RLG00000004072

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008133
Physical Location & Seq
Reverse (-)
55856931 .. 55857997
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004072

Sequence Viewer

Length: 576 bp
ATGGAATCAAAGAGTCTAGCTTCAACTCTCTTCCTCTTCTTCCTTCTCTTTGGAACCTTAGCAGCTTCCCCCACCCTCAAATCGAAGCCTCATCCTCGCCGTCCTCTCCCTTGCAAACGCCTCGTTTTCTATTTCCACGATATTGTCTACAACGGCAAGAATTCCAAGAATGCCACAGCGGCGATTGTGGGTTCACCCGCTTGGGGCAACAAGACCATATTGGCAGGGAAGAGCCATTTTGGGAATGTGGTTGTGTTCGACGATCCAATCACTTTGGACAACAATTTGCACTCGACCCCGGTTGGTCGTGCGCAAGGCTTTTACTTGTACGACAAGAAAGATATATTCACTGCTTGGCTCGGCTTCTCCTTCGTGTTCAACTCGACCCAACACAAAGGGAGCATCAATTTTGCTGGGGCTGATCCATTGATGAACAAGACTCGAGACATTTCAGTTATTGGTGGAACTGGTGACTTCTTCATGACTAGAGGGATCGCCACTTTGATGACTGATGCATTTGAGGGTGAAGTCTATTTCCGTCTCAAAGTTGACATTAAACTGTACGAGTGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

192

Amino Acids

21.28

Weight (kDa)

9.34

Isoelectric Point (pI)

