut(s) 241, 328
HpaI GTTAAC 1 cut(s) 196
HphI GGTGA 2 cut(s) 179, 410
Hpy166II GTNNAC 3 cut(s) 196, 219, 236
Hpy8I GTNNAC 3 cut(s) 196, 219, 236
HpyAV CCTTC 2 cut(s) 78, 304
HpyCH4III ACNGT 1 cut(s) 459
HpyCH4V TGCA 3 cut(s) 131, 214, 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
Hsp92II CATG 2 cut(s) 175, 412
HspAI GCGC 2 cut(s) 26, 316
KspAI GTTAAC 1 cut(s) 196
LweI GCATC 1 cut(s) 59
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 167
MboII GAAGA 3 cut(s) 28, 31, 397
MhlI GDGCHC 1 cut(s) 238
MluCI AATT 3 cut(s) 18, 426, 439
MnlI CCTC 6 cut(s) 44, 53, 56, 317, 364, 414
Mph1103I ATGCAT 1 cut(s) 133
MseI TTAA 4 cut(s) 195, 303, 438, 483
MslI CAYNNNNRTG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 68
NlaIII CATG 2 cut(s) 175, 412
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 167
NsiI ATGCAT 1 cut(s) 133
PctI GAATGC 1 cut(s) 133
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 1 cut(s) 64
PshBI ATTAAT 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 340
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 2 cut(s) 394, 461
RsaNI GTAC 2 cut(s) 393, 460
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 4 cut(s) 195, 303, 438, 483
SatI GCNGC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 238
SetI ASST 3 cut(s) 89, 245, 332
SfaNI GCATC 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 165
Sse9I AATT 3 cut(s) 18, 426, 439
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 459
TasI AATT 3 cut(s) 18, 426, 439
TatI WGTACW 1 cut(s) 459
TauI GCSGC 1 cut(s) 65
Tru1I TTAA 4 cut(s) 195, 303, 438, 483
Tru9I TTAA 4 cut(s) 195, 303, 438, 483
TscAI CASTG 2 cut(s) 280, 325
TseFI GTSAC 1 cut(s) 167
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 2 cut(s) 397, 432
TspRI CASTG 2 cut(s) 280, 325
Tth111I GACNNNGTC 1 cut(s) 173
VneI GTGCAC 1 cut(s) 234
VspI ATTAAT 1 cut(s) 438
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.