RLG00000004071

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008133
Physical Location & Seq
Reverse (-)
55854704 .. 55855201
498 bp
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UTR
Exon/CDS
Intron
RLM00000004071

Sequence Viewer

Length: 498 bp
ATGGCTAGTTCAACACCAATTTCAGCGCTTTTCCTCTTCTTCCTCCTCGCATCTGGCTCTGCGGCCACAGCACCCAGCAAGAGAAGGTTGGTGATTTACTTCAATGATGTTCTTTACGATGGAAAGAATGCAAACAGAACCACTTTGGCAGGAGAAAACCCTTTGGGTGACATGGTCGTCTTTGATGACCCCGTTAACTTGGACAACGATCTGCAGTCAACCCAAGTTCGTTGTGCACAAAGCTTATGTTTCTATGGCAAGAGAGATATATTCACTGCTTGGCTTGGCTTCTTCGTGTTTAACTACACCAAGCGCACTGGAGGAAGCTTCAACTTTTCTGGTGCTAATCCTTTGATGGCCAAGGCCGAGGACATTTCAGTGATTGGTGGTACTGGTGATTTCTTCATGGGCCAAGGGAGGGCAATTTTGATGATTAATTCATTTCAGGGGAAACTGTACTTCAGGCTTCGCCTTGATTTTAATCTTTATGAGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

166

Amino Acids

18.27

Weight (kDa)