21.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 38 - 184 2.1e-38 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 312
AccI GTMKAC 1 cut(s) 147
AciI CCGC 2 cut(s) 179, 198
AclWI GGATC 3 cut(s) 257, 416, 500
AcsI RAATTY 1 cut(s) 160
AfaI GTAC 2 cut(s) 329, 563
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 2 cut(s) 24, 379
AluBI AGCT 2 cut(s) 20, 65
AluI AGCT 2 cut(s) 20, 65
Alw26I GTCTC 2 cut(s) 438, 545
AlwI GGATC 3 cut(s) 257, 416, 500
Ama87I CYCGRG 1 cut(s) 441
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 1 cut(s) 160
AspLEI GCGC 1 cut(s) 313
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 3 cut(s) 186, 482, 536
AvaI CYCGRG 1 cut(s) 441
BbvI GCAGC 1 cut(s) 74
BceAI ACGGC 2 cut(s) 84, 169
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 438, 545
BfaI CTAG 2 cut(s) 17, 486
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 2 cut(s) 63, 180
BlsI GCNGC 2 cut(s) 64, 181
Bme1390I CCNGG 1 cut(s) 299
BmeT110I CYCGRG 1 cut(s) 441
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 299
BmsI GCATC 2 cut(s) 411, 502
Bpu10I CCTNAGC 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 297
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 472
BseDI CCNNGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 472
BseXI GCAGC 1 cut(s) 74
BseYI CCCAGC 1 cut(s) 413
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 2 cut(s) 438, 545
BsmBI CGTCTC 1 cut(s) 545
BsmI GAATGC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 441
Bsp143I GATC 3 cut(s) 262, 421, 492
BspACI CCGC 2 cut(s) 179, 198
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 3 cut(s) 257, 416, 500
BspQI GCTCTTC 1 cut(s) 224
BsrI ACTGG 1 cut(s) 472
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 3 cut(s) 262, 421, 492
Bst4CI ACNGT 1 cut(s) 561
Bst6I CTCTTC 3 cut(s) 35, 41, 224
BstDEI CTNAG 1 cut(s) 58
BstF5I GGATG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 3 cut(s) 265, 424, 495
BstMAI GTCTC 2 cut(s) 438, 545
BstMBI GATC 3 cut(s) 262, 421, 492
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 74
BtsCI GGATG 1 cut(s) 91
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 348
CciI TCATGA 1 cut(s) 480
CfoI GCGC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 328, 562
CviAII CATG 1 cut(s) 481
CviJI RGCY 8 cut(s) 20, 65, 88, 234, 318, 358, 363, 419
CviKI_1 RGCY 8 cut(s) 20, 65, 88, 234, 318, 358, 363, 419
CviQI GTAC 2 cut(s) 328, 562
DdeI CTNAG 1 cut(s) 58
DpnI GATC 3 cut(s) 264, 423, 494
DpnII GATC 3 cut(s) 262, 421, 492
Eam1104I CTCTTC 3 cut(s) 35, 41, 224
EarI CTCTTC 3 cut(s) 35, 41, 224
Eco88I CYCGRG 1 cut(s) 441
EcoRI GAATTC 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 517
Esp3I CGTCTC 1 cut(s) 545
FaeI CATG 1 cut(s) 484
FaiI YATR 3 cut(s) 218, 344, 482
FatI CATG 1 cut(s) 480
FauI CCCGC 1 cut(s) 205
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 2 cut(s) 63, 180
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 2 cut(s) 63, 180
FspBI CTAG 2 cut(s) 17, 486
FspI TGCGCA 1 cut(s) 312
GlaI GCGC 1 cut(s) 312
GluI GCNGC 2 cut(s) 63, 180
GsaI CCCAGC 1 cut(s) 417
HapII CCGG 1 cut(s) 299
HhaI GCGC 1 cut(s) 313
Hin1II CATG 1 cut(s) 484
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HinfI GANTC 3 cut(s) 5, 13, 439
HpaII CCGG 1 cut(s) 299
HphI GGTGA 3 cut(s) 186, 482, 536
Hpy166II GTNNAC 3 cut(s) 148, 194, 550
Hpy188III TCNNGA 2 cut(s) 443, 481
Hpy8I GTNNAC 3 cut(s) 148, 194, 550
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 53, 379
HpyCH4III ACNGT 1 cut(s) 561
HpyCH4V TGCA 3 cut(s) 114, 289, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 1 cut(s) 58
Hsp92II CATG 1 cut(s) 484
HspAI GCGC 1 cut(s) 311
Kzo9I GATC 3 cut(s) 262, 421, 492
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 1 cut(s) 399
LpnPI CCDG 4 cut(s) 210, 312, 399, 453
Lsp1109I GCAGC 1 cut(s) 74
LweI GCATC 2 cut(s) 411, 502
MaeI CTAG 2 cut(s) 17, 486
MaeIII GTNAC 1 cut(s) 470
MalI GATC 3 cut(s) 264, 423, 494
MboI GATC 3 cut(s) 262, 421, 492
MboII GAAGA 5 cut(s) 22, 28, 31, 241, 469
MluCI AATT 3 cut(s) 160, 283, 406
MlyI GAGTC 2 cut(s) 22, 433
MnlI CCTC 8 cut(s) 44, 86, 99, 105, 114, 131, 482, 514
Mph1103I ATGCAT 1 cut(s) 517
MseI TTAA 1 cut(s) 555
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 1 cut(s) 299
Mva1269I GAATGC 1 cut(s) 175
MwoI GCNNNNNNNGC 1 cut(s) 179
NciI CCSGG 1 cut(s) 299
NdeII GATC 3 cut(s) 262, 421, 492
NlaIII CATG 1 cut(s) 484
NlaIV GGNNCC 1 cut(s) 55
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 470
NsbI TGCGCA 1 cut(s) 312
NsiI ATGCAT 1 cut(s) 517
PaeR7I CTCGAG 1 cut(s) 441
PagI TCATGA 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 224
PctI GAATGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 2 cut(s) 64, 181
PleI GAGTC 2 cut(s) 21, 433
PpsI GAGTC 2 cut(s) 21, 433
PspFI CCCAGC 1 cut(s) 413
PspN4I GGNNCC 1 cut(s) 55
RsaI GTAC 2 cut(s) 329, 563
RsaNI GTAC 2 cut(s) 328, 562
RseI CAYNNNNRTG 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 2 cut(s) 63, 180
Sau3AI GATC 3 cut(s) 262, 421, 492
SchI GAGTC 2 cut(s) 22, 433
ScrFI CCNGG 1 cut(s) 299
SetI ASST 3 cut(s) 22, 59, 67
SfaNI GCATC 2 cut(s) 411, 502
Sfr274I CTCGAG 1 cut(s) 441
SlaI CTCGAG 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 3 cut(s) 160, 283, 406
SsiI CCGC 2 cut(s) 179, 198
SspMI CTAG 2 cut(s) 17, 486
StyD4I CCNGG 1 cut(s) 297
TaaI ACNGT 1 cut(s) 561
TaqI TCGA 5 cut(s) 83, 258, 293, 383, 442
TasI AATT 3 cut(s) 160, 283, 406
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 2 cut(s) 446, 469
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 1 cut(s) 355
XapI RAATTY 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 441
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 17, 486
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.