7.72

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 29 - 149 4.6e-20 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AciI CCGC 1 cut(s) 62
AcoI YGGCCR 2 cut(s) 63, 357
AcuI CTGAAG 1 cut(s) 445
AfaI GTAC 2 cut(s) 391, 458
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 3 cut(s) 12, 103, 331
AluBI AGCT 2 cut(s) 243, 327
AluI AGCT 2 cut(s) 243, 327
Alw21I GWGCWC 1 cut(s) 238
Alw44I GTGCAC 1 cut(s) 234
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 4 cut(s) 63, 357, 363, 409
ApaLI GTGCAC 1 cut(s) 234
AseI ATTAAT 1 cut(s) 435
AspLEI GCGC 2 cut(s) 28, 315
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 3 cut(s) 103, 179, 407
BaeGI GKGCMC 1 cut(s) 238
BalI TGGCCA 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 238
BccI CCATC 2 cut(s) 113, 349
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 212
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 409
BmsI GCATC 1 cut(s) 59
BpmI CTGGAG 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 3 cut(s) 360, 366, 412
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 322, 397
Bse8I GATNNNNATC 1 cut(s) 480
BseDI CCNNGG 3 cut(s) 360, 366, 412
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 418
BseNI ACTGG 2 cut(s) 322, 397
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 238
BseYI CCCAGC 1 cut(s) 74
BshFI GGCC 4 cut(s) 65, 359, 365, 411
BsiHKAI GWGCWC 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 418
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 4 cut(s) 65, 359, 365, 411
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 1 cut(s) 62
BspANI GGCC 4 cut(s) 65, 359, 365, 411
BspMAI CTGCAG 1 cut(s) 216
BsrI ACTGG 2 cut(s) 322, 397
BssECI CCNNGG 3 cut(s) 360, 366, 412
BssMI GATC 1 cut(s) 208
BssT1I CCWWGG 2 cut(s) 360, 412
Bst4CI ACNGT 1 cut(s) 456
Bst6I CTCTTC 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 315
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstSFI CTRYAG 1 cut(s) 212
BstSLI GKGCMC 1 cut(s) 238
BsuRI GGCC 4 cut(s) 65, 359, 365, 411
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 273, 315, 384
CfoI GCGC 2 cut(s) 28, 315
Cfr13I GGNCC 1 cut(s) 409
Csp6I GTAC 2 cut(s) 390, 457
CviAII CATG 2 cut(s) 172, 406
CviQI GTAC 2 cut(s) 390, 457
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 2 cut(s) 63, 357
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 360, 412
Eco47III AGCGCT 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 445
EcoT14I CCWWGG 2 cut(s) 360, 412
ErhI CCWWGG 2 cut(s) 360, 412
FaeI CATG 2 cut(s) 175, 409
FaiI YATR 6 cut(s) 173, 247, 255, 269, 407, 489
FatI CATG 2 cut(s) 171, 405
Fnu4HI GCNGC 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 27, 314
GluI GCNGC 1 cut(s) 63
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 4 cut(s) 65, 359, 365, 411
HhaI GCGC 2 cut(s) 28, 315
Hin1II CATG 2 cut(s) 175, 409
Hin6I GCGC 2 cut(s) 26, 313
HinP1I GCGC 2 cut(s) 26, 313
HincII GTYRAC 2 cut(s) 196, 219
HindII GTYRAC 2 cut(s) 196, 219
HindIII AAGCTT 2 cut(s) 241, 325
HpaI GTTAAC 1 cut(s) 196
HphI GGTGA 3 cut(s) 103, 179, 407
Hpy166II GTNNAC 3 cut(s) 196, 219, 236
Hpy8I GTNNAC 3 cut(s) 196, 219, 236
HpyAV CCTTC 1 cut(s) 78
HpyCH4III ACNGT 1 cut(s) 456
HpyCH4V TGCA 3 cut(s) 131, 214, 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
Hsp92II CATG 2 cut(s) 175, 409
HspAI GCGC 2 cut(s) 26, 313
KspAI GTTAAC 1 cut(s) 196
Kzo9I GATC 1 cut(s) 208
LpnPI CCDG 8 cut(s) 39, 88, 135, 303, 324, 378, 431, 448
LweI GCATC 1 cut(s) 59
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 167
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 4 cut(s) 28, 31, 283, 394
MhlI GDGCHC 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 3 cut(s) 18, 423, 436
MluNI TGGCCA 1 cut(s) 359
MnlI CCTC 6 cut(s) 44, 53, 56, 314, 361, 411
Mox20I TGGCCA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 359
MseI TTAA 4 cut(s) 195, 300, 435, 480
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 359
Mva1269I GAATGC 1 cut(s) 133
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 1 cut(s) 208
NlaIII CATG 2 cut(s) 175, 409
NmeAIII GCCGAG 1 cut(s) 391
NmuCI GTSAC 1 cut(s) 167
PctI GAATGC 1 cut(s) 133
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 1 cut(s) 64
PshBI ATTAAT 1 cut(s) 435
PspFI CCCAGC 1 cut(s) 74
PspPI GGNCC 1 cut(s) 409
PstI CTGCAG 1 cut(s) 216
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 2 cut(s) 391, 458
RsaNI GTAC 2 cut(s) 390, 457
RseI CAYNNNNRTG 1 cut(s) 377
SaqAI TTAA 4 cut(s) 195, 300, 435, 480
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 1 cut(s) 409
SduI GDGCHC 1 cut(s) 238
SetI ASST 3 cut(s) 89, 245, 329
SfaNI GCATC 1 cut(s) 59
SfcI CTRYAG 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 377
Sse9I AATT 3 cut(s) 18, 423, 436
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 6
StyI CCWWGG 2 cut(s) 360, 412
TaaI ACNGT 1 cut(s) 456
TasI AATT 3 cut(s) 18, 423, 436
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 65
Tru1I TTAA 4 cut(s) 195, 300, 435, 480
Tru9I TTAA 4 cut(s) 195, 300, 435, 480
TscAI CASTG 3 cut(s) 280, 322, 384
TseFI GTSAC 1 cut(s) 167
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 2 cut(s) 394, 429
TspRI CASTG 3 cut(s) 280, 322, 384
Tth111I GACNNNGTC 1 cut(s) 173
VneI GTGCAC 1 cut(s) 234
VspI ATTAAT 1 cut(s) 435
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.